pycom02g10930

TRAM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014389
Physical Location & Seq
Reverse (-)
7730807 .. 7734069
3263 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g10930.2

Sequence Viewer

Length: 2004 bp
ATGTCGTCTCTCACACTATTACGGGTTTCTTTCCTGACAGTCGTAAATCGTTACTCCTATCCAACCCTCTCTGACGCAGAGAAGCTGAGAAGCGCGAACGCAATCTCGCCAATGGCGTCGTCGCTCTCGTCGTCCCTGTCCTCGATCCTGAACAACCCACACTGCGCGCTGCGCCTGAAGTTCCAGAGGCCGTCCTGCTTCACTCTCGGGTTCCTCAATTGGGGACCGGAGAAGTCCGGTTCCCGATGCTACCACCGCGGCCAGCAGCTGCAGCCTCGGCCACAAAGGAGGAGTCTTGGCCGCAGTAGCTCCAGAAGCTTCTCGAATTCTAGTCCCAACGCTTCTTCTCTCTCCGGCCAGTCCGGTGGCCCCAGCCTCCGCCACTTCGTCGCTCAAGCTGCTCTCACCGGTGCTGAAGCCCAGCCCCAGAATGTGAATGTGACTGCTACAGAAGTTGCTCCAATAGGTCGTATCTATCACGAGACTTATGGGTGTCAAATGAATATTAATGACATGGAGATTGTTCTGTCTATTATGAAGAATGCTGGGTATAGTGAAGTCGTTGATGTCCCTGAGAATGCAGAGGTTATTTTTATTAACACCTGCGCTATCAGGGACAATGCAGAACAGAAGGTTTGGCAGCGGCTTAATTATTTTTGGTTTCTTAAGAGGGAATGGAAGGACAATGTTAAAATAGGGAGGTCGCAGTCCAAACGTCCTCCAAAAGTTGTAGTTTTGGGATGTATGGCGGAGAGGTTAAAGGACCAGATACTCGATTCAGACAAAATGGTTGATGTGGTTTGTGGGCCTGATGCATACAGAGACTTGCCGAGATTATTAGAAGATGTGGACTATGGTCAGAAAGGAATCAATACTCTTCTTTCGCTGGAAGAGACTTATGCTGATATTAGTCCTGTTCGAATCTCCAAAAATTCAATTAGTGCTTTTGTTTCTGTTATGAGGGGATGTAACAATATGTGCTCATTTTGCATTGTTCCTTTCACTAGAGGCAGAGAGCGCTCGCGTCCTGTGGAATCTATTGTGAGGGAGGTGGGAGAGCTTTGGGAAGAAGGCGTGAAAGAGGTAACACTGCTAGGCCAGAATGTAAACAGCTACAATGATGCCTCTGAGAATGAAAAGGAAGTTGAAGCAGGAACTAATTGGCGATACAGCGAAGGCTTCTCCAGCACGTGCAAGGTGAAAAAAGTTGGTTTGCGTTTTGCTGATCTCTTGGATCGACTGTCCACAGAATATCCAGAGATGAGATTCCGATACACATCCCCACACCCTAAAGATTTCCCTGATGAGTTGCTGTATTTAATGCGAGACAGACCTAATATCTGCAGAAGTATCCATTTGCCTGCACAGACTGGGAGTACCGCAGTGCTAGAAAGAATGCGTCGTGGCTATACCCGTGAGGCATACTTGGATCTTGTGCAAAAGATACGGAGAATTATTCCAGATGTGGGCATCAGCAGTGATTTCATATGTGGGTTTTGTGGGGAAACAGAGGAGGACCATGACGACACACTGAGCCTTGTAAAGGCTGTTGGCTACGATATGGCTTACATTTTTGCATATAGCATGAGGGATAAAACCCATGCAAATAGAAACTACGATGATGATGTTCCAGAGGATGTGAAGCAGAGGAGGCTGGCGGAACTTATCAAGGCTTTCCATAAGAGCACAGGTCAGTGTTACGACTCACAGGTGGGAACTACCCAACTTGTCTTAGTAGAAGGACCTAATAAGAAAGCTCCAGATACAGAACTCATGGGCAAGAGTGATAGAGGTCATAGAGTTTCTTTTGTTAACGTGCTGTTAACACATCGAGATGCTGATTCAAATGAGGAAAGGAATCCCGTGGTGGGTGATTATGTTGAAGTTCGTATATTGAAATCAACAAGAACATCACTATTTGGAGAGGCTGTTGCGATAAGTAAATTGAGCTTGTTTTACAACAATGTGGAGGAAGAAGCTGTTGTCTGTGGAAGCAGAAGTTGA

Protein Analysis

668

Amino Acids

75.07

Weight (kDa)

7.48

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014389)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36390
fragaria_vesca FvH4_1g12320
malus_domestica MD02G1137900.v1.1 MD15G1251600.v1.1
prunus_persica Prupe.7G162800_v2.0.a1
pyrus_communis pycom02g10930 pycom15g22170
rosa_chinensis RchiOBHm_Chr2g0100141
rosa_laevigata RLG00000016940
rosa_multiflora Rmu_sc0018554.1_g000003
rosa_roxburghii Rroxscaffold_2G00142530
rosa_rugosa Rorug02G0086000
rosa_samantha Rh2AG134700 Rh2BG138400 Rh2CG140200 Rh2DG140400
rosa_wichuraiana Rw2G010530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 611
Acc36I ACCTGC 1 cut(s) 611
AccII CGCG 4 cut(s) 95, 167, 258, 1024
AciI CCGC 8 cut(s) 256, 258, 301, 379, 643, 749, 1380, 1658
AclWI GGATC 3 cut(s) 139, 1242, 1437
AcoI YGGCCR 4 cut(s) 259, 278, 298, 355
AcsI RAATTY 2 cut(s) 325, 931
AcuI CTGAAG 2 cut(s) 197, 435
AcvI CACGTG 1 cut(s) 1191
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 1 cut(s) 1378
AfeI AGCGCT 1 cut(s) 1019
AfiI CCNNNNNNNGG 4 cut(s) 220, 1466, 1543, 1868
AflII CTTAAG 1 cut(s) 665
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 5 cut(s) 936, 1148, 1845, 1883, 1897
Alw21I GWGCWC 2 cut(s) 983, 1688
Alw26I GTCTC 5 cut(s) 12, 476, 816, 887, 1320
AlwI GGATC 3 cut(s) 139, 1242, 1437
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 206
Aor51HI AGCGCT 1 cut(s) 1019
AoxI GGCC 8 cut(s) 188, 259, 278, 298, 355, 367, 806, 1096
ApeKI GCWGC 6 cut(s) 169, 265, 268, 271, 398, 640
ApoI RAATTY 2 cut(s) 325, 931
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AseI ATTAAT 1 cut(s) 507
AsiGI ACCGGT 1 cut(s) 407
AspLEI GCGC 6 cut(s) 95, 167, 169, 174, 608, 1020
AspS9I GGNCC 6 cut(s) 224, 368, 763, 806, 1516, 1742
AsuHPI GGTGA 3 cut(s) 397, 1210, 1883
AsuII TTCGAA 1 cut(s) 919
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 4 cut(s) 224, 763, 1516, 1742
BauI CACGAG 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 1191
Bbv12I GWGCWC 2 cut(s) 983, 1688
BbvI GCAGC 6 cut(s) 156, 255, 277, 283, 385, 652
BceAI ACGGC 1 cut(s) 175
BciVI GTATCC 1 cut(s) 1361
BcoDI GTCTC 5 cut(s) 12, 476, 816, 887, 1320
BfaI CTAG 4 cut(s) 330, 1005, 1094, 1388
BfmI CTRYAG 3 cut(s) 269, 447, 1342
BfoI RGCGCY 1 cut(s) 1021
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 611
BfuI GTATCC 1 cut(s) 1361
BisI GCNGC 9 cut(s) 170, 259, 266, 269, 272, 301, 399, 641, 644
BlsI GCNGC 9 cut(s) 171, 260, 267, 270, 273, 302, 400, 642, 645
Bme18I GGWCC 4 cut(s) 224, 763, 1516, 1742
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 6 cut(s) 224, 368, 763, 806, 1516, 1742
BmiI GGNNCC 4 cut(s) 212, 225, 241, 370
BmrI ACTGGG 1 cut(s) 1380
BmsI GCATC 5 cut(s) 236, 802, 1111, 1479, 1825
BmuI ACTGGG 1 cut(s) 1380
BoxI GACNNNNGTC 1 cut(s) 855
BpmI CTGGAG 3 cut(s) 295, 1168, 1743
Bpu14I TTCGAA 1 cut(s) 919
BpuEI CTTGAG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 1191
BsaBI GATNNNNATC 1 cut(s) 1276
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 256, 275, 1863
BsaWI WCCGGW 4 cut(s) 226, 236, 362, 407
BsaXI ACNNNNNCTCC 2 cut(s) 1914, 1944
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 1466, 1543, 1868
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 2 cut(s) 358, 1375
Bse8I GATNNNNATC 1 cut(s) 1276
BseDI CCNNGG 3 cut(s) 256, 275, 1863
BseGI GGATG 4 cut(s) 746, 971, 1277, 1642
BseJI GATNNNNATC 1 cut(s) 1276
BseLI CCNNNNNNNGG 4 cut(s) 220, 1466, 1543, 1868
BseMII CTCAG 4 cut(s) 77, 564, 1119, 1523
BseNI ACTGG 2 cut(s) 358, 1375
BsePI GCGCGC 1 cut(s) 165
BseRI GAGGAG 3 cut(s) 304, 1526, 1663
BseXI GCAGC 6 cut(s) 156, 255, 277, 283, 385, 652
BseYI CCCAGC 3 cut(s) 371, 420, 545
BsgI GTGCAG 1 cut(s) 1347
Bsh1236I CGCG 4 cut(s) 95, 167, 258, 1024
BshFI GGCC 8 cut(s) 190, 261, 280, 300, 357, 369, 808, 1098
BshTI ACCGGT 1 cut(s) 407
BsiHKAI GWGCWC 2 cut(s) 983, 1688
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 5 cut(s) 227, 237, 354, 363, 408
BslFI GGGAC 5 cut(s) 118, 237, 318, 554, 629
BslI CCNNNNNNNGG 4 cut(s) 220, 1466, 1543, 1868
BsmAI GTCTC 5 cut(s) 12, 476, 816, 887, 1320
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 5 cut(s) 118, 237, 318, 554, 629
BsmI GAATGC 3 cut(s) 547, 583, 1401
BsnI GGCC 8 cut(s) 190, 261, 280, 300, 357, 369, 808, 1098
BsoBI CYCGRG 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 919
Bsp1286I GDGCHC 2 cut(s) 983, 1688
Bsp143I GATC 4 cut(s) 144, 1225, 1234, 1429
BspACI CCGC 8 cut(s) 256, 258, 301, 379, 643, 749, 1380, 1658
BspANI GGCC 8 cut(s) 190, 261, 280, 300, 357, 369, 808, 1098
BspCNI CTCAG 4 cut(s) 78, 565, 1120, 1524
BspFNI CGCG 4 cut(s) 95, 167, 258, 1024
BspLI GGNNCC 4 cut(s) 212, 225, 241, 370
BspMAI CTGCAG 2 cut(s) 273, 1346
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 3 cut(s) 139, 1242, 1437
BspT104I TTCGAA 1 cut(s) 919
BspTI CTTAAG 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 2 cut(s) 358, 1375
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 256, 275, 1863
BssHII GCGCGC 1 cut(s) 165
BssMI GATC 4 cut(s) 144, 1225, 1234, 1429
BssNI GRCGYC 1 cut(s) 116
BssSI CACGAG 1 cut(s) 479
Bst2BI CACGAG 1 cut(s) 479
Bst4CI ACNGT 2 cut(s) 40, 1242
Bst6I CTCTTC 2 cut(s) 882, 885
BstACI GRCGYC 1 cut(s) 116
BstAFI CTTAAG 1 cut(s) 665
BstBAI YACGTR 1 cut(s) 1191
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 5 cut(s) 167, 263, 1022, 1362, 1656
BstDEI CTNAG 5 cut(s) 86, 573, 1128, 1532, 1732
BstDSI CCRYGG 2 cut(s) 256, 1863
BstENI CCTNNNNNAGG 1 cut(s) 1541
BstF5I GGATG 4 cut(s) 746, 971, 1277, 1642
BstFNI CGCG 4 cut(s) 95, 167, 258, 1024
BstH2I RGCGCY 1 cut(s) 1021
BstHHI GCGC 6 cut(s) 95, 167, 169, 174, 608, 1020
BstKTI GATC 4 cut(s) 147, 1228, 1237, 1432
BstMAI GTCTC 5 cut(s) 12, 476, 816, 887, 1320
BstMBI GATC 4 cut(s) 144, 1225, 1234, 1429
BstPAI GACNNNNGTC 1 cut(s) 855
BstSFI CTRYAG 3 cut(s) 269, 447, 1342
BstUI CGCG 4 cut(s) 95, 167, 258, 1024
BstV1I GCAGC 6 cut(s) 156, 255, 277, 283, 385, 652
BstX2I RGATCY 1 cut(s) 1429
BstXI CCANNNNNNTGG 1 cut(s) 365
BstYI RGATCY 1 cut(s) 1429
BsuI GTATCC 1 cut(s) 1361
BsuRI GGCC 8 cut(s) 190, 261, 280, 300, 357, 369, 808, 1098
BtgI CCRYGG 2 cut(s) 256, 1863
BtsCI GGATG 4 cut(s) 746, 971, 1277, 1642
BtsI GCAGTG 4 cut(s) 160, 1088, 1389, 1483
BtsIMutI CAGTG 6 cut(s) 160, 1088, 1389, 1483, 1529, 1700
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 5 cut(s) 167, 263, 1022, 1362, 1656
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 6 cut(s) 95, 167, 169, 174, 608, 1020
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 6 cut(s) 224, 368, 763, 806, 1516, 1742
Cfr42I CCGCGG 1 cut(s) 259
CseI GACGC 4 cut(s) 83, 105, 1013, 1388
Csp6I GTAC 1 cut(s) 1377
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 5 cut(s) 514, 1520, 1585, 1601, 1774
CviQI GTAC 1 cut(s) 1377
DdeI CTNAG 5 cut(s) 86, 573, 1128, 1532, 1732
DpnI GATC 4 cut(s) 146, 1227, 1236, 1431
DpnII GATC 4 cut(s) 144, 1225, 1234, 1429
EaeI YGGCCR 4 cut(s) 259, 278, 298, 355
Eam1104I CTCTTC 2 cut(s) 882, 885
EarI CTCTTC 2 cut(s) 882, 885
EciI GGCGGA 3 cut(s) 368, 764, 1673
Eco47I GGWCC 4 cut(s) 224, 763, 1516, 1742
Eco47III AGCGCT 1 cut(s) 1019
Eco57I CTGAAG 2 cut(s) 197, 435
Eco72I CACGTG 1 cut(s) 1191
Eco88I CYCGRG 1 cut(s) 206
EcoNI CCTNNNNNAGG 1 cut(s) 1541
EcoO109I RGGNCCY 1 cut(s) 1742
EcoRI GAATTC 1 cut(s) 325
EcoT22I ATGCAT 1 cut(s) 817
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 5 cut(s) 517, 1523, 1588, 1604, 1777
FaqI GGGAC 5 cut(s) 118, 237, 318, 554, 629
FatI CATG 5 cut(s) 513, 1519, 1584, 1600, 1773
FauNDI CATATG 1 cut(s) 1487
Fnu4HI GCNGC 9 cut(s) 170, 259, 266, 269, 272, 301, 399, 641, 644
FokI GGATG 4 cut(s) 753, 978, 1264, 1649
Fsp4HI GCNGC 9 cut(s) 170, 259, 266, 269, 272, 301, 399, 641, 644
FspBI CTAG 4 cut(s) 330, 1005, 1094, 1388
GlaI GCGC 6 cut(s) 94, 166, 168, 173, 607, 1019
GluI GCNGC 9 cut(s) 170, 259, 266, 269, 272, 301, 399, 641, 644
GsaI CCCAGC 3 cut(s) 375, 424, 549
GsuI CTGGAG 3 cut(s) 295, 1168, 1743
HaeII RGCGCY 1 cut(s) 1021
HaeIII GGCC 8 cut(s) 190, 261, 280, 300, 357, 369, 808, 1098
HapII CCGG 5 cut(s) 227, 237, 354, 363, 408
HgaI GACGC 4 cut(s) 83, 105, 1013, 1388
HhaI GCGC 6 cut(s) 95, 167, 169, 174, 608, 1020
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 5 cut(s) 517, 1523, 1588, 1604, 1777
Hin6I GCGC 6 cut(s) 93, 165, 167, 172, 606, 1018
HinP1I GCGC 6 cut(s) 93, 165, 167, 172, 606, 1018
HincII GTYRAC 2 cut(s) 1813, 1824
HindII GTYRAC 2 cut(s) 1813, 1824
HindIII AAGCTT 1 cut(s) 316
HinfI GANTC 9 cut(s) 292, 776, 867, 921, 1034, 1266, 1703, 1841, 1858
HpaI GTTAAC 2 cut(s) 1813, 1824
HpaII CCGG 5 cut(s) 227, 237, 354, 363, 408
HphI GGTGA 3 cut(s) 397, 1210, 1883
Hpy166II GTNNAC 5 cut(s) 850, 1108, 1245, 1813, 1824
Hpy188I TCNGA 5 cut(s) 73, 781, 861, 1129, 1271
Hpy8I GTNNAC 5 cut(s) 850, 1108, 1245, 1813, 1824
Hpy99I CGWCG 5 cut(s) 121, 124, 133, 392, 1404
HpyAV CCTTC 5 cut(s) 625, 673, 1064, 1169, 1733
HpyCH4III ACNGT 2 cut(s) 40, 1242
HpyCH4IV ACGT 3 cut(s) 715, 1190, 1815
HpyF3I CTNAG 5 cut(s) 86, 573, 1128, 1532, 1732
HpySE526I ACGT 3 cut(s) 715, 1190, 1815
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 5 cut(s) 517, 1523, 1588, 1604, 1777
HspAI GCGC 6 cut(s) 93, 165, 167, 172, 606, 1018
KspAI GTTAAC 2 cut(s) 1813, 1824
KspI CCGCGG 1 cut(s) 259
Kzo9I GATC 4 cut(s) 144, 1225, 1234, 1429
LmnI GCTCC 3 cut(s) 314, 463, 1762
Lsp1109I GCAGC 6 cut(s) 156, 255, 277, 283, 385, 652
LweI GCATC 5 cut(s) 236, 802, 1111, 1479, 1825
MaeI CTAG 4 cut(s) 330, 1005, 1094, 1388
MaeII ACGT 3 cut(s) 715, 1190, 1815
MaeIII GTNAC 5 cut(s) 50, 439, 968, 1084, 1697
MalI GATC 4 cut(s) 146, 1227, 1236, 1431
MboI GATC 4 cut(s) 144, 1225, 1234, 1429
MboII GAAGA 7 cut(s) 336, 550, 854, 869, 902, 1079, 1985
MfeI CAATTG 1 cut(s) 217
MflI RGATCY 1 cut(s) 1429
MhlI GDGCHC 2 cut(s) 983, 1688
MluCI AATT 8 cut(s) 217, 325, 649, 931, 936, 1159, 1452, 1943
MlyI GAGTC 2 cut(s) 301, 1697
MmeI TCCRAC 1 cut(s) 86
Mph1103I ATGCAT 1 cut(s) 817
MseI TTAA 9 cut(s) 507, 597, 648, 666, 690, 758, 1319, 1812, 1823
MslI CAYNNNNRTG 1 cut(s) 1833
MspA1I CMGCKG 3 cut(s) 258, 268, 643
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 5 cut(s) 227, 237, 354, 363, 408
MteI GCGCNGCGC 1 cut(s) 170
MunI CAATTG 1 cut(s) 217
Mva1269I GAATGC 3 cut(s) 547, 583, 1401
MvnI CGCG 4 cut(s) 95, 167, 258, 1024
NdeI CATATG 1 cut(s) 1487
NdeII GATC 4 cut(s) 144, 1225, 1234, 1429
NlaIII CATG 5 cut(s) 517, 1523, 1588, 1604, 1777
NlaIV GGNNCC 4 cut(s) 212, 225, 241, 370
NmeAIII GCCGAG 2 cut(s) 256, 855
NmuCI GTSAC 1 cut(s) 439
NsiI ATGCAT 1 cut(s) 817
NspV TTCGAA 1 cut(s) 919
PaqCI CACCTGC 1 cut(s) 611
PauI GCGCGC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 3 cut(s) 113, 125, 128
PctI GAATGC 3 cut(s) 547, 583, 1401
PfeI GAWTC 7 cut(s) 776, 867, 921, 1034, 1266, 1841, 1858
PinAI ACCGGT 1 cut(s) 407
PkrI GCNGC 9 cut(s) 171, 260, 267, 270, 273, 302, 400, 642, 645
PleI GAGTC 2 cut(s) 300, 1697
PmaCI CACGTG 1 cut(s) 1191
PmlI CACGTG 1 cut(s) 1191
PpsI GAGTC 2 cut(s) 300, 1697
Ppu21I YACGTR 1 cut(s) 1191
PpuMI RGGWCCY 1 cut(s) 1742
PshAI GACNNNNGTC 1 cut(s) 855
PshBI ATTAAT 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 1742
PspCI CACGTG 1 cut(s) 1191
PspFI CCCAGC 3 cut(s) 371, 420, 545
PspN4I GGNNCC 4 cut(s) 212, 225, 241, 370
PspPI GGNCC 6 cut(s) 224, 368, 763, 806, 1516, 1742
PspPPI RGGWCCY 1 cut(s) 1742
PstI CTGCAG 2 cut(s) 273, 1346
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 1429
PteI GCGCGC 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 1378
RsaNI GTAC 1 cut(s) 1377
RseI CAYNNNNRTG 1 cut(s) 1833
SacII CCGCGG 1 cut(s) 259
SaqAI TTAA 9 cut(s) 507, 597, 648, 666, 690, 758, 1319, 1812, 1823
SatI GCNGC 9 cut(s) 170, 259, 266, 269, 272, 301, 399, 641, 644
Sau3AI GATC 4 cut(s) 144, 1225, 1234, 1429
Sau96I GGNCC 6 cut(s) 224, 368, 763, 806, 1516, 1742
SchI GAGTC 2 cut(s) 301, 1697
SduI GDGCHC 2 cut(s) 983, 1688
SfaNI GCATC 5 cut(s) 236, 802, 1111, 1479, 1825
SfcI CTRYAG 3 cut(s) 269, 447, 1342
Sfr303I CCGCGG 1 cut(s) 259
SfuI TTCGAA 1 cut(s) 919
SgrAI CRCCGGYG 1 cut(s) 407
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 4 cut(s) 224, 763, 1516, 1742
SmiMI CAYNNNNRTG 1 cut(s) 1833
SmlI CTYRAG 2 cut(s) 393, 665
SmoI CTYRAG 2 cut(s) 393, 665
Sse9I AATT 8 cut(s) 217, 325, 649, 931, 936, 1159, 1452, 1943
SsiI CCGC 8 cut(s) 256, 258, 301, 379, 643, 749, 1380, 1658
SspI AATATT 1 cut(s) 505
SspMI CTAG 4 cut(s) 330, 1005, 1094, 1388
TaaI ACNGT 2 cut(s) 40, 1242
TaiI ACGT 3 cut(s) 718, 1193, 1818
TaqI TCGA 6 cut(s) 143, 323, 774, 919, 1237, 1831
TasI AATT 8 cut(s) 217, 325, 649, 931, 936, 1159, 1452, 1943
TauI GCSGC 3 cut(s) 261, 303, 646
TfiI GAWTC 7 cut(s) 776, 867, 921, 1034, 1266, 1841, 1858
Tru1I TTAA 9 cut(s) 507, 597, 648, 666, 690, 758, 1319, 1812, 1823
Tru9I TTAA 9 cut(s) 507, 597, 648, 666, 690, 758, 1319, 1812, 1823
TscAI CASTG 6 cut(s) 167, 1095, 1389, 1483, 1536, 1700
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 6 cut(s) 169, 265, 268, 271, 398, 640
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 4 cut(s) 515, 551, 1149, 1474
TspGWI ACGGA 1 cut(s) 1462
TspRI CASTG 6 cut(s) 167, 1095, 1389, 1483, 1536, 1700
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 4 cut(s) 224, 763, 1516, 1742
VspI ATTAAT 1 cut(s) 507
XagI CCTNNNNNAGG 1 cut(s) 1541
XapI RAATTY 2 cut(s) 325, 931
XspI CTAG 4 cut(s) 330, 1005, 1094, 1388
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.