Rorug02G0252800

Triacylglycerol lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007372
Physical Location & Seq
Reverse (-)
26454684 .. 26456797
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0252800.1

Sequence Viewer

Length: 531 bp
ATGGACAAGGAAGATGGCACAAGGTTCCTCTCCAAGCAGCCAAACATTAAGAGAGGAGACTTTGCAGAGGATGAAGTCGATCTAATGATTAGGCTTCGGAAGCTTTTGGGAAACAGGTGGTCTTTGATTGCTGGACGACTTCCTGGAAGAACATCAAACGATGTGAAAAACTATTGGAGTGCTCGAGGAAGAAGAAACATAAATTTTTGCTTCACAAAAAATAATAATCCTCCAGAAATAACAAAGACCACAGTAATAAGACCTCGACCACGAACCTTCACCAAAAGTCTACATTATTTAAGTGCAAAAGCTACAACTTCAAAACCTATTAATTTAGAAAATAGTTCATCGTCAACATCACCACCTATAGAGAATGGAAATGAGGAGTGGAAAACTCTACTGGAGGAAGATGTCCTTACAAACTTTTGGAATGAGGTTACAGTCTCAATGTCAAGTATGGGTGTTAATTCTACTCAACAAGGTTTTGAAATGGACCTTTGGCATTTTCTACAAGAAGAAGCAAGGCAATAA

Protein Analysis

176

Amino Acids

20.3

Weight (kDa)

9.09

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 18 - 59 4.1e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 289
AcsI RAATTY 1 cut(s) 202
AgsI TTSAA 2 cut(s) 321, 488
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 2 cut(s) 103, 311
AluI AGCT 2 cut(s) 103, 311
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 51, 448
Ama87I CYCGRG 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 202
AseI ATTAAT 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 271, 351
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 1 cut(s) 493
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 49
BccI CCATC 1 cut(s) 8
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 51, 448
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 493
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 1 cut(s) 144
BpmI CTGGAG 2 cut(s) 216, 422
Bse1I ACTGG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 76
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 2 cut(s) 69, 398
BseXI GCAGC 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 183
BsmAI GTCTC 2 cut(s) 51, 448
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 26
BsrI ACTGG 1 cut(s) 405
BssMI GATC 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 253, 442
BstF5I GGATG 1 cut(s) 76
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 2 cut(s) 51, 448
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 49
BtsCI GGATG 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 493
CviJI RGCY 4 cut(s) 40, 94, 103, 311
CviKI_1 RGCY 4 cut(s) 40, 94, 103, 311
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 493
Eco88I CYCGRG 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 142
FaiI YATR 3 cut(s) 200, 368, 458
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 1 cut(s) 38
GluI GCNGC 1 cut(s) 38
GsuI CTGGAG 2 cut(s) 216, 422
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HindIII AAGCTT 1 cut(s) 101
HphI GGTGA 2 cut(s) 271, 351
Hpy166II GTNNAC 2 cut(s) 290, 354
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 2 cut(s) 290, 354
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 253, 442
HpyCH4V TGCA 2 cut(s) 65, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
Kzo9I GATC 1 cut(s) 79
LpnPI CCDG 6 cut(s) 100, 117, 129, 156, 246, 386
Lsp1109I GCAGC 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 436
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 6 cut(s) 23, 159, 201, 204, 419, 527
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 3 cut(s) 202, 331, 466
MnlI CCTC 9 cut(s) 38, 47, 61, 179, 240, 273, 376, 397, 427
MseI TTAA 4 cut(s) 48, 299, 330, 465
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 100
NdeII GATC 1 cut(s) 79
NlaIV GGNNCC 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 183
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 1 cut(s) 39
PshBI ATTAAT 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 1 cut(s) 493
PspXI VCTCGAGB 1 cut(s) 183
SaqAI TTAA 4 cut(s) 48, 299, 330, 465
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 1 cut(s) 493
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 1 cut(s) 184
SfcI CTRYAG 1 cut(s) 366
Sfr274I CTCGAG 1 cut(s) 183
SinI GGWCC 1 cut(s) 493
SlaI CTCGAG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
Sse9I AATT 3 cut(s) 202, 331, 466
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 253, 442
TaqI TCGA 3 cut(s) 78, 184, 265
TasI AATT 3 cut(s) 202, 331, 466
Tru1I TTAA 4 cut(s) 48, 299, 330, 465
Tru9I TTAA 4 cut(s) 48, 299, 330, 465
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 87, 336
VpaK11BI GGWCC 1 cut(s) 493
VspI ATTAAT 1 cut(s) 330
XapI RAATTY 1 cut(s) 202
XhoI CTCGAG 1 cut(s) 183
XmiI GTMKAC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.