AT5G04440

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008468
Physical Location & Seq
Forward (+)
1254757 .. 1257040
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04440.1

Sequence Viewer

Length: 768 bp
ATGGCGTTGAGCTCAGCTACGCTTAGACTTACCTCTCTGTCGTTCTCGGCGAAGAAGACGAATCGAAACCCTAGAAATCCAGCTCTCTCCTTCACCATCACTAGCTCATCGTCCTTTGACGAATCACCCAAACCTTCTTCTCTCGCCACTTCCTCTTCTACGTCACCTATACGCGTCTCCTCTAGTTCTACCCCCAAAGCTCGATTCATCGCTCGTCAGAAACAATCCGTCTCCGTTCGTCAGCTACAGCGACCTCTAATTGAGTATATGAGCTTACCTGCGAGTCAGTACTCGGTGCTTGATGCGGAGAGGATTGAGAGAGTTGATGATAACACGTTTAGATGTTATGTTTATACGTTTAAATTCTTCAACTTTGAAGTCTGCCCTGTTTTGCTTGTCAGGGTTGAAGAACAGCCTAATGGCTGCTGCATCAAGCTCTTGTCCTGCAAGCTTGAGGGATCTCCTGTTGTGGTTGCTCAAAATGACAAATTTGATGCTTCTATGGTGAACCGGGTATCATGTGATAGCACTCAGGAGGGCTCATCAGAGCAACAAATTACTTCAGATGCAGTCATTGAGGTCAATATCGAGATTCCTTTTGCATTTAGAGTCTTCCCAGTGGGAGCAATAGAGGCGACTGGGACACAAGTTCTTGATCAAATTCTTAAACTCATGCTACCTCGATTTTTGTCACAGCTCTCCAAAGACTATCATGCATGGGCTTCGGGAGACACTTCAAGGCAACCTCTTGGGACCGGTGAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

255

Amino Acids

28.16

Weight (kDa)

8.2

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 86 - 241 7.7e-44 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 286
AccII CGCG 1 cut(s) 174
AciI CCGC 1 cut(s) 305
AclWI GGATC 1 cut(s) 466
AcsI RAATTY 3 cut(s) 362, 488, 660
AcuI CTGAAG 1 cut(s) 546
AfaI GTAC 1 cut(s) 290
AflIII ACRYGT 2 cut(s) 172, 333
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 4 cut(s) 370, 377, 407, 738
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 3 cut(s) 181, 235, 723
AlwI GGATC 1 cut(s) 466
ApeKI GCWGC 2 cut(s) 423, 426
ApoI RAATTY 3 cut(s) 362, 488, 660
AsiGI ACCGGT 1 cut(s) 755
AspS9I GGNCC 1 cut(s) 753
AsuC2I CCSGG 1 cut(s) 512
AsuHPI GGTGA 4 cut(s) 85, 117, 156, 517
AvaII GGWCC 1 cut(s) 753
BanII GRGCYC 2 cut(s) 14, 542
BbsI GAAGAC 2 cut(s) 62, 604
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 410, 413
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 705, 739
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 1 cut(s) 512
BcoDI GTCTC 3 cut(s) 181, 235, 723
BfaI CTAG 3 cut(s) 72, 102, 183
BfmI CTRYAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 762
BisI GCNGC 2 cut(s) 424, 427
BlpI GCTNAGC 1 cut(s) 13
BlsI GCNGC 2 cut(s) 425, 428
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 512
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 1 cut(s) 753
BmiI GGNNCC 1 cut(s) 754
BmrFI CCNGG 1 cut(s) 512
BmrI ACTGGG 2 cut(s) 611, 648
BmsI GCATC 4 cut(s) 292, 438, 484, 556
BmuI ACTGGG 2 cut(s) 611, 648
BpiI GAAGAC 2 cut(s) 62, 604
Bpu1102I GCTNAGC 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 512
BsaWI WCCGGW 1 cut(s) 755
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 2 cut(s) 617, 643
BseMII CTCAG 2 cut(s) 27, 545
BseNI ACTGG 2 cut(s) 617, 643
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 2 cut(s) 410, 413
Bsh1236I CGCG 1 cut(s) 174
BshTI ACCGGT 1 cut(s) 755
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 2 cut(s) 511, 756
BslFI GGGAC 1 cut(s) 655
BsmAI GTCTC 3 cut(s) 181, 235, 723
BsmBI CGTCTC 2 cut(s) 181, 235
BsmFI GGGAC 1 cut(s) 655
Bsp1286I GDGCHC 2 cut(s) 14, 542
Bsp143I GATC 3 cut(s) 458, 655, 762
Bsp1720I GCTNAGC 1 cut(s) 13
BspACI CCGC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 26, 544
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 2 cut(s) 617, 643
BssAI RCCGGY 1 cut(s) 755
BssMI GATC 3 cut(s) 458, 655, 762
Bst6I CTCTTC 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 3 cut(s) 13, 23, 531
BstFNI CGCG 1 cut(s) 174
BstKTI GATC 3 cut(s) 461, 658, 765
BstMAI GTCTC 3 cut(s) 181, 235, 723
BstMBI GATC 3 cut(s) 458, 655, 762
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 510
BstSFI CTRYAG 1 cut(s) 245
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 2 cut(s) 410, 413
BstV2I GAAGAC 2 cut(s) 62, 604
BstX2I RGATCY 2 cut(s) 458, 762
BstYI RGATCY 2 cut(s) 458, 762
BtgZI GCGATG 1 cut(s) 193
BtsIMutI CAGTG 1 cut(s) 624
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 1 cut(s) 753
CseI GACGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 289
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 4 cut(s) 519, 673, 713, 717
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 3 cut(s) 13, 23, 531
DpnI GATC 3 cut(s) 460, 657, 764
DpnII GATC 3 cut(s) 458, 655, 762
DraI TTTAAA 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 14, 542
Eco47I GGWCC 1 cut(s) 753
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 2 cut(s) 14, 542
Esp3I CGTCTC 2 cut(s) 181, 235
FaeI CATG 4 cut(s) 522, 676, 716, 720
FaqI GGGAC 1 cut(s) 655
FatI CATG 4 cut(s) 518, 672, 712, 716
FbaI TGATCA 1 cut(s) 655
Fnu4HI GCNGC 2 cut(s) 424, 427
FriOI GRGCYC 2 cut(s) 14, 542
Fsp4HI GCNGC 2 cut(s) 424, 427
FspBI CTAG 3 cut(s) 72, 102, 183
GluI GCNGC 2 cut(s) 424, 427
HapII CCGG 2 cut(s) 511, 756
HgaI GACGC 1 cut(s) 163
Hin1II CATG 4 cut(s) 522, 676, 716, 720
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 6 cut(s) 61, 122, 204, 283, 592, 609
HpaII CCGG 2 cut(s) 511, 756
HphI GGTGA 4 cut(s) 85, 117, 156, 517
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 4 cut(s) 219, 547, 565, 767
Hpy188III TCNNGA 4 cut(s) 533, 589, 653, 726
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 2 cut(s) 100, 144
HpyCH4IV ACGT 3 cut(s) 161, 335, 356
HpyCH4V TGCA 5 cut(s) 429, 447, 569, 602, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 3 cut(s) 13, 23, 531
HpySE526I ACGT 3 cut(s) 161, 335, 356
Hsp92II CATG 4 cut(s) 522, 676, 716, 720
Ksp22I TGATCA 1 cut(s) 655
Kzo9I GATC 3 cut(s) 458, 655, 762
LmnI GCTCC 1 cut(s) 623
Lsp1109I GCAGC 2 cut(s) 410, 413
LweI GCATC 4 cut(s) 292, 438, 484, 556
MaeI CTAG 3 cut(s) 72, 102, 183
MaeII ACGT 3 cut(s) 161, 335, 356
MaeIII GTNAC 2 cut(s) 162, 690
MalI GATC 3 cut(s) 460, 657, 764
MboI GATC 3 cut(s) 458, 655, 762
MboII GAAGA 7 cut(s) 64, 67, 129, 147, 358, 419, 604
MflI RGATCY 2 cut(s) 458, 762
MhlI GDGCHC 2 cut(s) 14, 542
MluCI AATT 5 cut(s) 258, 362, 488, 555, 660
MluI ACGCGT 1 cut(s) 172
MlyI GAGTC 2 cut(s) 292, 618
Mph1103I ATGCAT 1 cut(s) 718
MseI TTAA 2 cut(s) 360, 666
MspI CCGG 2 cut(s) 511, 756
MspR9I CCNGG 1 cut(s) 512
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 632
NciI CCSGG 1 cut(s) 512
NdeII GATC 3 cut(s) 458, 655, 762
NlaIII CATG 4 cut(s) 522, 676, 716, 720
NlaIV GGNNCC 1 cut(s) 754
NmeAIII GCCGAG 1 cut(s) 26
NmuCI GTSAC 2 cut(s) 162, 690
NsiI ATGCAT 1 cut(s) 718
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 4 cut(s) 61, 122, 204, 592
PinAI ACCGGT 1 cut(s) 755
PkrI GCNGC 2 cut(s) 425, 428
PleI GAGTC 2 cut(s) 291, 617
PpsI GAGTC 2 cut(s) 291, 617
Psp124BI GAGCTC 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 754
PspPI GGNCC 1 cut(s) 753
PsuI RGATCY 2 cut(s) 458, 762
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 2 cut(s) 360, 666
SatI GCNGC 2 cut(s) 424, 427
Sau3AI GATC 3 cut(s) 458, 655, 762
Sau96I GGNCC 1 cut(s) 753
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 2 cut(s) 292, 618
ScrFI CCNGG 1 cut(s) 512
SduI GDGCHC 2 cut(s) 14, 542
SfaNI GCATC 4 cut(s) 292, 438, 484, 556
SfcI CTRYAG 1 cut(s) 245
SinI GGWCC 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
Sse9I AATT 5 cut(s) 258, 362, 488, 555, 660
SsiI CCGC 1 cut(s) 305
SspMI CTAG 3 cut(s) 72, 102, 183
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 510
TaiI ACGT 3 cut(s) 164, 338, 359
TaqI TCGA 4 cut(s) 64, 202, 588, 682
TasI AATT 5 cut(s) 258, 362, 488, 555, 660
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 4 cut(s) 61, 122, 204, 592
Tru1I TTAA 2 cut(s) 360, 666
Tru9I TTAA 2 cut(s) 360, 666
TscAI CASTG 1 cut(s) 624
TseFI GTSAC 2 cut(s) 162, 690
TseI GCWGC 2 cut(s) 423, 426
Tsp45I GTSAC 2 cut(s) 162, 690
TspDTI ATGAA 1 cut(s) 196
TspGWI ACGGA 2 cut(s) 217, 223
TspRI CASTG 1 cut(s) 624
VpaK11BI GGWCC 1 cut(s) 753
XapI RAATTY 3 cut(s) 362, 488, 660
XspI CTAG 3 cut(s) 72, 102, 183
ZrmI AGTACT 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.