Rroxscaffold_6G00397300

ABC transporter D family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006235
Physical Location & Seq
Reverse (-)
18960160 .. 18977157
16998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397300.1

Sequence Viewer

Length: 3819 bp
ATGGTTTGTGGTTCGCGTACGCCAATTCTGCATGTCTGTGTATGCTATACACTACTGAAAACCTTGCTTCTTGCTACTGGTATTGTAGTTGCTGGTGGGGCAGCTGCATATGTTCAATCGAGATTAACCCACAAAAAGCACAATTCTTTTGGTCAATATAATGGGCTGAATGAAAATAAAGTGACAGAGAATGTCGTGGCGAATGATCATAAGAAAAAGAAACTTCCACAAAAGAGAGGGGGATTGAAGTCTCTTCAAGTCCTTGCTGCAATTCTTTTGTCTGAAATGGGCCAAATGGGTGCGAGGGACCTCTTATCTTTGGTCGGAATAGTGAATATTGCATACTACAAAATATCACACGTCGATGGTCGGATAACTAATCCGGAACAGCGAATTGCAAGTGATGTACCAAGGTTCTGTTCAGAGTTGAGTGAAATCGTACAAGATGATTTAACAGCTGTTACTGATGGTCTCCTTTACTCTTGGCGGCTGTGTTCCTACACCAGTCCTAAATATATATTCTGGATATTGGCATATGTATTGGGAGCAGGGGGGATGATAAGAAAGTTCTCTCCTCCTTTTGGGAAATTAATGTCCAAAGAACAACAATTGGAAGGGGAGTACCGACAGCTTCATTCACGATTAAGAACCCATGCAGAAAGTGTGGCATTCTATGGTGGAGAAAGTAGAGAGGAATCTCATATTCAGAAGAAGTTTAACACACTGGTTGGGCATCTGCGTGTTGTCCTTCATGATCATTGGTGGTTTGGCATGATTCAAGACTTCCTGTTGAAGTATCTTGGTGCCACAGTTGCTGTTATATTGATTATTGAGCCCTTCTTTTCGGGCAGTTTGAGGCCTGACACTTCAACTTTAGGCCGCGCAGAGATGTTAAGCAACTTAAGATATCATACCAGTGTCATAATTTCACTTTTCCAGTCTATGGGAACTCTCGCTTCAAGTTCGAGAAAACTCAATCGTCTCAGTGGTTACGCTGATCGCATTCATGAATTAATGGTCATATCAAGAGAGCTAAGTGCTGTTGATGACAAATCTTCTGGGAGTAAAAATTGCTCTAGTGAGGCTGATTACATTGAATTTGCTGGGGTCAAGGTTGTTACCCCCACGGGTAATGTTTTGGTGGATAAATTGTCACTTAGGGTTGAACCAGGATCTAATCTTTTGATTACAGGTCCAAATGGCAGTGGAAAGAGCTCGCTTTTCCGAGTTTTAGGAGGCCTGTGGCCCTTGGTATCTGGCCATATTGTGAAACCTGGTGTTGGCACTGATCTCAACAAAGAAATTTTCTATGTTCCACAAAGACCATACACAGCTGTCGGAACACTTCGAGACCAGTTGATTTATCCTCTTACTGCAGATCAAGAGGTCAAACCACTCACACGTGGAGAAATGGCGGAGCTATTGAGAAATGTTGATCTTCAGTATTTATTAGACCGTTATCCACCTGAGGAAGAGATAAATTGGGGTGATGAATTATCTCTTGGTGAGCAACAAAGATTGGGGATGGCTAGACTTTTCTATCACAAGCCCAAATTTGCAATTCTTGATGAGTGCACAAGTGCTGTCACAACTGACATGGAGGAACGATTCTGTGCTAAAGTTCGAGCAATGGGAACATCATGCATTACTATCTCGCATCGTCCAGCACTAGTTGCATTTCATGATGTAGTGCTGTCCTTGGATGGTGAAGGCGGTTGGAGTGTTCATGAGAAAAGGGAGGATTCTCTAGTACGTAACGAAGGTGGGAGAAGTATGTTGAAGCCTTCTGAAACGAACCGGCAGAGTGATGCAATGACAGTTCAGCGAGCATTCGCCTCGACCAAAAAGGATTCCGCAATCTCAAACTCAAAGTCCCAGTCATACATTGCTGAGGTGATAGCAGTTTCTCCCTCTGCCGAGCATAATGTTACAGTTCCGTCTTTCCCACATCTCCAAAGTGCTCCCGCAGCACTGCCATTGAGAGCATCTGCTCTGGTCAAAGTACTGATTCCCACTGTTATTGATAAACAAGGAGCACAACTGCTTGCAGTTGCTTTTCTCGTAATATCGAGGACATGGATTTCTGATCGTATTGCTTCATTAAATGGAACCACTGTGAAGTTTGTTTTGGAACAAGATAAGGCATCATTTATTCATCTAATTGGTGTTAGCGTACTTCAGAGTGCGGCATCTTCTTTCATTGCACCTTCTATAAGGCACTTAAAAGCTAGGCTGGCTCTTGGGTGGAGGACTCGTTTGACTCAACATTTACTCAAAAACTACTTGAGAAATAATGCATTCTATAAGGTTCACCTGGAGAATGAAGCTTTTGACTGGTCAGAGAGGAGTAACTATATTGTACGCGTACATGTTACTGGGTCTTGGTTTGCTCAGGGTTGCTACCCCGAATTTGGTGATTTGACAAGTCGTCAACAACAACTTGAAGGAACCTTTAGGTTTATGCATGAAAGACTGCGTGCTCATGCTGAATCTGTTGCTTTCTTTGGGGGTGGTTATAGAGAGAAAGCTATGGTTGAATCAAAGTTCAAGGAGCTACTCCATCACTCCTCGTCTCTTTTGAAGAAGCAATGGTTATTCGGTATTCTAGATGATTTTATCACGAAACAACTCCCACATAATGTGACTTGGGGATTGAGCTTGCTATATGCCATTGAACACAAGGGAGACCGAGCATTAATATCTACTCAAGGTGAGCTGGCACATGCATTGCGTTTCTTGGCATCTGTGGTATCTCAAAGCTTTTTAGCTTTCGGTGACATTCTAGAATTGCATAGAAAATTCCTGGAACTCTCTGGCAGCATCAACAGGGTTTTTGAACTTGAAGAGCTTTTAGACACTGCACAATCTGTGGTTGGCTATTCTGGGGATTGTGAAGCTGGTAACTCATCATCATCATCTGAGAGGGATGGTTTTCAATTTGAAGATGCCATTAACTTCTCTGAAGTTGATATCATAACCCCGTCACAGAAATTGTTGGCTAGGAAATTAACATGTGATATAGTACCTGGGAAAAGCTTGCTTGTTACTGGTCCAAACGGGAGTGGAAAAAGTTCTGTGTTTAGAGTTCTTAGAGGTCTTTGGCCCATCATGAGTGGAAGAATCACTAGACCATCTCAAGATGTTAACGGAGTTGGTAGAGATGTTGGATCTGGTTGCGGAGTTTTTTATGTTCCTCAGCGACCTTACACATGCTTGGGAACACTCAGGGATCAAATTATATATCCTCTCTCTTTTGATGAGGCGGAGATAAGGGCATTGAAGTTGTATCAAGAAGGTGGAAAATTTACTGACAGCACCAACATTCTGGACATGCGTTTGAGAACTATTCTGGAGAACGTCCGATTAAGTTATCTTTTGGAAAGGGAGGAAGGTGGTTGGGATGCTAATCTCAATTGGGAGGACACTCTCTCTCTCGGAGAACAGCAGAGATTAGGCATGGCACGCTTATTTTTTCACAAACCAAAATTCGCCATCCTTGATGAATGCACCAATGCTACAAGTGTTGATGTTGAAGAACAACTCTATCGGCTTGCAAAAGACATGGGTATCACAGTTGTTACCTCCTCACAACGTCCTGCTCTAATACCATTCCATTCCCTGGAATTACGGCTTATTGATGGTGAGGGTCCACTGGTTACCTTGTCCGGCGTTGAGAAGAGGGAGACGGTTCTTTGTGGAGAAGAGATCTTGCGCACATTTGGTGCATTTGATGATTTGATTTTGCCAAGGCTGGATCAGGAAAGGGACTCATTTTTAAAGTGGAGGATCTTGATATTGAAAGGAAGAGATACTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006629 GO:0008150 GO:0008152 GO:0009056 GO:0016042 GO:0044238 GO:0071704 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko02010 ko04146 map02010 map04146
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

1272

Amino Acids

141.85

Weight (kDa)

8.15

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 112 - 281 4.3e-60 ABC transporter transmembrane region 2
ABC_tran PF00005 382 - 527 1.5e-19 ABC transporter
ABC_membrane_2 PF06472 665 - 772 3.5e-25 ABC transporter transmembrane region 2
ABC_membrane_2 PF06472 796 - 889 2.7e-25 ABC transporter transmembrane region 2
ABC_tran PF00005 1002 - 1171 1.3e-15 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3713
AccB1I GGYRCC 1 cut(s) 803
AccB7I CCANNNNNTGG 2 cut(s) 943, 3619
AccII CGCG 3 cut(s) 16, 882, 2364
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 9 cut(s) 487, 880, 1415, 1713, 1854, 1965, 2186, 3177, 3263
AclWI GGATC 5 cut(s) 1180, 3175, 3237, 3762, 3794
AcoI YGGCCR 1 cut(s) 1258
AcsI RAATTY 7 cut(s) 1097, 1302, 1553, 2408, 2798, 3302, 3485
AcuI CTGAAG 3 cut(s) 1424, 2162, 2982
AcvI CACGTG 1 cut(s) 1403
AfiI CCNNNNNNNGG 5 cut(s) 581, 943, 1280, 2411, 3619
AflII CTTAAG 1 cut(s) 901
AflIII ACRYGT 5 cut(s) 358, 1400, 2362, 2368, 3011
AhdI GACNNNNNGTC 1 cut(s) 2427
AhlI ACTAGT 1 cut(s) 1669
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 6 cut(s) 1168, 1273, 2313, 2802, 3025, 3618
AjuI GAANNNNNNNTTGG 6 cut(s) 403, 435, 1485, 1517, 2111, 2143
Alw21I GWGCWC 5 cut(s) 1217, 1577, 1963, 2038, 2482
Alw26I GTCTC 7 cut(s) 255, 476, 986, 1344, 2577, 2679, 3677
Alw44I GTGCAC 1 cut(s) 1573
AlwI GGATC 5 cut(s) 1180, 3175, 3237, 3762, 3794
AlwNI CAGNNNCTG 1 cut(s) 815
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 7 cut(s) 289, 857, 877, 1237, 1244, 1258, 3101
ApaLI GTGCAC 1 cut(s) 1573
ApeKI GCWGC 5 cut(s) 101, 104, 266, 1967, 2817
ApoI RAATTY 7 cut(s) 1097, 1302, 1553, 2408, 2798, 3302, 3485
ArsI GACNNNNNNTTYG 4 cut(s) 1856, 1862, 1888, 1894
AseI ATTAAT 3 cut(s) 590, 1013, 2696
Asp700I GAANNNNTTC 1 cut(s) 3070
AspLEI GCGC 2 cut(s) 884, 3714
AspS9I GGNCC 7 cut(s) 289, 307, 1193, 1245, 3050, 3102, 3647
AsuHPI GGTGA 9 cut(s) 1499, 1517, 1718, 1906, 2303, 2426, 2723, 2786, 3653
AvaII GGWCC 4 cut(s) 307, 1193, 3050, 3647
AxyI CCTNAGG 1 cut(s) 1467
BaeGI GKGCMC 1 cut(s) 1577
BaeI ACNNNNGTAYC 2 cut(s) 3550, 3583
BalI TGGCCA 1 cut(s) 1260
BanI GGYRCC 1 cut(s) 803
BanII GRGCYC 2 cut(s) 837, 1217
BbrPI CACGTG 1 cut(s) 1403
Bbv12I GWGCWC 5 cut(s) 1217, 1577, 1963, 2038, 2482
BbvCI CCTCAGC 2 cut(s) 1890, 3195
BbvI GCAGC 5 cut(s) 91, 113, 253, 1979, 2829
BceAI ACGGC 1 cut(s) 3644
BcgI CGANNNNNNTGC 2 cut(s) 1818, 1852
BciT130I CCWGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
BclI TGATCA 2 cut(s) 205, 754
BcoDI GTCTC 7 cut(s) 255, 476, 986, 1344, 2577, 2679, 3677
BcuI ACTAGT 1 cut(s) 1669
BfaI CTAG 9 cut(s) 1077, 1530, 1670, 1748, 2229, 2606, 2783, 3000, 3126
BfmI CTRYAG 1 cut(s) 1374
BfrI CTTAAG 1 cut(s) 901
BglII AGATCT 1 cut(s) 3705
BisI GCNGC 8 cut(s) 102, 105, 267, 488, 880, 1968, 2187, 2818
BlsI GCNGC 8 cut(s) 103, 106, 268, 489, 881, 1969, 2188, 2819
BmcAI AGTACT 1 cut(s) 2004
Bme1390I CCNGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
Bme18I GGWCC 4 cut(s) 307, 1193, 3050, 3647
BmeRI GACNNNNNGTC 1 cut(s) 2427
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 7 cut(s) 289, 307, 1193, 1245, 3050, 3102, 3647
BmiI GGNNCC 5 cut(s) 308, 805, 2110, 2449, 3648
BmrFI CCNGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
BmrI ACTGGG 2 cut(s) 1870, 2385
BmuI ACTGGG 2 cut(s) 1870, 2385
BpmI CTGGAG 2 cut(s) 2336, 3371
Bpu10I CCTNAGC 3 cut(s) 1890, 2391, 3195
BpuEI CTTGAG 3 cut(s) 2305, 2691, 3120
BsaAI YACGTR 2 cut(s) 1403, 1754
BsaBI GATNNNNATC 1 cut(s) 3405
BsaI GGTCTC 3 cut(s) 476, 1344, 2679
BsaJI CCNNGG 7 cut(s) 410, 1125, 1248, 1698, 3026, 3618, 3746
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 2239, 2269
Bsc4I CCNNNNNNNGG 5 cut(s) 581, 943, 1280, 2411, 3619
Bse118I RCCGGY 1 cut(s) 1797
Bse21I CCTNAGG 1 cut(s) 1467
Bse3DI GCAATG 6 cut(s) 1635, 1818, 1884, 2199, 2594, 2726
Bse8I GATNNNNATC 1 cut(s) 3405
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
BseDI CCNNGG 7 cut(s) 410, 1125, 1248, 1698, 3026, 3618, 3746
BseGI GGATG 6 cut(s) 561, 1530, 1708, 2932, 3406, 3492
BseJI GATNNNNATC 1 cut(s) 3405
BseLI CCNNNNNNNGG 5 cut(s) 581, 943, 1280, 2411, 3619
BseMI GCAATG 6 cut(s) 1635, 1818, 1884, 2199, 2594, 2726
BseMII CTCAG 7 cut(s) 997, 1458, 1881, 2405, 2910, 3209, 3238
BseRI GAGGAG 4 cut(s) 564, 2359, 2557, 3574
BseSI GKGCMC 1 cut(s) 1577
BseXI GCAGC 5 cut(s) 91, 113, 253, 1979, 2829
BseYI CCCAGC 1 cut(s) 1103
BsgI GTGCAG 1 cut(s) 2844
Bsh1236I CGCG 3 cut(s) 16, 882, 2364
BshFI GGCC 7 cut(s) 291, 859, 879, 1239, 1246, 1260, 3103
BshNI GGYRCC 1 cut(s) 803
BsiHKAI GWGCWC 5 cut(s) 1217, 1577, 1963, 2038, 2482
BsiSI CCGG 3 cut(s) 383, 1798, 3666
BsiWI CGTACG 1 cut(s) 17
BslFI GGGAC 3 cut(s) 320, 1858, 3779
BslI CCNNNNNNNGG 5 cut(s) 581, 943, 1280, 2411, 3619
BsmAI GTCTC 7 cut(s) 255, 476, 986, 1344, 2577, 2679, 3677
BsmBI CGTCTC 3 cut(s) 986, 2577, 3677
BsmFI GGGAC 3 cut(s) 320, 1858, 3779
BsmI GAATGC 5 cut(s) 668, 1002, 1829, 2297, 3509
BsnI GGCC 7 cut(s) 291, 859, 879, 1239, 1246, 1260, 3103
Bso31I GGTCTC 3 cut(s) 476, 1344, 2679
Bsp1286I GDGCHC 6 cut(s) 837, 1217, 1577, 1963, 2038, 2482
Bsp13I TCCGGA 1 cut(s) 382
BspACI CCGC 9 cut(s) 487, 880, 1415, 1713, 1854, 1965, 2186, 3177, 3263
BspANI GGCC 7 cut(s) 291, 859, 879, 1239, 1246, 1260, 3103
BspCNI CTCAG 7 cut(s) 996, 1459, 1882, 2404, 2911, 3208, 3237
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 3 cut(s) 16, 882, 2364
BspHI TCATGA 5 cut(s) 751, 1006, 1681, 1726, 3108
BspLI GGNNCC 5 cut(s) 308, 805, 2110, 2449, 3648
BspMAI CTGCAG 1 cut(s) 1378
BspPI GGATC 5 cut(s) 1180, 3175, 3237, 3762, 3794
BspQI GCTCTTC 1 cut(s) 2838
BspT107I GGYRCC 1 cut(s) 803
BspTI CTTAAG 1 cut(s) 901
BspTNI GGTCTC 3 cut(s) 476, 1344, 2679
BsrDI GCAATG 6 cut(s) 1635, 1818, 1884, 2199, 2594, 2726
BsrFI RCCGGY 1 cut(s) 1797
BssAI RCCGGY 1 cut(s) 1797
BssECI CCNNGG 7 cut(s) 410, 1125, 1248, 1698, 3026, 3618, 3746
BssT1I CCWWGG 4 cut(s) 410, 1248, 1698, 3746
Bst2UI CCWGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
Bst4CI ACNGT 8 cut(s) 811, 1457, 1819, 1933, 2017, 2116, 3574, 3688
Bst6I CTCTTC 6 cut(s) 258, 1467, 2838, 3671, 3696, 3799
BstAFI CTTAAG 1 cut(s) 901
BstAPI GCANNNNNTGC 1 cut(s) 1673
BstBAI YACGTR 2 cut(s) 1403, 1754
BstDSI CCRYGG 1 cut(s) 1125
BstEII GGTNACC 1 cut(s) 3655
BstF5I GGATG 6 cut(s) 561, 1530, 1708, 2932, 3406, 3492
BstFNI CGCG 3 cut(s) 16, 882, 2364
BstHHI GCGC 2 cut(s) 884, 3714
BstMAI GTCTC 7 cut(s) 255, 476, 986, 1344, 2577, 2679, 3677
BstMWI GCNNNNNNNGC 7 cut(s) 28, 98, 812, 1673, 1967, 2234, 3462
BstNI CCWGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
BstNSI RCATGY 6 cut(s) 35, 2372, 2726, 3015, 3213, 3334
BstPI GGTNACC 1 cut(s) 3655
BstSCI CCNGG 6 cut(s) 1168, 1273, 2313, 2802, 3025, 3618
BstSFI CTRYAG 1 cut(s) 1374
BstSLI GKGCMC 1 cut(s) 1577
BstSNI TACGTA 1 cut(s) 1754
BstUI CGCG 3 cut(s) 16, 882, 2364
BstV1I GCAGC 5 cut(s) 91, 113, 253, 1979, 2829
BstX2I RGATCY 4 cut(s) 1172, 3167, 3705, 3786
BstXI CCANNNNNNTGG 1 cut(s) 3325
BstYI RGATCY 4 cut(s) 1172, 3167, 3705, 3786
Bsu36I CCTNAGG 1 cut(s) 1467
BsuRI GGCC 7 cut(s) 291, 859, 879, 1239, 1246, 1260, 3103
BtgI CCRYGG 1 cut(s) 1125
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 6 cut(s) 561, 1530, 1708, 2932, 3406, 3492
BtsI GCAGTG 3 cut(s) 1210, 1970, 2856
CaiI CAGNNNCTG 1 cut(s) 815
CciI TCATGA 5 cut(s) 751, 1006, 1681, 1726, 3108
CfoI GCGC 2 cut(s) 884, 3714
Cfr10I RCCGGY 1 cut(s) 1797
Cfr13I GGNCC 7 cut(s) 289, 307, 1193, 1245, 3050, 3102, 3647
CsiI ACCWGGT 1 cut(s) 1273
CspCI CAANNNNNGTGG 2 cut(s) 2002, 2037
DraI TTTAAA 1 cut(s) 3777
DriI GACNNNNNGTC 1 cut(s) 2427
EaeI YGGCCR 1 cut(s) 1258
Eam1104I CTCTTC 6 cut(s) 258, 1467, 2838, 3671, 3696, 3799
Eam1105I GACNNNNNGTC 1 cut(s) 2427
EarI CTCTTC 6 cut(s) 258, 1467, 2838, 3671, 3696, 3799
EciI GGCGGA 2 cut(s) 1430, 3278
Ecl136II GAGCTC 1 cut(s) 1215
Eco105I TACGTA 1 cut(s) 1754
Eco130I CCWWGG 4 cut(s) 410, 1248, 1698, 3746
Eco147I AGGCCT 2 cut(s) 859, 1239
Eco24I GRGCYC 2 cut(s) 837, 1217
Eco31I GGTCTC 3 cut(s) 476, 1344, 2679
Eco32I GATATC 2 cut(s) 908, 2971
Eco47I GGWCC 4 cut(s) 307, 1193, 3050, 3647
Eco53kI GAGCTC 1 cut(s) 1215
Eco57I CTGAAG 3 cut(s) 1424, 2162, 2982
Eco72I CACGTG 1 cut(s) 1403
Eco81I CCTNAGG 1 cut(s) 1467
Eco91I GGTNACC 1 cut(s) 3655
EcoICRI GAGCTC 1 cut(s) 1215
EcoO109I RGGNCCY 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 3655
EcoRII CCWGG 6 cut(s) 1168, 1273, 2313, 2802, 3025, 3618
EcoRV GATATC 2 cut(s) 908, 2971
EcoT14I CCWWGG 4 cut(s) 410, 1248, 1698, 3746
EcoT22I ATGCAT 4 cut(s) 1646, 2299, 2466, 2728
EcoT38I GRGCYC 2 cut(s) 837, 1217
ErhI CCWWGG 4 cut(s) 410, 1248, 1698, 3746
Esp3I CGTCTC 3 cut(s) 986, 2577, 3677
FaqI GGGAC 3 cut(s) 320, 1858, 3779
FauI CCCGC 1 cut(s) 1972
FauNDI CATATG 2 cut(s) 109, 535
FbaI TGATCA 2 cut(s) 205, 754
Fnu4HI GCNGC 8 cut(s) 102, 105, 267, 488, 880, 1968, 2187, 2818
FokI GGATG 6 cut(s) 568, 1537, 1715, 2939, 3413, 3479
FriOI GRGCYC 2 cut(s) 837, 1217
Fsp4HI GCNGC 8 cut(s) 102, 105, 267, 488, 880, 1968, 2187, 2818
FspBI CTAG 9 cut(s) 1077, 1530, 1670, 1748, 2229, 2606, 2783, 3000, 3126
FspI TGCGCA 1 cut(s) 3713
GlaI GCGC 2 cut(s) 883, 3713
GluI GCNGC 8 cut(s) 102, 105, 267, 488, 880, 1968, 2187, 2818
GsaI CCCAGC 1 cut(s) 1107
GsuI CTGGAG 2 cut(s) 2336, 3371
HaeIII GGCC 7 cut(s) 291, 859, 879, 1239, 1246, 1260, 3103
HapII CCGG 3 cut(s) 383, 1798, 3666
HhaI GCGC 2 cut(s) 884, 3714
Hin6I GCGC 2 cut(s) 882, 3712
HinP1I GCGC 2 cut(s) 882, 3712
HincII GTYRAC 2 cut(s) 2432, 3145
HindII GTYRAC 2 cut(s) 2432, 3145
HindIII AAGCTT 3 cut(s) 2325, 2758, 3034
HpaI GTTAAC 1 cut(s) 3145
HpaII CCGG 3 cut(s) 383, 1798, 3666
HphI GGTGA 9 cut(s) 1499, 1517, 1718, 1906, 2303, 2426, 2723, 2786, 3653
Hpy166II GTNNAC 5 cut(s) 1575, 2311, 2432, 3145, 3650
Hpy8I GTNNAC 5 cut(s) 1575, 2311, 2432, 3145, 3650
Hpy99I CGWCG 1 cut(s) 365
HpyCH4III ACNGT 8 cut(s) 811, 1457, 1819, 1933, 2017, 2116, 3574, 3688
HpyCH4IV ACGT 5 cut(s) 360, 1402, 1753, 3357, 3592
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 98, 812, 1673, 1967, 2234, 3462
HpySE526I ACGT 5 cut(s) 360, 1402, 1753, 3357, 3592
HspAI GCGC 2 cut(s) 882, 3712
Kpn2I TCCGGA 1 cut(s) 382
Ksp22I TGATCA 2 cut(s) 205, 754
KspAI GTTAAC 1 cut(s) 3145
LguI GCTCTTC 1 cut(s) 2838
LmnI GCTCC 5 cut(s) 545, 1417, 1966, 2033, 2551
Lsp1109I GCAGC 5 cut(s) 91, 113, 253, 1979, 2829
MabI ACCWGGT 1 cut(s) 1273
MaeI CTAG 9 cut(s) 1077, 1530, 1670, 1748, 2229, 2606, 2783, 3000, 3126
MaeII ACGT 5 cut(s) 360, 1402, 1753, 3357, 3592
MfeI CAATTG 2 cut(s) 608, 3412
MflI RGATCY 4 cut(s) 1172, 3167, 3705, 3786
MhlI GDGCHC 6 cut(s) 837, 1217, 1577, 1963, 2038, 2482
MlsI TGGCCA 1 cut(s) 1260
MluI ACGCGT 1 cut(s) 2362
MluNI TGGCCA 1 cut(s) 1260
MlyI GAGTC 3 cut(s) 2245, 2254, 3761
MmeI TCCRAC 5 cut(s) 304, 350, 1318, 1697, 3145
Mox20I TGGCCA 1 cut(s) 1260
Mph1103I ATGCAT 4 cut(s) 1646, 2299, 2466, 2728
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 3070
MscI TGGCCA 1 cut(s) 1260
MslI CAYNNNNRTG 4 cut(s) 36, 363, 738, 915
Msp20I TGGCCA 1 cut(s) 1260
MspA1I CMGCKG 3 cut(s) 104, 458, 1334
MspCI CTTAAG 1 cut(s) 901
MspI CCGG 3 cut(s) 383, 1798, 3666
MspR9I CCNGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
MunI CAATTG 2 cut(s) 608, 3412
Mva1269I GAATGC 5 cut(s) 668, 1002, 1829, 2297, 3509
MvaI CCWGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
MvnI CGCG 3 cut(s) 16, 882, 2364
MwoI GCNNNNNNNGC 7 cut(s) 28, 98, 812, 1673, 1967, 2234, 3462
NdeI CATATG 2 cut(s) 109, 535
NlaIV GGNNCC 5 cut(s) 308, 805, 2110, 2449, 3648
NmeAIII GCCGAG 1 cut(s) 1942
NmuCI GTSAC 6 cut(s) 181, 1152, 1585, 2641, 2774, 2982
NsbI TGCGCA 1 cut(s) 3713
NsiI ATGCAT 4 cut(s) 1646, 2299, 2466, 2728
NspI RCATGY 6 cut(s) 35, 2372, 2726, 3015, 3213, 3334
PagI TCATGA 5 cut(s) 751, 1006, 1681, 1726, 3108
PceI AGGCCT 2 cut(s) 859, 1239
PciI ACATGT 2 cut(s) 2368, 3011
PciSI GCTCTTC 1 cut(s) 2838
PctI GAATGC 5 cut(s) 668, 1002, 1829, 2297, 3509
PdmI GAANNNNTTC 1 cut(s) 3070
PfeI GAWTC 9 cut(s) 695, 775, 1608, 1742, 1850, 2008, 2489, 2537, 3120
Pfl23II CGTACG 1 cut(s) 17
PflMI CCANNNNNTGG 2 cut(s) 943, 3619
PfoI TCCNGGA 1 cut(s) 2802
PkrI GCNGC 8 cut(s) 103, 106, 268, 489, 881, 1969, 2188, 2819
PleI GAGTC 3 cut(s) 2245, 2254, 3761
PmaCI CACGTG 1 cut(s) 1403
PmlI CACGTG 1 cut(s) 1403
PpsI GAGTC 3 cut(s) 2245, 2254, 3761
Ppu21I YACGTR 2 cut(s) 1403, 1754
PpuMI RGGWCCY 1 cut(s) 307
PscI ACATGT 2 cut(s) 2368, 3011
PshBI ATTAAT 3 cut(s) 590, 1013, 2696
Psp124BI GAGCTC 1 cut(s) 1217
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 6 cut(s) 1168, 1273, 2313, 2802, 3025, 3618
PspCI CACGTG 1 cut(s) 1403
PspEI GGTNACC 1 cut(s) 3655
PspFI CCCAGC 1 cut(s) 1103
PspGI CCWGG 6 cut(s) 1168, 1273, 2313, 2802, 3025, 3618
PspLI CGTACG 1 cut(s) 17
PspN4I GGNNCC 5 cut(s) 308, 805, 2110, 2449, 3648
PspPI GGNCC 7 cut(s) 289, 307, 1193, 1245, 3050, 3102, 3647
PspPPI RGGWCCY 1 cut(s) 307
PstI CTGCAG 1 cut(s) 1378
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 4 cut(s) 1172, 3167, 3705, 3786
PvuII CAGCTG 3 cut(s) 104, 458, 1334
RseI CAYNNNNRTG 4 cut(s) 36, 363, 738, 915
SacI GAGCTC 1 cut(s) 1217
SapI GCTCTTC 1 cut(s) 2838
SatI GCNGC 8 cut(s) 102, 105, 267, 488, 880, 1968, 2187, 2818
Sau96I GGNCC 7 cut(s) 289, 307, 1193, 1245, 3050, 3102, 3647
ScaI AGTACT 1 cut(s) 2004
SchI GAGTC 3 cut(s) 2245, 2254, 3761
ScrFI CCNGG 6 cut(s) 1170, 1275, 2315, 2804, 3027, 3620
SduI GDGCHC 6 cut(s) 837, 1217, 1577, 1963, 2038, 2482
SexAI ACCWGGT 1 cut(s) 1273
SfcI CTRYAG 1 cut(s) 1374
SinI GGWCC 4 cut(s) 307, 1193, 3050, 3647
SmiMI CAYNNNNRTG 4 cut(s) 36, 363, 738, 915
SmlI CTYRAG 4 cut(s) 901, 2284, 2706, 3135
SmoI CTYRAG 4 cut(s) 901, 2284, 2706, 3135
SnaBI TACGTA 1 cut(s) 1754
SpeI ACTAGT 1 cut(s) 1669
SseBI AGGCCT 2 cut(s) 859, 1239
SsiI CCGC 9 cut(s) 487, 880, 1415, 1713, 1854, 1965, 2186, 3177, 3263
SspI AATATT 1 cut(s) 337
SspMI CTAG 9 cut(s) 1077, 1530, 1670, 1748, 2229, 2606, 2783, 3000, 3126
SstI GAGCTC 1 cut(s) 1217
StuI AGGCCT 2 cut(s) 859, 1239
StyD4I CCNGG 6 cut(s) 1168, 1273, 2313, 2802, 3025, 3618
StyI CCWWGG 4 cut(s) 410, 1248, 1698, 3746
TaaI ACNGT 8 cut(s) 811, 1457, 1819, 1933, 2017, 2116, 3574, 3688
TaiI ACGT 5 cut(s) 363, 1405, 1756, 3360, 3595
TaqI TCGA 7 cut(s) 119, 363, 965, 1348, 1624, 1838, 2069
TaqII GACCGA 1 cut(s) 2703
TatI WGTACW 1 cut(s) 2002
TauI GCSGC 3 cut(s) 490, 882, 2189
TfiI GAWTC 9 cut(s) 695, 775, 1608, 1742, 1850, 2008, 2489, 2537, 3120
TseFI GTSAC 6 cut(s) 181, 1152, 1585, 2641, 2774, 2982
TseI GCWGC 5 cut(s) 101, 104, 266, 1967, 2817
Tsp45I GTSAC 6 cut(s) 181, 1152, 1585, 2641, 2774, 2982
TspGWI ACGGA 2 cut(s) 1926, 3162
Van91I CCANNNNNTGG 2 cut(s) 943, 3619
Vha464I CTTAAG 1 cut(s) 901
VneI GTGCAC 1 cut(s) 1573
VpaK11BI GGWCC 4 cut(s) 307, 1193, 3050, 3647
VspI ATTAAT 3 cut(s) 590, 1013, 2696
XapI RAATTY 7 cut(s) 1097, 1302, 1553, 2408, 2798, 3302, 3485
XbaI TCTAGA 2 cut(s) 2605, 2782
XceI RCATGY 6 cut(s) 35, 2372, 2726, 3015, 3213, 3334
XmnI GAANNNNTTC 1 cut(s) 3070
XspI CTAG 9 cut(s) 1077, 1530, 1670, 1748, 2229, 2606, 2783, 3000, 3126
ZrmI AGTACT 1 cut(s) 2004
Zsp2I ATGCAT 4 cut(s) 1646, 2299, 2466, 2728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.