Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009184

Physical Location & Seq

Genomic Coordinates Forward (+)

36992753 .. 36996311

Gene Structure 3559 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1239800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGGTCGCCAAGCCGTAGCCAAGCTGATCGGTTCGATCGCATCGCGCAAACCATCTCCTTCAACGTCTTTACGTCAGCGCCGTCATTACTCCCCGGCTCCGCCCCCGCCCCCCGCCGTCTTCGTCGACAAAAACACTCGCGTCATATGCCAAGGCATCACCGGAAAGAACGGCACTTTCCACACCGAGCAAGCCATCGAATATGGCACCAAAATGGTGGGTGGTGTAACACCGAAGAAGGGTGGGACTGAGCATTTGGGACTTCCTGTTTTCAACTCAGTTGCAGAAGCAAAAGCTGAAACAAAGGCTAATGCCTCAGTCATATATGTTCCCCCACCATTTGCTGCTGCTGCTATTCTGGAGGCAGTGGAAGCAGAGCTGGATCTTGTTGTCTGCATTACTGAGGGGATCCCACAACATGATATGGTGCGTGTCAAGGCTGCTATTAACAGGCAATCTAAAACTCGCCTCATTGGGCCAAACTGCCCTGGAATTATCAAGCCTGGTGAATGCAAAATCGGAATTATGCCTGGCTACATTCATAAACCTGGGCGCATAGGAATTGTTTCTCGATCTGGTACCCTGACCTATGAAGCGGTTTTCCAAACAACTGCTGTTGGCCTTGGACAATCCACCTGTGTTGGTATTGGGGGGGATCCGTTTAATGGTACAAACTTTGTTGATTGCATTGACAGATTCCTACAAGATCCTCAAACAGAAGGTATCATTCTTATAGGAGAGATTGGTGGGACTGCAGAAGAGGATGCTGCTGCATTGATTAAGGAAAGTGGAACTGAGAAGCCTGTTGTTGCATTTATTGCTGGATTAACTGCTCCTCCTGGTCGTCGAATGGGTCATGCTGGAGCTATTGTGTCAGGGGGAAAGGGTACAGCACAAGACAAAATCAAGACACTAAGGGAAGCTGGGGTTACAGTGGTGGAGTCTCCTGCGAAAATTGGGGCGGCCATGCTTGACGTCTTCAAACAAAGGGGCCTTGTGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004774 GO:0004775 GO:0004776 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006104 GO:0006105 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019637 GO:0019693 GO:0019752 GO:0030312 GO:0031974 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046483 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0071944 GO:0072350 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00640 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

CoA_binding Ligase_CoA

Protein Analysis

335

Amino Acids

34.86

Weight (kDa)

9.18

Isoelectric Point (pI)

42.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CoA_binding	PF02629	43 - 136	2.5e-33	CoA binding domain
Succ_CoA_lig	PF13607	183 - 317	4.5e-11	Succinyl-CoA ligase like flavodoxin domain
Ligase_CoA	PF00549	189 - 310	1e-30	CoA-ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08300 AT5G23250 AT5G23250 AT5G23250
fragaria_vesca	FvH4_5g18391
malus_domestica	MD06G1239800.v1.1 MD14G1245700.v1.1
prunus_persica	Prupe.5G243700_v2.0.a1 Prupe.5G243700_v2.0.a1
pyrus_communis	pycom06g21530 pycom14g20660
rosa_chinensis	RchiOBHm_Chr7g0177081
rosa_laevigata	RLG00000005558
rosa_multiflora	Rmu_sc0005310.1_g000024 Rmu_sc0005310.1_g000025 Rmu_sc0005912.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276110
rosa_rugosa	Rorug06G0403600
rosa_samantha	Rh7AG003000 Rh7CG002600 Rh7DG003100
rosa_wichuraiana	Rw7G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	978
Acc65I	GGTACC	1 cut(s)	578
AccB1I	GGYRCC	2 cut(s)	206, 578
AccI	GTMKAC	1 cut(s)	126
AccII	CGCG	2 cut(s)	46, 141
AciI	CCGC	5 cut(s)	101, 107, 114, 596, 962
AclWI	GGATC	6 cut(s)	390, 403, 416, 650, 663, 701
AcoI	YGGCCR	1 cut(s)	963
AcyI	GRCGYC	1 cut(s)	975
AfaI	GTAC	3 cut(s)	580, 670, 889
AfiI	CCNNNNNNNGG	2 cut(s)	239, 665
AgsI	TTSAA	3 cut(s)	63, 274, 982
AjnI	CCWGG	5 cut(s)	487, 502, 529, 547, 838
AluBI	AGCT	5 cut(s)	25, 296, 379, 866, 923
AluI	AGCT	5 cut(s)	25, 296, 379, 866, 923
Alw26I	GTCTC	1 cut(s)	948
AlwI	GGATC	6 cut(s)	390, 403, 416, 650, 663, 701
AoxI	GGCC	4 cut(s)	476, 619, 963, 991
ApeKI	GCWGC	6 cut(s)	344, 347, 350, 440, 767, 770
ArsI	GACNNNNNNTTYG	2 cut(s)	238, 270
Asp700I	GAANNNNTTC	1 cut(s)	565
Asp718I	GGTACC	1 cut(s)	578
AspLEI	GCGC	3 cut(s)	48, 81, 555
AspS9I	GGNCC	2 cut(s)	476, 991
AsuC2I	CCSGG	1 cut(s)	95
AsuHPI	GGTGA	2 cut(s)	151, 518
BamHI	GGATCC	2 cut(s)	408, 655
BanI	GGYRCC	2 cut(s)	206, 578
BbsI	GAAGAC	2 cut(s)	112, 970
BbvI	GCAGC	6 cut(s)	331, 334, 337, 427, 754, 757
BccI	CCATC	2 cut(s)	61, 203
BceAI	ACGGC	3 cut(s)	66, 101, 187
BciT130I	CCWGG	5 cut(s)	489, 504, 531, 549, 840
BcnI	CCSGG	1 cut(s)	95
BcoDI	GTCTC	1 cut(s)	948
BfmI	CTRYAG	1 cut(s)	753
BfoI	RGCGCY	1 cut(s)	82
BisI	GCNGC	7 cut(s)	345, 348, 351, 441, 768, 771, 963
BlsI	GCNGC	7 cut(s)	346, 349, 352, 442, 769, 772, 964
Bme1390I	CCNGG	6 cut(s)	95, 489, 504, 531, 549, 840
BmgT120I	GGNCC	2 cut(s)	476, 991
BmiI	GGNNCC	6 cut(s)	99, 208, 410, 580, 657, 992
BmrFI	CCNGG	6 cut(s)	95, 489, 504, 531, 549, 840
BmsI	GCATC	3 cut(s)	50, 165, 754
BpiI	GAAGAC	2 cut(s)	112, 970
BpmI	CTGGAG	2 cut(s)	380, 882
BpuMI	CCSGG	1 cut(s)	95
BsaHI	GRCGYC	1 cut(s)	975
BsaJI	CCNNGG	5 cut(s)	93, 151, 487, 548, 622
BsaWI	WCCGGW	1 cut(s)	161
BsaXI	ACNNNNNCTCC	4 cut(s)	820, 850, 855, 885
Bsc4I	CCNNNNNNNGG	2 cut(s)	239, 665
BseBI	CCWGG	5 cut(s)	489, 504, 531, 549, 840
BseDI	CCNNGG	5 cut(s)	93, 151, 487, 548, 622
BseGI	GGATG	1 cut(s)	769
BseLI	CCNNNNNNNGG	2 cut(s)	239, 665
BseMII	CTCAG	5 cut(s)	240, 291, 330, 393, 786
BseRI	GAGGAG	1 cut(s)	825
BseXI	GCAGC	6 cut(s)	331, 334, 337, 427, 754, 757
BseYI	CCCAGC	1 cut(s)	923
Bsh1236I	CGCG	2 cut(s)	46, 141
Bsh1285I	CGRYCG	1 cut(s)	39
BshFI	GGCC	4 cut(s)	478, 621, 965, 993
BshNI	GGYRCC	2 cut(s)	206, 578
BsiEI	CGRYCG	1 cut(s)	39
BsiSI	CCGG	2 cut(s)	95, 162
BslFI	GGGAC	3 cut(s)	259, 273, 763
BslI	CCNNNNNNNGG	2 cut(s)	239, 665
BsmAI	GTCTC	1 cut(s)	948
BsmFI	GGGAC	3 cut(s)	259, 273, 763
BsmI	GAATGC	1 cut(s)	515
BsnI	GGCC	4 cut(s)	478, 621, 965, 993
Bsp143I	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
BspACI	CCGC	5 cut(s)	101, 107, 114, 596, 962
BspANI	GGCC	4 cut(s)	478, 621, 965, 993
BspCNI	CTCAG	5 cut(s)	241, 290, 329, 394, 787
BspFNI	CGCG	2 cut(s)	46, 141
BspLI	GGNNCC	6 cut(s)	99, 208, 410, 580, 657, 992
BspMAI	CTGCAG	1 cut(s)	757
BspPI	GGATC	6 cut(s)	390, 403, 416, 650, 663, 701
BspT107I	GGYRCC	2 cut(s)	206, 578
BssECI	CCNNGG	5 cut(s)	93, 151, 487, 548, 622
BssMI	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
BssNI	GRCGYC	1 cut(s)	975
BssT1I	CCWWGG	2 cut(s)	151, 622
Bst2UI	CCWGG	5 cut(s)	489, 504, 531, 549, 840
Bst4CI	ACNGT	1 cut(s)	934
Bst6I	CTCTTC	1 cut(s)	753
BstACI	GRCGYC	1 cut(s)	975
BstAPI	GCANNNNNTGC	1 cut(s)	818
BstC8I	GCNNGC	1 cut(s)	192
BstDEI	CTNAG	6 cut(s)	249, 277, 316, 402, 795, 914
BstF5I	GGATG	1 cut(s)	769
BstFNI	CGCG	2 cut(s)	46, 141
BstH2I	RGCGCY	1 cut(s)	82
BstHHI	GCGC	3 cut(s)	48, 81, 555
BstKTI	GATC	7 cut(s)	30, 39, 385, 411, 575, 658, 709
BstMAI	GTCTC	1 cut(s)	948
BstMBI	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
BstMCI	CGRYCG	1 cut(s)	39
BstMWI	GCNNNNNNNGC	4 cut(s)	147, 350, 371, 818
BstNI	CCWGG	5 cut(s)	489, 504, 531, 549, 840
BstSCI	CCNGG	6 cut(s)	93, 487, 502, 529, 547, 838
BstSFI	CTRYAG	1 cut(s)	753
BstUI	CGCG	2 cut(s)	46, 141
BstV1I	GCAGC	6 cut(s)	331, 334, 337, 427, 754, 757
BstV2I	GAAGAC	2 cut(s)	112, 970
BstX2I	RGATCY	4 cut(s)	382, 408, 655, 706
BstXI	CCANNNNNNTGG	1 cut(s)	217
BstYI	RGATCY	4 cut(s)	382, 408, 655, 706
BsuRI	GGCC	4 cut(s)	478, 621, 965, 993
BtgZI	GCGATG	1 cut(s)	27
BtsCI	GGATG	1 cut(s)	769
BtsI	GCAGTG	1 cut(s)	372
BtsIMutI	CAGTG	2 cut(s)	372, 939
Cac8I	GCNNGC	1 cut(s)	192
CfoI	GCGC	3 cut(s)	48, 81, 555
Cfr13I	GGNCC	2 cut(s)	476, 991
CseI	GACGC	1 cut(s)	130
Csp6I	GTAC	3 cut(s)	579, 669, 888
CspCI	CAANNNNNGTGG	2 cut(s)	622, 657
CviAII	CATG	3 cut(s)	419, 857, 967
CviQI	GTAC	3 cut(s)	579, 669, 888
DdeI	CTNAG	6 cut(s)	249, 277, 316, 402, 795, 914
DpnI	GATC	7 cut(s)	29, 38, 384, 410, 574, 657, 708
DpnII	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
EaeI	YGGCCR	1 cut(s)	963
Eam1104I	CTCTTC	1 cut(s)	753
EarI	CTCTTC	1 cut(s)	753
EciI	GGCGGA	1 cut(s)	90
Eco130I	CCWWGG	2 cut(s)	151, 622
EcoO109I	RGGNCCY	1 cut(s)	991
EcoRII	CCWGG	5 cut(s)	487, 502, 529, 547, 838
EcoT14I	CCWWGG	2 cut(s)	151, 622
ErhI	CCWWGG	2 cut(s)	151, 622
FaeI	CATG	3 cut(s)	422, 860, 970
FaqI	GGGAC	3 cut(s)	259, 273, 763
FatI	CATG	3 cut(s)	418, 856, 966
FauI	CCCGC	2 cut(s)	114, 121
FauNDI	CATATG	1 cut(s)	146
FblI	GTMKAC	1 cut(s)	126
Fnu4HI	GCNGC	7 cut(s)	345, 348, 351, 441, 768, 771, 963
FokI	GGATG	1 cut(s)	776
Fsp4HI	GCNGC	7 cut(s)	345, 348, 351, 441, 768, 771, 963
GlaI	GCGC	3 cut(s)	47, 80, 554
GluI	GCNGC	7 cut(s)	345, 348, 351, 441, 768, 771, 963
GsaI	CCCAGC	1 cut(s)	927
GsuI	CTGGAG	2 cut(s)	380, 882
HaeII	RGCGCY	1 cut(s)	82
HaeIII	GGCC	4 cut(s)	478, 621, 965, 993
HapII	CCGG	2 cut(s)	95, 162
HgaI	GACGC	1 cut(s)	130
HhaI	GCGC	3 cut(s)	48, 81, 555
Hin1I	GRCGYC	1 cut(s)	975
Hin1II	CATG	3 cut(s)	422, 860, 970
Hin6I	GCGC	3 cut(s)	46, 79, 553
HinP1I	GCGC	3 cut(s)	46, 79, 553
HincII	GTYRAC	1 cut(s)	127
HindII	GTYRAC	1 cut(s)	127
HinfI	GANTC	2 cut(s)	696, 941
HpaII	CCGG	2 cut(s)	95, 162
HphI	GGTGA	2 cut(s)	151, 518
Hpy166II	GTNNAC	1 cut(s)	127
Hpy188I	TCNGA	1 cut(s)	521
Hpy188III	TCNNGA	3 cut(s)	359, 570, 907
Hpy8I	GTNNAC	1 cut(s)	127
Hpy99I	CGWCG	2 cut(s)	128, 849
HpyAV	CCTTC	3 cut(s)	69, 232, 713
HpyCH4III	ACNGT	1 cut(s)	934
HpyCH4IV	ACGT	3 cut(s)	65, 73, 975
HpyCH4V	TGCA	7 cut(s)	284, 396, 513, 687, 755, 773, 812
HpyF10VI	GCNNNNNNNGC	4 cut(s)	147, 350, 371, 818
HpyF3I	CTNAG	6 cut(s)	249, 277, 316, 402, 795, 914
HpySE526I	ACGT	3 cut(s)	65, 73, 975
Hsp92I	GRCGYC	1 cut(s)	975
Hsp92II	CATG	3 cut(s)	422, 860, 970
HspAI	GCGC	3 cut(s)	46, 79, 553
KpnI	GGTACC	1 cut(s)	582
Kzo9I	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
LmnI	GCTCC	3 cut(s)	103, 838, 863
Lsp1109I	GCAGC	6 cut(s)	331, 334, 337, 427, 754, 757
LweI	GCATC	3 cut(s)	50, 165, 754
MaeII	ACGT	3 cut(s)	65, 73, 975
MaeIII	GTNAC	2 cut(s)	226, 928
MalI	GATC	7 cut(s)	29, 38, 384, 410, 574, 657, 708
MboI	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
MboII	GAAGA	4 cut(s)	112, 247, 770, 970
MflI	RGATCY	4 cut(s)	382, 408, 655, 706
MluCI	AATT	5 cut(s)	492, 522, 561, 954, 1000
MlyI	GAGTC	1 cut(s)	950
MnlI	CCTC	7 cut(s)	325, 355, 397, 479, 720, 754, 846
MroXI	GAANNNNTTC	1 cut(s)	565
MseI	TTAA	4 cut(s)	447, 663, 780, 827
MslI	CAYNNNNRTG	1 cut(s)	212
MspI	CCGG	2 cut(s)	95, 162
MspR9I	CCNGG	6 cut(s)	95, 489, 504, 531, 549, 840
Mva1269I	GAATGC	1 cut(s)	515
MvaI	CCWGG	5 cut(s)	489, 504, 531, 549, 840
MvnI	CGCG	2 cut(s)	46, 141
MwoI	GCNNNNNNNGC	4 cut(s)	147, 350, 371, 818
NciI	CCSGG	1 cut(s)	95
NdeI	CATATG	1 cut(s)	146
NdeII	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
NlaIII	CATG	3 cut(s)	422, 860, 970
NlaIV	GGNNCC	6 cut(s)	99, 208, 410, 580, 657, 992
PcsI	WCGNNNNNNNCGW	1 cut(s)	79
PctI	GAATGC	1 cut(s)	515
PdmI	GAANNNNTTC	1 cut(s)	565
PfeI	GAWTC	1 cut(s)	696
PkrI	GCNGC	7 cut(s)	346, 349, 352, 442, 769, 772, 964
Ple19I	CGATCG	1 cut(s)	39
PleI	GAGTC	1 cut(s)	949
PpsI	GAGTC	1 cut(s)	949
Psp6I	CCWGG	5 cut(s)	487, 502, 529, 547, 838
PspFI	CCCAGC	1 cut(s)	923
PspGI	CCWGG	5 cut(s)	487, 502, 529, 547, 838
PspN4I	GGNNCC	6 cut(s)	99, 208, 410, 580, 657, 992
PspPI	GGNCC	2 cut(s)	476, 991
PstI	CTGCAG	1 cut(s)	757
PsuI	RGATCY	4 cut(s)	382, 408, 655, 706
PvuI	CGATCG	1 cut(s)	39
RsaI	GTAC	3 cut(s)	580, 670, 889
RsaNI	GTAC	3 cut(s)	579, 669, 888
RseI	CAYNNNNRTG	1 cut(s)	212
SalI	GTCGAC	1 cut(s)	125
SaqAI	TTAA	4 cut(s)	447, 663, 780, 827
SatI	GCNGC	7 cut(s)	345, 348, 351, 441, 768, 771, 963
Sau3AI	GATC	7 cut(s)	27, 36, 382, 408, 572, 655, 706
Sau96I	GGNCC	2 cut(s)	476, 991
SchI	GAGTC	1 cut(s)	950
ScrFI	CCNGG	6 cut(s)	95, 489, 504, 531, 549, 840
SfaNI	GCATC	3 cut(s)	50, 165, 754
SfcI	CTRYAG	1 cut(s)	753
SmiMI	CAYNNNNRTG	1 cut(s)	212
Sse9I	AATT	5 cut(s)	492, 522, 561, 954, 1000
SsiI	CCGC	5 cut(s)	101, 107, 114, 596, 962
StyD4I	CCNGG	6 cut(s)	93, 487, 502, 529, 547, 838
StyI	CCWWGG	2 cut(s)	151, 622
TaaI	ACNGT	1 cut(s)	934
TaiI	ACGT	3 cut(s)	68, 76, 978
TaqI	TCGA	5 cut(s)	35, 126, 198, 571, 847
TasI	AATT	5 cut(s)	492, 522, 561, 954, 1000
TauI	GCSGC	1 cut(s)	965
TfiI	GAWTC	1 cut(s)	696
Tru1I	TTAA	4 cut(s)	447, 663, 780, 827
Tru9I	TTAA	4 cut(s)	447, 663, 780, 827
TscAI	CASTG	2 cut(s)	372, 939
TseI	GCWGC	6 cut(s)	344, 347, 350, 440, 767, 770
TspDTI	ATGAA	2 cut(s)	530, 606
TspGWI	ACGGA	1 cut(s)	648
TspRI	CASTG	2 cut(s)	372, 939
XmiI	GTMKAC	1 cut(s)	126
XmnI	GAANNNNTTC	1 cut(s)	565
ZraI	GACGTC	1 cut(s)	976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.