Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0009184

Physical Location & Seq

Genomic Coordinates Forward (+)

10570607 .. 10573990

Gene Structure 3384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g18391.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1014 bp

ATGGCTCGCCAATCCGTTGCCAAGCTGATCGGCTCGATCGCATCACGCAAGCTTTCATCACCATCTACGTCAACGACGTCAATTATTCAGCGCCGTCACTACGCCGCCGCCCCGCCGCCCCCGGCTGTCTTCGTCGACAAAAACACTCGCGTCATTTGCCAGGGAATCACAGGAAAGAACGGCACCTTCCACACCGAACAAGCCATCGAGTATGGCACTAAAATGGTGGGTGGTGTTACTCCAAAGAAGGGCGGGACCGAGCATTTGGGACTTCCAGTCTTCAATTCAGTGGTGGAAGCAAAGGCCGAAACAAAGGCTAATGCAACGGTGATCTATGTTCCTCCACCATTTGCTGCCGCTGCAATTATGGAAGCAATGGAAGCTGAGCTGGATCTTGTTGTGTGCATCACTGAGGGGATCCCGCAACATGATATGGTACGTGTGAAAGCTGCACTTAACAGCCAATCTAAAACTCGGCTCATTGGACCAAATTGCCCTGGAATTATCAAGCCTGGTGAATGCAAAATTGGAATTATGCCTGGCTACATTCATAAACCAGGTCGTGTGGGAATTGTTTCTCGATCTGGTACCTTGACTTATGAAGCGGTTTTCCAAACAACTGCCGTTGGCCTTGGACAATCCACTTGCGTTGGTATTGGGGGTGATCCTTTCAATGGTACAAACTTTGTTGATTGCATGGAAAAATTCTTAGCAGATCCTCAGACAGAAGGTATTGTTCTGATAGGAGAGATTGGTGGGACTGCAGAAGAGGATGCCGCCGCATTGATAAAGGCAAGTGGAACTGAGAAGCCAATTGTTGCATTTATTGCTGGACTAACTGCTCCCCCTGGTCGGCGTATGGGTCATGCAGGAGCTATTGTATCGGGTGGAAAGGGTACCGCACAAGATAAAATCAAGACTCTTAGAGAAGCTGGGGTTACAGTGGTGGAGTCTCCGGCAAAAATTGGGGTTGCCATGCTTGATGTGTTCAAGCAGAGGGGACTAGTGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004774 GO:0004775 GO:0004776 GO:0005488 GO:0005507 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006104 GO:0006105 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019637 GO:0019693 GO:0019752 GO:0030312 GO:0031974 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043603 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046483 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0070013 GO:0071704 GO:0071944 GO:0072350 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00640 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

CoA_binding Ligase_CoA

Protein Analysis

338

Amino Acids

35.03

Weight (kDa)

9.06

Isoelectric Point (pI)

37.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CoA_binding	PF02629	46 - 139	1e-32	CoA binding domain
Succ_CoA_lig	PF13607	186 - 320	3.2e-11	Succinyl-CoA ligase like flavodoxin domain
Ligase_CoA	PF00549	192 - 313	2.4e-30	CoA-ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009184)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08300 AT5G23250 AT5G23250 AT5G23250
fragaria_vesca	FvH4_5g18391
malus_domestica	MD06G1239800.v1.1 MD14G1245700.v1.1
prunus_persica	Prupe.5G243700_v2.0.a1 Prupe.5G243700_v2.0.a1
pyrus_communis	pycom06g21530 pycom14g20660
rosa_chinensis	RchiOBHm_Chr7g0177081
rosa_laevigata	RLG00000005558
rosa_multiflora	Rmu_sc0005310.1_g000024 Rmu_sc0005310.1_g000025 Rmu_sc0005912.1_g000001
rosa_roxburghii	Rroxscaffold_3G00276110
rosa_rugosa	Rorug06G0403600
rosa_samantha	Rh7AG003000 Rh7CG002600 Rh7DG003100
rosa_wichuraiana	Rw7G000250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	80
Acc65I	GGTACC	2 cut(s)	587, 896
AccB1I	GGYRCC	3 cut(s)	182, 587, 896
AccI	GTMKAC	1 cut(s)	135
AccII	CGCG	1 cut(s)	150
AclWI	GGATC	5 cut(s)	399, 412, 425, 659, 710
AcsI	RAATTY	1 cut(s)	704
AcyI	GRCGYC	1 cut(s)	77
AfaI	GTAC	4 cut(s)	438, 589, 679, 898
AfiI	CCNNNNNNNGG	3 cut(s)	248, 674, 852
AflIII	ACRYGT	1 cut(s)	439
AgsI	TTSAA	3 cut(s)	283, 673, 991
AhdI	GACNNNNNGTC	1 cut(s)	275
AhlI	ACTAGT	1 cut(s)	1003
AjnI	CCWGG	6 cut(s)	159, 496, 511, 538, 556, 847
AjuI	GAANNNNNNNTTGG	2 cut(s)	510, 542
AluBI	AGCT	7 cut(s)	25, 52, 383, 388, 449, 875, 932
AluI	AGCT	7 cut(s)	25, 52, 383, 388, 449, 875, 932
Alw26I	GTCTC	1 cut(s)	957
AlwI	GGATC	5 cut(s)	399, 412, 425, 659, 710
AoxI	GGCC	2 cut(s)	303, 628
ApeKI	GCWGC	3 cut(s)	353, 359, 449
ApoI	RAATTY	1 cut(s)	704
ArsI	GACNNNNNNTTYG	2 cut(s)	247, 279
Asp700I	GAANNNNTTC	1 cut(s)	574
Asp718I	GGTACC	2 cut(s)	587, 896
AspLEI	GCGC	1 cut(s)	93
AspS9I	GGNCC	2 cut(s)	255, 485
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	4 cut(s)	51, 340, 527, 674
AvaII	GGWCC	2 cut(s)	255, 485
BamHI	GGATCC	1 cut(s)	417
BanI	GGYRCC	3 cut(s)	182, 587, 896
BbsI	GAAGAC	2 cut(s)	121, 271
BbvI	GCAGC	3 cut(s)	340, 346, 436
BccI	CCATC	2 cut(s)	70, 212
BceAI	ACGGC	3 cut(s)	78, 196, 608
BciT130I	CCWGG	6 cut(s)	161, 498, 513, 540, 558, 849
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	1 cut(s)	957
BcuI	ACTAGT	1 cut(s)	1003
BfaI	CTAG	2 cut(s)	1004, 1012
BfmI	CTRYAG	1 cut(s)	762
BfoI	RGCGCY	1 cut(s)	94
BisI	GCNGC	9 cut(s)	105, 108, 116, 354, 357, 360, 450, 777, 780
BlpI	GCTNAGC	1 cut(s)	384
BlsI	GCNGC	9 cut(s)	106, 109, 117, 355, 358, 361, 451, 778, 781
Bme1390I	CCNGG	7 cut(s)	122, 161, 498, 513, 540, 558, 849
Bme18I	GGWCC	2 cut(s)	255, 485
BmeRI	GACNNNNNGTC	1 cut(s)	275
BmgT120I	GGNCC	2 cut(s)	255, 485
BmiI	GGNNCC	5 cut(s)	184, 256, 419, 589, 898
BmrFI	CCNGG	7 cut(s)	122, 161, 498, 513, 540, 558, 849
BmsI	GCATC	3 cut(s)	50, 414, 763
BpiI	GAAGAC	2 cut(s)	121, 271
Bpu1102I	GCTNAGC	1 cut(s)	384
BpuMI	CCSGG	1 cut(s)	122
BsaAI	YACGTR	1 cut(s)	440
BsaHI	GRCGYC	1 cut(s)	77
BsaJI	CCNNGG	5 cut(s)	120, 160, 496, 631, 847
BsaXI	ACNNNNNCTCC	2 cut(s)	864, 894
Bsc4I	CCNNNNNNNGG	3 cut(s)	248, 674, 852
Bse1I	ACTGG	1 cut(s)	275
Bse3DI	GCAATG	1 cut(s)	381
BseBI	CCWGG	6 cut(s)	161, 498, 513, 540, 558, 849
BseDI	CCNNGG	5 cut(s)	120, 160, 496, 631, 847
BseGI	GGATG	1 cut(s)	778
BseLI	CCNNNNNNNGG	3 cut(s)	248, 674, 852
BseMI	GCAATG	1 cut(s)	381
BseMII	CTCAG	4 cut(s)	375, 402, 734, 795
BseNI	ACTGG	1 cut(s)	275
BseXI	GCAGC	3 cut(s)	340, 346, 436
BseYI	CCCAGC	1 cut(s)	932
BsgI	GTGCAG	1 cut(s)	435
Bsh1236I	CGCG	1 cut(s)	150
Bsh1285I	CGRYCG	1 cut(s)	39
BshFI	GGCC	2 cut(s)	305, 630
BshNI	GGYRCC	3 cut(s)	182, 587, 896
BsiEI	CGRYCG	1 cut(s)	39
BsiSI	CCGG	2 cut(s)	122, 956
BslFI	GGGAC	3 cut(s)	268, 282, 772
BslI	CCNNNNNNNGG	3 cut(s)	248, 674, 852
BsmAI	GTCTC	1 cut(s)	957
BsmFI	GGGAC	3 cut(s)	268, 282, 772
BsmI	GAATGC	1 cut(s)	524
BsnI	GGCC	2 cut(s)	305, 630
Bsp143I	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
Bsp1720I	GCTNAGC	1 cut(s)	384
BspANI	GGCC	2 cut(s)	305, 630
BspCNI	CTCAG	4 cut(s)	376, 403, 733, 796
BspFNI	CGCG	1 cut(s)	150
BspLI	GGNNCC	5 cut(s)	184, 256, 419, 589, 898
BspMAI	CTGCAG	1 cut(s)	766
BspPI	GGATC	5 cut(s)	399, 412, 425, 659, 710
BspT107I	GGYRCC	3 cut(s)	182, 587, 896
BsrDI	GCAATG	1 cut(s)	381
BsrI	ACTGG	1 cut(s)	275
BssECI	CCNNGG	5 cut(s)	120, 160, 496, 631, 847
BssMI	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
BssNI	GRCGYC	1 cut(s)	77
BssT1I	CCWWGG	1 cut(s)	631
Bst2UI	CCWGG	6 cut(s)	161, 498, 513, 540, 558, 849
Bst4CI	ACNGT	2 cut(s)	328, 943
Bst6I	CTCTTC	1 cut(s)	762
BstACI	GRCGYC	1 cut(s)	77
BstAPI	GCANNNNNTGC	1 cut(s)	827
BstBAI	YACGTR	1 cut(s)	440
BstC8I	GCNNGC	2 cut(s)	7, 50
BstDEI	CTNAG	6 cut(s)	384, 411, 709, 720, 804, 923
BstF5I	GGATG	1 cut(s)	778
BstFNI	CGCG	1 cut(s)	150
BstH2I	RGCGCY	1 cut(s)	94
BstHHI	GCGC	1 cut(s)	93
BstKTI	GATC	8 cut(s)	30, 39, 333, 394, 420, 584, 667, 718
BstMAI	GTCTC	1 cut(s)	957
BstMBI	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
BstMCI	CGRYCG	1 cut(s)	39
BstMWI	GCNNNNNNNGC	4 cut(s)	156, 359, 380, 827
BstNI	CCWGG	6 cut(s)	161, 498, 513, 540, 558, 849
BstSCI	CCNGG	7 cut(s)	120, 159, 496, 511, 538, 556, 847
BstSFI	CTRYAG	1 cut(s)	762
BstUI	CGCG	1 cut(s)	150
BstV1I	GCAGC	3 cut(s)	340, 346, 436
BstV2I	GAAGAC	2 cut(s)	121, 271
BstX2I	RGATCY	3 cut(s)	391, 417, 715
BstYI	RGATCY	3 cut(s)	391, 417, 715
BsuRI	GGCC	2 cut(s)	305, 630
BtsCI	GGATG	1 cut(s)	778
BtsIMutI	CAGTG	3 cut(s)	294, 408, 948
Cac8I	GCNNGC	2 cut(s)	7, 50
CfoI	GCGC	1 cut(s)	93
Cfr13I	GGNCC	2 cut(s)	255, 485
CseI	GACGC	1 cut(s)	139
CsiI	ACCWGGT	1 cut(s)	556
Csp6I	GTAC	4 cut(s)	437, 588, 678, 897
CspCI	CAANNNNNGTGG	2 cut(s)	631, 666
CviAII	CATG	4 cut(s)	428, 697, 866, 976
CviQI	GTAC	4 cut(s)	437, 588, 678, 897
DdeI	CTNAG	6 cut(s)	384, 411, 709, 720, 804, 923
DpnI	GATC	8 cut(s)	29, 38, 332, 393, 419, 583, 666, 717
DpnII	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
DriI	GACNNNNNGTC	1 cut(s)	275
Eam1104I	CTCTTC	1 cut(s)	762
Eam1105I	GACNNNNNGTC	1 cut(s)	275
EarI	CTCTTC	1 cut(s)	762
Eco130I	CCWWGG	1 cut(s)	631
Eco47I	GGWCC	2 cut(s)	255, 485
EcoRII	CCWGG	6 cut(s)	159, 496, 511, 538, 556, 847
EcoT14I	CCWWGG	1 cut(s)	631
ErhI	CCWWGG	1 cut(s)	631
FaeI	CATG	4 cut(s)	431, 700, 869, 979
FalI	AAGNNNNNCTT	2 cut(s)	438, 470
FaqI	GGGAC	3 cut(s)	268, 282, 772
FatI	CATG	4 cut(s)	427, 696, 865, 975
FauI	CCCGC	3 cut(s)	120, 245, 429
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	9 cut(s)	105, 108, 116, 354, 357, 360, 450, 777, 780
FokI	GGATG	1 cut(s)	785
Fsp4HI	GCNGC	9 cut(s)	105, 108, 116, 354, 357, 360, 450, 777, 780
FspBI	CTAG	2 cut(s)	1004, 1012
GlaI	GCGC	1 cut(s)	92
GluI	GCNGC	9 cut(s)	105, 108, 116, 354, 357, 360, 450, 777, 780
GsaI	CCCAGC	1 cut(s)	936
HaeII	RGCGCY	1 cut(s)	94
HaeIII	GGCC	2 cut(s)	305, 630
HapII	CCGG	2 cut(s)	122, 956
HgaI	GACGC	1 cut(s)	139
HhaI	GCGC	1 cut(s)	93
Hin1I	GRCGYC	1 cut(s)	77
Hin1II	CATG	4 cut(s)	431, 700, 869, 979
Hin6I	GCGC	1 cut(s)	91
HinP1I	GCGC	1 cut(s)	91
HincII	GTYRAC	2 cut(s)	72, 136
HindII	GTYRAC	2 cut(s)	72, 136
HindIII	AAGCTT	1 cut(s)	50
HinfI	GANTC	3 cut(s)	165, 919, 950
HpaII	CCGG	2 cut(s)	122, 956
HphI	GGTGA	4 cut(s)	51, 340, 527, 674
Hpy166II	GTNNAC	3 cut(s)	72, 136, 1009
Hpy188I	TCNGA	2 cut(s)	723, 741
Hpy188III	TCNNGA	2 cut(s)	579, 916
Hpy8I	GTNNAC	3 cut(s)	72, 136, 1009
Hpy99I	CGWCG	2 cut(s)	79, 137
HpyAV	CCTTC	3 cut(s)	196, 241, 722
HpyCH4III	ACNGT	2 cut(s)	328, 943
HpyCH4IV	ACGT	3 cut(s)	68, 77, 439
HpyCH4V	TGCA	9 cut(s)	323, 362, 405, 452, 522, 696, 764, 821, 869
HpyF10VI	GCNNNNNNNGC	4 cut(s)	156, 359, 380, 827
HpyF3I	CTNAG	6 cut(s)	384, 411, 709, 720, 804, 923
HpySE526I	ACGT	3 cut(s)	68, 77, 439
Hsp92I	GRCGYC	1 cut(s)	77
Hsp92II	CATG	4 cut(s)	431, 700, 869, 979
HspAI	GCGC	1 cut(s)	91
KpnI	GGTACC	2 cut(s)	591, 900
Kzo9I	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
LmnI	GCTCC	2 cut(s)	847, 872
Lsp1109I	GCAGC	3 cut(s)	340, 346, 436
LweI	GCATC	3 cut(s)	50, 414, 763
MabI	ACCWGGT	1 cut(s)	556
MaeI	CTAG	2 cut(s)	1004, 1012
MaeII	ACGT	3 cut(s)	68, 77, 439
MaeIII	GTNAC	3 cut(s)	95, 235, 937
MalI	GATC	8 cut(s)	29, 38, 332, 393, 419, 583, 666, 717
MboI	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
MboII	GAAGA	3 cut(s)	121, 271, 779
MfeI	CAATTG	1 cut(s)	813
MflI	RGATCY	3 cut(s)	391, 417, 715
MlyI	GAGTC	2 cut(s)	913, 959
MnlI	CCTC	5 cut(s)	351, 406, 729, 763, 990
MroXI	GAANNNNTTC	1 cut(s)	574
MseI	TTAA	1 cut(s)	456
MslI	CAYNNNNRTG	1 cut(s)	221
MspA1I	CMGCKG	1 cut(s)	359
MspI	CCGG	2 cut(s)	122, 956
MspR9I	CCNGG	7 cut(s)	122, 161, 498, 513, 540, 558, 849
MunI	CAATTG	1 cut(s)	813
Mva1269I	GAATGC	1 cut(s)	524
MvaI	CCWGG	6 cut(s)	161, 498, 513, 540, 558, 849
MvnI	CGCG	1 cut(s)	150
MwoI	GCNNNNNNNGC	4 cut(s)	156, 359, 380, 827
NciI	CCSGG	1 cut(s)	122
NdeII	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
NlaIII	CATG	4 cut(s)	431, 700, 869, 979
NlaIV	GGNNCC	5 cut(s)	184, 256, 419, 589, 898
NmeAIII	GCCGAG	1 cut(s)	454
NmuCI	GTSAC	1 cut(s)	95
PcsI	WCGNNNNNNNCGW	1 cut(s)	74
PctI	GAATGC	1 cut(s)	524
PdmI	GAANNNNTTC	1 cut(s)	574
PfeI	GAWTC	1 cut(s)	165
PkrI	GCNGC	9 cut(s)	106, 109, 117, 355, 358, 361, 451, 778, 781
Ple19I	CGATCG	1 cut(s)	39
PleI	GAGTC	2 cut(s)	913, 958
PpsI	GAGTC	2 cut(s)	913, 958
Ppu21I	YACGTR	1 cut(s)	440
Psp6I	CCWGG	6 cut(s)	159, 496, 511, 538, 556, 847
PspFI	CCCAGC	1 cut(s)	932
PspGI	CCWGG	6 cut(s)	159, 496, 511, 538, 556, 847
PspN4I	GGNNCC	5 cut(s)	184, 256, 419, 589, 898
PspPI	GGNCC	2 cut(s)	255, 485
PstI	CTGCAG	1 cut(s)	766
PsuI	RGATCY	3 cut(s)	391, 417, 715
PvuI	CGATCG	1 cut(s)	39
RsaI	GTAC	4 cut(s)	438, 589, 679, 898
RsaNI	GTAC	4 cut(s)	437, 588, 678, 897
RseI	CAYNNNNRTG	1 cut(s)	221
SalI	GTCGAC	1 cut(s)	134
SaqAI	TTAA	1 cut(s)	456
SatI	GCNGC	9 cut(s)	105, 108, 116, 354, 357, 360, 450, 777, 780
Sau3AI	GATC	8 cut(s)	27, 36, 330, 391, 417, 581, 664, 715
Sau96I	GGNCC	2 cut(s)	255, 485
SchI	GAGTC	2 cut(s)	913, 959
ScrFI	CCNGG	7 cut(s)	122, 161, 498, 513, 540, 558, 849
SexAI	ACCWGGT	1 cut(s)	556
SfaNI	GCATC	3 cut(s)	50, 414, 763
SfcI	CTRYAG	1 cut(s)	762
SinI	GGWCC	2 cut(s)	255, 485
SmiMI	CAYNNNNRTG	1 cut(s)	221
SpeI	ACTAGT	1 cut(s)	1003
SspMI	CTAG	2 cut(s)	1004, 1012
StyD4I	CCNGG	7 cut(s)	120, 159, 496, 511, 538, 556, 847
StyI	CCWWGG	1 cut(s)	631
TaaI	ACNGT	2 cut(s)	328, 943
TaiI	ACGT	3 cut(s)	71, 80, 442
TaqI	TCGA	4 cut(s)	35, 135, 207, 580
TaqII	GACCGA	1 cut(s)	272
TauI	GCSGC	6 cut(s)	107, 110, 118, 359, 779, 782
TfiI	GAWTC	1 cut(s)	165
Tru1I	TTAA	1 cut(s)	456
Tru9I	TTAA	1 cut(s)	456
TscAI	CASTG	3 cut(s)	294, 415, 948
TseFI	GTSAC	1 cut(s)	95
TseI	GCWGC	3 cut(s)	353, 359, 449
Tsp45I	GTSAC	1 cut(s)	95
TspDTI	ATGAA	3 cut(s)	45, 539, 615
TspGWI	ACGGA	1 cut(s)	4
TspRI	CASTG	3 cut(s)	294, 415, 948
VpaK11BI	GGWCC	2 cut(s)	255, 485
XapI	RAATTY	1 cut(s)	704
XmiI	GTMKAC	1 cut(s)	135
XmnI	GAANNNNTTC	1 cut(s)	574
XspI	CTAG	2 cut(s)	1004, 1012
ZraI	GACGTC	1 cut(s)	78

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.