Prupe.7G162800_v2.0.a1

TRAM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0014389
Physical Location & Seq
Forward (+)
16799714 .. 16803208
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G162800.1

Sequence Viewer

Length: 1890 bp
ATGGCGTCGTCGCTCTCGTCCCTCTCCTCAATCCTGAACCAGCCTCACTGTGCTCTGCGCCTGAAGCTCCAGAGACCATGCTGCTTCACTCTCAGGTTTCTCAATTGGAGACCAGAAAAGTCCAGCTTCCGCTGCTACCACCACCACCAGCTGACGCCTCGGCCGCAAAGGAGGAGTCTTGGTCCCAATTTCTCCAGAAGCTTCTCTCAGTCTAACCCTAACCTTTCTTCTCTCCCGAACCAGTCTGAGAGCCCGAGCCTCCGCTACTTCGTCGCTCAGGCTGCTCTCACCGCTTCTGAAGCCCAGCCCCAGAATGTAACAGCTACGGAAGTTTCAGAAGTTCCTCCAATGGGCCGTATCTATCATGAGACTTATGGGTGTCAAATGAATATTAATGATATGGAGATCGTTCTGTCTATTATGAAGAATGCTGGGTATAGTGAAGTTGTTGATGTTCCTGAGAATGCAGAGGTTATTTTCATTAACACTTGTGCTATTAGGGACAATGCCGAACAGAAGGTGTGGCAGCGGCTTAATTACTTTTGGTTTCTCAAAAGGCACTGGAAGAGCAATGTTAAAATAGGGAGGTCGCAGTCCAAACGCCCTCCAAAAGTTGTAGTTTTGGGATGCATGGCCGAGAGGTTAAAGGACAAGATACTAGATGCAGACAAAATGGTTGATGTGGTTTGTGGGCCTGATGCATACAGAGACTTGCCAAGATTATTAGAAGATGTGGACTATGGGCAGAAAGGAATCAATACTCTTCTTTCACTGGAAGAGACTTACGCTGATATTAGTCCGGTTCGAATTGCTAAAAATTCAATTAGTGCTTTTGTTTCTGTTATGAGGGGTTGCAACAATATGTGCTCATTTTGCATTGTTCCTTTCACAAGAGGCAGAGAGCGCTCACGTCCTGTGGAATCAATTGTGAGGGAGGTGGCAGAGCTTTGGGAAGAAGGTGTGAAGGAGGTAACACTGCTAGGCCAGAATGTAAACAGCTATAATGATGCCTCTGAGTATGAAAAGGAAGTTGAAACAGGAACCAATTGGAGATATAGTGAAGGCTTTTCCAGCATGTGCAAGGTGAAAAAAGTAGGTTCTCGTTTTGCTGATCTCTTGGATCGACTTTCCACAGAATTTCCAGAGATGAGATTTAGATACACATCCCCGCACCCGAAAGATTTCCCTGATGAGCTACTGTATATAATGAGAGACAGACCCAATGTTTGCAAATCTATCCATTTGCCTGCTCAAACCGGAAGTAGCACAGTGCTAGAAAGAATGCGTCGGGGCTATAGCCGTGAGGCATACTTAGATCTTGTGCAAAAGATTCGGAGAATTATTCCAGATGTGGGCATCAGCAGTGATTTCATATGCGGGTTTTGTGGGGAGACAGAGGAGGAACATGCAGACACATTGAGCCTTGTAAAGGCTGTTGGTTATGATATGGCTTACATGTTTGCATATAGCATGAGGGAGAAAACTCATGCACATAGAAACTATGTGGATGATGTTCCAGAGAAAGTGAAGCAGAGGAGGCTGACCGAACTTATCAAGGCTTTCCGTGAGAGCACAGGTCAGTGTTATGACTCACAGGTGGGAACTACCCAACTCATCTTAGTAGAAGGACCCAATAAGAGAGCTCCGGATACAGAACTCATTGGCAAGAGTGATAGAGGCCATAGAGTTTCTTTTGCTAACGTGCTGTTACCACATCGAGATGCTGATTCAAATGAGGAAAGGAATCCCGTAGTTGGTGATTATGTTGAAGTGCGTATTTTGAAATCTACAAGAGCATCCCTATTTGGAGAGGCTGTTGCAATAAGTAAACTGAGCTTGTTTTACGACAATGTGGAGGAAGAAGCTGTTGCCTGTGGAAGTAGAAGTTGA

Protein Analysis

630

Amino Acids

71.57

Weight (kDa)

7.8

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36390
fragaria_vesca FvH4_1g12320
malus_domestica MD02G1137900.v1.1 MD15G1251600.v1.1
prunus_persica Prupe.7G162800_v2.0.a1
pyrus_communis pycom02g10930 pycom15g22170
rosa_chinensis RchiOBHm_Chr2g0100141
rosa_laevigata RLG00000016940
rosa_multiflora Rmu_sc0018554.1_g000003
rosa_roxburghii Rroxscaffold_2G00142530
rosa_rugosa Rorug02G0086000
rosa_samantha Rh2AG134700 Rh2BG138400 Rh2CG140200 Rh2DG140400
rosa_wichuraiana Rw2G010530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 1645
AciI CCGC 7 cut(s) 130, 164, 262, 291, 529, 1169, 1377
AclWI GGATC 1 cut(s) 1128
AcoI YGGCCR 2 cut(s) 161, 633
AcsI RAATTY 2 cut(s) 817, 1136
AcuI CTGAAG 2 cut(s) 83, 318
AcyI GRCGYC 2 cut(s) 5, 155
AfeI AGCGCT 1 cut(s) 905
AfiI CCNNNNNNNGG 4 cut(s) 350, 1352, 1429, 1754
AflIII ACRYGT 1 cut(s) 1455
AgsI TTSAA 5 cut(s) 822, 1034, 1731, 1769, 1783
AjiI CACGTC 1 cut(s) 911
Alw21I GWGCWC 4 cut(s) 55, 869, 1574, 1645
Alw26I GTCTC 7 cut(s) 67, 103, 362, 702, 773, 1206, 1385
AlwI GGATC 1 cut(s) 1128
Ama87I CYCGRG 1 cut(s) 253
Aor13HI TCCGGA 1 cut(s) 1645
Aor51HI AGCGCT 1 cut(s) 905
AoxI GGCC 6 cut(s) 161, 352, 633, 692, 982, 1678
ApeKI GCWGC 4 cut(s) 81, 132, 281, 526
ApoI RAATTY 2 cut(s) 817, 1136
ArsI GACNNNNNNTTYG 4 cut(s) 160, 192, 1209, 1241
AseI ATTAAT 1 cut(s) 393
Asp700I GAANNNNTTC 1 cut(s) 1181
AspLEI GCGC 2 cut(s) 60, 906
AspS9I GGNCC 4 cut(s) 182, 352, 692, 1628
AsuHPI GGTGA 3 cut(s) 280, 1096, 1769
AsuII TTCGAA 1 cut(s) 805
AvaI CYCGRG 1 cut(s) 253
AvaII GGWCC 2 cut(s) 182, 1628
BanII GRGCYC 2 cut(s) 254, 1645
Bbv12I GWGCWC 4 cut(s) 55, 869, 1574, 1645
BbvI GCAGC 4 cut(s) 68, 119, 268, 538
BceAI ACGGC 2 cut(s) 339, 1284
BcgI CGANNNNNNTGC 2 cut(s) 1313, 1347
BciVI GTATCC 1 cut(s) 1642
BcoDI GTCTC 7 cut(s) 67, 103, 362, 702, 773, 1206, 1385
BfaI CTAG 3 cut(s) 659, 980, 1274
BfmI CTRYAG 1 cut(s) 1294
BfoI RGCGCY 1 cut(s) 907
BfuI GTATCC 1 cut(s) 1642
BglII AGATCT 1 cut(s) 1315
BisI GCNGC 6 cut(s) 82, 133, 164, 282, 527, 530
BlsI GCNGC 6 cut(s) 83, 134, 165, 283, 528, 531
Bme18I GGWCC 2 cut(s) 182, 1628
BmeT110I CYCGRG 1 cut(s) 253
BmgBI CACGTC 1 cut(s) 911
BmgT120I GGNCC 4 cut(s) 182, 352, 692, 1628
BmiI GGNNCC 3 cut(s) 184, 1042, 1630
BmsI GCATC 7 cut(s) 617, 652, 688, 997, 1365, 1711, 1805
BpmI CTGGAG 2 cut(s) 53, 178
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 805
BsaBI GATNNNNATC 1 cut(s) 1162
BsaHI GRCGYC 2 cut(s) 5, 155
BsaI GGTCTC 2 cut(s) 67, 103
BsaJI CCNNGG 1 cut(s) 158
BsaWI WCCGGW 3 cut(s) 799, 1256, 1645
BsaXI ACNNNNNCTCC 6 cut(s) 166, 196, 1042, 1072, 1800, 1830
Bsc4I CCNNNNNNNGG 4 cut(s) 350, 1352, 1429, 1754
Bse1I ACTGG 3 cut(s) 241, 566, 777
Bse3DI GCAATG 1 cut(s) 577
Bse8I GATNNNNATC 1 cut(s) 1162
BseAI TCCGGA 1 cut(s) 1645
BseDI CCNNGG 1 cut(s) 158
BseGI GGATG 4 cut(s) 632, 1163, 1513, 1796
BseJI GATNNNNATC 1 cut(s) 1162
BseLI CCNNNNNNNGG 4 cut(s) 350, 1352, 1429, 1754
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 7 cut(s) 106, 221, 237, 290, 450, 1005, 1823
BseNI ACTGG 3 cut(s) 241, 566, 777
BseRI GAGGAG 4 cut(s) 16, 187, 1412, 1549
BseX3I CGGCCG 1 cut(s) 161
BseXI GCAGC 4 cut(s) 68, 119, 268, 538
BseYI CCCAGC 2 cut(s) 303, 431
Bsh1285I CGRYCG 1 cut(s) 164
BshFI GGCC 6 cut(s) 163, 354, 635, 694, 984, 1680
BsiEI CGRYCG 1 cut(s) 164
BsiHKAI GWGCWC 4 cut(s) 55, 869, 1574, 1645
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 3 cut(s) 800, 1257, 1646
BslFI GGGAC 3 cut(s) 4, 168, 515
BslI CCNNNNNNNGG 4 cut(s) 350, 1352, 1429, 1754
BsmAI GTCTC 7 cut(s) 67, 103, 362, 702, 773, 1206, 1385
BsmFI GGGAC 3 cut(s) 4, 168, 515
BsmI GAATGC 3 cut(s) 433, 469, 1287
BsnI GGCC 6 cut(s) 163, 354, 635, 694, 984, 1680
Bso31I GGTCTC 2 cut(s) 67, 103
BsoBI CYCGRG 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 805
Bsp1286I GDGCHC 5 cut(s) 55, 254, 869, 1574, 1645
Bsp13I TCCGGA 1 cut(s) 1645
Bsp143I GATC 4 cut(s) 405, 1111, 1120, 1315
BspACI CCGC 7 cut(s) 130, 164, 262, 291, 529, 1169, 1377
BspANI GGCC 6 cut(s) 163, 354, 635, 694, 984, 1680
BspCNI CTCAG 7 cut(s) 105, 220, 238, 289, 451, 1006, 1824
BspEI TCCGGA 1 cut(s) 1645
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 3 cut(s) 184, 1042, 1630
BspPI GGATC 1 cut(s) 1128
BspQI GCTCTTC 1 cut(s) 560
BspT104I TTCGAA 1 cut(s) 805
BspTNI GGTCTC 2 cut(s) 67, 103
BsrDI GCAATG 1 cut(s) 577
BsrI ACTGG 3 cut(s) 241, 566, 777
BssECI CCNNGG 1 cut(s) 158
BssMI GATC 4 cut(s) 405, 1111, 1120, 1315
BssNI GRCGYC 2 cut(s) 5, 155
Bst4CI ACNGT 3 cut(s) 50, 1200, 1270
Bst6I CTCTTC 3 cut(s) 560, 768, 771
BstACI GRCGYC 2 cut(s) 5, 155
BstBI TTCGAA 1 cut(s) 805
BstC8I GCNNGC 1 cut(s) 1248
BstDEI CTNAG 9 cut(s) 92, 207, 246, 276, 459, 1014, 1312, 1618, 1832
BstENI CCTNNNNNAGG 1 cut(s) 1427
BstF5I GGATG 4 cut(s) 632, 1163, 1513, 1796
BstH2I RGCGCY 1 cut(s) 907
BstHHI GCGC 2 cut(s) 60, 906
BstKTI GATC 4 cut(s) 408, 1114, 1123, 1318
BstMAI GTCTC 7 cut(s) 67, 103, 362, 702, 773, 1206, 1385
BstMBI GATC 4 cut(s) 405, 1111, 1120, 1315
BstMCI CGRYCG 1 cut(s) 164
BstNSI RCATGY 3 cut(s) 1078, 1409, 1459
BstSFI CTRYAG 1 cut(s) 1294
BstV1I GCAGC 4 cut(s) 68, 119, 268, 538
BstX2I RGATCY 1 cut(s) 1315
BstYI RGATCY 1 cut(s) 1315
BstZI CGGCCG 1 cut(s) 161
BsuI GTATCC 1 cut(s) 1642
BsuRI GGCC 6 cut(s) 163, 354, 635, 694, 984, 1680
BtrI CACGTC 1 cut(s) 911
BtsCI GGATG 4 cut(s) 632, 1163, 1513, 1796
BtsI GCAGTG 2 cut(s) 974, 1369
BtsIMutI CAGTG 7 cut(s) 46, 559, 770, 974, 1275, 1369, 1586
Cac8I GCNNGC 1 cut(s) 1248
CciI TCATGA 1 cut(s) 364
CfoI GCGC 2 cut(s) 60, 906
Cfr13I GGNCC 4 cut(s) 182, 352, 692, 1628
CseI GACGC 2 cut(s) 163, 1274
CviAII CATG 8 cut(s) 78, 365, 631, 1075, 1406, 1456, 1471, 1487
DdeI CTNAG 9 cut(s) 92, 207, 246, 276, 459, 1014, 1312, 1618, 1832
DpnI GATC 4 cut(s) 407, 1113, 1122, 1317
DpnII GATC 4 cut(s) 405, 1111, 1120, 1315
EaeI YGGCCR 2 cut(s) 161, 633
EagI CGGCCG 1 cut(s) 161
Eam1104I CTCTTC 3 cut(s) 560, 768, 771
EarI CTCTTC 3 cut(s) 560, 768, 771
Ecl136II GAGCTC 1 cut(s) 1643
EclXI CGGCCG 1 cut(s) 161
Eco24I GRGCYC 2 cut(s) 254, 1645
Eco31I GGTCTC 2 cut(s) 67, 103
Eco47I GGWCC 2 cut(s) 182, 1628
Eco47III AGCGCT 1 cut(s) 905
Eco52I CGGCCG 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 1643
Eco57I CTGAAG 2 cut(s) 83, 318
Eco88I CYCGRG 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 1643
EcoNI CCTNNNNNAGG 1 cut(s) 1427
EcoO109I RGGNCCY 1 cut(s) 1628
EcoT22I ATGCAT 2 cut(s) 632, 703
EcoT38I GRGCYC 2 cut(s) 254, 1645
FaeI CATG 8 cut(s) 81, 368, 634, 1078, 1409, 1459, 1474, 1490
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 3 cut(s) 4, 168, 515
FatI CATG 8 cut(s) 77, 364, 630, 1074, 1405, 1455, 1470, 1486
FauI CCCGC 2 cut(s) 1176, 1370
FauNDI CATATG 1 cut(s) 1373
Fnu4HI GCNGC 6 cut(s) 82, 133, 164, 282, 527, 530
FokI GGATG 4 cut(s) 639, 1150, 1520, 1783
FriOI GRGCYC 2 cut(s) 254, 1645
Fsp4HI GCNGC 6 cut(s) 82, 133, 164, 282, 527, 530
FspBI CTAG 3 cut(s) 659, 980, 1274
GlaI GCGC 2 cut(s) 59, 905
GluI GCNGC 6 cut(s) 82, 133, 164, 282, 527, 530
GsaI CCCAGC 2 cut(s) 307, 435
GsuI CTGGAG 2 cut(s) 53, 178
HaeII RGCGCY 1 cut(s) 907
HaeIII GGCC 6 cut(s) 163, 354, 635, 694, 984, 1680
HapII CCGG 3 cut(s) 800, 1257, 1646
HgaI GACGC 2 cut(s) 163, 1274
HhaI GCGC 2 cut(s) 60, 906
Hin1I GRCGYC 2 cut(s) 5, 155
Hin1II CATG 8 cut(s) 81, 368, 634, 1078, 1409, 1459, 1474, 1490
Hin6I GCGC 2 cut(s) 58, 904
HinP1I GCGC 2 cut(s) 58, 904
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 7 cut(s) 175, 753, 920, 1330, 1589, 1727, 1744
HpaII CCGG 3 cut(s) 800, 1257, 1646
HphI GGTGA 3 cut(s) 280, 1096, 1769
Hpy166II GTNNAC 3 cut(s) 736, 994, 1829
Hpy188I TCNGA 5 cut(s) 247, 298, 337, 1015, 1335
Hpy8I GTNNAC 3 cut(s) 736, 994, 1829
Hpy99I CGWCG 4 cut(s) 10, 13, 275, 1290
HpyAV CCTTC 5 cut(s) 511, 950, 958, 1055, 1619
HpyCH4III ACNGT 3 cut(s) 50, 1200, 1270
HpyCH4IV ACGT 2 cut(s) 910, 1701
HpyF3I CTNAG 9 cut(s) 92, 207, 246, 276, 459, 1014, 1312, 1618, 1832
HpySE526I ACGT 2 cut(s) 910, 1701
Hsp92I GRCGYC 2 cut(s) 5, 155
Hsp92II CATG 8 cut(s) 81, 368, 634, 1078, 1409, 1459, 1474, 1490
HspAI GCGC 2 cut(s) 58, 904
Kpn2I TCCGGA 1 cut(s) 1645
Kzo9I GATC 4 cut(s) 405, 1111, 1120, 1315
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 2 cut(s) 72, 1648
Lsp1109I GCAGC 4 cut(s) 68, 119, 268, 538
LweI GCATC 7 cut(s) 617, 652, 688, 997, 1365, 1711, 1805
MaeI CTAG 3 cut(s) 659, 980, 1274
MaeII ACGT 2 cut(s) 910, 1701
MaeIII GTNAC 3 cut(s) 316, 970, 1707
MalI GATC 4 cut(s) 407, 1113, 1122, 1317
MboI GATC 4 cut(s) 405, 1111, 1120, 1315
MboII GAAGA 8 cut(s) 219, 436, 577, 740, 755, 788, 965, 1871
MfeI CAATTG 3 cut(s) 103, 924, 1045
MflI RGATCY 1 cut(s) 1315
MhlI GDGCHC 5 cut(s) 55, 254, 869, 1574, 1645
MlyI GAGTC 2 cut(s) 184, 1583
Mph1103I ATGCAT 2 cut(s) 632, 703
MroI TCCGGA 1 cut(s) 1645
MroXI GAANNNNTTC 1 cut(s) 1181
MseI TTAA 5 cut(s) 393, 483, 534, 576, 644
MslI CAYNNNNRTG 1 cut(s) 1719
MspA1I CMGCKG 3 cut(s) 132, 151, 529
MspI CCGG 3 cut(s) 800, 1257, 1646
MunI CAATTG 3 cut(s) 103, 924, 1045
Mva1269I GAATGC 3 cut(s) 433, 469, 1287
NdeI CATATG 1 cut(s) 1373
NdeII GATC 4 cut(s) 405, 1111, 1120, 1315
NlaIII CATG 8 cut(s) 81, 368, 634, 1078, 1409, 1459, 1474, 1490
NlaIV GGNNCC 3 cut(s) 184, 1042, 1630
NmeAIII GCCGAG 2 cut(s) 139, 661
NsiI ATGCAT 2 cut(s) 632, 703
NspI RCATGY 3 cut(s) 1078, 1409, 1459
NspV TTCGAA 1 cut(s) 805
PagI TCATGA 1 cut(s) 364
PciI ACATGT 1 cut(s) 1455
PciSI GCTCTTC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 3 cut(s) 433, 469, 1287
PdmI GAANNNNTTC 1 cut(s) 1181
PfeI GAWTC 5 cut(s) 753, 920, 1330, 1727, 1744
PkrI GCNGC 6 cut(s) 83, 134, 165, 283, 528, 531
PleI GAGTC 2 cut(s) 183, 1583
PpsI GAGTC 2 cut(s) 183, 1583
PpuMI RGGWCCY 1 cut(s) 1628
PscI ACATGT 1 cut(s) 1455
PshBI ATTAAT 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 1645
Psp5II RGGWCCY 1 cut(s) 1628
PspFI CCCAGC 2 cut(s) 303, 431
PspN4I GGNNCC 3 cut(s) 184, 1042, 1630
PspPI GGNCC 4 cut(s) 182, 352, 692, 1628
PspPPI RGGWCCY 1 cut(s) 1628
PsuI RGATCY 1 cut(s) 1315
PvuII CAGCTG 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 1719
SacI GAGCTC 1 cut(s) 1645
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 5 cut(s) 393, 483, 534, 576, 644
SatI GCNGC 6 cut(s) 82, 133, 164, 282, 527, 530
Sau3AI GATC 4 cut(s) 405, 1111, 1120, 1315
Sau96I GGNCC 4 cut(s) 182, 352, 692, 1628
SchI GAGTC 2 cut(s) 184, 1583
SduI GDGCHC 5 cut(s) 55, 254, 869, 1574, 1645
SfaNI GCATC 7 cut(s) 617, 652, 688, 997, 1365, 1711, 1805
SfcI CTRYAG 1 cut(s) 1294
SfuI TTCGAA 1 cut(s) 805
SinI GGWCC 2 cut(s) 182, 1628
SmiMI CAYNNNNRTG 1 cut(s) 1719
SsiI CCGC 7 cut(s) 130, 164, 262, 291, 529, 1169, 1377
SspI AATATT 1 cut(s) 391
SspMI CTAG 3 cut(s) 659, 980, 1274
SstI GAGCTC 1 cut(s) 1645
TaaI ACNGT 3 cut(s) 50, 1200, 1270
TaiI ACGT 2 cut(s) 913, 1704
TaqI TCGA 3 cut(s) 805, 1123, 1717
TaqII GACCGA 1 cut(s) 1559
TauI GCSGC 2 cut(s) 166, 532
TfiI GAWTC 5 cut(s) 753, 920, 1330, 1727, 1744
Tru1I TTAA 5 cut(s) 393, 483, 534, 576, 644
Tru9I TTAA 5 cut(s) 393, 483, 534, 576, 644
TscAI CASTG 7 cut(s) 53, 566, 777, 981, 1275, 1369, 1586
TseI GCWGC 4 cut(s) 81, 132, 281, 526
TspDTI ATGAA 5 cut(s) 401, 437, 469, 1035, 1360
TspGWI ACGGA 2 cut(s) 341, 1553
TspRI CASTG 7 cut(s) 53, 566, 777, 981, 1275, 1369, 1586
VpaK11BI GGWCC 2 cut(s) 182, 1628
VspI ATTAAT 1 cut(s) 393
XagI CCTNNNNNAGG 1 cut(s) 1427
XapI RAATTY 2 cut(s) 817, 1136
XceI RCATGY 3 cut(s) 1078, 1409, 1459
XmnI GAANNNNTTC 1 cut(s) 1181
XspI CTAG 3 cut(s) 659, 980, 1274
Zsp2I ATGCAT 2 cut(s) 632, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.