AT5G03100

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016476
Physical Location & Seq
Forward (+)
726779 .. 728855
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03100.1

Sequence Viewer

Length: 924 bp
ATGAAACGAGCCGGCGGCGGTGTCGACTTCATCAGCTCTCTACCCGACGAAATCCTCCACCATATCCTCGCCAATACACCTACCAAGCTCGCGATCAGAACCTCCGTCTTATCCAAACGATGGAAGCATGTATGGTACGAGACACCTTCTATCTCAATTGTTTGCAATAGAGTAGATCCTGATTCGCTAAACAAAACCCTAAGTAGCTACTCGACTCCCAAAATCAAGAGTTTCGATGTTACTATAAGCAGAGATGTCACCGTACCTGAGATTGATACCTGGATTAATCTCGCTCTGTCACGCAAGGCTGAGAATGTATCTCTCAGATTCACATCTCATTATAGATTCCGAGATACATTCTTCATCAATTCCTCTCTGAAGCAGCTTAGTTTGACTTTGGTTTATTGCATTCTGAATCCCAAGTGCGTTGTCTCTTGGTCATCGTTAAGGAACTTGTCGTTGAATCGTTGCAAGGTGTCTGATGATTCAATTGCCAAAATTCTCACTGGTTGTTCATTGCTCGAAAGCTTGACATTGAATCTCTGTGATAGACTCAATGATCTTGATCTGAGCAAGTCACTAAGTTTGAGAAGACTGGAGATACTTGGAGACCGTTGGACACCAGAGAGGATTGTAGCGCCACACATTCGTTATTTGAGATTGGAAAACTATCAGCGACCATCTACTTTAGTGGATGTCTCATCTTTAACCGAAGCTAATCTCGGTCTTTCAAAACATGTACTTGACTATTTCACATGTGAAATGGAGACTGAATCGCTTCAATACATGGTGCGACAGACGGTGGTTAAGTTGCAAAACATAAAGAAGCTTACTATTGGGGGAATTTTTCTTCAGATTCTATCTCTTGCCGAGCTCTGTGGTGTTACTCTTCCAGTCTTCCAGAATTCAATATCGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

307

Amino Acids

34.84

Weight (kDa)

9.16

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 11 - 45 2e-07 F-box domain
LRR_At1g61320_AtMIF1 PF23622 74 - 202 2.6e-10 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 92 - 202 4.1e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AccI GTMKAC 1 cut(s) 24
AccII CGCG 1 cut(s) 92
AciI CCGC 2 cut(s) 15, 18
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 3 cut(s) 498, 843, 904
AcuI CTGAAG 2 cut(s) 398, 836
AfaI GTAC 3 cut(s) 137, 264, 741
AfiI CCNNNNNNNGG 1 cut(s) 120
AflIII ACRYGT 2 cut(s) 736, 755
AgsI TTSAA 6 cut(s) 463, 489, 538, 732, 782, 909
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 9 cut(s) 36, 88, 207, 385, 528, 716, 829, 874, 919
AluI AGCT 9 cut(s) 36, 88, 207, 385, 528, 716, 829, 874, 919
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 5 cut(s) 134, 436, 603, 703, 761
AlwI GGATC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 3 cut(s) 498, 843, 904
AseI ATTAAT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 777
AspLEI GCGC 1 cut(s) 640
AsuHPI GGTGA 1 cut(s) 250
BanII GRGCYC 1 cut(s) 876
BbsI GAAGAC 2 cut(s) 598, 889
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 1 cut(s) 394
BccI CCATC 2 cut(s) 114, 688
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 5 cut(s) 134, 436, 603, 703, 761
BfoI RGCGCY 1 cut(s) 641
BisI GCNGC 2 cut(s) 16, 383
BlsI GCNGC 2 cut(s) 17, 384
Bme1390I CCNGG 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 280
BpiI GAAGAC 2 cut(s) 598, 889
BpmI CTGGAG 1 cut(s) 617
BsaBI GATNNNNATC 2 cut(s) 331, 564
BsaI GGTCTC 1 cut(s) 603
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 3 cut(s) 511, 600, 893
Bse3DI GCAATG 1 cut(s) 515
Bse8I GATNNNNATC 2 cut(s) 331, 564
BseBI CCWGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 700
BseJI GATNNNNATC 2 cut(s) 331, 564
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 4 cut(s) 258, 300, 337, 560
BseNI ACTGG 3 cut(s) 511, 600, 893
BseXI GCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 876
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 5 cut(s) 134, 436, 603, 703, 761
BsmI GAATGC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 603
Bsp1286I GDGCHC 1 cut(s) 876
Bsp143I GATC 4 cut(s) 93, 175, 559, 565
Bsp68I TCGCGA 1 cut(s) 92
BspACI CCGC 2 cut(s) 15, 18
BspCNI CTCAG 4 cut(s) 259, 301, 336, 561
BspFNI CGCG 1 cut(s) 92
BspPI GGATC 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 515
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 3 cut(s) 511, 600, 893
BssAI RCCGGY 1 cut(s) 11
BssMI GATC 4 cut(s) 93, 175, 559, 565
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 3 cut(s) 262, 614, 802
Bst6I CTCTTC 1 cut(s) 894
BstC8I GCNNGC 2 cut(s) 13, 90
BstDEI CTNAG 7 cut(s) 200, 267, 309, 323, 386, 569, 581
BstF5I GGATG 1 cut(s) 700
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 1 cut(s) 640
BstKTI GATC 4 cut(s) 96, 178, 562, 568
BstMAI GTCTC 5 cut(s) 134, 436, 603, 703, 761
BstMBI GATC 4 cut(s) 93, 175, 559, 565
BstNI CCWGG 1 cut(s) 280
BstNSI RCATGY 3 cut(s) 131, 740, 759
BstSCI CCNGG 1 cut(s) 278
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 2 cut(s) 598, 889
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BtsCI GGATG 1 cut(s) 700
BtsIMutI CAGTG 1 cut(s) 504
BtuMI TCGCGA 1 cut(s) 92
Cac8I GCNNGC 2 cut(s) 13, 90
CfoI GCGC 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 11
Csp6I GTAC 3 cut(s) 136, 263, 740
CviAII CATG 4 cut(s) 128, 737, 756, 787
CviQI GTAC 3 cut(s) 136, 263, 740
DdeI CTNAG 7 cut(s) 200, 267, 309, 323, 386, 569, 581
DpnI GATC 4 cut(s) 95, 177, 561, 567
DpnII GATC 4 cut(s) 93, 175, 559, 565
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 874
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 603
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 2 cut(s) 398, 836
EcoICRI GAGCTC 1 cut(s) 874
EcoRI GAATTC 1 cut(s) 904
EcoRII CCWGG 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 876
FaeI CATG 4 cut(s) 131, 740, 759, 790
FaiI YATR 9 cut(s) 63, 129, 133, 245, 342, 738, 757, 788, 821
FatI CATG 4 cut(s) 127, 736, 755, 786
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 16, 383
FokI GGATG 1 cut(s) 707
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 2 cut(s) 16, 383
GlaI GCGC 1 cut(s) 639
GluI GCNGC 2 cut(s) 16, 383
GsuI CTGGAG 1 cut(s) 617
HaeII RGCGCY 1 cut(s) 641
HapII CCGG 1 cut(s) 12
HhaI GCGC 1 cut(s) 640
Hin1II CATG 4 cut(s) 131, 740, 759, 790
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 3 cut(s) 526, 827, 917
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 8 cut(s) 98, 326, 350, 378, 414, 481, 570, 855
Hpy188III TCNNGA 5 cut(s) 91, 179, 226, 563, 901
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 3 cut(s) 262, 614, 802
HpyCH4V TGCA 4 cut(s) 165, 408, 471, 814
HpyF3I CTNAG 7 cut(s) 200, 267, 309, 323, 386, 569, 581
Hsp92II CATG 4 cut(s) 131, 740, 759, 790
HspAI GCGC 1 cut(s) 638
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Kzo9I GATC 4 cut(s) 93, 175, 559, 565
Lsp1109I GCAGC 1 cut(s) 394
MaeIII GTNAC 5 cut(s) 238, 256, 297, 576, 883
MalI GATC 4 cut(s) 95, 177, 561, 567
MboI GATC 4 cut(s) 93, 175, 559, 565
MboII GAAGA 5 cut(s) 352, 603, 842, 881, 889
MfeI CAATTG 2 cut(s) 156, 489
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 876
MluCI AATT 6 cut(s) 156, 367, 489, 498, 843, 904
MlyI GAGTC 2 cut(s) 208, 546
MmeI TCCRAC 1 cut(s) 596
MnlI CCTC 5 cut(s) 65, 77, 112, 382, 621
MroNI GCCGGC 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 777
MseI TTAA 4 cut(s) 285, 446, 707, 807
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 280
MunI CAATTG 2 cut(s) 156, 489
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 92
NaeI GCCGGC 1 cut(s) 13
NdeII GATC 4 cut(s) 93, 175, 559, 565
NgoMIV GCCGGC 1 cut(s) 11
NlaIII CATG 4 cut(s) 131, 740, 759, 790
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 3 cut(s) 256, 297, 576
NruI TCGCGA 1 cut(s) 92
NspI RCATGY 3 cut(s) 131, 740, 759
PciI ACATGT 2 cut(s) 736, 755
PctI GAATGC 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 9 cut(s) 182, 327, 345, 415, 463, 485, 538, 773, 856
PflMI CCANNNNNTGG 1 cut(s) 120
PkrI GCNGC 2 cut(s) 17, 384
PleI GAGTC 2 cut(s) 208, 546
PpsI GAGTC 2 cut(s) 208, 546
PscI ACATGT 2 cut(s) 736, 755
PshBI ATTAAT 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 876
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 175
RruI TCGCGA 1 cut(s) 92
RsaI GTAC 3 cut(s) 137, 264, 741
RsaNI GTAC 3 cut(s) 136, 263, 740
SacI GAGCTC 1 cut(s) 876
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 4 cut(s) 285, 446, 707, 807
SatI GCNGC 2 cut(s) 16, 383
Sau3AI GATC 4 cut(s) 93, 175, 559, 565
SchI GAGTC 2 cut(s) 208, 546
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 1 cut(s) 876
Sse9I AATT 6 cut(s) 156, 367, 489, 498, 843, 904
SsiI CCGC 2 cut(s) 15, 18
SstI GAGCTC 1 cut(s) 876
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 3 cut(s) 262, 614, 802
TaqI TCGA 5 cut(s) 24, 212, 234, 522, 914
TaqII GACCGA 1 cut(s) 713
TasI AATT 6 cut(s) 156, 367, 489, 498, 843, 904
TatI WGTACW 1 cut(s) 739
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 9 cut(s) 182, 327, 345, 415, 463, 485, 538, 773, 856
Tru1I TTAA 4 cut(s) 285, 446, 707, 807
Tru9I TTAA 4 cut(s) 285, 446, 707, 807
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 3 cut(s) 256, 297, 576
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 3 cut(s) 256, 297, 576
TspDTI ATGAA 4 cut(s) 17, 19, 352, 504
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 511
Van91I CCANNNNNTGG 1 cut(s) 120
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 3 cut(s) 498, 843, 904
XceI RCATGY 3 cut(s) 131, 740, 759
XmiI GTMKAC 1 cut(s) 24
XmnI GAANNNNTTC 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.