Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0007372

Physical Location & Seq

Genomic Coordinates Forward (+)

7236717 .. 7239032

Gene Structure 2316 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g07220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGGCAAAGGATAGAAGTGGAGAGATTGTTCCGTATCATCCTCCTTTTAATTTGGGTCCAGAGGAAGGGTCGATGCCAGTGCGTAGGTGGAGGGATGGTAGCTTGGAGATAGATTTACCTATGATGCAACTGAAAGAACTGTTCAACGTTAATCATTTTATTGTCAGTCAGGCAAATCCTCATATTGCACCATTGTTAAGGCTAAAGGAATTTGTGAGAGCTTGTGGAGGCAACTTTGCTGCTAAGCTTGCTCATCTTGCTGAAATGGAGGTGAAGCATAGATGTAATCAGATCCTGGAACTTGGTTTTCCATTAGGTGGACTTGCCAAGCTGTTCGCTCAAGATTGGGAGGGTGACGTTACTGTTGTTATGCCGGCTACACTTGCTCAGTACTCTAAAATTATACAAAACCCAACACATATTGAGCCTCAAAAGGCAGCCAACCAAGGTAGAAGGTGCACTTGGGAGAAGCTCTCGGCCATAAAAGCAAACTGTGGCATTGAGCTTGCTCTTGATGAGTGTGTTGTAATTCTCAACCACATGCGTAGACTCAAAAGGAGTGCAGAAAGAGCTGCGGCTTCTTCTTCTTCTCATGGCCTAGCTAGTACAAACAAATTTAGTGCATCTAGAAGAATTCCGTCTTGGAATTGCATTGCTCGAGAGAATTCAAGTGGTTCCCTTGAAGAAGACCTCATTGGAGATGGTGGTTCCTCATTCCATCATGGTGTTGGCGCATCTACCAGTGGAGTTCATTCTGGTAAAAGCTTTCAAACCCACCATAATATACATGATGGAAGTGACAGTCAATCCGAGAACGTTGATTTTAATTCTTGGACGAGATCTGGTGGACCCTTGATGAGGACAACATCAGCAAATAAGCTCATCGACTTTGTCCAAGATTTGGACATTGATGCTGAATTGAACAGAAGTTTGCTGACTAATCCTAACTCAATGACGCTTCAGATGGGAGGCAATAATCATTATTATCAAAGCCCGAGAGGTACAACACCTGAAAGAAGTCCCGAAAGCATAAAATTTGATAAGAGGGAGTTTGGAAGCATGGTTTCAGCAAATGGTTTTAGCATCACGGTGACTGAAGGTGATCTGTTGCAGCCTGAAAGGATACCTAATGGGATTGTATTTAATGTTGTGAAGAAAGAAACATTGTCAAGTAGGAGTAGTCATGACGGGGAAAATTACGGGAGTGAAGAAGCTGAATGTGTGCAACTTGACAGTCTGGAAAATGACATGGACGCTAGGTCTGCATCTGAAGATGATGATGCCGATATCACTTCCGCAGCCTTCTTAAATGAAACGGAACCCAATTGCCTGTCAACGGATCAACCTAAGGAAGAAGATTGTAATCATCAGAGCACTGTGGATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005811 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0012511 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019433 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044464 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0052689 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00100 ko00561 ko00564 ko00565 ko00590 ko00591 ko00592 ko01100 ko01110 map00100 map00561 map00564 map00565 map00590 map00591 map00592 map01100 map01110

Pfam Domains

Protein Families

DUF3336 Patatin

Protein Analysis

463

Amino Acids

50.77

Weight (kDa)

5.3

Isoelectric Point (pI)

56.72

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007372)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04040 AT5G04040
fragaria_vesca	FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040
malus_domestica	MD12G1087400.v1.1 MD14G1083100.v1.1
prunus_persica	Prupe.7G047400_v2.0.a1
pyrus_communis	pycom12g07540 pycom14g07220
rosa_chinensis	RchiOBHm_Chr2g0124811
rosa_laevigata	RLG00000018823
rosa_multiflora	Rmu_sc0002939.1_g000008
rosa_roxburghii	Rroxscaffold_2G00119660
rosa_rugosa	Rorug02G0252600 Rorug02G0252700 Rorug02G0252800
rosa_samantha	Rh2AG312300 Rh2BG320500 Rh2CG300500 Rh2DG337700
rosa_wichuraiana	Rw2G025220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	317, 901
AccI	GTMKAC	1 cut(s)	547
AciI	CCGC	2 cut(s)	575, 1296
AclI	AACGTT	2 cut(s)	147, 816
AclWI	GGATC	2 cut(s)	286, 1347
AcoI	YGGCCR	1 cut(s)	477
AcsI	RAATTY	5 cut(s)	209, 614, 633, 664, 1034
AcuI	CTGAAG	3 cut(s)	944, 1116, 1290
AfaI	GTAC	3 cut(s)	392, 607, 1003
AfiI	CCNNNNNNNGG	5 cut(s)	65, 317, 858, 901, 1336
AgsI	TTSAA	5 cut(s)	145, 669, 683, 770, 922
AhdI	GACNNNNNGTC	1 cut(s)	1258
AjnI	CCWGG	1 cut(s)	294
AjuI	GAANNNNNNNTTGG	6 cut(s)	445, 477, 625, 657, 678, 710
AloI	GAACNNNNNNTCC	2 cut(s)	12, 44
Alw21I	GWGCWC	2 cut(s)	461, 1376
Alw44I	GTGCAC	1 cut(s)	457
AlwI	GGATC	2 cut(s)	286, 1347
AlwNI	CAGNNNCTG	1 cut(s)	295
Ama87I	CYCGRG	2 cut(s)	657, 994
AoxI	GGCC	2 cut(s)	477, 595
ApaLI	GTGCAC	1 cut(s)	457
ApeKI	GCWGC	5 cut(s)	239, 437, 572, 1111, 1298
ApoI	RAATTY	5 cut(s)	209, 614, 633, 664, 1034
AspLEI	GCGC	1 cut(s)	734
AspS9I	GGNCC	2 cut(s)	56, 848
AsuHPI	GGTGA	4 cut(s)	283, 365, 1102, 1112
AvaI	CYCGRG	2 cut(s)	657, 994
AvaII	GGWCC	2 cut(s)	56, 848
AxyI	CCTNAGG	1 cut(s)	1347
BaeGI	GKGCMC	1 cut(s)	461
BbsI	GAAGAC	1 cut(s)	693
Bbv12I	GWGCWC	2 cut(s)	461, 1376
BbvI	GCAGC	5 cut(s)	226, 449, 559, 1123, 1310
BccI	CCATC	6 cut(s)	89, 695, 726, 785, 958, 1376
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	1 cut(s)	296
BciVI	GTATCC	1 cut(s)	1116
BfaI	CTAG	4 cut(s)	599, 603, 627, 1257
BfuI	GTATCC	1 cut(s)	1116
BglII	AGATCT	1 cut(s)	839
BisI	GCNGC	6 cut(s)	240, 438, 573, 576, 1112, 1299
BlpI	GCTNAGC	1 cut(s)	243
BlsI	GCNGC	6 cut(s)	241, 439, 574, 577, 1113, 1300
BmcAI	AGTACT	1 cut(s)	392
Bme1390I	CCNGG	1 cut(s)	296
Bme18I	GGWCC	2 cut(s)	56, 848
BmeRI	GACNNNNNGTC	1 cut(s)	1258
BmeT110I	CYCGRG	2 cut(s)	657, 994
BmgT120I	GGNCC	2 cut(s)	56, 848
BmiI	GGNNCC	5 cut(s)	57, 676, 709, 850, 1320
BmrFI	CCNGG	1 cut(s)	296
BmsI	GCATC	8 cut(s)	63, 114, 632, 743, 901, 1092, 1270, 1274
BpiI	GAAGAC	1 cut(s)	693
BplI	GAGNNNNNCTC	2 cut(s)	458, 490
Bpu1102I	GCTNAGC	1 cut(s)	243
BpuEI	CTTGAG	1 cut(s)	324
BsaBI	GATNNNNATC	1 cut(s)	1362
BsaJI	CCNNGG	1 cut(s)	445
BsaXI	ACNNNNNCTCC	4 cut(s)	12, 42, 82, 112
Bsc4I	CCNNNNNNNGG	5 cut(s)	65, 317, 858, 901, 1336
Bse118I	RCCGGY	1 cut(s)	373
Bse1I	ACTGG	2 cut(s)	77, 741
Bse21I	CCTNAGG	1 cut(s)	1347
Bse3DI	GCAATG	1 cut(s)	651
Bse8I	GATNNNNATC	1 cut(s)	1362
BseBI	CCWGG	1 cut(s)	296
BseDI	CCNNGG	1 cut(s)	445
BseGI	GGATG	3 cut(s)	37, 100, 1387
BseJI	GATNNNNATC	1 cut(s)	1362
BseLI	CCNNNNNNNGG	5 cut(s)	65, 317, 858, 901, 1336
BseMI	GCAATG	1 cut(s)	651
BseMII	CTCAG	1 cut(s)	401
BseNI	ACTGG	2 cut(s)	77, 741
BseSI	GKGCMC	1 cut(s)	461
BseXI	GCAGC	5 cut(s)	226, 449, 559, 1123, 1310
BsgI	GTGCAG	1 cut(s)	582
BshFI	GGCC	2 cut(s)	479, 597
BsiHKAI	GWGCWC	2 cut(s)	461, 1376
BsiHKCI	CYCGRG	2 cut(s)	657, 994
BsiSI	CCGG	1 cut(s)	374
BslFI	GGGAC	1 cut(s)	1005
BslI	CCNNNNNNNGG	5 cut(s)	65, 317, 858, 901, 1336
BsmFI	GGGAC	1 cut(s)	1005
BsnI	GGCC	2 cut(s)	479, 597
BsoBI	CYCGRG	2 cut(s)	657, 994
Bsp1286I	GDGCHC	2 cut(s)	461, 1376
Bsp143I	GATC	4 cut(s)	291, 839, 1102, 1339
Bsp1720I	GCTNAGC	1 cut(s)	243
BspACI	CCGC	2 cut(s)	575, 1296
BspANI	GGCC	2 cut(s)	479, 597
BspCNI	CTCAG	1 cut(s)	400
BspHI	TCATGA	1 cut(s)	1183
BspLI	GGNNCC	5 cut(s)	57, 676, 709, 850, 1320
BspPI	GGATC	2 cut(s)	286, 1347
BsrDI	GCAATG	1 cut(s)	651
BsrFI	RCCGGY	1 cut(s)	373
BsrI	ACTGG	2 cut(s)	77, 741
BssAI	RCCGGY	1 cut(s)	373
BssECI	CCNNGG	1 cut(s)	445
BssMI	GATC	4 cut(s)	291, 839, 1102, 1339
BssT1I	CCWWGG	1 cut(s)	445
Bst2UI	CCWGG	1 cut(s)	296
Bst4CI	ACNGT	7 cut(s)	141, 364, 494, 803, 1090, 1235, 1378
BstC8I	GCNNGC	3 cut(s)	249, 375, 507
BstDEI	CTNAG	3 cut(s)	243, 387, 1347
BstENI	CCTNNNNNAGG	1 cut(s)	856
BstF5I	GGATG	3 cut(s)	37, 100, 1387
BstHHI	GCGC	1 cut(s)	734
BstKTI	GATC	4 cut(s)	294, 842, 1105, 1342
BstMBI	GATC	4 cut(s)	291, 839, 1102, 1339
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 257, 383, 485, 569, 1262
BstNI	CCWGG	1 cut(s)	296
BstNSI	RCATGY	1 cut(s)	544
BstSCI	CCNGG	1 cut(s)	294
BstSLI	GKGCMC	1 cut(s)	461
BstV1I	GCAGC	5 cut(s)	226, 449, 559, 1123, 1310
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	2 cut(s)	291, 839
BstYI	RGATCY	2 cut(s)	291, 839
Bsu36I	CCTNAGG	1 cut(s)	1347
BsuI	GTATCC	1 cut(s)	1116
BsuRI	GGCC	2 cut(s)	479, 597
BtsCI	GGATG	3 cut(s)	37, 100, 1387
BtsIMutI	CAGTG	3 cut(s)	84, 748, 1374
Cac8I	GCNNGC	3 cut(s)	249, 375, 507
CaiI	CAGNNNCTG	1 cut(s)	295
CciI	TCATGA	1 cut(s)	1183
CfoI	GCGC	1 cut(s)	734
Cfr10I	RCCGGY	1 cut(s)	373
Cfr13I	GGNCC	2 cut(s)	56, 848
CseI	GACGC	2 cut(s)	964, 1262
Csp6I	GTAC	3 cut(s)	391, 606, 1002
CviAII	CATG	7 cut(s)	541, 593, 722, 788, 1060, 1184, 1249
CviQI	GTAC	3 cut(s)	391, 606, 1002
DdeI	CTNAG	3 cut(s)	243, 387, 1347
DpnI	GATC	4 cut(s)	293, 841, 1104, 1341
DpnII	GATC	4 cut(s)	291, 839, 1102, 1339
DriI	GACNNNNNGTC	1 cut(s)	1258
EaeI	YGGCCR	1 cut(s)	477
Eam1105I	GACNNNNNGTC	1 cut(s)	1258
Eco130I	CCWWGG	1 cut(s)	445
Eco32I	GATATC	1 cut(s)	1288
Eco47I	GGWCC	2 cut(s)	56, 848
Eco57I	CTGAAG	3 cut(s)	944, 1116, 1290
Eco81I	CCTNAGG	1 cut(s)	1347
Eco88I	CYCGRG	2 cut(s)	657, 994
EcoNI	CCTNNNNNAGG	1 cut(s)	856
EcoRI	GAATTC	2 cut(s)	633, 664
EcoRII	CCWGG	1 cut(s)	294
EcoRV	GATATC	1 cut(s)	1288
EcoT14I	CCWWGG	1 cut(s)	445
ErhI	CCWWGG	1 cut(s)	445
FaeI	CATG	7 cut(s)	544, 596, 725, 791, 1063, 1187, 1252
FalI	AAGNNNNNCTT	2 cut(s)	445, 477
FaqI	GGGAC	1 cut(s)	1005
FatI	CATG	7 cut(s)	540, 592, 721, 787, 1059, 1183, 1248
FblI	GTMKAC	1 cut(s)	547
Fnu4HI	GCNGC	6 cut(s)	240, 438, 573, 576, 1112, 1299
FokI	GGATG	2 cut(s)	24, 107
Fsp4HI	GCNGC	6 cut(s)	240, 438, 573, 576, 1112, 1299
FspBI	CTAG	4 cut(s)	599, 603, 627, 1257
GlaI	GCGC	1 cut(s)	733
GluI	GCNGC	6 cut(s)	240, 438, 573, 576, 1112, 1299
HaeIII	GGCC	2 cut(s)	479, 597
HapII	CCGG	1 cut(s)	374
HgaI	GACGC	2 cut(s)	964, 1262
HhaI	GCGC	1 cut(s)	734
Hin1II	CATG	7 cut(s)	544, 596, 725, 791, 1063, 1187, 1252
Hin6I	GCGC	1 cut(s)	732
HinP1I	GCGC	1 cut(s)	732
HincII	GTYRAC	1 cut(s)	1335
HindII	GTYRAC	1 cut(s)	1335
HindIII	AAGCTT	2 cut(s)	245, 763
HinfI	GANTC	1 cut(s)	549
HpaII	CCGG	1 cut(s)	374
HphI	GGTGA	4 cut(s)	283, 365, 1102, 1112
Hpy166II	GTNNAC	5 cut(s)	320, 459, 548, 848, 1335
Hpy188I	TCNGA	5 cut(s)	291, 811, 963, 1270, 1371
Hpy188III	TCNNGA	8 cut(s)	59, 341, 512, 627, 659, 1022, 1184, 1238
Hpy8I	GTNNAC	5 cut(s)	320, 459, 548, 848, 1335
HpyAV	CCTTC	4 cut(s)	59, 447, 1091, 1312
HpyCH4III	ACNGT	7 cut(s)	141, 364, 494, 803, 1090, 1235, 1378
HpyCH4IV	ACGT	3 cut(s)	147, 357, 816
HpyCH4V	TGCA	9 cut(s)	127, 188, 459, 563, 623, 651, 1111, 1225, 1265
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 257, 383, 485, 569, 1262
HpyF3I	CTNAG	3 cut(s)	243, 387, 1347
HpySE526I	ACGT	3 cut(s)	147, 357, 816
Hsp92II	CATG	7 cut(s)	544, 596, 725, 791, 1063, 1187, 1252
HspAI	GCGC	1 cut(s)	732
KroI	GCCGGC	1 cut(s)	373
KroNI	GCCGGC	1 cut(s)	375
Kzo9I	GATC	4 cut(s)	291, 839, 1102, 1339
Lsp1109I	GCAGC	5 cut(s)	226, 449, 559, 1123, 1310
LweI	GCATC	8 cut(s)	63, 114, 632, 743, 901, 1092, 1270, 1274
MaeI	CTAG	4 cut(s)	599, 603, 627, 1257
MaeII	ACGT	3 cut(s)	147, 357, 816
MaeIII	GTNAC	4 cut(s)	353, 358, 797, 1090
MalI	GATC	4 cut(s)	293, 841, 1104, 1341
MboI	GATC	4 cut(s)	291, 839, 1102, 1339
MfeI	CAATTG	1 cut(s)	1324
MflI	RGATCY	2 cut(s)	291, 839
MhlI	GDGCHC	2 cut(s)	461, 1376
MlyI	GAGTC	1 cut(s)	543
MroNI	GCCGGC	1 cut(s)	373
MseI	TTAA	6 cut(s)	48, 150, 197, 825, 1143, 1307
MslI	CAYNNNNRTG	2 cut(s)	723, 1088
MspI	CCGG	1 cut(s)	374
MspR9I	CCNGG	1 cut(s)	296
MunI	CAATTG	1 cut(s)	1324
MvaI	CCWGG	1 cut(s)	296
MwoI	GCNNNNNNNGC	6 cut(s)	248, 257, 383, 485, 569, 1262
NaeI	GCCGGC	1 cut(s)	375
NdeII	GATC	4 cut(s)	291, 839, 1102, 1339
NgoMIV	GCCGGC	1 cut(s)	373
NlaIII	CATG	7 cut(s)	544, 596, 725, 791, 1063, 1187, 1252
NlaIV	GGNNCC	5 cut(s)	57, 676, 709, 850, 1320
NmeAIII	GCCGAG	1 cut(s)	455
NmuCI	GTSAC	3 cut(s)	353, 797, 1090
NspI	RCATGY	1 cut(s)	544
PaeR7I	CTCGAG	1 cut(s)	657
PagI	TCATGA	1 cut(s)	1183
PdiI	GCCGGC	1 cut(s)	375
PflFI	GACNNNGTC	1 cut(s)	890
PflMI	CCANNNNNTGG	2 cut(s)	317, 901
PfoI	TCCNGGA	1 cut(s)	294
PkrI	GCNGC	6 cut(s)	241, 439, 574, 577, 1113, 1300
PleI	GAGTC	1 cut(s)	543
PpsI	GAGTC	1 cut(s)	543
Psp1406I	AACGTT	2 cut(s)	147, 816
Psp6I	CCWGG	1 cut(s)	294
PspGI	CCWGG	1 cut(s)	294
PspN4I	GGNNCC	5 cut(s)	57, 676, 709, 850, 1320
PspPI	GGNCC	2 cut(s)	56, 848
PstNI	CAGNNNCTG	1 cut(s)	295
PsuI	RGATCY	2 cut(s)	291, 839
PsyI	GACNNNGTC	1 cut(s)	890
RsaI	GTAC	3 cut(s)	392, 607, 1003
RsaNI	GTAC	3 cut(s)	391, 606, 1002
RseI	CAYNNNNRTG	2 cut(s)	723, 1088
SaqAI	TTAA	6 cut(s)	48, 150, 197, 825, 1143, 1307
SatI	GCNGC	6 cut(s)	240, 438, 573, 576, 1112, 1299
Sau3AI	GATC	4 cut(s)	291, 839, 1102, 1339
Sau96I	GGNCC	2 cut(s)	56, 848
ScaI	AGTACT	1 cut(s)	392
SchI	GAGTC	1 cut(s)	543
ScrFI	CCNGG	1 cut(s)	296
SduI	GDGCHC	2 cut(s)	461, 1376
SfaNI	GCATC	8 cut(s)	63, 114, 632, 743, 901, 1092, 1270, 1274
Sfr274I	CTCGAG	1 cut(s)	657
SinI	GGWCC	2 cut(s)	56, 848
SlaI	CTCGAG	1 cut(s)	657
SmiMI	CAYNNNNRTG	2 cut(s)	723, 1088
SmlI	CTYRAG	2 cut(s)	339, 657
SmoI	CTYRAG	2 cut(s)	339, 657
SsiI	CCGC	2 cut(s)	575, 1296
SspMI	CTAG	4 cut(s)	599, 603, 627, 1257
StyD4I	CCNGG	1 cut(s)	294
StyI	CCWWGG	1 cut(s)	445
TaaI	ACNGT	7 cut(s)	141, 364, 494, 803, 1090, 1235, 1378
TaiI	ACGT	3 cut(s)	150, 360, 819
TaqI	TCGA	3 cut(s)	71, 658, 885
TatI	WGTACW	2 cut(s)	390, 605
TauI	GCSGC	1 cut(s)	578
Tru1I	TTAA	6 cut(s)	48, 150, 197, 825, 1143, 1307
Tru9I	TTAA	6 cut(s)	48, 150, 197, 825, 1143, 1307
TscAI	CASTG	3 cut(s)	84, 748, 1381
TseFI	GTSAC	3 cut(s)	353, 797, 1090
TseI	GCWGC	5 cut(s)	239, 437, 572, 1111, 1298
Tsp45I	GTSAC	3 cut(s)	353, 797, 1090
TspDTI	ATGAA	2 cut(s)	740, 1326
TspGWI	ACGGA	4 cut(s)	21, 627, 1331, 1352
TspRI	CASTG	3 cut(s)	84, 748, 1381
Tth111I	GACNNNGTC	1 cut(s)	890
Van91I	CCANNNNNTGG	2 cut(s)	317, 901
VneI	GTGCAC	1 cut(s)	457
VpaK11BI	GGWCC	2 cut(s)	56, 848
XagI	CCTNNNNNAGG	1 cut(s)	856
XapI	RAATTY	5 cut(s)	209, 614, 633, 664, 1034
XbaI	TCTAGA	1 cut(s)	626
XceI	RCATGY	1 cut(s)	544
XcmI	CCANNNNNNNNNTGG	2 cut(s)	84, 725
XhoI	CTCGAG	1 cut(s)	657
XmiI	GTMKAC	1 cut(s)	547
XspI	CTAG	4 cut(s)	599, 603, 627, 1257
ZrmI	AGTACT	1 cut(s)	392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.