AT5G05250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015947
Physical Location & Seq
Reverse (-)
1556987 .. 1558486
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05250.1

Sequence Viewer

Length: 720 bp
ATGGCGTATGCAAAGATCGGTTACGGTGGAAGTTATCTAGTGCAACGCGGCATATTGGAGTATCCGTGTGGATCCGATCATAATGCGAGATTGATTTCATCGTCGTTTCCGATTAATCTAGGCGGTAATCGACGATCTAGCATCGGAACAATCTCGGCGTCGATAGGGGAGGCTGTGACTGAGATAGAGAAACAGAGAGGTGGAAGAAGAAGCGGAGGGAGAAGCAGAGGAGATCGTTGGGAGGTGGAAGCGGAGCGTGAGAAGCTAGACAGGTGGATGAAGGAATCGGTGACGGAGATCGTTAAGAATTTAAGTGAAGCGCCGTTGTTAGTCCATTTGTACACCGGAGATAAGGAGGAGGGAACGGTAGTTGTGATGAAAGCAGAGGAATGGGCGGCGGTGAAGGGGAGGTGGGAGAGAGGAGAGGCGGAGATGCCGGAAGGGATAGTGTTCGTGGAGCAGTTAGGAGCGGCGGAGGAGAGTTGTGGCTGTGGTTTTGACGGTGGAGACGGCACACGTGCGTGGGGGTTGGTGGTGCAAGGGAGAGGAGTTGAGTGTGGGCCGGTTTGTTATCTGCTTAAGACGACCCGTGTAGGGTCAGGGTCAGGATCCGGGTCGGGTTTGGGTATGCGGTGTACGCATTTCTGTTTAGCTAAGGTGTCGAGTTTTAGAGAGACTAGTGAATCTCAGCTTAGGAATTGTTGGCTTGTGGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

239

Amino Acids

25.86

Weight (kDa)

6.36

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7804 PF25089 85 - 160 4.8e-28 Domain of unknown function (DUF7804)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56360 AT5G05250
fragaria_vesca FvH4_7g25910
malus_domestica MD01G1167600.v1.1 MD07G1233600.v1.1
prunus_persica Prupe.2G264000_v2.0.a1
pyrus_communis pycom01g18060 pycom07g21140
rosa_chinensis RchiOBHm_Chr1g0372231
rosa_rugosa Rorug01G0370600
rosa_samantha Rh1AG378900 Rh1BG342400 Rh1CG356000 Rh1DG373600
rosa_wichuraiana Rw1G033340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 470
AccII CGCG 1 cut(s) 48
AclWI GGATC 4 cut(s) 66, 79, 603, 616
AcsI RAATTY 1 cut(s) 307
AcvI CACGTG 1 cut(s) 518
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 2 cut(s) 341, 637
AfiI CCNNNNNNNGG 1 cut(s) 594
AflII CTTAAG 1 cut(s) 578
AflIII ACRYGT 1 cut(s) 515
AhlI ACTAGT 1 cut(s) 677
AleI CACNNNNGTG 1 cut(s) 520
AluBI AGCT 3 cut(s) 265, 653, 691
AluI AGCT 3 cut(s) 265, 653, 691
Alw26I GTCTC 2 cut(s) 501, 668
AlwI GGATC 4 cut(s) 66, 79, 603, 616
AoxI GGCC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 307
AseI ATTAAT 1 cut(s) 114
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 560
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 301, 412
BamHI GGATCC 2 cut(s) 71, 608
BbrPI CACGTG 1 cut(s) 518
BceAI ACGGC 2 cut(s) 307, 526
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciVI GTATCC 1 cut(s) 72
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 2 cut(s) 501, 668
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 5 cut(s) 38, 119, 138, 266, 678
BfoI RGCGCY 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 578
BfuI GTATCC 1 cut(s) 72
BisI GCNGC 3 cut(s) 49, 396, 471
BlsI GCNGC 3 cut(s) 50, 397, 472
Bme1390I CCNGG 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 2 cut(s) 73, 610
BmrFI CCNGG 1 cut(s) 613
BmsI GCATC 2 cut(s) 150, 423
Bpu10I CCTNAGC 2 cut(s) 654, 692
BpuMI CCSGG 1 cut(s) 613
BsaAI YACGTR 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 158
BsaWI WCCGGW 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse118I RCCGGY 1 cut(s) 562
BseGI GGATG 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMII CTCAG 2 cut(s) 171, 701
BseRI GAGGAG 5 cut(s) 243, 371, 435, 491, 561
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 562
BsiSI CCGG 4 cut(s) 345, 437, 563, 612
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 2 cut(s) 501, 668
BsmBI CGTCTC 1 cut(s) 501
BsnI GGCC 1 cut(s) 562
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
BspANI GGCC 1 cut(s) 562
BspCNI CTCAG 2 cut(s) 172, 700
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 2 cut(s) 73, 610
BspPI GGATC 4 cut(s) 66, 79, 603, 616
BspTI CTTAAG 1 cut(s) 578
BsrBI CCGCTC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 562
BsrGI TGTACA 1 cut(s) 339
BssAI RCCGGY 1 cut(s) 562
BssMI GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
BssNI GRCGYC 1 cut(s) 158
Bst4CI ACNGT 3 cut(s) 26, 367, 503
BstACI GRCGYC 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 518
BstDEI CTNAG 4 cut(s) 180, 654, 687, 692
BstF5I GGATG 1 cut(s) 282
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 7 cut(s) 18, 74, 79, 137, 235, 300, 611
BstMAI GTCTC 2 cut(s) 501, 668
BstMBI GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
BstMWI GCNNNNNNNGC 2 cut(s) 262, 637
BstSCI CCNGG 1 cut(s) 611
BstUI CGCG 1 cut(s) 48
BstX2I RGATCY 2 cut(s) 71, 608
BstYI RGATCY 2 cut(s) 71, 608
BsuI GTATCC 1 cut(s) 72
BsuRI GGCC 1 cut(s) 562
BtsCI GGATG 1 cut(s) 282
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 1 cut(s) 560
CseI GACGC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 340, 636
CviJI RGCY 7 cut(s) 173, 265, 489, 562, 653, 691, 706
CviKI_1 RGCY 7 cut(s) 173, 265, 489, 562, 653, 691, 706
CviQI GTAC 2 cut(s) 340, 636
DdeI CTNAG 4 cut(s) 180, 654, 687, 692
DpnI GATC 7 cut(s) 17, 73, 78, 136, 234, 299, 610
DpnII GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
EciI GGCGGA 2 cut(s) 443, 488
Eco72I CACGTG 1 cut(s) 518
Esp3I CGTCTC 1 cut(s) 501
FaiI YATR 4 cut(s) 9, 53, 81, 629
Fnu4HI GCNGC 3 cut(s) 49, 396, 471
FokI GGATG 1 cut(s) 289
Fsp4HI GCNGC 3 cut(s) 49, 396, 471
FspBI CTAG 5 cut(s) 38, 119, 138, 266, 678
GlaI GCGC 1 cut(s) 321
GluI GCNGC 3 cut(s) 49, 396, 471
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 1 cut(s) 562
HapII CCGG 4 cut(s) 345, 437, 563, 612
HgaI GACGC 1 cut(s) 147
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 158
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 2 cut(s) 284, 683
HpaII CCGG 4 cut(s) 345, 437, 563, 612
HphI GGTGA 2 cut(s) 301, 412
Hpy166II GTNNAC 2 cut(s) 342, 636
Hpy188I TCNGA 3 cut(s) 76, 111, 146
Hpy188III TCNNGA 1 cut(s) 606
Hpy8I GTNNAC 2 cut(s) 342, 636
Hpy99I CGWCG 3 cut(s) 106, 135, 163
HpyAV CCTTC 3 cut(s) 274, 397, 434
HpyCH4III ACNGT 3 cut(s) 26, 367, 503
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 3 cut(s) 11, 43, 538
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 637
HpyF3I CTNAG 4 cut(s) 180, 654, 687, 692
HpySE526I ACGT 1 cut(s) 517
Hsp92I GRCGYC 1 cut(s) 158
HspAI GCGC 1 cut(s) 320
Kzo9I GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
LmnI GCTCC 3 cut(s) 253, 457, 467
LpnPI CCDG 7 cut(s) 256, 358, 450, 576, 585, 591, 625
LweI GCATC 2 cut(s) 150, 423
MaeI CTAG 5 cut(s) 38, 119, 138, 266, 678
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 3 cut(s) 20, 175, 289
MalI GATC 7 cut(s) 17, 73, 78, 136, 234, 299, 610
MbiI CCGCTC 1 cut(s) 470
MboI GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
MboII GAAGA 2 cut(s) 216, 219
MflI RGATCY 2 cut(s) 71, 608
MluCI AATT 3 cut(s) 307, 697, 715
MseI TTAA 4 cut(s) 114, 303, 311, 579
MslI CAYNNNNRTG 1 cut(s) 520
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 4 cut(s) 345, 437, 563, 612
MspR9I CCNGG 1 cut(s) 613
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 262, 637
NciI CCSGG 1 cut(s) 613
NdeII GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
NlaIV GGNNCC 2 cut(s) 73, 610
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 2 cut(s) 175, 289
OliI CACNNNNGTG 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PfeI GAWTC 2 cut(s) 284, 683
PkrI GCNGC 3 cut(s) 50, 397, 472
PmaCI CACGTG 1 cut(s) 518
PmlI CACGTG 1 cut(s) 518
Ppu21I YACGTR 1 cut(s) 518
PshBI ATTAAT 1 cut(s) 114
PspCI CACGTG 1 cut(s) 518
PspN4I GGNNCC 2 cut(s) 73, 610
PspPI GGNCC 1 cut(s) 560
PsuI RGATCY 2 cut(s) 71, 608
RsaI GTAC 2 cut(s) 341, 637
RsaNI GTAC 2 cut(s) 340, 636
RseI CAYNNNNRTG 1 cut(s) 520
SaqAI TTAA 4 cut(s) 114, 303, 311, 579
SatI GCNGC 3 cut(s) 49, 396, 471
Sau3AI GATC 7 cut(s) 15, 71, 76, 134, 232, 297, 608
Sau96I GGNCC 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 613
SetI ASST 9 cut(s) 202, 246, 267, 275, 413, 520, 655, 660, 693
SfaNI GCATC 2 cut(s) 150, 423
SmiMI CAYNNNNRTG 1 cut(s) 520
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SpeI ACTAGT 1 cut(s) 677
Sse9I AATT 3 cut(s) 307, 697, 715
SspMI CTAG 5 cut(s) 38, 119, 138, 266, 678
StyD4I CCNGG 1 cut(s) 611
TaaI ACNGT 3 cut(s) 26, 367, 503
TaiI ACGT 1 cut(s) 520
TaqI TCGA 3 cut(s) 130, 161, 662
TasI AATT 3 cut(s) 307, 697, 715
TatI WGTACW 1 cut(s) 339
TauI GCSGC 3 cut(s) 51, 398, 473
TfiI GAWTC 2 cut(s) 284, 683
Tru1I TTAA 4 cut(s) 114, 303, 311, 579
Tru9I TTAA 4 cut(s) 114, 303, 311, 579
TseFI GTSAC 2 cut(s) 175, 289
Tsp45I GTSAC 2 cut(s) 175, 289
TspDTI ATGAA 3 cut(s) 87, 293, 392
TspGWI ACGGA 2 cut(s) 54, 308
Vha464I CTTAAG 1 cut(s) 578
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 1 cut(s) 307
XspI CTAG 5 cut(s) 38, 119, 138, 266, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.