Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009184

Physical Location & Seq

Genomic Coordinates Forward (+)

2667361 .. 2669923

Gene Structure 2563 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G08300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1044 bp

ATGTCTAGACAAGTGGCAAGGCTTATCGGATCTCTGTCATCAAAAGCTCGCCGTTGTTCTACCGGCGGCTCAGAGGTTTTTCCGTCATGCCAGTCTCTCACTTCGTTGACCCAATCTCGCTCCTTCGCGTCGGATCCTCATCCTCCTGCGGCGGTTTTCGTTGACAAAAACACACGAGTCCTCTGTCAAGGCATCACCGGAAAGAACGGCACTTTCCACACTGAACAAGCCATTGAGTACGGCACCAAAATGGTGGCAGGAGTGACACCGAAGAAGGGTGGAACAGAACACTTGGGTCTCCCAGTTTTCAACTCTGTTGCTGAAGCTAAGGCTGACACCAAAGCCAATGCCTCTGTGATTTATGTTCCTGCTCCTTTTGCTGCTGCTGCTATTATGGAGGGTATTGAGGCTGAGTTAGATCTTATTGTGTGTATCACCGAAGGAATCCCTCAACATGACATGGTACGTGTAAAGCATGCTCTTAACAGTCAATCGAAAACTAGGCTGATTGGTCCAAACTGCCCTGGGATTATCAAGCCTGGTGAATGCAAAATTGGAATTATGCCGGGATACATCCACAAGCCTGGGAAGATTGGAATTGTCTCACGATCTGGAACCTTGACATATGAAGCTGTTTTCCAAACAACTGCAGTGGGTCTAGGCCAATCTACTTGTGTAGGAATCGGTGGGGATCCTTTTAACGGGACAAACTTTGTCGACTGCTTGGAGAAATTCTTTGTTGATCCTCAAACAGAAGGTATCGTTCTCATTGGAGAAATCGGAGGCACTGCAGAAGAAGATGCTGCAGCATTGATAAAGGCAAGTGGTACTGAGAAGCCTGTTGTTGCTTTTATTGCTGGACTTACTGCACCACCGGGTCGTCGAATGGGTCATGCCGGAGCCATTGTTTCCGGTGGTAAAGGTACGGCTCAGGACAAAATTAAGAGTTTGAATGATGCTGGAGTGAAGGTTGTTGAATCTCCGGCTAAGATTGGATCAGCGATGTATGAGCTCTTTCAAGAAAGAGGTCTTTTGAAGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004774 GO:0004775 GO:0004776 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006104 GO:0006105 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019637 GO:0019693 GO:0019752 GO:0030312 GO:0031974 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046483 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0071944 GO:0072350 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00640 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

CoA_binding Ligase_CoA

Protein Analysis

347

Amino Acids

36.15

Weight (kDa)

8.55

Isoelectric Point (pI)

37.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CoA_binding	PF02629	55 - 148	2.4e-32	CoA binding domain
Succ_CoA_lig	PF13607	195 - 303	3.8e-10	Succinyl-CoA ligase like flavodoxin domain
Ligase_CoA	PF00549	201 - 321	7.4e-29	CoA-ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08300 AT5G23250 AT5G23250 AT5G23250
fragaria_vesca	FvH4_5g18391
malus_domestica	MD06G1239800.v1.1 MD14G1245700.v1.1
prunus_persica	Prupe.5G243700_v2.0.a1 Prupe.5G243700_v2.0.a1
pyrus_communis	pycom06g21530 pycom14g20660
rosa_chinensis	RchiOBHm_Chr7g0177081
rosa_laevigata	RLG00000005558
rosa_multiflora	Rmu_sc0005310.1_g000024 Rmu_sc0005310.1_g000025 Rmu_sc0005912.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276110
rosa_rugosa	Rorug06G0403600
rosa_samantha	Rh7AG003000 Rh7CG002600 Rh7DG003100
rosa_wichuraiana	Rw7G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	242
AccI	GTMKAC	1 cut(s)	717
AccII	CGCG	1 cut(s)	128
AciI	CCGC	3 cut(s)	66, 149, 152
AclWI	GGATC	7 cut(s)	37, 128, 141, 686, 699, 737, 1003
AcsI	RAATTY	1 cut(s)	731
AcuI	CTGAAG	1 cut(s)	342
AfaI	GTAC	4 cut(s)	239, 465, 829, 925
AfiI	CCNNNNNNNGG	2 cut(s)	275, 701
AflIII	ACRYGT	1 cut(s)	466
AgsI	TTSAA	5 cut(s)	310, 952, 977, 1019, 1036
AjnI	CCWGG	3 cut(s)	523, 538, 583
AjuI	GAANNNNNNNTTGG	2 cut(s)	537, 569
AluBI	AGCT	4 cut(s)	47, 326, 632, 1012
AluI	AGCT	4 cut(s)	47, 326, 632, 1012
Alw21I	GWGCWC	1 cut(s)	1014
Alw26I	GTCTC	3 cut(s)	99, 302, 607
AlwI	GGATC	7 cut(s)	37, 128, 141, 686, 699, 737, 1003
AoxI	GGCC	1 cut(s)	661
ApeKI	GCWGC	5 cut(s)	380, 383, 386, 803, 806
ApoI	RAATTY	1 cut(s)	731
AspS9I	GGNCC	1 cut(s)	512
AsuC2I	CCSGG	2 cut(s)	567, 876
AsuHPI	GGTGA	3 cut(s)	187, 427, 554
AvaII	GGWCC	1 cut(s)	512
BamHI	GGATCC	2 cut(s)	133, 691
BanI	GGYRCC	1 cut(s)	242
BanII	GRGCYC	1 cut(s)	1014
BauI	CACGAG	1 cut(s)	174
Bbv12I	GWGCWC	1 cut(s)	1014
BbvI	GCAGC	5 cut(s)	367, 370, 373, 790, 818
BceAI	ACGGC	4 cut(s)	36, 223, 256, 942
BciT130I	CCWGG	3 cut(s)	525, 540, 585
BciVI	GTATCC	1 cut(s)	563
BcnI	CCSGG	2 cut(s)	567, 876
BcoDI	GTCTC	3 cut(s)	99, 302, 607
BfaI	CTAG	3 cut(s)	6, 501, 659
BfmI	CTRYAG	3 cut(s)	648, 789, 804
BfuI	GTATCC	1 cut(s)	563
BglII	AGATCT	1 cut(s)	418
BisI	GCNGC	7 cut(s)	67, 150, 381, 384, 387, 804, 807
BlsI	GCNGC	7 cut(s)	68, 151, 382, 385, 388, 805, 808
Bme1390I	CCNGG	5 cut(s)	525, 540, 567, 585, 876
Bme18I	GGWCC	1 cut(s)	512
BmgT120I	GGNCC	1 cut(s)	512
BmiI	GGNNCC	5 cut(s)	135, 244, 616, 693, 901
BmrFI	CCNGG	5 cut(s)	525, 540, 567, 585, 876
BmrI	ACTGGG	1 cut(s)	296
BmsI	GCATC	3 cut(s)	201, 790, 946
BmuI	ACTGGG	1 cut(s)	296
BpmI	CTGGAG	1 cut(s)	981
Bpu10I	CCTNAGC	2 cut(s)	327, 930
BpuMI	CCSGG	2 cut(s)	567, 876
BsaAI	YACGTR	1 cut(s)	467
BsaBI	GATNNNNATC	1 cut(s)	138
BsaI	GGTCTC	1 cut(s)	302
BsaJI	CCNNGG	3 cut(s)	523, 524, 584
BsaWI	WCCGGW	2 cut(s)	197, 911
BsaXI	ACNNNNNCTCC	4 cut(s)	891, 921, 954, 984
Bsc4I	CCNNNNNNNGG	2 cut(s)	275, 701
Bse118I	RCCGGY	1 cut(s)	62
Bse1I	ACTGG	2 cut(s)	91, 302
Bse8I	GATNNNNATC	1 cut(s)	138
BseBI	CCWGG	3 cut(s)	525, 540, 585
BseDI	CCNNGG	3 cut(s)	523, 524, 584
BseGI	GGATG	2 cut(s)	139, 573
BseJI	GATNNNNATC	1 cut(s)	138
BseLI	CCNNNNNNNGG	2 cut(s)	275, 701
BseMII	CTCAG	4 cut(s)	84, 402, 822, 944
BseNI	ACTGG	2 cut(s)	91, 302
BseXI	GCAGC	5 cut(s)	367, 370, 373, 790, 818
BsgI	GTGCAG	1 cut(s)	852
Bsh1236I	CGCG	1 cut(s)	128
BshFI	GGCC	1 cut(s)	663
BshNI	GGYRCC	1 cut(s)	242
BsiHKAI	GWGCWC	1 cut(s)	1014
BsiSI	CCGG	7 cut(s)	63, 198, 566, 875, 897, 912, 983
BslFI	GGGAC	1 cut(s)	718
BslI	CCNNNNNNNGG	2 cut(s)	275, 701
BsmAI	GTCTC	3 cut(s)	99, 302, 607
BsmFI	GGGAC	1 cut(s)	718
BsmI	GAATGC	1 cut(s)	551
BsnI	GGCC	1 cut(s)	663
Bso31I	GGTCTC	1 cut(s)	302
Bsp1286I	GDGCHC	1 cut(s)	1014
Bsp143I	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
BspACI	CCGC	3 cut(s)	66, 149, 152
BspANI	GGCC	1 cut(s)	663
BspCNI	CTCAG	4 cut(s)	83, 403, 823, 943
BspFNI	CGCG	1 cut(s)	128
BspLI	GGNNCC	5 cut(s)	135, 244, 616, 693, 901
BspMAI	CTGCAG	3 cut(s)	652, 793, 808
BspPI	GGATC	7 cut(s)	37, 128, 141, 686, 699, 737, 1003
BspT107I	GGYRCC	1 cut(s)	242
BspTNI	GGTCTC	1 cut(s)	302
BsrFI	RCCGGY	1 cut(s)	62
BsrI	ACTGG	2 cut(s)	91, 302
BssAI	RCCGGY	1 cut(s)	62
BssECI	CCNNGG	3 cut(s)	523, 524, 584
BssMI	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
BssSI	CACGAG	1 cut(s)	174
Bst2BI	CACGAG	1 cut(s)	174
Bst2UI	CCWGG	3 cut(s)	525, 540, 585
Bst4CI	ACNGT	1 cut(s)	488
BstBAI	YACGTR	1 cut(s)	467
BstC8I	GCNNGC	2 cut(s)	49, 477
BstDEI	CTNAG	6 cut(s)	70, 327, 411, 831, 930, 987
BstF5I	GGATG	2 cut(s)	139, 573
BstFNI	CGCG	1 cut(s)	128
BstKTI	GATC	7 cut(s)	32, 136, 421, 611, 694, 745, 998
BstMAI	GTCTC	3 cut(s)	99, 302, 607
BstMBI	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
BstMWI	GCNNNNNNNGC	3 cut(s)	377, 386, 854
BstNI	CCWGG	3 cut(s)	525, 540, 585
BstNSI	RCATGY	1 cut(s)	479
BstSCI	CCNGG	5 cut(s)	523, 538, 565, 583, 874
BstSFI	CTRYAG	3 cut(s)	648, 789, 804
BstUI	CGCG	1 cut(s)	128
BstV1I	GCAGC	5 cut(s)	367, 370, 373, 790, 818
BstX2I	RGATCY	4 cut(s)	29, 133, 418, 691
BstXI	CCANNNNNNTGG	2 cut(s)	253, 584
BstYI	RGATCY	4 cut(s)	29, 133, 418, 691
BsuI	GTATCC	1 cut(s)	563
BsuRI	GGCC	1 cut(s)	663
BtgZI	GCGATG	1 cut(s)	1016
BtsCI	GGATG	2 cut(s)	139, 573
BtsI	GCAGTG	2 cut(s)	657, 786
BtsIMutI	CAGTG	3 cut(s)	219, 657, 786
Cac8I	GCNNGC	2 cut(s)	49, 477
Cfr10I	RCCGGY	1 cut(s)	62
Cfr13I	GGNCC	1 cut(s)	512
CseI	GACGC	1 cut(s)	117
Csp6I	GTAC	4 cut(s)	238, 464, 828, 924
CspCI	CAANNNNNGTGG	2 cut(s)	633, 668
CviAII	CATG	5 cut(s)	87, 455, 460, 476, 893
CviQI	GTAC	4 cut(s)	238, 464, 828, 924
DdeI	CTNAG	6 cut(s)	70, 327, 411, 831, 930, 987
DpnI	GATC	7 cut(s)	31, 135, 420, 610, 693, 744, 997
DpnII	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
Ecl136II	GAGCTC	1 cut(s)	1012
Eco24I	GRGCYC	1 cut(s)	1014
Eco31I	GGTCTC	1 cut(s)	302
Eco47I	GGWCC	1 cut(s)	512
Eco53kI	GAGCTC	1 cut(s)	1012
Eco57I	CTGAAG	1 cut(s)	342
EcoICRI	GAGCTC	1 cut(s)	1012
EcoRII	CCWGG	3 cut(s)	523, 538, 583
EcoT38I	GRGCYC	1 cut(s)	1014
FaeI	CATG	5 cut(s)	90, 458, 463, 479, 896
FaqI	GGGAC	1 cut(s)	718
FatI	CATG	5 cut(s)	86, 454, 459, 475, 892
FauNDI	CATATG	1 cut(s)	625
FblI	GTMKAC	1 cut(s)	717
Fnu4HI	GCNGC	7 cut(s)	67, 150, 381, 384, 387, 804, 807
FokI	GGATG	2 cut(s)	126, 560
FriOI	GRGCYC	1 cut(s)	1014
Fsp4HI	GCNGC	7 cut(s)	67, 150, 381, 384, 387, 804, 807
FspBI	CTAG	3 cut(s)	6, 501, 659
GluI	GCNGC	7 cut(s)	67, 150, 381, 384, 387, 804, 807
GsuI	CTGGAG	1 cut(s)	981
HaeIII	GGCC	1 cut(s)	663
HapII	CCGG	7 cut(s)	63, 198, 566, 875, 897, 912, 983
HgaI	GACGC	1 cut(s)	117
Hin1II	CATG	5 cut(s)	90, 458, 463, 479, 896
HincII	GTYRAC	3 cut(s)	108, 163, 718
HindII	GTYRAC	3 cut(s)	108, 163, 718
HinfI	GANTC	4 cut(s)	177, 444, 681, 977
HpaII	CCGG	7 cut(s)	63, 198, 566, 875, 897, 912, 983
HphI	GGTGA	3 cut(s)	187, 427, 554
Hpy166II	GTNNAC	3 cut(s)	108, 163, 718
Hpy188I	TCNGA	4 cut(s)	29, 73, 133, 782
Hpy188III	TCNNGA	5 cut(s)	6, 606, 612, 932, 1019
Hpy8I	GTNNAC	3 cut(s)	108, 163, 718
Hpy99I	CGWCG	2 cut(s)	133, 885
HpyAV	CCTTC	5 cut(s)	133, 268, 434, 749, 961
HpyCH4III	ACNGT	1 cut(s)	488
HpyCH4IV	ACGT	1 cut(s)	466
HpyCH4V	TGCA	5 cut(s)	549, 650, 791, 806, 869
HpyF10VI	GCNNNNNNNGC	3 cut(s)	377, 386, 854
HpyF3I	CTNAG	6 cut(s)	70, 327, 411, 831, 930, 987
HpySE526I	ACGT	1 cut(s)	466
Hsp92II	CATG	5 cut(s)	90, 458, 463, 479, 896
Kzo9I	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
LmnI	GCTCC	3 cut(s)	125, 376, 899
Lsp1109I	GCAGC	5 cut(s)	367, 370, 373, 790, 818
LweI	GCATC	3 cut(s)	201, 790, 946
MaeI	CTAG	3 cut(s)	6, 501, 659
MaeII	ACGT	1 cut(s)	466
MaeIII	GTNAC	1 cut(s)	262
MalI	GATC	7 cut(s)	31, 135, 420, 610, 693, 744, 997
MboI	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
MboII	GAAGA	4 cut(s)	283, 601, 806, 809
MflI	RGATCY	4 cut(s)	29, 133, 418, 691
MhlI	GDGCHC	1 cut(s)	1014
MluCI	AATT	5 cut(s)	552, 558, 597, 731, 939
MlyI	GAGTC	1 cut(s)	186
MmeI	TCCRAC	1 cut(s)	111
MseI	TTAA	3 cut(s)	483, 699, 942
MslI	CAYNNNNRTG	1 cut(s)	248
MspI	CCGG	7 cut(s)	63, 198, 566, 875, 897, 912, 983
MspR9I	CCNGG	5 cut(s)	525, 540, 567, 585, 876
Mva1269I	GAATGC	1 cut(s)	551
MvaI	CCWGG	3 cut(s)	525, 540, 585
MvnI	CGCG	1 cut(s)	128
MwoI	GCNNNNNNNGC	3 cut(s)	377, 386, 854
NciI	CCSGG	2 cut(s)	567, 876
NdeI	CATATG	1 cut(s)	625
NdeII	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
NlaIII	CATG	5 cut(s)	90, 458, 463, 479, 896
NlaIV	GGNNCC	5 cut(s)	135, 244, 616, 693, 901
NmuCI	GTSAC	1 cut(s)	262
NspI	RCATGY	1 cut(s)	479
PaeI	GCATGC	1 cut(s)	479
PasI	CCCWGGG	1 cut(s)	524
PctI	GAATGC	1 cut(s)	551
PfeI	GAWTC	3 cut(s)	444, 681, 977
PkrI	GCNGC	7 cut(s)	68, 151, 382, 385, 388, 805, 808
PleI	GAGTC	1 cut(s)	185
PpsI	GAGTC	1 cut(s)	185
Ppu21I	YACGTR	1 cut(s)	467
Psp124BI	GAGCTC	1 cut(s)	1014
Psp6I	CCWGG	3 cut(s)	523, 538, 583
PspGI	CCWGG	3 cut(s)	523, 538, 583
PspN4I	GGNNCC	5 cut(s)	135, 244, 616, 693, 901
PspPI	GGNCC	1 cut(s)	512
PstI	CTGCAG	3 cut(s)	652, 793, 808
PsuI	RGATCY	4 cut(s)	29, 133, 418, 691
RsaI	GTAC	4 cut(s)	239, 465, 829, 925
RsaNI	GTAC	4 cut(s)	238, 464, 828, 924
RseI	CAYNNNNRTG	1 cut(s)	248
SacI	GAGCTC	1 cut(s)	1014
SalI	GTCGAC	1 cut(s)	716
SaqAI	TTAA	3 cut(s)	483, 699, 942
SatI	GCNGC	7 cut(s)	67, 150, 381, 384, 387, 804, 807
Sau3AI	GATC	7 cut(s)	29, 133, 418, 608, 691, 742, 995
Sau96I	GGNCC	1 cut(s)	512
SchI	GAGTC	1 cut(s)	186
ScrFI	CCNGG	5 cut(s)	525, 540, 567, 585, 876
SduI	GDGCHC	1 cut(s)	1014
SfaNI	GCATC	3 cut(s)	201, 790, 946
SfcI	CTRYAG	3 cut(s)	648, 789, 804
SinI	GGWCC	1 cut(s)	512
SmiMI	CAYNNNNRTG	1 cut(s)	248
SphI	GCATGC	1 cut(s)	479
Sse9I	AATT	5 cut(s)	552, 558, 597, 731, 939
SsiI	CCGC	3 cut(s)	66, 149, 152
SspMI	CTAG	3 cut(s)	6, 501, 659
SstI	GAGCTC	1 cut(s)	1014
StyD4I	CCNGG	5 cut(s)	523, 538, 565, 583, 874
TaaI	ACNGT	1 cut(s)	488
TaiI	ACGT	1 cut(s)	469
TaqI	TCGA	3 cut(s)	494, 717, 883
TasI	AATT	5 cut(s)	552, 558, 597, 731, 939
TauI	GCSGC	2 cut(s)	69, 152
TfiI	GAWTC	3 cut(s)	444, 681, 977
Tru1I	TTAA	3 cut(s)	483, 699, 942
Tru9I	TTAA	3 cut(s)	483, 699, 942
TscAI	CASTG	3 cut(s)	226, 657, 793
TseFI	GTSAC	1 cut(s)	262
TseI	GCWGC	5 cut(s)	380, 383, 386, 803, 806
Tsp45I	GTSAC	1 cut(s)	262
TspDTI	ATGAA	1 cut(s)	642
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	3 cut(s)	226, 657, 793
VpaK11BI	GGWCC	1 cut(s)	512
XapI	RAATTY	1 cut(s)	731
XbaI	TCTAGA	1 cut(s)	5
XceI	RCATGY	1 cut(s)	479
XmiI	GTMKAC	1 cut(s)	717
XspI	CTAG	3 cut(s)	6, 501, 659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.