AT5G05930

Guanylylate cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004784
Physical Location & Seq
Reverse (-)
1779870 .. 1783465
3596 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05930.3

Sequence Viewer

Length: 825 bp
ATGTGGCCTCTTTGTTTTCTGCTTAACAAGCTATTAAGAGTTGAGGAGAGGAATCAGGGGATATTAGACGGAAATGGAGATTCGACTTTTCCGAAATACTGCCTATTCGATGATCCCTTGGTGAGTGATGGAAAGTACAGAGATGCAGGGTTGCCATCCTCGTCCCACATGGATGTCCCTCATGTACATCAGTTAGCTTCATGGGATTGCGGTCTCGCTTGTGTTCTTATGGTTCTCAGAGCAAGTGGCATCGCAAGCTGCACTCTTGAGGATTTGGCTGAGATATGCTCCACAAATAGCATTTGGACTGTCGATCTCGCATATTTACTGCAGAAGTTCTGTGTGGAATTTTCATATTACACGATAACATTTGGAGCAAACCCGAATTATTCCATAGAGGAATTTTACAAGGAGCAGCTCCCTGAAGATCTGGTGCGTGTCGATTTGCTCTTCAGGAAAGCACATGAATCTGGCATTATTATACAGTGCAGATCAGTTAGTATCCATGAGATTTCTTGTTTGCTATTGTCGGGGAACTATATTGCCATTGCGTTAGTCGACCAGGACAAGCTAAGCAAGTCTTGGTTGGAGGAAGTGCTTGTCTCTGGTCTTCACTCCAGCAATTCTTGTTACACTGGTCACTATGTCGTGATATGTGGCTATGATGCTGTTAGGGATGAGTTCGAGATCAGAGATCCCGCCAGTTCCAAGATACATGAGAGGATCTCGTCAAAATGCCTGGAGAATGCCCGGAAATCGTTTGGCACAGACGAAGATCTTCTCCTGATAAACTTGGAGAATATGAGGAACCAGAACGGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Guanylate_cyc_2

Protein Analysis

274

Amino Acids

30.91

Weight (kDa)

4.88

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Guanylate_cyc_2 PF09778 59 - 213 9.1e-51 Guanylylate cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 558
AciI CCGC 2 cut(s) 210, 699
AclWI GGATC 3 cut(s) 107, 689, 731
AcsI RAATTY 2 cut(s) 347, 401
AcuI CTGAAG 2 cut(s) 436, 444
AfaI GTAC 2 cut(s) 137, 186
AjnI CCWGG 2 cut(s) 561, 738
AluBI AGCT 5 cut(s) 31, 197, 258, 418, 571
AluI AGCT 5 cut(s) 31, 197, 258, 418, 571
Alw26I GTCTC 2 cut(s) 218, 607
AlwI GGATC 3 cut(s) 107, 689, 731
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 258, 415
ApoI RAATTY 2 cut(s) 347, 401
Asp700I GAANNNNTTC 1 cut(s) 777
AsuC2I CCSGG 1 cut(s) 751
AsuHPI GGTGA 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 602
BbvI GCAGC 2 cut(s) 245, 427
BccI CCATC 2 cut(s) 122, 163
BciT130I CCWGG 2 cut(s) 563, 740
BciVI GTATCC 1 cut(s) 512
BcnI CCSGG 1 cut(s) 751
BcoDI GTCTC 2 cut(s) 218, 607
BfmI CTRYAG 1 cut(s) 329
BfuI GTATCC 1 cut(s) 512
BglII AGATCT 2 cut(s) 427, 775
BisI GCNGC 2 cut(s) 259, 416
BlpI GCTNAGC 1 cut(s) 572
BlsI GCNGC 2 cut(s) 260, 417
Bme1390I CCNGG 3 cut(s) 563, 740, 751
BmiI GGNNCC 1 cut(s) 809
BmrFI CCNGG 3 cut(s) 563, 740, 751
BmsI GCATC 3 cut(s) 133, 258, 655
BpiI GAAGAC 1 cut(s) 602
BplI GAGNNNNNCTC 4 cut(s) 272, 304, 710, 742
BpmI CTGGAG 2 cut(s) 601, 761
Bpu1102I GCTNAGC 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 751
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 640, 702
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 2 cut(s) 563, 740
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 3 cut(s) 155, 178, 682
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 2 cut(s) 250, 270
BseNI ACTGG 2 cut(s) 640, 702
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 245, 427
BsgI GTGCAG 2 cut(s) 244, 508
BshFI GGCC 1 cut(s) 7
BsiSI CCGG 1 cut(s) 751
BslFI GGGAC 2 cut(s) 148, 161
BsmAI GTCTC 2 cut(s) 218, 607
BsmFI GGGAC 2 cut(s) 148, 161
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 218
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
Bsp1720I GCTNAGC 1 cut(s) 572
BspACI CCGC 2 cut(s) 210, 699
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 2 cut(s) 249, 271
BspLI GGNNCC 1 cut(s) 809
BspMAI CTGCAG 1 cut(s) 333
BspPI GGATC 3 cut(s) 107, 689, 731
BspQI GCTCTTC 1 cut(s) 455
BspTNI GGTCTC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 2 cut(s) 640, 702
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 563, 740
Bst4CI ACNGT 2 cut(s) 310, 486
Bst6I CTCTTC 1 cut(s) 455
BstAUI TGTACA 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 3 cut(s) 236, 279, 572
BstF5I GGATG 3 cut(s) 155, 178, 682
BstKTI GATC 8 cut(s) 115, 316, 430, 494, 690, 697, 726, 778
BstMAI GTCTC 2 cut(s) 218, 607
BstMBI GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
BstMWI GCNNNNNNNGC 2 cut(s) 28, 255
BstNI CCWGG 2 cut(s) 563, 740
BstSCI CCNGG 3 cut(s) 561, 738, 749
BstSFI CTRYAG 1 cut(s) 329
BstV1I GCAGC 2 cut(s) 245, 427
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 4 cut(s) 427, 694, 723, 775
BstYI RGATCY 4 cut(s) 427, 694, 723, 775
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 1 cut(s) 7
BtgZI GCGATG 1 cut(s) 235
BtsCI GGATG 3 cut(s) 155, 178, 682
BtsIMutI CAGTG 2 cut(s) 491, 633
Cac8I GCNNGC 1 cut(s) 256
Csp6I GTAC 2 cut(s) 136, 185
CviAII CATG 6 cut(s) 169, 182, 201, 464, 506, 716
CviJI RGCY 8 cut(s) 7, 31, 197, 258, 278, 418, 571, 660
CviKI_1 RGCY 8 cut(s) 7, 31, 197, 258, 278, 418, 571, 660
CviQI GTAC 2 cut(s) 136, 185
DdeI CTNAG 3 cut(s) 236, 279, 572
DpnI GATC 8 cut(s) 114, 315, 429, 493, 689, 696, 725, 777
DpnII GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 436, 444
EcoRII CCWGG 2 cut(s) 561, 738
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 6 cut(s) 172, 185, 204, 467, 509, 719
FalI AAGNNNNNCTT 2 cut(s) 565, 597
FaqI GGGAC 2 cut(s) 148, 161
FatI CATG 6 cut(s) 168, 181, 200, 463, 505, 715
FauI CCCGC 1 cut(s) 706
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 2 cut(s) 259, 416
FokI GGATG 3 cut(s) 142, 185, 689
Fsp4HI GCNGC 2 cut(s) 259, 416
GluI GCNGC 2 cut(s) 259, 416
GsuI CTGGAG 2 cut(s) 601, 761
HaeIII GGCC 1 cut(s) 7
HapII CCGG 1 cut(s) 751
Hin1II CATG 6 cut(s) 172, 185, 204, 467, 509, 719
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 3 cut(s) 52, 80, 467
HpaII CCGG 1 cut(s) 751
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 559
Hpy188I TCNGA 3 cut(s) 93, 239, 692
Hpy188III TCNNGA 5 cut(s) 266, 454, 649, 685, 784
Hpy8I GTNNAC 1 cut(s) 559
HpyCH4III ACNGT 2 cut(s) 310, 486
HpyCH4V TGCA 4 cut(s) 146, 261, 331, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 255
HpyF3I CTNAG 3 cut(s) 236, 279, 572
Hsp92II CATG 6 cut(s) 172, 185, 204, 467, 509, 719
Kzo9I GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 4 cut(s) 293, 374, 412, 423
Lsp1109I GCAGC 2 cut(s) 245, 427
LweI GCATC 3 cut(s) 133, 258, 655
MaeIII GTNAC 2 cut(s) 629, 638
MalI GATC 8 cut(s) 114, 315, 429, 493, 689, 696, 725, 777
MboI GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
MboII GAAGA 5 cut(s) 437, 442, 602, 770, 785
MflI RGATCY 4 cut(s) 427, 694, 723, 775
MluCI AATT 4 cut(s) 347, 385, 401, 622
MmeI TCCRAC 1 cut(s) 567
MroXI GAANNNNTTC 1 cut(s) 777
MseI TTAA 2 cut(s) 24, 35
MslI CAYNNNNRTG 1 cut(s) 171
MspI CCGG 1 cut(s) 751
MspR9I CCNGG 3 cut(s) 563, 740, 751
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 2 cut(s) 563, 740
MwoI GCNNNNNNNGC 2 cut(s) 28, 255
NciI CCSGG 1 cut(s) 751
NdeII GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
NlaIII CATG 6 cut(s) 172, 185, 204, 467, 509, 719
NlaIV GGNNCC 1 cut(s) 809
NmuCI GTSAC 1 cut(s) 638
PciSI GCTCTTC 1 cut(s) 455
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 777
PfeI GAWTC 3 cut(s) 52, 80, 467
PkrI GCNGC 2 cut(s) 260, 417
Psp6I CCWGG 2 cut(s) 561, 738
PspGI CCWGG 2 cut(s) 561, 738
PspN4I GGNNCC 1 cut(s) 809
PstI CTGCAG 1 cut(s) 333
PsuI RGATCY 4 cut(s) 427, 694, 723, 775
RsaI GTAC 2 cut(s) 137, 186
RsaNI GTAC 2 cut(s) 136, 185
RseI CAYNNNNRTG 1 cut(s) 171
SalI GTCGAC 1 cut(s) 557
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 2 cut(s) 24, 35
SatI GCNGC 2 cut(s) 259, 416
Sau3AI GATC 8 cut(s) 112, 313, 427, 491, 687, 694, 723, 775
ScrFI CCNGG 3 cut(s) 563, 740, 751
SetI ASST 5 cut(s) 33, 199, 260, 420, 573
SfaNI GCATC 3 cut(s) 133, 258, 655
SfcI CTRYAG 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 4 cut(s) 347, 385, 401, 622
SsiI CCGC 2 cut(s) 210, 699
StyD4I CCNGG 3 cut(s) 561, 738, 749
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 2 cut(s) 310, 486
TaqI TCGA 6 cut(s) 83, 108, 312, 441, 558, 684
TasI AATT 4 cut(s) 347, 385, 401, 622
TatI WGTACW 2 cut(s) 135, 184
TfiI GAWTC 3 cut(s) 52, 80, 467
Tru1I TTAA 2 cut(s) 24, 35
Tru9I TTAA 2 cut(s) 24, 35
TscAI CASTG 2 cut(s) 491, 640
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 2 cut(s) 258, 415
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 3 cut(s) 189, 342, 480
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 2 cut(s) 491, 640
XapI RAATTY 2 cut(s) 347, 401
XmiI GTMKAC 1 cut(s) 558
XmnI GAANNNNTTC 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.