Prupe.7G152400_v2.0.a1

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0010021
Physical Location & Seq
Forward (+)
16289486 .. 16292789
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G152400.2

Sequence Viewer

Length: 534 bp
ATGAAGGATGAGGATGGCCTTCCAACGTCGACGACGGCCAGCGCGAAGAAGGAGAGCTCGGATTCGAGTGTAGTACTCGGGAAAGGCAGGTACAAGTTTTGGGCTTTGGCTGCGATTTTGTTGCTCGCGTTTTGGTCCATGTTCACCGGCACCGTCACGCTCCGGTGGTCCGCCGGCAACCTCAATCGGCTCTCCGACGACCTAGACTCTCCTATTCGCGACGATCTCGATGTCCTTGAAATGGAGGAGAGGGAGAAGGTGGTGAAGCACATGTGGGATGTGTACACTAACAGCCGTCGGATCAGACTGCCCAGGTTCTGGCAAGAGGCATTTGAGGCTGCCTATGAGGAGTTAACCAGTGACGTCCCTGGTGTTCGAGAAGAAGCCATAACCGAGATCGCTAAAATGTCCGTGCGCTCTGTCGATCTCGATCCGCCTCCTGTACAATCTGCAAGTGCACGGGAGTTTAGTAAGACTCTGAAGCAGGCAGAGAAAGGTAGAGAACTGGCGACCTCGACAGGGAGCGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

178

Amino Acids

19.88

Weight (kDa)

5.22

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 78
AccB1I GGYRCC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 318
AccBSI CCGCTC 1 cut(s) 525
AccI GTMKAC 1 cut(s) 29
AccII CGCG 3 cut(s) 44, 128, 219
AciI CCGC 3 cut(s) 171, 434, 525
AclWI GGATC 2 cut(s) 308, 425
AcoI YGGCCR 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 4 cut(s) 75, 92, 284, 444
AfiI CCNNNNNNNGG 3 cut(s) 241, 318, 519
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 2 cut(s) 311, 367
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw21I GWGCWC 2 cut(s) 59, 460
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 2 cut(s) 308, 425
AlwNI CAGNNNCTG 1 cut(s) 318
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 2 cut(s) 16, 36
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 2 cut(s) 110, 338
AspLEI GCGC 2 cut(s) 44, 417
AspS9I GGNCC 2 cut(s) 135, 168
AsuHPI GGTGA 2 cut(s) 136, 274
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 2 cut(s) 135, 168
BaeGI GKGCMC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 59, 460
BbvI GCAGC 2 cut(s) 97, 325
BccI CCATC 1 cut(s) 8
BceAI ACGGC 2 cut(s) 51, 279
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 2 cut(s) 313, 369
BfaI CTAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 78
BisI GCNGC 2 cut(s) 111, 339
BlsI GCNGC 2 cut(s) 112, 340
BmcAI AGTACT 1 cut(s) 75
Bme1390I CCNGG 2 cut(s) 313, 369
Bme18I GGWCC 2 cut(s) 135, 168
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 2 cut(s) 135, 168
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 313, 369
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 363
BsaJI CCNNGG 2 cut(s) 311, 367
BsaWI WCCGGW 1 cut(s) 162
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 318, 519
Bse118I RCCGGY 2 cut(s) 146, 173
Bse1I ACTGG 2 cut(s) 357, 510
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 313, 369
BseDI CCNNGG 2 cut(s) 311, 367
BseGI GGATG 3 cut(s) 13, 19, 283
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 3 cut(s) 241, 318, 519
BseNI ACTGG 2 cut(s) 357, 510
BseRI GAGGAG 2 cut(s) 260, 362
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 2 cut(s) 97, 325
Bsh1236I CGCG 3 cut(s) 44, 128, 219
Bsh1285I CGRYCG 1 cut(s) 529
BshFI GGCC 2 cut(s) 18, 38
BshNI GGYRCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 529
BsiHKAI GWGCWC 2 cut(s) 59, 460
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 3 cut(s) 147, 163, 174
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 241, 318, 519
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 2 cut(s) 18, 38
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 59, 460
Bsp1407I TGTACA 2 cut(s) 282, 442
Bsp143I GATC 5 cut(s) 223, 300, 396, 424, 430
Bsp68I TCGCGA 1 cut(s) 219
BspACI CCGC 3 cut(s) 171, 434, 525
BspANI GGCC 2 cut(s) 18, 38
BspFNI CGCG 3 cut(s) 44, 128, 219
BspLI GGNNCC 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 308, 425
BspT107I GGYRCC 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 525
BsrFI RCCGGY 2 cut(s) 146, 173
BsrGI TGTACA 2 cut(s) 282, 442
BsrI ACTGG 2 cut(s) 357, 510
BssAI RCCGGY 2 cut(s) 146, 173
BssECI CCNNGG 2 cut(s) 311, 367
BssMI GATC 5 cut(s) 223, 300, 396, 424, 430
BssNI GRCGYC 1 cut(s) 363
Bst2UI CCWGG 2 cut(s) 313, 369
Bst4CI ACNGT 1 cut(s) 154
BstACI GRCGYC 1 cut(s) 363
BstAUI TGTACA 2 cut(s) 282, 442
BstC8I GCNNGC 4 cut(s) 40, 126, 175, 486
BstF5I GGATG 3 cut(s) 13, 19, 283
BstFNI CGCG 3 cut(s) 44, 128, 219
BstHHI GCGC 2 cut(s) 44, 417
BstKTI GATC 5 cut(s) 226, 303, 399, 427, 433
BstMBI GATC 5 cut(s) 223, 300, 396, 424, 430
BstMCI CGRYCG 1 cut(s) 529
BstMWI GCNNNNNNNGC 2 cut(s) 110, 335
BstNI CCWGG 2 cut(s) 313, 369
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 311, 367
BstSLI GKGCMC 1 cut(s) 460
BstUI CGCG 3 cut(s) 44, 128, 219
BstV1I GCAGC 2 cut(s) 97, 325
BsuRI GGCC 2 cut(s) 18, 38
BtsCI GGATG 3 cut(s) 13, 19, 283
BtsIMutI CAGTG 1 cut(s) 364
BtuMI TCGCGA 1 cut(s) 219
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 4 cut(s) 40, 126, 175, 486
CaiI CAGNNNCTG 1 cut(s) 318
CfoI GCGC 2 cut(s) 44, 417
Cfr10I RCCGGY 2 cut(s) 146, 173
Cfr13I GGNCC 2 cut(s) 135, 168
Csp6I GTAC 4 cut(s) 74, 91, 283, 443
CviAII CATG 2 cut(s) 139, 271
CviJI RGCY 9 cut(s) 18, 38, 57, 104, 110, 190, 294, 338, 386
CviKI_1 RGCY 9 cut(s) 18, 38, 57, 104, 110, 190, 294, 338, 386
CviQI GTAC 4 cut(s) 74, 91, 283, 443
DpnI GATC 5 cut(s) 225, 302, 398, 426, 432
DpnII GATC 5 cut(s) 223, 300, 396, 424, 430
EaeI YGGCCR 1 cut(s) 36
EciI GGCGGA 2 cut(s) 160, 423
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 2 cut(s) 135, 168
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 500
Eco88I CYCGRG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 2 cut(s) 311, 367
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 2 cut(s) 142, 274
FaiI YATR 4 cut(s) 140, 272, 345, 389
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 138, 270
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 111, 339
FokI GGATG 3 cut(s) 20, 26, 290
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 111, 339
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 43, 416
GluI GCNGC 2 cut(s) 111, 339
HaeIII GGCC 2 cut(s) 18, 38
HapII CCGG 3 cut(s) 147, 163, 174
HhaI GCGC 2 cut(s) 44, 417
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 2 cut(s) 142, 274
Hin6I GCGC 2 cut(s) 42, 415
HinP1I GCGC 2 cut(s) 42, 415
HincII GTYRAC 2 cut(s) 30, 354
HindII GTYRAC 2 cut(s) 30, 354
HinfI GANTC 3 cut(s) 62, 206, 475
HpaI GTTAAC 1 cut(s) 354
HpaII CCGG 3 cut(s) 147, 163, 174
HphI GGTGA 2 cut(s) 136, 274
Hpy166II GTNNAC 6 cut(s) 30, 144, 283, 285, 354, 458
Hpy188I TCNGA 5 cut(s) 61, 196, 300, 305, 480
Hpy188III TCNNGA 5 cut(s) 79, 218, 227, 377, 428
Hpy8I GTNNAC 6 cut(s) 30, 144, 283, 285, 354, 458
Hpy99I CGWCG 6 cut(s) 31, 34, 37, 200, 224, 300
HpyAV CCTTC 3 cut(s) 29, 43, 250
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 2 cut(s) 26, 363
HpyCH4V TGCA 2 cut(s) 452, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 335
HpySE526I ACGT 2 cut(s) 26, 363
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 2 cut(s) 142, 274
HspAI GCGC 2 cut(s) 42, 415
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 354
Kzo9I GATC 5 cut(s) 223, 300, 396, 424, 430
LmnI GCTCC 2 cut(s) 165, 522
Lsp1109I GCAGC 2 cut(s) 97, 325
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 26, 363
MaeIII GTNAC 2 cut(s) 154, 359
MalI GATC 5 cut(s) 225, 302, 398, 426, 432
MbiI CCGCTC 1 cut(s) 525
MboI GATC 5 cut(s) 223, 300, 396, 424, 430
MboII GAAGA 2 cut(s) 58, 392
MhlI GDGCHC 2 cut(s) 59, 460
MlyI GAGTC 2 cut(s) 200, 469
MmeI TCCRAC 3 cut(s) 47, 219, 278
MnlI CCTC 9 cut(s) 4, 191, 238, 243, 319, 328, 340, 447, 523
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 1 cut(s) 353
MspI CCGG 3 cut(s) 147, 163, 174
MspR9I CCNGG 2 cut(s) 313, 369
MvaI CCWGG 2 cut(s) 313, 369
MvnI CGCG 3 cut(s) 44, 128, 219
MwoI GCNNNNNNNGC 2 cut(s) 110, 335
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 5 cut(s) 223, 300, 396, 424, 430
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 142, 274
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 154, 359
NruI TCGCGA 1 cut(s) 219
NspI RCATGY 1 cut(s) 274
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 62
PflMI CCANNNNNTGG 1 cut(s) 318
PkrI GCNGC 2 cut(s) 112, 340
PleI GAGTC 2 cut(s) 200, 469
PpsI GAGTC 2 cut(s) 200, 469
PscI ACATGT 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 311, 367
PspGI CCWGG 2 cut(s) 311, 367
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 2 cut(s) 135, 168
PstNI CAGNNNCTG 1 cut(s) 318
RruI TCGCGA 1 cut(s) 219
RsaI GTAC 4 cut(s) 75, 92, 284, 444
RsaNI GTAC 4 cut(s) 74, 91, 283, 443
SacI GAGCTC 1 cut(s) 59
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 2 cut(s) 111, 339
Sau3AI GATC 5 cut(s) 223, 300, 396, 424, 430
Sau96I GGNCC 2 cut(s) 135, 168
ScaI AGTACT 1 cut(s) 75
SchI GAGTC 2 cut(s) 200, 469
ScrFI CCNGG 2 cut(s) 313, 369
SduI GDGCHC 2 cut(s) 59, 460
SgrDI CGTCGACG 1 cut(s) 28
SinI GGWCC 2 cut(s) 135, 168
SsiI CCGC 3 cut(s) 171, 434, 525
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 2 cut(s) 311, 367
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 2 cut(s) 29, 366
TaqI TCGA 8 cut(s) 29, 65, 228, 376, 423, 429, 515, 529
TatI WGTACW 3 cut(s) 73, 282, 442
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 2 cut(s) 154, 359
TseI GCWGC 2 cut(s) 110, 338
Tsp45I GTSAC 2 cut(s) 154, 359
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 400
TspRI CASTG 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 318
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 2 cut(s) 135, 168
XceI RCATGY 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 364
ZrmI AGTACT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.