AT5G02700

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016476
Physical Location & Seq
Reverse (-)
609381 .. 611747
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G02700.1

Sequence Viewer

Length: 1371 bp
ATGGCGAAAAGGCGAGAGAGACGGGGCCGACGACAACATCGTAGCCACCGCAGAATCCAACGAATAATCGATGGTGCAGATTTCATCAACTATATGCCAGATGATATCCTTCACCATATTCTCTCCTTTATTCCCACCGATTTGGCCATGAGAACTTCCGTCTTATCCCGACGATGGAGGCATGTATGGTGCGAGACACCTTGTCTCGACATTACACTCAAGCACGGAGCGATGAACCAAACCCTAACTTCCTACACGGCTCCCATAATCACTAGTTTCAAACTTGTTATGGACCTTAACAGCAACACTGTACCCCAGGTTGATAGCTGGATCGAGTTTGCTCTCTCCCGCAATGTGCAAAATCTGTCTGTGTTCGTTAGGGATTTCACATACTCCAAGACTTACAGATTTCCAGATATCTTCTATCTCAGTTCCTCCTTAAAGCTACTCGACGTGACGTTGGACTTTTTTGATATGATTCCCACATGCACAGTGTCTTGGAAATCCCTAAGGAACTTAACACTGCGTTTTTGTCAAATTCCAGACGAATCCATTCATAATATTCTCTCTGGCTGTCCAATTCTCGAAAGCTTGACACTGGATACATGCAGATTACTTGAGCGTCTTGATCTGAGTAAATCACCGAATCTGAGGAGATTGGATATAAATCAGCAATATCGCCGCACAGGACCAGTTGCGATTGTTGCGCCACACATCTATTATTTGAGACTGACATACTCTAGCACACCATCTACTATAGTGGATGTCTCCTCTTTGAGCGAAGCTAACCTTACCATTATCAGTAGCCTACTCTCTCCCTTGACTGCAGATGGTTATCAAACCATGGCGCTGGAGATGCTATCAAAATTTCACAACGTTAAGAGGCTTACTGTTGGGGAAACCCTTCTTCAGATTCTATCTCTCGCCGAGCTCCGCGGTGTTCCTTTCCCAACGCTCAAGGTCCAAACTTTGACTGTTAAAACGGAGTTCGTTCGATCTGTAATCCCTGGTATATCAAGGTTACTACAGAATTCACCTGGATTAAAGAAGCTAAGACCATCTACAATGAAAATGCATCATTTAAAAGGTTTATATCCGGATCAATGTTGGAGATCAACATGTGAGGTGTTTCCTACCTCAAAGGAGATCTACAAGATGTTGGGTTGTAATGATGCAACGTTGAAACTCGTGGCTTCGTTTATGGATTTGGTGCTGAGAAACGCAAAGACGCTAGAGAGGATGGTTGTTTGGTTGGGAGGCATCTACTTTAATGGTGATGCACCATGGTTTGAAGAAGAGCTGTTTGACATGGTTGAAACACTTTCTCGCAACAATAATGTCTCCATTTTGCTCAAACAAAGCAATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_2

Protein Analysis

456

Amino Acids

52.42

Weight (kDa)

9.29

Isoelectric Point (pI)

55.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 32 - 64 8.3e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 97 - 269 4.5e-12 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 107 - 227 9.5e-14 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 936
AccIII TCCGGA 1 cut(s) 1096
AciI CCGC 5 cut(s) 49, 349, 682, 934, 936
AclI AACGTT 2 cut(s) 876, 1178
AclWI GGATC 2 cut(s) 338, 1107
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 3 cut(s) 537, 866, 1030
AcuI CTGAAG 1 cut(s) 893
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 1 cut(s) 174
AflIII ACRYGT 1 cut(s) 1118
AgsI TTSAA 4 cut(s) 280, 1183, 1292, 1316
AhdI GACNNNNNGTC 1 cut(s) 201
AhlI ACTAGT 1 cut(s) 272
AjiI CACGTC 1 cut(s) 454
AjnI CCWGG 3 cut(s) 315, 1006, 1036
AluBI AGCT 7 cut(s) 327, 445, 591, 785, 931, 1051, 1300
AluI AGCT 7 cut(s) 327, 445, 591, 785, 931, 1051, 1300
Alw21I GWGCWC 1 cut(s) 933
Alw26I GTCTC 6 cut(s) 13, 188, 209, 721, 772, 1345
AlwI GGATC 2 cut(s) 338, 1107
Aor13HI TCCGGA 1 cut(s) 1096
AoxI GGCC 2 cut(s) 25, 144
ApoI RAATTY 3 cut(s) 537, 866, 1030
Asp700I GAANNNNTTC 2 cut(s) 552, 903
AspLEI GCGC 2 cut(s) 709, 850
AspS9I GGNCC 4 cut(s) 25, 292, 689, 961
AsuHPI GGTGA 4 cut(s) 104, 633, 1026, 1286
AvaII GGWCC 3 cut(s) 292, 689, 961
AxyI CCTNAGG 1 cut(s) 509
BalI TGGCCA 1 cut(s) 146
BanII GRGCYC 1 cut(s) 933
BauI CACGAG 1 cut(s) 1187
Bbv12I GWGCWC 1 cut(s) 933
BccI CCATC 6 cut(s) 65, 168, 757, 824, 1066, 1234
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 3 cut(s) 317, 1008, 1038
BciVI GTATCC 1 cut(s) 595
BcoDI GTCTC 6 cut(s) 13, 188, 209, 721, 772, 1345
BcuI ACTAGT 1 cut(s) 272
BfaI CTAG 3 cut(s) 273, 741, 1232
BfmI CTRYAG 3 cut(s) 756, 825, 1025
BfoI RGCGCY 1 cut(s) 851
BfuI GTATCC 1 cut(s) 595
BglII AGATCT 1 cut(s) 1146
BisI GCNGC 1 cut(s) 682
BlsI GCNGC 1 cut(s) 683
Bme1390I CCNGG 3 cut(s) 317, 1008, 1038
Bme18I GGWCC 3 cut(s) 292, 689, 961
BmeRI GACNNNNNGTC 1 cut(s) 201
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 4 cut(s) 25, 292, 689, 961
BmiI GGNNCC 2 cut(s) 26, 261
BmrFI CCNGG 3 cut(s) 317, 1008, 1038
BmsI GCATC 5 cut(s) 846, 1084, 1162, 1267, 1269
BpmI CTGGAG 1 cut(s) 872
BpuEI CTTGAG 3 cut(s) 203, 638, 941
Bsa29I ATCGAT 1 cut(s) 69
BsaBI GATNNNNATC 2 cut(s) 666, 834
BsaJI CCNNGG 5 cut(s) 315, 843, 934, 1006, 1283
BsaWI WCCGGW 1 cut(s) 1096
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 2 cut(s) 603, 692
Bse21I CCTNAGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 358
Bse8I GATNNNNATC 2 cut(s) 666, 834
BseAI TCCGGA 1 cut(s) 1096
BseBI CCWGG 3 cut(s) 317, 1008, 1038
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 5 cut(s) 315, 843, 934, 1006, 1283
BseGI GGATG 2 cut(s) 769, 1245
BseJI GATNNNNATC 2 cut(s) 666, 834
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 4 cut(s) 442, 623, 641, 1205
BseNI ACTGG 2 cut(s) 603, 692
BseRI GAGGAG 2 cut(s) 667, 760
BsgI GTGCAG 1 cut(s) 96
Bsh1236I CGCG 1 cut(s) 936
BshFI GGCC 2 cut(s) 27, 146
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 933
BsiSI CCGG 1 cut(s) 1097
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 6 cut(s) 13, 188, 209, 721, 772, 1345
BsmBI CGTCTC 1 cut(s) 13
BsnI GGCC 2 cut(s) 27, 146
Bsp1286I GDGCHC 1 cut(s) 933
Bsp13I TCCGGA 1 cut(s) 1096
Bsp143I GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
Bsp19I CCATGG 2 cut(s) 843, 1283
BspACI CCGC 5 cut(s) 49, 349, 682, 934, 936
BspANI GGCC 2 cut(s) 27, 146
BspCNI CTCAG 4 cut(s) 441, 624, 642, 1206
BspDI ATCGAT 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 1096
BspFNI CGCG 1 cut(s) 936
BspLI GGNNCC 2 cut(s) 26, 261
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 2 cut(s) 338, 1107
BspQI GCTCTTC 1 cut(s) 1290
BsrDI GCAATG 1 cut(s) 358
BsrI ACTGG 2 cut(s) 603, 692
BssECI CCNNGG 5 cut(s) 315, 843, 934, 1006, 1283
BssMI GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 2 cut(s) 843, 1283
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 3 cut(s) 317, 1008, 1038
Bst4CI ACNGT 4 cut(s) 310, 493, 892, 976
Bst6I CTCTTC 1 cut(s) 1290
BstDEI CTNAG 6 cut(s) 428, 509, 632, 650, 1052, 1214
BstDSI CCRYGG 3 cut(s) 843, 934, 1283
BstF5I GGATG 2 cut(s) 769, 1245
BstFNI CGCG 1 cut(s) 936
BstH2I RGCGCY 1 cut(s) 851
BstHHI GCGC 2 cut(s) 709, 850
BstKTI GATC 6 cut(s) 333, 631, 998, 1102, 1115, 1149
BstMAI GTCTC 6 cut(s) 13, 188, 209, 721, 772, 1345
BstMBI GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
BstMWI GCNNNNNNNGC 2 cut(s) 704, 856
BstNI CCWGG 3 cut(s) 317, 1008, 1038
BstNSI RCATGY 4 cut(s) 185, 489, 609, 1122
BstSCI CCNGG 3 cut(s) 315, 1006, 1036
BstSFI CTRYAG 3 cut(s) 756, 825, 1025
BstUI CGCG 1 cut(s) 936
BstX2I RGATCY 1 cut(s) 1146
BstXI CCANNNNNNTGG 2 cut(s) 142, 850
BstYI RGATCY 1 cut(s) 1146
Bsu15I ATCGAT 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 509
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 2 cut(s) 27, 146
BsuTUI ATCGAT 1 cut(s) 69
BtgI CCRYGG 3 cut(s) 843, 934, 1283
BtgZI GCGATG 1 cut(s) 245
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 2 cut(s) 769, 1245
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 4 cut(s) 306, 498, 521, 596
CfoI GCGC 2 cut(s) 709, 850
Cfr13I GGNCC 4 cut(s) 25, 292, 689, 961
Cfr42I CCGCGG 1 cut(s) 937
ClaI ATCGAT 1 cut(s) 69
CseI GACGC 2 cut(s) 611, 1237
Csp6I GTAC 1 cut(s) 311
CviAII CATG 8 cut(s) 148, 182, 486, 606, 844, 1119, 1284, 1309
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 6 cut(s) 428, 509, 632, 650, 1052, 1214
DpnI GATC 6 cut(s) 332, 630, 997, 1101, 1114, 1148
DpnII GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
DraI TTTAAA 1 cut(s) 1083
DriI GACNNNNNGTC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 1290
Eam1105I GACNNNNNGTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 1290
Ecl136II GAGCTC 1 cut(s) 931
Eco130I CCWWGG 2 cut(s) 843, 1283
Eco24I GRGCYC 1 cut(s) 933
Eco32I GATATC 2 cut(s) 106, 418
Eco47I GGWCC 3 cut(s) 292, 689, 961
Eco53kI GAGCTC 1 cut(s) 931
Eco57I CTGAAG 1 cut(s) 893
Eco81I CCTNAGG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 931
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 3 cut(s) 315, 1006, 1036
EcoRV GATATC 2 cut(s) 106, 418
EcoT14I CCWWGG 2 cut(s) 843, 1283
EcoT22I ATGCAT 1 cut(s) 1077
EcoT38I GRGCYC 1 cut(s) 933
ErhI CCWWGG 2 cut(s) 843, 1283
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 8 cut(s) 151, 185, 489, 609, 847, 1122, 1287, 1312
FalI AAGNNNNNCTT 2 cut(s) 774, 806
FatI CATG 8 cut(s) 147, 181, 485, 605, 843, 1118, 1283, 1308
FauI CCCGC 1 cut(s) 356
Fnu4HI GCNGC 1 cut(s) 682
FokI GGATG 2 cut(s) 776, 1252
FriOI GRGCYC 1 cut(s) 933
Fsp4HI GCNGC 1 cut(s) 682
FspBI CTAG 3 cut(s) 273, 741, 1232
GlaI GCGC 2 cut(s) 708, 849
GluI GCNGC 1 cut(s) 682
GsuI CTGGAG 1 cut(s) 872
HaeII RGCGCY 1 cut(s) 851
HaeIII GGCC 2 cut(s) 27, 146
HapII CCGG 1 cut(s) 1097
HgaI GACGC 2 cut(s) 611, 1237
HhaI GCGC 2 cut(s) 709, 850
Hin1II CATG 8 cut(s) 151, 185, 489, 609, 847, 1122, 1287, 1312
Hin6I GCGC 2 cut(s) 707, 848
HinP1I GCGC 2 cut(s) 707, 848
HindIII AAGCTT 1 cut(s) 589
HinfI GANTC 5 cut(s) 54, 478, 548, 646, 913
HpaII CCGG 1 cut(s) 1097
HphI GGTGA 4 cut(s) 104, 633, 1026, 1286
Hpy188I TCNGA 3 cut(s) 633, 651, 912
Hpy188III TCNNGA 7 cut(s) 168, 206, 413, 542, 584, 626, 1097
Hpy99I CGWCG 3 cut(s) 33, 174, 455
HpyAV CCTTC 2 cut(s) 119, 914
HpyCH4III ACNGT 4 cut(s) 310, 493, 892, 976
HpyCH4IV ACGT 4 cut(s) 453, 458, 876, 1178
HpyCH4V TGCA 8 cut(s) 77, 358, 489, 609, 827, 1075, 1175, 1280
HpyF10VI GCNNNNNNNGC 2 cut(s) 704, 856
HpyF3I CTNAG 6 cut(s) 428, 509, 632, 650, 1052, 1214
HpySE526I ACGT 4 cut(s) 453, 458, 876, 1178
Hsp92II CATG 8 cut(s) 151, 185, 489, 609, 847, 1122, 1287, 1312
HspAI GCGC 2 cut(s) 707, 848
Kpn2I TCCGGA 1 cut(s) 1096
KspI CCGCGG 1 cut(s) 937
Kzo9I GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
LguI GCTCTTC 1 cut(s) 1290
LmnI GCTCC 3 cut(s) 227, 265, 936
LweI GCATC 5 cut(s) 846, 1084, 1162, 1267, 1269
MaeI CTAG 3 cut(s) 273, 741, 1232
MaeII ACGT 4 cut(s) 453, 458, 876, 1178
MaeIII GTNAC 2 cut(s) 454, 1020
MalI GATC 6 cut(s) 332, 630, 997, 1101, 1114, 1148
MboI GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
MboII GAAGA 4 cut(s) 412, 899, 1304, 1307
MfeI CAATTG 1 cut(s) 1363
MflI RGATCY 1 cut(s) 1146
MhlI GDGCHC 1 cut(s) 933
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 5 cut(s) 537, 579, 866, 1030, 1363
MluNI TGGCCA 1 cut(s) 146
MmeI TCCRAC 3 cut(s) 82, 441, 1088
MnlI CCTC 9 cut(s) 171, 445, 645, 781, 876, 1117, 1147, 1230, 1250
Mox20I TGGCCA 1 cut(s) 146
Mph1103I ATGCAT 1 cut(s) 1077
MroI TCCGGA 1 cut(s) 1096
MroXI GAANNNNTTC 2 cut(s) 552, 903
MscI TGGCCA 1 cut(s) 146
MseI TTAA 8 cut(s) 297, 440, 518, 879, 978, 1043, 1082, 1269
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 936
MspI CCGG 1 cut(s) 1097
MspR9I CCNGG 3 cut(s) 317, 1008, 1038
MunI CAATTG 1 cut(s) 1363
MvaI CCWGG 3 cut(s) 317, 1008, 1038
MvnI CGCG 1 cut(s) 936
MwoI GCNNNNNNNGC 2 cut(s) 704, 856
NcoI CCATGG 2 cut(s) 843, 1283
NdeII GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
NlaIII CATG 8 cut(s) 151, 185, 489, 609, 847, 1122, 1287, 1312
NlaIV GGNNCC 2 cut(s) 26, 261
NmeAIII GCCGAG 1 cut(s) 952
NmuCI GTSAC 1 cut(s) 454
NsiI ATGCAT 1 cut(s) 1077
NspI RCATGY 4 cut(s) 185, 489, 609, 1122
PciI ACATGT 1 cut(s) 1118
PciSI GCTCTTC 1 cut(s) 1290
PcsI WCGNNNNNNNCGW 2 cut(s) 28, 37
PdmI GAANNNNTTC 2 cut(s) 552, 903
PfeI GAWTC 5 cut(s) 54, 478, 548, 646, 913
PkrI GCNGC 1 cut(s) 683
PscI ACATGT 1 cut(s) 1118
Psp124BI GAGCTC 1 cut(s) 933
Psp1406I AACGTT 2 cut(s) 876, 1178
Psp6I CCWGG 3 cut(s) 315, 1006, 1036
PspGI CCWGG 3 cut(s) 315, 1006, 1036
PspN4I GGNNCC 2 cut(s) 26, 261
PspPI GGNCC 4 cut(s) 25, 292, 689, 961
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 1 cut(s) 1146
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SacI GAGCTC 1 cut(s) 933
SacII CCGCGG 1 cut(s) 937
SapI GCTCTTC 1 cut(s) 1290
SaqAI TTAA 8 cut(s) 297, 440, 518, 879, 978, 1043, 1082, 1269
SatI GCNGC 1 cut(s) 682
Sau3AI GATC 6 cut(s) 330, 628, 995, 1099, 1112, 1146
Sau96I GGNCC 4 cut(s) 25, 292, 689, 961
ScrFI CCNGG 3 cut(s) 317, 1008, 1038
SduI GDGCHC 1 cut(s) 933
SfaNI GCATC 5 cut(s) 846, 1084, 1162, 1267, 1269
SfcI CTRYAG 3 cut(s) 756, 825, 1025
Sfr303I CCGCGG 1 cut(s) 937
SgrBI CCGCGG 1 cut(s) 937
SinI GGWCC 3 cut(s) 292, 689, 961
SmlI CTYRAG 3 cut(s) 218, 617, 956
SmoI CTYRAG 3 cut(s) 218, 617, 956
SpeI ACTAGT 1 cut(s) 272
Sse9I AATT 5 cut(s) 537, 579, 866, 1030, 1363
SsiI CCGC 5 cut(s) 49, 349, 682, 934, 936
SspI AATATT 1 cut(s) 562
SspMI CTAG 3 cut(s) 273, 741, 1232
SstI GAGCTC 1 cut(s) 933
StyD4I CCNGG 3 cut(s) 315, 1006, 1036
StyI CCWWGG 2 cut(s) 843, 1283
TaaI ACNGT 4 cut(s) 310, 493, 892, 976
TaiI ACGT 4 cut(s) 456, 461, 879, 1181
TaqI TCGA 6 cut(s) 69, 207, 333, 450, 585, 994
TasI AATT 5 cut(s) 537, 579, 866, 1030, 1363
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 5 cut(s) 54, 478, 548, 646, 913
Tru1I TTAA 8 cut(s) 297, 440, 518, 879, 978, 1043, 1082, 1269
Tru9I TTAA 8 cut(s) 297, 440, 518, 879, 978, 1043, 1082, 1269
TscAI CASTG 4 cut(s) 313, 498, 528, 603
TseFI GTSAC 1 cut(s) 454
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 73, 248, 545, 1082
TspGWI ACGGA 3 cut(s) 148, 240, 998
TspRI CASTG 4 cut(s) 313, 498, 528, 603
VpaK11BI GGWCC 3 cut(s) 292, 689, 961
XapI RAATTY 3 cut(s) 537, 866, 1030
XceI RCATGY 4 cut(s) 185, 489, 609, 1122
XmnI GAANNNNTTC 2 cut(s) 552, 903
XspI CTAG 3 cut(s) 273, 741, 1232
Zsp2I ATGCAT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.