Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010307

Physical Location & Seq

Genomic Coordinates Reverse (-)

21031927 .. 21037649

Gene Structure 5723 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1124100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGGCATCTTTTCGTTGGTTACAGGAAGGCCAGGGCCTAGTGGATTTGGATCAGCTTCCACTGCTGAGCAGGTTACTGAAGGAGTTGATGCAACCAACCTGACTGCCATTGTGACTGGAGGGGCAAGTGGGATTGGATTTGAGGCTGCAAGGGTGTTGGCACTTCGCAGAGCCCATGTCATCATTGCTGCTAGAAACCTTGAGGCTGCAAACGAAGCTAAACAACTTATTCTCAAAGAAAATAAAACTGCTCGAGTGGATGTTCTCAAACTTGACCTCTGCTCTATCAAGTCTGTTAGGGCATTCGTCGATAGCTTCAGCGCTCTTGATCTCCCGCTCAACCTCTTAATAAACAATGCTGGTGTTATGTTCTGCCCACACCAGCTTTCGGAAGATGGAATTGAGATGCAATTTGCAACCAACCATCTTGGTCATTTTCTCTTAACAAACCTTCTCCTTGAGAAAATGAAGCATACAGCTAGAACTACTGGTGTAGAAGGAAGGATTGTGAACTTGTCATCAATTGCTCGTATACACTCTTATGAAGGTGGGATTCGATTCAAAGAGATCAATGATCGAAGCAGTTACTCAGACAAAAGGGCATATGGGCAATCCAAATTAGCAAACATACTTCATGCCAATGAGCTCTCTCGTCGATTGCAGGGAGAAGGTGTGAACATAACTGTCAACTCGGTCCACCCAGGAATCATAATGACACCCCTCATGAGGCATTCTACACTTATGATGAAAACGTTAAGGTTGTTCACCTTTTGGATGTGGAAGAATGTACATCAGGGAGCAGCCACAACATTGTATGTTGCTCTGCATCCAAAACTGAAAGGTGTGACCGGAAAATACTACGTCGACTGCAACGAGATGAAACCCAGCTCATATGCTAGAGATGAAAAGTTGGCCAATAAACTTTGGGACTTCAGCAACAAACTGATTGATGGAGCTTCCGTTCCAAAAGCTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00830 ko01100 map00830 map01100

Pfam Domains

Protein Families

adh_short

Protein Analysis

326

Amino Acids

35.66

Weight (kDa)

9.43

Isoelectric Point (pI)

27.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	35 - 181	3.7e-23	short chain dehydrogenase
KR	PF08659	35 - 123	5.5e-07	KR domain
adh_short_C2	PF13561	42 - 246	3.8e-15	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37540 AT2G37540 AT5G02540
fragaria_vesca	FvH4_6g14170 FvH4_6g14170
malus_domestica	MD04G1123600.v1.1 MD04G1124100.v1.1 MD12G1139500.v1.1
prunus_persica	Prupe.6G251300_v2.0.a1 Prupe.6G251300_v2.0.a1 Prupe.6G251400_v2.0.a1
pyrus_communis	pycom12g13380
rosa_chinensis	RchiOBHm_Chr3g0467121
rosa_laevigata	RLG00000024524
rosa_multiflora	Rmu_sc0018989.1_g000002
rosa_roxburghii	Rroxscaffold_6G00413870
rosa_rugosa	Rorug03G0085500
rosa_samantha	Rh3BG154900 Rh3CG154700 Rh3DG155800
rosa_wichuraiana	Rw3G012640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	61
AccBSI	CCGCTC	1 cut(s)	337
AccI	GTMKAC	2 cut(s)	532, 864
AciI	CCGC	1 cut(s)	335
AclI	AACGTT	1 cut(s)	752
AclWI	GGATC	1 cut(s)	58
AcoI	YGGCCR	1 cut(s)	912
AcuI	CTGAAG	3 cut(s)	99, 301, 916
AfaI	GTAC	1 cut(s)	789
AfeI	AGCGCT	1 cut(s)	322
AfiI	CCNNNNNNNGG	2 cut(s)	388, 726
AgsI	TTSAA	1 cut(s)	562
AjnI	CCWGG	2 cut(s)	31, 700
AloI	GAACNNNNNNTCC	2 cut(s)	945, 977
AluBI	AGCT	9 cut(s)	56, 218, 315, 385, 479, 646, 888, 956, 971
AluI	AGCT	9 cut(s)	56, 218, 315, 385, 479, 646, 888, 956, 971
Alw21I	GWGCWC	1 cut(s)	648
AlwI	GGATC	1 cut(s)	58
Ama87I	CYCGRG	1 cut(s)	252
Aor51HI	AGCGCT	1 cut(s)	322
AoxI	GGCC	3 cut(s)	29, 35, 912
ApeKI	GCWGC	4 cut(s)	146, 188, 206, 800
AspLEI	GCGC	1 cut(s)	323
AspS9I	GGNCC	2 cut(s)	35, 694
AsuHPI	GGTGA	1 cut(s)	757
AvaI	CYCGRG	1 cut(s)	252
AvaII	GGWCC	1 cut(s)	694
BalI	TGGCCA	1 cut(s)	914
BanII	GRGCYC	2 cut(s)	175, 648
Bbv12I	GWGCWC	1 cut(s)	648
BbvI	GCAGC	4 cut(s)	133, 175, 193, 812
BccI	CCATC	3 cut(s)	389, 432, 944
BciT130I	CCWGG	2 cut(s)	33, 702
BfaI	CTAG	4 cut(s)	39, 192, 480, 897
BfoI	RGCGCY	1 cut(s)	324
BfuAI	ACCTGC	1 cut(s)	61
BisI	GCNGC	4 cut(s)	147, 189, 207, 801
BlpI	GCTNAGC	1 cut(s)	66
BlsI	GCNGC	4 cut(s)	148, 190, 208, 802
Bme1390I	CCNGG	2 cut(s)	33, 702
Bme18I	GGWCC	1 cut(s)	694
BmeT110I	CYCGRG	1 cut(s)	252
BmgT120I	GGNCC	2 cut(s)	35, 694
BmrFI	CCNGG	2 cut(s)	33, 702
BmsI	GCATC	4 cut(s)	15, 79, 396, 835
BpmI	CTGGAG	1 cut(s)	138
Bpu1102I	GCTNAGC	1 cut(s)	66
BpuEI	CTTGAG	2 cut(s)	221, 479
BsaBI	GATNNNNATC	1 cut(s)	49
BsaJI	CCNNGG	2 cut(s)	32, 700
BsaWI	WCCGGW	1 cut(s)	848
BsaXI	ACNNNNNCTCC	4 cut(s)	657, 687, 945, 975
Bsc4I	CCNNNNNNNGG	2 cut(s)	388, 726
Bse1I	ACTGG	2 cut(s)	121, 493
Bse3DI	GCAATG	1 cut(s)	183
Bse8I	GATNNNNATC	1 cut(s)	49
BseBI	CCWGG	2 cut(s)	33, 702
BseDI	CCNNGG	2 cut(s)	32, 700
BseGI	GGATG	3 cut(s)	265, 780, 826
BseJI	GATNNNNATC	1 cut(s)	49
BseLI	CCNNNNNNNGG	2 cut(s)	388, 726
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	2 cut(s)	57, 603
BseNI	ACTGG	2 cut(s)	121, 493
BseXI	GCAGC	4 cut(s)	133, 175, 193, 812
BseYI	CCCAGC	1 cut(s)	884
BshFI	GGCC	3 cut(s)	31, 37, 914
BsiHKAI	GWGCWC	1 cut(s)	648
BsiHKCI	CYCGRG	1 cut(s)	252
BsiSI	CCGG	1 cut(s)	849
BslFI	GGGAC	1 cut(s)	941
BslI	CCNNNNNNNGG	2 cut(s)	388, 726
BsmFI	GGGAC	1 cut(s)	941
BsmI	GAATGC	2 cut(s)	302, 730
BsnI	GGCC	3 cut(s)	31, 37, 914
BsoBI	CYCGRG	1 cut(s)	252
Bsp1286I	GDGCHC	2 cut(s)	175, 648
Bsp1407I	TGTACA	1 cut(s)	787
Bsp143I	GATC	4 cut(s)	50, 328, 567, 574
Bsp1720I	GCTNAGC	1 cut(s)	66
BspACI	CCGC	1 cut(s)	335
BspANI	GGCC	3 cut(s)	31, 37, 914
BspCNI	CTCAG	2 cut(s)	58, 602
BspHI	TCATGA	1 cut(s)	723
BspMI	ACCTGC	1 cut(s)	61
BspPI	GGATC	1 cut(s)	58
BsrBI	CCGCTC	1 cut(s)	337
BsrDI	GCAATG	1 cut(s)	183
BsrGI	TGTACA	1 cut(s)	787
BsrI	ACTGG	2 cut(s)	121, 493
BssECI	CCNNGG	2 cut(s)	32, 700
BssMI	GATC	4 cut(s)	50, 328, 567, 574
BssNAI	GTATAC	1 cut(s)	533
Bst1107I	GTATAC	1 cut(s)	533
Bst2UI	CCWGG	2 cut(s)	33, 702
Bst4CI	ACNGT	1 cut(s)	685
BstAUI	TGTACA	1 cut(s)	787
BstDEI	CTNAG	2 cut(s)	66, 589
BstF5I	GGATG	3 cut(s)	265, 780, 826
BstH2I	RGCGCY	1 cut(s)	324
BstHHI	GCGC	1 cut(s)	323
BstKTI	GATC	4 cut(s)	53, 331, 570, 577
BstMBI	GATC	4 cut(s)	50, 328, 567, 574
BstMWI	GCNNNNNNNGC	2 cut(s)	62, 215
BstNI	CCWGG	2 cut(s)	33, 702
BstSCI	CCNGG	2 cut(s)	31, 700
BstV1I	GCAGC	4 cut(s)	133, 175, 193, 812
BstZ17I	GTATAC	1 cut(s)	533
BsuRI	GGCC	3 cut(s)	31, 37, 914
BtsCI	GGATG	3 cut(s)	265, 780, 826
BtsI	GCAGTG	1 cut(s)	60
BtsIMutI	CAGTG	1 cut(s)	60
BveI	ACCTGC	1 cut(s)	61
CciI	TCATGA	1 cut(s)	723
CfoI	GCGC	1 cut(s)	323
Cfr13I	GGNCC	2 cut(s)	35, 694
Csp6I	GTAC	1 cut(s)	788
CviAII	CATG	3 cut(s)	176, 635, 724
CviQI	GTAC	1 cut(s)	788
DdeI	CTNAG	2 cut(s)	66, 589
DpnI	GATC	4 cut(s)	52, 330, 569, 576
DpnII	GATC	4 cut(s)	50, 328, 567, 574
EaeI	YGGCCR	1 cut(s)	912
Ecl136II	GAGCTC	1 cut(s)	646
Eco24I	GRGCYC	2 cut(s)	175, 648
Eco47I	GGWCC	1 cut(s)	694
Eco47III	AGCGCT	1 cut(s)	322
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	3 cut(s)	99, 301, 916
Eco88I	CYCGRG	1 cut(s)	252
EcoICRI	GAGCTC	1 cut(s)	646
EcoO109I	RGGNCCY	1 cut(s)	35
EcoRII	CCWGG	2 cut(s)	31, 700
EcoT38I	GRGCYC	2 cut(s)	175, 648
FaeI	CATG	3 cut(s)	179, 638, 727
FaqI	GGGAC	1 cut(s)	941
FatI	CATG	3 cut(s)	175, 634, 723
FauI	CCCGC	1 cut(s)	342
FauNDI	CATATG	2 cut(s)	604, 892
FblI	GTMKAC	2 cut(s)	532, 864
Fnu4HI	GCNGC	4 cut(s)	147, 189, 207, 801
FokI	GGATG	3 cut(s)	272, 787, 813
FriOI	GRGCYC	2 cut(s)	175, 648
Fsp4HI	GCNGC	4 cut(s)	147, 189, 207, 801
FspBI	CTAG	4 cut(s)	39, 192, 480, 897
GlaI	GCGC	1 cut(s)	322
GluI	GCNGC	4 cut(s)	147, 189, 207, 801
GsaI	CCCAGC	1 cut(s)	888
GsuI	CTGGAG	1 cut(s)	138
HaeII	RGCGCY	1 cut(s)	324
HaeIII	GGCC	3 cut(s)	31, 37, 914
HapII	CCGG	1 cut(s)	849
HhaI	GCGC	1 cut(s)	323
Hin1II	CATG	3 cut(s)	179, 638, 727
Hin6I	GCGC	1 cut(s)	321
HinP1I	GCGC	1 cut(s)	321
HincII	GTYRAC	2 cut(s)	688, 865
HindII	GTYRAC	2 cut(s)	688, 865
HinfI	GANTC	3 cut(s)	553, 558, 705
HpaII	CCGG	1 cut(s)	849
HphI	GGTGA	1 cut(s)	757
Hpy166II	GTNNAC	7 cut(s)	511, 533, 676, 688, 697, 765, 865
Hpy188I	TCNGA	2 cut(s)	391, 592
Hpy188III	TCNNGA	2 cut(s)	326, 724
Hpy8I	GTNNAC	7 cut(s)	511, 533, 676, 688, 697, 765, 865
Hpy99I	CGWCG	3 cut(s)	311, 657, 866
HpyAV	CCTTC	7 cut(s)	21, 74, 461, 491, 495, 539, 662
HpyCH4III	ACNGT	1 cut(s)	685
HpyCH4IV	ACGT	2 cut(s)	752, 861
HpyCH4V	TGCA	8 cut(s)	92, 149, 209, 409, 416, 661, 826, 870
HpyF10VI	GCNNNNNNNGC	2 cut(s)	62, 215
HpyF3I	CTNAG	2 cut(s)	66, 589
HpySE526I	ACGT	2 cut(s)	752, 861
Hsp92II	CATG	3 cut(s)	179, 638, 727
HspAI	GCGC	1 cut(s)	321
Kzo9I	GATC	4 cut(s)	50, 328, 567, 574
LmnI	GCTCC	2 cut(s)	797, 953
Lsp1109I	GCAGC	4 cut(s)	133, 175, 193, 812
LweI	GCATC	4 cut(s)	15, 79, 396, 835
MaeI	CTAG	4 cut(s)	39, 192, 480, 897
MaeII	ACGT	2 cut(s)	752, 861
MaeIII	GTNAC	5 cut(s)	19, 73, 112, 584, 844
MalI	GATC	4 cut(s)	52, 330, 569, 576
MbiI	CCGCTC	1 cut(s)	337
MboI	GATC	4 cut(s)	50, 328, 567, 574
MboII	GAAGA	2 cut(s)	404, 793
MfeI	CAATTG	1 cut(s)	522
MhlI	GDGCHC	2 cut(s)	175, 648
MlsI	TGGCCA	1 cut(s)	914
MluCI	AATT	4 cut(s)	399, 410, 522, 617
MluNI	TGGCCA	1 cut(s)	914
MnlI	CCTC	7 cut(s)	113, 136, 196, 287, 353, 720, 731
Mox20I	TGGCCA	1 cut(s)	914
MscI	TGGCCA	1 cut(s)	914
MseI	TTAA	3 cut(s)	347, 443, 755
MslI	CAYNNNNRTG	2 cut(s)	540, 639
Msp20I	TGGCCA	1 cut(s)	914
MspI	CCGG	1 cut(s)	849
MspR9I	CCNGG	2 cut(s)	33, 702
MunI	CAATTG	1 cut(s)	522
Mva1269I	GAATGC	2 cut(s)	302, 730
MvaI	CCWGG	2 cut(s)	33, 702
MwoI	GCNNNNNNNGC	2 cut(s)	62, 215
NdeI	CATATG	2 cut(s)	604, 892
NdeII	GATC	4 cut(s)	50, 328, 567, 574
NlaIII	CATG	3 cut(s)	179, 638, 727
NmuCI	GTSAC	2 cut(s)	112, 844
PaeR7I	CTCGAG	1 cut(s)	252
PagI	TCATGA	1 cut(s)	723
PcsI	WCGNNNNNNNCGW	1 cut(s)	870
PctI	GAATGC	2 cut(s)	302, 730
PfeI	GAWTC	3 cut(s)	553, 558, 705
PkrI	GCNGC	4 cut(s)	148, 190, 208, 802
Psp124BI	GAGCTC	1 cut(s)	648
Psp1406I	AACGTT	1 cut(s)	752
Psp6I	CCWGG	2 cut(s)	31, 700
PspFI	CCCAGC	1 cut(s)	884
PspGI	CCWGG	2 cut(s)	31, 700
PspPI	GGNCC	2 cut(s)	35, 694
PspXI	VCTCGAGB	1 cut(s)	252
RsaI	GTAC	1 cut(s)	789
RsaNI	GTAC	1 cut(s)	788
RseI	CAYNNNNRTG	2 cut(s)	540, 639
SacI	GAGCTC	1 cut(s)	648
SalI	GTCGAC	1 cut(s)	863
SaqAI	TTAA	3 cut(s)	347, 443, 755
SatI	GCNGC	4 cut(s)	147, 189, 207, 801
Sau3AI	GATC	4 cut(s)	50, 328, 567, 574
Sau96I	GGNCC	2 cut(s)	35, 694
ScrFI	CCNGG	2 cut(s)	33, 702
SduI	GDGCHC	2 cut(s)	175, 648
SfaNI	GCATC	4 cut(s)	15, 79, 396, 835
Sfr274I	CTCGAG	1 cut(s)	252
SinI	GGWCC	1 cut(s)	694
SlaI	CTCGAG	1 cut(s)	252
SmiMI	CAYNNNNRTG	2 cut(s)	540, 639
SmlI	CTYRAG	3 cut(s)	200, 252, 458
SmoI	CTYRAG	3 cut(s)	200, 252, 458
Sse9I	AATT	4 cut(s)	399, 410, 522, 617
SsiI	CCGC	1 cut(s)	335
SspMI	CTAG	4 cut(s)	39, 192, 480, 897
SstI	GAGCTC	1 cut(s)	648
StyD4I	CCNGG	2 cut(s)	31, 700
TaaI	ACNGT	1 cut(s)	685
TaiI	ACGT	2 cut(s)	755, 864
TaqI	TCGA	6 cut(s)	253, 309, 556, 577, 655, 864
TaqII	GACCGA	1 cut(s)	682
TasI	AATT	4 cut(s)	399, 410, 522, 617
TatI	WGTACW	1 cut(s)	787
TfiI	GAWTC	3 cut(s)	553, 558, 705
Tru1I	TTAA	3 cut(s)	347, 443, 755
Tru9I	TTAA	3 cut(s)	347, 443, 755
TscAI	CASTG	1 cut(s)	67
TseFI	GTSAC	2 cut(s)	112, 844
TseI	GCWGC	4 cut(s)	146, 188, 206, 800
Tsp45I	GTSAC	2 cut(s)	112, 844
TspDTI	ATGAA	6 cut(s)	482, 558, 623, 761, 893, 918
TspGWI	ACGGA	1 cut(s)	949
TspRI	CASTG	1 cut(s)	67
VpaK11BI	GGWCC	1 cut(s)	694
XhoI	CTCGAG	1 cut(s)	252
XmiI	GTMKAC	2 cut(s)	532, 864
XspI	CTAG	4 cut(s)	39, 192, 480, 897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.