Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010307

Physical Location & Seq

Genomic Coordinates Reverse (-)

20914814 .. 20919358

Gene Structure 4545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1123600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 969 bp

ATGGGTTTCTTTGCATTGGTGACGGGAAGGCGAGGGCCAAGTGGGTTTGGATCAGCTTCCACTGCTGAGCAGGTTACTGAAGGAGTTGATGCATCCAACCTCACCGCCATTGTTACTGGAGGGGCTAGTGGGATTGGGTTGGAGACTGCAAGGGTGTTGGCACTCCGCGGAGCGCATGTCATCATCGCCGCCAGAAACCTTGAGGCTGCAAACGAAGCTAAACAGCTTATTCTTAAAGACAATCAAACTGCTCGAATCGATATTCTGAAACTTGACCTCTCCTCTATCAAGTCTGTTAGAGCATTCGTCGATAGTTTCAACGCTCTCGATCTCCCTCTCAACCTCTTAATAAACAATGCTGGTGTTATGTTCTGCCCCTTCCAGCTTTCAGAAGATGGAATAGAGATGCAATTTGCAACCAATCATCTTGGTCATTTTCTGTTAACAAACCTTCTGCTTGAAAAAATGAAGACTACAGTTCAAACTACTGGTGTGGAAGCCAGAATTGTGAATTTGTCATCGATTGCTCATACAAGCACTTACGAAGGCGGGATTCGATTGGACAGGATCAATGATCCAGATAGTTACTCCACGTTAAATGCATACGGGCAGTCCAAATTAGCAAACATACTGCACGCCAATGAGCTCTCTCGTCGATTGCAGGAAGAAGGGGTGAGCATTACAGTCAACTCTGTCCACCCAGGATTGATCACGACACGTCTCATGAGGCATAGTCCTCTTTTGATGAAGGCTTTTAAGATATTCACCTTTTGCATGTGGAAGAACGTACCTCAGGGAGCAGCCACAACATTGTACGTGGCACTGCACCCAAAGTTGAAAGGTATAAGCGGAAAATACTATGTCGACTGCAATGAGACGAAACCCAGCAAAGATGCTGGTGATGAAAACTTGGCTAAAAAGCTTTGTGATTTCAGCAACAAGTTGATTGATGGAGCTTCCAAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00830 ko01100 map00830 map01100

Pfam Domains

Protein Families

adh_short

Protein Analysis

323

Amino Acids

34.87

Weight (kDa)

8.59

Isoelectric Point (pI)

26.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	35 - 182	1.4e-24	short chain dehydrogenase
KR	PF08659	35 - 123	4.6e-09	KR domain
adh_short_C2	PF13561	42 - 246	6e-16	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010307)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37540 AT2G37540 AT5G02540
fragaria_vesca	FvH4_6g14170 FvH4_6g14170
malus_domestica	MD04G1123600.v1.1 MD04G1124100.v1.1 MD12G1139500.v1.1
prunus_persica	Prupe.6G251300_v2.0.a1 Prupe.6G251300_v2.0.a1 Prupe.6G251400_v2.0.a1
pyrus_communis	pycom12g13380
rosa_chinensis	RchiOBHm_Chr3g0467121
rosa_laevigata	RLG00000024524
rosa_multiflora	Rmu_sc0018989.1_g000002
rosa_roxburghii	Rroxscaffold_6G00413870
rosa_rugosa	Rorug03G0085500
rosa_samantha	Rh3BG154900 Rh3CG154700 Rh3DG155800
rosa_wichuraiana	Rw3G012640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	61
AccI	GTMKAC	1 cut(s)	864
AccII	CGCG	1 cut(s)	168
AciI	CCGC	6 cut(s)	105, 166, 168, 189, 549, 849
AclWI	GGATC	3 cut(s)	58, 569, 575
AcsI	RAATTY	1 cut(s)	511
AcuI	CTGAAG	1 cut(s)	99
AfaI	GTAC	2 cut(s)	789, 815
AflIII	ACRYGT	1 cut(s)	716
AgsI	TTSAA	4 cut(s)	319, 461, 482, 838
AjiI	CACGTC	1 cut(s)	719
AjnI	CCWGG	1 cut(s)	700
AluBI	AGCT	7 cut(s)	56, 218, 226, 385, 646, 922, 956
AluI	AGCT	7 cut(s)	56, 218, 226, 385, 646, 922, 956
Alw21I	GWGCWC	1 cut(s)	648
Alw26I	GTCTC	3 cut(s)	137, 725, 869
AlwI	GGATC	3 cut(s)	58, 569, 575
AoxI	GGCC	1 cut(s)	35
ApeKI	GCWGC	2 cut(s)	206, 800
ApoI	RAATTY	1 cut(s)	511
AspLEI	GCGC	1 cut(s)	175
AspS9I	GGNCC	1 cut(s)	35
AsuHPI	GGTGA	5 cut(s)	31, 94, 685, 757, 911
AxyI	CCTNAGG	1 cut(s)	792
BanII	GRGCYC	1 cut(s)	648
BbsI	GAAGAC	1 cut(s)	476
Bbv12I	GWGCWC	1 cut(s)	648
BbvI	GCAGC	2 cut(s)	193, 812
BccI	CCATC	2 cut(s)	389, 944
BciT130I	CCWGG	1 cut(s)	702
BclI	TGATCA	1 cut(s)	708
BcoDI	GTCTC	3 cut(s)	137, 725, 869
BfaI	CTAG	1 cut(s)	126
BfmI	CTRYAG	1 cut(s)	474
BfuAI	ACCTGC	1 cut(s)	61
BisI	GCNGC	3 cut(s)	189, 207, 801
BlpI	GCTNAGC	1 cut(s)	66
BlsI	GCNGC	3 cut(s)	190, 208, 802
Bme1390I	CCNGG	1 cut(s)	702
BmgBI	CACGTC	1 cut(s)	719
BmgT120I	GGNCC	1 cut(s)	35
BmrFI	CCNGG	1 cut(s)	702
BmsI	GCATC	4 cut(s)	79, 101, 396, 883
BpiI	GAAGAC	1 cut(s)	476
BpmI	CTGGAG	1 cut(s)	138
Bpu1102I	GCTNAGC	1 cut(s)	66
BpuEI	CTTGAG	1 cut(s)	221
Bsa29I	ATCGAT	2 cut(s)	258, 521
BsaAI	YACGTR	1 cut(s)	817
BsaJI	CCNNGG	2 cut(s)	166, 700
BsaXI	ACNNNNNCTCC	2 cut(s)	162, 192
Bse1I	ACTGG	2 cut(s)	121, 493
Bse21I	CCTNAGG	1 cut(s)	792
Bse3DI	GCAATG	1 cut(s)	877
BseBI	CCWGG	1 cut(s)	702
BseCI	ATCGAT	2 cut(s)	258, 521
BseDI	CCNNGG	2 cut(s)	166, 700
BseGI	GGATG	1 cut(s)	92
BseMI	GCAATG	1 cut(s)	877
BseMII	CTCAG	2 cut(s)	57, 806
BseNI	ACTGG	2 cut(s)	121, 493
BseRI	GAGGAG	1 cut(s)	271
BseXI	GCAGC	2 cut(s)	193, 812
BseYI	CCCAGC	1 cut(s)	884
BsgI	GTGCAG	2 cut(s)	617, 809
Bsh1236I	CGCG	1 cut(s)	168
BshFI	GGCC	1 cut(s)	37
BshVI	ATCGAT	2 cut(s)	258, 521
BsiHKAI	GWGCWC	1 cut(s)	648
BsmAI	GTCTC	3 cut(s)	137, 725, 869
BsmBI	CGTCTC	2 cut(s)	725, 869
BsmI	GAATGC	1 cut(s)	302
BsnI	GGCC	1 cut(s)	37
Bsp1286I	GDGCHC	1 cut(s)	648
Bsp143I	GATC	5 cut(s)	50, 328, 567, 574, 708
Bsp1720I	GCTNAGC	1 cut(s)	66
BspACI	CCGC	6 cut(s)	105, 166, 168, 189, 549, 849
BspANI	GGCC	1 cut(s)	37
BspCNI	CTCAG	2 cut(s)	58, 805
BspDI	ATCGAT	2 cut(s)	258, 521
BspFNI	CGCG	1 cut(s)	168
BspHI	TCATGA	1 cut(s)	723
BspMI	ACCTGC	1 cut(s)	61
BspPI	GGATC	3 cut(s)	58, 569, 575
BsrDI	GCAATG	1 cut(s)	877
BsrI	ACTGG	2 cut(s)	121, 493
BssECI	CCNNGG	2 cut(s)	166, 700
BssMI	GATC	5 cut(s)	50, 328, 567, 574, 708
Bst2UI	CCWGG	1 cut(s)	702
Bst4CI	ACNGT	2 cut(s)	478, 685
BstBAI	YACGTR	1 cut(s)	817
BstC8I	GCNNGC	1 cut(s)	636
BstDEI	CTNAG	2 cut(s)	66, 792
BstDSI	CCRYGG	1 cut(s)	166
BstF5I	GGATG	1 cut(s)	92
BstFNI	CGCG	1 cut(s)	168
BstHHI	GCGC	1 cut(s)	175
BstKTI	GATC	5 cut(s)	53, 331, 570, 577, 711
BstMAI	GTCTC	3 cut(s)	137, 725, 869
BstMBI	GATC	5 cut(s)	50, 328, 567, 574, 708
BstMWI	GCNNNNNNNGC	2 cut(s)	62, 215
BstNI	CCWGG	1 cut(s)	702
BstNSI	RCATGY	2 cut(s)	179, 778
BstSCI	CCNGG	1 cut(s)	700
BstSFI	CTRYAG	1 cut(s)	474
BstUI	CGCG	1 cut(s)	168
BstV1I	GCAGC	2 cut(s)	193, 812
BstV2I	GAAGAC	1 cut(s)	476
Bsu15I	ATCGAT	2 cut(s)	258, 521
Bsu36I	CCTNAGG	1 cut(s)	792
BsuRI	GGCC	1 cut(s)	37
BsuTUI	ATCGAT	2 cut(s)	258, 521
BtgI	CCRYGG	1 cut(s)	166
BtgZI	GCGATG	1 cut(s)	169
BtrI	CACGTC	1 cut(s)	719
BtsCI	GGATG	1 cut(s)	92
BtsI	GCAGTG	2 cut(s)	60, 821
BtsIMutI	CAGTG	2 cut(s)	60, 821
BveI	ACCTGC	1 cut(s)	61
Cac8I	GCNNGC	1 cut(s)	636
CciI	TCATGA	1 cut(s)	723
CfoI	GCGC	1 cut(s)	175
Cfr13I	GGNCC	1 cut(s)	35
Cfr42I	CCGCGG	1 cut(s)	169
ClaI	ATCGAT	2 cut(s)	258, 521
Csp6I	GTAC	2 cut(s)	788, 814
CviAII	CATG	3 cut(s)	176, 724, 775
CviQI	GTAC	2 cut(s)	788, 814
DdeI	CTNAG	2 cut(s)	66, 792
DpnI	GATC	5 cut(s)	52, 330, 569, 576, 710
DpnII	GATC	5 cut(s)	50, 328, 567, 574, 708
Ecl136II	GAGCTC	1 cut(s)	646
Eco24I	GRGCYC	1 cut(s)	648
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	1 cut(s)	99
Eco81I	CCTNAGG	1 cut(s)	792
EcoICRI	GAGCTC	1 cut(s)	646
EcoRII	CCWGG	1 cut(s)	700
EcoT22I	ATGCAT	2 cut(s)	94, 604
EcoT38I	GRGCYC	1 cut(s)	648
Esp3I	CGTCTC	2 cut(s)	725, 869
FaeI	CATG	3 cut(s)	179, 727, 778
FatI	CATG	3 cut(s)	175, 723, 774
FauI	CCCGC	1 cut(s)	542
FbaI	TGATCA	1 cut(s)	708
FblI	GTMKAC	1 cut(s)	864
Fnu4HI	GCNGC	3 cut(s)	189, 207, 801
FokI	GGATG	1 cut(s)	79
FriOI	GRGCYC	1 cut(s)	648
Fsp4HI	GCNGC	3 cut(s)	189, 207, 801
FspBI	CTAG	1 cut(s)	126
GlaI	GCGC	1 cut(s)	174
GluI	GCNGC	3 cut(s)	189, 207, 801
GsaI	CCCAGC	1 cut(s)	888
GsuI	CTGGAG	1 cut(s)	138
HaeIII	GGCC	1 cut(s)	37
HhaI	GCGC	1 cut(s)	175
Hin1II	CATG	3 cut(s)	179, 727, 778
Hin6I	GCGC	1 cut(s)	173
HinP1I	GCGC	1 cut(s)	173
HincII	GTYRAC	3 cut(s)	444, 688, 865
HindII	GTYRAC	3 cut(s)	444, 688, 865
HindIII	AAGCTT	1 cut(s)	920
HinfI	GANTC	2 cut(s)	255, 553
HpaI	GTTAAC	1 cut(s)	444
HphI	GGTGA	5 cut(s)	31, 94, 685, 757, 911
Hpy166II	GTNNAC	4 cut(s)	444, 688, 697, 865
Hpy188I	TCNGA	2 cut(s)	267, 391
Hpy188III	TCNNGA	4 cut(s)	326, 578, 712, 724
Hpy8I	GTNNAC	4 cut(s)	444, 688, 697, 865
Hpy99I	CGWCG	2 cut(s)	311, 657
HpyAV	CCTTC	7 cut(s)	21, 74, 388, 461, 539, 662, 742
HpyCH4III	ACNGT	2 cut(s)	478, 685
HpyCH4IV	ACGT	4 cut(s)	593, 718, 786, 816
HpyF10VI	GCNNNNNNNGC	2 cut(s)	62, 215
HpyF3I	CTNAG	2 cut(s)	66, 792
HpySE526I	ACGT	4 cut(s)	593, 718, 786, 816
Hsp92II	CATG	3 cut(s)	179, 727, 778
HspAI	GCGC	1 cut(s)	173
Ksp22I	TGATCA	1 cut(s)	708
KspAI	GTTAAC	1 cut(s)	444
KspI	CCGCGG	1 cut(s)	169
Kzo9I	GATC	5 cut(s)	50, 328, 567, 574, 708
LmnI	GCTCC	3 cut(s)	170, 797, 953
Lsp1109I	GCAGC	2 cut(s)	193, 812
LweI	GCATC	4 cut(s)	79, 101, 396, 883
MaeI	CTAG	1 cut(s)	126
MaeII	ACGT	4 cut(s)	593, 718, 786, 816
MaeIII	GTNAC	4 cut(s)	19, 73, 112, 584
MalI	GATC	5 cut(s)	52, 330, 569, 576, 710
MboI	GATC	5 cut(s)	50, 328, 567, 574, 708
MboII	GAAGA	4 cut(s)	404, 481, 677, 793
MhlI	GDGCHC	1 cut(s)	648
MluCI	AATT	4 cut(s)	410, 504, 511, 617
MmeI	TCCRAC	2 cut(s)	120, 120
Mph1103I	ATGCAT	2 cut(s)	94, 604
MseI	TTAA	5 cut(s)	234, 347, 443, 596, 756
MslI	CAYNNNNRTG	1 cut(s)	639
MspA1I	CMGCKG	1 cut(s)	168
MspR9I	CCNGG	1 cut(s)	702
Mva1269I	GAATGC	1 cut(s)	302
MvaI	CCWGG	1 cut(s)	702
MvnI	CGCG	1 cut(s)	168
MwoI	GCNNNNNNNGC	2 cut(s)	62, 215
NdeII	GATC	5 cut(s)	50, 328, 567, 574, 708
NlaIII	CATG	3 cut(s)	179, 727, 778
NmuCI	GTSAC	1 cut(s)	19
NsiI	ATGCAT	2 cut(s)	94, 604
NspI	RCATGY	2 cut(s)	179, 778
PagI	TCATGA	1 cut(s)	723
PctI	GAATGC	1 cut(s)	302
PfeI	GAWTC	2 cut(s)	255, 553
PkrI	GCNGC	3 cut(s)	190, 208, 802
Ppu21I	YACGTR	1 cut(s)	817
Psp124BI	GAGCTC	1 cut(s)	648
Psp6I	CCWGG	1 cut(s)	700
PspFI	CCCAGC	1 cut(s)	884
PspGI	CCWGG	1 cut(s)	700
PspPI	GGNCC	1 cut(s)	35
RsaI	GTAC	2 cut(s)	789, 815
RsaNI	GTAC	2 cut(s)	788, 814
RseI	CAYNNNNRTG	1 cut(s)	639
SacI	GAGCTC	1 cut(s)	648
SacII	CCGCGG	1 cut(s)	169
SalI	GTCGAC	1 cut(s)	863
SaqAI	TTAA	5 cut(s)	234, 347, 443, 596, 756
SatI	GCNGC	3 cut(s)	189, 207, 801
Sau3AI	GATC	5 cut(s)	50, 328, 567, 574, 708
Sau96I	GGNCC	1 cut(s)	35
ScrFI	CCNGG	1 cut(s)	702
SduI	GDGCHC	1 cut(s)	648
SfaNI	GCATC	4 cut(s)	79, 101, 396, 883
SfcI	CTRYAG	1 cut(s)	474
Sfr303I	CCGCGG	1 cut(s)	169
SgrBI	CCGCGG	1 cut(s)	169
SmiMI	CAYNNNNRTG	1 cut(s)	639
SmlI	CTYRAG	1 cut(s)	200
SmoI	CTYRAG	1 cut(s)	200
Sse9I	AATT	4 cut(s)	410, 504, 511, 617
SsiI	CCGC	6 cut(s)	105, 166, 168, 189, 549, 849
SspMI	CTAG	1 cut(s)	126
SstI	GAGCTC	1 cut(s)	648
StyD4I	CCNGG	1 cut(s)	700
TaaI	ACNGT	2 cut(s)	478, 685
TaiI	ACGT	4 cut(s)	596, 721, 789, 819
TaqI	TCGA	8 cut(s)	253, 258, 309, 327, 521, 556, 655, 864
TasI	AATT	4 cut(s)	410, 504, 511, 617
TauI	GCSGC	1 cut(s)	191
TfiI	GAWTC	2 cut(s)	255, 553
Tru1I	TTAA	5 cut(s)	234, 347, 443, 596, 756
Tru9I	TTAA	5 cut(s)	234, 347, 443, 596, 756
TscAI	CASTG	2 cut(s)	67, 828
TseFI	GTSAC	1 cut(s)	19
TseI	GCWGC	2 cut(s)	206, 800
Tsp45I	GTSAC	1 cut(s)	19
TspDTI	ATGAA	3 cut(s)	482, 761, 918
TspRI	CASTG	2 cut(s)	67, 828
XapI	RAATTY	1 cut(s)	511
XceI	RCATGY	2 cut(s)	179, 778
XmiI	GTMKAC	1 cut(s)	864
XspI	CTAG	1 cut(s)	126
Zsp2I	ATGCAT	2 cut(s)	94, 604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.