Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007372

Physical Location & Seq

Genomic Coordinates Forward (+)

1089342 .. 1093204

Gene Structure 3863 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G04040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGGATATAAGTAATGAGGCTAGTGTCGATCCCTTTTCGATTGGACCATCATCTATCATGGGTCGAACCATTGCTTTCAGAGTCTTGTTCTGTAGATCAATGTCACAGCTTAGGCGTGATCTCTTTCGGTTCTTGTTGCATTGGTTTCTTAGATTTAAGCTGACCGTTTCACCGTTTGTGTCGTGGTTTCATCCTCGGAACCCTCAAGGGATTTTAGCGGTGGTTACAATCATTGCCTTTGTGTTGAAACGATACACGAATGTGAAAATAAAGGCGGAAATGGCTTACCGGAGGAAGTTTTGGAGGAATATGATGCGGACGGCTTTGACTTATGAGGAATGGGCTCATGCTGCTAAGATGTTAGAGAAGGAAACACCAAAGATGAATGAATCTGATCTTTATGATGAAGAGTTGGTTAAGAACAAGCTTCAGGAGCTTCGTCATCGTCGCCAAGAAGGCTCACTTAGAGACATTATGTTTTGTATGAGAGCTGATTTGGTGAGGAATCTCGGTAATATGTGTAATTCGGAGCTTCATAAAGGTAGACTTCAGGTTCCTAGACATATCAAAGAGTACATTGATGAGGTGTCTACTCAGTTGAGAATGGTTTGTAACTCTGATTCAGAGGAGCTTTCTTTAGAAGAGAAGCTTTCTTTTATGCATGAAACACGGCATGCCTTTGGTAGAACGGCTTTGCTTTTGAGTGGTGGGGCTTCTCTTGGTGCGTTTCATGTTGGTGTGGTTAGGACTTTGGTTGAGCATAAGCTTTTACCTCGAATAATTGCTGGTTCTAGTGTTGGATCCATCATTTGTGCTGTTGTGGCCTCAAGGTCTTGGCCAGAACTACAGAGTTTCTTTGAGAATTCTTTGCATTCTTTACAGTTCTTTGATCAGCTCGGAGGCGTGTTCTCAATAGTGAAACGGGTAATGACACAAGGGGCTCTACACGATATCAGACAGTTGCAATGTATGCTTAGAAACCTCACAAGCAATCTCACATTCCAAGAAGCTTATGACATGACAGGAAGGATTCTCGGGATCACCGTTTGCTCCCCAAGAAAGCATGAACCTCCTCGGTGTCTTAACTATTTGACTTCGCCTCATGTGGTTATATGGAGCGCAGTGACTGCTTCTTGTGCTTTTCCTGGTCTCTTTGAAGCTCAAGAGCTAATGGCTAAAGATCGAAGTGGAGAGATCGTACCGTATCATCCACCTTTCAATTTGGATCCAGAAGTAGGCACTAAATCATCATCTGGACGCCGGTGGAGAGATGGTAGTTTGGAGGTTGATTTACCAATGATGCAGCTTAAAGAACTGTTCAATGTCAATCATTTTATTGTGAGCCAAGCCAATCCTCACATTGCTCCATTACTGCGTCTAAAGGATTTAGTTCGAGCTTATGGTGGTAGATTCGCAGCTAAGCTCGCGCATCTAGTGGAGATGGAGGTCAAACATAGATGCAACCAGGTATTAGAGCTCGGTTTTCCTCTCGGTGGACTCGCAAAGCTTTTTGCTCAGGAGTGGGAAGGTGATGTTACAGTTGTAATGCCTGCTACTCTTGCTCAGTACTCGAAGATTATACAAAATCCGACTCATGTCGAGCTTCAGAAAGCGGCTAACCAAGGAAGAAGATGCACTTGGGAGAAGCTCTCAGCCATAAAATCAAACTGCGGGATCGAGCTTGCGCTTGATGATTCTGTAGCTATTCTTAACCATATGCGGAGGCTCAAGAAAAGTGCGGAGAGAGCCGCCACTGCCACGTCTTCGTCTCATCACGGATTGGCTTCAACCACCAGATTCAATGCTTCAAGAAGAATCCCATCTTGGAACGTCCTTGCCAGAGAGAACTCAACAGGCTCACTGGATGATCTAGTCACTGACAATAACCTCCACGCTTCTTCGGGCAGGAATTTAAGCGACAGTGAAACAGAGAGCGTGGAGTTGAGTTCTTGGACAAGAACTGGTGGACCTTTAATGAGAACAGCTTCTGCTAATAAGTTCATTGATTTTGTTCAGAGTCTTGATATCGACATTGCATTGGTCAGAGGATTTAGTAGCAGTCCCAATTCTCCAGCAGTTCCTCCTGGTGGCTCGTTTACTCCAAGCCCGAGATCCATAGCGGCTCATTCGGATATCGAATCAAACAGCAATAGCAACAATCTTGGAACAAGCACTTCAAGCATAACAGTTACTGAAGGTGATCTTCTACAGCCTGAGAGAACGAGTAACGGATTTGTGTTAAACGTCGTTAAAAGAGAGAACTTGGGAATGCCATCGATTGGGAACCAAAATACAGAGTTACCAGAGAGTGTACAGCTCGATATACCGGAGAAGGAGATGGATTGTAGCTCTGTATCAGAACACGAAGAAGATGATAACGACAATGAAGAAGAACATAACGGCTCGAGTCTGGTTACTGTTTCTTCAGAAGATTCCGGTTTACAAGAACCGGTGTCTGGTAGTGTTATAGATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005811 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0012511 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019433 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044464 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0052689 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00100 ko00561 ko00564 ko00565 ko00590 ko00591 ko00592 ko01100 ko01110 map00100 map00561 map00564 map00565 map00590 map00591 map00592 map01100 map01110

Pfam Domains

Protein Families

DUF3336 Patatin

Protein Analysis

825

Amino Acids

92.09

Weight (kDa)

6.55

Isoelectric Point (pI)

50.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3336	PF11815	94 - 225	8e-31	Domain of unknown function (DUF3336)
Patatin	PF01734	232 - 390	1.1e-20	Patatin-like phospholipase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007372)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04040 AT5G04040
fragaria_vesca	FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040
malus_domestica	MD12G1087400.v1.1 MD14G1083100.v1.1
prunus_persica	Prupe.7G047400_v2.0.a1
pyrus_communis	pycom12g07540 pycom14g07220
rosa_chinensis	RchiOBHm_Chr2g0124811
rosa_laevigata	RLG00000018823
rosa_multiflora	Rmu_sc0002939.1_g000008
rosa_roxburghii	Rroxscaffold_2G00119660
rosa_rugosa	Rorug02G0252600 Rorug02G0252700 Rorug02G0252800
rosa_samantha	Rh2AG312300 Rh2BG320500 Rh2CG300500 Rh2DG337700
rosa_wichuraiana	Rw2G025220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2279
AccI	GTMKAC	2 cut(s)	544, 590
AccII	CGCG	1 cut(s)	1427
AciI	CCGC	9 cut(s)	218, 275, 316, 1613, 1671, 1720, 1739, 1749, 2120
AclWI	GGATC	8 cut(s)	23, 795, 808, 1046, 1220, 1233, 1682, 2106
AcoI	YGGCCR	1 cut(s)	836
AcsI	RAATTY	2 cut(s)	862, 1909
AcuI	CTGAAG	5 cut(s)	413, 533, 1589, 2214, 2409
AcyI	GRCGYC	1 cut(s)	1258
AfaI	GTAC	4 cut(s)	575, 1200, 1568, 2313
AfiI	CCNNNNNNNGG	5 cut(s)	1235, 1493, 2087, 2279, 2456
AgeI	ACCGGT	1 cut(s)	2449
AgsI	TTSAA	8 cut(s)	247, 1157, 1219, 1321, 1788, 1801, 1809, 2178
AjiI	CACGTC	1 cut(s)	1761
AjnI	CCWGG	3 cut(s)	1144, 1464, 2083
AjuI	GAANNNNNNNTTGG	4 cut(s)	1621, 1653, 1807, 1839
AleI	CACNNNNGTG	1 cut(s)	260
Alw21I	GWGCWC	1 cut(s)	1479
Alw26I	GTCTC	3 cut(s)	462, 1154, 1773
AlwI	GGATC	8 cut(s)	23, 795, 808, 1046, 1220, 1233, 1682, 2106
AlwNI	CAGNNNCTG	3 cut(s)	1127, 1988, 2192
Ama87I	CYCGRG	3 cut(s)	1034, 2107, 2404
AoxI	GGCC	2 cut(s)	822, 836
ApeKI	GCWGC	3 cut(s)	350, 1303, 1415
ApoI	RAATTY	2 cut(s)	862, 1909
AsiGI	ACCGGT	1 cut(s)	2449
Asp700I	GAANNNNTTC	1 cut(s)	1984
AspLEI	GCGC	3 cut(s)	1121, 1429, 1687
AspS9I	GGNCC	2 cut(s)	44, 1967
AsuHPI	GGTGA	5 cut(s)	162, 511, 1033, 1541, 2210
AvaI	CYCGRG	3 cut(s)	1034, 2107, 2404
AvaII	GGWCC	2 cut(s)	44, 1967
BalI	TGGCCA	1 cut(s)	838
BamHI	GGATCC	2 cut(s)	800, 1225
BanII	GRGCYC	3 cut(s)	346, 943, 1479
BbsI	GAAGAC	1 cut(s)	1755
Bbv12I	GWGCWC	1 cut(s)	1479
BbvI	GCAGC	3 cut(s)	337, 1315, 1427
BccI	CCATC	7 cut(s)	55, 812, 1265, 1435, 1828, 2281, 2332
BceAI	ACGGC	4 cut(s)	336, 686, 705, 2416
BciT130I	CCWGG	3 cut(s)	1146, 1466, 2085
BclI	TGATCA	1 cut(s)	889
BcoDI	GTCTC	3 cut(s)	462, 1154, 1773
BfaI	CTAG	5 cut(s)	21, 558, 792, 1433, 1871
BfmI	CTRYAG	4 cut(s)	91, 845, 1698, 2207
BglI	GCCNNNNNGGC	1 cut(s)	456
BisI	GCNGC	6 cut(s)	351, 1304, 1416, 1614, 1749, 2121
BlpI	GCTNAGC	1 cut(s)	1419
BlsI	GCNGC	6 cut(s)	352, 1305, 1417, 1615, 1750, 2122
BmcAI	AGTACT	1 cut(s)	1568
Bme1390I	CCNGG	3 cut(s)	1146, 1466, 2085
Bme18I	GGWCC	2 cut(s)	44, 1967
BmeT110I	CYCGRG	3 cut(s)	1034, 2107, 2404
BmgBI	CACGTC	1 cut(s)	1761
BmgT120I	GGNCC	2 cut(s)	44, 1967
BmiI	GGNNCC	5 cut(s)	200, 555, 802, 1227, 2285
BmrFI	CCNGG	3 cut(s)	1146, 1466, 2085
BmsI	GCATC	6 cut(s)	303, 1290, 1438, 1448, 1622, 2461
BoxI	GACNNNNGTC	1 cut(s)	1595
BpiI	GAAGAC	1 cut(s)	1755
BplI	GAGNNNNNCTC	2 cut(s)	1634, 1666
BpmI	CTGGAG	1 cut(s)	2055
Bpu10I	CCTNAGC	2 cut(s)	110, 1515
Bpu1102I	GCTNAGC	1 cut(s)	1419
BpuEI	CTTGAG	4 cut(s)	189, 811, 1146, 1712
Bsa29I	ATCGAT	1 cut(s)	2276
BsaHI	GRCGYC	1 cut(s)	1258
BsaI	GGTCTC	1 cut(s)	1154
BsaJI	CCNNGG	3 cut(s)	194, 1073, 1621
BsaWI	WCCGGW	4 cut(s)	288, 2326, 2435, 2449
BsaXI	ACNNNNNCTCC	4 cut(s)	1182, 1212, 1258, 1288
Bsc4I	CCNNNNNNNGG	5 cut(s)	1235, 1493, 2087, 2279, 2456
Bse118I	RCCGGY	2 cut(s)	1260, 2449
Bse1I	ACTGG	2 cut(s)	1866, 1966
Bse3DI	GCAATG	5 cut(s)	69, 231, 971, 1359, 2031
BseBI	CCWGG	3 cut(s)	1146, 1466, 2085
BseCI	ATCGAT	1 cut(s)	2276
BseDI	CCNNGG	3 cut(s)	194, 1073, 1621
BseGI	GGATG	3 cut(s)	190, 1207, 1870
BseLI	CCNNNNNNNGG	5 cut(s)	1235, 1493, 2087, 2279, 2456
BseMI	GCAATG	5 cut(s)	69, 231, 971, 1359, 2031
BseMII	CTCAG	5 cut(s)	608, 1529, 1577, 1665, 2205
BseNI	ACTGG	2 cut(s)	1866, 1966
BseRI	GAGGAG	2 cut(s)	641, 1062
BseXI	GCAGC	3 cut(s)	337, 1315, 1427
Bsh1236I	CGCG	1 cut(s)	1427
BshFI	GGCC	2 cut(s)	824, 838
BshTI	ACCGGT	1 cut(s)	2449
BshVI	ATCGAT	1 cut(s)	2276
BsiHKAI	GWGCWC	1 cut(s)	1479
BsiHKCI	CYCGRG	3 cut(s)	1034, 2107, 2404
BsiSI	CCGG	5 cut(s)	289, 1261, 2327, 2436, 2450
BslFI	GGGAC	1 cut(s)	2046
BslI	CCNNNNNNNGG	5 cut(s)	1235, 1493, 2087, 2279, 2456
BsmAI	GTCTC	3 cut(s)	462, 1154, 1773
BsmBI	CGTCTC	1 cut(s)	1773
BsmFI	GGGAC	1 cut(s)	2046
BsmI	GAATGC	2 cut(s)	871, 2274
BsnI	GGCC	2 cut(s)	824, 838
Bso31I	GGTCTC	1 cut(s)	1154
BsoBI	CYCGRG	3 cut(s)	1034, 2107, 2404
Bsp1286I	GDGCHC	3 cut(s)	346, 943, 1479
Bsp1407I	TGTACA	1 cut(s)	2311
Bsp1720I	GCTNAGC	1 cut(s)	1419
BspACI	CCGC	9 cut(s)	218, 275, 316, 1613, 1671, 1720, 1739, 1749, 2120
BspANI	GGCC	2 cut(s)	824, 838
BspCNI	CTCAG	5 cut(s)	607, 1528, 1576, 1664, 2206
BspDI	ATCGAT	1 cut(s)	2276
BspFNI	CGCG	1 cut(s)	1427
BspLI	GGNNCC	5 cut(s)	200, 555, 802, 1227, 2285
BspPI	GGATC	8 cut(s)	23, 795, 808, 1046, 1220, 1233, 1682, 2106
BspTNI	GGTCTC	1 cut(s)	1154
BsrDI	GCAATG	5 cut(s)	69, 231, 971, 1359, 2031
BsrFI	RCCGGY	2 cut(s)	1260, 2449
BsrGI	TGTACA	1 cut(s)	2311
BsrI	ACTGG	2 cut(s)	1866, 1966
BssAI	RCCGGY	2 cut(s)	1260, 2449
BssECI	CCNNGG	3 cut(s)	194, 1073, 1621
BssNI	GRCGYC	1 cut(s)	1258
BssT1I	CCWWGG	1 cut(s)	1621
Bst2UI	CCWGG	3 cut(s)	1146, 1466, 2085
Bst6I	CTCTTC	2 cut(s)	402, 636
BstACI	GRCGYC	1 cut(s)	1258
BstAPI	GCANNNNNTGC	2 cut(s)	970, 1127
BstAUI	TGTACA	1 cut(s)	2311
BstC8I	GCNNGC	4 cut(s)	675, 1425, 1551, 1683
BstF5I	GGATG	3 cut(s)	190, 1207, 1870
BstFNI	CGCG	1 cut(s)	1427
BstHHI	GCGC	3 cut(s)	1121, 1429, 1687
BstMAI	GTCTC	3 cut(s)	462, 1154, 1773
BstNI	CCWGG	3 cut(s)	1146, 1466, 2085
BstNSI	RCATGY	1 cut(s)	677
BstPAI	GACNNNNGTC	1 cut(s)	1595
BstSCI	CCNGG	3 cut(s)	1144, 1464, 2083
BstSFI	CTRYAG	4 cut(s)	91, 845, 1698, 2207
BstUI	CGCG	1 cut(s)	1427
BstV1I	GCAGC	3 cut(s)	337, 1315, 1427
BstV2I	GAAGAC	1 cut(s)	1755
BstX2I	RGATCY	3 cut(s)	800, 1225, 2111
BstYI	RGATCY	3 cut(s)	800, 1225, 2111
Bsu15I	ATCGAT	1 cut(s)	2276
BsuRI	GGCC	2 cut(s)	824, 838
BsuTUI	ATCGAT	1 cut(s)	2276
BtrI	CACGTC	1 cut(s)	1761
BtsCI	GGATG	3 cut(s)	190, 1207, 1870
BtsI	GCAGTG	2 cut(s)	1128, 1752
BtsIMutI	CAGTG	5 cut(s)	1128, 1752, 1859, 1875, 1927
Cac8I	GCNNGC	4 cut(s)	675, 1425, 1551, 1683
CaiI	CAGNNNCTG	3 cut(s)	1127, 1988, 2192
CfoI	GCGC	3 cut(s)	1121, 1429, 1687
Cfr10I	RCCGGY	2 cut(s)	1260, 2449
Cfr13I	GGNCC	2 cut(s)	44, 1967
ClaI	ATCGAT	1 cut(s)	2276
CseI	GACGC	2 cut(s)	1266, 1364
CsiI	ACCWGGT	1 cut(s)	1464
Csp6I	GTAC	4 cut(s)	574, 1199, 1567, 2312
CspAI	ACCGGT	1 cut(s)	2449
CviAII	CATG	9 cut(s)	58, 347, 662, 674, 731, 1018, 1064, 1103, 1595
CviQI	GTAC	4 cut(s)	574, 1199, 1567, 2312
EaeI	YGGCCR	1 cut(s)	836
Eam1104I	CTCTTC	2 cut(s)	402, 636
EarI	CTCTTC	2 cut(s)	402, 636
EciI	GGCGGA	1 cut(s)	290
Ecl136II	GAGCTC	1 cut(s)	1477
Eco130I	CCWWGG	1 cut(s)	1621
Eco24I	GRGCYC	3 cut(s)	346, 943, 1479
Eco31I	GGTCTC	1 cut(s)	1154
Eco32I	GATATC	3 cut(s)	952, 2026, 2134
Eco47I	GGWCC	2 cut(s)	44, 1967
Eco53kI	GAGCTC	1 cut(s)	1477
Eco57I	CTGAAG	5 cut(s)	413, 533, 1589, 2214, 2409
Eco88I	CYCGRG	3 cut(s)	1034, 2107, 2404
EcoICRI	GAGCTC	1 cut(s)	1477
EcoRI	GAATTC	1 cut(s)	862
EcoRII	CCWGG	3 cut(s)	1144, 1464, 2083
EcoRV	GATATC	3 cut(s)	952, 2026, 2134
EcoT14I	CCWWGG	1 cut(s)	1621
EcoT22I	ATGCAT	1 cut(s)	663
EcoT38I	GRGCYC	3 cut(s)	346, 943, 1479
ErhI	CCWWGG	1 cut(s)	1621
Esp3I	CGTCTC	1 cut(s)	1773
FaeI	CATG	9 cut(s)	61, 350, 665, 677, 734, 1021, 1067, 1106, 1598
FaqI	GGGAC	1 cut(s)	2046
FatI	CATG	9 cut(s)	57, 346, 661, 673, 730, 1017, 1063, 1102, 1594
FauI	CCCGC	1 cut(s)	1664
FauNDI	CATATG	1 cut(s)	1716
FbaI	TGATCA	1 cut(s)	889
FblI	GTMKAC	2 cut(s)	544, 590
Fnu4HI	GCNGC	6 cut(s)	351, 1304, 1416, 1614, 1749, 2121
FokI	GGATG	3 cut(s)	177, 1194, 1877
FriOI	GRGCYC	3 cut(s)	346, 943, 1479
Fsp4HI	GCNGC	6 cut(s)	351, 1304, 1416, 1614, 1749, 2121
FspBI	CTAG	5 cut(s)	21, 558, 792, 1433, 1871
GlaI	GCGC	3 cut(s)	1120, 1428, 1686
GluI	GCNGC	6 cut(s)	351, 1304, 1416, 1614, 1749, 2121
GsuI	CTGGAG	1 cut(s)	2055
HaeIII	GGCC	2 cut(s)	824, 838
HapII	CCGG	5 cut(s)	289, 1261, 2327, 2436, 2450
HgaI	GACGC	2 cut(s)	1266, 1364
HhaI	GCGC	3 cut(s)	1121, 1429, 1687
Hin1I	GRCGYC	1 cut(s)	1258
Hin1II	CATG	9 cut(s)	61, 350, 665, 677, 734, 1021, 1067, 1106, 1598
Hin6I	GCGC	3 cut(s)	1119, 1427, 1685
HinP1I	GCGC	3 cut(s)	1119, 1427, 1685
HindIII	AAGCTT	5 cut(s)	425, 647, 764, 1008, 1505
HpaII	CCGG	5 cut(s)	289, 1261, 2327, 2436, 2450
HphI	GGTGA	5 cut(s)	162, 511, 1033, 1541, 2210
Hpy166II	GTNNAC	7 cut(s)	545, 591, 1496, 1967, 2097, 2312, 2441
Hpy188III	TCNNGA	9 cut(s)	431, 1036, 1163, 1229, 1254, 1517, 1729, 1809, 2021
Hpy8I	GTNNAC	7 cut(s)	545, 591, 1496, 1967, 2097, 2312, 2441
Hpy99I	CGWCG	2 cut(s)	450, 2249
HpyAV	CCTTC	6 cut(s)	361, 449, 1020, 1520, 2189, 2326
HpyCH4IV	ACGT	3 cut(s)	1760, 1830, 2244
HpyCH4V	TGCA	8 cut(s)	139, 661, 871, 964, 1303, 1461, 1635, 2036
HpySE526I	ACGT	3 cut(s)	1760, 1830, 2244
Hsp92I	GRCGYC	1 cut(s)	1258
Hsp92II	CATG	9 cut(s)	61, 350, 665, 677, 734, 1021, 1067, 1106, 1598
HspAI	GCGC	3 cut(s)	1119, 1427, 1685
Ksp22I	TGATCA	1 cut(s)	889
LmnI	GCTCC	6 cut(s)	433, 529, 628, 1055, 1116, 1369
Lsp1109I	GCAGC	3 cut(s)	337, 1315, 1427
LweI	GCATC	6 cut(s)	303, 1290, 1438, 1448, 1622, 2461
MabI	ACCWGGT	1 cut(s)	1464
MaeI	CTAG	5 cut(s)	21, 558, 792, 1433, 1871
MaeII	ACGT	3 cut(s)	1760, 1830, 2244
MflI	RGATCY	3 cut(s)	800, 1225, 2111
MhlI	GDGCHC	3 cut(s)	346, 943, 1479
MlsI	TGGCCA	1 cut(s)	838
MluCI	AATT	6 cut(s)	523, 780, 862, 1219, 1909, 2065
MluNI	TGGCCA	1 cut(s)	838
MlyI	GAGTC	5 cut(s)	90, 1491, 1585, 2026, 2416
MmeI	TCCRAC	2 cut(s)	778, 1613
Mox20I	TGGCCA	1 cut(s)	838
Mph1103I	ATGCAT	1 cut(s)	663
MroXI	GAANNNNTTC	1 cut(s)	1984
MscI	TGGCCA	1 cut(s)	838
MseI	TTAA	9 cut(s)	156, 417, 1083, 1308, 1710, 1913, 1973, 2240, 2250
MslI	CAYNNNNRTG	2 cut(s)	260, 735
Msp20I	TGGCCA	1 cut(s)	838
MspI	CCGG	5 cut(s)	289, 1261, 2327, 2436, 2450
MspR9I	CCNGG	3 cut(s)	1146, 1466, 2085
Mva1269I	GAATGC	2 cut(s)	871, 2274
MvaI	CCWGG	3 cut(s)	1146, 1466, 2085
MvnI	CGCG	1 cut(s)	1427
NdeI	CATATG	1 cut(s)	1716
NlaIII	CATG	9 cut(s)	61, 350, 665, 677, 734, 1021, 1067, 1106, 1598
NlaIV	GGNNCC	5 cut(s)	200, 555, 802, 1227, 2285
NmuCI	GTSAC	3 cut(s)	102, 1123, 1873
NsiI	ATGCAT	1 cut(s)	663
NspI	RCATGY	1 cut(s)	677
OliI	CACNNNNGTG	1 cut(s)	260
PaeI	GCATGC	1 cut(s)	677
PaeR7I	CTCGAG	1 cut(s)	2404
PcsI	WCGNNNNNNNCGW	1 cut(s)	1041
PctI	GAATGC	2 cut(s)	871, 2274
PdmI	GAANNNNTTC	1 cut(s)	1984
PflMI	CCANNNNNTGG	1 cut(s)	2279
PinAI	ACCGGT	1 cut(s)	2449
PkrI	GCNGC	6 cut(s)	352, 1305, 1417, 1615, 1750, 2122
PleI	GAGTC	5 cut(s)	89, 1491, 1585, 2025, 2415
PpsI	GAGTC	5 cut(s)	89, 1491, 1585, 2025, 2415
PshAI	GACNNNNGTC	1 cut(s)	1595
Psp124BI	GAGCTC	1 cut(s)	1479
Psp6I	CCWGG	3 cut(s)	1144, 1464, 2083
PspGI	CCWGG	3 cut(s)	1144, 1464, 2083
PspN4I	GGNNCC	5 cut(s)	200, 555, 802, 1227, 2285
PspPI	GGNCC	2 cut(s)	44, 1967
PspXI	VCTCGAGB	1 cut(s)	2404
PstNI	CAGNNNCTG	3 cut(s)	1127, 1988, 2192
PsuI	RGATCY	3 cut(s)	800, 1225, 2111
RsaI	GTAC	4 cut(s)	575, 1200, 1568, 2313
RsaNI	GTAC	4 cut(s)	574, 1199, 1567, 2312
RseI	CAYNNNNRTG	2 cut(s)	260, 735
SacI	GAGCTC	1 cut(s)	1479
SaqAI	TTAA	9 cut(s)	156, 417, 1083, 1308, 1710, 1913, 1973, 2240, 2250
SatI	GCNGC	6 cut(s)	351, 1304, 1416, 1614, 1749, 2121
Sau96I	GGNCC	2 cut(s)	44, 1967
ScaI	AGTACT	1 cut(s)	1568
SchI	GAGTC	5 cut(s)	90, 1491, 1585, 2026, 2416
ScrFI	CCNGG	3 cut(s)	1146, 1466, 2085
SduI	GDGCHC	3 cut(s)	346, 943, 1479
SexAI	ACCWGGT	1 cut(s)	1464
SfaNI	GCATC	6 cut(s)	303, 1290, 1438, 1448, 1622, 2461
SfcI	CTRYAG	4 cut(s)	91, 845, 1698, 2207
Sfr274I	CTCGAG	1 cut(s)	2404
SgrAI	CRCCGGYG	1 cut(s)	1260
SinI	GGWCC	2 cut(s)	44, 1967
SlaI	CTCGAG	1 cut(s)	2404
SmiMI	CAYNNNNRTG	2 cut(s)	260, 735
SmlI	CTYRAG	5 cut(s)	204, 826, 1161, 1727, 2404
SmoI	CTYRAG	5 cut(s)	204, 826, 1161, 1727, 2404
SphI	GCATGC	1 cut(s)	677
Sse9I	AATT	6 cut(s)	523, 780, 862, 1219, 1909, 2065
SsiI	CCGC	9 cut(s)	218, 275, 316, 1613, 1671, 1720, 1739, 1749, 2120
SspMI	CTAG	5 cut(s)	21, 558, 792, 1433, 1871
SstI	GAGCTC	1 cut(s)	1479
StyD4I	CCNGG	3 cut(s)	1144, 1464, 2083
StyI	CCWWGG	1 cut(s)	1621
TaiI	ACGT	3 cut(s)	1763, 1833, 2247
TasI	AATT	6 cut(s)	523, 780, 862, 1219, 1909, 2065
TatI	WGTACW	3 cut(s)	573, 1566, 2311
TauI	GCSGC	3 cut(s)	1616, 1751, 2123
Tru1I	TTAA	9 cut(s)	156, 417, 1083, 1308, 1710, 1913, 1973, 2240, 2250
Tru9I	TTAA	9 cut(s)	156, 417, 1083, 1308, 1710, 1913, 1973, 2240, 2250
TscAI	CASTG	5 cut(s)	1128, 1759, 1866, 1882, 1927
TseFI	GTSAC	3 cut(s)	102, 1123, 1873
TseI	GCWGC	3 cut(s)	350, 1303, 1415
Tsp45I	GTSAC	3 cut(s)	102, 1123, 1873
TspGWI	ACGGA	2 cut(s)	1791, 2244
TspRI	CASTG	5 cut(s)	1128, 1759, 1866, 1882, 1927
Van91I	CCANNNNNTGG	1 cut(s)	2279
VpaK11BI	GGWCC	2 cut(s)	44, 1967
XapI	RAATTY	2 cut(s)	862, 1909
XceI	RCATGY	1 cut(s)	677
XhoI	CTCGAG	1 cut(s)	2404
XmiI	GTMKAC	2 cut(s)	544, 590
XmnI	GAANNNNTTC	1 cut(s)	1984
XspI	CTAG	5 cut(s)	21, 558, 792, 1433, 1871
ZrmI	AGTACT	1 cut(s)	1568
Zsp2I	ATGCAT	1 cut(s)	663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.