FvH4_7g23620

Protein GUCD1-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004784
Physical Location & Seq
Forward (+)
18378434 .. 18384154
5721 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23620.t6

Sequence Viewer

Length: 840 bp
ATGTGGCCTACATATCTGCTGTTTAACAAGAGGGAAGATGAAGAAGCATCAGTCGGCAAAAAGTCTGATTTGATAGAATCTTATCCATGTGAACAGTCACTGAGAGATGGCAAATGCCATCTTTCTGGCTTGCCATGCTCGCACTTTGTTGAAGTCCCACACATAAACCAGCTATATTCTTGGGATTGTGGCCTCGCCTGTGTTCTGATGGTATTTAGGACTGTTGGCATTGACAGCTGCGATATTCAGACATTGGCAGAGTTATGTTGCACAAATAGCATCTGGACTGTTGATCTAGCATACTTGTTGCAAAAGTTCTCTATCAGTTTCTCATACTACACGGTAACATTTGGAGCAAACCCAAAATATTCTGGCGAGACATTTTACAAGGAGCACTTACCTAATGATCTGGTGCGAGTTGATACACTATTTCAAAAGGCACTGGAAGCTGGAATTAGGATAAAGTGCAGGTCAGTCAGTCAAGAAGAAATTTGTTTCTTGATTCTGTGTGGAAAATACATTGCCATAGTATTGGTTGACCAATATAAATTGAGCCCATCTAGTCATGACAATGTTATTATCTCAGACCTTTGTGCAAGCAACTCTGATTACACAGGTCATTATGTCATCATATGTGGGTATGACACTGCTACCGATGAGTTTGAGATTAGAGATCCGGCCTGTTCAAGGAAACATGAAAGGGTATCGTCAACATGCTTAGAAGAAGCACGTAAATCCTTTGGTACTGATGAAGACCTTCTCTTGATCTCTTTGAGGAAGAGTAGGAAGCCAGATAGCCCCTCAATACAACACCCCCGGAATGATAATATTAATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Guanylate_cyc_2

Protein Analysis

280

Amino Acids

31.62

Weight (kDa)

5.29

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Guanylate_cyc_2 PF09778 15 - 181 7.5e-59 Guanylylate cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 459
AclWI GGATC 1 cut(s) 668
AcsI RAATTY 1 cut(s) 489
AfaI GTAC 1 cut(s) 745
AfiI CCNNNNNNNGG 1 cut(s) 687
AgsI TTSAA 3 cut(s) 152, 434, 687
AluBI AGCT 3 cut(s) 172, 237, 449
AluI AGCT 3 cut(s) 172, 237, 449
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 1 cut(s) 668
AlwNI CAGNNNCTG 1 cut(s) 100
AoxI GGCC 3 cut(s) 5, 190, 678
ApeKI GCWGC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 489
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 756
AsuC2I CCSGG 1 cut(s) 817
BanII GRGCYC 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 224
BccI CCATC 4 cut(s) 101, 126, 202, 565
BcnI CCSGG 1 cut(s) 817
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 296, 561
BfuAI ACCTGC 1 cut(s) 459
BisI GCNGC 1 cut(s) 238
BlsI GCNGC 1 cut(s) 239
Bme1390I CCNGG 1 cut(s) 817
BmrFI CCNGG 1 cut(s) 817
BmsI GCATC 2 cut(s) 56, 288
BpiI GAAGAC 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 817
BsaAI YACGTR 1 cut(s) 731
BsaJI CCNNGG 1 cut(s) 815
Bsc4I CCNNNNNNNGG 1 cut(s) 687
Bse1I ACTGG 1 cut(s) 447
Bse3DI GCAATG 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 815
BseLI CCNNNNNNNGG 1 cut(s) 687
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 92, 597
BseNI ACTGG 1 cut(s) 447
BseXI GCAGC 1 cut(s) 224
BsgI GTGCAG 1 cut(s) 487
BshFI GGCC 3 cut(s) 7, 192, 680
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 2 cut(s) 677, 817
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 687
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 3 cut(s) 7, 192, 680
Bsp1286I GDGCHC 2 cut(s) 396, 557
Bsp143I GATC 4 cut(s) 292, 406, 673, 765
BspANI GGCC 3 cut(s) 7, 192, 680
BspCNI CTCAG 2 cut(s) 93, 596
BspHI TCATGA 1 cut(s) 565
BspMI ACCTGC 1 cut(s) 459
BspPI GGATC 1 cut(s) 668
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 1 cut(s) 447
BssECI CCNNGG 1 cut(s) 815
BssMI GATC 4 cut(s) 292, 406, 673, 765
Bst4CI ACNGT 4 cut(s) 96, 223, 289, 343
Bst6I CTCTTC 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 131, 140, 598
BstDEI CTNAG 3 cut(s) 101, 583, 718
BstENI CCTNNNNNAGG 1 cut(s) 685
BstKTI GATC 4 cut(s) 295, 409, 676, 768
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 4 cut(s) 292, 406, 673, 765
BstMWI GCNNNNNNNGC 5 cut(s) 135, 139, 234, 276, 446
BstNSI RCATGY 1 cut(s) 717
BstSCI CCNGG 1 cut(s) 815
BstV1I GCAGC 1 cut(s) 224
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 2 cut(s) 125, 532
BstYI RGATCY 1 cut(s) 673
BsuRI GGCC 3 cut(s) 7, 192, 680
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 3 cut(s) 98, 440, 645
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 3 cut(s) 131, 140, 598
CaiI CAGNNNCTG 1 cut(s) 100
CciI TCATGA 1 cut(s) 565
Csp6I GTAC 1 cut(s) 744
CviAII CATG 5 cut(s) 87, 135, 566, 695, 714
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 3 cut(s) 101, 583, 718
DpnI GATC 4 cut(s) 294, 408, 675, 767
DpnII GATC 4 cut(s) 292, 406, 673, 765
Eam1104I CTCTTC 1 cut(s) 773
EarI CTCTTC 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 557
EcoNI CCTNNNNNAGG 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 557
FaeI CATG 5 cut(s) 90, 138, 569, 698, 717
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 140
FatI CATG 5 cut(s) 86, 134, 565, 694, 713
FauNDI CATATG 1 cut(s) 632
Fnu4HI GCNGC 1 cut(s) 238
FriOI GRGCYC 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 238
FspBI CTAG 2 cut(s) 296, 561
GluI GCNGC 1 cut(s) 238
HaeIII GGCC 3 cut(s) 7, 192, 680
HapII CCGG 2 cut(s) 677, 817
Hin1II CATG 5 cut(s) 90, 138, 569, 698, 717
HincII GTYRAC 2 cut(s) 538, 711
HindII GTYRAC 2 cut(s) 538, 711
HinfI GANTC 2 cut(s) 77, 502
HpaII CCGG 2 cut(s) 677, 817
Hpy166II GTNNAC 3 cut(s) 92, 538, 711
Hpy188I TCNGA 5 cut(s) 67, 207, 249, 586, 607
Hpy188III TCNNGA 6 cut(s) 283, 482, 499, 566, 763, 837
Hpy8I GTNNAC 3 cut(s) 92, 538, 711
HpyAV CCTTC 1 cut(s) 767
HpyCH4III ACNGT 4 cut(s) 96, 223, 289, 343
HpyCH4IV ACGT 1 cut(s) 730
HpyCH4V TGCA 4 cut(s) 270, 310, 468, 596
HpyF10VI GCNNNNNNNGC 5 cut(s) 135, 139, 234, 276, 446
HpyF3I CTNAG 3 cut(s) 101, 583, 718
HpySE526I ACGT 1 cut(s) 730
Hsp92II CATG 5 cut(s) 90, 138, 569, 698, 717
Kzo9I GATC 4 cut(s) 292, 406, 673, 765
LmnI GCTCC 2 cut(s) 353, 391
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 2 cut(s) 56, 288
MaeI CTAG 2 cut(s) 296, 561
MaeII ACGT 1 cut(s) 730
MaeIII GTNAC 2 cut(s) 96, 343
MalI GATC 4 cut(s) 294, 408, 675, 767
MboI GATC 4 cut(s) 292, 406, 673, 765
MboII GAAGA 6 cut(s) 47, 53, 497, 734, 764, 790
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 2 cut(s) 396, 557
MluCI AATT 4 cut(s) 453, 489, 548, 832
MnlI CCTC 4 cut(s) 24, 203, 768, 811
MroXI GAANNNNTTC 1 cut(s) 756
MseI TTAA 2 cut(s) 24, 831
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 2 cut(s) 677, 817
MspR9I CCNGG 1 cut(s) 817
MwoI GCNNNNNNNGC 5 cut(s) 135, 139, 234, 276, 446
NciI CCSGG 1 cut(s) 817
NdeI CATATG 1 cut(s) 632
NdeII GATC 4 cut(s) 292, 406, 673, 765
NlaIII CATG 5 cut(s) 90, 138, 569, 698, 717
NmuCI GTSAC 1 cut(s) 96
NspI RCATGY 1 cut(s) 717
PagI TCATGA 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 2 cut(s) 77, 502
PkrI GCNGC 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 731
PshBI ATTAAT 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 237
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
RseI CAYNNNNRTG 1 cut(s) 570
SaqAI TTAA 2 cut(s) 24, 831
SatI GCNGC 1 cut(s) 238
Sau3AI GATC 4 cut(s) 292, 406, 673, 765
ScrFI CCNGG 1 cut(s) 817
SduI GDGCHC 2 cut(s) 396, 557
SetI ASST 9 cut(s) 174, 239, 403, 451, 473, 591, 619, 733, 759
SfaNI GCATC 2 cut(s) 56, 288
SmiMI CAYNNNNRTG 1 cut(s) 570
Sse9I AATT 4 cut(s) 453, 489, 548, 832
SspI AATATT 2 cut(s) 368, 829
SspMI CTAG 2 cut(s) 296, 561
StyD4I CCNGG 1 cut(s) 815
TaaI ACNGT 4 cut(s) 96, 223, 289, 343
TaiI ACGT 1 cut(s) 733
TasI AATT 4 cut(s) 453, 489, 548, 832
TfiI GAWTC 2 cut(s) 77, 502
Tru1I TTAA 2 cut(s) 24, 831
Tru9I TTAA 2 cut(s) 24, 831
TscAI CASTG 3 cut(s) 105, 447, 652
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 237
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 3 cut(s) 54, 711, 765
TspRI CASTG 3 cut(s) 105, 447, 652
VspI ATTAAT 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 685
XapI RAATTY 1 cut(s) 489
XceI RCATGY 1 cut(s) 717
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 2 cut(s) 296, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.