MD07G1066800.v1.1

ABC transporter D family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006235
Physical Location & Seq
Forward (+)
6293610 .. 6307839
14230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1066800.v1.1.491

Sequence Viewer

Length: 4023 bp
ATGCCGTCGCTTCAGCTGCTGCAATTGACTAAGCATGGCCGCAATTTCATGGCGTCGAGGAGGAAAACTTTACTACTTGCCACTGGTATCGTAGTTGCTGGGGGGACGGCTGCATACGTTCAATCGCGACTAAACCATAAAAAACATGCTTTTCATGGTCATTACAATGGGCTTAATGATAGTGAAGAAACAACGGAGAAGGCCTTGTTGAACGATCACAAGTTAAAGAAACCTCCACGTAAGAAAGGGGGATTGAAGTCTCTTCATGTCCTTGCTGCAATTCTCTTATCTGAGATGGGGCAAATGGGTGTGAGGGATCTTTTATCTTTACTCTCCATAGTGGTGTTGCGAGCTGCTTTGAGCAACAGACTAGCAAAAGTCCAAGGATTTCTATTCCGTGCAGCTTTCCTTCGACGTGCGCCGTTATTTTTTCGGTTGATTTCTGAAAATATTTTATTATGTTTTCTTGTATCAACCATGCATTCTACTTCAAAGTACATCACAGGAACCTTAAGTCTGCGGTTCAGAAAAATATTGACAAAACTTATCCATTCCCATTATTTTGAGAATATTGCGTACTACAAAATGTCTCATGTTGATGGTCGCATAACAAATCCTGAACAGCGAATTGCAAGTGATGTGCCAAAGTTCTGTTCAGAGTTGAGTGAAATTGTACAGGATGATTTGACAGCTGTCACTGATGGTGTACTTTATACTTGGCGGTTATGTTCATATGCCAGTCCAAAATATGTCTTCTGGATATTGGCATATGTATTAGGGGCTGGGGCCATGATAAGAAATTTTTCTCCTGCTTTTGGGAAACTAATGTCCAAAGAACAGCAATTGGAAGGGGAGTACCGGCAGCTTCATTCTCGATTAAGGACCCATGCAGAAAGTGTGGCATTTTATGGTGGGGAGAATAGAGAAGAATCTCATATTAAGAAGAAGTTTGAGACACTGATTGGGCATATGCGTGTTGTCCTTCATGACCATTGGTGGTTTGGCATGATTCAAGATTTTCTATTGAAGTATCTTGGTGCTACAGTTGCTGTTATACTGATTATTGAGCCTTTCTTTTCGGGACATCTGAGGCCCGACACTTCAACTTTAGGCCGTGCAGAGATGTTAAGCAATTTAAGATATCATACTAGTGTCATAATCTCACTATTTCAGTCTTTGGGAACTCTTTCGATAAGTTCAAGAAAACTAAATCGGCTCAGTGGTTACGCAGATCGCATTCATGAATTATTGGCCATATCAAGAGAGTTAAGTGTGGCTAATAGTAAATCTTCTGGGACGAGAAATTGCTTTAGTCAAGCTGATTACATTGAATTTGCAGGTGTCAAGGTTGTCACGCCCACGGGTAATGTTTTGGTGGATAATTTGTCACTTAGGGTCGAATCAGGATCTAATCTTTTGATTACAGGTCCAAATGGTAGTGGGAAGAGCTCACTTTTCCGAGTTCTAGGAGGGCTCTGGCCCTTGGTATCTGGCCATATTGTGAAACCTGGTGTTGGAACTGATCTCAATAAAGAAATTTTCTATGTACCACAAAGACCATACACAGCTGTTGGAACACTACGGGATCAGTTGATTTATCCTCTTACTGCGGATGAAGAGGTTGAACCACTCACACGCAGTGGAATGGTGGAGTTATTGAGAAATGTTGACCTTGAGTATTTATTAGACCGGTATCCACCAGAGAAAGAGATAAATTGGGGTGATGAATTATCTCTCGGGGAGCAACAAAGACTGGGGATGGCTAGACTTTTTTATCATAAGCCTAAATTTGCAATTCTTGATGAGTGCACGAGTGCTGTCACAACTGACATGGAGGAACGTTTTTGTGCCAAAGTTCGAGCAATGGGAACGTCATGCATTACTATCTCCCATCGTCCAGCACTAGTTGCATTTCATGATGTGGTGTTGTCCTTGGATGGTGAAGGTGGTTGGAGTGTTCAGGTCAAAAGGGAGGATTCTTCTCTCCTTAATGAAGGTGGGAGAAACATGATGTTGTCTGAGTCAAACAGGCAGAGTGATGCAATGACAGTTCAACGTGCATTCACCACGATTAAAATGGATTCCACAATCTCAAATTCGAAGGCACAATCATACGTTGGTGAGGTGATAGCAGTGTCTCCTTCTAAGGATCAGTGTGCCATAGTTCCATTTGTTCCACAGCTCCAAAGGCCACCTCGAGCTTTGCCAGTGAGAATAGCAGCTATGTTCAAAGTACTGATCCCCACAGTCCTTGATAAACAAGGAGCACAACTGCTTGCAGTTGCTCTTCTCGTGGTATCGAGAACATGGATTTCAGATCGTATTGCTTCATTAAATGGAACCACTGTGAAATTTGTATTAGAGCAGGATAAGGCAGCCTTTATTCGTCTAATTGGCATTAGTGTGCTTCAGAGTGCTGCATCGTCCTTCATTGCACCTTCTTTAAGGCACTTGACAGCCAGGCTGGCTCTTGGGTGGAGGATCCGTTTAACTCAACATTTACTCAAGAACTACTTGAGAAATAATGCATTTTACAAGGTCTTCCACATGTCAAGTAAGAAGATAGATGCAGATCAGAGAATTACTCAGGACCTAGAAAAGTTAACCTCTGACTTGTCGGGACTGGTTACAGGAATGGTCAAGCCATCTGTGGACATTCTCTGGTTCACCTGGAGAATGAAGCTTTTGACGGGGCGGAGGGGAGTTGCTATATTATATGCTTATATGTTACTGGGTCTAGGTTTTCTGAGGGCTGTTACCCCTGAATTTGGTGATCTAGTGAGTCAAGAACAACAACTTGAAGGAACGTTCAGGTTTATGCATGAAAGATTGCGTGCTCATGCCGAGTCTGTTGCTTTCTTTGGAGGTGGTTCTAGAGAAAAAGCTATGGTTGAATCTAAGTTCAAGGAGCTTCTTGATCACTCCTCGTCTCTTTTGAAGAAGAAGTGGTTGTTTGGTATTCTGGATGATTTTACCACAAAACAACTTCCACATAATGTGACGTGGGGGCTGAGCTTGTTATATGCCATGGAACACAAGGGAGACCGAGCATTGATATCCACTCAAGGTGAGCTGGCACATGCATTGCGTTTCTTAGCATCTGTTGTATCTCAAAGCTTTTTAGCTTTCGGCGACATTCTGGAATTGCATAGGAAGTTCCTTGAACTGTCTGGCGGCATCAACAGAATTTTTGAGCTTGAAGAGCTTCTAGATGTTGCACAATCTGTTGATGGTTATTCAGGGGCTTCTGAAACTGTTACTCTGTCACCATCTAAGGGGAGAGATTTTCATTCTGAAGATGCCATTACCTTCTCTGAAGTTAATATTATCACCCCATCACAGAAAATGTTGGCCAGGAAGTTAACATGTGATATAGTACCTGGGAAAAGCTTGCTTGTTACTGGTCCAAATGGGAGTGGTAAAAGTTCTGTCTTTAGAGTTCTTAGAGGTCTTTGGCCCATCACGAGTGGAAGAATCTCTCAACCGTCGCAACATGTTAAGGAAGATATTGGTTCTGGTTGTGGAGTGTTTTATGTTCCTCAGCGACCGTATACATGTTTGGGAACACTCAGGGATCAAATTATCTATCCTCTATCTTGTGAGGAGGCTGAGTTAAGGGCATTGAAGTTGTATCGAGAAGGTGAAGAAATTTCTGACAACACCAATATTCTAGACATGCGTTTAAGAACTATTCTGGAGAATGTTCGATTGAGTTATCTTTTGGGAAGAGAGGAAGGTGGTTGGGATGCGAATCTGAATTGGGAGGACACGCTCTCTCTTGGGGAACAGCAGAGATTGGGCATGGCACGCTTATTTTTCCACAAGCCTAAATTTGCGATCCTTGATGAATGCACCAATGCTACGAGCGTTGATGTCGAAGAACAACTTTATCGGCTCGCGAAAGACATGAATATCACAGTTGTTACCTCCTCACAACGTCCTGCTCTAATACCATTCCATTCCCTGGAATTACGGTTCATTGATGGTGAGGGTAACTGGGAACTTCGTTCAATAAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006629 GO:0008150 GO:0008152 GO:0009056 GO:0016042 GO:0044238 GO:0071704 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko02010 ko04146 map02010 map04146
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

1341

Amino Acids

150.2

Weight (kDa)

9.19

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 91 - 359 1.1e-86 ABC transporter transmembrane region 2
ABC_tran PF00005 460 - 605 4.1e-19 ABC transporter
ABC_membrane_2 PF06472 742 - 1008 1.4e-83 ABC transporter transmembrane region 2
ABC_tran PF00005 1120 - 1286 7.7e-16 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1328
Acc36I ACCTGC 1 cut(s) 1328
AccB7I CCANNNNNTGG 1 cut(s) 3964
AccI GTMKAC 1 cut(s) 3551
AccII CGCG 2 cut(s) 127, 3899
AciI CCGC 6 cut(s) 40, 520, 721, 1610, 2695, 3174
AclI AACGTT 2 cut(s) 1840, 2807
AclWI GGATC 9 cut(s) 324, 1414, 1593, 2157, 2233, 2476, 2489, 3582, 3832
AcoI YGGCCR 4 cut(s) 37, 1251, 1492, 3351
AcuI CTGAAG 3 cut(s) 2393, 3315, 3336
AcyI GRCGYC 1 cut(s) 53
AdeI CACNNNGTG 1 cut(s) 1640
AfaI GTAC 8 cut(s) 497, 578, 675, 708, 857, 1548, 2235, 3378
AfiI CCNNNNNNNGG 5 cut(s) 815, 1514, 2768, 3275, 3964
AflII CTTAAG 1 cut(s) 511
AflIII ACRYGT 4 cut(s) 2547, 3365, 3493, 3554
AgeI ACCGGT 1 cut(s) 1689
AhlI ACTAGT 2 cut(s) 1148, 1903
AjiI CACGTC 2 cut(s) 416, 3003
AjnI CCWGG 6 cut(s) 1507, 2459, 2669, 3353, 3379, 3963
AjuI GAANNNNNNNTTGG 2 cut(s) 637, 669
AloI GAACNNNNNNTCC 2 cut(s) 3959, 3991
Alw21I GWGCWC 4 cut(s) 1451, 1811, 2269, 2839
Alw26I GTCTC 6 cut(s) 264, 594, 947, 2142, 2934, 3036
Alw44I GTGCAC 1 cut(s) 1807
AlwI GGATC 9 cut(s) 324, 1414, 1593, 2157, 2233, 2476, 2489, 3582, 3832
AlwNI CAGNNNCTG 2 cut(s) 19, 1049
Ama87I CYCGRG 2 cut(s) 1736, 2196
ApaLI GTGCAC 1 cut(s) 1807
AsiGI ACCGGT 1 cut(s) 1689
Asp700I GAANNNNTTC 3 cut(s) 802, 1186, 3204
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 8 cut(s) 786, 882, 1092, 1427, 1479, 2590, 3404, 3456
AsuII TTCGAA 1 cut(s) 2099
AvaI CYCGRG 2 cut(s) 1736, 2196
AvaII GGWCC 4 cut(s) 882, 1427, 2590, 3404
BaeGI GKGCMC 1 cut(s) 1811
BalI TGGCCA 3 cut(s) 1253, 1494, 3353
BamHI GGATCC 1 cut(s) 2481
BanII GRGCYC 2 cut(s) 1451, 1476
BauI CACGAG 3 cut(s) 1810, 2291, 3463
BbsI GAAGAC 2 cut(s) 745, 2533
Bbv12I GWGCWC 4 cut(s) 1451, 1811, 2269, 2839
BbvCI CCTCAGC 1 cut(s) 3540
BceAI ACGGC 3 cut(s) 123, 406, 1098
BcgI CGANNNNNNTGC 2 cut(s) 2834, 2868
BciT130I CCWGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
BciVI GTATCC 1 cut(s) 1704
BclI TGATCA 1 cut(s) 2917
BcoDI GTCTC 6 cut(s) 264, 594, 947, 2142, 2934, 3036
BcuI ACTAGT 2 cut(s) 1148, 1903
BfmI CTRYAG 1 cut(s) 1041
BfrI CTTAAG 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 1328
BfuI GTATCC 1 cut(s) 1704
BglI GCCNNNNNGGC 1 cut(s) 2465
BlpI GCTNAGC 1 cut(s) 3011
BmcAI AGTACT 1 cut(s) 2235
Bme1390I CCNGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
Bme18I GGWCC 4 cut(s) 882, 1427, 2590, 3404
BmeT110I CYCGRG 2 cut(s) 1736, 2196
BmgBI CACGTC 2 cut(s) 416, 3003
BmgT120I GGNCC 8 cut(s) 786, 882, 1092, 1427, 1479, 2590, 3404, 3456
BmiI GGNNCC 5 cut(s) 508, 787, 884, 2341, 2483
BmrFI CCNGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
BmrI ACTGGG 3 cut(s) 1763, 2741, 4006
BmsI GCATC 7 cut(s) 2029, 2429, 2557, 3107, 3186, 3289, 3736
BmuI ACTGGG 3 cut(s) 1763, 2741, 4006
BoxI GACNNNNGTC 1 cut(s) 692
BpiI GAAGAC 2 cut(s) 745, 2533
BplI GAGNNNNNCTC 2 cut(s) 2569, 2601
BpmI CTGGAG 2 cut(s) 2692, 3716
Bpu10I CCTNAGC 1 cut(s) 3540
Bpu1102I GCTNAGC 1 cut(s) 3011
Bpu14I TTCGAA 1 cut(s) 2099
BpuEI CTTGAG 4 cut(s) 1694, 2489, 2536, 3048
BsaAI YACGTR 1 cut(s) 239
BsaBI GATNNNNATC 2 cut(s) 2571, 3750
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 3036
BsaJI CCNNGG 7 cut(s) 382, 1359, 1482, 1932, 3027, 3380, 3963
BsaWI WCCGGW 1 cut(s) 1689
BsaXI ACNNNNNCTCC 4 cut(s) 2470, 2500, 3596, 3626
Bsc4I CCNNNNNNNGG 5 cut(s) 815, 1514, 2768, 3275, 3964
Bse118I RCCGGY 2 cut(s) 858, 1689
Bse1I ACTGG 8 cut(s) 88, 738, 1758, 2207, 2628, 2736, 3406, 4001
Bse3DI GCAATG 4 cut(s) 1869, 2049, 2430, 3083
Bse8I GATNNNNATC 2 cut(s) 2571, 3750
BseBI CCWGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
BseDI CCNNGG 7 cut(s) 382, 1359, 1482, 1932, 3027, 3380, 3963
BseGI GGATG 6 cut(s) 685, 1618, 1764, 1942, 2971, 3751
BseJI GATNNNNATC 2 cut(s) 2571, 3750
BseLI CCNNNNNNNGG 5 cut(s) 815, 1514, 2768, 3275, 3964
BseMI GCAATG 4 cut(s) 1869, 2049, 2430, 3083
BseNI ACTGG 8 cut(s) 88, 738, 1758, 2207, 2628, 2736, 3406, 4001
BseRI GAGGAG 4 cut(s) 73, 2914, 3617, 3919
BseSI GKGCMC 1 cut(s) 1811
BseYI CCCAGC 2 cut(s) 98, 782
BsgI GTGCAG 2 cut(s) 420, 1137
Bsh1236I CGCG 2 cut(s) 127, 3899
Bsh1285I CGRYCG 1 cut(s) 3548
BshTI ACCGGT 1 cut(s) 1689
BsiEI CGRYCG 1 cut(s) 3548
BsiHKAI GWGCWC 4 cut(s) 1451, 1811, 2269, 2839
BsiHKCI CYCGRG 2 cut(s) 1736, 2196
BsiSI CCGG 2 cut(s) 859, 1690
BslFI GGGAC 4 cut(s) 118, 1095, 1309, 2634
BslI CCNNNNNNNGG 5 cut(s) 815, 1514, 2768, 3275, 3964
BsmAI GTCTC 6 cut(s) 264, 594, 947, 2142, 2934, 3036
BsmBI CGTCTC 1 cut(s) 2934
BsmFI GGGAC 4 cut(s) 118, 1095, 1309, 2634
BsmI GAATGC 4 cut(s) 481, 1236, 2060, 3854
Bso31I GGTCTC 1 cut(s) 3036
BsoBI CYCGRG 2 cut(s) 1736, 2196
Bsp119I TTCGAA 1 cut(s) 2099
Bsp1286I GDGCHC 5 cut(s) 1451, 1476, 1811, 2269, 2839
Bsp1407I TGTACA 1 cut(s) 673
Bsp1720I GCTNAGC 1 cut(s) 3011
Bsp19I CCATGG 1 cut(s) 3027
Bsp68I TCGCGA 2 cut(s) 127, 3899
BspACI CCGC 6 cut(s) 40, 520, 721, 1610, 2695, 3174
BspFNI CGCG 2 cut(s) 127, 3899
BspHI TCATGA 3 cut(s) 985, 1240, 1915
BspLI GGNNCC 5 cut(s) 508, 787, 884, 2341, 2483
BspMI ACCTGC 1 cut(s) 1328
BspPI GGATC 9 cut(s) 324, 1414, 1593, 2157, 2233, 2476, 2489, 3582, 3832
BspQI GCTCTTC 3 cut(s) 1439, 2292, 3195
BspT104I TTCGAA 1 cut(s) 2099
BspTI CTTAAG 1 cut(s) 511
BspTNI GGTCTC 1 cut(s) 3036
BsrDI GCAATG 4 cut(s) 1869, 2049, 2430, 3083
BsrFI RCCGGY 2 cut(s) 858, 1689
BsrGI TGTACA 1 cut(s) 673
BsrI ACTGG 8 cut(s) 88, 738, 1758, 2207, 2628, 2736, 3406, 4001
BssAI RCCGGY 2 cut(s) 858, 1689
BssECI CCNNGG 7 cut(s) 382, 1359, 1482, 1932, 3027, 3380, 3963
BssNAI GTATAC 1 cut(s) 3552
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 3 cut(s) 1810, 2291, 3463
BssT1I CCWWGG 4 cut(s) 382, 1482, 1932, 3027
Bst1107I GTATAC 1 cut(s) 3552
Bst2BI CACGAG 3 cut(s) 1810, 2291, 3463
Bst2UI CCWGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
Bst6I CTCTTC 6 cut(s) 267, 1439, 1611, 2292, 3195, 3721
BstACI GRCGYC 1 cut(s) 53
BstAFI CTTAAG 1 cut(s) 511
BstAPI GCANNNNNTGC 1 cut(s) 1907
BstAUI TGTACA 1 cut(s) 673
BstBAI YACGTR 1 cut(s) 239
BstBI TTCGAA 1 cut(s) 2099
BstC8I GCNNGC 8 cut(s) 351, 2277, 2466, 2835, 3075, 3392, 3808, 3897
BstDSI CCRYGG 2 cut(s) 1359, 3027
BstF5I GGATG 6 cut(s) 685, 1618, 1764, 1942, 2971, 3751
BstFNI CGCG 2 cut(s) 127, 3899
BstHHI GCGC 1 cut(s) 421
BstMAI GTCTC 6 cut(s) 264, 594, 947, 2142, 2934, 3036
BstMCI CGRYCG 1 cut(s) 3548
BstMWI GCNNNNNNNGC 7 cut(s) 16, 1046, 1907, 2188, 2465, 3202, 3807
BstNI CCWGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
BstNSI RCATGY 7 cut(s) 149, 2551, 3083, 3369, 3497, 3558, 3679
BstPAI GACNNNNGTC 1 cut(s) 692
BstSCI CCNGG 6 cut(s) 1507, 2459, 2669, 3353, 3379, 3963
BstSFI CTRYAG 1 cut(s) 1041
BstSLI GKGCMC 1 cut(s) 1811
BstUI CGCG 2 cut(s) 127, 3899
BstV2I GAAGAC 2 cut(s) 745, 2533
BstX2I RGATCY 3 cut(s) 316, 1406, 2481
BstYI RGATCY 3 cut(s) 316, 1406, 2481
BstZ17I GTATAC 1 cut(s) 3552
BsuI GTATCC 1 cut(s) 1704
BtgI CCRYGG 2 cut(s) 1359, 3027
BtrI CACGTC 2 cut(s) 416, 3003
BtsCI GGATG 6 cut(s) 685, 1618, 1764, 1942, 2971, 3751
BtsI GCAGTG 2 cut(s) 1645, 2139
BtsIMutI CAGTG 9 cut(s) 81, 696, 956, 1225, 1645, 2139, 2159, 2214, 2343
BtuMI TCGCGA 2 cut(s) 127, 3899
BveI ACCTGC 1 cut(s) 1328
Cac8I GCNNGC 8 cut(s) 351, 2277, 2466, 2835, 3075, 3392, 3808, 3897
CaiI CAGNNNCTG 2 cut(s) 19, 1049
CciI TCATGA 3 cut(s) 985, 1240, 1915
CfoI GCGC 1 cut(s) 421
Cfr10I RCCGGY 2 cut(s) 858, 1689
Cfr13I GGNCC 8 cut(s) 786, 882, 1092, 1427, 1479, 2590, 3404, 3456
CseI GACGC 1 cut(s) 42
CsiI ACCWGGT 1 cut(s) 1507
Csp6I GTAC 8 cut(s) 496, 577, 674, 707, 856, 1547, 2234, 3377
CspAI ACCGGT 1 cut(s) 1689
CspCI CAANNNNNGTGG 2 cut(s) 2233, 2268
CviQI GTAC 8 cut(s) 496, 577, 674, 707, 856, 1547, 2234, 3377
DraIII CACNNNGTG 1 cut(s) 1640
EaeI YGGCCR 4 cut(s) 37, 1251, 1492, 3351
Eam1104I CTCTTC 6 cut(s) 267, 1439, 1611, 2292, 3195, 3721
EarI CTCTTC 6 cut(s) 267, 1439, 1611, 2292, 3195, 3721
EciI GGCGGA 1 cut(s) 2710
Ecl136II GAGCTC 1 cut(s) 1449
Eco130I CCWWGG 4 cut(s) 382, 1482, 1932, 3027
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 2 cut(s) 1451, 1476
Eco31I GGTCTC 1 cut(s) 3036
Eco32I GATATC 2 cut(s) 1142, 3057
Eco47I GGWCC 4 cut(s) 882, 1427, 2590, 3404
Eco53kI GAGCTC 1 cut(s) 1449
Eco57I CTGAAG 3 cut(s) 2393, 3315, 3336
Eco88I CYCGRG 2 cut(s) 1736, 2196
EcoICRI GAGCTC 1 cut(s) 1449
EcoO109I RGGNCCY 2 cut(s) 882, 2590
EcoRII CCWGG 6 cut(s) 1507, 2459, 2669, 3353, 3379, 3963
EcoRV GATATC 2 cut(s) 1142, 3057
EcoT14I CCWWGG 4 cut(s) 382, 1482, 1932, 3027
EcoT22I ATGCAT 5 cut(s) 483, 1880, 2530, 2823, 3085
EcoT38I GRGCYC 2 cut(s) 1451, 1476
ErhI CCWWGG 4 cut(s) 382, 1482, 1932, 3027
Esp3I CGTCTC 1 cut(s) 2934
FalI AAGNNNNNCTT 6 cut(s) 2363, 2395, 2498, 2530, 3870, 3902
FaqI GGGAC 4 cut(s) 118, 1095, 1309, 2634
FauNDI CATATG 3 cut(s) 733, 769, 969
FbaI TGATCA 1 cut(s) 2917
FblI GTMKAC 1 cut(s) 3551
FokI GGATG 6 cut(s) 692, 1625, 1771, 1949, 2978, 3758
FriOI GRGCYC 2 cut(s) 1451, 1476
GlaI GCGC 1 cut(s) 420
GsaI CCCAGC 2 cut(s) 102, 786
GsuI CTGGAG 2 cut(s) 2692, 3716
HapII CCGG 2 cut(s) 859, 1690
HgaI GACGC 1 cut(s) 42
HhaI GCGC 1 cut(s) 421
Hin1I GRCGYC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 3 cut(s) 1669, 2604, 3363
HindII GTYRAC 3 cut(s) 1669, 2604, 3363
HindIII AAGCTT 3 cut(s) 2681, 3115, 3388
HpaI GTTAAC 2 cut(s) 2604, 3363
HpaII CCGG 2 cut(s) 859, 1690
Hpy166II GTNNAC 8 cut(s) 707, 1669, 1809, 2604, 2653, 2667, 3363, 3552
Hpy8I GTNNAC 8 cut(s) 707, 1669, 1809, 2604, 2653, 2667, 3363, 3552
Hpy99I CGWCG 4 cut(s) 10, 58, 417, 3490
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 1046, 1907, 2188, 2465, 3202, 3807
Hsp92I GRCGYC 1 cut(s) 53
HspAI GCGC 1 cut(s) 419
Ksp22I TGATCA 1 cut(s) 2917
KspAI GTTAAC 2 cut(s) 2604, 3363
LguI GCTCTTC 3 cut(s) 1439, 2292, 3195
LmnI GCTCC 4 cut(s) 1741, 2187, 2264, 2908
LweI GCATC 7 cut(s) 2029, 2429, 2557, 3107, 3186, 3289, 3736
MabI ACCWGGT 1 cut(s) 1507
MfeI CAATTG 2 cut(s) 23, 842
MflI RGATCY 3 cut(s) 316, 1406, 2481
MhlI GDGCHC 5 cut(s) 1451, 1476, 1811, 2269, 2839
MlsI TGGCCA 3 cut(s) 1253, 1494, 3353
MluNI TGGCCA 3 cut(s) 1253, 1494, 3353
MlyI GAGTC 3 cut(s) 2030, 2791, 2855
MmeI TCCRAC 3 cut(s) 1495, 1552, 1931
Mox20I TGGCCA 3 cut(s) 1253, 1494, 3353
Mph1103I ATGCAT 5 cut(s) 483, 1880, 2530, 2823, 3085
MroXI GAANNNNTTC 3 cut(s) 802, 1186, 3204
MscI TGGCCA 3 cut(s) 1253, 1494, 3353
MslI CAYNNNNRTG 6 cut(s) 165, 341, 597, 972, 1149, 2402
Msp20I TGGCCA 3 cut(s) 1253, 1494, 3353
MspA1I CMGCKG 3 cut(s) 16, 692, 1568
MspCI CTTAAG 1 cut(s) 511
MspI CCGG 2 cut(s) 859, 1690
MspR9I CCNGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
MunI CAATTG 2 cut(s) 23, 842
Mva1269I GAATGC 4 cut(s) 481, 1236, 2060, 3854
MvaI CCWGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
MvnI CGCG 2 cut(s) 127, 3899
MwoI GCNNNNNNNGC 7 cut(s) 16, 1046, 1907, 2188, 2465, 3202, 3807
NcoI CCATGG 1 cut(s) 3027
NdeI CATATG 3 cut(s) 733, 769, 969
NlaIV GGNNCC 5 cut(s) 508, 787, 884, 2341, 2483
NmeAIII GCCGAG 1 cut(s) 2869
NmuCI GTSAC 6 cut(s) 694, 1351, 1386, 1819, 2998, 3264
NruI TCGCGA 2 cut(s) 127, 3899
NsiI ATGCAT 5 cut(s) 483, 1880, 2530, 2823, 3085
NspI RCATGY 7 cut(s) 149, 2551, 3083, 3369, 3497, 3558, 3679
NspV TTCGAA 1 cut(s) 2099
PaeR7I CTCGAG 1 cut(s) 2196
PagI TCATGA 3 cut(s) 985, 1240, 1915
PaqCI CACCTGC 1 cut(s) 1328
PceI AGGCCT 1 cut(s) 203
PciI ACATGT 4 cut(s) 2547, 3365, 3493, 3554
PciSI GCTCTTC 3 cut(s) 1439, 2292, 3195
PctI GAATGC 4 cut(s) 481, 1236, 2060, 3854
PdmI GAANNNNTTC 3 cut(s) 802, 1186, 3204
PfeI GAWTC 8 cut(s) 929, 1009, 1400, 1976, 2081, 2894, 3474, 3751
PflMI CCANNNNNTGG 1 cut(s) 3964
PinAI ACCGGT 1 cut(s) 1689
PleI GAGTC 3 cut(s) 2029, 2790, 2854
PpsI GAGTC 3 cut(s) 2029, 2790, 2854
Ppu21I YACGTR 1 cut(s) 239
PpuMI RGGWCCY 2 cut(s) 882, 2590
PscI ACATGT 4 cut(s) 2547, 3365, 3493, 3554
PshAI GACNNNNGTC 1 cut(s) 692
Psp124BI GAGCTC 1 cut(s) 1451
Psp1406I AACGTT 2 cut(s) 1840, 2807
Psp5II RGGWCCY 2 cut(s) 882, 2590
Psp6I CCWGG 6 cut(s) 1507, 2459, 2669, 3353, 3379, 3963
PspFI CCCAGC 2 cut(s) 98, 782
PspGI CCWGG 6 cut(s) 1507, 2459, 2669, 3353, 3379, 3963
PspN4I GGNNCC 5 cut(s) 508, 787, 884, 2341, 2483
PspPI GGNCC 8 cut(s) 786, 882, 1092, 1427, 1479, 2590, 3404, 3456
PspPPI RGGWCCY 2 cut(s) 882, 2590
PspXI VCTCGAGB 1 cut(s) 2196
PstNI CAGNNNCTG 2 cut(s) 19, 1049
PsuI RGATCY 3 cut(s) 316, 1406, 2481
PvuII CAGCTG 3 cut(s) 16, 692, 1568
RruI TCGCGA 2 cut(s) 127, 3899
RsaI GTAC 8 cut(s) 497, 578, 675, 708, 857, 1548, 2235, 3378
RsaNI GTAC 8 cut(s) 496, 577, 674, 707, 856, 1547, 2234, 3377
RseI CAYNNNNRTG 6 cut(s) 165, 341, 597, 972, 1149, 2402
SacI GAGCTC 1 cut(s) 1451
SapI GCTCTTC 3 cut(s) 1439, 2292, 3195
Sau96I GGNCC 8 cut(s) 786, 882, 1092, 1427, 1479, 2590, 3404, 3456
ScaI AGTACT 1 cut(s) 2235
SchI GAGTC 3 cut(s) 2030, 2791, 2855
ScrFI CCNGG 6 cut(s) 1509, 2461, 2671, 3355, 3381, 3965
SduI GDGCHC 5 cut(s) 1451, 1476, 1811, 2269, 2839
SexAI ACCWGGT 1 cut(s) 1507
SfaNI GCATC 7 cut(s) 2029, 2429, 2557, 3107, 3186, 3289, 3736
SfcI CTRYAG 1 cut(s) 1041
Sfr274I CTCGAG 1 cut(s) 2196
SfuI TTCGAA 1 cut(s) 2099
SinI GGWCC 4 cut(s) 882, 1427, 2590, 3404
SlaI CTCGAG 1 cut(s) 2196
SmiMI CAYNNNNRTG 6 cut(s) 165, 341, 597, 972, 1149, 2402
SmlI CTYRAG 6 cut(s) 511, 1673, 2196, 2504, 2515, 3063
SmoI CTYRAG 6 cut(s) 511, 1673, 2196, 2504, 2515, 3063
SpeI ACTAGT 2 cut(s) 1148, 1903
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 6 cut(s) 40, 520, 721, 1610, 2695, 3174
SspI AATATT 5 cut(s) 451, 534, 571, 3325, 3667
SstI GAGCTC 1 cut(s) 1451
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 6 cut(s) 1507, 2459, 2669, 3353, 3379, 3963
StyI CCWWGG 4 cut(s) 382, 1482, 1932, 3027
TaqII GACCGA 1 cut(s) 3060
TatI WGTACW 4 cut(s) 495, 673, 706, 2233
TauI GCSGC 2 cut(s) 42, 3177
TfiI GAWTC 8 cut(s) 929, 1009, 1400, 1976, 2081, 2894, 3474, 3751
TscAI CASTG 9 cut(s) 88, 703, 963, 1225, 1645, 2139, 2159, 2214, 2350
TseFI GTSAC 6 cut(s) 694, 1351, 1386, 1819, 2998, 3264
Tsp45I GTSAC 6 cut(s) 694, 1351, 1386, 1819, 2998, 3264
TspGWI ACGGA 3 cut(s) 209, 386, 2474
TspRI CASTG 9 cut(s) 88, 703, 963, 1225, 1645, 2139, 2159, 2214, 2350
Van91I CCANNNNNTGG 1 cut(s) 3964
Vha464I CTTAAG 1 cut(s) 511
VneI GTGCAC 1 cut(s) 1807
VpaK11BI GGWCC 4 cut(s) 882, 1427, 2590, 3404
XbaI TCTAGA 3 cut(s) 2873, 3208, 3670
XceI RCATGY 7 cut(s) 149, 2551, 3083, 3369, 3497, 3558, 3679
XcmI CCANNNNNNNNNTGG 2 cut(s) 998, 2074
XhoI CTCGAG 1 cut(s) 2196
XmiI GTMKAC 1 cut(s) 3551
XmnI GAANNNNTTC 3 cut(s) 802, 1186, 3204
ZrmI AGTACT 1 cut(s) 2235
Zsp2I ATGCAT 5 cut(s) 483, 1880, 2530, 2823, 3085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.