Rroxscaffold_2G00140940

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010021
Physical Location & Seq
Forward (+)
79270389 .. 79273479
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140940.1

Sequence Viewer

Length: 537 bp
ATGAAGGATGACGATGGCCTTCCGACGACGGCGGCGGCGGGCGGCGGGAAGAGGGAGAGCTCCGATTCGAACCTAGTACTCGGGAAAGGCAGGTACAAGTTCTGGGCATTGGCGGCGATTTTGCTGCTGGCGTTCTGGTCAATGTTCACCGGCACCGTCACGCTCCGGTGGTCGGCCGGGAACCTTAATCGCTTCTCCGACGAATTTGATTCCCCTATTCACGACGATCTCGACGTCCTCGAAATGGAGGAGAGGGAGAAGGTGGTGGAGCACATGTGGGATGTGTACACAAATAGCCGTCGGATCAGATTACCCAGATTCTGGCAAGAGGCCTTTGAGGCTGCTTATGAGGACTTGACCAGTGACCTCCCTGGTGTTCGTGACTCTGCCATTTCCGAGATCGCCAAGATGTCCGTGCGGTCAGTCGATCTCGATCCACCTCCGGTCCAATCTGCTAGCGCACGGGAATTTACAAAAGCACTGAAGCATCCAGATAAAGCTAGGGAACTCTTGTCCTCAACGAAGAGTGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

178

Amino Acids

20.01

Weight (kDa)

5.29

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 9 - 148 1.8e-74 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 152
AccB7I CCANNNNNTGG 1 cut(s) 321
AciI CCGC 7 cut(s) 32, 35, 38, 42, 45, 113, 418
AclWI GGATC 2 cut(s) 311, 428
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 2 cut(s) 203, 467
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 3 cut(s) 78, 95, 287
AfiI CCNNNNNNNGG 3 cut(s) 172, 244, 321
AflIII ACRYGT 1 cut(s) 273
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 2 cut(s) 60, 500
AluI AGCT 2 cut(s) 60, 500
Alw21I GWGCWC 2 cut(s) 62, 273
AlwI GGATC 2 cut(s) 311, 428
AlwNI CAGNNNCTG 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 3 cut(s) 16, 174, 330
ApeKI GCWGC 2 cut(s) 124, 341
ApoI RAATTY 2 cut(s) 203, 467
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 445
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 1 cut(s) 139
AsuII TTCGAA 1 cut(s) 68
AsuNHI GCTAGC 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 62
Bbv12I GWGCWC 2 cut(s) 62, 273
BbvI GCAGC 2 cut(s) 111, 328
BccI CCATC 1 cut(s) 8
BceAI ACGGC 2 cut(s) 45, 282
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 372
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 3 cut(s) 74, 456, 501
BfuAI ACCTGC 1 cut(s) 81
BglI GCCNNNNNGGC 1 cut(s) 338
BisI GCNGC 6 cut(s) 33, 36, 43, 114, 125, 342
BlsI GCNGC 6 cut(s) 34, 37, 44, 115, 126, 343
BmcAI AGTACT 1 cut(s) 78
Bme1390I CCNGG 2 cut(s) 178, 372
Bme18I GGWCC 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 445
BmiI GGNNCC 2 cut(s) 154, 182
BmrFI CCNGG 2 cut(s) 178, 372
BmsI GCATC 1 cut(s) 496
BmtI GCTAGC 1 cut(s) 459
Bpu14I TTCGAA 1 cut(s) 68
BpuMI CCSGG 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 370
BsaWI WCCGGW 2 cut(s) 165, 442
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 244, 321
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 3 cut(s) 13, 286, 487
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 3 cut(s) 172, 244, 321
BseNI ACTGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 263
BseX3I CGGCCG 1 cut(s) 174
BseXI GCAGC 2 cut(s) 111, 328
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 3 cut(s) 18, 176, 332
BshNI GGYRCC 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 62, 273
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 4 cut(s) 150, 166, 177, 443
BslI CCNNNNNNNGG 3 cut(s) 172, 244, 321
BsnI GGCC 3 cut(s) 18, 176, 332
BsoBI CYCGRG 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 68
Bsp1286I GDGCHC 2 cut(s) 62, 273
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 5 cut(s) 226, 303, 399, 427, 433
BspACI CCGC 7 cut(s) 32, 35, 38, 42, 45, 113, 418
BspANI GGCC 3 cut(s) 18, 176, 332
BspLI GGNNCC 2 cut(s) 154, 182
BspMI ACCTGC 1 cut(s) 81
BspOI GCTAGC 1 cut(s) 459
BspPI GGATC 2 cut(s) 311, 428
BspT104I TTCGAA 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 5 cut(s) 226, 303, 399, 427, 433
BssNI GRCGYC 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 2 cut(s) 44, 518
BstACI GRCGYC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 3 cut(s) 40, 129, 457
BstF5I GGATG 3 cut(s) 13, 286, 487
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 5 cut(s) 229, 306, 402, 430, 436
BstMBI GATC 5 cut(s) 226, 303, 399, 427, 433
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 2 cut(s) 113, 338
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 176, 370
BstV1I GCAGC 2 cut(s) 111, 328
BstZI CGGCCG 1 cut(s) 174
BsuRI GGCC 3 cut(s) 18, 176, 332
BtsCI GGATG 3 cut(s) 13, 286, 487
BtsIMutI CAGTG 2 cut(s) 367, 479
BveI ACCTGC 1 cut(s) 81
Cac8I GCNNGC 3 cut(s) 40, 129, 457
CaiI CAGNNNCTG 1 cut(s) 321
CfoI GCGC 1 cut(s) 461
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 445
Csp6I GTAC 3 cut(s) 77, 94, 286
CviAII CATG 1 cut(s) 274
CviJI RGCY 7 cut(s) 18, 60, 176, 297, 332, 341, 500
CviKI_1 RGCY 7 cut(s) 18, 60, 176, 297, 332, 341, 500
CviQI GTAC 3 cut(s) 77, 94, 286
DpnI GATC 5 cut(s) 228, 305, 401, 429, 435
DpnII GATC 5 cut(s) 226, 303, 399, 427, 433
EaeI YGGCCR 1 cut(s) 174
EagI CGGCCG 1 cut(s) 174
Eam1104I CTCTTC 2 cut(s) 44, 518
EarI CTCTTC 2 cut(s) 44, 518
Ecl136II GAGCTC 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 445
Eco52I CGGCCG 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 62
FaeI CATG 1 cut(s) 277
FaiI YATR 2 cut(s) 275, 348
FatI CATG 1 cut(s) 273
FauI CCCGC 2 cut(s) 31, 38
Fnu4HI GCNGC 6 cut(s) 33, 36, 43, 114, 125, 342
FokI GGATG 3 cut(s) 20, 293, 474
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 6 cut(s) 33, 36, 43, 114, 125, 342
FspBI CTAG 3 cut(s) 74, 456, 501
GlaI GCGC 1 cut(s) 460
GluI GCNGC 6 cut(s) 33, 36, 43, 114, 125, 342
HaeIII GGCC 3 cut(s) 18, 176, 332
HapII CCGG 4 cut(s) 150, 166, 177, 443
HhaI GCGC 1 cut(s) 461
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HinfI GANTC 4 cut(s) 65, 209, 318, 383
HpaII CCGG 4 cut(s) 150, 166, 177, 443
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 3 cut(s) 147, 286, 288
Hpy188I TCNGA 6 cut(s) 24, 64, 199, 303, 308, 397
Hpy188III TCNNGA 6 cut(s) 82, 221, 230, 380, 431, 491
Hpy8I GTNNAC 3 cut(s) 147, 286, 288
Hpy99I CGWCG 6 cut(s) 28, 31, 203, 227, 236, 303
HpyAV CCTTC 2 cut(s) 29, 253
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 338
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 1 cut(s) 459
Kzo9I GATC 5 cut(s) 226, 303, 399, 427, 433
LmnI GCTCC 3 cut(s) 65, 168, 268
Lsp1109I GCAGC 2 cut(s) 111, 328
LweI GCATC 1 cut(s) 496
MaeI CTAG 3 cut(s) 74, 456, 501
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 3 cut(s) 157, 362, 380
MalI GATC 5 cut(s) 228, 305, 401, 429, 435
MboI GATC 5 cut(s) 226, 303, 399, 427, 433
MboII GAAGA 2 cut(s) 61, 535
MhlI GDGCHC 2 cut(s) 62, 273
MluCI AATT 2 cut(s) 203, 467
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 3 cut(s) 47, 222, 281
MseI TTAA 1 cut(s) 186
MspI CCGG 4 cut(s) 150, 166, 177, 443
MspR9I CCNGG 2 cut(s) 178, 372
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 2 cut(s) 113, 338
NciI CCSGG 1 cut(s) 178
NdeII GATC 5 cut(s) 226, 303, 399, 427, 433
NheI GCTAGC 1 cut(s) 455
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 2 cut(s) 154, 182
NmuCI GTSAC 3 cut(s) 157, 362, 380
NspI RCATGY 1 cut(s) 277
NspV TTCGAA 1 cut(s) 68
PceI AGGCCT 1 cut(s) 332
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 231, 237
PfeI GAWTC 3 cut(s) 65, 209, 318
PflMI CCANNNNNTGG 1 cut(s) 321
PkrI GCNGC 6 cut(s) 34, 37, 44, 115, 126, 343
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
PscI ACATGT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 2 cut(s) 154, 182
PspPI GGNCC 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 321
RsaI GTAC 3 cut(s) 78, 95, 287
RsaNI GTAC 3 cut(s) 77, 94, 286
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 6 cut(s) 33, 36, 43, 114, 125, 342
Sau3AI GATC 5 cut(s) 226, 303, 399, 427, 433
Sau96I GGNCC 1 cut(s) 445
ScaI AGTACT 1 cut(s) 78
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 2 cut(s) 178, 372
SduI GDGCHC 2 cut(s) 62, 273
SetI ASST 9 cut(s) 62, 75, 95, 186, 237, 264, 369, 442, 502
SfaNI GCATC 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 68
SinI GGWCC 1 cut(s) 445
Sse9I AATT 2 cut(s) 203, 467
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 7 cut(s) 32, 35, 38, 42, 45, 113, 418
SspMI CTAG 3 cut(s) 74, 456, 501
SstI GAGCTC 1 cut(s) 62
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 176, 370
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 1 cut(s) 237
TaqI TCGA 6 cut(s) 68, 231, 240, 426, 432, 532
TasI AATT 2 cut(s) 203, 467
TatI WGTACW 2 cut(s) 76, 285
TauI GCSGC 4 cut(s) 35, 38, 45, 116
TfiI GAWTC 3 cut(s) 65, 209, 318
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 2 cut(s) 367, 486
TseFI GTSAC 3 cut(s) 157, 362, 380
TseI GCWGC 2 cut(s) 124, 341
Tsp45I GTSAC 3 cut(s) 157, 362, 380
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 2 cut(s) 367, 486
Van91I CCANNNNNTGG 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 445
XapI RAATTY 2 cut(s) 203, 467
XceI RCATGY 1 cut(s) 277
XspI CTAG 3 cut(s) 74, 456, 501
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.