AT4G39850

ABC transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006235
Physical Location & Seq
Forward (+)
18488434 .. 18497266
8833 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G39850.4

Sequence Viewer

Length: 4014 bp
ATGCCTTCACTTCAACTATTGCAGTTAACTGAGCGGGGTCGGGGTCTTGTAGCGTCAAGACGGAAATCTATACTGCTTGCGGCTGGGATTGTAGCTGCTGGTGGAACTGCTGTTTACCTGAAATCAAGGGTCGCTTCCCGGAGGCCTGATTCTTCGCGTCTTTGCAATGGTCAGAGTGATGATGATGAGACTTTGGAAAAGCTGACTGCAACTGATCAAAATGCAAAGATAACCACGAAAAAGAAGAAAGGAGGAGGATTGAAGTCTCTTCAGGTTCTGACTGCTATTCTTCTCTCTCAGATGGGAAAAATGGGTGCCAGGGATCTTTTGGCACTAGTCGCCACCGTGGTTTTCAGAACAGCTTTGAGCAATAGATTGGCAAAAGTGCAAGGTTTCCTTTTCCGTGCTGCTTTCTTAAGGCGTGCGCCACTGTTTCTACGGCTCATCTCCGAGAATATTATGTTGTGTTTCATGCTATCAACATTGCACTCTACTTCAAAGTACATAACTGGGGCATTGAGTTTGCGATTCAGAAAGATATTGACCAAGATTATCCATTCACACTATTTTGAGAATATGGTATATTACAAAATATCACACGTGGATGGTCGGATTACGCACCCTGAACAAAGAATTGCCAGCGATGTACCAAGATTCTCCTCAGAGTTGAGCGATCTTATACTGGATGATTTGACGGCGGTTACTGATGGAATTTTGTATGCATGGCGCCTGTGTTCATATGCTAGTCCAAAATACATCTTCTGGATACTGGCCTATGTACTGGGGGCTGGGACGGCGATAAGAAACTTTTCTCCTTCTTTTGGGAAATTGATGTCCAAGGAACAGCAGTTAGAAGGAGAGTACCGGCAACTTCATTCACGCTTAAGGACTCATTCGGAAAGCATAGCATTCTATGGTGGGGAAACCAGGGAAGAATCTCATATACAACAAAAGTTCAAGAATCTTGTTAGCCATATGAGTCACGTGCTTCATGATCACTGGTGGTTTGGTATGATCCAAGATTTTCTGCTGAAGTATCTTGGGGCCACAGTTGCAGTTATTCTGATTATCGAACCATTCTTCTCTGGGCATCTAAGACCTGACGACTCGACCTTAGGAAGAGCTGAGATGCTTAGCAATATAAGATATCACACTAGTGTCATTATATCTCTCTTTCAGGCGTTGGGAACACTTTCTATAAGTTCCAGGCGGCTCAACCGACTCAGTGGTTATGCTGACCGAATCCATGAGTTGATGGCTGTCTCAAGAGAACTCAGTGGTGATGATAAATCGTCTTTCCAGAGAAATAGAAGCAGAAATTATCTAAGTGAAGCTAATTATGTAGAGTTTTCCGATGTCAAGGTTGTTACTCCAACCGGAAATGTTTTGGTGGAGGATCTCACCCTTCGAGTTGAGCAAGGGTCTAATCTTCTGATTACAGGTCCTAATGGAAGTGGCAAGAGTTCCCTTTTCCGAGTATTAGGAGGTCTATGGCCCCTGGTGTCTGGACATATTGTGAAGCCAGGAGTTGGTTCTGATCTTAACAAGGAGATCTTCTATGTGCCGCAACGGCCTTATATGGCAGTAGGAACACTTCGTGACCAGTTAATATATCCTCTTACTTCTGGCCAAGAGAGTGAACTGCTCACTGAGATTGGAATGGTGGAGCTATTGAAAAATGTTGATCTAGAATATTTATTGGATCGCTACCAACCTGAAAAAGAGGTTAATTGGGGTGATGAATTATCTCTTGGAGAGCAACAGAGATTGGGTATGGCCAGACTATTCTACCACAAACCCAAATTTGCAATTCTAGATGAATGCACAAGTGCTGTCACAACTGATATGGAAGAACGCTTTGCCGCTAAGGTTCGAGCTATGGGAACTTCTTGCATAACAATCTCCCATCGTCCAGCGCTTGTTGCATTCCATGATGTTGTTCTGTCATTAGACGGTGAAGGAGGATGGAGTGTTCATTACAAGAGGGATGACTCTGCCCTTCTGACGGATGCTGAAATTGATTCAGTGAAAAGTTCAGATACAGATCGGCAAAATGATGCGATGGTTGTTCAACGAGCGTTTGCTGCAGCTAGAAAGGAATCTGCTACTAATTCAAAGGCTCAGTCGTACCAGACACAGTTAATTGCAAGATCACCTGTTGTAGATAAAAGTGTAGTGTTGCCTCGTTTTCCTCAACCTCAAACATCCCAAAGGGCTTTACCATCAAGAGTAGCTGCAATGTTAAACGTGTTGATACCCACTATATTTGACAAGCAAGGAGCTCAACTGCTTGCTGTTGCTTGCCTTGTCGTCTCAAGAACGCTGATCTCTGACCGAATAGCCTCTTTGAATGGGACCACTGTGAAGTATGTCTTGGAGCAAGATAAGGCAGCCTTTGTTCGTTTGATTGGTTTGAGTGTTCTCCAAAGTGGTGCATCTTCTATAATTGCTCCTTCACTAAGGCATTTAACGCAAAGGCTAGCGTTAGGGTGGAGGATTCGTTTGACTCAACATCTGCTAAGGAACTATTTGAGAAATAATGCGTTTTACAAGGTTTTCCACATGTCAGGCAATAGTATTGATGCGGACCAGAGACTCACTCGTGACCTGGAAAAGTTAACCGCTGACTTGTCTGGACTTCTTACTGGAATGGTAAAGCCATCGGTTGACATTCTCTGGTTCACCTGGAGGATGAAGTTACTGACTGGTCAGAGGGGAGTTGCCATACTTTACACATATATGTTACTTGGTCTTGGTTTTCTGAGACGTGTTGCTCCCGATTTCGGTGATCTAGCCGGTGAAGAACAGCAGCTTGAAGGGAAGTTTCGGTTTATGCACGAGAGGCTGAACACTCATGCTGAATCTATTGCATTCTTTGGAGGTGGAGCTCGAGAAAAGGCTATGGTTGACAAAAAATTCAGGGCCCTACTGGATCATTCTCTCATGCTCTTGAGGAAGAAATGGTTGTATGGCATACTTGATGATTTTGTGACAAAGCAACTTCCCAATAATGTGACGTGGGGATTGAGTTTATTGTATGCCCTAGAACACAAGGGAGACAGAGCACTTGTCTCCACTCAAGGTGAATTGGCACATGCATTGCGGTATCTAGCTTCTGTTGTCTCCCAAAGCTTTATGGCTTTTGGCGATATTCTTGAACTACACAAGAAGTTCCTGGAGCTCTCTGGTGGTATTAACAGAATTTTTGAGCTCGATGAGTTTTTGGATGCTTCTCAGTCAGGTGTTACCTCAGAAAATCAAACAAGTCGTTTGGATTCTCAAGATCTACTTTCCTTTTCGGAGGTGGATATCATTACCCCTGCTCAGAAATTGATGGCTAGCAAGTTGTCGTGTGAAATAGTTTCAGGGAAAAGCCTGCTCGTCACAGGTCCAAATGGTAGTGGAAAGACTTCAGTATTTAGAGTCCTTAGAGATATCTGGCCCACTGTATGTGGAAGACTTACCAAACCATCATTGGATATCAAAGAACTTGGGTCAGGGAATGGCATGTTTTTTGTCCCGCAGCGACCTTATACATGTTTAGGGACACTGAGAGATCAAATTATATACCCTCTATCTAAAGAAGAAGCAGAGAAAAGGGCAGCAAAGTTGTACACCAGTGGAGAGAGCTCAACAGAAGCTGGAAGCATTCTGGATTCTCATTTGAAAACCATTCTGGAGAATGTTCGGTTAGTTTATCTCTTGGAAAGAGACGTAGGTGGTTGGGATGCTACTACCAATTGGGAAGACATATTATCTCTTGGAGAGCAACAGAGATTAGGCATGGCACGTTTATTCTTTCACAGGCCGAAGTTTGGAGTCCTTGATGAATGCACAAATGCGACGAGTGTTGATGTTGAGGAACAGCTCTATAGAGTTGCACGAGACATGGGAGTCACTTTCATAACCTCATCACAACGGCCGGCTCTGATCCCATTCCATTCCTTGGAGCTAAGGCTGATTGATGGAGAAGGAAACTGGGAGCTCCGTTCGATCGAACAGACAACAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006629 GO:0008150 GO:0008152 GO:0009056 GO:0016042 GO:0044238 GO:0071704 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko02010 ko04146 map02010 map04146
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

1337

Amino Acids

149.57

Weight (kDa)

9.03

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 93 - 361 1.3e-89 ABC transporter transmembrane region 2
ABC_tran PF00005 465 - 610 1e-18 ABC transporter
ABC_membrane_2 PF06472 747 - 1013 2.7e-86 ABC transporter transmembrane region 2
ABC_tran PF00005 1115 - 1280 4.7e-11 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3893
AccB1I GGYRCC 2 cut(s) 314, 726
AccB7I CCANNNNNTGG 2 cut(s) 1529, 3946
AccBSI CCGCTC 1 cut(s) 34
AccII CGCG 1 cut(s) 157
AclWI GGATC 6 cut(s) 330, 1009, 1404, 1710, 2940, 3925
AcoI YGGCCR 3 cut(s) 1627, 1776, 3920
AcsI RAATTY 4 cut(s) 711, 1802, 2915, 3201
AcuI CTGAAG 3 cut(s) 254, 1052, 3396
AcvI CACGTG 2 cut(s) 601, 985
AcyI GRCGYC 1 cut(s) 727
AdeI CACNNNGTG 1 cut(s) 1598
AfaI GTAC 6 cut(s) 503, 648, 780, 863, 2129, 3614
AfeI AGCGCT 1 cut(s) 1917
AfiI CCNNNNNNNGG 6 cut(s) 821, 1529, 2005, 2783, 3815, 3946
AflII CTTAAG 2 cut(s) 415, 883
AflIII ACRYGT 5 cut(s) 598, 2247, 2562, 2767, 3536
AhlI ACTAGT 2 cut(s) 334, 1154
AjiI CACGTC 2 cut(s) 2768, 3018
AjnI CCWGG 8 cut(s) 317, 926, 1205, 1497, 1522, 2607, 2684, 3174
AjuI GAANNNNNNNTTGG 4 cut(s) 1734, 1766, 2357, 2389
AleI CACNNNNGTG 1 cut(s) 1155
AloI GAACNNNNNNTCC 2 cut(s) 1956, 1988
Alw21I GWGCWC 7 cut(s) 2284, 2890, 3067, 3183, 3213, 3632, 3987
AlwI GGATC 6 cut(s) 330, 1009, 1404, 1710, 2940, 3925
AlwNI CAGNNNCTG 2 cut(s) 277, 3641
Ama87I CYCGRG 1 cut(s) 2889
Aor51HI AGCGCT 1 cut(s) 1917
ApaI GGGCCC 1 cut(s) 2926
ApeKI GCWGC 9 cut(s) 95, 407, 2084, 2087, 2234, 2390, 2809, 3523, 3602
ApoI RAATTY 4 cut(s) 711, 1802, 2915, 3201
ArsI GACNNNNNNTTYG 2 cut(s) 2108, 2140
Asp700I GAANNNNTTC 3 cut(s) 808, 1192, 3409
AspLEI GCGC 3 cut(s) 427, 729, 1918
AspS9I GGNCC 9 cut(s) 1044, 1442, 1494, 2355, 2587, 2922, 2923, 3389, 3441
AsuC2I CCSGG 1 cut(s) 139
AsuHPI GGTGA 9 cut(s) 1292, 1393, 1748, 1967, 2145, 2674, 2798, 2810, 3095
AsuNHI GCTAGC 2 cut(s) 2479, 3338
AvaI CYCGRG 1 cut(s) 2889
AvaII GGWCC 4 cut(s) 1442, 2355, 2587, 3389
AxyI CCTNAGG 1 cut(s) 1114
BaeGI GKGCMC 1 cut(s) 2926
BalI TGGCCA 2 cut(s) 1629, 1778
BanI GGYRCC 2 cut(s) 314, 726
BanII GRGCYC 7 cut(s) 2284, 2890, 2926, 3183, 3213, 3632, 3987
BarI GAAGNNNNNNTAC 2 cut(s) 1578, 1610
BauI CACGAG 3 cut(s) 2601, 2837, 3882
BbrPI CACGTG 2 cut(s) 601, 985
BbsI GAAGAC 2 cut(s) 3463, 3753
Bbv12I GWGCWC 7 cut(s) 2284, 2890, 3067, 3183, 3213, 3632, 3987
BbvI GCAGC 9 cut(s) 82, 394, 2071, 2099, 2221, 2402, 2821, 3535, 3614
BceAI ACGGC 5 cut(s) 455, 711, 810, 1586, 3935
BciT130I CCWGG 8 cut(s) 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
BciVI GTATCC 1 cut(s) 759
BclI TGATCA 2 cut(s) 214, 994
BcnI CCSGG 1 cut(s) 139
BcuI ACTAGT 2 cut(s) 334, 1154
BfmI CTRYAG 2 cut(s) 2085, 3871
BfoI RGCGCY 2 cut(s) 730, 1919
BfrI CTTAAG 2 cut(s) 415, 883
BfuI GTATCC 1 cut(s) 759
BglI GCCNNNNNGGC 1 cut(s) 1570
BglII AGATCT 2 cut(s) 1551, 3283
BlpI GCTNAGC 1 cut(s) 1133
Bme1390I CCNGG 9 cut(s) 139, 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
Bme18I GGWCC 4 cut(s) 1442, 2355, 2587, 3389
BmeT110I CYCGRG 1 cut(s) 2889
BmgBI CACGTC 2 cut(s) 2768, 3018
BmgT120I GGNCC 9 cut(s) 1044, 1442, 1494, 2355, 2587, 2922, 2923, 3389, 3441
BmiI GGNNCC 6 cut(s) 316, 728, 1045, 1496, 2356, 2924
BmrFI CCNGG 9 cut(s) 139, 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
BmrI ACTGGG 3 cut(s) 519, 791, 3988
BmsI GCATC 8 cut(s) 1099, 1119, 1999, 2047, 2444, 2572, 3217, 3718
BmtI GCTAGC 2 cut(s) 2483, 3342
BmuI ACTGGG 3 cut(s) 519, 791, 3988
BpiI GAAGAC 2 cut(s) 3463, 3753
BplI GAGNNNNNCTC 2 cut(s) 2584, 2616
BpmI CTGGAG 3 cut(s) 2707, 3197, 3698
Bpu10I CCTNAGC 3 cut(s) 1866, 2519, 3953
Bpu1102I GCTNAGC 1 cut(s) 1133
BpuEI CTTGAG 5 cut(s) 1249, 2299, 2971, 3063, 3264
BpuMI CCSGG 1 cut(s) 139
BsaAI YACGTR 2 cut(s) 601, 985
BsaBI GATNNNNATC 1 cut(s) 2043
BsaHI GRCGYC 1 cut(s) 727
BsaJI CCNNGG 6 cut(s) 318, 345, 837, 927, 1497, 3945
BsaWI WCCGGW 1 cut(s) 1376
BsaXI ACNNNNNCTCC 2 cut(s) 2485, 2515
Bsc4I CCNNNNNNNGG 6 cut(s) 821, 1529, 2005, 2783, 3815, 3946
Bse118I RCCGGY 3 cut(s) 864, 2795, 3922
Bse21I CCTNAGG 1 cut(s) 1114
Bse3DI GCAATG 4 cut(s) 172, 482, 2244, 3098
Bse8I GATNNNNATC 1 cut(s) 2043
BseBI CCWGG 8 cut(s) 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
BseDI CCNNGG 6 cut(s) 318, 345, 837, 927, 1497, 3945
BseGI GGATG 9 cut(s) 610, 691, 1970, 1993, 2014, 2204, 2697, 3232, 3733
BseJI GATNNNNATC 1 cut(s) 2043
BseLI CCNNNNNNNGG 6 cut(s) 821, 1529, 2005, 2783, 3815, 3946
BseMI GCAATG 4 cut(s) 172, 482, 2244, 3098
BseRI GAGGAG 2 cut(s) 267, 649
BseSI GKGCMC 1 cut(s) 2926
BseX3I CGGCCG 1 cut(s) 3920
BseXI GCAGC 9 cut(s) 82, 394, 2071, 2099, 2221, 2402, 2821, 3535, 3614
BseYI CCCAGC 2 cut(s) 83, 788
Bsh1236I CGCG 1 cut(s) 157
Bsh1285I CGRYCG 2 cut(s) 3923, 3996
BshNI GGYRCC 2 cut(s) 314, 726
BsiEI CGRYCG 2 cut(s) 3923, 3996
BsiHKAI GWGCWC 7 cut(s) 2284, 2890, 3067, 3183, 3213, 3632, 3987
BsiHKCI CYCGRG 1 cut(s) 2889
BsiSI CCGG 5 cut(s) 139, 865, 1377, 2796, 3923
BslFI GGGAC 4 cut(s) 805, 2368, 3503, 3559
BslI CCNNNNNNNGG 6 cut(s) 821, 1529, 2005, 2783, 3815, 3946
BsmBI CGTCTC 3 cut(s) 2317, 2758, 3705
BsmFI GGGAC 4 cut(s) 805, 2368, 3503, 3559
BsmI GAATGC 6 cut(s) 908, 1826, 1925, 2870, 3648, 3836
BsoBI CYCGRG 1 cut(s) 2889
Bsp120I GGGCCC 1 cut(s) 2922
Bsp1286I GDGCHC 8 cut(s) 2284, 2890, 2926, 3067, 3183, 3213, 3632, 3987
Bsp1407I TGTACA 1 cut(s) 3612
Bsp1720I GCTNAGC 1 cut(s) 1133
BspFNI CGCG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 991
BspLI GGNNCC 6 cut(s) 316, 728, 1045, 1496, 2356, 2924
BspMAI CTGCAG 1 cut(s) 2089
BspOI GCTAGC 2 cut(s) 2483, 3342
BspPI GGATC 6 cut(s) 330, 1009, 1404, 1710, 2940, 3925
BspQI GCTCTTC 1 cut(s) 1114
BspT107I GGYRCC 2 cut(s) 314, 726
BspTI CTTAAG 2 cut(s) 415, 883
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 4 cut(s) 172, 482, 2244, 3098
BsrFI RCCGGY 3 cut(s) 864, 2795, 3922
BsrGI TGTACA 1 cut(s) 3612
BssAI RCCGGY 3 cut(s) 864, 2795, 3922
BssECI CCNNGG 6 cut(s) 318, 345, 837, 927, 1497, 3945
BssNI GRCGYC 1 cut(s) 727
BssSI CACGAG 3 cut(s) 2601, 2837, 3882
BssT1I CCWWGG 2 cut(s) 837, 3945
Bst2BI CACGAG 3 cut(s) 2601, 2837, 3882
Bst2UI CCWGG 8 cut(s) 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
Bst4CI ACNGT 7 cut(s) 346, 432, 1051, 1955, 2139, 2362, 3448
Bst6I CTCTTC 2 cut(s) 273, 1114
BstACI GRCGYC 1 cut(s) 727
BstAFI CTTAAG 2 cut(s) 415, 883
BstAUI TGTACA 1 cut(s) 3612
BstBAI YACGTR 2 cut(s) 601, 985
BstC8I GCNNGC 9 cut(s) 78, 423, 640, 2292, 2302, 2481, 3340, 3377, 3924
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 9 cut(s) 610, 691, 1970, 1993, 2014, 2204, 2697, 3232, 3733
BstFNI CGCG 1 cut(s) 157
BstH2I RGCGCY 2 cut(s) 730, 1919
BstHHI GCGC 3 cut(s) 427, 729, 1918
BstMCI CGRYCG 2 cut(s) 3923, 3996
BstMWI GCNNNNNNNGC 8 cut(s) 338, 794, 1052, 1570, 1922, 2084, 2470, 2842
BstNI CCWGG 8 cut(s) 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
BstNSI RCATGY 4 cut(s) 2566, 3098, 3511, 3540
BstSCI CCNGG 9 cut(s) 137, 317, 926, 1205, 1497, 1522, 2607, 2684, 3174
BstSFI CTRYAG 2 cut(s) 2085, 3871
BstSLI GKGCMC 1 cut(s) 2926
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 9 cut(s) 82, 394, 2071, 2099, 2221, 2402, 2821, 3535, 3614
BstV2I GAAGAC 2 cut(s) 3463, 3753
BstX2I RGATCY 4 cut(s) 322, 1396, 1551, 3283
BstYI RGATCY 4 cut(s) 322, 1396, 1551, 3283
BstZI CGGCCG 1 cut(s) 3920
Bsu36I CCTNAGG 1 cut(s) 1114
BsuI GTATCC 1 cut(s) 759
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 2 cut(s) 657, 2075
BtrI CACGTC 2 cut(s) 2768, 3018
BtsCI GGATG 9 cut(s) 610, 691, 1970, 1993, 2014, 2204, 2697, 3232, 3733
Cac8I GCNNGC 9 cut(s) 78, 423, 640, 2292, 2302, 2481, 3340, 3377, 3924
CaiI CAGNNNCTG 2 cut(s) 277, 3641
CciI TCATGA 1 cut(s) 991
CfoI GCGC 3 cut(s) 427, 729, 1918
Cfr10I RCCGGY 3 cut(s) 864, 2795, 3922
Cfr13I GGNCC 9 cut(s) 1044, 1442, 1494, 2355, 2587, 2922, 2923, 3389, 3441
CseI GACGC 2 cut(s) 42, 146
Csp6I GTAC 6 cut(s) 502, 647, 779, 862, 2128, 3613
CspCI CAANNNNNGTGG 2 cut(s) 2248, 2283
CviQI GTAC 6 cut(s) 502, 647, 779, 862, 2128, 3613
DinI GGCGCC 1 cut(s) 728
DraIII CACNNNGTG 1 cut(s) 1598
DrdI GACNNNNNNGTC 1 cut(s) 3893
DseDI GACNNNNNNGTC 1 cut(s) 3893
EaeI YGGCCR 3 cut(s) 1627, 1776, 3920
EagI CGGCCG 1 cut(s) 3920
Eam1104I CTCTTC 2 cut(s) 273, 1114
EarI CTCTTC 2 cut(s) 273, 1114
Ecl136II GAGCTC 6 cut(s) 2282, 2888, 3181, 3211, 3630, 3985
EclXI CGGCCG 1 cut(s) 3920
Eco130I CCWWGG 2 cut(s) 837, 3945
Eco147I AGGCCT 1 cut(s) 145
Eco24I GRGCYC 7 cut(s) 2284, 2890, 2926, 3183, 3213, 3632, 3987
Eco32I GATATC 4 cut(s) 1148, 3310, 3436, 3481
Eco47I GGWCC 4 cut(s) 1442, 2355, 2587, 3389
Eco47III AGCGCT 1 cut(s) 1917
Eco52I CGGCCG 1 cut(s) 3920
Eco53kI GAGCTC 6 cut(s) 2282, 2888, 3181, 3211, 3630, 3985
Eco57I CTGAAG 3 cut(s) 254, 1052, 3396
Eco72I CACGTG 2 cut(s) 601, 985
Eco81I CCTNAGG 1 cut(s) 1114
Eco88I CYCGRG 1 cut(s) 2889
EcoICRI GAGCTC 6 cut(s) 2282, 2888, 3181, 3211, 3630, 3985
EcoO109I RGGNCCY 3 cut(s) 1442, 2922, 2923
EcoRII CCWGG 8 cut(s) 317, 926, 1205, 1497, 1522, 2607, 2684, 3174
EcoRV GATATC 4 cut(s) 1148, 3310, 3436, 3481
EcoT14I CCWWGG 2 cut(s) 837, 3945
EcoT22I ATGCAT 2 cut(s) 724, 3100
EcoT38I GRGCYC 7 cut(s) 2284, 2890, 2926, 3183, 3213, 3632, 3987
EgeI GGCGCC 1 cut(s) 728
EheI GGCGCC 1 cut(s) 728
ErhI CCWWGG 2 cut(s) 837, 3945
Esp3I CGTCTC 3 cut(s) 2317, 2758, 3705
FaqI GGGAC 4 cut(s) 805, 2368, 3503, 3559
FauI CCCGC 2 cut(s) 27, 3528
FauNDI CATATG 2 cut(s) 739, 975
FbaI TGATCA 2 cut(s) 214, 994
FokI GGATG 9 cut(s) 617, 698, 1977, 2000, 2021, 2191, 2704, 3239, 3740
FriOI GRGCYC 7 cut(s) 2284, 2890, 2926, 3183, 3213, 3632, 3987
GlaI GCGC 3 cut(s) 426, 728, 1917
GsaI CCCAGC 2 cut(s) 87, 792
GsuI CTGGAG 3 cut(s) 2707, 3197, 3698
HaeII RGCGCY 2 cut(s) 730, 1919
HapII CCGG 5 cut(s) 139, 865, 1377, 2796, 3923
HgaI GACGC 2 cut(s) 42, 146
HhaI GCGC 3 cut(s) 427, 729, 1918
Hin1I GRCGYC 1 cut(s) 727
Hin6I GCGC 3 cut(s) 425, 727, 1916
HinP1I GCGC 3 cut(s) 425, 727, 1916
HincII GTYRAC 4 cut(s) 27, 2619, 2668, 2908
HindII GTYRAC 4 cut(s) 27, 2619, 2668, 2908
HindIII AAGCTT 1 cut(s) 3130
HpaI GTTAAC 2 cut(s) 27, 2619
HpaII CCGG 5 cut(s) 139, 865, 1377, 2796, 3923
HphI GGTGA 9 cut(s) 1292, 1393, 1748, 1967, 2145, 2674, 2798, 2810, 3095
Hpy166II GTNNAC 8 cut(s) 27, 115, 1640, 2619, 2668, 2682, 2908, 3615
Hpy8I GTNNAC 8 cut(s) 27, 115, 1640, 2619, 2668, 2682, 2908, 3615
Hpy99I CGWCG 1 cut(s) 3847
HpyAV CCTTC 9 cut(s) 15, 825, 848, 1415, 1952, 2009, 2463, 2810, 3965
HpyCH4III ACNGT 7 cut(s) 346, 432, 1051, 1955, 2139, 2362, 3448
HpyCH4IV ACGT 7 cut(s) 600, 984, 2247, 2767, 3017, 3714, 3790
HpyF10VI GCNNNNNNNGC 8 cut(s) 338, 794, 1052, 1570, 1922, 2084, 2470, 2842
HpySE526I ACGT 7 cut(s) 600, 984, 2247, 2767, 3017, 3714, 3790
Hsp92I GRCGYC 1 cut(s) 727
HspAI GCGC 3 cut(s) 425, 727, 1916
KasI GGCGCC 1 cut(s) 726
KroI GCCGGC 1 cut(s) 3922
KroNI GCCGGC 1 cut(s) 3924
Ksp22I TGATCA 2 cut(s) 214, 994
KspAI GTTAAC 2 cut(s) 27, 2619
LguI GCTCTTC 1 cut(s) 1114
Lsp1109I GCAGC 9 cut(s) 82, 394, 2071, 2099, 2221, 2402, 2821, 3535, 3614
LweI GCATC 8 cut(s) 1099, 1119, 1999, 2047, 2444, 2572, 3217, 3718
MaeII ACGT 7 cut(s) 600, 984, 2247, 2767, 3017, 3714, 3790
MbiI CCGCTC 1 cut(s) 34
MfeI CAATTG 1 cut(s) 3739
MflI RGATCY 4 cut(s) 322, 1396, 1551, 3283
MhlI GDGCHC 8 cut(s) 2284, 2890, 2926, 3067, 3183, 3213, 3632, 3987
MlsI TGGCCA 2 cut(s) 1629, 1778
MluNI TGGCCA 2 cut(s) 1629, 1778
Mly113I GGCGCC 1 cut(s) 727
MmeI TCCRAC 2 cut(s) 590, 1397
Mox20I TGGCCA 2 cut(s) 1629, 1778
Mph1103I ATGCAT 2 cut(s) 724, 3100
MroNI GCCGGC 1 cut(s) 3922
MroXI GAANNNNTTC 3 cut(s) 808, 1192, 3409
MscI TGGCCA 2 cut(s) 1629, 1778
MslI CAYNNNNRTG 3 cut(s) 603, 1155, 2738
Msp20I TGGCCA 2 cut(s) 1629, 1778
MspA1I CMGCKG 1 cut(s) 2624
MspCI CTTAAG 2 cut(s) 415, 883
MspI CCGG 5 cut(s) 139, 865, 1377, 2796, 3923
MspR9I CCNGG 9 cut(s) 139, 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
MunI CAATTG 1 cut(s) 3739
Mva1269I GAATGC 6 cut(s) 908, 1826, 1925, 2870, 3648, 3836
MvaI CCWGG 8 cut(s) 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 8 cut(s) 338, 794, 1052, 1570, 1922, 2084, 2470, 2842
NaeI GCCGGC 1 cut(s) 3924
NarI GGCGCC 1 cut(s) 727
NciI CCSGG 1 cut(s) 139
NdeI CATATG 2 cut(s) 739, 975
NgoMIV GCCGGC 1 cut(s) 3922
NheI GCTAGC 2 cut(s) 2479, 3338
NlaIV GGNNCC 6 cut(s) 316, 728, 1045, 1496, 2356, 2924
NmuCI GTSAC 8 cut(s) 980, 1598, 1834, 2603, 2989, 3013, 3382, 3895
NsiI ATGCAT 2 cut(s) 724, 3100
NspI RCATGY 4 cut(s) 2566, 3098, 3511, 3540
OliI CACNNNNGTG 1 cut(s) 1155
PaeR7I CTCGAG 1 cut(s) 2889
PagI TCATGA 1 cut(s) 991
PceI AGGCCT 1 cut(s) 145
PciI ACATGT 2 cut(s) 2562, 3536
PciSI GCTCTTC 1 cut(s) 1114
PctI GAATGC 6 cut(s) 908, 1826, 1925, 2870, 3648, 3836
PdiI GCCGGC 1 cut(s) 3924
PdmI GAANNNNTTC 3 cut(s) 808, 1192, 3409
PflMI CCANNNNNTGG 2 cut(s) 1529, 3946
PfoI TCCNGGA 2 cut(s) 137, 3174
Ple19I CGATCG 1 cut(s) 3996
PluTI GGCGCC 1 cut(s) 730
PmaCI CACGTG 2 cut(s) 601, 985
PmlI CACGTG 2 cut(s) 601, 985
Ppu21I YACGTR 2 cut(s) 601, 985
PpuMI RGGWCCY 1 cut(s) 1442
PscI ACATGT 2 cut(s) 2562, 3536
Psp124BI GAGCTC 6 cut(s) 2284, 2890, 3183, 3213, 3632, 3987
Psp5II RGGWCCY 1 cut(s) 1442
Psp6I CCWGG 8 cut(s) 317, 926, 1205, 1497, 1522, 2607, 2684, 3174
PspCI CACGTG 2 cut(s) 601, 985
PspFI CCCAGC 2 cut(s) 83, 788
PspGI CCWGG 8 cut(s) 317, 926, 1205, 1497, 1522, 2607, 2684, 3174
PspN4I GGNNCC 6 cut(s) 316, 728, 1045, 1496, 2356, 2924
PspOMI GGGCCC 1 cut(s) 2922
PspPI GGNCC 9 cut(s) 1044, 1442, 1494, 2355, 2587, 2922, 2923, 3389, 3441
PspPPI RGGWCCY 1 cut(s) 1442
PstI CTGCAG 1 cut(s) 2089
PstNI CAGNNNCTG 2 cut(s) 277, 3641
PsuI RGATCY 4 cut(s) 322, 1396, 1551, 3283
PvuI CGATCG 1 cut(s) 3996
RsaI GTAC 6 cut(s) 503, 648, 780, 863, 2129, 3614
RsaNI GTAC 6 cut(s) 502, 647, 779, 862, 2128, 3613
RseI CAYNNNNRTG 3 cut(s) 603, 1155, 2738
SacI GAGCTC 6 cut(s) 2284, 2890, 3183, 3213, 3632, 3987
SapI GCTCTTC 1 cut(s) 1114
Sau96I GGNCC 9 cut(s) 1044, 1442, 1494, 2355, 2587, 2922, 2923, 3389, 3441
ScrFI CCNGG 9 cut(s) 139, 319, 928, 1207, 1499, 1524, 2609, 2686, 3176
SduI GDGCHC 8 cut(s) 2284, 2890, 2926, 3067, 3183, 3213, 3632, 3987
SfaNI GCATC 8 cut(s) 1099, 1119, 1999, 2047, 2444, 2572, 3217, 3718
SfcI CTRYAG 2 cut(s) 2085, 3871
SfoI GGCGCC 1 cut(s) 728
Sfr274I CTCGAG 1 cut(s) 2889
SinI GGWCC 4 cut(s) 1442, 2355, 2587, 3389
SlaI CTCGAG 1 cut(s) 2889
SmiMI CAYNNNNRTG 3 cut(s) 603, 1155, 2738
SmlI CTYRAG 8 cut(s) 415, 883, 1264, 2314, 2889, 2950, 3078, 3279
SmoI CTYRAG 8 cut(s) 415, 883, 1264, 2314, 2889, 2950, 3078, 3279
SpeI ACTAGT 2 cut(s) 334, 1154
SseBI AGGCCT 1 cut(s) 145
SspDI GGCGCC 1 cut(s) 726
SspI AATATT 2 cut(s) 457, 1694
SstI GAGCTC 6 cut(s) 2284, 2890, 3183, 3213, 3632, 3987
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 9 cut(s) 137, 317, 926, 1205, 1497, 1522, 2607, 2684, 3174
StyI CCWWGG 2 cut(s) 837, 3945
TaaI ACNGT 7 cut(s) 346, 432, 1051, 1955, 2139, 2362, 3448
TaiI ACGT 7 cut(s) 603, 987, 2250, 2770, 3020, 3717, 3793
TaqI TCGA 8 cut(s) 1071, 1109, 1408, 1873, 2890, 3213, 3992, 3996
TaqII GACCGA 2 cut(s) 1254, 2349
TatI WGTACW 3 cut(s) 501, 778, 3612
TauI GCSGC 4 cut(s) 83, 1213, 1567, 1865
TseFI GTSAC 8 cut(s) 980, 1598, 1834, 2603, 2989, 3013, 3382, 3895
TseI GCWGC 9 cut(s) 95, 407, 2084, 2087, 2234, 2390, 2809, 3523, 3602
Tsp45I GTSAC 8 cut(s) 980, 1598, 1834, 2603, 2989, 3013, 3382, 3895
TspGWI ACGGA 4 cut(s) 76, 392, 2021, 3977
Van91I CCANNNNNTGG 2 cut(s) 1529, 3946
Vha464I CTTAAG 2 cut(s) 415, 883
VpaK11BI GGWCC 4 cut(s) 1442, 2355, 2587, 3389
XapI RAATTY 4 cut(s) 711, 1802, 2915, 3201
XbaI TCTAGA 2 cut(s) 1687, 1813
XceI RCATGY 4 cut(s) 2566, 3098, 3511, 3540
XcmI CCANNNNNNNNNTGG 2 cut(s) 325, 3472
XhoI CTCGAG 1 cut(s) 2889
XmnI GAANNNNTTC 3 cut(s) 808, 1192, 3409
Zsp2I ATGCAT 2 cut(s) 724, 3100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.