RchiOBHm_Chr6g0299831

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015218
Physical Location & Seq
Forward (+)
60840714 .. 60842720
2007 bp
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UTR
Exon/CDS
Intron
PRQ26922

Sequence Viewer

Length: 609 bp
ATGAGCTCTATACCCAAACAGCCTTTGTTCTGCCTAAAATGGCCATGGGACATGAACCAGACTCCTCAGACTAATACTCCTAGTGTTTGCTCCTTTGAGGGCCCTTGGATATTCAAATCTATGCAAAGTCTTGGGTCTGTTGCGTTCAATTTTGTCCAGTCAGTTTCCAAGTCACGGTCTTCGTTAGTCAATCATTTTAAGCCCTTGCAGTTTGATGCAAGAACCAACCAAAGTAAGAGTCTGAGTCCTGAAGAGCAGGCGGAGGCAGAGCATAGAGCATTCGCCTCCGCATTGGCAAGCAATAAAGAGGCTACAATGCTTGAGTTTTACTCGCCCAAATGCAGGCTGTGCAGTTCTTTACTTAATTCTGTGGTGGAGGTTGAGCGTAGGAACTCAGACTGGCTTAACATTGTTATGGCAGATGCAGAGAATGACAAATGGCTACCCGAGCTCCTTCATTACGACATTAGATATGTACCTTGCTTTGTATTACTGGACAAGAAGGGGAGGGCACTGGCAAAGACTGGTATTCCAAATAGTCGACTACATGTATTAGCAGGTCTCTCTCATCTTCTGAAGATGAAGTGTCCTAGCAAAAGAACAACCTAA

Protein Analysis

202

Amino Acids

22.88

Weight (kDa)

9.18

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 89 - 171 1.2e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015218)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06430
fragaria_vesca FvH4_2g40760 FvH4_2g40760
malus_domestica MD15G1036100.v1.1
prunus_persica Prupe.1G390300_v2.0.a1 Prupe.1G390300_v2.0.a1
pyrus_communis pycom15g03360
rosa_chinensis RchiOBHm_Chr6g0299831
rosa_laevigata RLG00000011385
rosa_roxburghii Rroxscaffold_7G00168340
rosa_rugosa Rorug06G0292600
rosa_samantha Rh6AG403700 Rh6BG412000 Rh6CG418100 Rh6DG404900
rosa_wichuraiana Rw6G035320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 548
AccI GTMKAC 1 cut(s) 541
AciI CCGC 2 cut(s) 260, 288
AcoI YGGCCR 1 cut(s) 41
AcuI CTGAAG 2 cut(s) 270, 596
AfaI GTAC 1 cut(s) 477
AfiI CCNNNNNNNGG 2 cut(s) 174, 342
AflIII ACRYGT 1 cut(s) 547
AgsI TTSAA 2 cut(s) 115, 148
AluBI AGCT 2 cut(s) 6, 451
AluI AGCT 2 cut(s) 6, 451
Alw21I GWGCWC 2 cut(s) 8, 453
Alw26I GTCTC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 2 cut(s) 41, 100
ApaI GGGCCC 1 cut(s) 104
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AspS9I GGNCC 2 cut(s) 100, 101
AvaI CYCGRG 1 cut(s) 446
BaeGI GKGCMC 2 cut(s) 104, 514
BalI TGGCCA 1 cut(s) 43
BanII GRGCYC 3 cut(s) 8, 104, 453
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 8, 453
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 2 cut(s) 81, 591
BfuAI ACCTGC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 100, 101
BmiI GGNNCC 1 cut(s) 102
BmsI GCATC 2 cut(s) 205, 412
BpiI GAAGAC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BpuEI CTTGAG 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 566
BsaJI CCNNGG 2 cut(s) 44, 104
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 342
Bse1I ACTGG 5 cut(s) 157, 404, 498, 519, 529
BseDI CCNNGG 2 cut(s) 44, 104
BseLI CCNNNNNNNGG 2 cut(s) 174, 342
BseMII CTCAG 3 cut(s) 80, 233, 408
BseNI ACTGG 5 cut(s) 157, 404, 498, 519, 529
BseRI GAGGAG 1 cut(s) 54
BseSI GKGCMC 2 cut(s) 104, 514
BsgI GTGCAG 1 cut(s) 370
BshFI GGCC 2 cut(s) 43, 102
BsiHKAI GWGCWC 2 cut(s) 8, 453
BsiHKCI CYCGRG 1 cut(s) 446
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 174, 342
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 2 cut(s) 43, 102
Bso31I GGTCTC 1 cut(s) 566
BsoBI CYCGRG 1 cut(s) 446
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 4 cut(s) 8, 104, 453, 514
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 2 cut(s) 260, 288
BspANI GGCC 2 cut(s) 43, 102
BspCNI CTCAG 3 cut(s) 79, 234, 407
BspLI GGNNCC 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 548
BspQI GCTCTTC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 566
BsrI ACTGG 5 cut(s) 157, 404, 498, 519, 529
BssECI CCNNGG 2 cut(s) 44, 104
BssT1I CCWWGG 2 cut(s) 44, 104
Bst4CI ACNGT 1 cut(s) 177
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 348
BstC8I GCNNGC 3 cut(s) 258, 298, 344
BstDEI CTNAG 3 cut(s) 66, 242, 394
BstDSI CCRYGG 1 cut(s) 44
BstMAI GTCTC 1 cut(s) 566
BstMWI GCNNNNNNNGC 2 cut(s) 348, 448
BstNSI RCATGY 1 cut(s) 551
BstSLI GKGCMC 2 cut(s) 104, 514
BstV2I GAAGAC 1 cut(s) 171
BsuRI GGCC 2 cut(s) 43, 102
BtgI CCRYGG 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 512
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 3 cut(s) 258, 298, 344
Cfr13I GGNCC 2 cut(s) 100, 101
Csp6I GTAC 1 cut(s) 476
CviAII CATG 3 cut(s) 45, 52, 548
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 3 cut(s) 66, 242, 394
EaeI YGGCCR 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 2 cut(s) 6, 451
Eco130I CCWWGG 2 cut(s) 44, 104
Eco24I GRGCYC 3 cut(s) 8, 104, 453
Eco31I GGTCTC 1 cut(s) 566
Eco53kI GAGCTC 2 cut(s) 6, 451
Eco57I CTGAAG 2 cut(s) 270, 596
Eco88I CYCGRG 1 cut(s) 446
EcoICRI GAGCTC 2 cut(s) 6, 451
EcoO109I RGGNCCY 2 cut(s) 100, 101
EcoT14I CCWWGG 2 cut(s) 44, 104
EcoT38I GRGCYC 3 cut(s) 8, 104, 453
ErhI CCWWGG 2 cut(s) 44, 104
FaeI CATG 3 cut(s) 48, 55, 551
FaiI YATR 8 cut(s) 11, 46, 53, 122, 273, 416, 474, 549
FaqI GGGAC 1 cut(s) 62
FatI CATG 3 cut(s) 44, 51, 547
FblI GTMKAC 1 cut(s) 541
FriOI GRGCYC 3 cut(s) 8, 104, 453
FspBI CTAG 2 cut(s) 81, 591
HaeIII GGCC 2 cut(s) 43, 102
Hin1II CATG 3 cut(s) 48, 55, 551
HincII GTYRAC 1 cut(s) 542
HindII GTYRAC 1 cut(s) 542
HinfI GANTC 3 cut(s) 61, 238, 244
Hpy166II GTNNAC 1 cut(s) 542
Hpy188I TCNGA 4 cut(s) 69, 243, 397, 576
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 1 cut(s) 542
HpyAV CCTTC 2 cut(s) 464, 496
HpyCH4III ACNGT 1 cut(s) 177
HpyCH4V TGCA 6 cut(s) 124, 208, 218, 342, 351, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 348, 448
HpyF3I CTNAG 3 cut(s) 66, 242, 394
Hsp92II CATG 3 cut(s) 48, 55, 551
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 2 cut(s) 95, 456
LweI GCATC 2 cut(s) 205, 412
MaeI CTAG 2 cut(s) 81, 591
MaeIII GTNAC 1 cut(s) 171
MboII GAAGA 4 cut(s) 171, 263, 563, 589
MhlI GDGCHC 4 cut(s) 8, 104, 453, 514
MlsI TGGCCA 1 cut(s) 43
MluCI AATT 2 cut(s) 148, 364
MluNI TGGCCA 1 cut(s) 43
MlyI GAGTC 3 cut(s) 55, 247, 253
MnlI CCTC 7 cut(s) 75, 91, 256, 295, 301, 370, 501
Mox20I TGGCCA 1 cut(s) 43
MscI TGGCCA 1 cut(s) 43
MseI TTAA 3 cut(s) 198, 363, 405
MslI CAYNNNNRTG 1 cut(s) 413
Msp20I TGGCCA 1 cut(s) 43
Mva1269I GAATGC 1 cut(s) 278
MwoI GCNNNNNNNGC 2 cut(s) 348, 448
NcoI CCATGG 1 cut(s) 44
NlaIII CATG 3 cut(s) 48, 55, 551
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 171
NspI RCATGY 1 cut(s) 551
PciI ACATGT 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 246
PctI GAATGC 1 cut(s) 278
PleI GAGTC 3 cut(s) 55, 246, 252
PpsI GAGTC 3 cut(s) 55, 246, 252
PscI ACATGT 1 cut(s) 547
Psp124BI GAGCTC 2 cut(s) 8, 453
PspN4I GGNNCC 1 cut(s) 102
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 2 cut(s) 100, 101
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 2 cut(s) 8, 453
SalI GTCGAC 1 cut(s) 540
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 3 cut(s) 198, 363, 405
Sau96I GGNCC 2 cut(s) 100, 101
SchI GAGTC 3 cut(s) 55, 247, 253
SduI GDGCHC 4 cut(s) 8, 104, 453, 514
SetI ASST 6 cut(s) 8, 381, 453, 481, 562, 608
SfaNI GCATC 2 cut(s) 205, 412
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 2 cut(s) 148, 364
SsiI CCGC 2 cut(s) 260, 288
SspMI CTAG 2 cut(s) 81, 591
SstI GAGCTC 2 cut(s) 8, 453
StyI CCWWGG 2 cut(s) 44, 104
TaaI ACNGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 541
TasI AATT 2 cut(s) 148, 364
Tru1I TTAA 3 cut(s) 198, 363, 405
Tru9I TTAA 3 cut(s) 198, 363, 405
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 1 cut(s) 171
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 3 cut(s) 68, 446, 596
TspRI CASTG 1 cut(s) 519
XceI RCATGY 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 541
XspI CTAG 2 cut(s) 81, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.