AT4G36660

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010021
Physical Location & Seq
Reverse (-)
17285702 .. 17287477
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G36660.1

Sequence Viewer

Length: 540 bp
ATGAAGGAGGACGATGCATTGCCGACGACGACGACGACTGGTACGGCGACGGGAACAGCTATGGCGAATTCGAAGAAGGAAAGCTCTGACTCGGTCTTGTTTGGAAGAGGTCGATATAAGTTCTGGGCGTTTGCTGCGATTTTGCTTTTGGCTTTTTGGTCGATGTTTACTGGCACCGTCACTCTCCGGTTATCTACCGGGAATCTGAACCGGTTATCGGAAGATCTAGGGATTCCGAACTACGATAATCTCGACGTTCTGGAAATGGAGGAGAGAGAGAAAGTGGTGAAGCATATGTGGGATGTGTATACTAATAGTCGTCGGATCAAATTGCCTAGATTTTGGCAAGAGGCTTTTGTTGCTGCTTATGAAGAACTCACTAGTGATGTGCCTGGGGTTAGAGAAGCTGCTATTGGTGAAATCGCCAAGATGTCGGCGCGGTCGATCACTCTTGATCCACCTCCTTCTAGATCAATGAGTGCAAGGGATTTGGGAAGAAACTTGAAGAGAATTCTACACAAGCCAGCAGCTAGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

179

Amino Acids

19.99

Weight (kDa)

8.89

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 10 - 155 3.9e-75 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 308
AccII CGCG 1 cut(s) 439
AciI CCGC 1 cut(s) 439
AclWI GGATC 2 cut(s) 332, 449
AcsI RAATTY 2 cut(s) 67, 510
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 217
AgeI ACCGGT 1 cut(s) 210
AgsI TTSAA 1 cut(s) 505
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 5 cut(s) 59, 84, 407, 530, 537
AluI AGCT 5 cut(s) 59, 84, 407, 530, 537
AlwI GGATC 2 cut(s) 332, 449
ApeKI GCWGC 4 cut(s) 134, 362, 407, 527
ApoI RAATTY 2 cut(s) 67, 510
AsiGI ACCGGT 1 cut(s) 210
AspLEI GCGC 1 cut(s) 439
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 2 cut(s) 298, 428
AsuII TTCGAA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 173
BbvI GCAGC 3 cut(s) 121, 349, 394
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 1 cut(s) 393
BcnI CCSGG 1 cut(s) 199
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 5 cut(s) 227, 336, 381, 468, 531
BglII AGATCT 1 cut(s) 223
BisI GCNGC 4 cut(s) 135, 363, 408, 528
BlsI GCNGC 4 cut(s) 136, 364, 409, 529
Bme1390I CCNGG 2 cut(s) 199, 393
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 2 cut(s) 199, 393
BmsI GCATC 1 cut(s) 4
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 199
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 2 cut(s) 186, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 43, 175
Bse3DI GCAATG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 17
BseNI ACTGG 2 cut(s) 43, 175
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 3 cut(s) 121, 349, 394
Bsh1236I CGCG 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 443
BshNI GGYRCC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 443
BsiSI CCGG 3 cut(s) 187, 198, 211
BslI CCNNNNNNNGG 1 cut(s) 217
Bsp119I TTCGAA 1 cut(s) 71
Bsp143I GATC 5 cut(s) 223, 324, 444, 454, 470
BspACI CCGC 1 cut(s) 439
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 175
BspPI GGATC 2 cut(s) 332, 449
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 43, 175
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 5 cut(s) 223, 324, 444, 454, 470
BssNAI GTATAC 1 cut(s) 309
Bst1107I GTATAC 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 2 cut(s) 100, 500
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 525
BstF5I GGATG 1 cut(s) 307
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 5 cut(s) 226, 327, 447, 457, 473
BstMBI GATC 5 cut(s) 223, 324, 444, 454, 470
BstMCI CGRYCG 1 cut(s) 443
BstMWI GCNNNNNNNGC 2 cut(s) 134, 359
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 2 cut(s) 197, 391
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 3 cut(s) 121, 349, 394
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BstZ17I GTATAC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 525
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 1 cut(s) 210
Csp6I GTAC 1 cut(s) 42
CspAI ACCGGT 1 cut(s) 210
CviJI RGCY 8 cut(s) 59, 84, 152, 353, 407, 523, 530, 537
CviKI_1 RGCY 8 cut(s) 59, 84, 152, 353, 407, 523, 530, 537
CviQI GTAC 1 cut(s) 42
DpnI GATC 5 cut(s) 225, 326, 446, 456, 472
DpnII GATC 5 cut(s) 223, 324, 444, 454, 470
Eam1104I CTCTTC 2 cut(s) 100, 500
EarI CTCTTC 2 cut(s) 100, 500
EcoRI GAATTC 2 cut(s) 67, 510
EcoRII CCWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 19
FaiI YATR 6 cut(s) 62, 117, 294, 296, 309, 369
FauNDI CATATG 1 cut(s) 294
FblI GTMKAC 1 cut(s) 308
Fnu4HI GCNGC 4 cut(s) 135, 363, 408, 528
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 4 cut(s) 135, 363, 408, 528
FspBI CTAG 5 cut(s) 227, 336, 381, 468, 531
GlaI GCGC 1 cut(s) 438
GluI GCNGC 4 cut(s) 135, 363, 408, 528
HapII CCGG 3 cut(s) 187, 198, 211
HhaI GCGC 1 cut(s) 439
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HinfI GANTC 3 cut(s) 89, 202, 232
HpaII CCGG 3 cut(s) 187, 198, 211
HphI GGTGA 2 cut(s) 298, 428
Hpy166II GTNNAC 2 cut(s) 168, 309
Hpy188I TCNGA 5 cut(s) 88, 207, 220, 237, 324
Hpy188III TCNNGA 4 cut(s) 251, 260, 452, 468
Hpy8I GTNNAC 2 cut(s) 168, 309
Hpy99I CGWCG 7 cut(s) 28, 31, 34, 37, 52, 257, 324
HpyAV CCTTC 2 cut(s) 70, 474
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 2 cut(s) 17, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 359
HpySE526I ACGT 1 cut(s) 255
HspAI GCGC 1 cut(s) 437
Kzo9I GATC 5 cut(s) 223, 324, 444, 454, 470
LpnPI CCDG 9 cut(s) 24, 109, 156, 200, 211, 224, 245, 378, 405
Lsp1109I GCAGC 3 cut(s) 121, 349, 394
LweI GCATC 1 cut(s) 4
MaeI CTAG 5 cut(s) 227, 336, 381, 468, 531
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 178
MalI GATC 5 cut(s) 225, 326, 446, 456, 472
MboI GATC 5 cut(s) 223, 324, 444, 454, 470
MboII GAAGA 6 cut(s) 85, 117, 233, 383, 507, 517
MflI RGATCY 1 cut(s) 223
MluCI AATT 3 cut(s) 67, 329, 510
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 4 cut(s) 101, 262, 343, 471
Mph1103I ATGCAT 1 cut(s) 19
MspI CCGG 3 cut(s) 187, 198, 211
MspR9I CCNGG 2 cut(s) 199, 393
MvaI CCWGG 1 cut(s) 393
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 2 cut(s) 134, 359
NciI CCSGG 1 cut(s) 199
NdeI CATATG 1 cut(s) 294
NdeII GATC 5 cut(s) 223, 324, 444, 454, 470
NlaIV GGNNCC 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 178
NsiI ATGCAT 1 cut(s) 19
NspV TTCGAA 1 cut(s) 71
PcsI WCGNNNNNNNCGW 3 cut(s) 32, 249, 440
PfeI GAWTC 2 cut(s) 202, 232
PflFI GACNNNGTC 1 cut(s) 92
PinAI ACCGGT 1 cut(s) 210
PkrI GCNGC 4 cut(s) 136, 364, 409, 529
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 175
PsuI RGATCY 1 cut(s) 223
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
SatI GCNGC 4 cut(s) 135, 363, 408, 528
Sau3AI GATC 5 cut(s) 223, 324, 444, 454, 470
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 199, 393
SetI ASST 8 cut(s) 61, 86, 112, 258, 409, 463, 532, 539
SfaNI GCATC 1 cut(s) 4
SfuI TTCGAA 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 380
Sse9I AATT 3 cut(s) 67, 329, 510
SsiI CCGC 1 cut(s) 439
SspMI CTAG 5 cut(s) 227, 336, 381, 468, 531
StyD4I CCNGG 2 cut(s) 197, 391
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 258
TaqI TCGA 5 cut(s) 71, 112, 161, 252, 443
TaqII GACCGA 1 cut(s) 82
TasI AATT 3 cut(s) 67, 329, 510
TfiI GAWTC 2 cut(s) 202, 232
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 4 cut(s) 134, 362, 407, 527
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 17, 384
Tth111I GACNNNGTC 1 cut(s) 92
XapI RAATTY 2 cut(s) 67, 510
XbaI TCTAGA 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 308
XspI CTAG 5 cut(s) 227, 336, 381, 468, 531
Zsp2I ATGCAT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.