Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007372

Physical Location & Seq

Genomic Coordinates Reverse (-)

10749661 .. 10754401

Gene Structure 4741 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1087400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2535 bp

ATGGATATAAGTAATGAGGCCAGTGTTGATCCGTTTCCAATTGGACCTTCAACCATTGTTGGCCGGACAATCGCTTTCAGGGTTTTATTTTGCAAGTCAATGTCACACTTGAGGCATCAAATATTTCGTACGTTTTTGAGTTTTGTCTATAGATTCAAAAACTTTTTGGCTCCCATGTTCTCATGGTTGCATCCTCGAAACCCCCAAGGGATATTGGCAATGGTGACAATCATTGCTTTTTTGTTGAAACGTTACACGAATGTCAAAGTCAAGGCAGAAATGGCTTATCGGAGGAAATTTTGGAGAAATATGATGAGAACTGCTTTGACCTATGAGGAGTGGGCTCATGCCGCTAGGATGCTTGATAAAGAGACACCAAAGATGAATGAATCAGACCTTTATGATCAAGAAGTAGTTAGGAACAAGCTTCAAGAGCTCCATCACCGTCGTGAAGAGGGTTCGCTCAGAGATATTATGTTCTGCATGCGAGCAGATCTTGTTAGAAATCTCGGTAACATGTGCAACCCTGAGCTTCACAAGGAGAAACTTCATGTTCCTAAACTCATCAAGGAATACATTGATGAGGTCACAACTCAGTTGAGAATGGTTTGTGATTCGGACTCAGAAGAGCTCTCGTTGGAAGAGAAGCTGGCTTTCATGCATGAAACAAGGCATGCTTTTGGGAGAACTGCCTTGCTGTTGAGTGGGGGTGCTTCTCTTGGATCTTTCCATGTCGGTGTGGTTAAAACACTGGTCGAACATAAGCTTTTGCCAAGAATAATCGCTGGTTCCAGTGTAGGATCCATTATGTGTTCTGTTGTTGCCACTAGGTCTTGGCCTGAGCTCCAGAGCTTTTTTGAGGATTCTTGGCACTCATTGCAGTTTTTTGATCAAATGGGCGGGATTTTTACAGTTGTCAAGAGGGTCATGACACGTGGGGCAGTTCATGAGATCAGGCAGTTGCAAATGATGTTAAGGCATCTAACGAGTAACCTGACATTTCAAGAAGCTTATGACATGACAGGTCGAATTCTTGGGATAACTGTTTGCTCCCCAAGGAAGCATGAGCCACCTAGATGCCTCAACTACTTGACTTCACCTCATGTTGTTATATGGAGTGCAGTGACAGCTTCTTGTGCTTTTCCAGGCCTTTTTGAGGCTCAGGAACTAATGGCAAAGGATAGAAGTGGAGAGATTGTTCCATATCATCCTCCATTTAATTTGGGTCCAGAGGAGGGGTCGATGCCAGTGCGTAGGTGGAGAGATGGTAGCTTGGAGATAGATTTACCTATGATGCAGCTGAAAGAACTGTTCAATGTTAATCATTTTATCGTCAGTCAGGCAAATCCTCATATTGCACCATTGTTACGGCTAAAGGAATTTGTGAGAGCTTGTGGAGGCAACTTCGCTGCTAAGCTTGCTCATCTTGCTGAAATGGAGGTGAAGCATAGATGTAACCAGATCCTGGAACTTGGTTTTCCATTAGGTGGAATTGCCAAGCTGTTCGCTCAAGATTGGGAGGGTGATGTTACTGTTGTTATGCCAGCCACACTTGCTCAGTACTCAAAAATCATACAAAATCCAACATATATTGAGCTTCAAAAGGCAGCCAACCAAGGTAGAAGGTGCACTTGGGAGAAGCTCTCGGCCATAAAAGCAAATTGTGGCATTGAGCTTGCTCTTGATGAGTGTGTTGTAATTCTCAACCACATGCGTAGACTCAAAAGGAGTGCAGAAAGAGCTGCGGCTTCTTCTCATGGCCTAGCTAGTACAACCAAATTCAGTGCATCTAGGAGAATTCCATCTTGGAATTGCATTGCTCGAGAAAATTCAAGTGGTTCCCTTGAAGAAGACCTCATTGGAGATGGTGGTTCCTCATTCCATCATGGTGTTGGCACATCTACTAGTGGAGTTCATTCTGGTAAAAGTTTTCAAACCCACCGTAATTTACATGATGGAAGTGACAGTGACTCTGAGAGTGTTGATTTCAATTCTTGGACAAGATCTGGTGGACCCTTGATGAGGACAACGTCAGCAAATAAGTTCATCGACTTTGTTCAAAATTTGGACATTGATGCTGAATTGAACAGAAGTGTGCTGACAAGTCCGAAGTCAATGATGGTTCAGATGGGAGGCAATAACCATTATTATCAAAGCTCAAGAGGTACAACACCTGAAAGAAGTCCTGAAAGCATAAAATTTGACAAGAGGGAGTTTGGAAGCATGGTTTCAGGAAACGGTTCTAGCATTACGGTGACTGAAGGTGATCTCTTGCAGCCTGAAAGGATACATAATGGAATTGTATTCAATATTGTGAAGAAAGAAACATTGTCAAGTGGGAGTAGTCATGACTTGGAAAATTACGGCAGTGAAGTAGTTGAATGTGTGCAACTTGACAGTCCGGAAAAGGACATGGATGCTGGGTCTGTATCTGAATATGGTGATGCCGATATCACTTCAGAAGCCTTCTTAAATGAAACGGAACCTAATTGCCAGTCAGTGGATCAACCTAAAGTAGATGATTGTAATCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005811 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0012511 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019433 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044464 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0052689 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00100 ko00561 ko00564 ko00565 ko00590 ko00591 ko00592 ko01100 ko01110 map00100 map00561 map00564 map00565 map00590 map00591 map00592 map01100 map01110

Pfam Domains

Protein Families

DUF3336 Patatin

Protein Analysis

845

Amino Acids

94.91

Weight (kDa)

6.55

Isoelectric Point (pI)

47.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3336	PF11815	94 - 225	2.9e-32	Domain of unknown function (DUF3336)
Patatin	PF01734	232 - 404	2.1e-18	Patatin-like phospholipase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007372)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04040 AT5G04040
fragaria_vesca	FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040
malus_domestica	MD12G1087400.v1.1 MD14G1083100.v1.1
prunus_persica	Prupe.7G047400_v2.0.a1
pyrus_communis	pycom12g07540 pycom14g07220
rosa_chinensis	RchiOBHm_Chr2g0124811
rosa_laevigata	RLG00000018823
rosa_multiflora	Rmu_sc0002939.1_g000008
rosa_roxburghii	Rroxscaffold_2G00119660
rosa_rugosa	Rorug02G0252600 Rorug02G0252700 Rorug02G0252800
rosa_samantha	Rh2AG312300 Rh2BG320500 Rh2CG300500 Rh2DG337700
rosa_wichuraiana	Rw2G025220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	3 cut(s)	1465, 1487, 2500
AccI	GTMKAC	1 cut(s)	1717
AccIII	TCCGGA	1 cut(s)	2401
AciI	CCGC	3 cut(s)	351, 900, 1745
AclI	AACGTT	1 cut(s)	250
AclWI	GGATC	6 cut(s)	23, 730, 795, 808, 1456, 2511
AcoI	YGGCCR	2 cut(s)	61, 1647
AcsI	RAATTY	8 cut(s)	296, 1029, 1379, 1778, 1797, 1828, 2062, 2198
AcuI	CTGAAG	2 cut(s)	2280, 2442
AcvI	CACGTG	1 cut(s)	935
AfaI	GTAC	4 cut(s)	130, 1562, 1771, 2167
AfiI	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1465, 1487, 2022, 2500
AflIII	ACRYGT	2 cut(s)	516, 932
AhlI	ACTAGT	1 cut(s)	1904
AjnI	CCWGG	2 cut(s)	1144, 1464
AjuI	GAANNNNNNNTTGG	8 cut(s)	31, 63, 1615, 1647, 1789, 1821, 1842, 1874
AleI	CACNNNNGTG	1 cut(s)	447
AloI	GAACNNNNNNTCC	2 cut(s)	1182, 1214
Alw21I	GWGCWC	4 cut(s)	438, 633, 846, 1631
Alw26I	GTCTC	1 cut(s)	365
Alw44I	GTGCAC	1 cut(s)	1627
AlwI	GGATC	6 cut(s)	23, 730, 795, 808, 1456, 2511
AlwNI	CAGNNNCTG	1 cut(s)	1465
Ama87I	CYCGRG	1 cut(s)	1821
Aor13HI	TCCGGA	1 cut(s)	2401
AoxI	GGCC	6 cut(s)	18, 61, 836, 1147, 1647, 1759
ApaLI	GTGCAC	1 cut(s)	1627
ApeKI	GCWGC	5 cut(s)	1297, 1409, 1607, 1742, 2275
ApoI	RAATTY	8 cut(s)	296, 1029, 1379, 1778, 1797, 1828, 2062, 2198
Asp700I	GAANNNNTTC	1 cut(s)	2239
AspS9I	GGNCC	3 cut(s)	44, 1226, 2012
AsuHPI	GGTGA	8 cut(s)	235, 434, 1089, 1453, 1535, 2266, 2276, 2453
AvaI	CYCGRG	1 cut(s)	1821
AvaII	GGWCC	3 cut(s)	44, 1226, 2012
BaeGI	GKGCMC	1 cut(s)	1631
BamHI	GGATCC	1 cut(s)	800
BanII	GRGCYC	4 cut(s)	346, 438, 633, 846
BbrPI	CACGTG	1 cut(s)	935
BbsI	GAAGAC	1 cut(s)	1857
Bbv12I	GWGCWC	4 cut(s)	438, 633, 846, 1631
BbvI	GCAGC	5 cut(s)	1309, 1396, 1619, 1729, 2287
BccI	CCATC	8 cut(s)	447, 1259, 1810, 1859, 1890, 1949, 2113, 2122
BceAI	ACGGC	2 cut(s)	1385, 2380
BcgI	CGANNNNNNTGC	2 cut(s)	1231, 1265
BciT130I	CCWGG	2 cut(s)	1146, 1466
BciVI	GTATCC	1 cut(s)	2280
BclI	TGATCA	2 cut(s)	403, 889
BcoDI	GTCTC	1 cut(s)	365
BcuI	ACTAGT	1 cut(s)	1904
BfaI	CTAG	8 cut(s)	354, 828, 1074, 1763, 1767, 1791, 1905, 2244
BfmI	CTRYAG	1 cut(s)	148
BfuI	GTATCC	1 cut(s)	2280
BglII	AGATCT	2 cut(s)	493, 2003
BisI	GCNGC	7 cut(s)	351, 1298, 1410, 1608, 1743, 1746, 2276
BlpI	GCTNAGC	1 cut(s)	1413
BlsI	GCNGC	7 cut(s)	352, 1299, 1411, 1609, 1744, 1747, 2277
BmcAI	AGTACT	1 cut(s)	1562
Bme1390I	CCNGG	2 cut(s)	1146, 1466
Bme18I	GGWCC	3 cut(s)	44, 1226, 2012
BmeT110I	CYCGRG	1 cut(s)	1821
BmgT120I	GGNCC	3 cut(s)	44, 1226, 2012
BmiI	GGNNCC	8 cut(s)	171, 790, 802, 1227, 1840, 1873, 2014, 2484
BmrFI	CCNGG	2 cut(s)	1146, 1466
BpiI	GAAGAC	1 cut(s)	1857
BplI	GAGNNNNNCTC	2 cut(s)	1628, 1660
BpmI	CTGGAG	1 cut(s)	830
Bpu10I	CCTNAGC	3 cut(s)	528, 840, 1161
Bpu1102I	GCTNAGC	1 cut(s)	1413
BpuEI	CTTGAG	3 cut(s)	130, 1494, 2143
BsaAI	YACGTR	1 cut(s)	935
BsaBI	GATNNNNATC	1 cut(s)	2526
BsaJI	CCNNGG	3 cut(s)	205, 1055, 1615
BsaWI	WCCGGW	1 cut(s)	2401
BsaXI	ACNNNNNCTCC	4 cut(s)	1182, 1212, 1252, 1282
Bsc4I	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1465, 1487, 2022, 2500
Bse1I	ACTGG	5 cut(s)	21, 756, 792, 1247, 2494
Bse3DI	GCAATG	4 cut(s)	225, 231, 875, 1815
Bse8I	GATNNNNATC	1 cut(s)	2526
BseAI	TCCGGA	1 cut(s)	2401
BseBI	CCWGG	2 cut(s)	1146, 1466
BseDI	CCNNGG	3 cut(s)	205, 1055, 1615
BseGI	GGATG	4 cut(s)	190, 363, 1207, 2422
BseJI	GATNNNNATC	1 cut(s)	2526
BseLI	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1465, 1487, 2022, 2500
BseMI	GCAATG	4 cut(s)	225, 231, 875, 1815
BseMII	CTCAG	8 cut(s)	478, 519, 608, 636, 831, 1175, 1571, 1965
BseNI	ACTGG	5 cut(s)	21, 756, 792, 1247, 2494
BseRI	GAGGAG	2 cut(s)	350, 1247
BseSI	GKGCMC	1 cut(s)	1631
BseXI	GCAGC	5 cut(s)	1309, 1396, 1619, 1729, 2287
BseYI	CCCAGC	1 cut(s)	2420
BsgI	GTGCAG	2 cut(s)	1140, 1752
BshFI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1761
BsiHKAI	GWGCWC	4 cut(s)	438, 633, 846, 1631
BsiHKCI	CYCGRG	1 cut(s)	1821
BsiSI	CCGG	2 cut(s)	64, 2402
BsiWI	CGTACG	1 cut(s)	128
BslI	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1465, 1487, 2022, 2500
BsmAI	GTCTC	1 cut(s)	365
BsnI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1761
BsoBI	CYCGRG	1 cut(s)	1821
Bsp1286I	GDGCHC	5 cut(s)	346, 438, 633, 846, 1631
Bsp13I	TCCGGA	1 cut(s)	2401
Bsp1720I	GCTNAGC	1 cut(s)	1413
BspACI	CCGC	3 cut(s)	351, 900, 1745
BspANI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1761
BspCNI	CTCAG	8 cut(s)	477, 520, 607, 635, 832, 1174, 1570, 1966
BspEI	TCCGGA	1 cut(s)	2401
BspHI	TCATGA	3 cut(s)	927, 946, 2347
BspLI	GGNNCC	8 cut(s)	171, 790, 802, 1227, 1840, 1873, 2014, 2484
BspPI	GGATC	6 cut(s)	23, 730, 795, 808, 1456, 2511
BspQI	GCTCTTC	1 cut(s)	621
BsrDI	GCAATG	4 cut(s)	225, 231, 875, 1815
BsrI	ACTGG	5 cut(s)	21, 756, 792, 1247, 2494
BssECI	CCNNGG	3 cut(s)	205, 1055, 1615
BssT1I	CCWWGG	3 cut(s)	205, 1055, 1615
Bst2UI	CCWGG	2 cut(s)	1146, 1466
Bst6I	CTCTTC	3 cut(s)	447, 621, 636
BstAPI	GCANNNNNTGC	1 cut(s)	877
BstBAI	YACGTR	1 cut(s)	935
BstC8I	GCNNGC	7 cut(s)	485, 489, 651, 675, 1419, 1545, 1677
BstDEI	CTNAG	9 cut(s)	464, 528, 594, 622, 840, 1161, 1413, 1557, 1974
BstENI	CCTNNNNNAGG	2 cut(s)	1154, 2020
BstF5I	GGATG	4 cut(s)	190, 363, 1207, 2422
BstMAI	GTCTC	1 cut(s)	365
BstNI	CCWGG	2 cut(s)	1146, 1466
BstNSI	RCATGY	4 cut(s)	487, 520, 677, 1714
BstSCI	CCNGG	2 cut(s)	1144, 1464
BstSFI	CTRYAG	1 cut(s)	148
BstSLI	GKGCMC	1 cut(s)	1631
BstV1I	GCAGC	5 cut(s)	1309, 1396, 1619, 1729, 2287
BstV2I	GAAGAC	1 cut(s)	1857
BstX2I	RGATCY	5 cut(s)	493, 722, 800, 1461, 2003
BstYI	RGATCY	5 cut(s)	493, 722, 800, 1461, 2003
BsuI	GTATCC	1 cut(s)	2280
BsuRI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1761
BtsCI	GGATG	4 cut(s)	190, 363, 1207, 2422
BtsI	GCAGTG	2 cut(s)	1128, 2374
BtsIMutI	CAGTG	9 cut(s)	28, 749, 799, 1128, 1254, 1789, 1972, 2374, 2505
Cac8I	GCNNGC	7 cut(s)	485, 489, 651, 675, 1419, 1545, 1677
CaiI	CAGNNNCTG	1 cut(s)	1465
CciI	TCATGA	3 cut(s)	927, 946, 2347
Cfr13I	GGNCC	3 cut(s)	44, 1226, 2012
Csp6I	GTAC	4 cut(s)	129, 1561, 1770, 2166
CviQI	GTAC	4 cut(s)	129, 1561, 1770, 2166
DdeI	CTNAG	9 cut(s)	464, 528, 594, 622, 840, 1161, 1413, 1557, 1974
EaeI	YGGCCR	2 cut(s)	61, 1647
Eam1104I	CTCTTC	3 cut(s)	447, 621, 636
EarI	CTCTTC	3 cut(s)	447, 621, 636
Ecl136II	GAGCTC	3 cut(s)	436, 631, 844
Eco130I	CCWWGG	3 cut(s)	205, 1055, 1615
Eco147I	AGGCCT	1 cut(s)	1149
Eco24I	GRGCYC	4 cut(s)	346, 438, 633, 846
Eco32I	GATATC	1 cut(s)	2452
Eco47I	GGWCC	3 cut(s)	44, 1226, 2012
Eco53kI	GAGCTC	3 cut(s)	436, 631, 844
Eco57I	CTGAAG	2 cut(s)	2280, 2442
Eco72I	CACGTG	1 cut(s)	935
Eco88I	CYCGRG	1 cut(s)	1821
EcoICRI	GAGCTC	3 cut(s)	436, 631, 844
EcoNI	CCTNNNNNAGG	2 cut(s)	1154, 2020
EcoRI	GAATTC	2 cut(s)	1029, 1797
EcoRII	CCWGG	2 cut(s)	1144, 1464
EcoRV	GATATC	1 cut(s)	2452
EcoT14I	CCWWGG	3 cut(s)	205, 1055, 1615
EcoT22I	ATGCAT	1 cut(s)	663
EcoT38I	GRGCYC	4 cut(s)	346, 438, 633, 846
ErhI	CCWWGG	3 cut(s)	205, 1055, 1615
FalI	AAGNNNNNCTT	4 cut(s)	661, 693, 1615, 1647
FauI	CCCGC	1 cut(s)	893
FbaI	TGATCA	2 cut(s)	403, 889
FblI	GTMKAC	1 cut(s)	1717
Fnu4HI	GCNGC	7 cut(s)	351, 1298, 1410, 1608, 1743, 1746, 2276
FokI	GGATG	4 cut(s)	177, 370, 1194, 2429
FriOI	GRGCYC	4 cut(s)	346, 438, 633, 846
Fsp4HI	GCNGC	7 cut(s)	351, 1298, 1410, 1608, 1743, 1746, 2276
FspBI	CTAG	8 cut(s)	354, 828, 1074, 1763, 1767, 1791, 1905, 2244
GluI	GCNGC	7 cut(s)	351, 1298, 1410, 1608, 1743, 1746, 2276
GsaI	CCCAGC	1 cut(s)	2424
GsuI	CTGGAG	1 cut(s)	830
HaeIII	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1761
HapII	CCGG	2 cut(s)	64, 2402
HindIII	AAGCTT	4 cut(s)	425, 764, 1008, 1415
HinfI	GANTC	7 cut(s)	153, 389, 614, 620, 863, 1719, 1970
HpaII	CCGG	2 cut(s)	64, 2402
HphI	GGTGA	8 cut(s)	235, 434, 1089, 1453, 1535, 2266, 2276, 2453
Hpy166II	GTNNAC	3 cut(s)	1629, 1718, 2012
Hpy8I	GTNNAC	3 cut(s)	1629, 1718, 2012
Hpy99I	CGWCG	1 cut(s)	450
HpyAV	CCTTC	4 cut(s)	57, 1617, 2255, 2476
HpyCH4IV	ACGT	4 cut(s)	131, 250, 934, 2030
HpyF3I	CTNAG	9 cut(s)	464, 528, 594, 622, 840, 1161, 1413, 1557, 1974
HpySE526I	ACGT	4 cut(s)	131, 250, 934, 2030
Kpn2I	TCCGGA	1 cut(s)	2401
Ksp22I	TGATCA	2 cut(s)	403, 889
LguI	GCTCTTC	1 cut(s)	621
LmnI	GCTCC	4 cut(s)	175, 441, 849, 1055
Lsp1109I	GCAGC	5 cut(s)	1309, 1396, 1619, 1729, 2287
MaeI	CTAG	8 cut(s)	354, 828, 1074, 1763, 1767, 1791, 1905, 2244
MaeII	ACGT	4 cut(s)	131, 250, 934, 2030
MboII	GAAGA	7 cut(s)	464, 638, 653, 1743, 1859, 1862, 2329
MfeI	CAATTG	1 cut(s)	39
MflI	RGATCY	5 cut(s)	493, 722, 800, 1461, 2003
MhlI	GDGCHC	5 cut(s)	346, 438, 633, 846, 1631
MlyI	GAGTC	3 cut(s)	614, 1713, 1964
MmeI	TCCRAC	2 cut(s)	618, 1607
Mph1103I	ATGCAT	1 cut(s)	663
MroI	TCCGGA	1 cut(s)	2401
MroXI	GAANNNNTTC	1 cut(s)	2239
MseI	TTAA	5 cut(s)	744, 974, 1218, 1320, 2471
MslI	CAYNNNNRTG	5 cut(s)	447, 735, 1075, 1887, 2252
MspA1I	CMGCKG	1 cut(s)	1300
MspI	CCGG	2 cut(s)	64, 2402
MspR9I	CCNGG	2 cut(s)	1146, 1466
MunI	CAATTG	1 cut(s)	39
MvaI	CCWGG	2 cut(s)	1146, 1466
NlaIV	GGNNCC	8 cut(s)	171, 790, 802, 1227, 1840, 1873, 2014, 2484
NmeAIII	GCCGAG	1 cut(s)	1625
NmuCI	GTSAC	7 cut(s)	102, 223, 586, 1123, 1961, 1967, 2254
NsiI	ATGCAT	1 cut(s)	663
NspI	RCATGY	4 cut(s)	487, 520, 677, 1714
OliI	CACNNNNGTG	1 cut(s)	447
PaeI	GCATGC	2 cut(s)	487, 677
PaeR7I	CTCGAG	1 cut(s)	1821
PagI	TCATGA	3 cut(s)	927, 946, 2347
PceI	AGGCCT	1 cut(s)	1149
PciI	ACATGT	1 cut(s)	516
PciSI	GCTCTTC	1 cut(s)	621
PdmI	GAANNNNTTC	1 cut(s)	2239
PfeI	GAWTC	4 cut(s)	153, 389, 614, 863
Pfl23II	CGTACG	1 cut(s)	128
PflFI	GACNNNGTC	1 cut(s)	2029
PflMI	CCANNNNNTGG	3 cut(s)	1465, 1487, 2500
PfoI	TCCNGGA	1 cut(s)	1464
PkrI	GCNGC	7 cut(s)	352, 1299, 1411, 1609, 1744, 1747, 2277
PleI	GAGTC	3 cut(s)	614, 1713, 1964
PmaCI	CACGTG	1 cut(s)	935
PmlI	CACGTG	1 cut(s)	935
PpsI	GAGTC	3 cut(s)	614, 1713, 1964
Ppu21I	YACGTR	1 cut(s)	935
PscI	ACATGT	1 cut(s)	516
Psp124BI	GAGCTC	3 cut(s)	438, 633, 846
Psp1406I	AACGTT	1 cut(s)	250
Psp6I	CCWGG	2 cut(s)	1144, 1464
PspCI	CACGTG	1 cut(s)	935
PspFI	CCCAGC	1 cut(s)	2420
PspGI	CCWGG	2 cut(s)	1144, 1464
PspLI	CGTACG	1 cut(s)	128
PspN4I	GGNNCC	8 cut(s)	171, 790, 802, 1227, 1840, 1873, 2014, 2484
PspPI	GGNCC	3 cut(s)	44, 1226, 2012
PstNI	CAGNNNCTG	1 cut(s)	1465
PsuI	RGATCY	5 cut(s)	493, 722, 800, 1461, 2003
PsyI	GACNNNGTC	1 cut(s)	2029
PvuII	CAGCTG	1 cut(s)	1300
RsaI	GTAC	4 cut(s)	130, 1562, 1771, 2167
RsaNI	GTAC	4 cut(s)	129, 1561, 1770, 2166
RseI	CAYNNNNRTG	5 cut(s)	447, 735, 1075, 1887, 2252
SacI	GAGCTC	3 cut(s)	438, 633, 846
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	5 cut(s)	744, 974, 1218, 1320, 2471
SatI	GCNGC	7 cut(s)	351, 1298, 1410, 1608, 1743, 1746, 2276
Sau96I	GGNCC	3 cut(s)	44, 1226, 2012
ScaI	AGTACT	1 cut(s)	1562
SchI	GAGTC	3 cut(s)	614, 1713, 1964
ScrFI	CCNGG	2 cut(s)	1146, 1466
SduI	GDGCHC	5 cut(s)	346, 438, 633, 846, 1631
SfcI	CTRYAG	1 cut(s)	148
Sfr274I	CTCGAG	1 cut(s)	1821
SinI	GGWCC	3 cut(s)	44, 1226, 2012
SlaI	CTCGAG	1 cut(s)	1821
SmiMI	CAYNNNNRTG	5 cut(s)	447, 735, 1075, 1887, 2252
SmlI	CTYRAG	4 cut(s)	109, 1509, 1821, 2158
SmoI	CTYRAG	4 cut(s)	109, 1509, 1821, 2158
SpeI	ACTAGT	1 cut(s)	1904
SphI	GCATGC	2 cut(s)	487, 677
SseBI	AGGCCT	1 cut(s)	1149
SsiI	CCGC	3 cut(s)	351, 900, 1745
SspI	AATATT	2 cut(s)	123, 2311
SspMI	CTAG	8 cut(s)	354, 828, 1074, 1763, 1767, 1791, 1905, 2244
SstI	GAGCTC	3 cut(s)	438, 633, 846
StuI	AGGCCT	1 cut(s)	1149
StyD4I	CCNGG	2 cut(s)	1144, 1464
StyI	CCWWGG	3 cut(s)	205, 1055, 1615
TaiI	ACGT	4 cut(s)	134, 253, 937, 2033
TaqI	TCGA	6 cut(s)	196, 756, 1027, 1241, 1822, 2049
TatI	WGTACW	2 cut(s)	1560, 1769
TauI	GCSGC	2 cut(s)	353, 1748
TfiI	GAWTC	4 cut(s)	153, 389, 614, 863
Tru1I	TTAA	5 cut(s)	744, 974, 1218, 1320, 2471
Tru9I	TTAA	5 cut(s)	744, 974, 1218, 1320, 2471
TscAI	CASTG	9 cut(s)	28, 756, 799, 1128, 1254, 1789, 1972, 2374, 2505
TseFI	GTSAC	7 cut(s)	102, 223, 586, 1123, 1961, 1967, 2254
TseI	GCWGC	5 cut(s)	1297, 1409, 1607, 1742, 2275
Tsp45I	GTSAC	7 cut(s)	102, 223, 586, 1123, 1961, 1967, 2254
TspDTI	ATGAA	9 cut(s)	398, 402, 539, 646, 678, 935, 1904, 2035, 2490
TspGWI	ACGGA	2 cut(s)	21, 2495
TspRI	CASTG	9 cut(s)	28, 756, 799, 1128, 1254, 1789, 1972, 2374, 2505
Tth111I	GACNNNGTC	1 cut(s)	2029
Van91I	CCANNNNNTGG	3 cut(s)	1465, 1487, 2500
VneI	GTGCAC	1 cut(s)	1627
VpaK11BI	GGWCC	3 cut(s)	44, 1226, 2012
XagI	CCTNNNNNAGG	2 cut(s)	1154, 2020
XapI	RAATTY	8 cut(s)	296, 1029, 1379, 1778, 1797, 1828, 2062, 2198
XceI	RCATGY	4 cut(s)	487, 520, 677, 1714
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1254, 1889
XhoI	CTCGAG	1 cut(s)	1821
XmiI	GTMKAC	1 cut(s)	1717
XmnI	GAANNNNTTC	1 cut(s)	2239
XspI	CTAG	8 cut(s)	354, 828, 1074, 1763, 1767, 1791, 1905, 2244
ZrmI	AGTACT	1 cut(s)	1562
Zsp2I	ATGCAT	1 cut(s)	663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.