Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007372

Physical Location & Seq

Genomic Coordinates Forward (+)

9790529 .. 9793961

Gene Structure 3433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1083100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGATATAAGTAATGAGGCCAGTGTTGAACCATTTCCTATTGGACCTTCAACCATTGTTGGCCGGACGATCGCTTTCAAGGTTTTATTTTGCAAGTCAATGTCACATTTGAGGCATCAAATATTTCGTATCTGTTTGAGTCTCGTCTATAGATTCAAGAACTTTTTGGCTCCCATGTTCTCATGGTTGCATCCCCGAAACCCTCAAGGGATATTGGCAATGGTGACAATCATTGCTTTTCTGTTGAAACGATATACGAATGTCAAAGTCAAGGCGGAAATGGCTTATCGAAGGAAATTCTGGAGAAATATGATGAGAACGGCATTGACCTATGAGGAGTGGGCTCATGCTGCTAGGATGCTTGATAAAGAGGCACCGAAGATGAATGAATCAGACCTTTATGACGAAGAAGTAGTTAGGAACAAGCTTCAAGAGCTCCACCACCGTCGTGAAGAGGGTTCTCTCCGAGATATCATGTTCTGCATGAGAGCAGATCTTGTTAGAAATCTCGGTAATATGTGCAACCCTGAGCTTCACAAGGAGAAACTTCATGTTCCCAAACTCATCAAGGAATACATACATGAGGTCTCAACTCAGTTGAGAATGGTTTGTGATTCAGACTCAGAAGAGCTCTCGTTGGAAGAAAAGCTGGCTTTCATGCATGAAACTAGACATGCTTTTGGGAGAACTGCCTTGCTCTTGAGTGGGGGCGCTTCTCTTGGGTCTTTCCATGTTGGTGTGGTTAAAACGTTGGTCGAACATAAGCTTTTGCCGAGAATAATTGCTGGTTCCAGTGTAGGATCCATTATGTGTTCTGTTGTTGCCACTAGGTCTTGGCCTGAGCTCCAGAGCTTTTTTGAGGATTCTTGGCACTCATTGCAGTTTTTTGATCAGATGGGTGGGATTTTTACAGTTGTCAAGAGGGTCATGACACGTGGGGCAGTTCATGAGATCAGGCAGTTACAAATGATGCTAAGGCATCTAACGAGTAACCTGACGTTTCAAGAAGCTTATGACATGACAGGTCGAATTCTTGGGATAACTGTTTGCTCGCCGAGGAAGCATGAGCCACCTAGATGCCTTAATTACTTGACTTCACCTCATGTTGTCATATGGAGTGCAGTGACAGCTTCTTGTGCTTTTCCTGGCCTTTTTGAGGCTCAGGAACTAATGGCAAAGGATAGAAGTGGAGAGATTGTTCCATATCATCCTCCTTTTAATTTGGGTCCAGAGGAAGGGTCGATGCCAGTGCGTAGGTGGAGGGATGGTAGCTTGGAGATAGATTTACCTATGATGCAACTGAAAGAACTGTTCAACGTTAATCATTTTATTGTCAGTCAGGCAAATCCTCATATTGCACCATTGTTGAGGCTAAAGGAATTTGTGAGAGCTTGTGGAGGAAACTTTGCTGCTAAGCTTGCTCATCTTGCTGAAATGGAGGTGAAGCATAGATGTAATCAGATCCTGGAACTTGGTTTTCCATTAGGTGGACTTGCCAAGCTGTTCGCTCAAGATTGGGAGGGTGACGTTACTGTTGTTATGCCAGCTACACTTGCTCAGTACTCTAAAATTATACAAAACCCAACACATATTGAGCTTCAAAAGGCAGCCAACCAAGGTAGAAGGTGCACTTGGGAGAAGCTCTCGGCCATAAAAGCAAACTGTGGCATTGAGCTTGCTCTTGATGAGTGTGTTGTAATTCTCAACCACATGCGTAGACTCAAAAGGAGTGCAGAAAGAGCTGCGGCTTCTTCTTCTTCTCATGGCCTAGCTAGTACAAACAAATTCAGTGCATCCAGAAGAATTCCGTCTTGGAATTGCATTGCTCGAGAGAATTCAAGTGGTTCCCTTGAAGAAGACCTCATTGGAGATGGTGGTTCCTCATTTCATCATGGTGTTGGCGCATCTACCAGTGGAGTTCATTCTGGTAAAAGCTTTCAAACCCACCGTAATATACATGATGGAAGTGACAGTGAATGCGAGAGCATTGATTTTAATTCCTGGACAATATCTGGTGGACCCTTGATGAGGACAACATCAGCAAATAAACTCATCGACTTTGTCCAAGATTTGGACATTGATGCTGAATTGAACAGAAGTGTGCTGACTAATCCTAACTCAATGACGCTTCAGATGGGAGGCAATAATCATTACTATCAAAGCCCGAGAGATACAACACCTGAAAGAAGTCCTGAAAGCATAAAATTTGATAAGAGGGAGTTTGGAAGCATGGTTTCAGCAAATGGTTCTAGCATCACGGTGACTGAAGGTGATCTGTTGCAGCCTGAAAGGATACATAATGGGATTGTATTTAATGTTGTGAAGAAAGAAACATTGTCAAGTAGGAGTAGTAATGACGGGGAAAATTACGGGAGTGAAGAAGCTGAATGTGTGCAACTTGACGTTCTGGAAAATGACATGGACGCTAGGTCTGCATATGAAGATGATGATGCCGATATCACTTCCGCAGCCTTCTTAAATGAAACGGAACCCGATTGCCTGTCAACGGATCAACCTAAGGAAGAAGATTGTAATCATCAGAGCACTGTGGATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004806 GO:0005575 GO:0005622 GO:0005623 GO:0005811 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0012511 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0019433 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044424 GO:0044464 GO:0046461 GO:0046464 GO:0046486 GO:0046503 GO:0052689 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00100 ko00561 ko00564 ko00565 ko00590 ko00591 ko00592 ko01100 ko01110 map00100 map00561 map00564 map00565 map00590 map00591 map00592 map01100 map01110

Pfam Domains

Protein Families

DUF3336 Patatin

Protein Analysis

853

Amino Acids

95.73

Weight (kDa)

6.16

Isoelectric Point (pI)

50.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3336	PF11815	94 - 225	1.3e-31	Domain of unknown function (DUF3336)
Patatin	PF01734	232 - 404	2.2e-18	Patatin-like phospholipase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007372)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04040 AT5G04040
fragaria_vesca	FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040 FvH4_6g25040
malus_domestica	MD12G1087400.v1.1 MD14G1083100.v1.1
prunus_persica	Prupe.7G047400_v2.0.a1
pyrus_communis	pycom12g07540 pycom14g07220
rosa_chinensis	RchiOBHm_Chr2g0124811
rosa_laevigata	RLG00000018823
rosa_multiflora	Rmu_sc0002939.1_g000008
rosa_roxburghii	Rroxscaffold_2G00119660
rosa_rugosa	Rorug02G0252600 Rorug02G0252700 Rorug02G0252800
rosa_samantha	Rh2AG312300 Rh2BG320500 Rh2CG300500 Rh2DG337700
rosa_wichuraiana	Rw2G025220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	373
AccB7I	CCANNNNNTGG	2 cut(s)	1487, 2071
AccI	GTMKAC	1 cut(s)	1717
AciI	CCGC	3 cut(s)	275, 1745, 2466
AclI	AACGTT	2 cut(s)	749, 1317
AclWI	GGATC	4 cut(s)	795, 808, 1456, 2517
AcoI	YGGCCR	2 cut(s)	61, 1647
AcsI	RAATTY	7 cut(s)	296, 1029, 1379, 1784, 1803, 1834, 2204
AcuI	CTGAAG	2 cut(s)	2114, 2286
AcvI	CACGTG	1 cut(s)	935
AfaI	GTAC	2 cut(s)	1562, 1777
AfiI	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1487, 2028, 2071, 2506
AflIII	ACRYGT	1 cut(s)	932
AhdI	GACNNNNNGTC	1 cut(s)	2428
AjnI	CCWGG	3 cut(s)	1144, 1464, 2000
AjuI	GAANNNNNNNTTGG	6 cut(s)	1615, 1647, 1795, 1827, 1848, 1880
AleI	CACNNNNGTG	1 cut(s)	447
AloI	GAACNNNNNNTCC	2 cut(s)	1182, 1214
Alw21I	GWGCWC	5 cut(s)	438, 633, 846, 1631, 2546
Alw26I	GTCTC	2 cut(s)	146, 592
Alw44I	GTGCAC	1 cut(s)	1627
AlwI	GGATC	4 cut(s)	795, 808, 1456, 2517
AlwNI	CAGNNNCTG	1 cut(s)	1465
Ama87I	CYCGRG	2 cut(s)	1827, 2164
AoxI	GGCC	6 cut(s)	18, 61, 836, 1147, 1647, 1765
ApaLI	GTGCAC	1 cut(s)	1627
ApeKI	GCWGC	6 cut(s)	350, 1409, 1607, 1742, 2281, 2468
ApoI	RAATTY	7 cut(s)	296, 1029, 1379, 1784, 1803, 1834, 2204
Asp700I	GAANNNNTTC	1 cut(s)	33
AspLEI	GCGC	2 cut(s)	713, 1904
AspS9I	GGNCC	3 cut(s)	44, 1226, 2018
AsuHPI	GGTGA	6 cut(s)	235, 1089, 1453, 1535, 2272, 2282
AvaI	CYCGRG	2 cut(s)	1827, 2164
AvaII	GGWCC	3 cut(s)	44, 1226, 2018
AxyI	CCTNAGG	1 cut(s)	2517
BaeGI	GKGCMC	1 cut(s)	1631
BamHI	GGATCC	1 cut(s)	800
BanI	GGYRCC	1 cut(s)	373
BanII	GRGCYC	4 cut(s)	346, 438, 633, 846
BbrPI	CACGTG	1 cut(s)	935
BbsI	GAAGAC	1 cut(s)	1863
Bbv12I	GWGCWC	5 cut(s)	438, 633, 846, 1631, 2546
BbvI	GCAGC	6 cut(s)	337, 1396, 1619, 1729, 2293, 2480
BccI	CCATC	6 cut(s)	889, 1259, 1865, 1955, 2128, 2546
BceAI	ACGGC	1 cut(s)	336
BcgI	CGANNNNNNTGC	2 cut(s)	1231, 1265
BciT130I	CCWGG	3 cut(s)	1146, 1466, 2002
BciVI	GTATCC	1 cut(s)	2286
BclI	TGATCA	1 cut(s)	889
BcoDI	GTCTC	2 cut(s)	146, 592
BfaI	CTAG	8 cut(s)	354, 669, 828, 1074, 1769, 1773, 2250, 2427
BfmI	CTRYAG	1 cut(s)	148
BfoI	RGCGCY	1 cut(s)	714
BfuI	GTATCC	1 cut(s)	2286
BglII	AGATCT	1 cut(s)	493
BisI	GCNGC	7 cut(s)	351, 1410, 1608, 1743, 1746, 2282, 2469
BlpI	GCTNAGC	1 cut(s)	1413
BlsI	GCNGC	7 cut(s)	352, 1411, 1609, 1744, 1747, 2283, 2470
BmcAI	AGTACT	1 cut(s)	1562
Bme1390I	CCNGG	3 cut(s)	1146, 1466, 2002
Bme18I	GGWCC	3 cut(s)	44, 1226, 2018
BmeRI	GACNNNNNGTC	1 cut(s)	2428
BmeT110I	CYCGRG	2 cut(s)	1827, 2164
BmgT120I	GGNCC	3 cut(s)	44, 1226, 2018
BmiI	GGNNCC	9 cut(s)	171, 375, 790, 802, 1227, 1846, 1879, 2020, 2490
BmrFI	CCNGG	3 cut(s)	1146, 1466, 2002
BpiI	GAAGAC	1 cut(s)	1863
BplI	GAGNNNNNCTC	2 cut(s)	1628, 1660
BpmI	CTGGAG	2 cut(s)	322, 830
Bpu10I	CCTNAGC	4 cut(s)	528, 840, 974, 1161
Bpu1102I	GCTNAGC	1 cut(s)	1413
BpuEI	CTTGAG	3 cut(s)	189, 721, 1494
BsaAI	YACGTR	1 cut(s)	935
BsaBI	GATNNNNATC	1 cut(s)	2532
BsaI	GGTCTC	1 cut(s)	592
BsaJI	CCNNGG	2 cut(s)	1055, 1615
BsaXI	ACNNNNNCTCC	4 cut(s)	1182, 1212, 1252, 1282
Bsc4I	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1487, 2028, 2071, 2506
Bse1I	ACTGG	4 cut(s)	21, 792, 1247, 1911
Bse21I	CCTNAGG	1 cut(s)	2517
Bse3DI	GCAATG	4 cut(s)	225, 231, 875, 1821
Bse8I	GATNNNNATC	1 cut(s)	2532
BseBI	CCWGG	3 cut(s)	1146, 1466, 2002
BseDI	CCNNGG	2 cut(s)	1055, 1615
BseGI	GGATG	6 cut(s)	190, 363, 1207, 1270, 1793, 2557
BseJI	GATNNNNATC	1 cut(s)	2532
BseLI	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1487, 2028, 2071, 2506
BseMI	GCAATG	4 cut(s)	225, 231, 875, 1821
BseMII	CTCAG	6 cut(s)	519, 608, 636, 831, 1175, 1571
BseNI	ACTGG	4 cut(s)	21, 792, 1247, 1911
BseRI	GAGGAG	1 cut(s)	350
BseSI	GKGCMC	1 cut(s)	1631
BseXI	GCAGC	6 cut(s)	337, 1396, 1619, 1729, 2293, 2480
BsgI	GTGCAG	2 cut(s)	1140, 1752
Bsh1285I	CGRYCG	1 cut(s)	72
BshFI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1767
BshNI	GGYRCC	1 cut(s)	373
BsiEI	CGRYCG	1 cut(s)	72
BsiHKAI	GWGCWC	5 cut(s)	438, 633, 846, 1631, 2546
BsiHKCI	CYCGRG	2 cut(s)	1827, 2164
BsiSI	CCGG	1 cut(s)	64
BslI	CCNNNNNNNGG	6 cut(s)	1156, 1235, 1487, 2028, 2071, 2506
BsmAI	GTCTC	2 cut(s)	146, 592
BsmI	GAATGC	1 cut(s)	1982
BsnI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1767
Bso31I	GGTCTC	1 cut(s)	592
BsoBI	CYCGRG	2 cut(s)	1827, 2164
Bsp1286I	GDGCHC	6 cut(s)	346, 438, 633, 846, 1631, 2546
Bsp143I	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
Bsp1720I	GCTNAGC	1 cut(s)	1413
BspACI	CCGC	3 cut(s)	275, 1745, 2466
BspANI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1767
BspCNI	CTCAG	6 cut(s)	520, 607, 635, 832, 1174, 1570
BspHI	TCATGA	2 cut(s)	927, 946
BspLI	GGNNCC	9 cut(s)	171, 375, 790, 802, 1227, 1846, 1879, 2020, 2490
BspPI	GGATC	4 cut(s)	795, 808, 1456, 2517
BspQI	GCTCTTC	1 cut(s)	621
BspT107I	GGYRCC	1 cut(s)	373
BspTNI	GGTCTC	1 cut(s)	592
BsrDI	GCAATG	4 cut(s)	225, 231, 875, 1821
BsrI	ACTGG	4 cut(s)	21, 792, 1247, 1911
BssECI	CCNNGG	2 cut(s)	1055, 1615
BssMI	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
BssT1I	CCWWGG	1 cut(s)	1615
Bst2UI	CCWGG	3 cut(s)	1146, 1466, 2002
Bst6I	CTCTTC	2 cut(s)	447, 621
BstAPI	GCANNNNNTGC	1 cut(s)	877
BstBAI	YACGTR	1 cut(s)	935
BstC8I	GCNNGC	5 cut(s)	651, 1052, 1419, 1545, 1677
BstDEI	CTNAG	9 cut(s)	528, 594, 622, 840, 974, 1161, 1413, 1557, 2517
BstENI	CCTNNNNNAGG	2 cut(s)	1154, 2026
BstF5I	GGATG	6 cut(s)	190, 363, 1207, 1270, 1793, 2557
BstH2I	RGCGCY	1 cut(s)	714
BstHHI	GCGC	2 cut(s)	713, 1904
BstKTI	GATC	8 cut(s)	72, 496, 803, 892, 954, 1464, 2275, 2512
BstMAI	GTCTC	2 cut(s)	146, 592
BstMBI	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
BstMCI	CGRYCG	1 cut(s)	72
BstNI	CCWGG	3 cut(s)	1146, 1466, 2002
BstNSI	RCATGY	2 cut(s)	677, 1714
BstSCI	CCNGG	3 cut(s)	1144, 1464, 2000
BstSFI	CTRYAG	1 cut(s)	148
BstSLI	GKGCMC	1 cut(s)	1631
BstV1I	GCAGC	6 cut(s)	337, 1396, 1619, 1729, 2293, 2480
BstV2I	GAAGAC	1 cut(s)	1863
BstX2I	RGATCY	3 cut(s)	493, 800, 1461
BstYI	RGATCY	3 cut(s)	493, 800, 1461
Bsu36I	CCTNAGG	1 cut(s)	2517
BsuI	GTATCC	1 cut(s)	2286
BsuRI	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1767
BtsCI	GGATG	6 cut(s)	190, 363, 1207, 1270, 1793, 2557
BtsI	GCAGTG	1 cut(s)	1128
BtsIMutI	CAGTG	8 cut(s)	28, 799, 1128, 1254, 1795, 1918, 1978, 2544
Cac8I	GCNNGC	5 cut(s)	651, 1052, 1419, 1545, 1677
CaiI	CAGNNNCTG	1 cut(s)	1465
CciI	TCATGA	2 cut(s)	927, 946
CfoI	GCGC	2 cut(s)	713, 1904
Cfr13I	GGNCC	3 cut(s)	44, 1226, 2018
CseI	GACGC	2 cut(s)	2134, 2432
Csp6I	GTAC	2 cut(s)	1561, 1776
CviQI	GTAC	2 cut(s)	1561, 1776
DdeI	CTNAG	9 cut(s)	528, 594, 622, 840, 974, 1161, 1413, 1557, 2517
DpnI	GATC	8 cut(s)	71, 495, 802, 891, 953, 1463, 2274, 2511
DpnII	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
DriI	GACNNNNNGTC	1 cut(s)	2428
EaeI	YGGCCR	2 cut(s)	61, 1647
Eam1104I	CTCTTC	2 cut(s)	447, 621
Eam1105I	GACNNNNNGTC	1 cut(s)	2428
EarI	CTCTTC	2 cut(s)	447, 621
EciI	GGCGGA	1 cut(s)	290
Ecl136II	GAGCTC	3 cut(s)	436, 631, 844
Eco130I	CCWWGG	1 cut(s)	1615
Eco24I	GRGCYC	4 cut(s)	346, 438, 633, 846
Eco31I	GGTCTC	1 cut(s)	592
Eco32I	GATATC	2 cut(s)	472, 2458
Eco47I	GGWCC	3 cut(s)	44, 1226, 2018
Eco53kI	GAGCTC	3 cut(s)	436, 631, 844
Eco57I	CTGAAG	2 cut(s)	2114, 2286
Eco72I	CACGTG	1 cut(s)	935
Eco81I	CCTNAGG	1 cut(s)	2517
Eco88I	CYCGRG	2 cut(s)	1827, 2164
EcoICRI	GAGCTC	3 cut(s)	436, 631, 844
EcoNI	CCTNNNNNAGG	2 cut(s)	1154, 2026
EcoRI	GAATTC	3 cut(s)	1029, 1803, 1834
EcoRII	CCWGG	3 cut(s)	1144, 1464, 2000
EcoRV	GATATC	2 cut(s)	472, 2458
EcoT14I	CCWWGG	1 cut(s)	1615
EcoT22I	ATGCAT	1 cut(s)	663
EcoT38I	GRGCYC	4 cut(s)	346, 438, 633, 846
ErhI	CCWWGG	1 cut(s)	1615
FalI	AAGNNNNNCTT	2 cut(s)	1615, 1647
FauNDI	CATATG	2 cut(s)	1112, 2437
FbaI	TGATCA	1 cut(s)	889
FblI	GTMKAC	1 cut(s)	1717
Fnu4HI	GCNGC	7 cut(s)	351, 1410, 1608, 1743, 1746, 2282, 2469
FokI	GGATG	5 cut(s)	177, 370, 1194, 1277, 1780
FriOI	GRGCYC	4 cut(s)	346, 438, 633, 846
Fsp4HI	GCNGC	7 cut(s)	351, 1410, 1608, 1743, 1746, 2282, 2469
FspBI	CTAG	8 cut(s)	354, 669, 828, 1074, 1769, 1773, 2250, 2427
GlaI	GCGC	2 cut(s)	712, 1903
GluI	GCNGC	7 cut(s)	351, 1410, 1608, 1743, 1746, 2282, 2469
GsuI	CTGGAG	2 cut(s)	322, 830
HaeII	RGCGCY	1 cut(s)	714
HaeIII	GGCC	6 cut(s)	20, 63, 838, 1149, 1649, 1767
HapII	CCGG	1 cut(s)	64
HgaI	GACGC	2 cut(s)	2134, 2432
HhaI	GCGC	2 cut(s)	713, 1904
Hin6I	GCGC	2 cut(s)	711, 1902
HinP1I	GCGC	2 cut(s)	711, 1902
HincII	GTYRAC	1 cut(s)	2505
HindII	GTYRAC	1 cut(s)	2505
HindIII	AAGCTT	5 cut(s)	425, 764, 1008, 1415, 1933
HinfI	GANTC	7 cut(s)	139, 153, 389, 614, 620, 863, 1719
HpaII	CCGG	1 cut(s)	64
HphI	GGTGA	6 cut(s)	235, 1089, 1453, 1535, 2272, 2282
Hpy166II	GTNNAC	5 cut(s)	1490, 1629, 1718, 2018, 2505
Hpy188I	TCNGA	8 cut(s)	394, 467, 619, 625, 894, 1461, 2133, 2541
Hpy8I	GTNNAC	5 cut(s)	1490, 1629, 1718, 2018, 2505
Hpy99I	CGWCG	1 cut(s)	450
HpyAV	CCTTC	6 cut(s)	57, 285, 1229, 1617, 2261, 2482
HpyCH4IV	ACGT	6 cut(s)	749, 934, 998, 1317, 1527, 2403
HpyF3I	CTNAG	9 cut(s)	528, 594, 622, 840, 974, 1161, 1413, 1557, 2517
HpySE526I	ACGT	6 cut(s)	749, 934, 998, 1317, 1527, 2403
HspAI	GCGC	2 cut(s)	711, 1902
Ksp22I	TGATCA	1 cut(s)	889
Kzo9I	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
LguI	GCTCTTC	1 cut(s)	621
LmnI	GCTCC	3 cut(s)	175, 441, 849
Lsp1109I	GCAGC	6 cut(s)	337, 1396, 1619, 1729, 2293, 2480
MaeI	CTAG	8 cut(s)	354, 669, 828, 1074, 1769, 1773, 2250, 2427
MaeII	ACGT	6 cut(s)	749, 934, 998, 1317, 1527, 2403
MaeIII	GTNAC	9 cut(s)	102, 223, 960, 989, 1123, 1523, 1528, 1967, 2260
MalI	GATC	8 cut(s)	71, 495, 802, 891, 953, 1463, 2274, 2511
MboI	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
MflI	RGATCY	3 cut(s)	493, 800, 1461
MhlI	GDGCHC	6 cut(s)	346, 438, 633, 846, 1631, 2546
MlyI	GAGTC	3 cut(s)	148, 614, 1713
MmeI	TCCRAC	1 cut(s)	618
Mph1103I	ATGCAT	1 cut(s)	663
MroXI	GAANNNNTTC	1 cut(s)	33
MseI	TTAA	7 cut(s)	744, 1083, 1218, 1320, 1995, 2313, 2477
MslI	CAYNNNNRTG	5 cut(s)	447, 735, 1075, 1893, 2258
MspI	CCGG	1 cut(s)	64
MspR9I	CCNGG	3 cut(s)	1146, 1466, 2002
Mva1269I	GAATGC	1 cut(s)	1982
MvaI	CCWGG	3 cut(s)	1146, 1466, 2002
NdeI	CATATG	2 cut(s)	1112, 2437
NdeII	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
NlaIV	GGNNCC	9 cut(s)	171, 375, 790, 802, 1227, 1846, 1879, 2020, 2490
NmeAIII	GCCGAG	3 cut(s)	798, 1080, 1625
NmuCI	GTSAC	6 cut(s)	102, 223, 1123, 1523, 1967, 2260
NsiI	ATGCAT	1 cut(s)	663
NspI	RCATGY	2 cut(s)	677, 1714
OliI	CACNNNNGTG	1 cut(s)	447
PaeR7I	CTCGAG	1 cut(s)	1827
PagI	TCATGA	2 cut(s)	927, 946
PciSI	GCTCTTC	1 cut(s)	621
PctI	GAATGC	1 cut(s)	1982
PdmI	GAANNNNTTC	1 cut(s)	33
PfeI	GAWTC	4 cut(s)	153, 389, 614, 863
PflFI	GACNNNGTC	1 cut(s)	2060
PflMI	CCANNNNNTGG	2 cut(s)	1487, 2071
PfoI	TCCNGGA	2 cut(s)	1464, 2000
PkrI	GCNGC	7 cut(s)	352, 1411, 1609, 1744, 1747, 2283, 2470
Ple19I	CGATCG	1 cut(s)	72
PleI	GAGTC	3 cut(s)	147, 614, 1713
PmaCI	CACGTG	1 cut(s)	935
PmlI	CACGTG	1 cut(s)	935
PpsI	GAGTC	3 cut(s)	147, 614, 1713
Ppu21I	YACGTR	1 cut(s)	935
Psp124BI	GAGCTC	3 cut(s)	438, 633, 846
Psp1406I	AACGTT	2 cut(s)	749, 1317
Psp6I	CCWGG	3 cut(s)	1144, 1464, 2000
PspCI	CACGTG	1 cut(s)	935
PspGI	CCWGG	3 cut(s)	1144, 1464, 2000
PspN4I	GGNNCC	9 cut(s)	171, 375, 790, 802, 1227, 1846, 1879, 2020, 2490
PspPI	GGNCC	3 cut(s)	44, 1226, 2018
PstNI	CAGNNNCTG	1 cut(s)	1465
PsuI	RGATCY	3 cut(s)	493, 800, 1461
PsyI	GACNNNGTC	1 cut(s)	2060
PvuI	CGATCG	1 cut(s)	72
RsaI	GTAC	2 cut(s)	1562, 1777
RsaNI	GTAC	2 cut(s)	1561, 1776
RseI	CAYNNNNRTG	5 cut(s)	447, 735, 1075, 1893, 2258
SacI	GAGCTC	3 cut(s)	438, 633, 846
SapI	GCTCTTC	1 cut(s)	621
SaqAI	TTAA	7 cut(s)	744, 1083, 1218, 1320, 1995, 2313, 2477
SatI	GCNGC	7 cut(s)	351, 1410, 1608, 1743, 1746, 2282, 2469
Sau3AI	GATC	8 cut(s)	69, 493, 800, 889, 951, 1461, 2272, 2509
Sau96I	GGNCC	3 cut(s)	44, 1226, 2018
ScaI	AGTACT	1 cut(s)	1562
SchI	GAGTC	3 cut(s)	148, 614, 1713
ScrFI	CCNGG	3 cut(s)	1146, 1466, 2002
SduI	GDGCHC	6 cut(s)	346, 438, 633, 846, 1631, 2546
SfcI	CTRYAG	1 cut(s)	148
Sfr274I	CTCGAG	1 cut(s)	1827
SinI	GGWCC	3 cut(s)	44, 1226, 2018
SlaI	CTCGAG	1 cut(s)	1827
SmiMI	CAYNNNNRTG	5 cut(s)	447, 735, 1075, 1893, 2258
SmlI	CTYRAG	4 cut(s)	204, 700, 1509, 1827
SmoI	CTYRAG	4 cut(s)	204, 700, 1509, 1827
SsiI	CCGC	3 cut(s)	275, 1745, 2466
SspI	AATATT	1 cut(s)	123
SspMI	CTAG	8 cut(s)	354, 669, 828, 1074, 1769, 1773, 2250, 2427
SstI	GAGCTC	3 cut(s)	438, 633, 846
StyD4I	CCNGG	3 cut(s)	1144, 1464, 2000
StyI	CCWWGG	1 cut(s)	1615
TaiI	ACGT	6 cut(s)	752, 937, 1001, 1320, 1530, 2406
TaqI	TCGA	6 cut(s)	289, 756, 1027, 1241, 1828, 2055
TatI	WGTACW	2 cut(s)	1560, 1775
TauI	GCSGC	1 cut(s)	1748
TfiI	GAWTC	4 cut(s)	153, 389, 614, 863
Tru1I	TTAA	7 cut(s)	744, 1083, 1218, 1320, 1995, 2313, 2477
Tru9I	TTAA	7 cut(s)	744, 1083, 1218, 1320, 1995, 2313, 2477
TscAI	CASTG	8 cut(s)	28, 799, 1128, 1254, 1795, 1918, 1978, 2551
TseFI	GTSAC	6 cut(s)	102, 223, 1123, 1523, 1967, 2260
TseI	GCWGC	6 cut(s)	350, 1409, 1607, 1742, 2281, 2468
Tsp45I	GTSAC	6 cut(s)	102, 223, 1123, 1523, 1967, 2260
TspGWI	ACGGA	3 cut(s)	1797, 2501, 2522
TspRI	CASTG	8 cut(s)	28, 799, 1128, 1254, 1795, 1918, 1978, 2551
Tth111I	GACNNNGTC	1 cut(s)	2060
Van91I	CCANNNNNTGG	2 cut(s)	1487, 2071
VneI	GTGCAC	1 cut(s)	1627
VpaK11BI	GGWCC	3 cut(s)	44, 1226, 2018
XagI	CCTNNNNNAGG	2 cut(s)	1154, 2026
XapI	RAATTY	7 cut(s)	296, 1029, 1379, 1784, 1803, 1834, 2204
XceI	RCATGY	2 cut(s)	677, 1714
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1254
XhoI	CTCGAG	1 cut(s)	1827
XmiI	GTMKAC	1 cut(s)	1717
XmnI	GAANNNNTTC	1 cut(s)	33
XspI	CTAG	8 cut(s)	354, 669, 828, 1074, 1769, 1773, 2250, 2427
ZrmI	AGTACT	1 cut(s)	1562
Zsp2I	ATGCAT	1 cut(s)	663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.