AT5G12010
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015028
Physical Location & Seq
Reverse (-)
3877680 .. 3879657
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12010.1

Sequence Viewer

Length: 1509 bp
ATGAAAGCCGCCGTTTTCCGTAACGAAGACGGAGACGAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGTCTGTAATGTTTTCGACGGCGGCGAGGTAGAGTCTAAGAGAAACAACGAAACCAAAAACCTCAAAGGATTTTTCACTTCTTTGCTTTTGATGGAGGAGCACGAGAAGCAAGACCAGGAAGCTCGTAACGCTGCTTCTCGCCGTGAAATGTCTGATTTTCAATCTAATTACCGGAAAAGAGCTAGAACTATGTCCGATTACTACTCCGATCTCAACGATTACTACGCTGATGCTGAGGAAAGTGGAGATATCAATCTGAAGAAGTCACGCGTCTCACGCGCCGTCGCTTCCGTTGCTGTTGCTGCTGCTTCGGAGATTGAGGCGGAGAGCAGTGAGATAACCGGATCCGGATCGGTTCGTGGTACCGGTTCGGGTCAGCAAAGACGGTTATGGGTTAAAGATCGGAGCAGAGCTTGGTGGGAAGAGTGTAGCCGATTGGATTATCCAGAAGAAGATTTCAAAAAAGCGTTTCGAATGTCTAAATCAACGTTTGAGTTAATCTGCGATGAGCTTAATTCAGCGGTTGCTAAAGAAGACACCGCGTTAAGAAACGCGATTCCAGTGCGTCAGCGAGTCGCGGTTTGTATATGGAGATTAGCCACCGGTGAGCCACTTCGTCTTGTCTCCAAGAAGTTTGGTTTAGGAATCTCAACTTGTCACAAGCTTGTTCTTGAGGTATGTAAAGCTATTAAGGACGTTTTAATGCCTAAGTATCTTCAATGGCCAGATGATGAATCTCTTAGAAACATTAGAGAACGTTTCGAATCGGTTTCGGGTATTCCAAACGTTGTTGGCTCTATGTATACTACTCACATTCCGATTATAGCTCCTAAGATTAGTGTAGCCTCTTATTTCAATAAGAGGCATACTGAGAGGAACCAGAAGACGTCGTATTCGATTACAATTCAAGCCGTTGTGAACCCGAAAGGTGTTTTTACTGACTTGTGCATCGGATGGCCCGGGTCAATGCCGGATGATAAGGTGTTAGAGAAGTCATTGTTGTATCAAAGAGCTAACAATGGAGGTTTGTTGAAGGGGATGTGGGTTGCTGGTGGACCGGGTCATCCGTTGTTGGACTGGGTTTTGGTTCCGTATACGCAACAGAACTTGACGTGGACGCAGCATGCGTTTAATGAGAAGATGAGCGAAGTGCAGGGAGTGGCTAAGGAAGCGTTTGGGAGGTTGAAAGGGCGGTGGGCGTGTCTGCAGAAACGGACTGAAGTGAAGCTGCAGGATTTGCCTACCGTTTTGGGTGCTTGTTGTGTGCTTCACAACATATGTGAAATGAGAGAGGAGAAGATGGAGCCAGAGCTGATGGTTGAAGTGATTGATGATGAGGTTTTGCCTGAAAACGTATTGAGATCGGTTAATGCGATGAAGGCTAGAGATACTATCTCACATAATCTATTGCATCACGGCCTCGCGGGTACTTCTTTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

502

Amino Acids

56.81

Weight (kDa)

5.68

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 172 - 252 3.4e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 290 - 447 6.2e-34 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 956
Acc65I GGTACC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 428
AccI GTMKAC 2 cut(s) 869, 1160
AccII CGCG 6 cut(s) 336, 345, 608, 620, 644, 1490
AccIII TCCGGA 1 cut(s) 413
AciI CCGC 8 cut(s) 9, 87, 389, 587, 606, 644, 1258, 1490
AclI AACGTT 3 cut(s) 554, 823, 852
AclWI GGATC 3 cut(s) 405, 418, 424
AcoI YGGCCR 1 cut(s) 788
AcuI CTGAAG 2 cut(s) 344, 1305
AcyI GRCGYC 1 cut(s) 953
AfaI GTAC 2 cut(s) 430, 1495
AflIII ACRYGT 1 cut(s) 334
AgeI ACCGGT 2 cut(s) 431, 668
AgsI TTSAA 8 cut(s) 227, 526, 785, 922, 974, 1099, 1252, 1388
AjiI CACGTC 1 cut(s) 1179
AjnI CCWGG 1 cut(s) 180
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 27, 343, 694
AlwI GGATC 3 cut(s) 405, 418, 424
Ama87I CYCGRG 1 cut(s) 1025
Aor13HI TCCGGA 1 cut(s) 413
AoxI GGCC 3 cut(s) 788, 1022, 1483
ApeKI GCWGC 5 cut(s) 197, 368, 371, 1186, 1294
ArsI GACNNNNNNTTYG 2 cut(s) 943, 975
AsiGI ACCGGT 2 cut(s) 431, 668
Asp718I GGTACC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 1023, 1121
AsuC2I CCSGG 3 cut(s) 1026, 1027, 1125
AsuHPI GGTGA 1 cut(s) 683
AsuII TTCGAA 2 cut(s) 538, 828
AvaI CYCGRG 1 cut(s) 1025
AvaII GGWCC 1 cut(s) 1121
BalI TGGCCA 1 cut(s) 790
BamHI GGATCC 1 cut(s) 410
BanI GGYRCC 1 cut(s) 428
BarI GAAGNNNNNNTAC 2 cut(s) 184, 216
BauI CACGAG 1 cut(s) 167
BbsI GAAGAC 3 cut(s) 33, 606, 956
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 5 cut(s) 184, 355, 358, 1198, 1281
BccI CCATC 4 cut(s) 151, 1014, 1360, 1375
BceAI ACGGC 5 cut(s) 100, 192, 332, 962, 1498
BcgI CGANNNNNNTGC 2 cut(s) 610, 644
BciT130I CCWGG 1 cut(s) 182
BcnI CCSGG 3 cut(s) 1026, 1027, 1125
BcoDI GTCTC 3 cut(s) 27, 343, 694
BfaI CTAG 2 cut(s) 249, 1449
BfmI CTRYAG 2 cut(s) 1271, 1295
BisI GCNGC 7 cut(s) 9, 88, 198, 369, 372, 1187, 1295
BlsI GCNGC 7 cut(s) 10, 89, 199, 370, 373, 1188, 1296
Bme1390I CCNGG 4 cut(s) 182, 1026, 1027, 1125
Bme18I GGWCC 1 cut(s) 1121
BmeT110I CYCGRG 1 cut(s) 1025
BmgBI CACGTC 1 cut(s) 1179
BmgT120I GGNCC 2 cut(s) 1023, 1121
BmiI GGNNCC 5 cut(s) 412, 430, 944, 1155, 1371
BmrFI CCNGG 4 cut(s) 182, 1026, 1027, 1125
BmrI ACTGGG 1 cut(s) 1153
BmsI GCATC 3 cut(s) 286, 1023, 1486
BmuI ACTGGG 1 cut(s) 1153
BpiI GAAGAC 3 cut(s) 33, 606, 956
Bpu10I CCTNAGC 2 cut(s) 300, 1230
Bpu14I TTCGAA 2 cut(s) 538, 828
BpuEI CTTGAG 1 cut(s) 758
BpuMI CCSGG 3 cut(s) 1026, 1027, 1125
BsaBI GATNNNNATC 2 cut(s) 318, 415
BsaHI GRCGYC 1 cut(s) 953
BsaJI CCNNGG 1 cut(s) 1025
BsaWI WCCGGW 5 cut(s) 237, 407, 413, 431, 668
Bse118I RCCGGY 2 cut(s) 431, 668
Bse1I ACTGG 2 cut(s) 626, 1148
Bse8I GATNNNNATC 2 cut(s) 318, 415
BseAI TCCGGA 1 cut(s) 413
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 1 cut(s) 1025
BseGI GGATG 4 cut(s) 1025, 1045, 1110, 1129
BseJI GATNNNNATC 2 cut(s) 318, 415
BseMII CTCAG 2 cut(s) 291, 927
BseNI ACTGG 2 cut(s) 626, 1148
BseRI GAGGAG 4 cut(s) 53, 56, 176, 1373
BseXI GCAGC 5 cut(s) 184, 355, 358, 1198, 1281
BsgI GTGCAG 1 cut(s) 1238
Bsh1236I CGCG 6 cut(s) 336, 345, 608, 620, 644, 1490
BshFI GGCC 3 cut(s) 790, 1024, 1485
BshNI GGYRCC 1 cut(s) 428
BshTI ACCGGT 2 cut(s) 431, 668
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 1025
BsiSI CCGG 8 cut(s) 238, 408, 414, 432, 669, 1026, 1037, 1124
BsmAI GTCTC 3 cut(s) 27, 343, 694
BsmBI CGTCTC 2 cut(s) 27, 343
BsnI GGCC 3 cut(s) 790, 1024, 1485
BsoBI CYCGRG 1 cut(s) 1025
Bsp119I TTCGAA 2 cut(s) 538, 828
Bsp1286I GDGCHC 1 cut(s) 168
Bsp13I TCCGGA 1 cut(s) 413
Bsp143I GATC 5 cut(s) 274, 410, 416, 466, 1427
BspACI CCGC 8 cut(s) 9, 87, 389, 587, 606, 644, 1258, 1490
BspANI GGCC 3 cut(s) 790, 1024, 1485
BspCNI CTCAG 2 cut(s) 292, 928
BspEI TCCGGA 1 cut(s) 413
BspFNI CGCG 6 cut(s) 336, 345, 608, 620, 644, 1490
BspLI GGNNCC 5 cut(s) 412, 430, 944, 1155, 1371
BspMAI CTGCAG 2 cut(s) 1275, 1299
BspPI GGATC 3 cut(s) 405, 418, 424
BspT104I TTCGAA 2 cut(s) 538, 828
BspT107I GGYRCC 1 cut(s) 428
BsrFI RCCGGY 2 cut(s) 431, 668
BsrI ACTGG 2 cut(s) 626, 1148
BssAI RCCGGY 2 cut(s) 431, 668
BssECI CCNNGG 1 cut(s) 1025
BssMI GATC 5 cut(s) 274, 410, 416, 466, 1427
BssNAI GTATAC 2 cut(s) 870, 1161
BssNI GRCGYC 1 cut(s) 953
BssSI CACGAG 1 cut(s) 167
Bst1107I GTATAC 2 cut(s) 870, 1161
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 2 cut(s) 453, 1312
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 1 cut(s) 953
BstAPI GCANNNNNTGC 1 cut(s) 1303
BstBI TTCGAA 2 cut(s) 538, 828
BstC8I GCNNGC 1 cut(s) 1191
BstDEI CTNAG 7 cut(s) 102, 300, 774, 806, 897, 936, 1230
BstF5I GGATG 4 cut(s) 1025, 1045, 1110, 1129
BstFNI CGCG 6 cut(s) 336, 345, 608, 620, 644, 1490
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 5 cut(s) 277, 413, 419, 469, 1430
BstMAI GTCTC 3 cut(s) 27, 343, 694
BstMBI GATC 5 cut(s) 274, 410, 416, 466, 1427
BstMWI GCNNNNNNNGC 8 cut(s) 172, 194, 342, 359, 368, 1235, 1303, 1445
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 1193
BstSCI CCNGG 4 cut(s) 180, 1024, 1025, 1123
BstSFI CTRYAG 2 cut(s) 1271, 1295
BstUI CGCG 6 cut(s) 336, 345, 608, 620, 644, 1490
BstV1I GCAGC 5 cut(s) 184, 355, 358, 1198, 1281
BstV2I GAAGAC 3 cut(s) 33, 606, 956
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
BstZ17I GTATAC 2 cut(s) 870, 1161
BsuRI GGCC 3 cut(s) 790, 1024, 1485
BtgZI GCGATG 2 cut(s) 585, 1454
BtrI CACGTC 1 cut(s) 1179
BtsCI GGATG 4 cut(s) 1025, 1045, 1110, 1129
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 2 cut(s) 403, 633
Cac8I GCNNGC 1 cut(s) 1191
CfoI GCGC 1 cut(s) 347
Cfr10I RCCGGY 2 cut(s) 431, 668
Cfr13I GGNCC 2 cut(s) 1023, 1121
Cfr9I CCCGGG 1 cut(s) 1025
CseI GACGC 3 cut(s) 325, 620, 1192
Csp6I GTAC 2 cut(s) 429, 1494
CspAI ACCGGT 2 cut(s) 431, 668
CviAII CATG 1 cut(s) 1190
CviQI GTAC 2 cut(s) 429, 1494
DdeI CTNAG 7 cut(s) 102, 300, 774, 806, 897, 936, 1230
DpnI GATC 5 cut(s) 276, 412, 418, 468, 1429
DpnII GATC 5 cut(s) 274, 410, 416, 466, 1427
EaeI YGGCCR 1 cut(s) 788
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EciI GGCGGA 1 cut(s) 404
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 1121
Eco57I CTGAAG 2 cut(s) 344, 1305
Eco88I CYCGRG 1 cut(s) 1025
EcoRII CCWGG 1 cut(s) 180
EcoRV GATATC 1 cut(s) 316
Esp3I CGTCTC 2 cut(s) 27, 343
FaeI CATG 1 cut(s) 1193
FatI CATG 1 cut(s) 1189
FauI CCCGC 1 cut(s) 1483
FauNDI CATATG 1 cut(s) 1343
FblI GTMKAC 2 cut(s) 869, 1160
Fnu4HI GCNGC 7 cut(s) 9, 88, 198, 369, 372, 1187, 1295
FokI GGATG 4 cut(s) 1032, 1052, 1116, 1117
Fsp4HI GCNGC 7 cut(s) 9, 88, 198, 369, 372, 1187, 1295
FspBI CTAG 2 cut(s) 249, 1449
GlaI GCGC 1 cut(s) 346
GluI GCNGC 7 cut(s) 9, 88, 198, 369, 372, 1187, 1295
HaeIII GGCC 3 cut(s) 790, 1024, 1485
HapII CCGG 8 cut(s) 238, 408, 414, 432, 669, 1026, 1037, 1124
HgaI GACGC 3 cut(s) 325, 620, 1192
HhaI GCGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 953
Hin1II CATG 1 cut(s) 1193
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 6 cut(s) 98, 622, 639, 711, 800, 830
HpaII CCGG 8 cut(s) 238, 408, 414, 432, 669, 1026, 1037, 1124
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 5 cut(s) 870, 985, 1121, 1161, 1182
Hpy188I TCNGA 8 cut(s) 220, 262, 274, 324, 379, 471, 885, 1019
Hpy188III TCNNGA 3 cut(s) 414, 512, 737
Hpy8I GTNNAC 5 cut(s) 870, 985, 1121, 1161, 1182
Hpy99I CGWCG 3 cut(s) 86, 353, 958
HpyAV CCTTC 2 cut(s) 1093, 1438
HpyCH4III ACNGT 2 cut(s) 453, 1312
HpyCH4IV ACGT 7 cut(s) 554, 762, 823, 852, 953, 1178, 1419
HpyCH4V TGCA 5 cut(s) 1014, 1219, 1273, 1297, 1477
HpyF10VI GCNNNNNNNGC 8 cut(s) 172, 194, 342, 359, 368, 1235, 1303, 1445
HpyF3I CTNAG 7 cut(s) 102, 300, 774, 806, 897, 936, 1230
HpySE526I ACGT 7 cut(s) 554, 762, 823, 852, 953, 1178, 1419
Hsp92I GRCGYC 1 cut(s) 953
Hsp92II CATG 1 cut(s) 1193
HspAI GCGC 1 cut(s) 345
Kpn2I TCCGGA 1 cut(s) 413
KpnI GGTACC 1 cut(s) 432
Kzo9I GATC 5 cut(s) 274, 410, 416, 466, 1427
LmnI GCTCC 4 cut(s) 163, 471, 898, 1369
Lsp1109I GCAGC 5 cut(s) 184, 355, 358, 1198, 1281
LweI GCATC 3 cut(s) 286, 1023, 1486
MaeI CTAG 2 cut(s) 249, 1449
MaeII ACGT 7 cut(s) 554, 762, 823, 852, 953, 1178, 1419
MaeIII GTNAC 4 cut(s) 20, 191, 330, 722
MalI GATC 5 cut(s) 276, 412, 418, 468, 1429
MboI GATC 5 cut(s) 274, 410, 416, 466, 1427
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 790
MluCI AATT 3 cut(s) 232, 580, 969
MluI ACGCGT 1 cut(s) 334
MluNI TGGCCA 1 cut(s) 790
MlyI GAGTC 2 cut(s) 107, 648
MmeI TCCRAC 1 cut(s) 1119
Mox20I TGGCCA 1 cut(s) 790
MroI TCCGGA 1 cut(s) 413
MscI TGGCCA 1 cut(s) 790
MseI TTAA 8 cut(s) 462, 563, 579, 611, 756, 767, 1197, 1434
Msp20I TGGCCA 1 cut(s) 790
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 8 cut(s) 238, 408, 414, 432, 669, 1026, 1037, 1124
MspR9I CCNGG 4 cut(s) 182, 1026, 1027, 1125
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 6 cut(s) 336, 345, 608, 620, 644, 1490
MwoI GCNNNNNNNGC 8 cut(s) 172, 194, 342, 359, 368, 1235, 1303, 1445
NciI CCSGG 3 cut(s) 1026, 1027, 1125
NdeI CATATG 1 cut(s) 1343
NdeII GATC 5 cut(s) 274, 410, 416, 466, 1427
NlaIII CATG 1 cut(s) 1193
NlaIV GGNNCC 5 cut(s) 412, 430, 944, 1155, 1371
NmuCI GTSAC 2 cut(s) 330, 722
NspI RCATGY 1 cut(s) 1193
NspV TTCGAA 2 cut(s) 538, 828
PaeI GCATGC 1 cut(s) 1193
PcsI WCGNNNNNNNCGW 4 cut(s) 87, 959, 1190, 1436
PfeI GAWTC 4 cut(s) 622, 711, 800, 830
PflFI GACNNNGTC 1 cut(s) 1125
PinAI ACCGGT 2 cut(s) 431, 668
PkrI GCNGC 7 cut(s) 10, 89, 199, 370, 373, 1188, 1296
PleI GAGTC 2 cut(s) 106, 647
PpsI GAGTC 2 cut(s) 106, 647
Psp1406I AACGTT 3 cut(s) 554, 823, 852
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 5 cut(s) 412, 430, 944, 1155, 1371
PspPI GGNCC 2 cut(s) 1023, 1121
PstI CTGCAG 2 cut(s) 1275, 1299
PsuI RGATCY 1 cut(s) 410
PsyI GACNNNGTC 1 cut(s) 1125
RsaI GTAC 2 cut(s) 430, 1495
RsaNI GTAC 2 cut(s) 429, 1494
SaqAI TTAA 8 cut(s) 462, 563, 579, 611, 756, 767, 1197, 1434
SatI GCNGC 7 cut(s) 9, 88, 198, 369, 372, 1187, 1295
Sau3AI GATC 5 cut(s) 274, 410, 416, 466, 1427
Sau96I GGNCC 2 cut(s) 1023, 1121
SchI GAGTC 2 cut(s) 107, 648
ScrFI CCNGG 4 cut(s) 182, 1026, 1027, 1125
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 3 cut(s) 286, 1023, 1486
SfcI CTRYAG 2 cut(s) 1271, 1295
SfuI TTCGAA 2 cut(s) 538, 828
SgrAI CRCCGGYG 1 cut(s) 668
SinI GGWCC 1 cut(s) 1121
SmaI CCCGGG 1 cut(s) 1027
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
SphI GCATGC 1 cut(s) 1193
Sse9I AATT 3 cut(s) 232, 580, 969
SsiI CCGC 8 cut(s) 9, 87, 389, 587, 606, 644, 1258, 1490
SspMI CTAG 2 cut(s) 249, 1449
StyD4I CCNGG 4 cut(s) 180, 1024, 1025, 1123
TaaI ACNGT 2 cut(s) 453, 1312
TaiI ACGT 7 cut(s) 557, 765, 826, 855, 956, 1181, 1422
TaqI TCGA 4 cut(s) 81, 538, 828, 962
TasI AATT 3 cut(s) 232, 580, 969
TauI GCSGC 2 cut(s) 11, 90
TfiI GAWTC 4 cut(s) 622, 711, 800, 830
Tru1I TTAA 8 cut(s) 462, 563, 579, 611, 756, 767, 1197, 1434
Tru9I TTAA 8 cut(s) 462, 563, 579, 611, 756, 767, 1197, 1434
TscAI CASTG 2 cut(s) 403, 633
TseFI GTSAC 2 cut(s) 330, 722
TseI GCWGC 5 cut(s) 197, 368, 371, 1186, 1294
Tsp45I GTSAC 2 cut(s) 330, 722
TspDTI ATGAA 3 cut(s) 17, 813, 1457
TspGWI ACGGA 6 cut(s) 8, 45, 346, 1122, 1146, 1294
TspMI CCCGGG 1 cut(s) 1025
TspRI CASTG 2 cut(s) 403, 633
Tth111I GACNNNGTC 1 cut(s) 1125
VpaK11BI GGWCC 1 cut(s) 1121
XceI RCATGY 1 cut(s) 1193
XmaI CCCGGG 1 cut(s) 1025
XmiI GTMKAC 2 cut(s) 869, 1160
XspI CTAG 2 cut(s) 249, 1449
ZraI GACGTC 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.