pycom07g19840

zinc metalloprotease EGY2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008714
Physical Location & Seq
Forward (+)
22102898 .. 22106327
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19840.1

Sequence Viewer

Length: 1488 bp
ATGAACTTCTCCGCAACCTTTCGGGGGAATTTCGTCCCCTTATCGCAGTGCAGCTCTTGCTGCGATCTTCGTTTTCAGCCCTATTTCGCTTCGGGAACCTGTTCTTGGCAAAAACGACATCGTCGTAGAAGCAGCTTGAAGCTCCACCAGGTTTCGAGATTTTTGAGGAAGCGGGAGGTTGTTTGTAGAGTGCGTGAGACGGAGACCGAACCGCCACCGGATAGCAATAACGATGAGGAAAAACAAGGACATGAAAATGAAGATGGAGATTCCGATGCCGTCCATCTCGCTTCGCAGCCGATAGTTGAAGACCAAGTAAACAATGATGCTGAAACCAAGGATCAACAGGAAGTTGAAAATGTAGAAGTTTCTAGTGGATCACCTCTTCCAGGTGTGAAGCCACAGCTGGGTGAATCCATCCGGATTCCAAAGGAAACAATTGATATTCTTAAGAACCAAGTTTTCGGCTTTGATACCTTTTTCGTGACAGGCCAGGATCCATATGAGACTGGAGTATTGTTTAAAGGAAACCTGCGAGGAGTGCCTGCTAAAAGTTATGAAAAGATATCAAAGAGAATGCAGGACAAGTTTGGCGATGAATATAAGCTTTTCCTTCTAGTCAATCCTGAGGATGATCAACCAGTGGCAGTTGTAGTTCCACGAAGGACATTACAGCCAGAGTCAACTGCCGTCCCAGAGTGGTTTGCAGCTGGTGCTTTTGGATTGGTCACGTTGTTTACCCTACTTCTTCAAAATGTTCCTGAATTGCAGTCCAATTTTCTGTCAGCTTTTGATAATCCTGAGCTCTTAAAGAATGGTTTACCTGGAGCCCTTGTAACTGCAGCTGTCCTGGGGGTGCATGAGCTCGGCCATGTTTTAGTTGCAAACAGCACTGGAGTCGTGCTTGGAGTGCCATACTTTATCCCCAGTTGGCAGATAGGTTCTTTTGGTACAATTACAAGAATTGTGAATATTGTACCCAAGCGTGAAAGTCTTCTGAAAGTTGCGGCGGCAGGACCCTTGGCTGGATTTTCCTTGGGATTTGTTCTTCTGCTTCTAGGATTCTTCTTACCACCTAGTGATGGCATTGGTGTCGTTGTCGATGCTTCTGTTTTTCACGAGTCTTTTCTTATTGGGGGTACTGCAAAGCTTCTTCTCGGGGAAGCGCTGAAGGAAGGCACTCCCATCTCTCTTAACCCTCTCTTGTTATGGGCTTGGGCTGGACTCGTTATCAACGCTATTAACAGCATCCCAGCTGGAGAGCTTGACGGGGGCAGAATTTCGTTTGCCATTTGGGGAAGAAAGGCTGCAACTCGCATCACCGGTGCTTCAATCGCGCTGCTCGGATTATCATCCCTAGTCAGCGACGTGGCATTTTATTGGGTGGTTTTGATATTTTTCTTACAGAGAGGGCCGATTGCTCCACTGTACGAGGAGATCTTGACATTGATGATCCTGACGACAAGTACGTTGGCCTCGGGGTATTAG

Protein Analysis

496

Amino Acids

53.97

Weight (kDa)

5.36

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50 PF02163 277 - 436 2.5e-11 Peptidase family M50
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 540
AccII CGCG 1 cut(s) 1337
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 5 cut(s) 12, 172, 212, 1007, 1010
AclWI GGATC 5 cut(s) 348, 385, 491, 504, 1447
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 2 cut(s) 28, 1278
AcuI CTGAAG 1 cut(s) 1190
AdeI CACNNNGTG 1 cut(s) 1079
AfaI GTAC 5 cut(s) 952, 978, 1141, 1430, 1468
AfeI AGCGCT 1 cut(s) 1167
AfiI CCNNNNNNNGG 6 cut(s) 24, 105, 389, 407, 1025, 1082
AflII CTTAAG 1 cut(s) 449
AgeI ACCGGT 1 cut(s) 1322
AgsI TTSAA 5 cut(s) 139, 308, 356, 752, 1332
AjiI CACGTC 1 cut(s) 1369
AjnI CCWGG 5 cut(s) 147, 388, 492, 823, 849
AjuI GAANNNNNNNTTGG 2 cut(s) 88, 120
Alw21I GWGCWC 2 cut(s) 807, 867
Alw26I GTCTC 3 cut(s) 191, 197, 500
AlwI GGATC 5 cut(s) 348, 385, 491, 504, 1447
Ama87I CYCGRG 2 cut(s) 1157, 1477
Aor13HI TCCGGA 1 cut(s) 420
Aor51HI AGCGCT 1 cut(s) 1167
AoxI GGCC 4 cut(s) 490, 868, 1412, 1473
ApeKI GCWGC 8 cut(s) 51, 60, 132, 295, 707, 842, 1307, 1339
ApoI RAATTY 2 cut(s) 28, 1278
AsiGI ACCGGT 1 cut(s) 1322
Asp700I GAANNNNTTC 2 cut(s) 100, 993
AspLEI GCGC 2 cut(s) 1168, 1339
AspS9I GGNCC 2 cut(s) 1016, 1412
AsuHPI GGTGA 3 cut(s) 372, 422, 1312
AvaI CYCGRG 2 cut(s) 1157, 1477
AvaII GGWCC 1 cut(s) 1016
AxyI CCTNAGG 1 cut(s) 627
BamHI GGATCC 1 cut(s) 496
BanII GRGCYC 3 cut(s) 807, 832, 867
BauI CACGAG 1 cut(s) 1118
BbsI GAAGAC 2 cut(s) 315, 986
Bbv12I GWGCWC 2 cut(s) 807, 867
BbvI GCAGC 8 cut(s) 47, 63, 144, 307, 719, 854, 1294, 1326
BccI CCATC 5 cut(s) 257, 291, 425, 1076, 1193
BceAI ACGGC 2 cut(s) 263, 674
BcgI CGANNNNNNTGC 4 cut(s) 880, 914, 1075, 1109
BciT130I CCWGG 5 cut(s) 149, 390, 494, 825, 851
BclI TGATCA 1 cut(s) 634
BcoDI GTCTC 3 cut(s) 191, 197, 500
BfaI CTAG 5 cut(s) 372, 617, 1058, 1077, 1358
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 1 cut(s) 1169
BfrI CTTAAG 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 540
BglII AGATCT 1 cut(s) 1437
Bme1390I CCNGG 5 cut(s) 149, 390, 494, 825, 851
Bme18I GGWCC 1 cut(s) 1016
BmeT110I CYCGRG 2 cut(s) 1157, 1477
BmgBI CACGTC 1 cut(s) 1369
BmgT120I GGNCC 2 cut(s) 1016, 1412
BmiI GGNNCC 4 cut(s) 97, 498, 829, 1018
BmrFI CCNGG 5 cut(s) 149, 390, 494, 825, 851
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 5 cut(s) 265, 316, 1093, 1257, 1326
BmuI ACTGGG 1 cut(s) 921
BpiI GAAGAC 2 cut(s) 315, 986
BpmI CTGGAG 4 cut(s) 531, 846, 915, 1278
Bpu10I CCTNAGC 1 cut(s) 801
BsaBI GATNNNNATC 1 cut(s) 1351
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 5 cut(s) 336, 850, 1020, 1035, 1476
BsaWI WCCGGW 3 cut(s) 217, 420, 1322
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 6 cut(s) 24, 105, 389, 407, 1025, 1082
Bse118I RCCGGY 1 cut(s) 1322
Bse1I ACTGG 4 cut(s) 514, 641, 898, 927
Bse21I CCTNAGG 1 cut(s) 627
Bse8I GATNNNNATC 1 cut(s) 1351
BseAI TCCGGA 1 cut(s) 420
BseBI CCWGG 5 cut(s) 149, 390, 494, 825, 851
BseDI CCNNGG 5 cut(s) 336, 850, 1020, 1035, 1476
BseGI GGATG 4 cut(s) 417, 637, 1248, 1352
BseJI GATNNNNATC 1 cut(s) 1351
BseLI CCNNNNNNNGG 6 cut(s) 24, 105, 389, 407, 1025, 1082
BseMII CTCAG 2 cut(s) 618, 792
BseNI ACTGG 4 cut(s) 514, 641, 898, 927
BseRI GAGGAG 2 cut(s) 552, 1448
BseXI GCAGC 8 cut(s) 47, 63, 144, 307, 719, 854, 1294, 1326
BseYI CCCAGC 2 cut(s) 406, 1252
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 1337
BshFI GGCC 4 cut(s) 492, 870, 1414, 1475
BshTI ACCGGT 1 cut(s) 1322
BsiHKAI GWGCWC 2 cut(s) 807, 867
BsiHKCI CYCGRG 2 cut(s) 1157, 1477
BsiSI CCGG 3 cut(s) 218, 421, 1323
BslFI GGGAC 2 cut(s) 20, 677
BslI CCNNNNNNNGG 6 cut(s) 24, 105, 389, 407, 1025, 1082
BsmAI GTCTC 3 cut(s) 191, 197, 500
BsmBI CGTCTC 1 cut(s) 191
BsmFI GGGAC 2 cut(s) 20, 677
BsmI GAATGC 1 cut(s) 582
BsnI GGCC 4 cut(s) 492, 870, 1414, 1475
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 2 cut(s) 1157, 1477
Bsp1286I GDGCHC 3 cut(s) 807, 832, 867
Bsp13I TCCGGA 1 cut(s) 420
Bsp143I GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
BspACI CCGC 5 cut(s) 12, 172, 212, 1007, 1010
BspANI GGCC 4 cut(s) 492, 870, 1414, 1475
BspCNI CTCAG 2 cut(s) 619, 793
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 1 cut(s) 1337
BspLI GGNNCC 4 cut(s) 97, 498, 829, 1018
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 5 cut(s) 348, 385, 491, 504, 1447
BspTI CTTAAG 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 1322
BsrI ACTGG 4 cut(s) 514, 641, 898, 927
BssAI RCCGGY 1 cut(s) 1322
BssECI CCNNGG 5 cut(s) 336, 850, 1020, 1035, 1476
BssMI GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
BssSI CACGAG 1 cut(s) 1118
BssT1I CCWWGG 3 cut(s) 336, 1020, 1035
Bst2BI CACGAG 1 cut(s) 1118
Bst2UI CCWGG 5 cut(s) 149, 390, 494, 825, 851
Bst4CI ACNGT 1 cut(s) 1428
Bst6I CTCTTC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 449
BstAPI GCANNNNNTGC 2 cut(s) 57, 713
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 2 cut(s) 627, 801
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 417, 637, 1248, 1352
BstFNI CGCG 1 cut(s) 1337
BstH2I RGCGCY 1 cut(s) 1169
BstHHI GCGC 2 cut(s) 1168, 1339
BstKTI GATC 7 cut(s) 67, 343, 380, 499, 637, 1440, 1455
BstMAI GTCTC 3 cut(s) 191, 197, 500
BstMBI GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
BstMWI GCNNNNNNNGC 6 cut(s) 57, 60, 541, 713, 910, 1334
BstNI CCWGG 5 cut(s) 149, 390, 494, 825, 851
BstSCI CCNGG 5 cut(s) 147, 388, 492, 823, 849
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 1 cut(s) 1337
BstV1I GCAGC 8 cut(s) 47, 63, 144, 307, 719, 854, 1294, 1326
BstV2I GAAGAC 2 cut(s) 315, 986
BstX2I RGATCY 2 cut(s) 496, 1437
BstYI RGATCY 2 cut(s) 496, 1437
Bsu36I CCTNAGG 1 cut(s) 627
BsuRI GGCC 4 cut(s) 492, 870, 1414, 1475
BtgZI GCGATG 1 cut(s) 609
BtrI CACGTC 1 cut(s) 1369
BtsCI GGATG 4 cut(s) 417, 637, 1248, 1352
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 4 cut(s) 53, 648, 891, 1424
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 1 cut(s) 546
CfoI GCGC 2 cut(s) 1168, 1339
Cfr10I RCCGGY 1 cut(s) 1322
Cfr13I GGNCC 2 cut(s) 1016, 1412
CsiI ACCWGGT 1 cut(s) 147
Csp6I GTAC 5 cut(s) 951, 977, 1140, 1429, 1467
CspAI ACCGGT 1 cut(s) 1322
CviAII CATG 3 cut(s) 251, 860, 872
CviQI GTAC 5 cut(s) 951, 977, 1140, 1429, 1467
DdeI CTNAG 2 cut(s) 627, 801
DpnI GATC 7 cut(s) 66, 342, 379, 498, 636, 1439, 1454
DpnII GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
DraI TTTAAA 1 cut(s) 523
DraIII CACNNNGTG 1 cut(s) 1079
EaeI YGGCCR 1 cut(s) 868
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Ecl136II GAGCTC 2 cut(s) 805, 865
Eco130I CCWWGG 3 cut(s) 336, 1020, 1035
Eco24I GRGCYC 3 cut(s) 807, 832, 867
Eco31I GGTCTC 1 cut(s) 197
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 1016
Eco47III AGCGCT 1 cut(s) 1167
Eco53kI GAGCTC 2 cut(s) 805, 865
Eco57I CTGAAG 1 cut(s) 1190
Eco81I CCTNAGG 1 cut(s) 627
Eco88I CYCGRG 2 cut(s) 1157, 1477
EcoICRI GAGCTC 2 cut(s) 805, 865
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 1016
EcoRII CCWGG 5 cut(s) 147, 388, 492, 823, 849
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 3 cut(s) 336, 1020, 1035
EcoT38I GRGCYC 3 cut(s) 807, 832, 867
ErhI CCWWGG 3 cut(s) 336, 1020, 1035
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 254, 863, 875
FaiI YATR 9 cut(s) 252, 502, 504, 558, 603, 861, 873, 916, 1210
FaqI GGGAC 2 cut(s) 20, 677
FatI CATG 3 cut(s) 250, 859, 871
FauI CCCGC 1 cut(s) 165
FauNDI CATATG 1 cut(s) 502
FbaI TGATCA 1 cut(s) 634
FokI GGATG 4 cut(s) 404, 644, 1235, 1339
FriOI GRGCYC 3 cut(s) 807, 832, 867
FspBI CTAG 5 cut(s) 372, 617, 1058, 1077, 1358
GlaI GCGC 2 cut(s) 1167, 1338
GsaI CCCAGC 2 cut(s) 410, 1256
GsuI CTGGAG 4 cut(s) 531, 846, 915, 1278
HaeII RGCGCY 1 cut(s) 1169
HaeIII GGCC 4 cut(s) 492, 870, 1414, 1475
HapII CCGG 3 cut(s) 218, 421, 1323
HhaI GCGC 2 cut(s) 1168, 1339
Hin1II CATG 3 cut(s) 254, 863, 875
Hin6I GCGC 2 cut(s) 1166, 1337
HinP1I GCGC 2 cut(s) 1166, 1337
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HindIII AAGCTT 2 cut(s) 605, 1148
HinfI GANTC 8 cut(s) 269, 413, 424, 680, 897, 1062, 1121, 1224
HpaII CCGG 3 cut(s) 218, 421, 1323
HphI GGTGA 3 cut(s) 372, 422, 1312
Hpy166II GTNNAC 4 cut(s) 319, 684, 738, 821
Hpy188I TCNGA 3 cut(s) 274, 999, 1346
Hpy8I GTNNAC 4 cut(s) 319, 684, 738, 821
Hpy99I CGWCG 2 cut(s) 126, 1370
HpyAV CCTTC 4 cut(s) 623, 657, 1165, 1169
HpyCH4III ACNGT 1 cut(s) 1428
HpyCH4IV ACGT 3 cut(s) 731, 1368, 1469
HpyCH4V TGCA 9 cut(s) 51, 580, 707, 769, 842, 859, 884, 1145, 1310
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 60, 541, 713, 910, 1334
HpyF3I CTNAG 2 cut(s) 627, 801
HpySE526I ACGT 3 cut(s) 731, 1368, 1469
Hsp92II CATG 3 cut(s) 254, 863, 875
HspAI GCGC 2 cut(s) 1166, 1337
Kpn2I TCCGGA 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
LmnI GCTCC 3 cut(s) 147, 827, 1426
Lsp1109I GCAGC 8 cut(s) 47, 63, 144, 307, 719, 854, 1294, 1326
LweI GCATC 5 cut(s) 265, 316, 1093, 1257, 1326
MabI ACCWGGT 1 cut(s) 147
MaeI CTAG 5 cut(s) 372, 617, 1058, 1077, 1358
MaeII ACGT 3 cut(s) 731, 1368, 1469
MaeIII GTNAC 3 cut(s) 484, 727, 835
MalI GATC 7 cut(s) 66, 342, 379, 498, 636, 1439, 1454
MboI GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 2 cut(s) 496, 1437
MhlI GDGCHC 3 cut(s) 807, 832, 867
MluCI AATT 7 cut(s) 28, 438, 764, 775, 954, 963, 1278
MlyI GAGTC 4 cut(s) 689, 906, 1130, 1218
MroI TCCGGA 1 cut(s) 420
MroXI GAANNNNTTC 2 cut(s) 100, 993
MseI TTAA 5 cut(s) 450, 522, 809, 1194, 1242
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 4 cut(s) 406, 710, 845, 1256
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 3 cut(s) 218, 421, 1323
MspR9I CCNGG 5 cut(s) 149, 390, 494, 825, 851
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 582
MvaI CCWGG 5 cut(s) 149, 390, 494, 825, 851
MvnI CGCG 1 cut(s) 1337
MwoI GCNNNNNNNGC 6 cut(s) 57, 60, 541, 713, 910, 1334
NdeI CATATG 1 cut(s) 502
NdeII GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
NlaIII CATG 3 cut(s) 254, 863, 875
NlaIV GGNNCC 4 cut(s) 97, 498, 829, 1018
NmeAIII GCCGAG 1 cut(s) 846
NmuCI GTSAC 2 cut(s) 484, 727
PcsI WCGNNNNNNNCGW 2 cut(s) 121, 1466
PctI GAATGC 1 cut(s) 582
PdmI GAANNNNTTC 2 cut(s) 100, 993
PfeI GAWTC 4 cut(s) 269, 413, 424, 1062
PflFI GACNNNGTC 1 cut(s) 120
PinAI ACCGGT 1 cut(s) 1322
PleI GAGTC 4 cut(s) 688, 905, 1129, 1218
PpsI GAGTC 4 cut(s) 688, 905, 1129, 1218
PpuMI RGGWCCY 1 cut(s) 1016
Psp124BI GAGCTC 2 cut(s) 807, 867
Psp5II RGGWCCY 1 cut(s) 1016
Psp6I CCWGG 5 cut(s) 147, 388, 492, 823, 849
PspFI CCCAGC 2 cut(s) 406, 1252
PspGI CCWGG 5 cut(s) 147, 388, 492, 823, 849
PspN4I GGNNCC 4 cut(s) 97, 498, 829, 1018
PspPI GGNCC 2 cut(s) 1016, 1412
PspPPI RGGWCCY 1 cut(s) 1016
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 2 cut(s) 496, 1437
PsyI GACNNNGTC 1 cut(s) 120
PvuII CAGCTG 4 cut(s) 406, 710, 845, 1256
RsaI GTAC 5 cut(s) 952, 978, 1141, 1430, 1468
RsaNI GTAC 5 cut(s) 951, 977, 1140, 1429, 1467
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 2 cut(s) 807, 867
SaqAI TTAA 5 cut(s) 450, 522, 809, 1194, 1242
Sau3AI GATC 7 cut(s) 64, 340, 377, 496, 634, 1437, 1452
Sau96I GGNCC 2 cut(s) 1016, 1412
SchI GAGTC 4 cut(s) 689, 906, 1130, 1218
ScrFI CCNGG 5 cut(s) 149, 390, 494, 825, 851
SduI GDGCHC 3 cut(s) 807, 832, 867
SexAI ACCWGGT 1 cut(s) 147
SfaNI GCATC 5 cut(s) 265, 316, 1093, 1257, 1326
SfcI CTRYAG 1 cut(s) 840
SgrAI CRCCGGYG 1 cut(s) 1322
SinI GGWCC 1 cut(s) 1016
SmiMI CAYNNNNRTG 1 cut(s) 255
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 7 cut(s) 28, 438, 764, 775, 954, 963, 1278
SsiI CCGC 5 cut(s) 12, 172, 212, 1007, 1010
SspI AATATT 1 cut(s) 973
SspMI CTAG 5 cut(s) 372, 617, 1058, 1077, 1358
SstI GAGCTC 2 cut(s) 807, 867
StyD4I CCNGG 5 cut(s) 147, 388, 492, 823, 849
StyI CCWWGG 3 cut(s) 336, 1020, 1035
TaaI ACNGT 1 cut(s) 1428
TaiI ACGT 3 cut(s) 734, 1371, 1472
TaqI TCGA 2 cut(s) 155, 1101
TaqII GACCGA 1 cut(s) 221
TasI AATT 7 cut(s) 28, 438, 764, 775, 954, 963, 1278
TauI GCSGC 2 cut(s) 1010, 1013
TfiI GAWTC 4 cut(s) 269, 413, 424, 1062
Tru1I TTAA 5 cut(s) 450, 522, 809, 1194, 1242
Tru9I TTAA 5 cut(s) 450, 522, 809, 1194, 1242
TscAI CASTG 4 cut(s) 53, 648, 898, 1431
TseFI GTSAC 2 cut(s) 484, 727
TseI GCWGC 8 cut(s) 51, 60, 132, 295, 707, 842, 1307, 1339
Tsp45I GTSAC 2 cut(s) 484, 727
TspDTI ATGAA 5 cut(s) 17, 267, 273, 573, 612
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 4 cut(s) 53, 648, 898, 1431
Tth111I GACNNNGTC 1 cut(s) 120
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 1016
XagI CCTNNNNNAGG 1 cut(s) 387
XapI RAATTY 2 cut(s) 28, 1278
XmnI GAANNNNTTC 2 cut(s) 100, 993
XspI CTAG 5 cut(s) 372, 617, 1058, 1077, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.