FvH4_7g06650

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008468
Physical Location & Seq
Forward (+)
6690777 .. 6697498
6722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06650.t7

Sequence Viewer

Length: 426 bp
ATGGCGGCGACCTCATTCTACCAACACTCCCCCGGACTCAAACTCAAGCATGTCCTCTTCGCTCTCGCTCCCCCGAATCCTCGCCTTCGCGTTTCGGCCGTTTCGACACCGCCCAAGGCCCGTTTCGTCGCCCGGCGTACCGAGTCCGTCCCGGTCCCGCAGCTCCAACGGCCCCTAAGTGAATATATGAGCTTGCCGGCGAGTCAGTACTCGGTGTTGGACGCGGAGAGGATAGAGAGAGTGGACGACAACACGTTCAGGTGCTATGTGTATAGGTTCAAGTTCTTTGCTTTCGAGGTCTGCCCTGTTCTTCTCGTTAAAGTCGAAGAGCAGCCCAATGGTTGTTGCATTAAGCTCTTGTCCTGCAAGCTTGACGGGTCCCCGATTGTGGTTGCGCAGAATGACAAGTTTGACGGAGGTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

142

Amino Acids

15.83

Weight (kDa)

9.06

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 58 - 124 2.3e-17 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 396
AccII CGCG 2 cut(s) 90, 224
AciI CCGC 4 cut(s) 5, 110, 158, 224
AcoI YGGCCR 1 cut(s) 96
AfaI GTAC 2 cut(s) 139, 209
AfiI CCNNNNNNNGG 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 1 cut(s) 280
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 4 cut(s) 163, 192, 355, 370
AluI AGCT 4 cut(s) 163, 192, 355, 370
AoxI GGCC 3 cut(s) 96, 117, 170
ApeKI GCWGC 2 cut(s) 160, 331
AspLEI GCGC 1 cut(s) 397
AspS9I GGNCC 4 cut(s) 118, 154, 171, 378
AsuC2I CCSGG 3 cut(s) 33, 133, 152
AvaII GGWCC 2 cut(s) 154, 378
BbvI GCAGC 2 cut(s) 172, 343
BceAI ACGGC 2 cut(s) 83, 185
BcnI CCSGG 3 cut(s) 33, 133, 152
BisI GCNGC 3 cut(s) 6, 161, 332
BlsI GCNGC 3 cut(s) 7, 162, 333
BmcAI AGTACT 1 cut(s) 209
Bme1390I CCNGG 3 cut(s) 33, 133, 152
Bme18I GGWCC 2 cut(s) 154, 378
BmgT120I GGNCC 4 cut(s) 118, 154, 171, 378
BmiI GGNNCC 4 cut(s) 156, 173, 379, 380
BmrFI CCNGG 3 cut(s) 33, 133, 152
BoxI GACNNNNGTC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 3 cut(s) 33, 133, 152
BsaJI CCNNGG 2 cut(s) 31, 114
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 31, 114
BseLI CCNNNNNNNGG 1 cut(s) 388
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 2 cut(s) 172, 343
Bsh1236I CGCG 2 cut(s) 90, 224
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 98, 119, 172
BsiEI CGRYCG 1 cut(s) 99
BsiSI CCGG 4 cut(s) 33, 133, 152, 197
BslFI GGGAC 3 cut(s) 134, 140, 364
BslI CCNNNNNNNGG 1 cut(s) 388
BsmFI GGGAC 3 cut(s) 134, 140, 364
BsnI GGCC 3 cut(s) 98, 119, 172
BspACI CCGC 4 cut(s) 5, 110, 158, 224
BspANI GGCC 3 cut(s) 98, 119, 172
BspFNI CGCG 2 cut(s) 90, 224
BspLI GGNNCC 4 cut(s) 156, 173, 379, 380
BspQI GCTCTTC 1 cut(s) 321
BsrFI RCCGGY 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 31, 114
BssT1I CCWWGG 1 cut(s) 114
Bst6I CTCTTC 2 cut(s) 62, 321
BstC8I GCNNGC 3 cut(s) 194, 198, 368
BstDEI CTNAG 1 cut(s) 176
BstFNI CGCG 2 cut(s) 90, 224
BstHHI GCGC 1 cut(s) 397
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 53
BstPAI GACNNNNGTC 1 cut(s) 417
BstSCI CCNGG 3 cut(s) 31, 131, 150
BstUI CGCG 2 cut(s) 90, 224
BstV1I GCAGC 2 cut(s) 172, 343
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 3 cut(s) 98, 119, 172
Cac8I GCNNGC 3 cut(s) 194, 198, 368
CfoI GCGC 1 cut(s) 397
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 4 cut(s) 118, 154, 171, 378
CseI GACGC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 138, 208
CviAII CATG 1 cut(s) 50
CviJI RGCY 8 cut(s) 98, 119, 163, 172, 192, 334, 355, 370
CviKI_1 RGCY 8 cut(s) 98, 119, 163, 172, 192, 334, 355, 370
CviQI GTAC 2 cut(s) 138, 208
DdeI CTNAG 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 62, 321
EarI CTCTTC 2 cut(s) 62, 321
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 154, 378
Eco52I CGGCCG 1 cut(s) 96
EcoO109I RGGNCCY 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 1 cut(s) 53
FaiI YATR 5 cut(s) 51, 186, 188, 267, 273
FaqI GGGAC 3 cut(s) 134, 140, 364
FatI CATG 1 cut(s) 49
FauI CCCGC 1 cut(s) 165
Fnu4HI GCNGC 3 cut(s) 6, 161, 332
Fsp4HI GCNGC 3 cut(s) 6, 161, 332
FspI TGCGCA 1 cut(s) 396
GlaI GCGC 1 cut(s) 396
GluI GCNGC 3 cut(s) 6, 161, 332
HaeIII GGCC 3 cut(s) 98, 119, 172
HapII CCGG 4 cut(s) 33, 133, 152, 197
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 397
Hin1II CATG 1 cut(s) 53
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 4 cut(s) 36, 76, 143, 202
HpaII CCGG 4 cut(s) 33, 133, 152, 197
Hpy166II GTNNAC 1 cut(s) 244
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 2 cut(s) 348, 366
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 1 cut(s) 176
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 1 cut(s) 53
HspAI GCGC 1 cut(s) 395
KflI GGGWCCC 1 cut(s) 378
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 73, 168
LpnPI CCDG 7 cut(s) 46, 146, 165, 210, 244, 318, 376
Lsp1109I GCAGC 2 cut(s) 172, 343
MaeII ACGT 1 cut(s) 254
MboII GAAGA 3 cut(s) 49, 302, 338
MlyI GAGTC 3 cut(s) 30, 152, 211
MmeI TCCRAC 2 cut(s) 190, 198
MnlI CCTC 6 cut(s) 22, 65, 90, 222, 289, 410
MroNI GCCGGC 1 cut(s) 196
MseI TTAA 2 cut(s) 318, 351
MspI CCGG 4 cut(s) 33, 133, 152, 197
MspR9I CCNGG 3 cut(s) 33, 133, 152
MvnI CGCG 2 cut(s) 90, 224
MwoI GCNNNNNNNGC 1 cut(s) 169
NaeI GCCGGC 1 cut(s) 198
NciI CCSGG 3 cut(s) 33, 133, 152
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 1 cut(s) 53
NlaIV GGNNCC 4 cut(s) 156, 173, 379, 380
NsbI TGCGCA 1 cut(s) 396
NspI RCATGY 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 2 cut(s) 101, 321
PdiI GCCGGC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 3 cut(s) 7, 162, 333
PleI GAGTC 3 cut(s) 30, 151, 210
PpsI GAGTC 3 cut(s) 30, 151, 210
PpuMI RGGWCCY 1 cut(s) 378
PshAI GACNNNNGTC 1 cut(s) 417
Psp5II RGGWCCY 1 cut(s) 378
PspN4I GGNNCC 4 cut(s) 156, 173, 379, 380
PspPI GGNCC 4 cut(s) 118, 154, 171, 378
PspPPI RGGWCCY 1 cut(s) 378
RsaI GTAC 2 cut(s) 139, 209
RsaNI GTAC 2 cut(s) 138, 208
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 2 cut(s) 318, 351
SatI GCNGC 3 cut(s) 6, 161, 332
Sau96I GGNCC 4 cut(s) 118, 154, 171, 378
ScaI AGTACT 1 cut(s) 209
SchI GAGTC 3 cut(s) 30, 152, 211
ScrFI CCNGG 3 cut(s) 33, 133, 152
SinI GGWCC 2 cut(s) 154, 378
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SsiI CCGC 4 cut(s) 5, 110, 158, 224
StyD4I CCNGG 3 cut(s) 31, 131, 150
StyI CCWWGG 1 cut(s) 114
TaiI ACGT 1 cut(s) 257
TaqI TCGA 3 cut(s) 104, 294, 324
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 2 cut(s) 318, 351
Tru9I TTAA 2 cut(s) 318, 351
TseI GCWGC 2 cut(s) 160, 331
TspGWI ACGGA 1 cut(s) 136
VpaK11BI GGWCC 2 cut(s) 154, 378
XceI RCATGY 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.