MD15G1265200.v1.1

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010021
Physical Location & Seq
Reverse (-)
22706471 .. 22709718
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1265200.v1.1.491

Sequence Viewer

Length: 537 bp
ATGAAGGATGGCGACGGCTTTCCAACAACGACTGCGGCCAGCAGCGGGAAGAAGGAGAGCTCGGATTCGGGTCTAATACTCGGGAAAGGCAGGTACAAGTTCTGGGCTTTGGCCGCGATTTTGTTGCTCGCGTTCTGGTCCATGTTCACCGGCACCGTCACGCTCCGGTGGTCCGCCGGCAACCTCAACCGCCTCTCCGACGACCTCGACTCCCGTATTCACGACGATCTCGACGTTCTTGAAATGGAAGAGAGGGAGAAGGTGGTGAAGCACATGTGGGATGTGTACACTAATAGCCGTCGGATCAGATTACCGAGGTTCTGGCAGGAAGCTTTTGAGGCTGCCTATGAGGAGTTGACAAGTGACGTCCCTGGTGTTCGAGAGGAAGCCATAACCGAGATCGCTAAGATGTCCGTCCGCTCTGTGGATCTCGATCCGCCTCCGGTGCAATCTGCAAGCGCACGAGAATTTAGTAAGACTTTGAAGCAAGCAGAGAGAGGTAGAGAACTGGTGACTTCTACAGGCAGTGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

179

Amino Acids

19.99

Weight (kDa)

5.54

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 9 - 148 3.2e-76 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 420
AccII CGCG 2 cut(s) 116, 131
AciI CCGC 7 cut(s) 35, 45, 114, 174, 190, 418, 437
AclWI GGATC 3 cut(s) 311, 428, 435
AcoI YGGCCR 2 cut(s) 36, 111
AcsI RAATTY 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 2 cut(s) 95, 287
AfiI CCNNNNNNNGG 2 cut(s) 45, 424
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 242, 484
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 2 cut(s) 60, 332
AluI AGCT 2 cut(s) 60, 332
Alw21I GWGCWC 1 cut(s) 62
AlwI GGATC 3 cut(s) 311, 428, 435
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 2 cut(s) 36, 111
ApeKI GCWGC 2 cut(s) 42, 341
ApoI RAATTY 1 cut(s) 467
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 2 cut(s) 138, 171
AsuHPI GGTGA 3 cut(s) 139, 277, 523
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 2 cut(s) 138, 171
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 62
BauI CACGAG 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 2 cut(s) 54, 328
BccI CCATC 1 cut(s) 2
BceAI ACGGC 2 cut(s) 31, 282
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 81
BisI GCNGC 4 cut(s) 36, 43, 114, 342
BlsI GCNGC 4 cut(s) 37, 44, 115, 343
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 138, 171
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 138, 171
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 372
BsaBI GATNNNNATC 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 314, 370
BsaWI WCCGGW 2 cut(s) 165, 442
BsaXI ACNNNNNCTCC 4 cut(s) 179, 194, 209, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 424
Bse118I RCCGGY 2 cut(s) 149, 176
Bse1I ACTGG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 314, 370
BseGI GGATG 2 cut(s) 13, 286
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 45, 424
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 54, 328
Bsh1236I CGCG 2 cut(s) 116, 131
BshFI GGCC 2 cut(s) 38, 113
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 4 cut(s) 150, 166, 177, 443
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 2 cut(s) 45, 424
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 2 cut(s) 38, 113
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 5 cut(s) 226, 303, 399, 427, 433
BspACI CCGC 7 cut(s) 35, 45, 114, 174, 190, 418, 437
BspANI GGCC 2 cut(s) 38, 113
BspFNI CGCG 2 cut(s) 116, 131
BspLI GGNNCC 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 81
BspPI GGATC 3 cut(s) 311, 428, 435
BspT107I GGYRCC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 420
BsrFI RCCGGY 2 cut(s) 149, 176
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 513
BssAI RCCGGY 2 cut(s) 149, 176
BssECI CCNNGG 2 cut(s) 314, 370
BssMI GATC 5 cut(s) 226, 303, 399, 427, 433
BssNI GRCGYC 1 cut(s) 366
BssSI CACGAG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 157
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 366
BstAUI TGTACA 1 cut(s) 285
BstC8I GCNNGC 5 cut(s) 40, 129, 178, 457, 489
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 13, 286
BstFNI CGCG 2 cut(s) 116, 131
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 5 cut(s) 229, 306, 402, 430, 436
BstMBI GATC 5 cut(s) 226, 303, 399, 427, 433
BstMWI GCNNNNNNNGC 3 cut(s) 113, 338, 445
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 2 cut(s) 116, 131
BstV1I GCAGC 2 cut(s) 54, 328
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 2 cut(s) 38, 113
BtsCI GGATG 2 cut(s) 13, 286
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 1 cut(s) 532
BveI ACCTGC 1 cut(s) 81
Cac8I GCNNGC 5 cut(s) 40, 129, 178, 457, 489
CfoI GCGC 1 cut(s) 461
Cfr10I RCCGGY 2 cut(s) 149, 176
Cfr13I GGNCC 2 cut(s) 138, 171
Csp6I GTAC 2 cut(s) 94, 286
CviAII CATG 2 cut(s) 142, 274
CviJI RGCY 9 cut(s) 18, 38, 60, 107, 113, 297, 332, 341, 389
CviKI_1 RGCY 9 cut(s) 18, 38, 60, 107, 113, 297, 332, 341, 389
CviQI GTAC 2 cut(s) 94, 286
DdeI CTNAG 1 cut(s) 405
DpnI GATC 5 cut(s) 228, 305, 401, 429, 435
DpnII GATC 5 cut(s) 226, 303, 399, 427, 433
EaeI YGGCCR 2 cut(s) 36, 111
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
EciI GGCGGA 2 cut(s) 163, 426
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 62
Eco47I GGWCC 2 cut(s) 138, 171
Eco53kI GAGCTC 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 62
FaeI CATG 2 cut(s) 145, 277
FaiI YATR 4 cut(s) 143, 275, 348, 392
FaqI GGGAC 1 cut(s) 353
FatI CATG 2 cut(s) 141, 273
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 4 cut(s) 36, 43, 114, 342
FokI GGATG 2 cut(s) 20, 293
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 4 cut(s) 36, 43, 114, 342
GlaI GCGC 1 cut(s) 460
GluI GCNGC 4 cut(s) 36, 43, 114, 342
HaeIII GGCC 2 cut(s) 38, 113
HapII CCGG 4 cut(s) 150, 166, 177, 443
HhaI GCGC 1 cut(s) 461
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 2 cut(s) 145, 277
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 2 cut(s) 65, 209
HpaII CCGG 4 cut(s) 150, 166, 177, 443
HphI GGTGA 3 cut(s) 139, 277, 523
Hpy166II GTNNAC 4 cut(s) 147, 286, 288, 357
Hpy188I TCNGA 4 cut(s) 64, 199, 303, 308
Hpy188III TCNNGA 6 cut(s) 82, 221, 230, 239, 380, 431
Hpy8I GTNNAC 4 cut(s) 147, 286, 288, 357
Hpy99I CGWCG 5 cut(s) 17, 203, 227, 236, 303
HpyAV CCTTC 2 cut(s) 46, 253
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 2 cut(s) 234, 366
HpyCH4V TGCA 2 cut(s) 448, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 338, 445
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 2 cut(s) 234, 366
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 2 cut(s) 145, 277
HspAI GCGC 1 cut(s) 459
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Kzo9I GATC 5 cut(s) 226, 303, 399, 427, 433
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 2 cut(s) 54, 328
MaeII ACGT 2 cut(s) 234, 366
MaeIII GTNAC 3 cut(s) 157, 362, 511
MalI GATC 5 cut(s) 228, 305, 401, 429, 435
MbiI CCGCTC 1 cut(s) 420
MboI GATC 5 cut(s) 226, 303, 399, 427, 433
MboII GAAGA 2 cut(s) 61, 260
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 1 cut(s) 467
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 3 cut(s) 47, 222, 281
MroNI GCCGGC 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 4 cut(s) 150, 166, 177, 443
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 2 cut(s) 116, 131
MwoI GCNNNNNNNGC 3 cut(s) 113, 338, 445
NaeI GCCGGC 1 cut(s) 178
NdeII GATC 5 cut(s) 226, 303, 399, 427, 433
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 2 cut(s) 145, 277
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 3 cut(s) 157, 362, 511
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 231
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 4 cut(s) 37, 44, 115, 343
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PscI ACATGT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 2 cut(s) 138, 171
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 2 cut(s) 95, 287
RsaNI GTAC 2 cut(s) 94, 286
SacI GAGCTC 1 cut(s) 62
SatI GCNGC 4 cut(s) 36, 43, 114, 342
Sau3AI GATC 5 cut(s) 226, 303, 399, 427, 433
Sau96I GGNCC 2 cut(s) 138, 171
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 62
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 2 cut(s) 138, 171
Sse9I AATT 1 cut(s) 467
SsiI CCGC 7 cut(s) 35, 45, 114, 174, 190, 418, 437
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 2 cut(s) 237, 369
TaqI TCGA 5 cut(s) 207, 231, 379, 432, 532
TasI AATT 1 cut(s) 467
TatI WGTACW 1 cut(s) 285
TauI GCSGC 2 cut(s) 38, 116
TfiI GAWTC 1 cut(s) 65
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 3 cut(s) 157, 362, 511
TseI GCWGC 2 cut(s) 42, 341
Tsp45I GTSAC 3 cut(s) 157, 362, 511
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 1 cut(s) 532
VpaK11BI GGWCC 2 cut(s) 138, 171
XapI RAATTY 1 cut(s) 467
XceI RCATGY 1 cut(s) 277
ZraI GACGTC 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.