pycom01g18060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015947
Physical Location & Seq
Forward (+)
17200969 .. 17201688
720 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18060.1

Sequence Viewer

Length: 720 bp
ATGGCGTCGCTGGGAGTCCAGTTCGGAGGGAATAACAACGCCGTTCTTGATCACCGCCGTCAGAACAGTCTTTGCATCTGCAGTCCTTCCAAACAGAGTGTTTCGATGATACGTGGATCCGGGGGCAAAAGAGGAAGTGAGGTACTGGCGATGGCGGGGGCGGGATTGGAGGCGGTGGGGCGGGAAAGGCGACAAGGCGGTGGGAGCGACGCGGCAGCGGCGGCGTATGAGAAGTTGGACGAGTGGATGAGGGAGTCGGTTGTGGAGATTGTCAAGAATCTCCGGGAAGCACCGCTGTTGGTTAACGTGTACGGGAGAAGCACGGATGGGAGGCCGAGGCTGGAGACGGAGAAGCGGGTGGAGGAGGAGAATTGGGACGGGCTCAGACGGAAATGGGAGACGGGAGTGGCGCCGTTCCCGGACGGCGTTATCTTTGTGGAAGAATTAGATGATGATAACGGTGACGGAGAGGGCATCGGTAACAATGAGGGAGGGGATGCGATCACGAGGGCGTGGGGGTTAGTGGTGCAGGGGAGGGGGGAGGAGAGTGCGCCCGCCTGTTACTTGTTGAAGACGAGTAAAGTGGATAGCGGGCCGGGTTTCGGGCTGGGAATGGGTTTGGGTTGCACTCATTTCTGTTTGGTTCGGGTCAGCAGTTTCAGGGAGAAGGCCCAGTCGCAGCTCAAGAATTGTTGGTTATTGCAGGCCCAGGCCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

25.76

Weight (kDa)

5.43

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7804 PF25089 75 - 154 1.3e-31 Domain of unknown function (DUF7804)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56360 AT5G05250
fragaria_vesca FvH4_7g25910
malus_domestica MD01G1167600.v1.1 MD07G1233600.v1.1
prunus_persica Prupe.2G264000_v2.0.a1
pyrus_communis pycom01g18060 pycom07g21140
rosa_chinensis RchiOBHm_Chr1g0372231
rosa_rugosa Rorug01G0370600
rosa_samantha Rh1AG378900 Rh1BG342400 Rh1CG356000 Rh1DG373600
rosa_wichuraiana Rw1G033340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 409
AccII CGCG 1 cut(s) 212
AclWI GGATC 2 cut(s) 111, 124
AcyI GRCGYC 2 cut(s) 5, 410
AfaI GTAC 2 cut(s) 144, 311
AfiI CCNNNNNNNGG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 1 cut(s) 571
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 1 cut(s) 682
AluI AGCT 1 cut(s) 682
Alw26I GTCTC 2 cut(s) 338, 392
AlwI GGATC 2 cut(s) 111, 124
AoxI GGCC 5 cut(s) 332, 593, 669, 705, 711
ApeKI GCWGC 2 cut(s) 215, 679
AspLEI GCGC 2 cut(s) 412, 553
AspS9I GGNCC 4 cut(s) 593, 670, 706, 712
AsuC2I CCSGG 4 cut(s) 121, 284, 419, 597
AsuHPI GGTGA 2 cut(s) 44, 473
BamHI GGATCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 384
BauI CACGAG 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 578
BbvI GCAGC 2 cut(s) 227, 691
BccI CCATC 2 cut(s) 145, 320
BceAI ACGGC 4 cut(s) 26, 42, 397, 439
BciT130I CCWGG 1 cut(s) 710
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 4 cut(s) 121, 284, 419, 597
BcoDI GTCTC 2 cut(s) 338, 392
BfmI CTRYAG 1 cut(s) 79
BfoI RGCGCY 1 cut(s) 413
BisI GCNGC 5 cut(s) 213, 216, 219, 222, 680
BlsI GCNGC 5 cut(s) 214, 217, 220, 223, 681
Bme1390I CCNGG 5 cut(s) 121, 284, 419, 597, 710
BmgT120I GGNCC 4 cut(s) 593, 670, 706, 712
BmiI GGNNCC 2 cut(s) 118, 411
BmrFI CCNGG 5 cut(s) 121, 284, 419, 597, 710
BmrI ACTGGG 2 cut(s) 667, 709
BmsI GCATC 3 cut(s) 84, 483, 487
BmuI ACTGGG 2 cut(s) 667, 709
BpiI GAAGAC 1 cut(s) 578
BpmI CTGGAG 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 4 cut(s) 121, 284, 419, 597
BsaAI YACGTR 1 cut(s) 113
BsaHI GRCGYC 2 cut(s) 5, 410
BsaJI CCNNGG 3 cut(s) 120, 335, 708
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 19, 150, 673, 715
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 3 cut(s) 120, 335, 708
BseGI GGATG 3 cut(s) 252, 331, 502
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMII CTCAG 1 cut(s) 397
BseNI ACTGG 4 cut(s) 19, 150, 673, 715
BseRI GAGGAG 3 cut(s) 377, 380, 557
BseXI GCAGC 2 cut(s) 227, 691
BseYI CCCAGC 2 cut(s) 10, 607
BsgI GTGCAG 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 5 cut(s) 334, 595, 671, 707, 713
BshNI GGYRCC 1 cut(s) 409
BsiSI CCGG 4 cut(s) 120, 283, 419, 596
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 2 cut(s) 338, 392
BsmBI CGTCTC 2 cut(s) 338, 392
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 5 cut(s) 334, 595, 671, 707, 713
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 3 cut(s) 49, 116, 501
BspANI GGCC 5 cut(s) 334, 595, 671, 707, 713
BspCNI CTCAG 1 cut(s) 396
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 118, 411
BspMAI CTGCAG 1 cut(s) 83
BspPI GGATC 2 cut(s) 111, 124
BspT107I GGYRCC 1 cut(s) 409
BsrI ACTGG 4 cut(s) 19, 150, 673, 715
BssECI CCNNGG 3 cut(s) 120, 335, 708
BssMI GATC 3 cut(s) 49, 116, 501
BssNI GRCGYC 2 cut(s) 5, 410
BssSI CACGAG 1 cut(s) 505
Bst2BI CACGAG 1 cut(s) 505
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 2 cut(s) 68, 461
BstACI GRCGYC 2 cut(s) 5, 410
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 555, 593, 705
BstDEI CTNAG 1 cut(s) 383
BstF5I GGATG 3 cut(s) 252, 331, 502
BstFNI CGCG 1 cut(s) 212
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 2 cut(s) 412, 553
BstKTI GATC 3 cut(s) 52, 119, 504
BstMAI GTCTC 2 cut(s) 338, 392
BstMBI GATC 3 cut(s) 49, 116, 501
BstMWI GCNNNNNNNGC 4 cut(s) 187, 204, 218, 221
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 5 cut(s) 119, 282, 417, 595, 708
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 227, 691
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 5 cut(s) 334, 595, 671, 707, 713
BtgZI GCGATG 1 cut(s) 164
BtsCI GGATG 3 cut(s) 252, 331, 502
Cac8I GCNNGC 3 cut(s) 555, 593, 705
CfoI GCGC 2 cut(s) 412, 553
Cfr13I GGNCC 4 cut(s) 593, 670, 706, 712
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 143, 310
CviJI RGCY 9 cut(s) 334, 340, 382, 595, 607, 671, 682, 707, 713
CviKI_1 RGCY 9 cut(s) 334, 340, 382, 595, 607, 671, 682, 707, 713
CviQI GTAC 2 cut(s) 143, 310
DdeI CTNAG 1 cut(s) 383
DinI GGCGCC 1 cut(s) 411
DpnI GATC 3 cut(s) 51, 118, 503
DpnII GATC 3 cut(s) 49, 116, 501
Eco24I GRGCYC 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 708
EcoT38I GRGCYC 1 cut(s) 384
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
Esp3I CGTCTC 2 cut(s) 338, 392
FaiI YATR 1 cut(s) 228
FaqI GGGAC 1 cut(s) 389
FauI CCCGC 6 cut(s) 148, 154, 174, 348, 562, 584
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 213, 216, 219, 222, 680
FokI GGATG 3 cut(s) 259, 338, 509
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 5 cut(s) 213, 216, 219, 222, 680
GlaI GCGC 2 cut(s) 411, 552
GluI GCNGC 5 cut(s) 213, 216, 219, 222, 680
GsaI CCCAGC 2 cut(s) 14, 611
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 5 cut(s) 334, 595, 671, 707, 713
HapII CCGG 4 cut(s) 120, 283, 419, 596
HgaI GACGC 1 cut(s) 218
HhaI GCGC 2 cut(s) 412, 553
Hin1I GRCGYC 2 cut(s) 5, 410
Hin6I GCGC 2 cut(s) 410, 551
HinP1I GCGC 2 cut(s) 410, 551
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 3 cut(s) 15, 254, 277
HpaI GTTAAC 1 cut(s) 304
HpaII CCGG 4 cut(s) 120, 283, 419, 596
HphI GGTGA 2 cut(s) 44, 473
Hpy166II GTNNAC 2 cut(s) 304, 310
Hpy188I TCNGA 3 cut(s) 26, 63, 386
Hpy188III TCNNGA 4 cut(s) 47, 274, 505, 685
Hpy8I GTNNAC 2 cut(s) 304, 310
Hpy99I CGWCG 2 cut(s) 10, 212
HpyAV CCTTC 2 cut(s) 96, 661
HpyCH4III ACNGT 2 cut(s) 68, 461
HpyCH4IV ACGT 2 cut(s) 112, 306
HpyCH4V TGCA 5 cut(s) 75, 81, 529, 627, 703
HpyF10VI GCNNNNNNNGC 4 cut(s) 187, 204, 218, 221
HpyF3I CTNAG 1 cut(s) 383
HpySE526I ACGT 2 cut(s) 112, 306
Hsp92I GRCGYC 2 cut(s) 5, 410
HspAI GCGC 2 cut(s) 410, 551
KasI GGCGCC 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 49
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 3 cut(s) 49, 116, 501
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 2 cut(s) 227, 691
LweI GCATC 3 cut(s) 84, 483, 487
MaeII ACGT 2 cut(s) 112, 306
MaeIII GTNAC 3 cut(s) 461, 479, 560
MalI GATC 3 cut(s) 51, 118, 503
MboI GATC 3 cut(s) 49, 116, 501
MboII GAAGA 2 cut(s) 452, 583
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 3 cut(s) 370, 443, 688
Mly113I GGCGCC 1 cut(s) 410
MlyI GAGTC 2 cut(s) 24, 263
MmeI TCCRAC 1 cut(s) 216
MseI TTAA 1 cut(s) 303
MspA1I CMGCKG 2 cut(s) 218, 295
MspI CCGG 4 cut(s) 120, 283, 419, 596
MspR9I CCNGG 5 cut(s) 121, 284, 419, 597, 710
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 4 cut(s) 187, 204, 218, 221
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 4 cut(s) 121, 284, 419, 597
NdeII GATC 3 cut(s) 49, 116, 501
NlaIV GGNNCC 2 cut(s) 118, 411
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 277
PfoI TCCNGGA 2 cut(s) 282, 417
PkrI GCNGC 5 cut(s) 214, 217, 220, 223, 681
PleI GAGTC 2 cut(s) 23, 262
PluTI GGCGCC 1 cut(s) 413
PpsI GAGTC 2 cut(s) 23, 262
Ppu21I YACGTR 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 708
PspFI CCCAGC 2 cut(s) 10, 607
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 2 cut(s) 118, 411
PspPI GGNCC 4 cut(s) 593, 670, 706, 712
PstI CTGCAG 1 cut(s) 83
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 2 cut(s) 144, 311
RsaNI GTAC 2 cut(s) 143, 310
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 5 cut(s) 213, 216, 219, 222, 680
Sau3AI GATC 3 cut(s) 49, 116, 501
Sau96I GGNCC 4 cut(s) 593, 670, 706, 712
SchI GAGTC 2 cut(s) 24, 263
ScrFI CCNGG 5 cut(s) 121, 284, 419, 597, 710
SduI GDGCHC 1 cut(s) 384
SetI ASST 4 cut(s) 115, 144, 309, 684
SfaNI GCATC 3 cut(s) 84, 483, 487
SfcI CTRYAG 1 cut(s) 79
SfoI GGCGCC 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 3 cut(s) 370, 443, 688
SspDI GGCGCC 1 cut(s) 409
StyD4I CCNGG 5 cut(s) 119, 282, 417, 595, 708
TaaI ACNGT 2 cut(s) 68, 461
TaiI ACGT 2 cut(s) 115, 309
TaqI TCGA 1 cut(s) 104
TasI AATT 3 cut(s) 370, 443, 688
TauI GCSGC 3 cut(s) 215, 221, 224
TfiI GAWTC 1 cut(s) 277
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 2 cut(s) 215, 679
Tsp45I GTSAC 1 cut(s) 461
TspGWI ACGGA 4 cut(s) 338, 362, 403, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.