Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009184

Physical Location & Seq

Genomic Coordinates Forward (+)

7830318 .. 7832843

Gene Structure 2526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G23250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGTCTAGGCAGGTCACGAGACTTCTCGGATCTCTTCGCCATTCCGGCGGCGGCTGCAGCGGCAGCTCAAAGGTCTGCTCTCTCACTTCATTGGTACAGTCTCGCTCCTTCGGGACAACTCCTCCGCCTCCTGCGGCGGTCTTTGTCGACAAAAATACACGTGTCATCTGCCAAGGTATTACCGGAAAGAACGGGACCTTCCACACTGAACAAGCCATTGAATACGGCACCAAAATGGTAGCAGGAGTGACACCAAAGAAGGGTGGAACAGAACATTTGGGGCTACCTGTTTTCAACACTGTTGCTGAAGCTAAGGCTGAGACTAAAGCCAATGCCTCTGTGATCTACGTACCTGCGCCTTTCGCTGCTGCTGCTATCATGGAAGGTTTAGCTGCTGAGTTAGATCTTATTGTTTGTATCACTGAAGGAATTCCTCAACATGACATGGTTCGTGTAAAGGCTGCTCTTAACAGTCAATCAAAAACTAGGCTGATTGGCCCTAATTGTCCTGGTATTATCAAACCTGGTGAATGCAAGATTGGGATCATGCCAGGATACATACACAAGCCGGGGAAGATAGGGATTGTTTCTCGATCTGGAACATTGACATATGAAGCTGTTTTCCAAACTACTGCAGTAGGTTTGGGGCAATCTACTTGTGTAGGAATTGGTGGGGATCCGTTTAATGGGACAAATTTTGTTGACTGCTTGGAGAAGTTCTTTGTTGACCCCCAAACAGAAGGTATCGTTCTCATTGGAGAAATCGGTGGGACTGCAGAAGAAGATGCCGCTGCTTTGATAAAGGAAAATGGTACAGACAAGCCTGTAGTTGCTTTCATAGCCGGACTTACTGCACCACCAGGGCGTCGTATGGGTCATGCGGGAGCCATTGTTTCCGGTGGTAAGGGCACGGCTCAAGACAAGATAAAGAGCCTAAGAGACGCAGGAGTTAAGGTGGTTGAGTCACCAGCTAAGATTGGTGCGGCGATGTTTGAGCTTTTCCAAGAAAGAGGTCTTTTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004774 GO:0004775 GO:0004776 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006104 GO:0006105 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019637 GO:0019693 GO:0019752 GO:0030312 GO:0031974 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046483 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0071944 GO:0072350 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00640 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

CoA_binding Ligase_CoA

Protein Analysis

341

Amino Acids

35.32

Weight (kDa)

8.84

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CoA_binding	PF02629	50 - 143	4.2e-32	CoA binding domain
Succ_CoA_lig	PF13607	190 - 322	1.2e-09	Succinyl-CoA ligase like flavodoxin domain
Ligase_CoA	PF00549	196 - 317	5.6e-29	CoA-ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08300 AT5G23250 AT5G23250 AT5G23250
fragaria_vesca	FvH4_5g18391
malus_domestica	MD06G1239800.v1.1 MD14G1245700.v1.1
prunus_persica	Prupe.5G243700_v2.0.a1 Prupe.5G243700_v2.0.a1
pyrus_communis	pycom06g21530 pycom14g20660
rosa_chinensis	RchiOBHm_Chr7g0177081
rosa_laevigata	RLG00000005558
rosa_multiflora	Rmu_sc0005310.1_g000024 Rmu_sc0005310.1_g000025 Rmu_sc0005912.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276110
rosa_rugosa	Rorug06G0403600
rosa_samantha	Rh7AG003000 Rh7CG002600 Rh7DG003100
rosa_wichuraiana	Rw7G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	361
AccB1I	GGYRCC	1 cut(s)	227
AccI	GTMKAC	1 cut(s)	147
AciI	CCGC	9 cut(s)	48, 51, 60, 125, 134, 137, 789, 881, 983
AclWI	GGATC	4 cut(s)	37, 551, 671, 684
AcsI	RAATTY	2 cut(s)	429, 694
AcuI	CTGAAG	2 cut(s)	327, 444
AcvI	CACGTG	1 cut(s)	161
AcyI	GRCGYC	1 cut(s)	865
AfaI	GTAC	3 cut(s)	96, 351, 814
AfiI	CCNNNNNNNGG	2 cut(s)	260, 686
AflIII	ACRYGT	2 cut(s)	158, 160
AgsI	TTSAA	3 cut(s)	221, 295, 1021
AjnI	CCWGG	4 cut(s)	508, 523, 550, 859
AjuI	GAANNNNNNNTTGG	2 cut(s)	522, 554
AluBI	AGCT	6 cut(s)	66, 311, 392, 617, 971, 997
AluI	AGCT	6 cut(s)	66, 311, 392, 617, 971, 997
Alw26I	GTCTC	4 cut(s)	13, 105, 314, 933
AlwI	GGATC	4 cut(s)	37, 551, 671, 684
AoxI	GGCC	1 cut(s)	496
ApeKI	GCWGC	9 cut(s)	54, 57, 63, 365, 368, 371, 392, 461, 791
ApoI	RAATTY	2 cut(s)	429, 694
Asp700I	GAANNNNTTC	1 cut(s)	429
AspLEI	GCGC	1 cut(s)	358
AspS9I	GGNCC	2 cut(s)	195, 497
AsuC2I	CCSGG	1 cut(s)	570
AsuHPI	GGTGA	2 cut(s)	539, 957
AvaII	GGWCC	1 cut(s)	195
BaeGI	GKGCMC	1 cut(s)	911
BamHI	GGATCC	1 cut(s)	676
BanI	GGYRCC	1 cut(s)	227
BauI	CACGAG	1 cut(s)	16
BbrPI	CACGTG	1 cut(s)	161
BbvI	GCAGC	9 cut(s)	41, 69, 75, 352, 355, 358, 379, 448, 778
BceAI	ACGGC	2 cut(s)	241, 927
BciT130I	CCWGG	4 cut(s)	510, 525, 552, 861
BciVI	GTATCC	1 cut(s)	548
BcnI	CCSGG	1 cut(s)	570
BcoDI	GTCTC	4 cut(s)	13, 105, 314, 933
BfaI	CTAG	2 cut(s)	6, 486
BfmI	CTRYAG	4 cut(s)	55, 633, 774, 825
BfuAI	ACCTGC	1 cut(s)	361
BfuI	GTATCC	1 cut(s)	548
BglI	GCCNNNNNGGC	1 cut(s)	45
BglII	AGATCT	1 cut(s)	403
Bme1390I	CCNGG	5 cut(s)	510, 525, 552, 570, 861
Bme18I	GGWCC	1 cut(s)	195
BmgT120I	GGNCC	2 cut(s)	195, 497
BmiI	GGNNCC	4 cut(s)	196, 229, 678, 886
BmrFI	CCNGG	5 cut(s)	510, 525, 552, 570, 861
BmsI	GCATC	1 cut(s)	775
Bpu10I	CCTNAGC	1 cut(s)	312
BpuEI	CTTGAG	1 cut(s)	900
BpuMI	CCSGG	1 cut(s)	570
BsaAI	YACGTR	2 cut(s)	161, 349
BsaBI	GATNNNNATC	1 cut(s)	542
BsaHI	GRCGYC	1 cut(s)	865
BsaJI	CCNNGG	3 cut(s)	172, 569, 860
BsaWI	WCCGGW	2 cut(s)	182, 896
BsaXI	ACNNNNNCTCC	6 cut(s)	106, 136, 876, 906, 939, 969
Bsc4I	CCNNNNNNNGG	2 cut(s)	260, 686
Bse8I	GATNNNNATC	1 cut(s)	542
BseBI	CCWGG	4 cut(s)	510, 525, 552, 861
BseDI	CCNNGG	3 cut(s)	172, 569, 860
BseJI	GATNNNNATC	1 cut(s)	542
BseLI	CCNNNNNNNGG	2 cut(s)	260, 686
BseMII	CTCAG	2 cut(s)	309, 387
BseRI	GAGGAG	1 cut(s)	111
BseSI	GKGCMC	1 cut(s)	911
BseXI	GCAGC	9 cut(s)	41, 69, 75, 352, 355, 358, 379, 448, 778
BsgI	GTGCAG	1 cut(s)	837
BshFI	GGCC	1 cut(s)	498
BshNI	GGYRCC	1 cut(s)	227
BsiSI	CCGG	5 cut(s)	45, 183, 569, 843, 897
BslFI	GGGAC	4 cut(s)	127, 208, 703, 784
BslI	CCNNNNNNNGG	2 cut(s)	260, 686
BsmAI	GTCTC	4 cut(s)	13, 105, 314, 933
BsmBI	CGTCTC	1 cut(s)	933
BsmFI	GGGAC	4 cut(s)	127, 208, 703, 784
BsmI	GAATGC	1 cut(s)	536
BsnI	GGCC	1 cut(s)	498
Bsp1286I	GDGCHC	1 cut(s)	911
Bsp143I	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
BspACI	CCGC	9 cut(s)	48, 51, 60, 125, 134, 137, 789, 881, 983
BspANI	GGCC	1 cut(s)	498
BspCNI	CTCAG	2 cut(s)	310, 388
BspLI	GGNNCC	4 cut(s)	196, 229, 678, 886
BspMAI	CTGCAG	3 cut(s)	59, 637, 778
BspMI	ACCTGC	1 cut(s)	361
BspPI	GGATC	4 cut(s)	37, 551, 671, 684
BspT107I	GGYRCC	1 cut(s)	227
BssECI	CCNNGG	3 cut(s)	172, 569, 860
BssMI	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
BssNI	GRCGYC	1 cut(s)	865
BssSI	CACGAG	1 cut(s)	16
BssT1I	CCWWGG	1 cut(s)	172
Bst2BI	CACGAG	1 cut(s)	16
Bst2UI	CCWGG	4 cut(s)	510, 525, 552, 861
Bst4CI	ACNGT	3 cut(s)	99, 301, 473
Bst6I	CTCTTC	1 cut(s)	39
BstACI	GRCGYC	1 cut(s)	865
BstBAI	YACGTR	2 cut(s)	161, 349
BstDEI	CTNAG	5 cut(s)	312, 318, 396, 935, 972
BstHHI	GCGC	1 cut(s)	358
BstKTI	GATC	6 cut(s)	32, 345, 406, 546, 596, 679
BstMAI	GTCTC	4 cut(s)	13, 105, 314, 933
BstMBI	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
BstMWI	GCNNNNNNNGC	8 cut(s)	45, 54, 57, 60, 63, 362, 371, 839
BstNI	CCWGG	4 cut(s)	510, 525, 552, 861
BstSCI	CCNGG	5 cut(s)	508, 523, 550, 568, 859
BstSFI	CTRYAG	4 cut(s)	55, 633, 774, 825
BstSLI	GKGCMC	1 cut(s)	911
BstSNI	TACGTA	1 cut(s)	349
BstV1I	GCAGC	9 cut(s)	41, 69, 75, 352, 355, 358, 379, 448, 778
BstX2I	RGATCY	3 cut(s)	29, 403, 676
BstYI	RGATCY	3 cut(s)	29, 403, 676
BsuI	GTATCC	1 cut(s)	548
BsuRI	GGCC	1 cut(s)	498
BtgZI	GCGATG	1 cut(s)	1001
BtsIMutI	CAGTG	3 cut(s)	204, 297, 420
BveI	ACCTGC	1 cut(s)	361
CfoI	GCGC	1 cut(s)	358
Cfr13I	GGNCC	2 cut(s)	195, 497
CseI	GACGC	2 cut(s)	854, 950
CsiI	ACCWGGT	1 cut(s)	523
Csp6I	GTAC	3 cut(s)	95, 350, 813
CviAII	CATG	5 cut(s)	379, 440, 445, 547, 878
CviQI	GTAC	3 cut(s)	95, 350, 813
DdeI	CTNAG	5 cut(s)	312, 318, 396, 935, 972
DpnI	GATC	6 cut(s)	31, 344, 405, 545, 595, 678
DpnII	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
Eam1104I	CTCTTC	1 cut(s)	39
EarI	CTCTTC	1 cut(s)	39
EciI	GGCGGA	1 cut(s)	114
Eco105I	TACGTA	1 cut(s)	349
Eco130I	CCWWGG	1 cut(s)	172
Eco47I	GGWCC	1 cut(s)	195
Eco57I	CTGAAG	2 cut(s)	327, 444
Eco72I	CACGTG	1 cut(s)	161
EcoO109I	RGGNCCY	1 cut(s)	195
EcoRI	GAATTC	1 cut(s)	429
EcoRII	CCWGG	4 cut(s)	508, 523, 550, 859
EcoT14I	CCWWGG	1 cut(s)	172
ErhI	CCWWGG	1 cut(s)	172
Esp3I	CGTCTC	1 cut(s)	933
FaeI	CATG	5 cut(s)	382, 443, 448, 550, 881
FaqI	GGGAC	4 cut(s)	127, 208, 703, 784
FatI	CATG	5 cut(s)	378, 439, 444, 546, 877
FauI	CCCGC	1 cut(s)	874
FauNDI	CATATG	1 cut(s)	610
FblI	GTMKAC	1 cut(s)	147
FspBI	CTAG	2 cut(s)	6, 486
GlaI	GCGC	1 cut(s)	357
HaeIII	GGCC	1 cut(s)	498
HapII	CCGG	5 cut(s)	45, 183, 569, 843, 897
HgaI	GACGC	2 cut(s)	854, 950
HhaI	GCGC	1 cut(s)	358
Hin1I	GRCGYC	1 cut(s)	865
Hin1II	CATG	5 cut(s)	382, 443, 448, 550, 881
Hin6I	GCGC	1 cut(s)	356
HinP1I	GCGC	1 cut(s)	356
HincII	GTYRAC	3 cut(s)	148, 703, 727
HindII	GTYRAC	3 cut(s)	148, 703, 727
HinfI	GANTC	1 cut(s)	962
HpaII	CCGG	5 cut(s)	45, 183, 569, 843, 897
HphI	GGTGA	2 cut(s)	539, 957
Hpy166II	GTNNAC	3 cut(s)	148, 703, 727
Hpy188I	TCNGA	1 cut(s)	29
Hpy188III	TCNNGA	5 cut(s)	16, 112, 591, 597, 917
Hpy8I	GTNNAC	3 cut(s)	148, 703, 727
Hpy99I	CGWCG	1 cut(s)	870
HpyAV	CCTTC	6 cut(s)	118, 208, 253, 377, 419, 734
HpyCH4III	ACNGT	3 cut(s)	99, 301, 473
HpyCH4IV	ACGT	2 cut(s)	160, 348
HpyCH4V	TGCA	5 cut(s)	57, 534, 635, 776, 854
HpyF10VI	GCNNNNNNNGC	8 cut(s)	45, 54, 57, 60, 63, 362, 371, 839
HpyF3I	CTNAG	5 cut(s)	312, 318, 396, 935, 972
HpySE526I	ACGT	2 cut(s)	160, 348
Hsp92I	GRCGYC	1 cut(s)	865
Hsp92II	CATG	5 cut(s)	382, 443, 448, 550, 881
HspAI	GCGC	1 cut(s)	356
Kzo9I	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
LmnI	GCTCC	2 cut(s)	110, 884
Lsp1109I	GCAGC	9 cut(s)	41, 69, 75, 352, 355, 358, 379, 448, 778
LweI	GCATC	1 cut(s)	775
MabI	ACCWGGT	1 cut(s)	523
MaeI	CTAG	2 cut(s)	6, 486
MaeII	ACGT	2 cut(s)	160, 348
MaeIII	GTNAC	3 cut(s)	13, 247, 963
MalI	GATC	6 cut(s)	31, 344, 405, 545, 595, 678
MboI	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
MboII	GAAGA	4 cut(s)	26, 586, 791, 794
MflI	RGATCY	3 cut(s)	29, 403, 676
MhlI	GDGCHC	1 cut(s)	911
MluCI	AATT	4 cut(s)	429, 502, 666, 694
MlyI	GAGTC	1 cut(s)	971
MnlI	CCTC	5 cut(s)	132, 138, 346, 444, 1004
MroXI	GAANNNNTTC	1 cut(s)	429
MseI	TTAA	3 cut(s)	468, 684, 951
MslI	CAYNNNNRTG	1 cut(s)	233
MspA1I	CMGCKG	2 cut(s)	60, 791
MspI	CCGG	5 cut(s)	45, 183, 569, 843, 897
MspR9I	CCNGG	5 cut(s)	510, 525, 552, 570, 861
Mva1269I	GAATGC	1 cut(s)	536
MvaI	CCWGG	4 cut(s)	510, 525, 552, 861
MwoI	GCNNNNNNNGC	8 cut(s)	45, 54, 57, 60, 63, 362, 371, 839
NciI	CCSGG	1 cut(s)	570
NdeI	CATATG	1 cut(s)	610
NdeII	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
NlaIII	CATG	5 cut(s)	382, 443, 448, 550, 881
NlaIV	GGNNCC	4 cut(s)	196, 229, 678, 886
NmuCI	GTSAC	3 cut(s)	13, 247, 963
PctI	GAATGC	1 cut(s)	536
PdmI	GAANNNNTTC	1 cut(s)	429
PleI	GAGTC	1 cut(s)	970
PmaCI	CACGTG	1 cut(s)	161
PmlI	CACGTG	1 cut(s)	161
PpsI	GAGTC	1 cut(s)	970
Ppu21I	YACGTR	2 cut(s)	161, 349
PpuMI	RGGWCCY	1 cut(s)	195
Psp5II	RGGWCCY	1 cut(s)	195
Psp6I	CCWGG	4 cut(s)	508, 523, 550, 859
PspCI	CACGTG	1 cut(s)	161
PspGI	CCWGG	4 cut(s)	508, 523, 550, 859
PspN4I	GGNNCC	4 cut(s)	196, 229, 678, 886
PspPI	GGNCC	2 cut(s)	195, 497
PspPPI	RGGWCCY	1 cut(s)	195
PstI	CTGCAG	3 cut(s)	59, 637, 778
PsuI	RGATCY	3 cut(s)	29, 403, 676
RsaI	GTAC	3 cut(s)	96, 351, 814
RsaNI	GTAC	3 cut(s)	95, 350, 813
RseI	CAYNNNNRTG	1 cut(s)	233
SalI	GTCGAC	1 cut(s)	146
SaqAI	TTAA	3 cut(s)	468, 684, 951
Sau3AI	GATC	6 cut(s)	29, 342, 403, 543, 593, 676
Sau96I	GGNCC	2 cut(s)	195, 497
SchI	GAGTC	1 cut(s)	971
ScrFI	CCNGG	5 cut(s)	510, 525, 552, 570, 861
SduI	GDGCHC	1 cut(s)	911
SexAI	ACCWGGT	1 cut(s)	523
SfaNI	GCATC	1 cut(s)	775
SfcI	CTRYAG	4 cut(s)	55, 633, 774, 825
SinI	GGWCC	1 cut(s)	195
SmiMI	CAYNNNNRTG	1 cut(s)	233
SmlI	CTYRAG	1 cut(s)	915
SmoI	CTYRAG	1 cut(s)	915
SnaBI	TACGTA	1 cut(s)	349
Sse9I	AATT	4 cut(s)	429, 502, 666, 694
SsiI	CCGC	9 cut(s)	48, 51, 60, 125, 134, 137, 789, 881, 983
SspMI	CTAG	2 cut(s)	6, 486
StyD4I	CCNGG	5 cut(s)	508, 523, 550, 568, 859
StyI	CCWWGG	1 cut(s)	172
TaaI	ACNGT	3 cut(s)	99, 301, 473
TaiI	ACGT	2 cut(s)	163, 351
TaqI	TCGA	2 cut(s)	147, 592
TasI	AATT	4 cut(s)	429, 502, 666, 694
TauI	GCSGC	6 cut(s)	51, 54, 63, 137, 791, 986
Tru1I	TTAA	3 cut(s)	468, 684, 951
Tru9I	TTAA	3 cut(s)	468, 684, 951
TscAI	CASTG	3 cut(s)	211, 304, 427
TseFI	GTSAC	3 cut(s)	13, 247, 963
TseI	GCWGC	9 cut(s)	54, 57, 63, 365, 368, 371, 392, 461, 791
Tsp45I	GTSAC	3 cut(s)	13, 247, 963
TspDTI	ATGAA	3 cut(s)	78, 627, 826
TspGWI	ACGGA	1 cut(s)	669
TspRI	CASTG	3 cut(s)	211, 304, 427
VpaK11BI	GGWCC	1 cut(s)	195
XapI	RAATTY	2 cut(s)	429, 694
XmiI	GTMKAC	1 cut(s)	147
XmnI	GAANNNNTTC	1 cut(s)	429
XspI	CTAG	2 cut(s)	6, 486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.