RchiOBHm_Chr6g0311321
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015028
Physical Location & Seq
Forward (+)
68307912 .. 68309970
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27997

Sequence Viewer

Length: 1536 bp
ATGAAATTGAAACCCATACCCAACCCGGACCCAGACCTAGACCAACCCGACCTCTCCTACATCTACTCCTTCCTCCACTCCTCTCTCCACCACCAAATGAACAATGATACCGCCAAGAAACGCCGTCGTAAGACCGCCGACGACGACGGCCCGCCGGGAAAGCAAGGCAAGATGAAGGACCTGAAGGGGATCATCACCACCCTGTCGCTGCTGGAGCAGCAGGAGAAGGAGGACCTCGACCGGGCCTCAAATGACGACAAGTCGTTTGCGGAAGAGAAGCAGAAGCAGACGACGAAGACCAAGGCCATGGTGGAACACTACTCCGATTTGCAGGGCTACTACTCGGACGTCGAGGAGTCCGAGACCGTGAACCGCAAGAAGTCGAGAAACGCGGCGACTGCGGCCGTTGCGGCGGCGGCGGCGGTTGACGCCTCCGAAAAGGGCGCGAAACCCGGAGGAAGCGGGTCGGGTCACCAGCGGAGGCTTTGGGTCAAGGACCGGTCCAAGGCCTGGTGGGACGAGTGTAACCAACCCGATTTCCCGGAAGAGGAATTCAAAAAAGCCTTCCGGATGGGGAAGGCCACGTTCGATTTGATCTGCGACGAGCTCAATTCGGCTATAGCGAAAGAGGACACCACTCTCCGGAATGCAATTCCGGTCCGGCAGAGAGTCGCCGTCTGTTTGTGGAGGTTAGCAACCGGCGACCCGCTCCGGCTCGTTTCAAAGCGATTCGGGTTAGGGATATCGACTTGTCATAAACTAGTACTCGAAGTCTGCTCAGCTATTAGGACTGTGTTGATGCCCAAGTACTTGAATTGGCCGGAGGATGGTGTTTTGAGGAAGGTGAAGGAAGAGTTCGAGTCGTTTTCAGGTATACCTAATGTTGTAGGGTCTATGTACACTACTCATATTCCGATCATAGCTCCGAAGATTAGTGTGGCTGCTTATTTTAACAAGAGGCATACAGAGAGGAACCAGAAGACTAGCTACTCCATCACCGTGCAAGGTGTGGTGAATCCGAAAGGGGTGTTCACTGATGTGTGTATAGGGTGGCCTGGTTCAATGCCGGATGATCAGGTTCTGGAGAAGTCGGCTTTACATCAGAGGGCCGGGAATGGTTTGCTTAAAGGTGTGTGGATTGTGGGGGGTTCGGGGTACCCTTTGCTGGATTGGGTGCTGGTGCCGTATACTCAGCAGCATCTTACTTGGACGCAGCATGCGTTCAATGAGAAGATTGGGGAGATTCAGAATGTGTCGAAAGATGCTTTTGCGAGGTTGAAGGGGAGGTGGTTGTGCTTGCAGAAGAGGACGGAGGTGAAGCTCCAGGACTTGCCTGTGGTTCTTGGGGCTTGCTGTGTGCTGCACAATATATGCGAGCTGAGGAATGAGGAAATCGACCCCGACCTGAGGTTTGAGCTTGTGGATGATGAGATGGTTCCGGAGATTGCTGTGAGATCACAAAGCTCGAAGAAGGCGAGGGATGCCATTGCTCATAATCTTTTGCATCATGGCCTTGCAGGCACTTCTTTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

511

Amino Acids

57.31

Weight (kDa)

8.36

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 182 - 262 8.2e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 300 - 456 1.1e-34 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 1155
AccB1I GGYRCC 2 cut(s) 1155, 1180
AccB7I CCANNNNNTGG 1 cut(s) 510
AccBSI CCGCTC 1 cut(s) 709
AccI GTMKAC 2 cut(s) 874, 1187
AccII CGCG 2 cut(s) 392, 446
AccIII TCCGGA 3 cut(s) 567, 642, 1438
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 2 cut(s) 402, 818
AcsI RAATTY 1 cut(s) 551
AcuI CTGAAG 1 cut(s) 203
AcyI GRCGYC 2 cut(s) 348, 429
AfaI GTAC 4 cut(s) 765, 809, 899, 1157
AfiI CCNNNNNNNGG 8 cut(s) 241, 505, 510, 547, 642, 827, 1165, 1407
AgeI ACCGGT 1 cut(s) 498
AgsI TTSAA 7 cut(s) 10, 556, 723, 814, 1062, 1225, 1279
AhdI GACNNNNNGTC 1 cut(s) 259
AhlI ACTAGT 1 cut(s) 760
AjnI CCWGG 3 cut(s) 509, 1054, 1323
AleI CACNNNNGTG 1 cut(s) 1037
AluBI AGCT 8 cut(s) 607, 782, 923, 987, 1321, 1378, 1417, 1464
AluI AGCT 8 cut(s) 607, 782, 923, 987, 1321, 1378, 1417, 1464
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 1081
Aor13HI TCCGGA 3 cut(s) 567, 642, 1438
ApeKI GCWGC 6 cut(s) 208, 217, 941, 1195, 1213, 1360
ApoI RAATTY 1 cut(s) 551
ArsI GACNNNNNNTTYG 4 cut(s) 248, 280, 1394, 1426
AsiGI ACCGGT 1 cut(s) 498
Asp718I GGTACC 1 cut(s) 1155
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 496, 501, 658, 1107
AsuC2I CCSGG 6 cut(s) 26, 156, 242, 453, 542, 1111
AsuHPI GGTGA 6 cut(s) 187, 464, 856, 988, 1024, 1327
AvaII GGWCC 6 cut(s) 28, 178, 232, 496, 501, 658
AxyI CCTNAGG 1 cut(s) 1406
BanI GGYRCC 2 cut(s) 1155, 1180
BanII GRGCYC 1 cut(s) 609
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BbsI GAAGAC 2 cut(s) 302, 986
Bbv12I GWGCWC 1 cut(s) 609
BbvCI CCTCAGC 1 cut(s) 1379
BbvI GCAGC 6 cut(s) 195, 229, 928, 1207, 1225, 1347
BccI CCATC 4 cut(s) 565, 821, 1001, 1426
BceAI ACGGC 5 cut(s) 108, 163, 389, 659, 1168
BciT130I CCWGG 3 cut(s) 511, 1056, 1325
BclI TGATCA 1 cut(s) 1072
BcnI CCSGG 6 cut(s) 26, 156, 242, 453, 542, 1111
BcoDI GTCTC 1 cut(s) 356
BcuI ACTAGT 1 cut(s) 760
BfaI CTAG 4 cut(s) 38, 761, 984, 1534
BfmI CTRYAG 1 cut(s) 618
BglI GCCNNNNNGGC 2 cut(s) 410, 1518
BlpI GCTNAGC 1 cut(s) 778
BmcAI AGTACT 2 cut(s) 765, 809
Bme1390I CCNGG 9 cut(s) 26, 156, 242, 453, 511, 542, 1056, 1111, 1325
Bme18I GGWCC 6 cut(s) 28, 178, 232, 496, 501, 658
BmeRI GACNNNNNGTC 1 cut(s) 259
BmgT120I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 496, 501, 658, 1107
BmiI GGNNCC 5 cut(s) 30, 974, 1157, 1182, 1437
BmrFI CCNGG 9 cut(s) 26, 156, 242, 453, 511, 542, 1056, 1111, 1325
BmsI GCATC 5 cut(s) 789, 1207, 1252, 1471, 1513
BpiI GAAGAC 2 cut(s) 302, 986
BpmI CTGGAG 3 cut(s) 233, 1103, 1307
Bpu10I CCTNAGC 1 cut(s) 1379
Bpu1102I GCTNAGC 1 cut(s) 778
BpuMI CCSGG 6 cut(s) 26, 156, 242, 453, 542, 1111
BsaHI GRCGYC 2 cut(s) 348, 429
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 3 cut(s) 300, 306, 504
BsaWI WCCGGW 5 cut(s) 498, 567, 642, 655, 1438
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 8 cut(s) 241, 505, 510, 547, 642, 827, 1165, 1407
Bse118I RCCGGY 2 cut(s) 498, 698
Bse21I CCTNAGG 1 cut(s) 1406
Bse3DI GCAATG 1 cut(s) 1485
BseAI TCCGGA 3 cut(s) 567, 642, 1438
BseBI CCWGG 3 cut(s) 511, 1056, 1325
BseDI CCNNGG 3 cut(s) 300, 306, 504
BseGI GGATG 5 cut(s) 576, 832, 1075, 1429, 1486
BseLI CCNNNNNNNGG 8 cut(s) 241, 505, 510, 547, 642, 827, 1165, 1407
BseMI GCAATG 1 cut(s) 1485
BseMII CTCAG 4 cut(s) 792, 1205, 1370, 1397
BseRI GAGGAG 2 cut(s) 70, 368
BseX3I CGGCCG 1 cut(s) 402
BseXI GCAGC 6 cut(s) 195, 229, 928, 1207, 1225, 1347
BsgI GTGCAG 1 cut(s) 1346
Bsh1236I CGCG 2 cut(s) 392, 446
Bsh1285I CGRYCG 2 cut(s) 241, 405
BshNI GGYRCC 2 cut(s) 1155, 1180
BshTI ACCGGT 1 cut(s) 498
BsiEI CGRYCG 2 cut(s) 241, 405
BsiHKAI GWGCWC 1 cut(s) 609
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 8 cut(s) 241, 505, 510, 547, 642, 827, 1165, 1407
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 530
BsmI GAATGC 1 cut(s) 652
Bso31I GGTCTC 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 609
Bsp13I TCCGGA 3 cut(s) 567, 642, 1438
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 5 cut(s) 189, 594, 915, 1072, 1454
Bsp1720I GCTNAGC 1 cut(s) 778
Bsp19I CCATGG 1 cut(s) 306
BspCNI CTCAG 4 cut(s) 791, 1204, 1371, 1398
BspEI TCCGGA 3 cut(s) 567, 642, 1438
BspFNI CGCG 2 cut(s) 392, 446
BspLI GGNNCC 5 cut(s) 30, 974, 1157, 1182, 1437
BspPI GGATC 1 cut(s) 197
BspT107I GGYRCC 2 cut(s) 1155, 1180
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 709
BsrDI GCAATG 1 cut(s) 1485
BsrFI RCCGGY 2 cut(s) 498, 698
BsrGI TGTACA 1 cut(s) 897
BssAI RCCGGY 2 cut(s) 498, 698
BssECI CCNNGG 3 cut(s) 300, 306, 504
BssMI GATC 5 cut(s) 189, 594, 915, 1072, 1454
BssNAI GTATAC 2 cut(s) 875, 1188
BssNI GRCGYC 2 cut(s) 348, 429
BssT1I CCWWGG 3 cut(s) 300, 306, 504
Bst1107I GTATAC 2 cut(s) 875, 1188
Bst2UI CCWGG 3 cut(s) 511, 1056, 1325
Bst4CI ACNGT 3 cut(s) 367, 793, 1000
Bst6I CTCTTC 4 cut(s) 267, 540, 846, 1298
BstACI GRCGYC 2 cut(s) 348, 429
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 6 cut(s) 152, 1218, 1298, 1351, 1376, 1519
BstDEI CTNAG 4 cut(s) 778, 1191, 1379, 1406
BstDSI CCRYGG 1 cut(s) 306
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 5 cut(s) 576, 832, 1075, 1429, 1486
BstFNI CGCG 2 cut(s) 392, 446
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 5 cut(s) 192, 597, 918, 1075, 1457
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 5 cut(s) 189, 594, 915, 1072, 1454
BstMCI CGRYCG 2 cut(s) 241, 405
BstNI CCWGG 3 cut(s) 511, 1056, 1325
BstNSI RCATGY 1 cut(s) 1220
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 9 cut(s) 24, 154, 240, 451, 509, 540, 1054, 1109, 1323
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 2 cut(s) 392, 446
BstV1I GCAGC 6 cut(s) 195, 229, 928, 1207, 1225, 1347
BstV2I GAAGAC 2 cut(s) 302, 986
BstXI CCANNNNNNTGG 1 cut(s) 307
BstZ17I GTATAC 2 cut(s) 875, 1188
BstZI CGGCCG 1 cut(s) 402
Bsu36I CCTNAGG 1 cut(s) 1406
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 5 cut(s) 576, 832, 1075, 1429, 1486
BtsIMutI CAGTG 1 cut(s) 1032
Cac8I GCNNGC 6 cut(s) 152, 1218, 1298, 1351, 1376, 1519
CaiI CAGNNNCTG 1 cut(s) 1081
CfoI GCGC 1 cut(s) 446
Cfr10I RCCGGY 2 cut(s) 498, 698
Cfr13I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 496, 501, 658, 1107
CpoI CGGWCCG 1 cut(s) 658
CseI GACGC 2 cut(s) 437, 1219
Csp6I GTAC 4 cut(s) 764, 808, 898, 1156
CspAI ACCGGT 1 cut(s) 498
CspI CGGWCCG 1 cut(s) 658
CviAII CATG 3 cut(s) 307, 1217, 1508
CviQI GTAC 4 cut(s) 764, 808, 898, 1156
DdeI CTNAG 4 cut(s) 778, 1191, 1379, 1406
DpnI GATC 5 cut(s) 191, 596, 917, 1074, 1456
DpnII GATC 5 cut(s) 189, 594, 915, 1072, 1454
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 2 cut(s) 402, 818
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 4 cut(s) 267, 540, 846, 1298
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 4 cut(s) 267, 540, 846, 1298
Ecl136II GAGCTC 1 cut(s) 607
EclXI CGGCCG 1 cut(s) 402
Eco130I CCWWGG 3 cut(s) 300, 306, 504
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 609
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 744
Eco47I GGWCC 6 cut(s) 28, 178, 232, 496, 501, 658
Eco52I CGGCCG 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 203
Eco81I CCTNAGG 1 cut(s) 1406
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 607
EcoO109I RGGNCCY 2 cut(s) 178, 232
EcoO65I GGTNACC 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 3 cut(s) 509, 1054, 1323
EcoRV GATATC 1 cut(s) 744
EcoT14I CCWWGG 3 cut(s) 300, 306, 504
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 3 cut(s) 300, 306, 504
FaeI CATG 3 cut(s) 310, 1220, 1511
FaqI GGGAC 1 cut(s) 530
FatI CATG 3 cut(s) 306, 1216, 1507
FauI CCCGC 3 cut(s) 159, 455, 714
FbaI TGATCA 1 cut(s) 1072
FblI GTMKAC 2 cut(s) 874, 1187
FokI GGATG 5 cut(s) 583, 839, 1082, 1436, 1493
FriOI GRGCYC 1 cut(s) 609
FspBI CTAG 4 cut(s) 38, 761, 984, 1534
GlaI GCGC 1 cut(s) 445
GsuI CTGGAG 3 cut(s) 233, 1103, 1307
HgaI GACGC 2 cut(s) 437, 1219
HhaI GCGC 1 cut(s) 446
Hin1I GRCGYC 2 cut(s) 348, 429
Hin1II CATG 3 cut(s) 310, 1220, 1511
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 6 cut(s) 356, 669, 729, 860, 1015, 1243
HphI GGTGA 6 cut(s) 187, 464, 856, 988, 1024, 1327
Hpy166II GTNNAC 6 cut(s) 370, 427, 875, 900, 1032, 1188
Hpy188I TCNGA 9 cut(s) 325, 346, 361, 436, 915, 927, 1020, 1104, 1248
Hpy188III TCNNGA 5 cut(s) 384, 568, 643, 1082, 1439
Hpy8I GTNNAC 6 cut(s) 370, 427, 875, 900, 1032, 1188
Hpy99I CGWCG 7 cut(s) 129, 143, 146, 149, 295, 353, 605
HpyCH4III ACNGT 3 cut(s) 367, 793, 1000
HpyCH4IV ACGT 2 cut(s) 348, 584
HpyCH4V TGCA 7 cut(s) 331, 650, 1003, 1300, 1363, 1504, 1517
HpyF3I CTNAG 4 cut(s) 778, 1191, 1379, 1406
HpySE526I ACGT 2 cut(s) 348, 584
Hsp92I GRCGYC 2 cut(s) 348, 429
Hsp92II CATG 3 cut(s) 310, 1220, 1511
HspAI GCGC 1 cut(s) 444
Kpn2I TCCGGA 3 cut(s) 567, 642, 1438
KpnI GGTACC 1 cut(s) 1159
Ksp22I TGATCA 1 cut(s) 1072
Kzo9I GATC 5 cut(s) 189, 594, 915, 1072, 1454
LmnI GCTCC 4 cut(s) 214, 714, 928, 1326
Lsp1109I GCAGC 6 cut(s) 195, 229, 928, 1207, 1225, 1347
LweI GCATC 5 cut(s) 789, 1207, 1252, 1471, 1513
MaeI CTAG 4 cut(s) 38, 761, 984, 1534
MaeII ACGT 2 cut(s) 348, 584
MaeIII GTNAC 2 cut(s) 470, 524
MalI GATC 5 cut(s) 191, 596, 917, 1074, 1456
MbiI CCGCTC 1 cut(s) 709
MboI GATC 5 cut(s) 189, 594, 915, 1072, 1454
MboII GAAGA 9 cut(s) 284, 307, 557, 863, 940, 991, 1243, 1315, 1480
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 5 cut(s) 5, 551, 610, 651, 814
MlyI GAGTC 3 cut(s) 365, 678, 869
MroI TCCGGA 3 cut(s) 567, 642, 1438
MseI TTAA 2 cut(s) 951, 1125
MslI CAYNNNNRTG 2 cut(s) 998, 1037
MspA1I CMGCKG 1 cut(s) 478
MspR9I CCNGG 9 cut(s) 26, 156, 242, 453, 511, 542, 1056, 1111, 1325
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 3 cut(s) 511, 1056, 1325
MvnI CGCG 2 cut(s) 392, 446
NciI CCSGG 6 cut(s) 26, 156, 242, 453, 542, 1111
NcoI CCATGG 1 cut(s) 306
NdeII GATC 5 cut(s) 189, 594, 915, 1072, 1454
NlaIII CATG 3 cut(s) 310, 1220, 1511
NlaIV GGNNCC 5 cut(s) 30, 974, 1157, 1182, 1437
NmuCI GTSAC 1 cut(s) 470
NspI RCATGY 1 cut(s) 1220
OliI CACNNNNGTG 1 cut(s) 1037
PaeI GCATGC 1 cut(s) 1220
PceI AGGCCT 1 cut(s) 509
PcsI WCGNNNNNNNCGW 4 cut(s) 357, 620, 1217, 1472
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 3 cut(s) 729, 1015, 1243
PflMI CCANNNNNTGG 1 cut(s) 510
PfoI TCCNGGA 2 cut(s) 540, 1323
PinAI ACCGGT 1 cut(s) 498
PleI GAGTC 3 cut(s) 364, 677, 868
PpsI GAGTC 3 cut(s) 364, 677, 868
PpuMI RGGWCCY 2 cut(s) 178, 232
Psp124BI GAGCTC 1 cut(s) 609
Psp5II RGGWCCY 2 cut(s) 178, 232
Psp6I CCWGG 3 cut(s) 509, 1054, 1323
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 3 cut(s) 509, 1054, 1323
PspN4I GGNNCC 5 cut(s) 30, 974, 1157, 1182, 1437
PspPI GGNCC 9 cut(s) 28, 149, 178, 232, 243, 496, 501, 658, 1107
PspPPI RGGWCCY 2 cut(s) 178, 232
PstNI CAGNNNCTG 1 cut(s) 1081
RsaI GTAC 4 cut(s) 765, 809, 899, 1157
RsaNI GTAC 4 cut(s) 764, 808, 898, 1156
RseI CAYNNNNRTG 2 cut(s) 998, 1037
Rsr2I CGGWCCG 1 cut(s) 658
RsrII CGGWCCG 1 cut(s) 658
SacI GAGCTC 1 cut(s) 609
SaqAI TTAA 2 cut(s) 951, 1125
Sau3AI GATC 5 cut(s) 189, 594, 915, 1072, 1454
Sau96I GGNCC 9 cut(s) 28, 149, 178, 232, 243, 496, 501, 658, 1107
ScaI AGTACT 2 cut(s) 765, 809
SchI GAGTC 3 cut(s) 365, 678, 869
ScrFI CCNGG 9 cut(s) 26, 156, 242, 453, 511, 542, 1056, 1111, 1325
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 5 cut(s) 789, 1207, 1252, 1471, 1513
SfcI CTRYAG 1 cut(s) 618
SinI GGWCC 6 cut(s) 28, 178, 232, 496, 501, 658
SmiMI CAYNNNNRTG 2 cut(s) 998, 1037
SpeI ACTAGT 1 cut(s) 760
SphI GCATGC 1 cut(s) 1220
Sse9I AATT 5 cut(s) 5, 551, 610, 651, 814
SseBI AGGCCT 1 cut(s) 509
SspMI CTAG 4 cut(s) 38, 761, 984, 1534
SstI GAGCTC 1 cut(s) 609
StuI AGGCCT 1 cut(s) 509
StyD4I CCNGG 9 cut(s) 24, 154, 240, 451, 509, 540, 1054, 1109, 1323
StyI CCWWGG 3 cut(s) 300, 306, 504
TaaI ACNGT 3 cut(s) 367, 793, 1000
TaiI ACGT 2 cut(s) 351, 587
TasI AATT 5 cut(s) 5, 551, 610, 651, 814
TatI WGTACW 3 cut(s) 763, 807, 897
TauI GCSGC 6 cut(s) 395, 404, 413, 416, 419, 422
TfiI GAWTC 3 cut(s) 729, 1015, 1243
Tru1I TTAA 2 cut(s) 951, 1125
Tru9I TTAA 2 cut(s) 951, 1125
TscAI CASTG 1 cut(s) 1039
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 6 cut(s) 208, 217, 941, 1195, 1213, 1360
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 17, 113, 188
TspGWI ACGGA 1 cut(s) 1325
TspRI CASTG 1 cut(s) 1039
Van91I CCANNNNNTGG 1 cut(s) 510
VpaK11BI GGWCC 6 cut(s) 28, 178, 232, 496, 501, 658
XapI RAATTY 1 cut(s) 551
XceI RCATGY 1 cut(s) 1220
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmiI GTMKAC 2 cut(s) 874, 1187
XspI CTAG 4 cut(s) 38, 761, 984, 1534
ZraI GACGTC 1 cut(s) 349
ZrmI AGTACT 2 cut(s) 765, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.