AT5G06430

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015218
Physical Location & Seq
Reverse (-)
1963072 .. 1964467
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06430.1

Sequence Viewer

Length: 585 bp
ATGGAATCAAAGGTTACTACTACGAGCTCTTCTACTTCAAAAGATCCATTCTTTTGCTTGAAATGGCCATGGGATTCAAACAAACAACCCAAATCCTCTTCAAGCGTCTGCGACTTTCAAGGACCGTGGCTCTTCAGATCTATGCAAACTATTGGCTCCATTGCTCTCAGCTCTTTGACTTCATTTGGCCAAAACCCCAATTTCAGACCAAAGAAGAAGCCTTTAAGCAGTAGTGAGCAAGGAGAAGCAGAGCAGAGGGCTTTTGCAGCTGCTTTAGCTAGCCAGAAAGAAGCTACGGTTCTTGAATTTTACTCACACAAATGCAGACTCTGCAATTCTTTGCTCAAATTTGTTTTAGAGGTAGAGAAAAGGAATTCAAATTGGCTCAGCATTACAATGGCAGACGCAGAGAATGAGAAATGGTTTCCTGAGCTTCTCCACTATGACGTAAAGTATGTTCCTTGCTTCGTTTTGTTGGACAAAAATGGACAAGCTTTGGCCAAGACAGGAGTTCCAAGTAGTCGTGCTCATGTCATCGCCGGTATCTCTCATCTTCTCAAGATGAAGCGACCACCATCTAAGTAG

Protein Analysis

194

Amino Acids

21.75

Weight (kDa)

9.4

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 84 - 178 2.9e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06430
fragaria_vesca FvH4_2g40760 FvH4_2g40760
malus_domestica MD15G1036100.v1.1
prunus_persica Prupe.1G390300_v2.0.a1 Prupe.1G390300_v2.0.a1
pyrus_communis pycom15g03360
rosa_chinensis RchiOBHm_Chr6g0299831
rosa_laevigata RLG00000011385
rosa_roxburghii Rroxscaffold_7G00168340
rosa_rugosa Rorug06G0292600
rosa_samantha Rh6AG403700 Rh6BG412000 Rh6CG418100 Rh6DG404900
rosa_wichuraiana Rw6G035320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 38
AcoI YGGCCR 3 cut(s) 65, 187, 498
AcsI RAATTY 3 cut(s) 305, 347, 373
AcuI CTGAAG 1 cut(s) 118
AgsI TTSAA 7 cut(s) 39, 61, 78, 102, 119, 305, 378
AluBI AGCT 7 cut(s) 27, 171, 269, 278, 293, 433, 494
AluI AGCT 7 cut(s) 27, 171, 269, 278, 293, 433, 494
Alw21I GWGCWC 2 cut(s) 29, 529
AlwI GGATC 1 cut(s) 38
AlwNI CAGNNNCTG 1 cut(s) 330
AoxI GGCC 3 cut(s) 65, 187, 498
ApeKI GCWGC 2 cut(s) 266, 269
ApoI RAATTY 3 cut(s) 305, 347, 373
AspS9I GGNCC 1 cut(s) 122
AsuNHI GCTAGC 1 cut(s) 278
AvaII GGWCC 1 cut(s) 122
BalI TGGCCA 3 cut(s) 67, 189, 500
BanII GRGCYC 1 cut(s) 29
Bbv12I GWGCWC 2 cut(s) 29, 529
BbvI GCAGC 2 cut(s) 256, 278
BccI CCATC 1 cut(s) 583
BfaI CTAG 1 cut(s) 279
BglII AGATCT 1 cut(s) 137
BisI GCNGC 2 cut(s) 267, 270
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 2 cut(s) 268, 271
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 157
BmtI GCTAGC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 429
Bpu1102I GCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 68, 125
Bse118I RCCGGY 1 cut(s) 539
Bse3DI GCAATG 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 68, 125
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 3 cut(s) 181, 400, 420
BseXI GCAGC 2 cut(s) 256, 278
BshFI GGCC 3 cut(s) 67, 189, 500
BsiHKAI GWGCWC 2 cut(s) 29, 529
BsiSI CCGG 1 cut(s) 540
BsnI GGCC 3 cut(s) 67, 189, 500
Bsp1286I GDGCHC 2 cut(s) 29, 529
Bsp143I GATC 2 cut(s) 43, 137
Bsp1720I GCTNAGC 1 cut(s) 386
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 3 cut(s) 67, 189, 500
BspCNI CTCAG 3 cut(s) 180, 399, 421
BspLI GGNNCC 1 cut(s) 157
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 1 cut(s) 38
BspQI GCTCTTC 2 cut(s) 34, 137
BsrDI GCAATG 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 539
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 2 cut(s) 68, 125
BssMI GATC 2 cut(s) 43, 137
BssT1I CCWWGG 1 cut(s) 68
Bst4CI ACNGT 2 cut(s) 126, 298
Bst6I CTCTTC 3 cut(s) 34, 103, 137
BstAPI GCANNNNNTGC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 4 cut(s) 167, 386, 429, 579
BstDSI CCRYGG 2 cut(s) 68, 125
BstKTI GATC 2 cut(s) 46, 140
BstMBI GATC 2 cut(s) 43, 137
BstMWI GCNNNNNNNGC 3 cut(s) 266, 275, 330
BstV1I GCAGC 2 cut(s) 256, 278
BstX2I RGATCY 2 cut(s) 43, 137
BstYI RGATCY 2 cut(s) 43, 137
BsuRI GGCC 3 cut(s) 67, 189, 500
BtgI CCRYGG 2 cut(s) 68, 125
BtgZI GCGATG 1 cut(s) 520
Cac8I GCNNGC 1 cut(s) 280
CaiI CAGNNNCTG 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 1 cut(s) 122
CseI GACGC 2 cut(s) 94, 413
CspCI CAANNNNNGTGG 2 cut(s) 107, 142
CviAII CATG 2 cut(s) 69, 530
DdeI CTNAG 4 cut(s) 167, 386, 429, 579
DpnI GATC 2 cut(s) 45, 139
DpnII GATC 2 cut(s) 43, 137
EaeI YGGCCR 3 cut(s) 65, 187, 498
Eam1104I CTCTTC 3 cut(s) 34, 103, 137
EarI CTCTTC 3 cut(s) 34, 103, 137
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 68
FaeI CATG 2 cut(s) 72, 533
FaiI YATR 5 cut(s) 70, 143, 444, 456, 531
FatI CATG 2 cut(s) 68, 529
Fnu4HI GCNGC 2 cut(s) 267, 270
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 267, 270
FspBI CTAG 1 cut(s) 279
GluI GCNGC 2 cut(s) 267, 270
HaeIII GGCC 3 cut(s) 67, 189, 500
HapII CCGG 1 cut(s) 540
HgaI GACGC 2 cut(s) 94, 413
Hin1II CATG 2 cut(s) 72, 533
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 3 cut(s) 5, 74, 327
HpaII CCGG 1 cut(s) 540
Hpy188I TCNGA 2 cut(s) 137, 206
Hpy188III TCNNGA 3 cut(s) 302, 428, 559
HpyCH4III ACNGT 2 cut(s) 126, 298
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 4 cut(s) 145, 266, 324, 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 275, 330
HpyF3I CTNAG 4 cut(s) 167, 386, 429, 579
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 2 cut(s) 72, 533
Kzo9I GATC 2 cut(s) 43, 137
LguI GCTCTTC 2 cut(s) 34, 137
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 4 cut(s) 296, 441, 492, 553
Lsp1109I GCAGC 2 cut(s) 256, 278
MaeI CTAG 1 cut(s) 279
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 13
MalI GATC 2 cut(s) 45, 139
MboI GATC 2 cut(s) 43, 137
MboII GAAGA 5 cut(s) 21, 90, 124, 226, 545
MflI RGATCY 2 cut(s) 43, 137
MhlI GDGCHC 2 cut(s) 29, 529
MlsI TGGCCA 3 cut(s) 67, 189, 500
MluCI AATT 6 cut(s) 199, 305, 334, 347, 373, 379
MluNI TGGCCA 3 cut(s) 67, 189, 500
MlyI GAGTC 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 3 cut(s) 106, 249, 352
Mox20I TGGCCA 3 cut(s) 67, 189, 500
MscI TGGCCA 3 cut(s) 67, 189, 500
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 2 cut(s) 319, 395
Msp20I TGGCCA 3 cut(s) 67, 189, 500
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 540
MwoI GCNNNNNNNGC 3 cut(s) 266, 275, 330
NcoI CCATGG 1 cut(s) 68
NdeII GATC 2 cut(s) 43, 137
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 2 cut(s) 72, 533
NlaIV GGNNCC 1 cut(s) 157
PciSI GCTCTTC 2 cut(s) 34, 137
PfeI GAWTC 2 cut(s) 5, 74
PkrI GCNGC 2 cut(s) 268, 271
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 122
PsrI GAACNNNNNNTAC 2 cut(s) 441, 473
PstNI CAGNNNCTG 1 cut(s) 330
PsuI RGATCY 2 cut(s) 43, 137
PvuII CAGCTG 1 cut(s) 269
RseI CAYNNNNRTG 2 cut(s) 319, 395
SacI GAGCTC 1 cut(s) 29
SapI GCTCTTC 2 cut(s) 34, 137
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 2 cut(s) 267, 270
Sau3AI GATC 2 cut(s) 43, 137
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 321
SduI GDGCHC 2 cut(s) 29, 529
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 2 cut(s) 319, 395
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 6 cut(s) 199, 305, 334, 347, 373, 379
SspMI CTAG 1 cut(s) 279
SstI GAGCTC 1 cut(s) 29
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 126, 298
TaiI ACGT 1 cut(s) 450
TasI AATT 6 cut(s) 199, 305, 334, 347, 373, 379
TfiI GAWTC 2 cut(s) 5, 74
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 2 cut(s) 266, 269
TspDTI ATGAA 2 cut(s) 171, 578
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 3 cut(s) 305, 347, 373
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.