MD01G1167600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015947
Physical Location & Seq
Forward (+)
27221387 .. 27222127
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1167600.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCGTCGCTGGGAGTCCAGTTCGGAGGGAATAACAACGCCGTTCTTGATCACCGCCGTCAGAACAGTCTTTGCATCTGCAGTCCTTCTTCCAAACAGAGTGTTTCGATGATACGTGGATCCGGGGGCAAAAGAGGAAGTGAGGTACTGGCGATGGCGGGGCCGGGATTGGAGGCGGTGGGGCGGGAACGGCGACGAGGCGGTGGGAGCGACGCGGCAGCGGCGGCGTATGAGAAGTTGGACGAGTGGATGAGGGAGTCGGTTGTGGAGATTGTCAAGAATCTCCGGGAAGCACCGCTGTTGGTTAACGTGTACGGGAGAAGCACGGACGGGAGGCCGAGGCTGGAGACGGAGAAGCGGGTGGAGGAGGAGAATTGGGACGGGCTCAGACGGAAATGGGAGGCGGGAGTGGCGCCGTTCCCCGACGGCGTTATCTTTGTGGAAGAATTAGATGATGATAACGGTGACGGAGAGGGTAGCGGTAACGACGAGAGAGGGGATGCGATCACGAGGGCGTGGGGGTTAGTGGTGCAGGGGAGGGGGGAGGAGAGTGCGCCTGCCTGTTACTTGTTGAAGACGAGTAAAGTGGATAGCGGGCCGGGTTTCGGGCTGGGAATGGGTTTGGGTTGCACTCATTTCTGTTTGGTTCGGGTCAATAGTTTCAGGGAGAAGGCCCAGTCGCAGCTCAAGAATTGTTGGCTATTGCAGTCCCAGACCCAGGCCCAGGCCCAGGCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.61

Weight (kDa)

5.63

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7804 PF25089 76 - 155 1.7e-31 Domain of unknown function (DUF7804)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56360 AT5G05250
fragaria_vesca FvH4_7g25910
malus_domestica MD01G1167600.v1.1 MD07G1233600.v1.1
prunus_persica Prupe.2G264000_v2.0.a1
pyrus_communis pycom01g18060 pycom07g21140
rosa_chinensis RchiOBHm_Chr1g0372231
rosa_rugosa Rorug01G0370600
rosa_samantha Rh1AG378900 Rh1BG342400 Rh1CG356000 Rh1DG373600
rosa_wichuraiana Rw1G033340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AccII CGCG 1 cut(s) 215
AclWI GGATC 2 cut(s) 114, 127
AcyI GRCGYC 2 cut(s) 5, 413
AfaI GTAC 2 cut(s) 147, 314
AfiI CCNNNNNNNGG 4 cut(s) 605, 718, 724, 730
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 1 cut(s) 574
AjnI CCWGG 3 cut(s) 717, 723, 729
AluBI AGCT 1 cut(s) 685
AluI AGCT 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 2 cut(s) 114, 127
AoxI GGCC 7 cut(s) 161, 335, 596, 672, 720, 726, 732
ApeKI GCWGC 2 cut(s) 218, 682
AspLEI GCGC 2 cut(s) 415, 556
AspS9I GGNCC 6 cut(s) 161, 596, 673, 721, 727, 733
AsuC2I CCSGG 4 cut(s) 124, 165, 287, 600
AsuHPI GGTGA 2 cut(s) 44, 476
BamHI GGATCC 1 cut(s) 119
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 230, 694
BccI CCATC 1 cut(s) 148
BceAI ACGGC 5 cut(s) 26, 42, 206, 400, 442
BciT130I CCWGG 3 cut(s) 719, 725, 731
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 4 cut(s) 124, 165, 287, 600
BcoDI GTCTC 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 79
BfoI RGCGCY 1 cut(s) 416
BisI GCNGC 5 cut(s) 216, 219, 222, 225, 683
BlsI GCNGC 5 cut(s) 217, 220, 223, 226, 684
Bme1390I CCNGG 7 cut(s) 124, 165, 287, 600, 719, 725, 731
BmgT120I GGNCC 6 cut(s) 161, 596, 673, 721, 727, 733
BmiI GGNNCC 3 cut(s) 121, 162, 414
BmrFI CCNGG 7 cut(s) 124, 165, 287, 600, 719, 725, 731
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 2 cut(s) 84, 490
BmuI ACTGGG 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 581
BpmI CTGGAG 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 671
BpuMI CCSGG 4 cut(s) 124, 165, 287, 600
BsaAI YACGTR 1 cut(s) 116
BsaHI GRCGYC 2 cut(s) 5, 413
BsaJI CCNNGG 5 cut(s) 123, 338, 717, 723, 729
Bsc4I CCNNNNNNNGG 4 cut(s) 605, 718, 724, 730
Bse1I ACTGG 3 cut(s) 19, 153, 676
BseBI CCWGG 3 cut(s) 719, 725, 731
BseDI CCNNGG 5 cut(s) 123, 338, 717, 723, 729
BseGI GGATG 2 cut(s) 255, 505
BseLI CCNNNNNNNGG 4 cut(s) 605, 718, 724, 730
BseMII CTCAG 1 cut(s) 400
BseNI ACTGG 3 cut(s) 19, 153, 676
BseRI GAGGAG 3 cut(s) 380, 383, 560
BseXI GCAGC 2 cut(s) 230, 694
BseYI CCCAGC 2 cut(s) 10, 610
BsgI GTGCAG 1 cut(s) 551
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 7 cut(s) 163, 337, 598, 674, 722, 728, 734
BshNI GGYRCC 1 cut(s) 412
BsiSI CCGG 4 cut(s) 123, 164, 286, 599
BslFI GGGAC 2 cut(s) 392, 694
BslI CCNNNNNNNGG 4 cut(s) 605, 718, 724, 730
BsmAI GTCTC 1 cut(s) 341
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 392, 694
BsnI GGCC 7 cut(s) 163, 337, 598, 674, 722, 728, 734
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 3 cut(s) 49, 119, 504
BspANI GGCC 7 cut(s) 163, 337, 598, 674, 722, 728, 734
BspCNI CTCAG 1 cut(s) 399
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 3 cut(s) 121, 162, 414
BspMAI CTGCAG 1 cut(s) 83
BspPI GGATC 2 cut(s) 114, 127
BspT107I GGYRCC 1 cut(s) 412
BsrI ACTGG 3 cut(s) 19, 153, 676
BssECI CCNNGG 5 cut(s) 123, 338, 717, 723, 729
BssMI GATC 3 cut(s) 49, 119, 504
BssNI GRCGYC 2 cut(s) 5, 413
BssSI CACGAG 1 cut(s) 508
Bst2BI CACGAG 1 cut(s) 508
Bst2UI CCWGG 3 cut(s) 719, 725, 731
Bst4CI ACNGT 2 cut(s) 68, 464
BstACI GRCGYC 2 cut(s) 5, 413
BstBAI YACGTR 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 558, 596
BstDEI CTNAG 1 cut(s) 386
BstF5I GGATG 2 cut(s) 255, 505
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 2 cut(s) 415, 556
BstKTI GATC 3 cut(s) 52, 122, 507
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 3 cut(s) 49, 119, 504
BstMWI GCNNNNNNNGC 5 cut(s) 190, 207, 221, 224, 410
BstNI CCWGG 3 cut(s) 719, 725, 731
BstSCI CCNGG 7 cut(s) 122, 163, 285, 598, 717, 723, 729
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 230, 694
BstV2I GAAGAC 1 cut(s) 581
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BsuRI GGCC 7 cut(s) 163, 337, 598, 674, 722, 728, 734
BtgZI GCGATG 1 cut(s) 167
BtsCI GGATG 2 cut(s) 255, 505
Cac8I GCNNGC 2 cut(s) 558, 596
CfoI GCGC 2 cut(s) 415, 556
Cfr13I GGNCC 6 cut(s) 161, 596, 673, 721, 727, 733
CseI GACGC 1 cut(s) 221
Csp6I GTAC 2 cut(s) 146, 313
CviQI GTAC 2 cut(s) 146, 313
DdeI CTNAG 1 cut(s) 386
DinI GGCGCC 1 cut(s) 414
DpnI GATC 3 cut(s) 51, 121, 506
DpnII GATC 3 cut(s) 49, 119, 504
Eco24I GRGCYC 1 cut(s) 387
EcoRII CCWGG 3 cut(s) 717, 723, 729
EcoT38I GRGCYC 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 341
FaiI YATR 1 cut(s) 231
FaqI GGGAC 2 cut(s) 392, 694
FauI CCCGC 5 cut(s) 151, 177, 351, 397, 587
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 216, 219, 222, 225, 683
FokI GGATG 2 cut(s) 262, 512
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 5 cut(s) 216, 219, 222, 225, 683
GlaI GCGC 2 cut(s) 414, 555
GluI GCNGC 5 cut(s) 216, 219, 222, 225, 683
GsaI CCCAGC 2 cut(s) 14, 614
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 7 cut(s) 163, 337, 598, 674, 722, 728, 734
HapII CCGG 4 cut(s) 123, 164, 286, 599
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 415, 556
Hin1I GRCGYC 2 cut(s) 5, 413
Hin6I GCGC 2 cut(s) 413, 554
HinP1I GCGC 2 cut(s) 413, 554
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 3 cut(s) 15, 257, 280
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 4 cut(s) 123, 164, 286, 599
HphI GGTGA 2 cut(s) 44, 476
Hpy166II GTNNAC 2 cut(s) 307, 313
Hpy188I TCNGA 3 cut(s) 26, 63, 389
Hpy188III TCNNGA 4 cut(s) 47, 277, 508, 688
Hpy8I GTNNAC 2 cut(s) 307, 313
Hpy99I CGWCG 5 cut(s) 10, 198, 215, 428, 491
HpyAV CCTTC 2 cut(s) 96, 664
HpyCH4III ACNGT 2 cut(s) 68, 464
HpyCH4IV ACGT 2 cut(s) 115, 309
HpyCH4V TGCA 5 cut(s) 75, 81, 532, 630, 706
HpyF10VI GCNNNNNNNGC 5 cut(s) 190, 207, 221, 224, 410
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 2 cut(s) 115, 309
Hsp92I GRCGYC 2 cut(s) 5, 413
HspAI GCGC 2 cut(s) 413, 554
KasI GGCGCC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 49
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 3 cut(s) 49, 119, 504
LmnI GCTCC 1 cut(s) 207
Lsp1109I GCAGC 2 cut(s) 230, 694
LweI GCATC 2 cut(s) 84, 490
MaeII ACGT 2 cut(s) 115, 309
MaeIII GTNAC 3 cut(s) 464, 482, 563
MalI GATC 3 cut(s) 51, 121, 506
MboI GATC 3 cut(s) 49, 119, 504
MboII GAAGA 3 cut(s) 81, 455, 586
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 3 cut(s) 373, 446, 691
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 2 cut(s) 24, 266
MmeI TCCRAC 1 cut(s) 219
MseI TTAA 1 cut(s) 306
MspA1I CMGCKG 2 cut(s) 221, 298
MspI CCGG 4 cut(s) 123, 164, 286, 599
MspR9I CCNGG 7 cut(s) 124, 165, 287, 600, 719, 725, 731
MvaI CCWGG 3 cut(s) 719, 725, 731
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 5 cut(s) 190, 207, 221, 224, 410
NarI GGCGCC 1 cut(s) 413
NciI CCSGG 4 cut(s) 124, 165, 287, 600
NdeII GATC 3 cut(s) 49, 119, 504
NlaIV GGNNCC 3 cut(s) 121, 162, 414
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 280
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 5 cut(s) 217, 220, 223, 226, 684
PleI GAGTC 2 cut(s) 23, 265
PluTI GGCGCC 1 cut(s) 416
PpsI GAGTC 2 cut(s) 23, 265
Ppu21I YACGTR 1 cut(s) 116
Psp6I CCWGG 3 cut(s) 717, 723, 729
PspFI CCCAGC 2 cut(s) 10, 610
PspGI CCWGG 3 cut(s) 717, 723, 729
PspN4I GGNNCC 3 cut(s) 121, 162, 414
PspPI GGNCC 6 cut(s) 161, 596, 673, 721, 727, 733
PstI CTGCAG 1 cut(s) 83
PsuI RGATCY 1 cut(s) 119
RsaI GTAC 2 cut(s) 147, 314
RsaNI GTAC 2 cut(s) 146, 313
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 5 cut(s) 216, 219, 222, 225, 683
Sau3AI GATC 3 cut(s) 49, 119, 504
Sau96I GGNCC 6 cut(s) 161, 596, 673, 721, 727, 733
SchI GAGTC 2 cut(s) 24, 266
ScrFI CCNGG 7 cut(s) 124, 165, 287, 600, 719, 725, 731
SduI GDGCHC 1 cut(s) 387
SetI ASST 4 cut(s) 118, 147, 312, 687
SfaNI GCATC 2 cut(s) 84, 490
SfcI CTRYAG 1 cut(s) 79
SfoI GGCGCC 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 3 cut(s) 373, 446, 691
SspDI GGCGCC 1 cut(s) 412
StyD4I CCNGG 7 cut(s) 122, 163, 285, 598, 717, 723, 729
TaaI ACNGT 2 cut(s) 68, 464
TaiI ACGT 2 cut(s) 118, 312
TaqI TCGA 1 cut(s) 107
TasI AATT 3 cut(s) 373, 446, 691
TauI GCSGC 3 cut(s) 218, 224, 227
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 2 cut(s) 218, 682
Tsp45I GTSAC 1 cut(s) 464
TspGWI ACGGA 4 cut(s) 341, 365, 406, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.