RLG00000029378

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008468
Physical Location & Seq
Reverse (-)
37431744 .. 37437739
5996 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029378

Sequence Viewer

Length: 717 bp
ATGGCAGTAAGCTCACTCTCCATAAACTTGTGGCATCCCTTCGAATACTCTCGCACCGGAAGGTCTCCACGGCTCAAACTCAAGCATGTCCTCTTCGCTCCCACGAAACGCAGCCTTCGCGTTTCGGCCGTTTCGACGCCGCCCAAAGCCCGTTTCGTCGCCCGTCGGACCGAGTCCGTCCCGGTTCCGCAGCTCGAACGGCCTCTAAGTGAATATATGAGCTTGCCGGCGAGTCAGTACTCGGTTTTGGACGCGGAGAGGATAGAGAGAGTGGACGACAACACGTTCAGGTGCTATGTGTATAGATTCAAGTTCTTTGCTTTCGAGGTCTGCCCTGTTCTTCTTGTTAGAGTCGAAGAGCAGCCTAATGGCTGTTGCATTAAGCTCTTGTCCTGCAAGCTTGAGGGGTCACCCATTGTGGTTGCGCAGAATGACAAGTTTGACGCTTATATGGTGAACCAAATATCATGTGGAAGCAACAAAAGCAGTTCATCACTGCAACAACTCAGTTCAGAAACTGTCATTGAGGTCAGTATTGAGATTCCGTTTGCATTTCGTGCAATTCCAGCGCAAGCTATTGAATCTTCTGGGACCCAAGTCCTTGAACAAATACTAAGAATTATGCTTCCTCGATTTTTAGCGCAGCTTGTGAAGGACTATCGAGCATGGGCCTCTGGTGATAGATCAAGACAGCCTCTTGGAACTGGCGAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

239

Amino Acids

26.84

Weight (kDa)

9.2

Isoelectric Point (pI)

66.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 68 - 224 1.9e-43 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 426
AccII CGCG 2 cut(s) 120, 254
AciI CCGC 3 cut(s) 140, 188, 254
AcoI YGGCCR 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 3 cut(s) 310, 581, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 7 cut(s) 12, 193, 222, 385, 400, 575, 646
AluI AGCT 7 cut(s) 12, 193, 222, 385, 400, 575, 646
Alw26I GTCTC 1 cut(s) 69
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 3 cut(s) 126, 200, 669
ApeKI GCWGC 4 cut(s) 111, 190, 361, 643
AspLEI GCGC 3 cut(s) 427, 571, 643
AspS9I GGNCC 3 cut(s) 168, 591, 669
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 402, 466, 689
AsuII TTCGAA 1 cut(s) 42
AvaII GGWCC 2 cut(s) 168, 591
BbvI GCAGC 4 cut(s) 123, 202, 373, 655
BceAI ACGGC 3 cut(s) 86, 113, 215
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 1 cut(s) 69
BisI GCNGC 5 cut(s) 112, 140, 191, 362, 644
BlsI GCNGC 5 cut(s) 113, 141, 192, 363, 645
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 2 cut(s) 168, 591
BmgT120I GGNCC 3 cut(s) 168, 591, 669
BmiI GGNNCC 3 cut(s) 186, 592, 593
BmrFI CCNGG 1 cut(s) 182
BmsI GCATC 1 cut(s) 43
BoxI GACNNNNGTC 1 cut(s) 596
Bpu14I TTCGAA 1 cut(s) 42
BpuEI CTTGAG 2 cut(s) 65, 422
BpuMI CCSGG 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 56
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 709
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 520
BseNI ACTGG 1 cut(s) 709
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 4 cut(s) 123, 202, 373, 655
Bsh1236I CGCG 2 cut(s) 120, 254
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 3 cut(s) 128, 202, 671
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 3 cut(s) 57, 182, 227
BslFI GGGAC 2 cut(s) 164, 604
BsmAI GTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 164, 604
BsnI GGCC 3 cut(s) 128, 202, 671
Bso31I GGTCTC 1 cut(s) 69
Bsp119I TTCGAA 1 cut(s) 42
Bsp143I GATC 1 cut(s) 683
BspACI CCGC 3 cut(s) 140, 188, 254
BspANI GGCC 3 cut(s) 128, 202, 671
BspCNI CTCAG 1 cut(s) 519
BspFNI CGCG 2 cut(s) 120, 254
BspLI GGNNCC 3 cut(s) 186, 592, 593
BspQI GCTCTTC 1 cut(s) 351
BspT104I TTCGAA 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 709
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 1 cut(s) 683
BssNI GRCGYC 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 2 cut(s) 98, 351
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 4 cut(s) 224, 228, 398, 573
BstDEI CTNAG 3 cut(s) 206, 506, 614
BstDSI CCRYGG 1 cut(s) 68
BstEII GGTNACC 1 cut(s) 408
BstF5I GGATG 1 cut(s) 34
BstFNI CGCG 2 cut(s) 120, 254
BstHHI GCGC 3 cut(s) 427, 571, 643
BstKTI GATC 1 cut(s) 686
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 1 cut(s) 683
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 5 cut(s) 117, 199, 483, 557, 566
BstNSI RCATGY 1 cut(s) 89
BstPAI GACNNNNGTC 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 180
BstUI CGCG 2 cut(s) 120, 254
BstV1I GCAGC 4 cut(s) 123, 202, 373, 655
BstZI CGGCCG 1 cut(s) 126
BsuRI GGCC 3 cut(s) 128, 202, 671
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 1 cut(s) 34
BtsI GCAGTG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 4 cut(s) 224, 228, 398, 573
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 3 cut(s) 427, 571, 643
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 3 cut(s) 168, 591, 669
CpoI CGGWCCG 1 cut(s) 168
CseI GACGC 3 cut(s) 145, 260, 452
Csp6I GTAC 1 cut(s) 238
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 3 cut(s) 86, 468, 666
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 3 cut(s) 206, 506, 614
DpnI GATC 1 cut(s) 685
DpnII GATC 1 cut(s) 683
EaeI YGGCCR 1 cut(s) 126
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 2 cut(s) 98, 351
EarI CTCTTC 2 cut(s) 98, 351
EclXI CGGCCG 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 168, 591
Eco52I CGGCCG 1 cut(s) 126
Eco91I GGTNACC 1 cut(s) 408
EcoO109I RGGNCCY 1 cut(s) 591
EcoO65I GGTNACC 1 cut(s) 408
FaeI CATG 3 cut(s) 89, 471, 669
FaqI GGGAC 2 cut(s) 164, 604
FatI CATG 3 cut(s) 85, 467, 665
Fnu4HI GCNGC 5 cut(s) 112, 140, 191, 362, 644
FokI GGATG 1 cut(s) 21
Fsp4HI GCNGC 5 cut(s) 112, 140, 191, 362, 644
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 3 cut(s) 426, 570, 642
GluI GCNGC 5 cut(s) 112, 140, 191, 362, 644
HaeIII GGCC 3 cut(s) 128, 202, 671
HapII CCGG 3 cut(s) 57, 182, 227
HgaI GACGC 3 cut(s) 145, 260, 452
HhaI GCGC 3 cut(s) 427, 571, 643
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 3 cut(s) 89, 471, 669
Hin6I GCGC 3 cut(s) 425, 569, 641
HinP1I GCGC 3 cut(s) 425, 569, 641
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 6 cut(s) 173, 232, 306, 351, 541, 581
HpaII CCGG 3 cut(s) 57, 182, 227
HphI GGTGA 3 cut(s) 402, 466, 689
Hpy166II GTNNAC 2 cut(s) 274, 457
Hpy188I TCNGA 2 cut(s) 168, 514
Hpy188III TCNNGA 1 cut(s) 687
Hpy8I GTNNAC 2 cut(s) 274, 457
Hpy99I CGWCG 3 cut(s) 139, 161, 168
HpyAV CCTTC 4 cut(s) 49, 54, 125, 646
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4IV ACGT 1 cut(s) 284
HpyCH4V TGCA 5 cut(s) 378, 396, 499, 551, 560
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 199, 483, 557, 566
HpyF3I CTNAG 3 cut(s) 206, 506, 614
HpySE526I ACGT 1 cut(s) 284
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 3 cut(s) 89, 471, 669
HspAI GCGC 3 cut(s) 425, 569, 641
KflI GGGWCCC 1 cut(s) 591
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Kzo9I GATC 1 cut(s) 683
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 4 cut(s) 123, 202, 373, 655
LweI GCATC 1 cut(s) 43
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 408
MalI GATC 1 cut(s) 685
MboI GATC 1 cut(s) 683
MboII GAAGA 4 cut(s) 85, 332, 368, 576
MluCI AATT 2 cut(s) 561, 618
MlyI GAGTC 3 cut(s) 182, 241, 360
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 9 cut(s) 101, 213, 252, 319, 397, 520, 639, 682, 705
MroNI GCCGGC 1 cut(s) 226
MseI TTAA 1 cut(s) 381
MspI CCGG 3 cut(s) 57, 182, 227
MspR9I CCNGG 1 cut(s) 182
MvnI CGCG 2 cut(s) 120, 254
MwoI GCNNNNNNNGC 5 cut(s) 117, 199, 483, 557, 566
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 1 cut(s) 182
NdeII GATC 1 cut(s) 683
NgoMIV GCCGGC 1 cut(s) 226
NlaIII CATG 3 cut(s) 89, 471, 669
NlaIV GGNNCC 3 cut(s) 186, 592, 593
NmuCI GTSAC 1 cut(s) 408
NsbI TGCGCA 1 cut(s) 426
NspI RCATGY 1 cut(s) 89
NspV TTCGAA 1 cut(s) 42
PciSI GCTCTTC 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 3 cut(s) 306, 541, 581
PflFI GACNNNGTC 1 cut(s) 172
PkrI GCNGC 5 cut(s) 113, 141, 192, 363, 645
PleI GAGTC 3 cut(s) 181, 240, 359
PpsI GAGTC 3 cut(s) 181, 240, 359
PpuMI RGGWCCY 1 cut(s) 591
PshAI GACNNNNGTC 1 cut(s) 596
Psp5II RGGWCCY 1 cut(s) 591
PspEI GGTNACC 1 cut(s) 408
PspN4I GGNNCC 3 cut(s) 186, 592, 593
PspPI GGNCC 3 cut(s) 168, 591, 669
PspPPI RGGWCCY 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 518
PsyI GACNNNGTC 1 cut(s) 172
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 5 cut(s) 112, 140, 191, 362, 644
Sau3AI GATC 1 cut(s) 683
Sau96I GGNCC 3 cut(s) 168, 591, 669
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 3 cut(s) 182, 241, 360
ScrFI CCNGG 1 cut(s) 182
SfaNI GCATC 1 cut(s) 43
SfuI TTCGAA 1 cut(s) 42
SinI GGWCC 2 cut(s) 168, 591
SmlI CTYRAG 2 cut(s) 80, 401
SmoI CTYRAG 2 cut(s) 80, 401
Sse9I AATT 2 cut(s) 561, 618
SsiI CCGC 3 cut(s) 140, 188, 254
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 520
TaiI ACGT 1 cut(s) 287
TaqI TCGA 7 cut(s) 42, 134, 195, 324, 354, 631, 661
TaqII GACCGA 1 cut(s) 185
TasI AATT 2 cut(s) 561, 618
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 3 cut(s) 306, 541, 581
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 1 cut(s) 501
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 4 cut(s) 111, 190, 361, 643
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 1 cut(s) 480
TspGWI ACGGA 2 cut(s) 166, 534
TspRI CASTG 1 cut(s) 501
Tth111I GACNNNGTC 1 cut(s) 172
VpaK11BI GGWCC 2 cut(s) 168, 591
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.