AT5G02540

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010307
Physical Location & Seq
Forward (+)
568425 .. 570973
2549 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02540.1

Sequence Viewer

Length: 996 bp
ATGGGATTATATTCACTAATCACAGGAAGAAGAGGACCAAGTGGATTTGGTTCAGCTTCAACAGCTGAAGAGGTTACTCAAGGGATTGATGCAACTAATCTCACTGCAATTATCACAGGAGGGACAGGAGGAATAGGGATGGAGACAGCGAGAGTGCTGTCGAAGAGAGGTGCTCATGTGGTGATCGGTGCCCGAAACATGGGAGCTGCAGAAAACGCCAAAACAGAGATTCTCAGACAAAACGCAAACGCACGTGTCACTCTTCTTCAATTAGATCTCTCTTCCATCAAATCCATCAAAGCCTTTGTTCGTGAATTTCATGCCCTCCATCTTCCTCTCAATCTCCTCATAAACAATGCAGGAGTAATGTTCTGTCCATACCAACTCTCTGAAGATGGAATCGAATTGCAGTTTGCTACAAATCACATTGGTCACTTTCTGTTGACAAACTTGCTCTTGGACACAATGAAGAACACAGCTAAAACCAGTGGTGTTGAAGGAAGAATTTTGAATGTATCATCTGTAGCTCATATCTATACTTACCAAGAAGGAATCCAATTCGACAGCATCAATGACATTTGCAGTTACTCGGATAAGCGAGCTTACGGACAATCGAAACTAGCCAATATATTGCATGCTAATGAGCTCTCCCGTCAGCTTCAGGAAGAAGGAGTAAACATAACAGCAAATTCAGTTCACCCTGGTCTTATTCTCACCAATCTCTTCCAACACACTGCTCTTTTAATGAGGTTCCTGAAGTTCTTCAGCTTCTACTTGTGGAAAAATATACCTCAGGGAGCAGCAACAACATGTTATGTTGCTCTGCATCCAAGTGTGAAGGGAGTAACAGGAAAGTACTTTGCTGATTGCAACGAAGTCACTCCAAGCAAACTTGCTAGAGATGAGACTTTGGCACAGAAACTCTGGGATTTCAGTGTTAAGCTCATTAACTCGGTTTCTAAAAAGAACTACCTCGGTTTCGATGACACCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
adh_short

Protein Analysis

331

Amino Acids

36.19

Weight (kDa)

8.57

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 35 - 244 3.7e-30 short chain dehydrogenase
KR PF08659 35 - 123 1.4e-10 KR domain
Epimerase PF01370 37 - 219 3.2e-07 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 43 - 247 2.9e-15 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AcsI RAATTY 3 cut(s) 314, 504, 688
AcuI CTGAAG 5 cut(s) 87, 411, 644, 748, 776
AcvI CACGTG 1 cut(s) 254
AfaI GTAC 1 cut(s) 857
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 2 cut(s) 253, 809
AgsI TTSAA 4 cut(s) 60, 269, 497, 511
AjnI CCWGG 1 cut(s) 700
Alw21I GWGCWC 2 cut(s) 175, 648
Alw26I GTCTC 2 cut(s) 137, 899
ApeKI GCWGC 2 cut(s) 206, 800
ApoI RAATTY 3 cut(s) 314, 504, 688
Asp700I GAANNNNTTC 1 cut(s) 761
AspS9I GGNCC 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 193, 689, 706
AvaII GGWCC 1 cut(s) 35
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 648
BbrPI CACGTG 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 175, 648
BbvI GCAGC 2 cut(s) 193, 812
BccI CCATC 5 cut(s) 133, 293, 302, 336, 389
BciT130I CCWGG 1 cut(s) 702
BcoDI GTCTC 2 cut(s) 137, 899
BfaI CTAG 2 cut(s) 620, 897
BfmI CTRYAG 2 cut(s) 207, 522
BglII AGATCT 1 cut(s) 274
BisI GCNGC 2 cut(s) 207, 801
BlsI GCNGC 2 cut(s) 208, 802
BmcAI AGTACT 1 cut(s) 857
Bme1390I CCNGG 1 cut(s) 702
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 2 cut(s) 190, 752
BmrFI CCNGG 1 cut(s) 702
BmsI GCATC 3 cut(s) 79, 576, 835
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpuEI CTTGAG 1 cut(s) 63
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 700, 973
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 486
Bse21I CCTNAGG 1 cut(s) 792
BseBI CCWGG 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 700, 973
BseGI GGATG 2 cut(s) 144, 826
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 247, 806
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 1 cut(s) 335
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 2 cut(s) 193, 812
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 175, 648
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 137, 899
BsmFI GGGAC 1 cut(s) 136
Bsp1286I GDGCHC 3 cut(s) 175, 193, 648
Bsp143I GATC 2 cut(s) 183, 274
BspCNI CTCAG 2 cut(s) 246, 805
BspLI GGNNCC 2 cut(s) 190, 752
BspMAI CTGCAG 1 cut(s) 211
BspT107I GGYRCC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 486
BssECI CCNNGG 2 cut(s) 700, 973
BssMI GATC 2 cut(s) 183, 274
Bst2UI CCWGG 1 cut(s) 702
Bst6I CTCTTC 6 cut(s) 25, 63, 158, 267, 286, 728
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 600, 636
BstDEI CTNAG 2 cut(s) 233, 792
BstF5I GGATG 2 cut(s) 144, 826
BstKTI GATC 2 cut(s) 186, 277
BstMAI GTCTC 2 cut(s) 137, 899
BstMBI GATC 2 cut(s) 183, 274
BstMWI GCNNNNNNNGC 2 cut(s) 62, 215
BstNI CCWGG 1 cut(s) 702
BstNSI RCATGY 2 cut(s) 638, 813
BstSCI CCNGG 1 cut(s) 700
BstSFI CTRYAG 2 cut(s) 207, 522
BstSLI GKGCMC 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 193, 812
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
Bsu36I CCTNAGG 1 cut(s) 792
BtsCI GGATG 2 cut(s) 144, 826
BtsI GCAGTG 2 cut(s) 102, 732
BtsIMutI CAGTG 4 cut(s) 102, 493, 732, 940
Cac8I GCNNGC 2 cut(s) 600, 636
Cfr13I GGNCC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 856
CviAII CATG 5 cut(s) 176, 199, 320, 635, 810
CviQI GTAC 1 cut(s) 856
DdeI CTNAG 2 cut(s) 233, 792
DpnI GATC 2 cut(s) 185, 276
DpnII GATC 2 cut(s) 183, 274
Eam1104I CTCTTC 6 cut(s) 25, 63, 158, 267, 286, 728
EarI CTCTTC 6 cut(s) 25, 63, 158, 267, 286, 728
Ecl136II GAGCTC 1 cut(s) 646
Eco24I GRGCYC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 646
Eco57I CTGAAG 5 cut(s) 87, 411, 644, 748, 776
Eco72I CACGTG 1 cut(s) 254
Eco81I CCTNAGG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 646
EcoRII CCWGG 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 648
FaeI CATG 5 cut(s) 179, 202, 323, 638, 813
FaqI GGGAC 1 cut(s) 136
FatI CATG 5 cut(s) 175, 198, 319, 634, 809
Fnu4HI GCNGC 2 cut(s) 207, 801
FokI GGATG 2 cut(s) 151, 813
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 2 cut(s) 207, 801
FspBI CTAG 2 cut(s) 620, 897
GluI GCNGC 2 cut(s) 207, 801
Hin1II CATG 5 cut(s) 179, 202, 323, 638, 813
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 3 cut(s) 229, 399, 552
HphI GGTGA 3 cut(s) 193, 689, 706
Hpy166II GTNNAC 3 cut(s) 444, 676, 697
Hpy188I TCNGA 3 cut(s) 236, 391, 592
Hpy188III TCNNGA 3 cut(s) 311, 662, 754
Hpy8I GTNNAC 3 cut(s) 444, 676, 697
HpyAV CCTTC 4 cut(s) 491, 542, 662, 832
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 9 cut(s) 92, 107, 209, 359, 409, 582, 634, 826, 870
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 215
HpyF3I CTNAG 2 cut(s) 233, 792
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 5 cut(s) 179, 202, 323, 638, 813
Kzo9I GATC 2 cut(s) 183, 274
LmnI GCTCC 2 cut(s) 203, 797
Lsp1109I GCAGC 2 cut(s) 193, 812
LweI GCATC 3 cut(s) 79, 576, 835
MaeI CTAG 2 cut(s) 620, 897
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 6 cut(s) 73, 256, 431, 584, 844, 877
MalI GATC 2 cut(s) 185, 276
MboI GATC 2 cut(s) 183, 274
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 3 cut(s) 175, 193, 648
MluCI AATT 7 cut(s) 108, 269, 314, 404, 504, 557, 688
MmeI TCCRAC 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 761
MseI TTAA 3 cut(s) 743, 939, 948
MslI CAYNNNNRTG 2 cut(s) 639, 831
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 702
MvaI CCWGG 1 cut(s) 702
MwoI GCNNNNNNNGC 2 cut(s) 62, 215
NdeII GATC 2 cut(s) 183, 274
NlaIII CATG 5 cut(s) 179, 202, 323, 638, 813
NlaIV GGNNCC 2 cut(s) 190, 752
NmuCI GTSAC 3 cut(s) 256, 431, 877
NspI RCATGY 2 cut(s) 638, 813
PaeI GCATGC 1 cut(s) 638
PciI ACATGT 1 cut(s) 809
PdmI GAANNNNTTC 1 cut(s) 761
PfeI GAWTC 3 cut(s) 229, 399, 552
PkrI GCNGC 2 cut(s) 208, 802
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 254
PscI ACATGT 1 cut(s) 809
Psp124BI GAGCTC 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 700
PspCI CACGTG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 700
PspN4I GGNNCC 2 cut(s) 190, 752
PspPI GGNCC 1 cut(s) 35
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 857
RsaNI GTAC 1 cut(s) 856
RseI CAYNNNNRTG 2 cut(s) 639, 831
SacI GAGCTC 1 cut(s) 648
SaqAI TTAA 3 cut(s) 743, 939, 948
SatI GCNGC 2 cut(s) 207, 801
Sau3AI GATC 2 cut(s) 183, 274
Sau96I GGNCC 1 cut(s) 35
ScaI AGTACT 1 cut(s) 857
ScrFI CCNGG 1 cut(s) 702
SduI GDGCHC 3 cut(s) 175, 193, 648
SfaNI GCATC 3 cut(s) 79, 576, 835
SfcI CTRYAG 2 cut(s) 207, 522
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 2 cut(s) 639, 831
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SphI GCATGC 1 cut(s) 638
Sse9I AATT 7 cut(s) 108, 269, 314, 404, 504, 557, 688
SspMI CTAG 2 cut(s) 620, 897
SstI GAGCTC 1 cut(s) 648
StyD4I CCNGG 1 cut(s) 700
TaiI ACGT 1 cut(s) 256
TaqI TCGA 5 cut(s) 161, 402, 561, 614, 981
TasI AATT 7 cut(s) 108, 269, 314, 404, 504, 557, 688
TatI WGTACW 1 cut(s) 855
TfiI GAWTC 3 cut(s) 229, 399, 552
Tru1I TTAA 3 cut(s) 743, 939, 948
Tru9I TTAA 3 cut(s) 743, 939, 948
TscAI CASTG 4 cut(s) 109, 493, 739, 940
TseFI GTSAC 3 cut(s) 256, 431, 877
TseI GCWGC 2 cut(s) 206, 800
Tsp45I GTSAC 3 cut(s) 256, 431, 877
TspDTI ATGAA 2 cut(s) 308, 482
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 4 cut(s) 109, 493, 739, 940
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 3 cut(s) 314, 504, 688
XceI RCATGY 2 cut(s) 638, 813
XmnI GAANNNNTTC 1 cut(s) 761
XspI CTAG 2 cut(s) 620, 897
ZrmI AGTACT 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.