MD15G1055200.v1.1
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015028
Physical Location & Seq
Reverse (-)
3737253 .. 3737790
538 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1055200.v1.1.491

Sequence Viewer

Length: 474 bp
ATGCCGGATGATCAGGTGCTGGAGAAATCGGCGTTAAATCAGAGGGCTAATAACGGTTTGCTGAAGAACGTGTGGATTGTTGGGGGTGCTGGGTACCCTTTGATGGACTGGGTGCTGGTGCCTTACACCCAGCAGAACCTAACGTGGACCCAGCACGCTTTCAACGAGAAGATTGGGGAGATTCAGAATGTGGCCAGAGATGCGTTTGCTAGGTTGAAGGGAAGGTGGTTTTGCTTGCAGAAGAGGACGGAGGTGAAGCTGCAGGACTTGCCGGTGGTTCTCGGGGCGTGCTGTGTTTTGCACAACATTTGTGAGTTGAGGAATGAGGAGATGGAGCCCGAGCTCCGGTTCGAGCTCATCGACGACGAGATGGTCCCGGAGGTCGCTCTTAGGTCGACGAGCTCGATGAAGGCTAGGGATGCCATTGCCCATAATCTATTGCACCATGGCCTGGCGGGCACTAGTTTCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

158

Amino Acids

17.83

Weight (kDa)

5.71

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 2 - 102 2e-20 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 371
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 93, 118
AccB7I CCANNNNNTGG 1 cut(s) 451
AccI GTMKAC 1 cut(s) 395
AciI CCGC 1 cut(s) 455
AcoI YGGCCR 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 3 cut(s) 103, 345, 451
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 2 cut(s) 163, 217
AhlI ACTAGT 1 cut(s) 461
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 4 cut(s) 259, 343, 355, 402
AluI AGCT 4 cut(s) 259, 343, 355, 402
Alw21I GWGCWC 3 cut(s) 345, 357, 404
AlwNI CAGNNNCTG 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 281, 338
AoxI GGCC 2 cut(s) 192, 448
ApeKI GCWGC 1 cut(s) 259
Asp718I GGTACC 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 147, 373
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 2 cut(s) 281, 338
AvaII GGWCC 2 cut(s) 147, 373
BaeGI GKGCMC 1 cut(s) 461
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 2 cut(s) 93, 118
BanII GRGCYC 4 cut(s) 339, 345, 357, 404
Bbv12I GWGCWC 3 cut(s) 345, 357, 404
BbvI GCAGC 1 cut(s) 246
BccI CCATC 3 cut(s) 97, 325, 364
BciT130I CCWGG 1 cut(s) 452
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 1 cut(s) 377
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 3 cut(s) 210, 414, 462
BfmI CTRYAG 1 cut(s) 260
BglI GCCNNNNNGGC 1 cut(s) 456
BisI GCNGC 1 cut(s) 260
BlsI GCNGC 1 cut(s) 261
Bme1390I CCNGG 2 cut(s) 377, 452
Bme18I GGWCC 2 cut(s) 147, 373
BmeT110I CYCGRG 2 cut(s) 281, 338
BmgT120I GGNCC 2 cut(s) 147, 373
BmiI GGNNCC 5 cut(s) 95, 120, 149, 336, 375
BmrFI CCNGG 2 cut(s) 377, 452
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 190, 409
BmuI ACTGGG 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 345
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 345, 451
Bse118I RCCGGY 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 113
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 2 cut(s) 13, 424
BseLI CCNNNNNNNGG 3 cut(s) 103, 345, 451
BseMI GCAATG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 341
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 1 cut(s) 246
BseYI CCCAGC 3 cut(s) 89, 129, 150
BshFI GGCC 2 cut(s) 194, 450
BshNI GGYRCC 2 cut(s) 93, 118
BsiHKAI GWGCWC 3 cut(s) 345, 357, 404
BsiHKCI CYCGRG 2 cut(s) 281, 338
BsiSI CCGG 4 cut(s) 5, 272, 346, 377
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 103, 345, 451
BsmFI GGGAC 1 cut(s) 359
BsnI GGCC 2 cut(s) 194, 450
BsoBI CYCGRG 2 cut(s) 281, 338
Bsp1286I GDGCHC 5 cut(s) 339, 345, 357, 404, 461
Bsp143I GATC 1 cut(s) 10
Bsp19I CCATGG 1 cut(s) 445
BspACI CCGC 1 cut(s) 455
BspANI GGCC 2 cut(s) 194, 450
BspLI GGNNCC 5 cut(s) 95, 120, 149, 336, 375
BspMAI CTGCAG 1 cut(s) 264
BspT107I GGYRCC 2 cut(s) 93, 118
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 271
BsrI ACTGG 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 452
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 268
BstC8I GCNNGC 4 cut(s) 156, 236, 289, 457
BstDEI CTNAG 1 cut(s) 389
BstDSI CCRYGG 1 cut(s) 445
BstF5I GGATG 2 cut(s) 13, 424
BstKTI GATC 1 cut(s) 13
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 4 cut(s) 200, 268, 419, 456
BstNI CCWGG 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 375, 450
BstSFI CTRYAG 1 cut(s) 260
BstSLI GKGCMC 1 cut(s) 461
BstV1I GCAGC 1 cut(s) 246
BsuRI GGCC 2 cut(s) 194, 450
BtgI CCRYGG 1 cut(s) 445
BtsCI GGATG 2 cut(s) 13, 424
Cac8I GCNNGC 4 cut(s) 156, 236, 289, 457
CaiI CAGNNNCTG 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 147, 373
Csp6I GTAC 1 cut(s) 94
CviAII CATG 1 cut(s) 446
CviJI RGCY 9 cut(s) 47, 194, 259, 337, 343, 355, 402, 413, 450
CviKI_1 RGCY 9 cut(s) 47, 194, 259, 337, 343, 355, 402, 413, 450
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 389
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 3 cut(s) 343, 355, 402
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 4 cut(s) 339, 345, 357, 404
Eco47I GGWCC 2 cut(s) 147, 373
Eco53kI GAGCTC 3 cut(s) 343, 355, 402
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 2 cut(s) 281, 338
EcoICRI GAGCTC 3 cut(s) 343, 355, 402
EcoRII CCWGG 1 cut(s) 450
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 4 cut(s) 339, 345, 357, 404
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 1 cut(s) 449
FaiI YATR 2 cut(s) 432, 447
FaqI GGGAC 1 cut(s) 359
FatI CATG 1 cut(s) 445
FauI CCCGC 1 cut(s) 448
FbaI TGATCA 1 cut(s) 10
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 1 cut(s) 260
FokI GGATG 2 cut(s) 20, 431
FriOI GRGCYC 4 cut(s) 339, 345, 357, 404
Fsp4HI GCNGC 1 cut(s) 260
FspBI CTAG 3 cut(s) 210, 414, 462
GluI GCNGC 1 cut(s) 260
GsaI CCCAGC 3 cut(s) 93, 133, 154
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 2 cut(s) 194, 450
HapII CCGG 4 cut(s) 5, 272, 346, 377
Hin1II CATG 1 cut(s) 449
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 1 cut(s) 181
HpaII CCGG 4 cut(s) 5, 272, 346, 377
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 2 cut(s) 147, 396
Hpy188I TCNGA 2 cut(s) 42, 186
Hpy8I GTNNAC 2 cut(s) 147, 396
Hpy99I CGWCG 3 cut(s) 365, 368, 400
HpyAV CCTTC 3 cut(s) 211, 216, 403
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4IV ACGT 2 cut(s) 69, 143
HpyCH4V TGCA 4 cut(s) 238, 262, 301, 442
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 268, 419, 456
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 2 cut(s) 69, 143
Hsp92II CATG 1 cut(s) 449
KpnI GGTACC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 1 cut(s) 10
LmnI GCTCC 2 cut(s) 334, 348
Lsp1109I GCAGC 1 cut(s) 246
LweI GCATC 2 cut(s) 190, 409
MaeI CTAG 3 cut(s) 210, 414, 462
MaeII ACGT 2 cut(s) 69, 143
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MboII GAAGA 3 cut(s) 76, 181, 253
MhlI GDGCHC 5 cut(s) 339, 345, 357, 404, 461
MlsI TGGCCA 1 cut(s) 194
MluNI TGGCCA 1 cut(s) 194
MnlI CCTC 6 cut(s) 36, 237, 244, 312, 319, 373
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
MseI TTAA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 194
MspI CCGG 4 cut(s) 5, 272, 346, 377
MspR9I CCNGG 2 cut(s) 377, 452
MvaI CCWGG 1 cut(s) 452
MwoI GCNNNNNNNGC 4 cut(s) 200, 268, 419, 456
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 445
NdeII GATC 1 cut(s) 10
NlaIII CATG 1 cut(s) 449
NlaIV GGNNCC 5 cut(s) 95, 120, 149, 336, 375
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 357, 401
PfeI GAWTC 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 451
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 1 cut(s) 261
Psp124BI GAGCTC 3 cut(s) 345, 357, 404
Psp6I CCWGG 1 cut(s) 450
PspFI CCCAGC 3 cut(s) 89, 129, 150
PspGI CCWGG 1 cut(s) 450
PspN4I GGNNCC 5 cut(s) 95, 120, 149, 336, 375
PspPI GGNCC 2 cut(s) 147, 373
PstI CTGCAG 1 cut(s) 264
PstNI CAGNNNCTG 1 cut(s) 19
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SacI GAGCTC 3 cut(s) 345, 357, 404
SalI GTCGAC 1 cut(s) 394
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 260
Sau3AI GATC 1 cut(s) 10
Sau96I GGNCC 2 cut(s) 147, 373
ScrFI CCNGG 2 cut(s) 377, 452
SduI GDGCHC 5 cut(s) 339, 345, 357, 404, 461
SfaNI GCATC 2 cut(s) 190, 409
SfcI CTRYAG 1 cut(s) 260
SinI GGWCC 2 cut(s) 147, 373
SpeI ACTAGT 1 cut(s) 461
SsiI CCGC 1 cut(s) 455
SspMI CTAG 3 cut(s) 210, 414, 462
SstI GAGCTC 3 cut(s) 345, 357, 404
StyD4I CCNGG 2 cut(s) 375, 450
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 1 cut(s) 56
TaiI ACGT 2 cut(s) 72, 146
TaqI TCGA 4 cut(s) 351, 360, 395, 404
TfiI GAWTC 1 cut(s) 181
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseI GCWGC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 422
TspGWI ACGGA 1 cut(s) 263
Van91I CCANNNNNTGG 1 cut(s) 451
VpaK11BI GGWCC 2 cut(s) 147, 373
XmiI GTMKAC 1 cut(s) 395
XspI CTAG 3 cut(s) 210, 414, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.