FvH4_7g25910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015947
Physical Location & Seq
Forward (+)
19711111 .. 19712505
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25910.t1

Sequence Viewer

Length: 780 bp
ATGGCCTCGTCGCTGTGCGGAGGGAGCATCCACCATTCTTTGAGCAGCGTTCGTCTCCAACAAGATCGGAATCTTCGCTCCGATCAGCCGTCTTCTTGTTCCATGGTGATTAATATTCCTCGTCGCGTCAGGGGTAGAGTCGTAAATTCCGTCTCTAGGGGTAAAATCGTAAATTCGTCATCCGCGGCTGTGGCGGAGACGCGGAGTCCTCGGCGGAACCGGAGGGGTGGAGACAGGAAAGGGAGCGAGACGGCGGCGGCGACGGCGTACGAGAGGGTAGACGAGTGGATGAAGGATTCGGTGACGGAGATCGTCAAGAACCTCCGGGAGGCGCCGCTGCTGGTCCACGTGTACGGCGGCGACGAGGAGGAACGGAGGATGGAGACGGAGAAGAGAGTGGAGGAGGAGAATTGGGACACGCTGAAGGGGAAGTGGGAGGCTGGGGAGGCGCCGTCACCGGAAGGCGTTATATTCGTGGAGGAGCTGATGGACGACGGCGGAGTTGACGGTGGAGATAACGGAGGGGAATATTCCGTTAGTAAGGATGGGATCACGAGGGCGTGGGGGCTGGTGGTGCAGGGGAAAGGGGAGGAGTGTGGGCCAGCTTGTTATTTGTTGAAGACGAGTAAAGTTAATGTCGGGTCGGGTTATGGGCTGGGGATGGGCATGGGCTGCACTCATTTTTGCTTGGTTCGGGTCAAGAGTTTTAGGGAGACGGCCAAGTCGCAGCTCAAGAACAGTTGGCTGTTGCAGGCACAGATGCAAGGTCTGGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

28.32

Weight (kDa)

8.19

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7804 PF25089 89 - 165 3.3e-31 Domain of unknown function (DUF7804)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56360 AT5G05250
fragaria_vesca FvH4_7g25910
malus_domestica MD01G1167600.v1.1 MD07G1233600.v1.1
prunus_persica Prupe.2G264000_v2.0.a1
pyrus_communis pycom01g18060 pycom07g21140
rosa_chinensis RchiOBHm_Chr1g0372231
rosa_rugosa Rorug01G0370600
rosa_samantha Rh1AG378900 Rh1BG342400 Rh1CG356000 Rh1DG373600
rosa_wichuraiana Rw1G033340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 721
AccB1I GGYRCC 2 cut(s) 331, 448
AccI GTMKAC 1 cut(s) 279
AccII CGCG 3 cut(s) 126, 185, 202
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 2 cut(s) 145, 172
AcuI CTGAAG 1 cut(s) 443
AcvI CACGTG 1 cut(s) 349
AcyI GRCGYC 2 cut(s) 332, 449
AfaI GTAC 2 cut(s) 269, 353
AfiI CCNNNNNNNGG 2 cut(s) 156, 328
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 1 cut(s) 619
AhdI GACNNNNNGTC 1 cut(s) 204
AluBI AGCT 3 cut(s) 484, 605, 730
AluI AGCT 3 cut(s) 484, 605, 730
Alw26I GTCTC 7 cut(s) 59, 157, 191, 225, 242, 377, 707
AlwI GGATC 1 cut(s) 557
AoxI GGCC 3 cut(s) 3, 599, 717
ApeKI GCWGC 4 cut(s) 45, 337, 672, 727
ApoI RAATTY 2 cut(s) 145, 172
AseI ATTAAT 1 cut(s) 111
AspLEI GCGC 2 cut(s) 334, 451
AspS9I GGNCC 2 cut(s) 343, 599
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 3 cut(s) 118, 313, 447
AvaII GGWCC 1 cut(s) 343
BanI GGYRCC 2 cut(s) 331, 448
BauI CACGAG 1 cut(s) 553
BbrPI CACGTG 1 cut(s) 349
BbsI GAAGAC 2 cut(s) 84, 626
BbvI GCAGC 4 cut(s) 57, 324, 659, 739
BccI CCATC 4 cut(s) 373, 481, 539, 655
BceAI ACGGC 7 cut(s) 73, 267, 279, 370, 436, 511, 732
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 7 cut(s) 59, 157, 191, 225, 242, 377, 707
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 2 cut(s) 335, 452
BisI GCNGC 9 cut(s) 46, 186, 255, 258, 335, 338, 358, 673, 728
BlsI GCNGC 9 cut(s) 47, 187, 256, 259, 336, 339, 359, 674, 729
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 343
BmeRI GACNNNNNGTC 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 343, 599
BmiI GGNNCC 3 cut(s) 218, 333, 450
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 2 cut(s) 36, 750
BpiI GAAGAC 2 cut(s) 84, 626
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 2 cut(s) 332, 449
BsaJI CCNNGG 3 cut(s) 102, 183, 209
BsaWI WCCGGW 2 cut(s) 219, 457
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 328
Bse8I GATNNNNATC 1 cut(s) 69
BseDI CCNNGG 3 cut(s) 102, 183, 209
BseGI GGATG 6 cut(s) 27, 179, 294, 384, 550, 666
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 156, 328
BseRI GAGGAG 5 cut(s) 380, 416, 419, 494, 605
BseXI GCAGC 4 cut(s) 57, 324, 659, 739
BseYI CCCAGC 2 cut(s) 440, 655
BsgI GTGCAG 2 cut(s) 596, 658
Bsh1236I CGCG 3 cut(s) 126, 185, 202
BshFI GGCC 3 cut(s) 5, 601, 719
BshNI GGYRCC 2 cut(s) 331, 448
BsiSI CCGG 3 cut(s) 220, 325, 458
BsiWI CGTACG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 156, 328
BsmAI GTCTC 7 cut(s) 59, 157, 191, 225, 242, 377, 707
BsmBI CGTCTC 6 cut(s) 59, 157, 191, 242, 377, 707
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 3 cut(s) 5, 601, 719
Bsp143I GATC 4 cut(s) 64, 82, 309, 549
Bsp19I CCATGG 1 cut(s) 102
BspANI GGCC 3 cut(s) 5, 601, 719
BspFNI CGCG 3 cut(s) 126, 185, 202
BspLI GGNNCC 3 cut(s) 218, 333, 450
BspPI GGATC 1 cut(s) 557
BspT107I GGYRCC 2 cut(s) 331, 448
BssECI CCNNGG 3 cut(s) 102, 183, 209
BssMI GATC 4 cut(s) 64, 82, 309, 549
BssNI GRCGYC 2 cut(s) 332, 449
BssSI CACGAG 1 cut(s) 553
BssT1I CCWWGG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 553
Bst4CI ACNGT 2 cut(s) 509, 740
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 2 cut(s) 332, 449
BstAPI GCANNNNNTGC 1 cut(s) 672
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 603, 753
BstDSI CCRYGG 2 cut(s) 102, 183
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 6 cut(s) 27, 179, 294, 384, 550, 666
BstFNI CGCG 3 cut(s) 126, 185, 202
BstH2I RGCGCY 2 cut(s) 335, 452
BstHHI GCGC 2 cut(s) 334, 451
BstKTI GATC 4 cut(s) 67, 85, 312, 552
BstMAI GTCTC 7 cut(s) 59, 157, 191, 225, 242, 377, 707
BstMBI GATC 4 cut(s) 64, 82, 309, 549
BstMWI GCNNNNNNNGC 6 cut(s) 24, 191, 263, 446, 574, 672
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 3 cut(s) 126, 185, 202
BstV1I GCAGC 4 cut(s) 57, 324, 659, 739
BstV2I GAAGAC 2 cut(s) 84, 626
BsuRI GGCC 3 cut(s) 5, 601, 719
BtgI CCRYGG 2 cut(s) 102, 183
BtsCI GGATG 6 cut(s) 27, 179, 294, 384, 550, 666
Cac8I GCNNGC 2 cut(s) 603, 753
CfoI GCGC 2 cut(s) 334, 451
Cfr13I GGNCC 2 cut(s) 343, 599
Cfr42I CCGCGG 1 cut(s) 186
CseI GACGC 2 cut(s) 115, 208
Csp6I GTAC 2 cut(s) 268, 352
CviAII CATG 2 cut(s) 103, 667
CviQI GTAC 2 cut(s) 268, 352
DinI GGCGCC 2 cut(s) 333, 450
DpnI GATC 4 cut(s) 66, 84, 311, 551
DpnII GATC 4 cut(s) 64, 82, 309, 549
DrdI GACNNNNNNGTC 1 cut(s) 721
DriI GACNNNNNGTC 1 cut(s) 204
DseDI GACNNNNNNGTC 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 386
Eam1105I GACNNNNNGTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 3 cut(s) 209, 229, 513
Eco130I CCWWGG 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 102
EgeI GGCGCC 2 cut(s) 333, 450
EheI GGCGCC 2 cut(s) 333, 450
ErhI CCWWGG 1 cut(s) 102
Esp3I CGTCTC 6 cut(s) 59, 157, 191, 242, 377, 707
FaeI CATG 2 cut(s) 106, 670
FaiI YATR 4 cut(s) 104, 470, 651, 668
FaqI GGGAC 1 cut(s) 428
FatI CATG 2 cut(s) 102, 666
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 9 cut(s) 46, 186, 255, 258, 335, 338, 358, 673, 728
FokI GGATG 6 cut(s) 14, 166, 301, 391, 557, 673
Fsp4HI GCNGC 9 cut(s) 46, 186, 255, 258, 335, 338, 358, 673, 728
FspBI CTAG 1 cut(s) 156
GlaI GCGC 2 cut(s) 333, 450
GluI GCNGC 9 cut(s) 46, 186, 255, 258, 335, 338, 358, 673, 728
GsaI CCCAGC 2 cut(s) 444, 659
HaeII RGCGCY 2 cut(s) 335, 452
HaeIII GGCC 3 cut(s) 5, 601, 719
HapII CCGG 3 cut(s) 220, 325, 458
HgaI GACGC 2 cut(s) 115, 208
HhaI GCGC 2 cut(s) 334, 451
Hin1I GRCGYC 2 cut(s) 332, 449
Hin1II CATG 2 cut(s) 106, 670
Hin6I GCGC 2 cut(s) 332, 449
HinP1I GCGC 2 cut(s) 332, 449
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 4 cut(s) 70, 138, 205, 296
HpaII CCGG 3 cut(s) 220, 325, 458
HphI GGTGA 3 cut(s) 118, 313, 447
Hpy166II GTNNAC 4 cut(s) 280, 346, 352, 505
Hpy188I TCNGA 2 cut(s) 69, 82
Hpy188III TCNNGA 4 cut(s) 316, 553, 700, 733
Hpy8I GTNNAC 4 cut(s) 280, 346, 352, 505
Hpy99I CGWCG 5 cut(s) 13, 126, 265, 365, 497
HpyAV CCTTC 3 cut(s) 286, 418, 455
HpyCH4III ACNGT 2 cut(s) 509, 740
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 4 cut(s) 577, 675, 751, 763
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 191, 263, 446, 574, 672
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 2 cut(s) 332, 449
Hsp92II CATG 2 cut(s) 106, 670
HspAI GCGC 2 cut(s) 332, 449
KasI GGCGCC 2 cut(s) 331, 448
KspI CCGCGG 1 cut(s) 186
Kzo9I GATC 4 cut(s) 64, 82, 309, 549
LmnI GCTCC 4 cut(s) 24, 83, 243, 481
Lsp1109I GCAGC 4 cut(s) 57, 324, 659, 739
LweI GCATC 2 cut(s) 36, 750
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 301, 453
MalI GATC 4 cut(s) 66, 84, 311, 551
MboI GATC 4 cut(s) 64, 82, 309, 549
MboII GAAGA 4 cut(s) 65, 84, 403, 631
MluCI AATT 3 cut(s) 145, 172, 409
Mly113I GGCGCC 2 cut(s) 332, 449
MlyI GAGTC 2 cut(s) 147, 214
MmeI TCCRAC 1 cut(s) 82
MseI TTAA 2 cut(s) 111, 633
MspA1I CMGCKG 2 cut(s) 185, 337
MspI CCGG 3 cut(s) 220, 325, 458
MspR9I CCNGG 1 cut(s) 326
MvnI CGCG 3 cut(s) 126, 185, 202
MwoI GCNNNNNNNGC 6 cut(s) 24, 191, 263, 446, 574, 672
NarI GGCGCC 2 cut(s) 332, 449
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 102
NdeII GATC 4 cut(s) 64, 82, 309, 549
NlaIII CATG 2 cut(s) 106, 670
NlaIV GGNNCC 3 cut(s) 218, 333, 450
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 301, 453
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 257, 360
PfeI GAWTC 2 cut(s) 70, 296
Pfl23II CGTACG 1 cut(s) 267
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 9 cut(s) 47, 187, 256, 259, 336, 339, 359, 674, 729
PleI GAGTC 2 cut(s) 146, 213
PluTI GGCGCC 2 cut(s) 335, 452
PmaCI CACGTG 1 cut(s) 349
PmlI CACGTG 1 cut(s) 349
PpsI GAGTC 2 cut(s) 146, 213
Ppu21I YACGTR 1 cut(s) 349
PshBI ATTAAT 1 cut(s) 111
PspCI CACGTG 1 cut(s) 349
PspFI CCCAGC 2 cut(s) 440, 655
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 3 cut(s) 218, 333, 450
PspPI GGNCC 2 cut(s) 343, 599
RsaI GTAC 2 cut(s) 269, 353
RsaNI GTAC 2 cut(s) 268, 352
SacII CCGCGG 1 cut(s) 186
SaqAI TTAA 2 cut(s) 111, 633
SatI GCNGC 9 cut(s) 46, 186, 255, 258, 335, 338, 358, 673, 728
Sau3AI GATC 4 cut(s) 64, 82, 309, 549
Sau96I GGNCC 2 cut(s) 343, 599
SchI GAGTC 2 cut(s) 147, 214
ScrFI CCNGG 1 cut(s) 326
SetI ASST 6 cut(s) 324, 351, 486, 607, 732, 769
SfaNI GCATC 2 cut(s) 36, 750
SfoI GGCGCC 2 cut(s) 333, 450
Sfr303I CCGCGG 1 cut(s) 186
SgrBI CCGCGG 1 cut(s) 186
SinI GGWCC 1 cut(s) 343
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 3 cut(s) 145, 172, 409
SspDI GGCGCC 2 cut(s) 331, 448
SspI AATATT 2 cut(s) 115, 530
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 2 cut(s) 509, 740
TaiI ACGT 1 cut(s) 351
TasI AATT 3 cut(s) 145, 172, 409
TauI GCSGC 5 cut(s) 188, 257, 260, 337, 360
TfiI GAWTC 2 cut(s) 70, 296
Tru1I TTAA 2 cut(s) 111, 633
Tru9I TTAA 2 cut(s) 111, 633
TseFI GTSAC 2 cut(s) 301, 453
TseI GCWGC 4 cut(s) 45, 337, 672, 727
Tsp45I GTSAC 2 cut(s) 301, 453
TspDTI ATGAA 1 cut(s) 305
TspGWI ACGGA 6 cut(s) 139, 320, 388, 401, 523, 534
VpaK11BI GGWCC 1 cut(s) 343
VspI ATTAAT 1 cut(s) 111
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 2 cut(s) 145, 172
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.