AT5G05740

zinc metalloprotease EGY2 chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008714
Physical Location & Seq
Reverse (-)
1723687 .. 1727068
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05740.1

Sequence Viewer

Length: 1671 bp
ATGAATCTCGCGGTGGCTAGTTTTCGCGGCAATTTCGGCGTCTTGTCGCAATGCAGCTCATGTTGTAGTCTTCAGTTTCAGCCTTTTGTAGCTGCTACTTCCAGTCTCAATTTTGGGCAAACAGGAACATCTCGAAGGAAGAAGGATCTGAAGCTTGAAAGAGTTTTCAGAAAGAGAGAGACTTTGGTTAGAGTGACGGAGACGCAAACGGAACCAGAAGGCAATGATGATGAGGATAATAAAGAAGGCAAAGAGAGCTCTGCAGATGATCCTCCTACAAAAATTCCTACTGAGTTGAATTCACAGTCAACGGTTGTGAATGAAGCGCCGGGCAATGAGGAGGAAAACAAAGCTCAGTTCAGTTCTCAGGACGGTGATAAATTAGAAGTTAGCAGTGGTTCTCCTCTTCCTGGTGTGAATGTAAGTATTATCATCCATGTTATTTACAAAGACGACAGCATAATGTTTTCTGGTTGTTTATCTTTCATAAAAAGTTGTTGTGAACAGCCATTACAATTGGACGATTCCATGAGACTCCCCAAGGAAACCATTGATATTCTCAGGGGCCAAGTATTTGGATTTGACACCTTCTTCGTAACAAGCCAAGAACCATATGAGGGTGGGGTATTGTTCAAAGGAAATCTAAGAGGCAAGCCTGCTACAAGTTACGAGAAGATAAAGACGAGGATGGAGAATAATTTTGGTGACCAGTACAAGCTTTTCCTTCTCACGAATCCTGAGGATGATAAACCTGTGGCCGTTGTTGTTCCAAGAAGGTCCTTAGAACCCGAGACAACAGCTGTTCCTGAATGGTTTGCTGCTGGTTCTTTTGGATTGGTGGCATTGTTTACATTGTTTCTCCGTAATGTGCCTGCCTTGCAATCCGACTTACTATCGGCCTTTGACAACCTTGAACTGTTAAAAGATGGCTTACCAGGAGCTCTTGTCACCGCTCTTGTCCTTGGAGTACATGAACTGGGACACATTTTAGTTGCAAATAGCTTAGGAATAAAGCTTGGTGTTCCATTCTTTGTTCCAAGTTGGCAGATAGGCTCTTTCGGTGCTATAACGAGAATCAAAAATATCGTAGCCAAGCGAGAAGATCTTTTGAAGGTTGCAGCTGCAGGACCTTTGGCTGGCTTTTCTCTAGGCTTGATTCTGTTTCTTATAGGGTTATTCGTGCCTCCCAGTGACGGCATTGGCGTTGTAGTTGATGCATCAGTGTTTCACGAGTCCTTCCTTGCTGGTGGTATTGCAAAGCTCCTACTAGGTGATGCACTCAAAGAAGGAACTTCAATATCTCTTAACCCACTTGTGATCTGGGCATGGGCTGGACTTCTCATCAATGGCATTAACAGTATCCCTGCGGGGGAGCTCGATGGGGGCAAAATAGCTTTCTCCATTTGGGGAAGAAAGACCGCAACACGGCTCACGGGTGCATCCATAGCGCTTTTGGGCTTATCGGCGTTGTTCAGCGACGTGGCATTCTACTGGGTGGTTCTGATATTCTTCCTGCAGCGTGGACCGATTGCGCCGCTGGCTGAAGAAATCACGGTGCCTGATGATAAGTATGTGTCTCTTGGAATCCTGGTTTTGTTTCTCTCCTTGCTTGTGTGTTTGCCTTACCCATTTGCTTTCACGGGCAATGAAGCCATGATGATTGGCTTGTGA

Protein Analysis

556

Amino Acids

60.06

Weight (kDa)

4.99

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50 PF02163 314 - 472 6.3e-14 Peptidase family M50
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1555
AccBSI CCGCTC 1 cut(s) 953
AccII CGCG 2 cut(s) 11, 27
AciI CCGC 6 cut(s) 11, 27, 951, 1367, 1419, 1535
AclWI GGATC 2 cut(s) 153, 263
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 2 cut(s) 282, 298
AcuI CTGAAG 3 cut(s) 56, 170, 1563
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 2 cut(s) 713, 969
AfeI AGCGCT 1 cut(s) 1449
AfiI CCNNNNNNNGG 6 cut(s) 410, 617, 1136, 1193, 1369, 1425
AgsI TTSAA 6 cut(s) 158, 298, 634, 914, 1111, 1296
AjiI CACGTC 1 cut(s) 1480
AjnI CCWGG 3 cut(s) 409, 934, 1587
Alw21I GWGCWC 3 cut(s) 260, 943, 1377
Alw26I GTCTC 6 cut(s) 110, 173, 194, 526, 785, 1581
AlwI GGATC 2 cut(s) 153, 263
Ama87I CYCGRG 1 cut(s) 788
Aor51HI AGCGCT 1 cut(s) 1449
AoxI GGCC 3 cut(s) 565, 756, 897
ApeKI GCWGC 6 cut(s) 54, 92, 818, 1118, 1121, 1516
ApoI RAATTY 2 cut(s) 282, 298
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AspLEI GCGC 3 cut(s) 328, 1450, 1534
AspS9I GGNCC 4 cut(s) 565, 777, 1127, 1523
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 4 cut(s) 386, 716, 940, 1283
AvaI CYCGRG 1 cut(s) 788
AvaII GGWCC 3 cut(s) 777, 1127, 1523
AxyI CCTNAGG 1 cut(s) 738
BanI GGYRCC 1 cut(s) 1555
BanII GRGCYC 3 cut(s) 260, 943, 1377
BauI CACGAG 1 cut(s) 1229
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 3 cut(s) 260, 943, 1377
BbvI GCAGC 6 cut(s) 66, 79, 805, 1108, 1130, 1528
BccI CCATC 3 cut(s) 682, 920, 1373
BceAI ACGGC 3 cut(s) 743, 1210, 1442
BciT130I CCWGG 3 cut(s) 411, 936, 1589
BciVI GTATCC 1 cut(s) 1370
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 6 cut(s) 110, 173, 194, 526, 785, 1581
BfaI CTAG 3 cut(s) 18, 1148, 1268
BfmI CTRYAG 3 cut(s) 261, 1122, 1514
BfoI RGCGCY 2 cut(s) 329, 1451
BfuI GTATCC 1 cut(s) 1370
BglII AGATCT 1 cut(s) 1102
BisI GCNGC 8 cut(s) 28, 55, 93, 819, 1119, 1122, 1517, 1535
BlsI GCNGC 8 cut(s) 29, 56, 94, 820, 1120, 1123, 1518, 1536
Bme1390I CCNGG 4 cut(s) 330, 411, 936, 1589
Bme18I GGWCC 3 cut(s) 777, 1127, 1523
BmeT110I CYCGRG 1 cut(s) 788
BmgBI CACGTC 1 cut(s) 1480
BmgT120I GGNCC 4 cut(s) 565, 777, 1127, 1523
BmiI GGNNCC 3 cut(s) 213, 566, 1557
BmrFI CCNGG 4 cut(s) 330, 411, 936, 1589
BmrI ACTGGG 3 cut(s) 986, 1182, 1501
BmsI GCATC 4 cut(s) 1204, 1226, 1264, 1448
BmuI ACTGGG 3 cut(s) 986, 1182, 1501
BpiI GAAGAC 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 1003
BpuMI CCSGG 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 540, 961
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 6 cut(s) 410, 617, 1136, 1193, 1369, 1425
Bse1I ACTGG 5 cut(s) 102, 709, 981, 1188, 1496
Bse21I CCTNAGG 1 cut(s) 738
Bse3DI GCAATG 4 cut(s) 56, 229, 340, 1651
BseBI CCWGG 3 cut(s) 411, 936, 1589
BseDI CCNNGG 2 cut(s) 540, 961
BseGI GGATG 4 cut(s) 432, 693, 748, 1439
BseLI CCNNNNNNNGG 6 cut(s) 410, 617, 1136, 1193, 1369, 1425
BseMI GCAATG 4 cut(s) 56, 229, 340, 1651
BseMII CTCAG 5 cut(s) 282, 368, 380, 574, 729
BseNI ACTGG 5 cut(s) 102, 709, 981, 1188, 1496
BseRI GAGGAG 2 cut(s) 353, 393
BseXI GCAGC 6 cut(s) 66, 79, 805, 1108, 1130, 1528
Bsh1236I CGCG 2 cut(s) 11, 27
BshFI GGCC 3 cut(s) 567, 758, 899
BshNI GGYRCC 1 cut(s) 1555
BsiHKAI GWGCWC 3 cut(s) 260, 943, 1377
BsiHKCI CYCGRG 1 cut(s) 788
BsiSI CCGG 1 cut(s) 329
BslFI GGGAC 1 cut(s) 993
BslI CCNNNNNNNGG 6 cut(s) 410, 617, 1136, 1193, 1369, 1425
BsmAI GTCTC 6 cut(s) 110, 173, 194, 526, 785, 1581
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 993
BsmI GAATGC 1 cut(s) 1484
BsnI GGCC 3 cut(s) 567, 758, 899
BsoBI CYCGRG 1 cut(s) 788
Bsp1286I GDGCHC 3 cut(s) 260, 943, 1377
Bsp143I GATC 4 cut(s) 145, 268, 1102, 1317
BspACI CCGC 6 cut(s) 11, 27, 951, 1367, 1419, 1535
BspANI GGCC 3 cut(s) 567, 758, 899
BspCNI CTCAG 5 cut(s) 283, 367, 379, 573, 730
BspFNI CGCG 2 cut(s) 11, 27
BspLI GGNNCC 3 cut(s) 213, 566, 1557
BspMAI CTGCAG 3 cut(s) 265, 1126, 1518
BspPI GGATC 2 cut(s) 153, 263
BspT107I GGYRCC 1 cut(s) 1555
BsrBI CCGCTC 1 cut(s) 953
BsrDI GCAATG 4 cut(s) 56, 229, 340, 1651
BsrI ACTGG 5 cut(s) 102, 709, 981, 1188, 1496
BssECI CCNNGG 2 cut(s) 540, 961
BssMI GATC 4 cut(s) 145, 268, 1102, 1317
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 1229
BssT1I CCWWGG 2 cut(s) 540, 961
Bst2BI CACGAG 1 cut(s) 1229
Bst2UI CCWGG 3 cut(s) 411, 936, 1589
Bst4CI ACNGT 6 cut(s) 306, 313, 374, 918, 1358, 1555
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 5 cut(s) 653, 657, 873, 1138, 1539
BstDEI CTNAG 8 cut(s) 291, 354, 366, 560, 644, 738, 781, 1003
BstEII GGTNACC 1 cut(s) 704
BstF5I GGATG 4 cut(s) 432, 693, 748, 1439
BstFNI CGCG 2 cut(s) 11, 27
BstH2I RGCGCY 2 cut(s) 329, 1451
BstHHI GCGC 3 cut(s) 328, 1450, 1534
BstKTI GATC 4 cut(s) 148, 271, 1105, 1320
BstMAI GTCTC 6 cut(s) 110, 173, 194, 526, 785, 1581
BstMBI GATC 4 cut(s) 145, 268, 1102, 1317
BstMWI GCNNNNNNNGC 5 cut(s) 36, 255, 877, 1445, 1538
BstNI CCWGG 3 cut(s) 411, 936, 1589
BstPI GGTNACC 1 cut(s) 704
BstSCI CCNGG 4 cut(s) 328, 409, 934, 1587
BstSFI CTRYAG 3 cut(s) 261, 1122, 1514
BstUI CGCG 2 cut(s) 11, 27
BstV1I GCAGC 6 cut(s) 66, 79, 805, 1108, 1130, 1528
BstV2I GAAGAC 1 cut(s) 62
BstX2I RGATCY 2 cut(s) 145, 1102
BstXI CCANNNNNNTGG 1 cut(s) 575
BstYI RGATCY 2 cut(s) 145, 1102
Bsu36I CCTNAGG 1 cut(s) 738
BsuI GTATCC 1 cut(s) 1370
BsuRI GGCC 3 cut(s) 567, 758, 899
BtrI CACGTC 1 cut(s) 1480
BtsCI GGATG 4 cut(s) 432, 693, 748, 1439
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 3 cut(s) 400, 1195, 1227
Cac8I GCNNGC 5 cut(s) 653, 657, 873, 1138, 1539
CfoI GCGC 3 cut(s) 328, 1450, 1534
Cfr13I GGNCC 4 cut(s) 565, 777, 1127, 1523
CseI GACGC 2 cut(s) 28, 211
Csp6I GTAC 2 cut(s) 712, 968
CviAII CATG 6 cut(s) 60, 437, 529, 971, 1326, 1654
CviQI GTAC 2 cut(s) 712, 968
DdeI CTNAG 8 cut(s) 291, 354, 366, 560, 644, 738, 781, 1003
DpnI GATC 4 cut(s) 147, 270, 1104, 1319
DpnII GATC 4 cut(s) 145, 268, 1102, 1317
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Ecl136II GAGCTC 3 cut(s) 258, 941, 1375
Eco130I CCWWGG 2 cut(s) 540, 961
Eco24I GRGCYC 3 cut(s) 260, 943, 1377
Eco47I GGWCC 3 cut(s) 777, 1127, 1523
Eco47III AGCGCT 1 cut(s) 1449
Eco53kI GAGCTC 3 cut(s) 258, 941, 1375
Eco57I CTGAAG 3 cut(s) 56, 170, 1563
Eco81I CCTNAGG 1 cut(s) 738
Eco88I CYCGRG 1 cut(s) 788
Eco91I GGTNACC 1 cut(s) 704
EcoICRI GAGCTC 3 cut(s) 258, 941, 1375
EcoO109I RGGNCCY 2 cut(s) 777, 1127
EcoO65I GGTNACC 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 3 cut(s) 409, 934, 1587
EcoT14I CCWWGG 2 cut(s) 540, 961
EcoT22I ATGCAT 1 cut(s) 1219
EcoT38I GRGCYC 3 cut(s) 260, 943, 1377
ErhI CCWWGG 2 cut(s) 540, 961
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 6 cut(s) 63, 440, 532, 974, 1329, 1657
FaqI GGGAC 1 cut(s) 993
FatI CATG 6 cut(s) 59, 436, 528, 970, 1325, 1653
FauI CCCGC 1 cut(s) 1360
FauNDI CATATG 1 cut(s) 613
Fnu4HI GCNGC 8 cut(s) 28, 55, 93, 819, 1119, 1122, 1517, 1535
FokI GGATG 4 cut(s) 419, 700, 755, 1426
FriOI GRGCYC 3 cut(s) 260, 943, 1377
Fsp4HI GCNGC 8 cut(s) 28, 55, 93, 819, 1119, 1122, 1517, 1535
FspBI CTAG 3 cut(s) 18, 1148, 1268
GlaI GCGC 3 cut(s) 327, 1449, 1533
GluI GCNGC 8 cut(s) 28, 55, 93, 819, 1119, 1122, 1517, 1535
HaeII RGCGCY 2 cut(s) 329, 1451
HaeIII GGCC 3 cut(s) 567, 758, 899
HapII CCGG 1 cut(s) 329
HgaI GACGC 2 cut(s) 28, 211
HhaI GCGC 3 cut(s) 328, 1450, 1534
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 6 cut(s) 63, 440, 532, 974, 1329, 1657
Hin6I GCGC 3 cut(s) 326, 1448, 1532
HinP1I GCGC 3 cut(s) 326, 1448, 1532
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HindIII AAGCTT 3 cut(s) 152, 716, 1013
HinfI GANTC 8 cut(s) 4, 524, 534, 733, 1074, 1156, 1232, 1584
HpaII CCGG 1 cut(s) 329
HphI GGTGA 4 cut(s) 386, 716, 940, 1283
Hpy166II GTNNAC 4 cut(s) 309, 503, 849, 1523
Hpy188I TCNGA 4 cut(s) 150, 170, 886, 1503
Hpy188III TCNNGA 6 cut(s) 132, 368, 730, 737, 806, 1229
Hpy8I GTNNAC 4 cut(s) 309, 503, 849, 1523
Hpy99I CGWCG 1 cut(s) 1481
HpyCH4III ACNGT 6 cut(s) 306, 313, 374, 918, 1358, 1555
HpyCH4IV ACGT 1 cut(s) 1479
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 255, 877, 1445, 1538
HpyF3I CTNAG 8 cut(s) 291, 354, 366, 560, 644, 738, 781, 1003
HpySE526I ACGT 1 cut(s) 1479
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 6 cut(s) 63, 440, 532, 974, 1329, 1657
HspAI GCGC 3 cut(s) 326, 1448, 1532
Kzo9I GATC 4 cut(s) 145, 268, 1102, 1317
LmnI GCTCC 3 cut(s) 938, 1266, 1372
Lsp1109I GCAGC 6 cut(s) 66, 79, 805, 1108, 1130, 1528
LweI GCATC 4 cut(s) 1204, 1226, 1264, 1448
MaeI CTAG 3 cut(s) 18, 1148, 1268
MaeII ACGT 1 cut(s) 1479
MaeIII GTNAC 6 cut(s) 193, 595, 665, 704, 946, 1190
MalI GATC 4 cut(s) 147, 270, 1104, 1319
MbiI CCGCTC 1 cut(s) 953
MboI GATC 4 cut(s) 145, 268, 1102, 1317
MboII GAAGA 9 cut(s) 62, 151, 398, 583, 685, 1112, 1422, 1501, 1556
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 2 cut(s) 145, 1102
MhlI GDGCHC 3 cut(s) 260, 943, 1377
MluCI AATT 7 cut(s) 31, 109, 282, 298, 380, 515, 697
MlyI GAGTC 2 cut(s) 528, 1241
MmeI TCCRAC 1 cut(s) 909
Mph1103I ATGCAT 1 cut(s) 1219
MseI TTAA 3 cut(s) 920, 1305, 1353
MspA1I CMGCKG 3 cut(s) 800, 1121, 1537
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 4 cut(s) 330, 411, 936, 1589
MunI CAATTG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 1484
MvaI CCWGG 3 cut(s) 411, 936, 1589
MvnI CGCG 2 cut(s) 11, 27
MwoI GCNNNNNNNGC 5 cut(s) 36, 255, 877, 1445, 1538
NciI CCSGG 1 cut(s) 330
NdeI CATATG 1 cut(s) 613
NdeII GATC 4 cut(s) 145, 268, 1102, 1317
NlaIII CATG 6 cut(s) 63, 440, 532, 974, 1329, 1657
NlaIV GGNNCC 3 cut(s) 213, 566, 1557
NmuCI GTSAC 4 cut(s) 193, 704, 946, 1190
NsiI ATGCAT 1 cut(s) 1219
PcsI WCGNNNNNNNCGW 1 cut(s) 1200
PctI GAATGC 1 cut(s) 1484
PfeI GAWTC 6 cut(s) 4, 524, 733, 1074, 1156, 1584
PkrI GCNGC 8 cut(s) 29, 56, 94, 820, 1120, 1123, 1518, 1536
PleI GAGTC 2 cut(s) 528, 1240
PpsI GAGTC 2 cut(s) 528, 1240
PpuMI RGGWCCY 2 cut(s) 777, 1127
Psp124BI GAGCTC 3 cut(s) 260, 943, 1377
Psp5II RGGWCCY 2 cut(s) 777, 1127
Psp6I CCWGG 3 cut(s) 409, 934, 1587
PspEI GGTNACC 1 cut(s) 704
PspGI CCWGG 3 cut(s) 409, 934, 1587
PspN4I GGNNCC 3 cut(s) 213, 566, 1557
PspPI GGNCC 4 cut(s) 565, 777, 1127, 1523
PspPPI RGGWCCY 2 cut(s) 777, 1127
PsrI GAACNNNNNNTAC 2 cut(s) 495, 527
PstI CTGCAG 3 cut(s) 265, 1126, 1518
PsuI RGATCY 2 cut(s) 145, 1102
PvuII CAGCTG 2 cut(s) 800, 1121
RsaI GTAC 2 cut(s) 713, 969
RsaNI GTAC 2 cut(s) 712, 968
SacI GAGCTC 3 cut(s) 260, 943, 1377
SaqAI TTAA 3 cut(s) 920, 1305, 1353
SatI GCNGC 8 cut(s) 28, 55, 93, 819, 1119, 1122, 1517, 1535
Sau3AI GATC 4 cut(s) 145, 268, 1102, 1317
Sau96I GGNCC 4 cut(s) 565, 777, 1127, 1523
SchI GAGTC 2 cut(s) 528, 1241
ScrFI CCNGG 4 cut(s) 330, 411, 936, 1589
SduI GDGCHC 3 cut(s) 260, 943, 1377
SfaNI GCATC 4 cut(s) 1204, 1226, 1264, 1448
SfcI CTRYAG 3 cut(s) 261, 1122, 1514
SinI GGWCC 3 cut(s) 777, 1127, 1523
Sse9I AATT 7 cut(s) 31, 109, 282, 298, 380, 515, 697
SsiI CCGC 6 cut(s) 11, 27, 951, 1367, 1419, 1535
SspMI CTAG 3 cut(s) 18, 1148, 1268
SstI GAGCTC 3 cut(s) 260, 943, 1377
StyD4I CCNGG 4 cut(s) 328, 409, 934, 1587
StyI CCWWGG 2 cut(s) 540, 961
TaaI ACNGT 6 cut(s) 306, 313, 374, 918, 1358, 1555
TaiI ACGT 1 cut(s) 1482
TaqI TCGA 2 cut(s) 133, 1377
TaqII GACCGA 1 cut(s) 1540
TasI AATT 7 cut(s) 31, 109, 282, 298, 380, 515, 697
TatI WGTACW 2 cut(s) 711, 967
TauI GCSGC 2 cut(s) 30, 1537
TfiI GAWTC 6 cut(s) 4, 524, 733, 1074, 1156, 1584
Tru1I TTAA 3 cut(s) 920, 1305, 1353
Tru9I TTAA 3 cut(s) 920, 1305, 1353
TscAI CASTG 3 cut(s) 400, 1195, 1227
TseFI GTSAC 4 cut(s) 193, 704, 946, 1190
TseI GCWGC 6 cut(s) 54, 92, 818, 1118, 1121, 1516
Tsp45I GTSAC 4 cut(s) 193, 704, 946, 1190
TspDTI ATGAA 5 cut(s) 17, 336, 475, 987, 1662
TspGWI ACGGA 3 cut(s) 212, 224, 851
TspRI CASTG 3 cut(s) 400, 1195, 1227
VpaK11BI GGWCC 3 cut(s) 777, 1127, 1523
XapI RAATTY 2 cut(s) 282, 298
XcmI CCANNNNNNNNNTGG 2 cut(s) 1317, 1450
XspI CTAG 3 cut(s) 18, 1148, 1268
Zsp2I ATGCAT 1 cut(s) 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.