pycom15g23100

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010021
Physical Location & Seq
Reverse (-)
17145590 .. 17149167
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g23100.1

Sequence Viewer

Length: 543 bp
ATGAAGGATGGCGACGGCTTTCCAACAACGACTGCGGCCAGCAGCGGGAAGAAGGAGAGCTCGGATTCGGGTCTAATACTCGGGAAAGGCAGGTACAAGTTCTGGGCTTTGGCCGCGATTTTGTTGCTCGCGTTTTGGTCCATGTTCACCGGCACCGTCACGCTCCGGTGGTCCGCCGGCAACCTCAACCGCCTCTCCGACGACCTCAACTCCCCCATTCACGACGATCTCGACGTTCTTGAAATGGAAGGGAGGGAGAAGGTGGTGAAGCACATGTGGGATGTGTACACCAATAGCCGTCGGATCAGATTACCGAGGTTCTGGCAGGAAGCTTTTGAGGCTGCCTATGAGGAGTTGACAAGTGACGTCCCTGGTGTTCGAGAGGAAGCCATAACCGAGATCGCTAAGATGTCCGTCCGCTCTGTCTCTGTGGATCTCGATCCGCCTCCGGTGCAATCTGCAAGCGCACGAGAATTTAGTAAGACTTTGAAGCAAGCAGAGAGAGGTAGAGAACTGGTGACTTCTACGGGCAGTGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

181

Amino Acids

20.04

Weight (kDa)

5.79

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 9 - 146 1.2e-74 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 420
AccII CGCG 2 cut(s) 116, 131
AciI CCGC 7 cut(s) 35, 45, 114, 174, 190, 418, 443
AclWI GGATC 3 cut(s) 311, 434, 441
AcoI YGGCCR 2 cut(s) 36, 111
AcsI RAATTY 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 2 cut(s) 95, 287
AfiI CCNNNNNNNGG 1 cut(s) 45
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 242, 490
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 2 cut(s) 60, 332
AluI AGCT 2 cut(s) 60, 332
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 3 cut(s) 311, 434, 441
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 2 cut(s) 36, 111
ApeKI GCWGC 2 cut(s) 42, 341
ApoI RAATTY 1 cut(s) 473
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 2 cut(s) 138, 171
AsuHPI GGTGA 3 cut(s) 139, 277, 529
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 2 cut(s) 138, 171
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 62
BauI CACGAG 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 2 cut(s) 54, 328
BccI CCATC 1 cut(s) 2
BceAI ACGGC 2 cut(s) 31, 282
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 430
BfuAI ACCTGC 1 cut(s) 81
BisI GCNGC 4 cut(s) 36, 43, 114, 342
BlsI GCNGC 4 cut(s) 37, 44, 115, 343
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 138, 171
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 2 cut(s) 138, 171
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 372
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 314, 370
BsaWI WCCGGW 2 cut(s) 165, 448
BsaXI ACNNNNNCTCC 4 cut(s) 179, 194, 209, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse118I RCCGGY 2 cut(s) 149, 176
Bse1I ACTGG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 314, 370
BseGI GGATG 2 cut(s) 13, 286
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 54, 328
Bsh1236I CGCG 2 cut(s) 116, 131
BshFI GGCC 2 cut(s) 38, 113
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 4 cut(s) 150, 166, 177, 449
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 353
BsnI GGCC 2 cut(s) 38, 113
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 5 cut(s) 226, 303, 399, 433, 439
BspACI CCGC 7 cut(s) 35, 45, 114, 174, 190, 418, 443
BspANI GGCC 2 cut(s) 38, 113
BspFNI CGCG 2 cut(s) 116, 131
BspLI GGNNCC 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 81
BspPI GGATC 3 cut(s) 311, 434, 441
BspT107I GGYRCC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 420
BsrFI RCCGGY 2 cut(s) 149, 176
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 519
BssAI RCCGGY 2 cut(s) 149, 176
BssECI CCNNGG 2 cut(s) 314, 370
BssMI GATC 5 cut(s) 226, 303, 399, 433, 439
BssNI GRCGYC 1 cut(s) 366
BssSI CACGAG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 157
BstACI GRCGYC 1 cut(s) 366
BstAUI TGTACA 1 cut(s) 285
BstC8I GCNNGC 5 cut(s) 40, 129, 178, 463, 495
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 13, 286
BstFNI CGCG 2 cut(s) 116, 131
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 5 cut(s) 229, 306, 402, 436, 442
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 5 cut(s) 226, 303, 399, 433, 439
BstMWI GCNNNNNNNGC 3 cut(s) 113, 338, 451
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 370
BstUI CGCG 2 cut(s) 116, 131
BstV1I GCAGC 2 cut(s) 54, 328
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 2 cut(s) 38, 113
BtsCI GGATG 2 cut(s) 13, 286
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 538
BveI ACCTGC 1 cut(s) 81
Cac8I GCNNGC 5 cut(s) 40, 129, 178, 463, 495
CfoI GCGC 1 cut(s) 467
Cfr10I RCCGGY 2 cut(s) 149, 176
Cfr13I GGNCC 2 cut(s) 138, 171
Csp6I GTAC 2 cut(s) 94, 286
CviAII CATG 2 cut(s) 142, 274
CviJI RGCY 9 cut(s) 18, 38, 60, 107, 113, 297, 332, 341, 389
CviKI_1 RGCY 9 cut(s) 18, 38, 60, 107, 113, 297, 332, 341, 389
CviQI GTAC 2 cut(s) 94, 286
DdeI CTNAG 1 cut(s) 405
DpnI GATC 5 cut(s) 228, 305, 401, 435, 441
DpnII GATC 5 cut(s) 226, 303, 399, 433, 439
EaeI YGGCCR 2 cut(s) 36, 111
EciI GGCGGA 2 cut(s) 163, 432
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 62
Eco47I GGWCC 2 cut(s) 138, 171
Eco53kI GAGCTC 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 62
FaeI CATG 2 cut(s) 145, 277
FaiI YATR 4 cut(s) 143, 275, 348, 392
FaqI GGGAC 1 cut(s) 353
FatI CATG 2 cut(s) 141, 273
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 4 cut(s) 36, 43, 114, 342
FokI GGATG 2 cut(s) 20, 293
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 4 cut(s) 36, 43, 114, 342
GlaI GCGC 1 cut(s) 466
GluI GCNGC 4 cut(s) 36, 43, 114, 342
HaeIII GGCC 2 cut(s) 38, 113
HapII CCGG 4 cut(s) 150, 166, 177, 449
HhaI GCGC 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 2 cut(s) 145, 277
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 1 cut(s) 65
HpaII CCGG 4 cut(s) 150, 166, 177, 449
HphI GGTGA 3 cut(s) 139, 277, 529
Hpy166II GTNNAC 4 cut(s) 147, 286, 288, 357
Hpy188I TCNGA 4 cut(s) 64, 199, 303, 308
Hpy188III TCNNGA 6 cut(s) 82, 221, 230, 239, 380, 437
Hpy8I GTNNAC 4 cut(s) 147, 286, 288, 357
Hpy99I CGWCG 5 cut(s) 17, 203, 227, 236, 303
HpyAV CCTTC 3 cut(s) 46, 242, 253
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 2 cut(s) 234, 366
HpyCH4V TGCA 2 cut(s) 454, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 338, 451
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 2 cut(s) 234, 366
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 2 cut(s) 145, 277
HspAI GCGC 1 cut(s) 465
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Kzo9I GATC 5 cut(s) 226, 303, 399, 433, 439
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 2 cut(s) 54, 328
MaeII ACGT 2 cut(s) 234, 366
MaeIII GTNAC 3 cut(s) 157, 362, 517
MalI GATC 5 cut(s) 228, 305, 401, 435, 441
MbiI CCGCTC 1 cut(s) 420
MboI GATC 5 cut(s) 226, 303, 399, 433, 439
MboII GAAGA 1 cut(s) 61
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 62
MluCI AATT 1 cut(s) 473
MmeI TCCRAC 3 cut(s) 47, 222, 281
MroNI GCCGGC 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 4 cut(s) 150, 166, 177, 449
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 2 cut(s) 116, 131
MwoI GCNNNNNNNGC 3 cut(s) 113, 338, 451
NaeI GCCGGC 1 cut(s) 178
NdeII GATC 5 cut(s) 226, 303, 399, 433, 439
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 2 cut(s) 145, 277
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 3 cut(s) 157, 362, 517
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 231
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 4 cut(s) 37, 44, 115, 343
PscI ACATGT 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 2 cut(s) 138, 171
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 2 cut(s) 95, 287
RsaNI GTAC 2 cut(s) 94, 286
SacI GAGCTC 1 cut(s) 62
SatI GCNGC 4 cut(s) 36, 43, 114, 342
Sau3AI GATC 5 cut(s) 226, 303, 399, 433, 439
Sau96I GGNCC 2 cut(s) 138, 171
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 62
SinI GGWCC 2 cut(s) 138, 171
Sse9I AATT 1 cut(s) 473
SsiI CCGC 7 cut(s) 35, 45, 114, 174, 190, 418, 443
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 2 cut(s) 237, 369
TaqI TCGA 4 cut(s) 231, 379, 438, 538
TasI AATT 1 cut(s) 473
TatI WGTACW 1 cut(s) 285
TauI GCSGC 2 cut(s) 38, 116
TfiI GAWTC 1 cut(s) 65
TscAI CASTG 1 cut(s) 538
TseFI GTSAC 3 cut(s) 157, 362, 517
TseI GCWGC 2 cut(s) 42, 341
Tsp45I GTSAC 3 cut(s) 157, 362, 517
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 1 cut(s) 538
VpaK11BI GGWCC 2 cut(s) 138, 171
XapI RAATTY 1 cut(s) 473
XceI RCATGY 1 cut(s) 277
ZraI GACGTC 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.