MD02G1255200.v1.1

ABC transporter D family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006235
Physical Location & Seq
Reverse (-)
30709065 .. 30723126
14062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1255200.v1.1.491

Sequence Viewer

Length: 4239 bp
ATGCCGTCGCTTCAGCTGCTGCAATTGACTGAGCATGGCCGCAATTTCATGGCGTCGAGGAGGAAAACTTTACTACTTGCCACTGGTATCATAGTTGCTGGTGGGACGGCTGCATATGTTCAATCGCGATTAAACCATAAAAAGCATGGTTTTCATGGTCATTACAATGGGCTTGATGATAGTGAAGAGACAACGGAGAGGGCCATGTTGAGTGATCACAAGTTAAAGAAACCTCCACGTAAGAAAGGGGGATTGAAGTCGCTTCAAGTCCTTGCTGCAATTCTCTTATCCGAGATGGGTCAAATGGGTGTGAGGGATCTTTTATCTTTACTCTCCATAGTGGTGTTGCGAACTGCTTTGAGCAACAGACTAGCAAAAGTCCAAGGATTTCTATTCCGTGCAGCTTTCCTTCGACGCGTGCCATTATTTTTCCGGTTGATTTCTGAAAATATTTTACTATGTTTTCTTGTATCAACTATGCATTCTACTTCAAAGTACATCACCGGAACCTTAAGTCTGCGATTCAGAAAAATATTGACAAAACTTATCCATTCCCATTATTTTGAGAATATCGCATACTACAAAATGTCTCATGTCGATGGTCGAATAACAAATCCTGAACAGCGAATTGCAAGTGATGTGCCAAAGTTCTGTTCAGAGTTGAGTGAAATTGTGCAGGATGATTTGACAGCTGTTACTGATGGTGTACTTTATACTTGGCGGTTATGCTCTTATGCCAGTCCTAAATATGTATTCTGGATATTGGCATATGTATTAGGGGCAGGGGCCATGATAAGAAATTTTTCTCCTGCTTTTGGGAAACTAATGTCCAAAGAGCAACAATTGGAAGGGGAGTACCGGCAGCTTCATTCTCGATTAAGGACCCATGCAGAAAGCGTGGCATTTTATGGTGGGGAGAATAGAGAAGAGTCTCATATTAAGAAGAAGTTTGAGACACTGATTAGGCATATGCGTGTTGTCCTTCATGACCATTGGTGGTTTGGCATGATTCAAGACTTTCTATTGAAGTATCTTGGTGCTACAGTTGCTGTTATACTGATTATTGAGCCTTTCTTTTCGGGACATCTGAGGCCTGACACTTCAACTTTAGGTCGCGCAGAGATGTTAAGCAATTTAAGATATCATACTAGTGTCATAATTTCACTATTTCAGTCTCTGGGAACTCTTTCAATAAGTTCAAGAAAGCTAAATCGGCTCAGTGGTTACGCCGATCGCATTCATGAATTATTGGCCATATCAAAAGAGCTCAGTGTGGCTAATAGTAAATCTTCCGGGACCAGAAATTGCTTTAGTGAAGCTGATTACATTGAATTTTCTGGTGTCAAGGTTGTTACGCCTACGGGTAATGTTTTGGTGGATAATTTGTCACTTAGGGTCGAATCAGGATCTAATCTTTTGATTACAGGTAAGCAGTGTCCCTCAAAGTTATTTTCATGTGGTGCAACTTCGTTCTATTTGTACTTGTCTCCTGTCTCTCTTTGCTGTCATTTCATTTGTATCTTTTTTGTTTCTTGCCACCATCTATTCTATTGCATTATCAAGTGGAAAAGAAAATATCAGTTTTACGTGATATTGTTTTTGTATGGAGTTTGGCAGCCTCTCCACTTTGAAACATGGATTTTTAGTACTATATTAGGTCCAAACGGTAGTGGGAAGAGCTCACTTTTCCGAGTTCTAGGAGGGCTCTGGCCCTTGGTATCTGGCCATATTGTGAAACCCGGTGTTGGAACTGATCTCAATAAAGAAATTTTCTATGTACCACAAAGACCATACACAGCTGTTGGAACACTACGGGATCAGTTGATTTATCCTCTTACTGCAGATCAAGGGGTTGAACCACTCACACGCAGTGGAATGGTGGAGTTATTGAGAAATGTTGACCTTGAGTATTTACTAGATCGGTATCCACCTGAGAAAGAGATAAATTGGGGTGATGAATTATCTCTTGGGGAGCAACAAAGATTGGGGATGGCTAGACTTTTCTATCACAAGCCTAAATTTGCAATTCTTGATGAGTGCACTAGTGCTGTCACAACTGACATGGAGGAACGTTTTTGTGCTAAAGTTCGAGCAATGGGAACGTCATGCATTACTATATCCCACCGTCCAGCACTTGTTGCATTTCATGATGTGGTGTTGTCCTTGGATGGTGAAGGTGGTTGGAGTGTTCAGGTCAAAAGGGAGGATTCTCCTCTCCTTAATGAAGGTGGGAGAAATATGATGTCTGAGTCAAACAGGCAGAGTGATGCAATGACAGTTCAACGTGCATTCACCACACCTAAAAAGGTTTCCACAATCTCAAATTCTTATATTGGTGAGGTGATAGCAGTGTCTCCTCCTGAGGATCAGTTTGGTATACTTCCATTTGTTCCACAGCTCCAAGGGGCACCTCGAGCTTTGCCAGTGAGAGTAGCAGCCATGTTCAAAGTTCTGATCCCCACAGTCCTTGATAAACAAGGAGCACAACTGCTTGCAGTTGCTCTTCTTGTGGTATCGAGAACATGGATTTCAGATCGTATTGCTTCATTAAACGGAACCACTGTGAAATTTGTTTTAGAGCAGGATAAGGCAGCCTTTATTCGTCTAATTGGCGTTAGTGTGGTTCAGAGTGCTGCGTCATCCTTCATTGCACCTTCTTTAAGGCACTTGACAGCCAGGCTGGCTCTTGGGTGGAGGATCCGTTTAACTCAACATTTACTCAAGAACTACTTGAGAAATAATGCATTTTACAAGGTCTTCCACATGTCAAGCAAGAAGATAGATGCAGATCAGAGAATTACTCAGGACCTAGAAAAGTTAACCTCTGACTTGTCGGGACTGGTTACAGGAATGGTAAAGCCATCTGTGGACATTCTCTGGTTCACCTGGAGAATGAAGCTTTTGACGGGGCGGAGGGGAGTTGCTATATTATATGCTTATATGTTACTAGGTCTAGGTTTTTTGAGGGCTGTTACCCCTGAATTTGGTGATTTGGTGAGTCGAGAACAACAGCTTGAAGGAACATTTAGGTTTATGCATGAAAGATTGCGTGCTCATGCCGAATCTGTTGCTTTCTTTGGAGGTGGTTCTAGAGAAAAAGCTATGGTTGAATCTAAGTTCAAGGAGCTTCTTGATCACTCCTCCTCTCTTTTGAAGAAGAAGTGGTTGTTTGGTATTCTTGATGATTTTACCACAAAACAACTCCCACATAATGTGACGTGGGGGTTGAGCTTGTTATATGCCATGGAACACAAGGGAGATCGAGCATTAATATCCACTCAAGGTGAGCTGGCACATGCATTGCGTTTCTTAGCATCTGTTGTATCTCAAAGCTTTTTAGCTTTTGGCGACATTCTGGAATTGCATAGAAAGTTCCTTGAACTGTCTGGCGGCATCAACAGAATTTTTGAGCTTGAAGAGCTCCTGGATGTTGCACAATCTGTTGTTGGCTATTCTGGGGCTTCCGAAACTGTTACTCTGTCACCATCTCAGGGGAGAGATTTTCATTCTGAAGATGCCATTACCTTTTCTGAAGTCAATATTATCACCCCATCACAGAAAATGTTGGCCAGGAAGTTAACATGTGATATAGTACCTGGGAAAAGCTTGCTTGTTACTGGTCCAAATGGGAGTGGAAAAAGTTCTGTCTTTAGAGTTCTTAGAGGTCTTTGGCCCATCACGAGTGGAAGAATCACTCAACCGTCACAACATGTTACGGAAGACATTGGCTCTGGTTGTGGAGTGTTTTATGTTCCTCAGCGACCGTATACATGTTTGGGAACACTGAGGGATCAAATTATATATCCTCTTTCTTGTGATGAGGCTGAGTTAAGGGCACTGAAGTTGTATCGAGAAGGTGAAGAAATCTCTGACAACACCAATATTCTAGACATGCGTTTGAGAACTATTCTGGAGAATGTTCGATTGAGTTATCTTTTGGAAAGGGAGGAAGGTGGTTGGGATGCGAATCTGAATTGGGAGGACACTCTCTCTCTTGGCGAACAGCAGAGATTGGGCATGGCACGCTTATTTTTCCACAAGCCTAAATTTGCCATCCTTGATGAATGCACCAATGCTACGAGCGTTGATGTCGAAGAACAACTTTACCGGCTTGCAAAAGACATGAATATCACAGTTGTTACCTCATCACAACGGCCTGCTCTAATACCGTTCCATTCCCTGGAATTACGGTTAATTGATGGTGAGGGTAACTGGGAACTACGTTCTATAAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006629 GO:0008150 GO:0008152 GO:0009056 GO:0016042 GO:0044238 GO:0071704 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko02010 ko04146 map02010 map04146
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

1413

Amino Acids

158.9

Weight (kDa)

9.1

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 91 - 359 3.3e-87 ABC transporter transmembrane region 2
ABC_tran PF00005 550 - 682 2.1e-15 ABC transporter
ABC_membrane_2 PF06472 814 - 1080 1.5e-83 ABC transporter transmembrane region 2
ABC_tran PF00005 1192 - 1358 4.3e-16 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2407
AccB7I CCANNNNNTGG 1 cut(s) 4180
AccI GTMKAC 2 cut(s) 2377, 3767
AccII CGCG 3 cut(s) 127, 417, 1116
AciI CCGC 4 cut(s) 40, 721, 2911, 3390
AclI AACGTT 1 cut(s) 2071
AclWI GGATC 8 cut(s) 324, 1414, 1824, 2373, 2449, 2692, 2705, 3798
AcoI YGGCCR 4 cut(s) 37, 1251, 1723, 3567
AcsI RAATTY 9 cut(s) 799, 1331, 1767, 2018, 2323, 2567, 2981, 3402, 4046
AcuI CTGAAG 3 cut(s) 3531, 3552, 3860
AcyI GRCGYC 1 cut(s) 53
AdeI CACNNNGTG 1 cut(s) 1871
AfaI GTAC 7 cut(s) 497, 708, 857, 1481, 1648, 1779, 3594
AfiI CCNNNNNNNGG 5 cut(s) 815, 1745, 2984, 3491, 4180
AflII CTTAAG 1 cut(s) 511
AflIII ACRYGT 5 cut(s) 415, 2763, 3581, 3709, 3770
AhlI ACTAGT 2 cut(s) 1148, 2042
AjiI CACGTC 1 cut(s) 3219
AjnI CCWGG 6 cut(s) 2675, 2885, 3423, 3569, 3595, 4179
AjuI GAANNNNNNNTTGG 4 cut(s) 637, 669, 1950, 1982
Alw21I GWGCWC 6 cut(s) 1269, 1682, 2042, 2485, 3055, 3423
Alw26I GTCTC 8 cut(s) 182, 594, 936, 947, 1179, 1491, 1498, 2358
Alw44I GTGCAC 1 cut(s) 2038
AlwI GGATC 8 cut(s) 324, 1414, 1824, 2373, 2449, 2692, 2705, 3798
AlwNI CAGNNNCTG 3 cut(s) 19, 1049, 1177
Ama87I CYCGRG 1 cut(s) 2412
ApaLI GTGCAC 1 cut(s) 2038
ApoI RAATTY 9 cut(s) 799, 1331, 1767, 2018, 2323, 2567, 2981, 3402, 4046
AseI ATTAAT 1 cut(s) 3269
Asp700I GAANNNNTTC 3 cut(s) 802, 1186, 3640
AspLEI GCGC 1 cut(s) 1118
AspS9I GGNCC 9 cut(s) 201, 786, 882, 1296, 1658, 1710, 2806, 3620, 3672
AsuC2I CCSGG 2 cut(s) 1294, 1740
AvaI CYCGRG 1 cut(s) 2412
AvaII GGWCC 5 cut(s) 882, 1296, 1658, 2806, 3620
AxyI CCTNAGG 1 cut(s) 2361
BaeGI GKGCMC 3 cut(s) 2042, 2410, 3838
BalI TGGCCA 3 cut(s) 1253, 1725, 3569
BamHI GGATCC 1 cut(s) 2697
BanI GGYRCC 1 cut(s) 2407
BanII GRGCYC 4 cut(s) 1269, 1682, 1707, 3423
BauI CACGAG 1 cut(s) 3679
BbsI GAAGAC 2 cut(s) 2749, 3726
Bbv12I GWGCWC 6 cut(s) 1269, 1682, 2042, 2485, 3055, 3423
BbvCI CCTCAGC 1 cut(s) 3756
BceAI ACGGC 2 cut(s) 123, 4169
BcgI CGANNNNNNTGC 2 cut(s) 3050, 3084
BciT130I CCWGG 6 cut(s) 2677, 2887, 3425, 3571, 3597, 4181
BciVI GTATCC 1 cut(s) 1935
BclI TGATCA 2 cut(s) 214, 3133
BcnI CCSGG 2 cut(s) 1294, 1740
BcoDI GTCTC 8 cut(s) 182, 594, 936, 947, 1179, 1491, 1498, 2358
BcuI ACTAGT 2 cut(s) 1148, 2042
BfmI CTRYAG 2 cut(s) 1041, 1839
BfrI CTTAAG 1 cut(s) 511
BfuI GTATCC 1 cut(s) 1935
BglI GCCNNNNNGGC 1 cut(s) 2681
BmcAI AGTACT 1 cut(s) 1648
Bme1390I CCNGG 8 cut(s) 1294, 1740, 2677, 2887, 3425, 3571, 3597, 4181
Bme18I GGWCC 5 cut(s) 882, 1296, 1658, 2806, 3620
BmeT110I CYCGRG 1 cut(s) 2412
BmgBI CACGTC 1 cut(s) 3219
BmgT120I GGNCC 9 cut(s) 201, 786, 882, 1296, 1658, 1710, 2806, 3620, 3672
BmiI GGNNCC 7 cut(s) 508, 787, 884, 1297, 2409, 2557, 2699
BmrFI CCNGG 8 cut(s) 1294, 1740, 2677, 2887, 3425, 3571, 3597, 4181
BmrI ACTGGG 1 cut(s) 4222
BmsI GCATC 6 cut(s) 2257, 2773, 3323, 3402, 3505, 3952
BmuI ACTGGG 1 cut(s) 4222
BpiI GAAGAC 2 cut(s) 2749, 3726
BplI GAGNNNNNCTC 2 cut(s) 2785, 2817
BpmI CTGGAG 2 cut(s) 2908, 3932
Bpu10I CCTNAGC 1 cut(s) 3756
BpuEI CTTGAG 4 cut(s) 1925, 2705, 2752, 3264
BpuMI CCSGG 2 cut(s) 1294, 1740
BsaAI YACGTR 2 cut(s) 239, 1588
BsaBI GATNNNNATC 3 cut(s) 1923, 2787, 3966
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 7 cut(s) 382, 1713, 2163, 2401, 3243, 3596, 4179
BsaWI WCCGGW 2 cut(s) 432, 503
BsaXI ACNNNNNCTCC 2 cut(s) 2686, 2716
Bsc4I CCNNNNNNNGG 5 cut(s) 815, 1745, 2984, 3491, 4180
Bse118I RCCGGY 2 cut(s) 858, 4107
Bse1I ACTGG 6 cut(s) 88, 738, 2423, 2844, 3622, 4217
Bse21I CCTNAGG 1 cut(s) 2361
Bse3DI GCAATG 4 cut(s) 2100, 2277, 2646, 3299
Bse8I GATNNNNATC 3 cut(s) 1923, 2787, 3966
BseBI CCWGG 6 cut(s) 2677, 2887, 3425, 3571, 3597, 4181
BseDI CCNNGG 7 cut(s) 382, 1713, 2163, 2401, 3243, 3596, 4179
BseGI GGATG 7 cut(s) 685, 1995, 2173, 2639, 3433, 3967, 4053
BseJI GATNNNNATC 3 cut(s) 1923, 2787, 3966
BseLI CCNNNNNNNGG 5 cut(s) 815, 1745, 2984, 3491, 4180
BseMI GCAATG 4 cut(s) 2100, 2277, 2646, 3299
BseNI ACTGG 6 cut(s) 88, 738, 2423, 2844, 3622, 4217
BseRI GAGGAG 5 cut(s) 73, 2202, 2346, 3130, 3133
BseSI GKGCMC 3 cut(s) 2042, 2410, 3838
BsgI GTGCAG 2 cut(s) 420, 695
Bsh1236I CGCG 3 cut(s) 127, 417, 1116
Bsh1285I CGRYCG 2 cut(s) 1234, 3764
BshNI GGYRCC 1 cut(s) 2407
BsiEI CGRYCG 2 cut(s) 1234, 3764
BsiHKAI GWGCWC 6 cut(s) 1269, 1682, 2042, 2485, 3055, 3423
BsiHKCI CYCGRG 1 cut(s) 2412
BsiSI CCGG 6 cut(s) 433, 504, 859, 1293, 1740, 4108
BslFI GGGAC 5 cut(s) 118, 1095, 1309, 1422, 2850
BslI CCNNNNNNNGG 5 cut(s) 815, 1745, 2984, 3491, 4180
BsmAI GTCTC 8 cut(s) 182, 594, 936, 947, 1179, 1491, 1498, 2358
BsmFI GGGAC 5 cut(s) 118, 1095, 1309, 1422, 2850
BsmI GAATGC 4 cut(s) 481, 1236, 2288, 4070
BsoBI CYCGRG 1 cut(s) 2412
Bsp1286I GDGCHC 9 cut(s) 1269, 1682, 1707, 2042, 2410, 2485, 3055, 3423, 3838
Bsp19I CCATGG 1 cut(s) 3243
Bsp68I TCGCGA 1 cut(s) 127
BspACI CCGC 4 cut(s) 40, 721, 2911, 3390
BspFNI CGCG 3 cut(s) 127, 417, 1116
BspHI TCATGA 3 cut(s) 985, 1240, 2146
BspLI GGNNCC 7 cut(s) 508, 787, 884, 1297, 2409, 2557, 2699
BspMAI CTGCAG 1 cut(s) 1843
BspPI GGATC 8 cut(s) 324, 1414, 1824, 2373, 2449, 2692, 2705, 3798
BspQI GCTCTTC 3 cut(s) 1670, 2508, 3411
BspT107I GGYRCC 1 cut(s) 2407
BspTI CTTAAG 1 cut(s) 511
BsrDI GCAATG 4 cut(s) 2100, 2277, 2646, 3299
BsrFI RCCGGY 2 cut(s) 858, 4107
BsrI ACTGG 6 cut(s) 88, 738, 2423, 2844, 3622, 4217
BssAI RCCGGY 2 cut(s) 858, 4107
BssECI CCNNGG 7 cut(s) 382, 1713, 2163, 2401, 3243, 3596, 4179
BssNAI GTATAC 2 cut(s) 2378, 3768
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 1 cut(s) 3679
BssT1I CCWWGG 5 cut(s) 382, 1713, 2163, 2401, 3243
Bst1107I GTATAC 2 cut(s) 2378, 3768
Bst2BI CACGAG 1 cut(s) 3679
Bst2UI CCWGG 6 cut(s) 2677, 2887, 3425, 3571, 3597, 4181
Bst6I CTCTTC 5 cut(s) 180, 921, 1670, 2508, 3411
BstACI GRCGYC 1 cut(s) 53
BstAFI CTTAAG 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 2138
BstBAI YACGTR 2 cut(s) 239, 1588
BstC8I GCNNGC 9 cut(s) 419, 2493, 2682, 3051, 3291, 3608, 4024, 4113, 4158
BstDSI CCRYGG 1 cut(s) 3243
BstF5I GGATG 7 cut(s) 685, 1995, 2173, 2639, 3433, 3967, 4053
BstFNI CGCG 3 cut(s) 127, 417, 1116
BstHHI GCGC 1 cut(s) 1118
BstMAI GTCTC 8 cut(s) 182, 594, 936, 947, 1179, 1491, 1498, 2358
BstMCI CGRYCG 2 cut(s) 1234, 3764
BstMWI GCNNNNNNNGC 8 cut(s) 16, 1046, 1213, 2138, 2414, 2681, 3418, 4023
BstNI CCWGG 6 cut(s) 2677, 2887, 3425, 3571, 3597, 4181
BstNSI RCATGY 6 cut(s) 2767, 3299, 3585, 3713, 3774, 3895
BstSCI CCNGG 8 cut(s) 1292, 1738, 2675, 2885, 3423, 3569, 3595, 4179
BstSFI CTRYAG 2 cut(s) 1041, 1839
BstSLI GKGCMC 3 cut(s) 2042, 2410, 3838
BstUI CGCG 3 cut(s) 127, 417, 1116
BstV2I GAAGAC 2 cut(s) 2749, 3726
BstX2I RGATCY 3 cut(s) 316, 1406, 2697
BstYI RGATCY 3 cut(s) 316, 1406, 2697
BstZ17I GTATAC 2 cut(s) 2378, 3768
Bsu36I CCTNAGG 1 cut(s) 2361
BsuI GTATCC 1 cut(s) 1935
BtgI CCRYGG 1 cut(s) 3243
BtrI CACGTC 1 cut(s) 3219
BtsCI GGATG 7 cut(s) 685, 1995, 2173, 2639, 3433, 3967, 4053
BtsI GCAGTG 3 cut(s) 1439, 1876, 2355
BtuMI TCGCGA 1 cut(s) 127
Cac8I GCNNGC 9 cut(s) 419, 2493, 2682, 3051, 3291, 3608, 4024, 4113, 4158
CaiI CAGNNNCTG 3 cut(s) 19, 1049, 1177
CciI TCATGA 3 cut(s) 985, 1240, 2146
CfoI GCGC 1 cut(s) 1118
Cfr10I RCCGGY 2 cut(s) 858, 4107
Cfr13I GGNCC 9 cut(s) 201, 786, 882, 1296, 1658, 1710, 2806, 3620, 3672
CseI GACGC 3 cut(s) 42, 423, 2625
Csp6I GTAC 7 cut(s) 496, 707, 856, 1480, 1647, 1778, 3593
CspCI CAANNNNNGTGG 2 cut(s) 2449, 2484
CviQI GTAC 7 cut(s) 496, 707, 856, 1480, 1647, 1778, 3593
DraIII CACNNNGTG 1 cut(s) 1871
EaeI YGGCCR 4 cut(s) 37, 1251, 1723, 3567
Eam1104I CTCTTC 5 cut(s) 180, 921, 1670, 2508, 3411
EarI CTCTTC 5 cut(s) 180, 921, 1670, 2508, 3411
EciI GGCGGA 1 cut(s) 2926
Ecl136II GAGCTC 3 cut(s) 1267, 1680, 3421
Eco130I CCWWGG 5 cut(s) 382, 1713, 2163, 2401, 3243
Eco147I AGGCCT 1 cut(s) 1093
Eco24I GRGCYC 4 cut(s) 1269, 1682, 1707, 3423
Eco32I GATATC 1 cut(s) 1142
Eco47I GGWCC 5 cut(s) 882, 1296, 1658, 2806, 3620
Eco53kI GAGCTC 3 cut(s) 1267, 1680, 3421
Eco57I CTGAAG 3 cut(s) 3531, 3552, 3860
Eco81I CCTNAGG 1 cut(s) 2361
Eco88I CYCGRG 1 cut(s) 2412
EcoICRI GAGCTC 3 cut(s) 1267, 1680, 3421
EcoO109I RGGNCCY 2 cut(s) 882, 2806
EcoRII CCWGG 6 cut(s) 2675, 2885, 3423, 3569, 3595, 4179
EcoRV GATATC 1 cut(s) 1142
EcoT14I CCWWGG 5 cut(s) 382, 1713, 2163, 2401, 3243
EcoT22I ATGCAT 5 cut(s) 483, 2111, 2746, 3039, 3301
EcoT38I GRGCYC 4 cut(s) 1269, 1682, 1707, 3423
ErhI CCWWGG 5 cut(s) 382, 1713, 2163, 2401, 3243
FalI AAGNNNNNCTT 6 cut(s) 2579, 2611, 2714, 2746, 4086, 4118
FaqI GGGAC 5 cut(s) 118, 1095, 1309, 1422, 2850
FauNDI CATATG 3 cut(s) 115, 769, 969
FbaI TGATCA 2 cut(s) 214, 3133
FblI GTMKAC 2 cut(s) 2377, 3767
FokI GGATG 7 cut(s) 692, 2002, 2180, 2626, 3440, 3974, 4040
FriOI GRGCYC 4 cut(s) 1269, 1682, 1707, 3423
GlaI GCGC 1 cut(s) 1117
GsuI CTGGAG 2 cut(s) 2908, 3932
HapII CCGG 6 cut(s) 433, 504, 859, 1293, 1740, 4108
HgaI GACGC 3 cut(s) 42, 423, 2625
HhaI GCGC 1 cut(s) 1118
Hin1I GRCGYC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 1116
HinP1I GCGC 1 cut(s) 1116
HincII GTYRAC 3 cut(s) 1900, 2820, 3579
HindII GTYRAC 3 cut(s) 1900, 2820, 3579
HindIII AAGCTT 3 cut(s) 2897, 3331, 3604
HpaI GTTAAC 2 cut(s) 2820, 3579
HpaII CCGG 6 cut(s) 433, 504, 859, 1293, 1740, 4108
Hpy166II GTNNAC 9 cut(s) 707, 1900, 2040, 2378, 2820, 2869, 2883, 3579, 3768
Hpy8I GTNNAC 9 cut(s) 707, 1900, 2040, 2378, 2820, 2869, 2883, 3579, 3768
Hpy99I CGWCG 3 cut(s) 10, 58, 417
HpyCH4IV ACGT 7 cut(s) 238, 1587, 2071, 2102, 2284, 3218, 4222
HpyF10VI GCNNNNNNNGC 8 cut(s) 16, 1046, 1213, 2138, 2414, 2681, 3418, 4023
HpySE526I ACGT 7 cut(s) 238, 1587, 2071, 2102, 2284, 3218, 4222
Hsp92I GRCGYC 1 cut(s) 53
HspAI GCGC 1 cut(s) 1116
Ksp22I TGATCA 2 cut(s) 214, 3133
KspAI GTTAAC 2 cut(s) 2820, 3579
LguI GCTCTTC 3 cut(s) 1670, 2508, 3411
LmnI GCTCC 5 cut(s) 1972, 2403, 2480, 3124, 3426
LweI GCATC 6 cut(s) 2257, 2773, 3323, 3402, 3505, 3952
MaeII ACGT 7 cut(s) 238, 1587, 2071, 2102, 2284, 3218, 4222
MfeI CAATTG 2 cut(s) 23, 842
MflI RGATCY 3 cut(s) 316, 1406, 2697
MhlI GDGCHC 9 cut(s) 1269, 1682, 1707, 2042, 2410, 2485, 3055, 3423, 3838
MlsI TGGCCA 3 cut(s) 1253, 1725, 3569
MluI ACGCGT 1 cut(s) 415
MluNI TGGCCA 3 cut(s) 1253, 1725, 3569
MlyI GAGTC 3 cut(s) 938, 2258, 3007
MmeI TCCRAC 3 cut(s) 1726, 1783, 2162
Mox20I TGGCCA 3 cut(s) 1253, 1725, 3569
Mph1103I ATGCAT 5 cut(s) 483, 2111, 2746, 3039, 3301
MroXI GAANNNNTTC 3 cut(s) 802, 1186, 3640
MscI TGGCCA 3 cut(s) 1253, 1725, 3569
MslI CAYNNNNRTG 5 cut(s) 165, 341, 597, 972, 1149
Msp20I TGGCCA 3 cut(s) 1253, 1725, 3569
MspA1I CMGCKG 3 cut(s) 16, 692, 1799
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 6 cut(s) 433, 504, 859, 1293, 1740, 4108
MspR9I CCNGG 8 cut(s) 1294, 1740, 2677, 2887, 3425, 3571, 3597, 4181
MunI CAATTG 2 cut(s) 23, 842
Mva1269I GAATGC 4 cut(s) 481, 1236, 2288, 4070
MvaI CCWGG 6 cut(s) 2677, 2887, 3425, 3571, 3597, 4181
MvnI CGCG 3 cut(s) 127, 417, 1116
MwoI GCNNNNNNNGC 8 cut(s) 16, 1046, 1213, 2138, 2414, 2681, 3418, 4023
NciI CCSGG 2 cut(s) 1294, 1740
NcoI CCATGG 1 cut(s) 3243
NdeI CATATG 3 cut(s) 115, 769, 969
NlaIV GGNNCC 7 cut(s) 508, 787, 884, 1297, 2409, 2557, 2699
NmuCI GTSAC 5 cut(s) 1386, 2050, 3214, 3480, 3702
NruI TCGCGA 1 cut(s) 127
NsiI ATGCAT 5 cut(s) 483, 2111, 2746, 3039, 3301
NspI RCATGY 6 cut(s) 2767, 3299, 3585, 3713, 3774, 3895
PaeR7I CTCGAG 1 cut(s) 2412
PagI TCATGA 3 cut(s) 985, 1240, 2146
PceI AGGCCT 1 cut(s) 1093
PciI ACATGT 4 cut(s) 2763, 3581, 3709, 3770
PciSI GCTCTTC 3 cut(s) 1670, 2508, 3411
PctI GAATGC 4 cut(s) 481, 1236, 2288, 4070
PdmI GAANNNNTTC 3 cut(s) 802, 1186, 3640
PfeI GAWTC 8 cut(s) 522, 1009, 1400, 2207, 3062, 3110, 3690, 3967
PflMI CCANNNNNTGG 1 cut(s) 4180
PfoI TCCNGGA 2 cut(s) 1292, 3423
Ple19I CGATCG 1 cut(s) 1234
PleI GAGTC 3 cut(s) 937, 2257, 3006
PpsI GAGTC 3 cut(s) 937, 2257, 3006
Ppu21I YACGTR 2 cut(s) 239, 1588
PpuMI RGGWCCY 2 cut(s) 882, 2806
PscI ACATGT 4 cut(s) 2763, 3581, 3709, 3770
PshBI ATTAAT 1 cut(s) 3269
Psp124BI GAGCTC 3 cut(s) 1269, 1682, 3423
Psp1406I AACGTT 1 cut(s) 2071
Psp5II RGGWCCY 2 cut(s) 882, 2806
Psp6I CCWGG 6 cut(s) 2675, 2885, 3423, 3569, 3595, 4179
PspGI CCWGG 6 cut(s) 2675, 2885, 3423, 3569, 3595, 4179
PspN4I GGNNCC 7 cut(s) 508, 787, 884, 1297, 2409, 2557, 2699
PspPI GGNCC 9 cut(s) 201, 786, 882, 1296, 1658, 1710, 2806, 3620, 3672
PspPPI RGGWCCY 2 cut(s) 882, 2806
PspXI VCTCGAGB 1 cut(s) 2412
PstI CTGCAG 1 cut(s) 1843
PstNI CAGNNNCTG 3 cut(s) 19, 1049, 1177
PsuI RGATCY 3 cut(s) 316, 1406, 2697
PvuI CGATCG 1 cut(s) 1234
PvuII CAGCTG 3 cut(s) 16, 692, 1799
RruI TCGCGA 1 cut(s) 127
RsaI GTAC 7 cut(s) 497, 708, 857, 1481, 1648, 1779, 3594
RsaNI GTAC 7 cut(s) 496, 707, 856, 1480, 1647, 1778, 3593
RseI CAYNNNNRTG 5 cut(s) 165, 341, 597, 972, 1149
SacI GAGCTC 3 cut(s) 1269, 1682, 3423
SapI GCTCTTC 3 cut(s) 1670, 2508, 3411
Sau96I GGNCC 9 cut(s) 201, 786, 882, 1296, 1658, 1710, 2806, 3620, 3672
ScaI AGTACT 1 cut(s) 1648
SchI GAGTC 3 cut(s) 938, 2258, 3007
ScrFI CCNGG 8 cut(s) 1294, 1740, 2677, 2887, 3425, 3571, 3597, 4181
SduI GDGCHC 9 cut(s) 1269, 1682, 1707, 2042, 2410, 2485, 3055, 3423, 3838
SfaNI GCATC 6 cut(s) 2257, 2773, 3323, 3402, 3505, 3952
SfcI CTRYAG 2 cut(s) 1041, 1839
Sfr274I CTCGAG 1 cut(s) 2412
SinI GGWCC 5 cut(s) 882, 1296, 1658, 2806, 3620
SlaI CTCGAG 1 cut(s) 2412
SmiMI CAYNNNNRTG 5 cut(s) 165, 341, 597, 972, 1149
SmlI CTYRAG 6 cut(s) 511, 1904, 2412, 2720, 2731, 3279
SmoI CTYRAG 6 cut(s) 511, 1904, 2412, 2720, 2731, 3279
SpeI ACTAGT 2 cut(s) 1148, 2042
SseBI AGGCCT 1 cut(s) 1093
SsiI CCGC 4 cut(s) 40, 721, 2911, 3390
SspI AATATT 4 cut(s) 451, 534, 3541, 3883
SstI GAGCTC 3 cut(s) 1269, 1682, 3423
StuI AGGCCT 1 cut(s) 1093
StyD4I CCNGG 8 cut(s) 1292, 1738, 2675, 2885, 3423, 3569, 3595, 4179
StyI CCWWGG 5 cut(s) 382, 1713, 2163, 2401, 3243
TaiI ACGT 7 cut(s) 241, 1590, 2074, 2105, 2287, 3221, 4225
TatI WGTACW 4 cut(s) 495, 706, 1479, 1646
TauI GCSGC 2 cut(s) 42, 3393
TfiI GAWTC 8 cut(s) 522, 1009, 1400, 2207, 3062, 3110, 3690, 3967
TseFI GTSAC 5 cut(s) 1386, 2050, 3214, 3480, 3702
Tsp45I GTSAC 5 cut(s) 1386, 2050, 3214, 3480, 3702
TspGWI ACGGA 5 cut(s) 209, 386, 2568, 2690, 3731
Van91I CCANNNNNTGG 1 cut(s) 4180
Vha464I CTTAAG 1 cut(s) 511
VneI GTGCAC 1 cut(s) 2038
VpaK11BI GGWCC 5 cut(s) 882, 1296, 1658, 2806, 3620
VspI ATTAAT 1 cut(s) 3269
XapI RAATTY 9 cut(s) 799, 1331, 1767, 2018, 2323, 2567, 2981, 3402, 4046
XbaI TCTAGA 2 cut(s) 3089, 3886
XceI RCATGY 6 cut(s) 2767, 3299, 3585, 3713, 3774, 3895
XcmI CCANNNNNNNNNTGG 2 cut(s) 143, 998
XhoI CTCGAG 1 cut(s) 2412
XmiI GTMKAC 2 cut(s) 2377, 3767
XmnI GAANNNNTTC 3 cut(s) 802, 1186, 3640
ZrmI AGTACT 1 cut(s) 1648
Zsp2I ATGCAT 5 cut(s) 483, 2111, 2746, 3039, 3301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.