pycom07g21140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015947
Physical Location & Seq
Forward (+)
22976611 .. 22977336
726 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21140.1

Sequence Viewer

Length: 726 bp
ATGGCATCGGTTGGAATCCAATTCGGAGGAAATAACAGCGCCGTTCTTGATCACCGCCGTCAGAACGGTCTTTGCGTTTGCAGCCCTTCTTCCAATCGGAGCGTGTCGATGATCCGTGGATCCCGGGGGAAAAGAGGAAATTCGGTAGTGGCCATGGCGCGGGTGGGATTGGAGGCGGTGGGGCGGGAGCGAAGGGGCGGCGGTGGAAAAGGGAGCGAGGCTGCGGCGACTGCGTATGAGAAGTTGGACGCGTGGATGAGGGAGTCGGTGGTGGAGATAGTCCAGAATCTCCGGGAGGCGCCGCTGTTGGTCAATGTGTACGGGAGGAGTAGTACGGACGGTAGGCCGAGGCTGGAGACGGAGAAGCGGGTGGAGGAGGAGAATTGGGACGGGCTCAGACGTAAATGGGAGGCGGGAGTGGCGCCATTTCCCGACGGCGTTATCTTCGTCGAAGAACTGGACGACAATGATAACAGTGGCGGTGAGGGTAACGGTAAAGATGACGGAGGGAATGCGATCACGAAGGCGTGGGGGTTAGTGGTGCAGGGGAAGGGGAAGGAGAGTGGGCCCGCCTGTTACTTGTTGAAGACGAGTAAAGTGAATAACGGGTCGGGCTTCGGGCTCGGTATGGGCTTGGGCTGCACCCATTTCTGTTTGGTTCCGGTGAGCAGTTTCAGGGAGAAGGCCCAATCGCAGCTGAAGAATTGTTGGTTGTTGCAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

25.79

Weight (kDa)

8.75

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7804 PF25089 78 - 158 1.6e-30 Domain of unknown function (DUF7804)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56360 AT5G05250
fragaria_vesca FvH4_7g25910
malus_domestica MD01G1167600.v1.1 MD07G1233600.v1.1
prunus_persica Prupe.2G264000_v2.0.a1
pyrus_communis pycom01g18060 pycom07g21140
rosa_chinensis RchiOBHm_Chr1g0372231
rosa_rugosa Rorug01G0370600
rosa_samantha Rh1AG378900 Rh1BG342400 Rh1CG356000 Rh1DG373600
rosa_wichuraiana Rw1G033340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 298, 421
AccII CGCG 2 cut(s) 160, 251
AclWI GGATC 3 cut(s) 106, 114, 127
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 719
AcyI GRCGYC 2 cut(s) 299, 422
AfaI GTAC 2 cut(s) 320, 334
AfiI CCNNNNNNNGG 1 cut(s) 159
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 1 cut(s) 586
AluBI AGCT 1 cut(s) 697
AluI AGCT 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 3 cut(s) 106, 114, 127
Ama87I CYCGRG 1 cut(s) 123
AoxI GGCC 5 cut(s) 150, 344, 566, 684, 720
ApaI GGGCCC 1 cut(s) 570
ApeKI GCWGC 4 cut(s) 81, 221, 639, 694
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 4 cut(s) 41, 160, 301, 424
AspS9I GGNCC 3 cut(s) 566, 567, 685
AsuC2I CCSGG 3 cut(s) 124, 125, 293
AsuHPI GGTGA 3 cut(s) 44, 494, 676
AvaI CYCGRG 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 570
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 1 cut(s) 119
BanI GGYRCC 2 cut(s) 298, 421
BanII GRGCYC 3 cut(s) 396, 570, 624
BbsI GAAGAC 1 cut(s) 593
BbvI GCAGC 4 cut(s) 93, 208, 626, 706
BceAI ACGGC 3 cut(s) 26, 42, 451
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 3 cut(s) 124, 125, 293
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 724
BfoI RGCGCY 3 cut(s) 42, 302, 425
BisI GCNGC 7 cut(s) 82, 199, 222, 225, 302, 640, 695
BlsI GCNGC 7 cut(s) 83, 200, 223, 226, 303, 641, 696
Bme1390I CCNGG 3 cut(s) 124, 125, 293
BmeT110I CYCGRG 1 cut(s) 123
BmgT120I GGNCC 3 cut(s) 566, 567, 685
BmiI GGNNCC 5 cut(s) 121, 300, 423, 568, 660
BmrFI CCNGG 3 cut(s) 124, 125, 293
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 593
BpmI CTGGAG 1 cut(s) 374
BpuMI CCSGG 3 cut(s) 124, 125, 293
BsaHI GRCGYC 2 cut(s) 299, 422
BsaJI CCNNGG 5 cut(s) 115, 123, 124, 153, 347
BsaWI WCCGGW 1 cut(s) 661
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse1I ACTGG 1 cut(s) 462
BseDI CCNNGG 5 cut(s) 115, 123, 124, 153, 347
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMII CTCAG 1 cut(s) 409
BseNI ACTGG 1 cut(s) 462
BseRI GAGGAG 3 cut(s) 340, 389, 392
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 4 cut(s) 93, 208, 626, 706
BsgI GTGCAG 2 cut(s) 563, 625
Bsh1236I CGCG 2 cut(s) 160, 251
BshFI GGCC 5 cut(s) 152, 346, 568, 686, 722
BshNI GGYRCC 2 cut(s) 298, 421
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 3 cut(s) 124, 292, 662
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 1 cut(s) 159
BsmAI GTCTC 1 cut(s) 350
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 401
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 5 cut(s) 152, 346, 568, 686, 722
BsoBI CYCGRG 1 cut(s) 123
Bsp120I GGGCCC 1 cut(s) 566
Bsp1286I GDGCHC 3 cut(s) 396, 570, 624
Bsp143I GATC 4 cut(s) 49, 111, 119, 516
Bsp19I CCATGG 1 cut(s) 153
BspANI GGCC 5 cut(s) 152, 346, 568, 686, 722
BspCNI CTCAG 1 cut(s) 408
BspFNI CGCG 2 cut(s) 160, 251
BspLI GGNNCC 5 cut(s) 121, 300, 423, 568, 660
BspPI GGATC 3 cut(s) 106, 114, 127
BspT107I GGYRCC 2 cut(s) 298, 421
BsrI ACTGG 1 cut(s) 462
BssECI CCNNGG 5 cut(s) 115, 123, 124, 153, 347
BssMI GATC 4 cut(s) 49, 111, 119, 516
BssNI GRCGYC 2 cut(s) 299, 422
BssT1I CCWWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 68, 341, 476, 494
BstACI GRCGYC 2 cut(s) 299, 422
BstC8I GCNNGC 2 cut(s) 570, 720
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 2 cut(s) 115, 153
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 2 cut(s) 160, 251
BstH2I RGCGCY 3 cut(s) 42, 302, 425
BstHHI GCGC 4 cut(s) 41, 160, 301, 424
BstKTI GATC 4 cut(s) 52, 114, 122, 519
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 4 cut(s) 49, 111, 119, 516
BstMWI GCNNNNNNNGC 4 cut(s) 81, 230, 419, 639
BstSCI CCNGG 3 cut(s) 122, 123, 291
BstSLI GKGCMC 1 cut(s) 570
BstUI CGCG 2 cut(s) 160, 251
BstV1I GCAGC 4 cut(s) 93, 208, 626, 706
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BsuRI GGCC 5 cut(s) 152, 346, 568, 686, 722
BtgI CCRYGG 2 cut(s) 115, 153
BtsCI GGATG 1 cut(s) 261
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 570, 720
CfoI GCGC 4 cut(s) 41, 160, 301, 424
Cfr13I GGNCC 3 cut(s) 566, 567, 685
Cfr9I CCCGGG 1 cut(s) 123
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 319, 333
CviAII CATG 1 cut(s) 154
CviQI GTAC 2 cut(s) 319, 333
DdeI CTNAG 1 cut(s) 395
DinI GGCGCC 2 cut(s) 300, 423
DpnI GATC 4 cut(s) 51, 113, 121, 518
DpnII GATC 4 cut(s) 49, 111, 119, 516
EaeI YGGCCR 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 722
Eco24I GRGCYC 3 cut(s) 396, 570, 624
Eco57I CTGAAG 1 cut(s) 719
Eco88I CYCGRG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 3 cut(s) 396, 570, 624
EgeI GGCGCC 2 cut(s) 300, 423
EheI GGCGCC 2 cut(s) 300, 423
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 1 cut(s) 157
FaiI YATR 3 cut(s) 155, 237, 629
FaqI GGGAC 1 cut(s) 401
FatI CATG 1 cut(s) 153
FauI CCCGC 5 cut(s) 153, 177, 360, 406, 577
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 7 cut(s) 82, 199, 222, 225, 302, 640, 695
FokI GGATG 1 cut(s) 268
FriOI GRGCYC 3 cut(s) 396, 570, 624
Fsp4HI GCNGC 7 cut(s) 82, 199, 222, 225, 302, 640, 695
FspBI CTAG 1 cut(s) 724
GlaI GCGC 4 cut(s) 40, 159, 300, 423
GluI GCNGC 7 cut(s) 82, 199, 222, 225, 302, 640, 695
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 3 cut(s) 42, 302, 425
HaeIII GGCC 5 cut(s) 152, 346, 568, 686, 722
HapII CCGG 3 cut(s) 124, 292, 662
HgaI GACGC 1 cut(s) 257
HhaI GCGC 4 cut(s) 41, 160, 301, 424
Hin1I GRCGYC 2 cut(s) 299, 422
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 4 cut(s) 39, 158, 299, 422
HinP1I GCGC 4 cut(s) 39, 158, 299, 422
HinfI GANTC 3 cut(s) 15, 263, 286
HpaII CCGG 3 cut(s) 124, 292, 662
HphI GGTGA 3 cut(s) 44, 494, 676
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 4 cut(s) 26, 63, 99, 398
Hpy188III TCNNGA 4 cut(s) 47, 283, 431, 520
Hpy8I GTNNAC 1 cut(s) 319
Hpy99I CGWCG 2 cut(s) 437, 452
HpyAV CCTTC 6 cut(s) 96, 186, 517, 544, 550, 676
HpyCH4III ACNGT 4 cut(s) 68, 341, 476, 494
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 4 cut(s) 81, 544, 642, 718
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 230, 419, 639
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 1 cut(s) 400
Hsp92I GRCGYC 2 cut(s) 299, 422
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 4 cut(s) 39, 158, 299, 422
KasI GGCGCC 2 cut(s) 298, 421
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 4 cut(s) 49, 111, 119, 516
LmnI GCTCC 3 cut(s) 99, 187, 213
Lsp1109I GCAGC 4 cut(s) 93, 208, 626, 706
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 724
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 2 cut(s) 488, 575
MalI GATC 4 cut(s) 51, 113, 121, 518
MboI GATC 4 cut(s) 49, 111, 119, 516
MboII GAAGA 5 cut(s) 81, 436, 464, 598, 712
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 3 cut(s) 396, 570, 624
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 4 cut(s) 20, 139, 382, 703
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 152
Mly113I GGCGCC 2 cut(s) 299, 422
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 225
Mox20I TGGCCA 1 cut(s) 152
MscI TGGCCA 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 2 cut(s) 304, 697
MspI CCGG 3 cut(s) 124, 292, 662
MspR9I CCNGG 3 cut(s) 124, 125, 293
Mva1269I GAATGC 1 cut(s) 517
MvnI CGCG 2 cut(s) 160, 251
MwoI GCNNNNNNNGC 4 cut(s) 81, 230, 419, 639
NarI GGCGCC 2 cut(s) 299, 422
NciI CCSGG 3 cut(s) 124, 125, 293
NcoI CCATGG 1 cut(s) 153
NdeII GATC 4 cut(s) 49, 111, 119, 516
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 5 cut(s) 121, 300, 423, 568, 660
NmeAIII GCCGAG 1 cut(s) 372
PceI AGGCCT 1 cut(s) 722
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 2 cut(s) 15, 286
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 7 cut(s) 83, 200, 223, 226, 303, 641, 696
PleI GAGTC 1 cut(s) 271
PluTI GGCGCC 2 cut(s) 302, 425
PpsI GAGTC 1 cut(s) 271
PspN4I GGNNCC 5 cut(s) 121, 300, 423, 568, 660
PspOMI GGGCCC 1 cut(s) 566
PspPI GGNCC 3 cut(s) 566, 567, 685
PsuI RGATCY 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 697
RsaI GTAC 2 cut(s) 320, 334
RsaNI GTAC 2 cut(s) 319, 333
SatI GCNGC 7 cut(s) 82, 199, 222, 225, 302, 640, 695
Sau3AI GATC 4 cut(s) 49, 111, 119, 516
Sau96I GGNCC 3 cut(s) 566, 567, 685
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 3 cut(s) 124, 125, 293
SduI GDGCHC 3 cut(s) 396, 570, 624
SetI ASST 2 cut(s) 403, 699
SfaNI GCATC 1 cut(s) 14
SfoI GGCGCC 2 cut(s) 300, 423
SmaI CCCGGG 1 cut(s) 125
Sse9I AATT 4 cut(s) 20, 139, 382, 703
SseBI AGGCCT 1 cut(s) 722
SspDI GGCGCC 2 cut(s) 298, 421
SspMI CTAG 1 cut(s) 724
StuI AGGCCT 1 cut(s) 722
StyD4I CCNGG 3 cut(s) 122, 123, 291
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 4 cut(s) 68, 341, 476, 494
TaiI ACGT 1 cut(s) 403
TaqI TCGA 2 cut(s) 107, 450
TasI AATT 4 cut(s) 20, 139, 382, 703
TauI GCSGC 3 cut(s) 201, 227, 304
TfiI GAWTC 2 cut(s) 15, 286
TscAI CASTG 1 cut(s) 481
TseI GCWGC 4 cut(s) 81, 221, 639, 694
TspGWI ACGGA 4 cut(s) 104, 350, 374, 519
TspMI CCCGGG 1 cut(s) 123
TspRI CASTG 1 cut(s) 481
XapI RAATTY 1 cut(s) 139
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XmaI CCCGGG 1 cut(s) 123
XspI CTAG 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.