AT5G65650

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010021
Physical Location & Seq
Forward (+)
26239413 .. 26241517
2105 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G65650.1

Sequence Viewer

Length: 558 bp
ATGAAGGATGGAGATTCCTTACCAATAAGCACTTCTTCCGTCGCGGCGACGACGGTGACCGGAAAGAAGGAAACCGGTTATTCCGCTCTCTTCTCGAAAGGCCGGTACAAGTTTTGGGCCTTAGCTGCGATTCTGTTACTCGCTTTCTGGTCAATGCTCACTGGCACGGTCAATCTCCGTTGGTCTGCCGGTAACATTAACCATTTCACCGACGATCTCGTCTTTCCCATCCACGAGGATCTCGATGTTCTTGAAATGGAGGAGAGGGAGAAAGTGGTGAAGCACATGTGGGATGTGTATAACAATGGCCGTCGTATCAGATTACCTAGGTTTTGGCAAGAGGCCTTCGAGGCTGCTTACGAGGAGCTTACCAGTGATGTACCTGATGTGGTTGAGGCTGCCATTTCCGAGATCGCTAGGATGTCTATTCGCTCCATTGTTATAGATCCGCCTCCGCTTCATTCCACGAATGTACGAGAATTGACAAAGACTCTAAAACTTGCGGACAAAGGAAGAGCAACTCAAATATCAAAACCAAGCAGCCGTCGATTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

185

Amino Acids

20.94

Weight (kDa)

7.96

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 8 - 152 4.2e-77 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccBSI CCGCTC 1 cut(s) 86
AccII CGCG 1 cut(s) 44
AciI CCGC 5 cut(s) 44, 84, 449, 455, 503
AclWI GGATC 2 cut(s) 246, 440
AcoI YGGCCR 1 cut(s) 307
AfaI GTAC 3 cut(s) 107, 381, 474
AflIII ACRYGT 1 cut(s) 285
AgeI ACCGGT 1 cut(s) 74
AgsI TTSAA 2 cut(s) 254, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 16, 48
AluBI AGCT 2 cut(s) 125, 367
AluI AGCT 2 cut(s) 125, 367
AlwI GGATC 2 cut(s) 246, 440
AoxI GGCC 4 cut(s) 100, 117, 307, 342
ApeKI GCWGC 4 cut(s) 125, 353, 398, 540
AsiGI ACCGGT 1 cut(s) 74
AspA2I CCTAGG 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 67, 199, 289
AvrII CCTAGG 1 cut(s) 326
BauI CACGAG 1 cut(s) 233
BbvI GCAGC 4 cut(s) 112, 340, 385, 552
BccI CCATC 2 cut(s) 2, 236
BceAI ACGGC 2 cut(s) 294, 528
BfaI CTAG 2 cut(s) 327, 417
BglI GCCNNNNNGGC 1 cut(s) 350
BisI GCNGC 5 cut(s) 45, 126, 354, 399, 541
BlnI CCTAGG 1 cut(s) 326
BlsI GCNGC 5 cut(s) 46, 127, 355, 400, 542
BmgT120I GGNCC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 326
BsaWI WCCGGW 2 cut(s) 59, 74
Bse118I RCCGGY 3 cut(s) 74, 102, 188
Bse1I ACTGG 2 cut(s) 166, 372
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 4 cut(s) 13, 228, 298, 426
BseNI ACTGG 2 cut(s) 166, 372
BseRI GAGGAG 2 cut(s) 275, 377
BseXI GCAGC 4 cut(s) 112, 340, 385, 552
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 4 cut(s) 102, 119, 309, 344
BshTI ACCGGT 1 cut(s) 74
BsiSI CCGG 4 cut(s) 60, 75, 103, 189
BsnI GGCC 4 cut(s) 102, 119, 309, 344
Bsp143I GATC 4 cut(s) 214, 238, 411, 445
BspACI CCGC 5 cut(s) 44, 84, 449, 455, 503
BspANI GGCC 4 cut(s) 102, 119, 309, 344
BspFNI CGCG 1 cut(s) 44
BspPI GGATC 2 cut(s) 246, 440
BspQI GCTCTTC 1 cut(s) 508
BsrBI CCGCTC 1 cut(s) 86
BsrFI RCCGGY 3 cut(s) 74, 102, 188
BsrI ACTGG 2 cut(s) 166, 372
BssAI RCCGGY 3 cut(s) 74, 102, 188
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 4 cut(s) 214, 238, 411, 445
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 326
Bst2BI CACGAG 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 55, 169
Bst6I CTCTTC 2 cut(s) 95, 508
BstDEI CTNAG 1 cut(s) 121
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 4 cut(s) 13, 228, 298, 426
BstFNI CGCG 1 cut(s) 44
BstKTI GATC 4 cut(s) 217, 241, 414, 448
BstMBI GATC 4 cut(s) 214, 238, 411, 445
BstMWI GCNNNNNNNGC 2 cut(s) 125, 350
BstNSI RCATGY 1 cut(s) 289
BstPI GGTNACC 1 cut(s) 55
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 4 cut(s) 112, 340, 385, 552
BstX2I RGATCY 2 cut(s) 238, 445
BstYI RGATCY 2 cut(s) 238, 445
BsuRI GGCC 4 cut(s) 102, 119, 309, 344
BtsCI GGATG 4 cut(s) 13, 228, 298, 426
BtsIMutI CAGTG 2 cut(s) 159, 379
Cfr10I RCCGGY 3 cut(s) 74, 102, 188
Cfr13I GGNCC 1 cut(s) 117
Csp6I GTAC 3 cut(s) 106, 380, 473
CspAI ACCGGT 1 cut(s) 74
CviAII CATG 1 cut(s) 286
CviJI RGCY 9 cut(s) 102, 119, 125, 309, 344, 353, 367, 398, 543
CviKI_1 RGCY 9 cut(s) 102, 119, 125, 309, 344, 353, 367, 398, 543
CviQI GTAC 3 cut(s) 106, 380, 473
DdeI CTNAG 1 cut(s) 121
DpnI GATC 4 cut(s) 216, 240, 413, 447
DpnII GATC 4 cut(s) 214, 238, 411, 445
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 95, 508
EarI CTCTTC 2 cut(s) 95, 508
EciI GGCGGA 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 326
Eco147I AGGCCT 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 55
EcoO65I GGTNACC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 1 cut(s) 289
FaiI YATR 3 cut(s) 287, 300, 443
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FatI CATG 1 cut(s) 285
Fnu4HI GCNGC 5 cut(s) 45, 126, 354, 399, 541
FokI GGATG 4 cut(s) 20, 215, 305, 433
Fsp4HI GCNGC 5 cut(s) 45, 126, 354, 399, 541
FspBI CTAG 2 cut(s) 327, 417
GluI GCNGC 5 cut(s) 45, 126, 354, 399, 541
HaeIII GGCC 4 cut(s) 102, 119, 309, 344
HapII CCGG 4 cut(s) 60, 75, 103, 189
Hin1II CATG 1 cut(s) 289
HinfI GANTC 3 cut(s) 14, 130, 490
HpaII CCGG 4 cut(s) 60, 75, 103, 189
HphI GGTGA 3 cut(s) 67, 199, 289
Hpy188I TCNGA 2 cut(s) 320, 409
Hpy188III TCNNGA 3 cut(s) 94, 242, 251
Hpy99I CGWCG 6 cut(s) 44, 52, 55, 215, 315, 549
HpyAV CCTTC 2 cut(s) 61, 355
HpyCH4III ACNGT 2 cut(s) 55, 169
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 350
HpyF3I CTNAG 1 cut(s) 121
Hsp92II CATG 1 cut(s) 289
Kzo9I GATC 4 cut(s) 214, 238, 411, 445
LguI GCTCTTC 1 cut(s) 508
LmnI GCTCC 2 cut(s) 364, 437
LpnPI CCDG 8 cut(s) 73, 88, 116, 133, 147, 202, 385, 396
Lsp1109I GCAGC 4 cut(s) 112, 340, 385, 552
MaeI CTAG 2 cut(s) 327, 417
MaeIII GTNAC 3 cut(s) 55, 135, 191
MalI GATC 4 cut(s) 216, 240, 413, 447
MbiI CCGCTC 1 cut(s) 86
MboI GATC 4 cut(s) 214, 238, 411, 445
MboII GAAGA 3 cut(s) 27, 82, 525
MflI RGATCY 2 cut(s) 238, 445
MluCI AATT 1 cut(s) 479
MlyI GAGTC 1 cut(s) 484
MnlI CCTC 8 cut(s) 229, 253, 258, 334, 343, 355, 388, 462
MseI TTAA 1 cut(s) 198
MspI CCGG 4 cut(s) 60, 75, 103, 189
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 2 cut(s) 125, 350
NdeII GATC 4 cut(s) 214, 238, 411, 445
NlaIII CATG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 289
PceI AGGCCT 1 cut(s) 344
PciI ACATGT 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 2 cut(s) 14, 130
PinAI ACCGGT 1 cut(s) 74
PkrI GCNGC 5 cut(s) 46, 127, 355, 400, 542
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PscI ACATGT 1 cut(s) 285
PspEI GGTNACC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 2 cut(s) 238, 445
RsaI GTAC 3 cut(s) 107, 381, 474
RsaNI GTAC 3 cut(s) 106, 380, 473
SapI GCTCTTC 1 cut(s) 508
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 5 cut(s) 45, 126, 354, 399, 541
Sau3AI GATC 4 cut(s) 214, 238, 411, 445
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 1 cut(s) 484
SetI ASST 5 cut(s) 127, 328, 332, 369, 385
Sse9I AATT 1 cut(s) 479
SseBI AGGCCT 1 cut(s) 344
SsiI CCGC 5 cut(s) 44, 84, 449, 455, 503
SspMI CTAG 2 cut(s) 327, 417
StuI AGGCCT 1 cut(s) 344
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 2 cut(s) 55, 169
TaqI TCGA 4 cut(s) 95, 243, 348, 547
TasI AATT 1 cut(s) 479
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 2 cut(s) 14, 130
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 2 cut(s) 166, 379
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 4 cut(s) 125, 353, 398, 540
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 17, 449
TspGWI ACGGA 2 cut(s) 28, 167
TspRI CASTG 2 cut(s) 166, 379
XceI RCATGY 1 cut(s) 289
XmaJI CCTAGG 1 cut(s) 326
XspI CTAG 2 cut(s) 327, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.