Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004784

Physical Location & Seq

Genomic Coordinates Forward (+)

25154917 .. 25160953

Gene Structure 6037 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1142100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 795 bp

ATGTGGCCTTCGTATTTTTTATTCAACAAGATTCTTAAGACAGAAGATGAAGATGTATCAGATGGAGAACTTTCAGGCATGGTAGAACCCTACCCATGTGAACAGCCGTTGCCCTGCTCGCACTTTGTTGAAGTCCCGCACATAAACCAGCAAGATTCTTGGGATTGCGGCCTTGCCTGTGTTCTGATGGTCTTTCGGACCGTTGGCATAGACAACTGCGATATTCAGACATTGGCACAGCTATGTGGCACAACTAGCATTTGGACTGTTGATCTAGCATTTTTATTACAGACGTTCTCCATTAGTTTTTGCTACTACACAGTGACAGTTGGAGCAAACCCAAGTTATTCAGGGGAGAAATTTTACAAGGAGCAATTACCTAATGATCTGGAGCGAGTCAATAATCTATTTCAAAAAGCACTGGAAGGTGGAATGAATATACAGCGCAGATCAATCAGTCGACAAGAAATTTGTTTCGCAATTTTGTCCGGGAAATACATTGCCATTGTTTTGGTTGATCAGTATAAATTGAGTAGGTCTCGTCTAGACGATGTTTTTGTCTCAGACTTTTGTGGAAGCAACTCTGGTTACACAGGTCACTATGTTGTCATATGTGGGTATGACACTACCACGGATGAGTTTGAAATTAGAGATCCGGCCTGTTCAAGGAAAAACGAGAGGATCTCATCAACATGCTTAGAAGAAGCCCGCAAATCCTTTGGTACTGATGAGGATCTTCTCTTGATATCTCTGAAGAAGAGTGGGAAGCAAAATCGCCCGGCAATACAACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Guanylate_cyc_2

Protein Analysis

265

Amino Acids

29.86

Weight (kDa)

4.79

Isoelectric Point (pI)

44.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C39_2	PF13529	44 - 220	3e-08	Peptidase_C39 like family
Guanylate_cyc_2	PF09778	45 - 249	6.6e-73	Guanylylate cyclase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05930 AT5G05930 AT5G05930 AT5G05930 AT5G05930 AT5G05930 AT5G05930
fragaria_vesca	FvH4_7g23620 FvH4_7g23620 FvH4_7g23620 FvH4_7g23620 FvH4_7g23620 FvH4_7g23620 FvH4_7g23620
malus_domestica	MD01G1142100.v1.1 MD07G1207800.v1.1
prunus_persica	Prupe.2G243100_v2.0.a1 Prupe.2G243100_v2.0.a1
pyrus_communis	pycom01g16230 pycom07g19330
rosa_chinensis	RchiOBHm_Chr1g0368881
rosa_laevigata	RLG00000027180
rosa_multiflora	Rmu_sc0004250.1_g000049
rosa_roxburghii	Rroxscaffold_4G00288240
rosa_rugosa	Rorug01G0345400 Rorug01G0345500
rosa_samantha	Rh1AG352600 Rh1BG316100 Rh1CG330800 Rh1DG347600
rosa_wichuraiana	Rw0G010000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	460
AciI	CCGC	3 cut(s)	137, 168, 709
AclWI	GGATC	3 cut(s)	647, 689, 741
AcsI	RAATTY	2 cut(s)	359, 468
AcuI	CTGAAG	1 cut(s)	773
AfaI	GTAC	1 cut(s)	724
AfiI	CCNNNNNNNGG	1 cut(s)	666
AflII	CTTAAG	1 cut(s)	35
AgsI	TTSAA	5 cut(s)	25, 131, 413, 644, 666
AluBI	AGCT	1 cut(s)	241
AluI	AGCT	1 cut(s)	241
Alw26I	GTCTC	2 cut(s)	543, 565
AlwI	GGATC	3 cut(s)	647, 689, 741
AoxI	GGCC	3 cut(s)	5, 169, 657
ApoI	RAATTY	2 cut(s)	359, 468
ArsI	GACNNNNNNTTYG	4 cut(s)	453, 485, 539, 571
AspLEI	GCGC	1 cut(s)	447
AspS9I	GGNCC	1 cut(s)	198
AsuC2I	CCSGG	2 cut(s)	490, 779
AvaII	GGWCC	1 cut(s)	198
BccI	CCATC	2 cut(s)	56, 181
BceAI	ACGGC	1 cut(s)	91
BclI	TGATCA	1 cut(s)	517
BcnI	CCSGG	2 cut(s)	490, 779
BcoDI	GTCTC	2 cut(s)	543, 565
BfaI	CTAG	3 cut(s)	255, 275, 545
BfrI	CTTAAG	1 cut(s)	35
BisI	GCNGC	1 cut(s)	169
BlsI	GCNGC	1 cut(s)	170
Bme1390I	CCNGG	2 cut(s)	490, 779
Bme18I	GGWCC	1 cut(s)	198
BmgT120I	GGNCC	1 cut(s)	198
BmrFI	CCNGG	2 cut(s)	490, 779
BplI	GAGNNNNNCTC	4 cut(s)	523, 555, 668, 700
BpmI	CTGGAG	1 cut(s)	410
BpuMI	CCSGG	2 cut(s)	490, 779
BsaBI	GATNNNNATC	1 cut(s)	732
BsaI	GGTCTC	1 cut(s)	543
BsaJI	CCNNGG	1 cut(s)	630
Bsc4I	CCNNNNNNNGG	1 cut(s)	666
Bse1I	ACTGG	1 cut(s)	426
Bse3DI	GCAATG	1 cut(s)	498
Bse8I	GATNNNNATC	1 cut(s)	732
BseDI	CCNNGG	1 cut(s)	630
BseGI	GGATG	1 cut(s)	640
BseJI	GATNNNNATC	1 cut(s)	732
BseLI	CCNNNNNNNGG	1 cut(s)	666
BseMI	GCAATG	1 cut(s)	498
BseMII	CTCAG	1 cut(s)	576
BseNI	ACTGG	1 cut(s)	426
BshFI	GGCC	3 cut(s)	7, 171, 659
BsiSI	CCGG	3 cut(s)	489, 656, 779
BslFI	GGGAC	1 cut(s)	119
BslI	CCNNNNNNNGG	1 cut(s)	666
BsmAI	GTCTC	2 cut(s)	543, 565
BsmFI	GGGAC	1 cut(s)	119
BsnI	GGCC	3 cut(s)	7, 171, 659
Bso31I	GGTCTC	1 cut(s)	543
Bsp143I	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
BspACI	CCGC	3 cut(s)	137, 168, 709
BspANI	GGCC	3 cut(s)	7, 171, 659
BspCNI	CTCAG	1 cut(s)	575
BspPI	GGATC	3 cut(s)	647, 689, 741
BspTI	CTTAAG	1 cut(s)	35
BspTNI	GGTCTC	1 cut(s)	543
BsrDI	GCAATG	1 cut(s)	498
BsrI	ACTGG	1 cut(s)	426
BssECI	CCNNGG	1 cut(s)	630
BssMI	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
Bst4CI	ACNGT	5 cut(s)	202, 268, 322, 328, 792
Bst6I	CTCTTC	1 cut(s)	752
BstAFI	CTTAAG	1 cut(s)	35
BstC8I	GCNNGC	2 cut(s)	119, 709
BstDEI	CTNAG	2 cut(s)	562, 697
BstDSI	CCRYGG	1 cut(s)	630
BstENI	CCTNNNNNAGG	1 cut(s)	664
BstF5I	GGATG	1 cut(s)	640
BstHHI	GCGC	1 cut(s)	447
BstKTI	GATC	7 cut(s)	274, 388, 452, 520, 655, 684, 736
BstMAI	GTCTC	2 cut(s)	543, 565
BstMBI	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
BstMWI	GCNNNNNNNGC	2 cut(s)	118, 255
BstNSI	RCATGY	1 cut(s)	696
BstSCI	CCNGG	2 cut(s)	488, 777
BstX2I	RGATCY	3 cut(s)	652, 681, 733
BstXI	CCANNNNNNTGG	1 cut(s)	511
BstYI	RGATCY	3 cut(s)	652, 681, 733
BsuRI	GGCC	3 cut(s)	7, 171, 659
BtgI	CCRYGG	1 cut(s)	630
BtsCI	GGATG	1 cut(s)	640
BtsIMutI	CAGTG	2 cut(s)	327, 419
Cac8I	GCNNGC	2 cut(s)	119, 709
CfoI	GCGC	1 cut(s)	447
Cfr13I	GGNCC	1 cut(s)	198
CpoI	CGGWCCG	1 cut(s)	198
Csp6I	GTAC	1 cut(s)	723
CspI	CGGWCCG	1 cut(s)	198
CviAII	CATG	3 cut(s)	79, 96, 693
CviJI	RGCY	6 cut(s)	7, 106, 171, 241, 659, 707
CviKI_1	RGCY	6 cut(s)	7, 106, 171, 241, 659, 707
CviQI	GTAC	1 cut(s)	723
DdeI	CTNAG	2 cut(s)	562, 697
DpnI	GATC	7 cut(s)	273, 387, 451, 519, 654, 683, 735
DpnII	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
Eam1104I	CTCTTC	1 cut(s)	752
EarI	CTCTTC	1 cut(s)	752
Eco31I	GGTCTC	1 cut(s)	543
Eco32I	GATATC	1 cut(s)	747
Eco47I	GGWCC	1 cut(s)	198
Eco57I	CTGAAG	1 cut(s)	773
EcoNI	CCTNNNNNAGG	1 cut(s)	664
EcoRV	GATATC	1 cut(s)	747
FaeI	CATG	3 cut(s)	82, 99, 696
FaqI	GGGAC	1 cut(s)	119
FatI	CATG	3 cut(s)	78, 95, 692
FauI	CCCGC	2 cut(s)	144, 716
FauNDI	CATATG	1 cut(s)	611
FbaI	TGATCA	1 cut(s)	517
FblI	GTMKAC	1 cut(s)	460
Fnu4HI	GCNGC	1 cut(s)	169
FokI	GGATG	1 cut(s)	647
Fsp4HI	GCNGC	1 cut(s)	169
FspBI	CTAG	3 cut(s)	255, 275, 545
GlaI	GCGC	1 cut(s)	446
GluI	GCNGC	1 cut(s)	169
GsuI	CTGGAG	1 cut(s)	410
HaeIII	GGCC	3 cut(s)	7, 171, 659
HapII	CCGG	3 cut(s)	489, 656, 779
HhaI	GCGC	1 cut(s)	447
Hin1II	CATG	3 cut(s)	82, 99, 696
Hin6I	GCGC	1 cut(s)	445
HinP1I	GCGC	1 cut(s)	445
HincII	GTYRAC	1 cut(s)	461
HindII	GTYRAC	1 cut(s)	461
HinfI	GANTC	3 cut(s)	31, 155, 396
HpaII	CCGG	3 cut(s)	489, 656, 779
Hpy166II	GTNNAC	2 cut(s)	101, 461
Hpy188I	TCNGA	6 cut(s)	61, 186, 198, 228, 565, 753
Hpy188III	TCNNGA	3 cut(s)	389, 545, 742
Hpy8I	GTNNAC	2 cut(s)	101, 461
HpyAV	CCTTC	2 cut(s)	18, 419
HpyCH4III	ACNGT	5 cut(s)	202, 268, 322, 328, 792
HpyCH4IV	ACGT	1 cut(s)	293
HpyF10VI	GCNNNNNNNGC	2 cut(s)	118, 255
HpyF3I	CTNAG	2 cut(s)	562, 697
HpySE526I	ACGT	1 cut(s)	293
Hsp92II	CATG	3 cut(s)	82, 99, 696
HspAI	GCGC	1 cut(s)	445
Ksp22I	TGATCA	1 cut(s)	517
Kzo9I	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
LmnI	GCTCC	3 cut(s)	332, 370, 391
MaeI	CTAG	3 cut(s)	255, 275, 545
MaeII	ACGT	1 cut(s)	293
MaeIII	GTNAC	3 cut(s)	322, 587, 596
MalI	GATC	7 cut(s)	273, 387, 451, 519, 654, 683, 735
MboI	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
MboII	GAAGA	6 cut(s)	56, 62, 713, 728, 766, 769
MflI	RGATCY	3 cut(s)	652, 681, 733
MluCI	AATT	6 cut(s)	359, 374, 468, 480, 527, 645
MlyI	GAGTC	1 cut(s)	405
MmeI	TCCRAC	1 cut(s)	310
MnlI	CCTC	2 cut(s)	672, 724
MseI	TTAA	1 cut(s)	36
MslI	CAYNNNNRTG	2 cut(s)	241, 691
MspCI	CTTAAG	1 cut(s)	35
MspI	CCGG	3 cut(s)	489, 656, 779
MspR9I	CCNGG	2 cut(s)	490, 779
MwoI	GCNNNNNNNGC	2 cut(s)	118, 255
NciI	CCSGG	2 cut(s)	490, 779
NdeI	CATATG	1 cut(s)	611
NdeII	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
NlaIII	CATG	3 cut(s)	82, 99, 696
NmuCI	GTSAC	2 cut(s)	322, 596
NspI	RCATGY	1 cut(s)	696
PfeI	GAWTC	2 cut(s)	31, 155
PfoI	TCCNGGA	1 cut(s)	488
PkrI	GCNGC	1 cut(s)	170
PleI	GAGTC	1 cut(s)	404
PpsI	GAGTC	1 cut(s)	404
PspPI	GGNCC	1 cut(s)	198
PsuI	RGATCY	3 cut(s)	652, 681, 733
RsaI	GTAC	1 cut(s)	724
RsaNI	GTAC	1 cut(s)	723
RseI	CAYNNNNRTG	2 cut(s)	241, 691
Rsr2I	CGGWCCG	1 cut(s)	198
RsrII	CGGWCCG	1 cut(s)	198
SalI	GTCGAC	1 cut(s)	459
SaqAI	TTAA	1 cut(s)	36
SatI	GCNGC	1 cut(s)	169
Sau3AI	GATC	7 cut(s)	271, 385, 449, 517, 652, 681, 733
Sau96I	GGNCC	1 cut(s)	198
SchI	GAGTC	1 cut(s)	405
ScrFI	CCNGG	2 cut(s)	490, 779
SetI	ASST	6 cut(s)	243, 296, 382, 430, 539, 598
SinI	GGWCC	1 cut(s)	198
SmiMI	CAYNNNNRTG	2 cut(s)	241, 691
SmlI	CTYRAG	1 cut(s)	35
SmoI	CTYRAG	1 cut(s)	35
Sse9I	AATT	6 cut(s)	359, 374, 468, 480, 527, 645
SsiI	CCGC	3 cut(s)	137, 168, 709
SspMI	CTAG	3 cut(s)	255, 275, 545
StyD4I	CCNGG	2 cut(s)	488, 777
TaaI	ACNGT	5 cut(s)	202, 268, 322, 328, 792
TaiI	ACGT	1 cut(s)	296
TaqI	TCGA	1 cut(s)	460
TasI	AATT	6 cut(s)	359, 374, 468, 480, 527, 645
TauI	GCSGC	1 cut(s)	171
TfiI	GAWTC	2 cut(s)	31, 155
Tru1I	TTAA	1 cut(s)	36
Tru9I	TTAA	1 cut(s)	36
TscAI	CASTG	2 cut(s)	327, 426
TseFI	GTSAC	2 cut(s)	322, 596
Tsp45I	GTSAC	2 cut(s)	322, 596
TspDTI	ATGAA	2 cut(s)	63, 449
TspGWI	ACGGA	1 cut(s)	647
TspRI	CASTG	2 cut(s)	327, 426
Vha464I	CTTAAG	1 cut(s)	35
VpaK11BI	GGWCC	1 cut(s)	198
XagI	CCTNNNNNAGG	1 cut(s)	664
XapI	RAATTY	2 cut(s)	359, 468
XbaI	TCTAGA	1 cut(s)	544
XceI	RCATGY	1 cut(s)	696
XmiI	GTMKAC	1 cut(s)	460
XspI	CTAG	3 cut(s)	255, 275, 545

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.