MD02G1137900.v1.1

TRAM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014389
Physical Location & Seq
Reverse (-)
11561047 .. 11564543
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1137900.v1.1.491

Sequence Viewer

Length: 1893 bp
ATGGCGACGTCGCTCTCGTCGTCCCTGTCCTCGATCCTGAACAACCCACACTGCGCGCTGCGCCTGAAGTTCCAGAGGCCGTCCTGCTTCACTCTCGGGTTCCTCAATTGGGGACCGGAGAAGTCCCGTTCCCGATGCTACCACCGCCGCCAGCAGCTGCAGCCTCGGCCACAAAGGAGGAGTCTTGGCCGCAGTAGCTCCAGAAGCTTCTCGAATTTTAGCCCCAACACTTCTTCTCTCTCCGGCCAGTCCGGTGGCCCCAGCCTCCGCCACTTTGTCGCTCAAGCTGCTCTCGCCGGTGCTGAAGCTCAGCCCCAGGATGTGAATTTGACTGCTACTGAAGTTGCTCCGATAGGACGTATCTATCACGAGACTTATGGGTGTCAAATGAATATTAATGACATGGAGATTGTTCTGTCTATTATGAAGAATGCTGGGTATAGTGAAGTCGTTGATGTCCCTGAGAATGCAGAGGTTATTTTTATTAACACCTGCGCTATCAGGGACAATGCGGAACAGAAGGTGTGGCAGCGGCTTAATTATTTTTGGTTTCTTAAGAGGGAATGGAAGGACAATGTTAAAATAGGGAGGTCGCAGTCCAAACGTCCTCCAAAAGTTGTAGTTTTGGGATGTATGGCGGAGAGGTTAAAGGACCAGATACTCGATTCAGACAAAATGGTTGATGTGGTTTGTGGGCCTGATGCGTACAGAGACTTGCCGAGATTATTAGAAGATGTGGACTATGGTCAGAAAGGAATCAATACTCTTCTTTCGCTGGAAGAGACTTATGCTGATATTAGCCCAGTTCGAATCTCCAAAAATTCAATTAGTGCTTTTGTTTCTGTTATGAGGGGATGTAACAATATGTGCTCATTTTGTATTGTTCCTTTCACTAGAGGCAGAGAGCGCTCGCGTTCTGTGGAATCTATTGTGCGGGAGGTGGGAGAGCTTTGGGAAGAAGGCGTGAAAGAGGTAACACTGCTAGGCCAGAATGTAAACAGCTACAATGATGCCTCTGAGAATGAAAAGGAAGTTGAAGCAGGAACTAATTGGAGATACAGCGAAGGCTTCTCCAGCATGTGCAAGGTGAAAAAAGTTGGTTCACGTTTTGCTGATCTCTTGGATCGACTGTCCACAGAATTTCCAGAGATGAGATTTCGATACACATCCCCACACCCTAAAGATTTCCCTGATGAGTTGCTGTATTTAATGCGAGACAGACCTAATATCTGCAGAAGTATCCATTTGCCTGCACAAAGTGGGAGTAGCACTGTGCTAGAAAGAATGCGTCGTGGCTATACCCGTGAGGCATACTTAGATCTTGTGCAAAAGATACGGAGAATTATTCCAGATGTGGGCATCAGCAGTGATTTCATATGCGGGTTTTGTGGGGAAACAGAGGAGGACCATGACGACACACTGAGCCTTGTAAAGGCTGTTGGCTACGATATGGCTTACATTTTTGCATATAGCATGAGGGATAAAACCCATGCAAATAGAAACTACGATGATGATGTTCCAGAGGATGTGAAGCAGAGGAGGCTGGCGGAACTTATCAAGGCTTTCCATAAGAGCACAGGTCAGTGCTACGACTCACAGGTGGGAACTACCCAACTTGTCTTAGTAGAAGGACCTAATAAGAAAGCTCCAGATACAGAACTCATGGGCAAAAGTGATAGAGGTCATAGAGTTTCTTTTGTTAACGTGCTGTTACCACATCGAGATGCTGGTTCAAATGAGGAAAGGAATCCCGTAGTGGGTGATTATGTTGAAGTTCGTATATTGAAATCAACAAGAACATCACTATTTGGAGAGGCTGTTGCGATAAGTAAATTGAGCTTGTTTTACAACAGTGTGGAGGAAGAAGCTGTTGCCTGTGGAAGTAGAAGTTGA

Protein Analysis

631

Amino Acids

71.14

Weight (kDa)

7.49

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0004 PF00919 120 - 232 4.8e-25 Uncharacterized protein family UPF0004
Radical_SAM PF04055 280 - 476 3.2e-27 Radical SAM superfamily
TRAM PF01938 532 - 607 2.6e-08 TRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36390
fragaria_vesca FvH4_1g12320
malus_domestica MD02G1137900.v1.1 MD15G1251600.v1.1
prunus_persica Prupe.7G162800_v2.0.a1
pyrus_communis pycom02g10930 pycom15g22170
rosa_chinensis RchiOBHm_Chr2g0100141
rosa_laevigata RLG00000016940
rosa_multiflora Rmu_sc0018554.1_g000003
rosa_roxburghii Rroxscaffold_2G00142530
rosa_rugosa Rorug02G0086000
rosa_samantha Rh2AG134700 Rh2BG138400 Rh2CG140200 Rh2DG140400
rosa_wichuraiana Rw2G010530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 500
AatII GACGTC 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 500
AccII CGCG 2 cut(s) 56, 913
AclWI GGATC 2 cut(s) 28, 1131
AcoI YGGCCR 3 cut(s) 167, 187, 244
AcsI RAATTY 4 cut(s) 214, 325, 820, 1139
AcuI CTGAAG 3 cut(s) 86, 324, 360
AcyI GRCGYC 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 1259
AfaI GTAC 1 cut(s) 707
AfeI AGCGCT 1 cut(s) 908
AfiI CCNNNNNNNGG 4 cut(s) 109, 1355, 1432, 1757
AflII CTTAAG 1 cut(s) 554
AgsI TTSAA 5 cut(s) 825, 1037, 1734, 1772, 1786
AjnI CCWGG 1 cut(s) 315
Alw21I GWGCWC 2 cut(s) 872, 1577
Alw26I GTCTC 4 cut(s) 365, 705, 776, 1209
AlwI GGATC 2 cut(s) 28, 1131
AlwNI CAGNNNCTG 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 95
Aor51HI AGCGCT 1 cut(s) 908
AoxI GGCC 7 cut(s) 77, 167, 187, 244, 256, 695, 985
ApeKI GCWGC 6 cut(s) 58, 154, 157, 160, 287, 529
ApoI RAATTY 4 cut(s) 214, 325, 820, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 5 cut(s) 56, 58, 63, 497, 909
AspS9I GGNCC 6 cut(s) 113, 257, 652, 695, 1405, 1631
AsuHPI GGTGA 2 cut(s) 1099, 1772
AsuII TTCGAA 1 cut(s) 808
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 4 cut(s) 113, 652, 1405, 1631
BauI CACGAG 1 cut(s) 368
Bbv12I GWGCWC 2 cut(s) 872, 1577
BbvI GCAGC 6 cut(s) 45, 144, 166, 172, 274, 541
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 1 cut(s) 317
BciVI GTATCC 1 cut(s) 1250
BcoDI GTCTC 4 cut(s) 365, 705, 776, 1209
BfaI CTAG 3 cut(s) 894, 983, 1277
BfmI CTRYAG 2 cut(s) 158, 1231
BfoI RGCGCY 1 cut(s) 910
BfrI CTTAAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 500
BfuI GTATCC 1 cut(s) 1250
BglII AGATCT 1 cut(s) 1318
BisI GCNGC 9 cut(s) 59, 148, 155, 158, 161, 190, 288, 530, 533
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 9 cut(s) 60, 149, 156, 159, 162, 191, 289, 531, 534
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 4 cut(s) 113, 652, 1405, 1631
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 6 cut(s) 113, 257, 652, 695, 1405, 1631
BmiI GGNNCC 3 cut(s) 101, 114, 259
BmrFI CCNGG 1 cut(s) 317
BmrI ACTGGG 1 cut(s) 797
BmsI GCATC 5 cut(s) 125, 691, 1000, 1368, 1714
BmuI ACTGGG 1 cut(s) 797
BoxI GACNNNNGTC 1 cut(s) 744
BpmI CTGGAG 3 cut(s) 184, 1057, 1632
Bpu1102I GCTNAGC 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 808
BpuEI CTTGAG 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 1165
BsaHI GRCGYC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 164, 315
BsaWI WCCGGW 2 cut(s) 115, 251
BsaXI ACNNNNNCTCC 2 cut(s) 1803, 1833
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 1355, 1432, 1757
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 2 cut(s) 247, 803
Bse8I GATNNNNATC 1 cut(s) 1165
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 2 cut(s) 164, 315
BseGI GGATG 5 cut(s) 325, 635, 860, 1166, 1531
BseJI GATNNNNATC 1 cut(s) 1165
BseLI CCNNNNNNNGG 4 cut(s) 109, 1355, 1432, 1757
BseMII CTCAG 4 cut(s) 323, 453, 1008, 1412
BseNI ACTGG 2 cut(s) 247, 803
BsePI GCGCGC 1 cut(s) 54
BseRI GAGGAG 3 cut(s) 193, 1415, 1552
BseXI GCAGC 6 cut(s) 45, 144, 166, 172, 274, 541
BseYI CCCAGC 2 cut(s) 260, 434
BsgI GTGCAG 1 cut(s) 1236
Bsh1236I CGCG 2 cut(s) 56, 913
BshFI GGCC 7 cut(s) 79, 169, 189, 246, 258, 697, 987
BsiHKAI GWGCWC 2 cut(s) 872, 1577
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 4 cut(s) 116, 243, 252, 297
BslFI GGGAC 5 cut(s) 7, 109, 126, 443, 518
BslI CCNNNNNNNGG 4 cut(s) 109, 1355, 1432, 1757
BsmAI GTCTC 4 cut(s) 365, 705, 776, 1209
BsmFI GGGAC 5 cut(s) 7, 109, 126, 443, 518
BsmI GAATGC 3 cut(s) 436, 472, 1290
BsnI GGCC 7 cut(s) 79, 169, 189, 246, 258, 697, 987
BsoBI CYCGRG 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 808
Bsp1286I GDGCHC 2 cut(s) 872, 1577
Bsp143I GATC 4 cut(s) 33, 1114, 1123, 1318
Bsp1720I GCTNAGC 1 cut(s) 309
BspANI GGCC 7 cut(s) 79, 169, 189, 246, 258, 697, 987
BspCNI CTCAG 4 cut(s) 322, 454, 1009, 1413
BspFNI CGCG 2 cut(s) 56, 913
BspLI GGNNCC 3 cut(s) 101, 114, 259
BspMAI CTGCAG 2 cut(s) 162, 1235
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 2 cut(s) 28, 1131
BspT104I TTCGAA 1 cut(s) 808
BspTI CTTAAG 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 2 cut(s) 247, 803
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 2 cut(s) 164, 315
BssHII GCGCGC 1 cut(s) 54
BssMI GATC 4 cut(s) 33, 1114, 1123, 1318
BssNI GRCGYC 1 cut(s) 8
BssSI CACGAG 1 cut(s) 368
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 3 cut(s) 1131, 1273, 1853
Bst6I CTCTTC 2 cut(s) 771, 774
BstACI GRCGYC 1 cut(s) 8
BstAFI CTTAAG 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 808
BstC8I GCNNGC 5 cut(s) 56, 152, 911, 1251, 1545
BstDEI CTNAG 6 cut(s) 309, 462, 1017, 1315, 1421, 1621
BstENI CCTNNNNNAGG 1 cut(s) 1430
BstF5I GGATG 5 cut(s) 325, 635, 860, 1166, 1531
BstFNI CGCG 2 cut(s) 56, 913
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 5 cut(s) 56, 58, 63, 497, 909
BstKTI GATC 4 cut(s) 36, 1117, 1126, 1321
BstMAI GTCTC 4 cut(s) 365, 705, 776, 1209
BstMBI GATC 4 cut(s) 33, 1114, 1123, 1318
BstNI CCWGG 1 cut(s) 317
BstNSI RCATGY 1 cut(s) 1081
BstPAI GACNNNNGTC 1 cut(s) 744
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 2 cut(s) 158, 1231
BstUI CGCG 2 cut(s) 56, 913
BstV1I GCAGC 6 cut(s) 45, 144, 166, 172, 274, 541
BstX2I RGATCY 1 cut(s) 1318
BstXI CCANNNNNNTGG 1 cut(s) 254
BstYI RGATCY 1 cut(s) 1318
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 7 cut(s) 79, 169, 189, 246, 258, 697, 987
BtsCI GGATG 5 cut(s) 325, 635, 860, 1166, 1531
BtsI GCAGTG 3 cut(s) 49, 977, 1372
BtsIMutI CAGTG 7 cut(s) 49, 977, 1269, 1372, 1418, 1589, 1858
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 5 cut(s) 56, 152, 911, 1251, 1545
CaiI CAGNNNCTG 1 cut(s) 157
CfoI GCGC 5 cut(s) 56, 58, 63, 497, 909
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 6 cut(s) 113, 257, 652, 695, 1405, 1631
CseI GACGC 1 cut(s) 1277
Csp6I GTAC 1 cut(s) 706
CviAII CATG 6 cut(s) 403, 1078, 1409, 1474, 1490, 1663
CviQI GTAC 1 cut(s) 706
DdeI CTNAG 6 cut(s) 309, 462, 1017, 1315, 1421, 1621
DpnI GATC 4 cut(s) 35, 1116, 1125, 1320
DpnII GATC 4 cut(s) 33, 1114, 1123, 1318
DraIII CACNNNGTG 1 cut(s) 1259
EaeI YGGCCR 3 cut(s) 167, 187, 244
Eam1104I CTCTTC 2 cut(s) 771, 774
EarI CTCTTC 2 cut(s) 771, 774
EciI GGCGGA 3 cut(s) 257, 653, 1562
Eco47I GGWCC 4 cut(s) 113, 652, 1405, 1631
Eco47III AGCGCT 1 cut(s) 908
Eco57I CTGAAG 3 cut(s) 86, 324, 360
Eco88I CYCGRG 1 cut(s) 95
EcoNI CCTNNNNNAGG 1 cut(s) 1430
EcoO109I RGGNCCY 1 cut(s) 1631
EcoRII CCWGG 1 cut(s) 315
FaeI CATG 6 cut(s) 406, 1081, 1412, 1477, 1493, 1666
FaqI GGGAC 5 cut(s) 7, 109, 126, 443, 518
FatI CATG 6 cut(s) 402, 1077, 1408, 1473, 1489, 1662
FauI CCCGC 2 cut(s) 927, 1373
FauNDI CATATG 1 cut(s) 1376
Fnu4HI GCNGC 9 cut(s) 59, 148, 155, 158, 161, 190, 288, 530, 533
FokI GGATG 5 cut(s) 332, 642, 867, 1153, 1538
Fsp4HI GCNGC 9 cut(s) 59, 148, 155, 158, 161, 190, 288, 530, 533
FspBI CTAG 3 cut(s) 894, 983, 1277
GlaI GCGC 5 cut(s) 55, 57, 62, 496, 908
GluI GCNGC 9 cut(s) 59, 148, 155, 158, 161, 190, 288, 530, 533
GsaI CCCAGC 2 cut(s) 264, 438
GsuI CTGGAG 3 cut(s) 184, 1057, 1632
HaeII RGCGCY 1 cut(s) 910
HaeIII GGCC 7 cut(s) 79, 169, 189, 246, 258, 697, 987
HapII CCGG 4 cut(s) 116, 243, 252, 297
HgaI GACGC 1 cut(s) 1277
HhaI GCGC 5 cut(s) 56, 58, 63, 497, 909
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 6 cut(s) 406, 1081, 1412, 1477, 1493, 1666
Hin6I GCGC 5 cut(s) 54, 56, 61, 495, 907
HinP1I GCGC 5 cut(s) 54, 56, 61, 495, 907
HincII GTYRAC 1 cut(s) 1702
HindII GTYRAC 1 cut(s) 1702
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 7 cut(s) 181, 665, 756, 810, 923, 1592, 1747
HpaI GTTAAC 1 cut(s) 1702
HpaII CCGG 4 cut(s) 116, 243, 252, 297
HphI GGTGA 2 cut(s) 1099, 1772
Hpy166II GTNNAC 5 cut(s) 739, 997, 1103, 1134, 1702
Hpy188I TCNGA 4 cut(s) 351, 670, 750, 1018
Hpy8I GTNNAC 5 cut(s) 739, 997, 1103, 1134, 1702
Hpy99I CGWCG 4 cut(s) 10, 13, 22, 1293
HpyAV CCTTC 5 cut(s) 514, 562, 953, 1058, 1622
HpyCH4III ACNGT 3 cut(s) 1131, 1273, 1853
HpyCH4IV ACGT 5 cut(s) 8, 358, 604, 1105, 1704
HpyCH4V TGCA 8 cut(s) 160, 470, 1083, 1233, 1253, 1327, 1466, 1493
HpyF3I CTNAG 6 cut(s) 309, 462, 1017, 1315, 1421, 1621
HpySE526I ACGT 5 cut(s) 8, 358, 604, 1105, 1704
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 6 cut(s) 406, 1081, 1412, 1477, 1493, 1666
HspAI GCGC 5 cut(s) 54, 56, 61, 495, 907
KspAI GTTAAC 1 cut(s) 1702
Kzo9I GATC 4 cut(s) 33, 1114, 1123, 1318
LmnI GCTCC 3 cut(s) 203, 352, 1651
Lsp1109I GCAGC 6 cut(s) 45, 144, 166, 172, 274, 541
LweI GCATC 5 cut(s) 125, 691, 1000, 1368, 1714
MaeI CTAG 3 cut(s) 894, 983, 1277
MaeII ACGT 5 cut(s) 8, 358, 604, 1105, 1704
MaeIII GTNAC 3 cut(s) 857, 973, 1710
MalI GATC 4 cut(s) 35, 1116, 1125, 1320
MboI GATC 4 cut(s) 33, 1114, 1123, 1318
MboII GAAGA 7 cut(s) 225, 439, 743, 758, 791, 968, 1874
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 1 cut(s) 1318
MhlI GDGCHC 2 cut(s) 872, 1577
MlyI GAGTC 2 cut(s) 190, 1586
MseI TTAA 8 cut(s) 396, 486, 537, 555, 579, 647, 1208, 1701
MslI CAYNNNNRTG 1 cut(s) 1722
MspA1I CMGCKG 2 cut(s) 157, 532
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 4 cut(s) 116, 243, 252, 297
MspR9I CCNGG 1 cut(s) 317
MteI GCGCNGCGC 1 cut(s) 59
MunI CAATTG 1 cut(s) 106
Mva1269I GAATGC 3 cut(s) 436, 472, 1290
MvaI CCWGG 1 cut(s) 317
MvnI CGCG 2 cut(s) 56, 913
NdeI CATATG 1 cut(s) 1376
NdeII GATC 4 cut(s) 33, 1114, 1123, 1318
NlaIII CATG 6 cut(s) 406, 1081, 1412, 1477, 1493, 1666
NlaIV GGNNCC 3 cut(s) 101, 114, 259
NmeAIII GCCGAG 2 cut(s) 145, 744
NspI RCATGY 1 cut(s) 1081
NspV TTCGAA 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 500
PauI GCGCGC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 17
PctI GAATGC 3 cut(s) 436, 472, 1290
PfeI GAWTC 5 cut(s) 665, 756, 810, 923, 1747
PkrI GCNGC 9 cut(s) 60, 149, 156, 159, 162, 191, 289, 531, 534
PleI GAGTC 2 cut(s) 189, 1586
PpsI GAGTC 2 cut(s) 189, 1586
PpuMI RGGWCCY 1 cut(s) 1631
PshAI GACNNNNGTC 1 cut(s) 744
PshBI ATTAAT 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 1631
Psp6I CCWGG 1 cut(s) 315
PspFI CCCAGC 2 cut(s) 260, 434
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 3 cut(s) 101, 114, 259
PspPI GGNCC 6 cut(s) 113, 257, 652, 695, 1405, 1631
PspPPI RGGWCCY 1 cut(s) 1631
PstI CTGCAG 2 cut(s) 162, 1235
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 1318
PteI GCGCGC 1 cut(s) 54
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 1 cut(s) 707
RsaNI GTAC 1 cut(s) 706
RseI CAYNNNNRTG 1 cut(s) 1722
SaqAI TTAA 8 cut(s) 396, 486, 537, 555, 579, 647, 1208, 1701
SatI GCNGC 9 cut(s) 59, 148, 155, 158, 161, 190, 288, 530, 533
Sau3AI GATC 4 cut(s) 33, 1114, 1123, 1318
Sau96I GGNCC 6 cut(s) 113, 257, 652, 695, 1405, 1631
SchI GAGTC 2 cut(s) 190, 1586
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 2 cut(s) 872, 1577
SfaNI GCATC 5 cut(s) 125, 691, 1000, 1368, 1714
SfcI CTRYAG 2 cut(s) 158, 1231
SfuI TTCGAA 1 cut(s) 808
SgrAI CRCCGGYG 1 cut(s) 296
SinI GGWCC 4 cut(s) 113, 652, 1405, 1631
SmiMI CAYNNNNRTG 1 cut(s) 1722
SmlI CTYRAG 2 cut(s) 282, 554
SmoI CTYRAG 2 cut(s) 282, 554
SspI AATATT 1 cut(s) 394
SspMI CTAG 3 cut(s) 894, 983, 1277
StyD4I CCNGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 1131, 1273, 1853
TaiI ACGT 5 cut(s) 11, 361, 607, 1108, 1707
TaqI TCGA 7 cut(s) 32, 212, 663, 808, 1126, 1159, 1720
TauI GCSGC 3 cut(s) 150, 192, 535
TfiI GAWTC 5 cut(s) 665, 756, 810, 923, 1747
Tru1I TTAA 8 cut(s) 396, 486, 537, 555, 579, 647, 1208, 1701
Tru9I TTAA 8 cut(s) 396, 486, 537, 555, 579, 647, 1208, 1701
TscAI CASTG 7 cut(s) 56, 984, 1276, 1372, 1425, 1589, 1858
TseI GCWGC 6 cut(s) 58, 154, 157, 160, 287, 529
TspDTI ATGAA 4 cut(s) 404, 440, 1038, 1363
TspGWI ACGGA 1 cut(s) 1351
TspRI CASTG 7 cut(s) 56, 984, 1276, 1372, 1425, 1589, 1858
Vha464I CTTAAG 1 cut(s) 554
VpaK11BI GGWCC 4 cut(s) 113, 652, 1405, 1631
VspI ATTAAT 1 cut(s) 396
XagI CCTNNNNNAGG 1 cut(s) 1430
XapI RAATTY 4 cut(s) 214, 325, 820, 1139
XceI RCATGY 1 cut(s) 1081
XspI CTAG 3 cut(s) 894, 983, 1277
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.