MD07G1074500.v1.1

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008468
Physical Location & Seq
Forward (+)
7090926 .. 7095299
4374 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1074500.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCACTGAGCTCATTCTCAATTAAATTCGGTTCGATTTCGTGTCCCTACGAAAGCCACAGGACCGGTACGTCTCCAAGACTCCTCAAGCTCAACCATGTCCTGTCGGTTCGCGTTTCGGCCGATTCGACCCCAAAAGCCCGATTCGTCGCCCGCCGAACCGAGTCCGTCTCGGTTCCGCGGCTCCAGCGTCCTCTAAGTGAGTATATGAGCTTGCCGGCGAGTCAGTACTCGGTTTTGGACGCGGAGAGGATCGAGAGAGTGGATGCGAATACATTCAGGTGCTATGTGTATAGGTTCAAGTTCTTTGCGTTTGAGGTTTGCCCTGTTCTTCTTGTTAAAGTCGAAGAGCAGCCCAATGGCTGTAGCATTAAGCTCTTGTCCTGCAAGCTGGATGGTTCGCCGATTGCAGTTGCGCAGAATGACAAGTTTGACGCTTTTATGGTGAACCAAATATCGTGTGCTAGCAAAACCAACTCCTCACTACAAGAACTCAAATCAGATACCGTCATTGAGGTTAGCATTGATATTCCTTTTGCATTTCGTGCAATCCCAGTGCAAGCTATTGAATCATCTGGGACTCAAGTCCTTGAACAAATACTAAGAATTATGCTTCCCAGGTTTATGTCACAGCTTGTGAGGGATTATCGAGCCTGGGCTTCTGGTGATAGATCAAGGCAGCCTCTTGGAACTGGCGAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

235

Amino Acids

26.21

Weight (kDa)

9.08

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 65 - 220 2.8e-42 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 70
Acc16I TGCGCA 1 cut(s) 417
AccII CGCG 3 cut(s) 114, 181, 245
AciI CCGC 4 cut(s) 154, 179, 181, 245
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 1 cut(s) 26
AfaI GTAC 2 cut(s) 70, 230
AgeI ACCGGT 1 cut(s) 65
AgsI TTSAA 3 cut(s) 301, 569, 593
AjnI CCWGG 2 cut(s) 617, 653
AluBI AGCT 7 cut(s) 12, 91, 213, 376, 391, 563, 634
AluI AGCT 7 cut(s) 12, 91, 213, 376, 391, 563, 634
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 2 cut(s) 78, 175
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 2 cut(s) 352, 679
ApoI RAATTY 1 cut(s) 26
AsiGI ACCGGT 1 cut(s) 65
Asp700I GAANNNNTTC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 457, 677
AsuNHI GCTAGC 1 cut(s) 464
AvaII GGWCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 2 cut(s) 364, 691
BccI CCATC 1 cut(s) 389
BciT130I CCWGG 2 cut(s) 619, 655
BcoDI GTCTC 2 cut(s) 78, 175
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 364
BisI GCNGC 3 cut(s) 182, 353, 680
BlsI GCNGC 3 cut(s) 183, 354, 681
BmcAI AGTACT 1 cut(s) 230
Bme1390I CCNGG 2 cut(s) 619, 655
Bme18I GGWCC 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 2 cut(s) 177, 185
BmrFI CCNGG 2 cut(s) 619, 655
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 1 cut(s) 256
BmtI GCTAGC 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 548
BoxI GACNNNNGTC 1 cut(s) 584
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpmI CTGGAG 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 71, 567
BsaJI CCNNGG 3 cut(s) 179, 617, 654
BsaWI WCCGGW 1 cut(s) 65
Bse118I RCCGGY 2 cut(s) 65, 217
Bse1I ACTGG 2 cut(s) 554, 697
BseBI CCWGG 2 cut(s) 619, 655
BseDI CCNNGG 3 cut(s) 179, 617, 654
BseGI GGATG 2 cut(s) 271, 400
BseNI ACTGG 2 cut(s) 554, 697
BseRI GAGGAG 2 cut(s) 74, 469
BseX3I CGGCCG 1 cut(s) 120
BseXI GCAGC 2 cut(s) 364, 691
Bsh1236I CGCG 3 cut(s) 114, 181, 245
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 1 cut(s) 122
BshTI ACCGGT 1 cut(s) 65
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 2 cut(s) 66, 218
BslFI GGGAC 2 cut(s) 30, 592
BsmAI GTCTC 2 cut(s) 78, 175
BsmBI CGTCTC 2 cut(s) 78, 175
BsmFI GGGAC 2 cut(s) 30, 592
BsnI GGCC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 2 cut(s) 252, 671
BspACI CCGC 4 cut(s) 154, 179, 181, 245
BspANI GGCC 1 cut(s) 122
BspFNI CGCG 3 cut(s) 114, 181, 245
BspLI GGNNCC 2 cut(s) 177, 185
BspOI GCTAGC 1 cut(s) 468
BspPI GGATC 1 cut(s) 260
BspQI GCTCTTC 1 cut(s) 342
BsrFI RCCGGY 2 cut(s) 65, 217
BsrI ACTGG 2 cut(s) 554, 697
BssAI RCCGGY 2 cut(s) 65, 217
BssECI CCNNGG 3 cut(s) 179, 617, 654
BssMI GATC 2 cut(s) 252, 671
Bst2UI CCWGG 2 cut(s) 619, 655
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 6 cut(s) 154, 215, 219, 389, 466, 561
BstDEI CTNAG 3 cut(s) 8, 197, 602
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 271, 400
BstFNI CGCG 3 cut(s) 114, 181, 245
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 2 cut(s) 255, 674
BstMAI GTCTC 2 cut(s) 78, 175
BstMBI GATC 2 cut(s) 252, 671
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 2 cut(s) 187, 545
BstNI CCWGG 2 cut(s) 619, 655
BstPAI GACNNNNGTC 1 cut(s) 584
BstSCI CCNGG 2 cut(s) 617, 653
BstSFI CTRYAG 1 cut(s) 364
BstUI CGCG 3 cut(s) 114, 181, 245
BstV1I GCAGC 2 cut(s) 364, 691
BstZI CGGCCG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 271, 400
BtsIMutI CAGTG 2 cut(s) 5, 561
Cac8I GCNNGC 6 cut(s) 154, 215, 219, 389, 466, 561
CfoI GCGC 1 cut(s) 418
Cfr10I RCCGGY 2 cut(s) 65, 217
Cfr13I GGNCC 1 cut(s) 63
Cfr42I CCGCGG 1 cut(s) 182
CseI GACGC 3 cut(s) 179, 251, 443
Csp6I GTAC 2 cut(s) 69, 229
CspAI ACCGGT 1 cut(s) 65
CviAII CATG 1 cut(s) 98
CviQI GTAC 2 cut(s) 69, 229
DdeI CTNAG 3 cut(s) 8, 197, 602
DpnI GATC 2 cut(s) 254, 673
DpnII GATC 2 cut(s) 252, 671
DrdI GACNNNNNNGTC 1 cut(s) 70
DseDI GACNNNNNNGTC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 120
EagI CGGCCG 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 12
EclXI CGGCCG 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 63
Eco52I CGGCCG 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 12
EcoICRI GAGCTC 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 617, 653
EcoT38I GRGCYC 1 cut(s) 14
Esp3I CGTCTC 2 cut(s) 78, 175
FaeI CATG 1 cut(s) 101
FaiI YATR 9 cut(s) 99, 207, 209, 288, 294, 443, 611, 626, 703
FaqI GGGAC 2 cut(s) 30, 592
FatI CATG 1 cut(s) 97
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 182, 353, 680
FokI GGATG 2 cut(s) 278, 407
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 3 cut(s) 182, 353, 680
FspBI CTAG 1 cut(s) 465
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 1 cut(s) 417
GluI GCNGC 3 cut(s) 182, 353, 680
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 1 cut(s) 122
HapII CCGG 2 cut(s) 66, 218
HgaI GACGC 3 cut(s) 179, 251, 443
HhaI GCGC 1 cut(s) 418
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HinfI GANTC 7 cut(s) 81, 125, 144, 164, 223, 569, 580
HpaII CCGG 2 cut(s) 66, 218
HphI GGTGA 2 cut(s) 457, 677
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 502
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 5 cut(s) 387, 410, 539, 548, 559
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 545
HpyF3I CTNAG 3 cut(s) 8, 197, 602
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 1 cut(s) 416
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
KspI CCGCGG 1 cut(s) 182
Kzo9I GATC 2 cut(s) 252, 671
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 189
Lsp1109I GCAGC 2 cut(s) 364, 691
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 1 cut(s) 627
MalI GATC 2 cut(s) 254, 673
MboI GATC 2 cut(s) 252, 671
MboII GAAGA 2 cut(s) 323, 359
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 3 cut(s) 21, 26, 606
MlyI GAGTC 4 cut(s) 75, 173, 232, 574
MnlI CCTC 8 cut(s) 95, 204, 243, 310, 490, 508, 633, 693
MroNI GCCGGC 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 3 cut(s) 24, 339, 372
MslI CAYNNNNRTG 1 cut(s) 280
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 2 cut(s) 66, 218
MspR9I CCNGG 2 cut(s) 619, 655
MvaI CCWGG 2 cut(s) 619, 655
MvnI CGCG 3 cut(s) 114, 181, 245
MwoI GCNNNNNNNGC 2 cut(s) 187, 545
NaeI GCCGGC 1 cut(s) 219
NdeII GATC 2 cut(s) 252, 671
NgoMIV GCCGGC 1 cut(s) 217
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 2 cut(s) 177, 185
NmuCI GTSAC 1 cut(s) 627
NsbI TGCGCA 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PdiI GCCGGC 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 3 cut(s) 125, 144, 569
PinAI ACCGGT 1 cut(s) 65
PkrI GCNGC 3 cut(s) 183, 354, 681
PleI GAGTC 4 cut(s) 75, 172, 231, 574
PpsI GAGTC 4 cut(s) 75, 172, 231, 574
PshAI GACNNNNGTC 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 2 cut(s) 617, 653
PspGI CCWGG 2 cut(s) 617, 653
PspN4I GGNNCC 2 cut(s) 177, 185
PspPI GGNCC 1 cut(s) 63
RsaI GTAC 2 cut(s) 70, 230
RsaNI GTAC 2 cut(s) 69, 229
RseI CAYNNNNRTG 1 cut(s) 280
SacI GAGCTC 1 cut(s) 14
SacII CCGCGG 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 3 cut(s) 24, 339, 372
SatI GCNGC 3 cut(s) 182, 353, 680
Sau3AI GATC 2 cut(s) 252, 671
Sau96I GGNCC 1 cut(s) 63
ScaI AGTACT 1 cut(s) 230
SchI GAGTC 4 cut(s) 75, 173, 232, 574
ScrFI CCNGG 2 cut(s) 619, 655
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 364
Sfr303I CCGCGG 1 cut(s) 182
SgrBI CCGCGG 1 cut(s) 182
SinI GGWCC 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 86, 582
SmoI CTYRAG 2 cut(s) 86, 582
Sse9I AATT 3 cut(s) 21, 26, 606
SsiI CCGC 4 cut(s) 154, 179, 181, 245
SspMI CTAG 1 cut(s) 465
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 617, 653
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 74
TaqI TCGA 5 cut(s) 35, 128, 255, 345, 649
TasI AATT 3 cut(s) 21, 26, 606
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 184
TfiI GAWTC 3 cut(s) 125, 144, 569
Tru1I TTAA 3 cut(s) 24, 339, 372
Tru9I TTAA 3 cut(s) 24, 339, 372
TscAI CASTG 2 cut(s) 12, 561
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 2 cut(s) 352, 679
Tsp45I GTSAC 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 2 cut(s) 12, 561
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 26
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 1 cut(s) 465
ZrmI AGTACT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.