pycom08g05470
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015028
Physical Location & Seq
Forward (+)
4194581 .. 4196287
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05470.1

Sequence Viewer

Length: 1707 bp
ATGTCACAAAACTTTCCTTCCGCCCCACACTTTCTCAGTTTGTTAATCTCCGCGTTCACACTTGCATTTTTCATATTTTTCAAAAAGCCAAACACTTTTTCTCCTTCTTTTCCTATTTTGCTTCTTCTTCTTCTTCTTCCTCAATTTCCTTCTTCTTGTTGTTGTTGTTTCTGCAAATCTGTACCTTATATGAAACTCGAGCTCCAGAGAGACCCAGCACCACACCCAACACACCCTGCAGCTGAAGGCCTATCCACCATCTACACCTTCCTCCAGAACTCTCTCCACGCGCAAATGAATGAGAACGGAAACGGAAACGCCAGGAAACGGCGTCGTAAAGACTCCAGTGGCGGCGAGAAGAAGAAGAAGGAGCTGAAGGGCGTAGTCGCCTCCTTGTCGCTGCTAGAAGAGGAGGAGAAGCAGGACGAGCGGAAGCACGACAGGGCATCGAATGACGACGCGTCCTTTATCCCAGAGAAGCACACGGAGAGAACCAAGGCCATGCTCGATTGCTACTCCGATTTCCAGGAGTGCTCCTCGGAGGTGGAAGAATCCGAAACGATGAAGCGGAGAAAATCGAGAAGCGCCGCCGTTTCGGCGGAAGCCGATGGTGATAAAGGTAAGCCGGGAGTCGGGTCGGGTCAACATCGGCGGCTGTGGGTCAAGGACAGGTCGAAAGCGTGGTGGGATGAGTGCAACCAGCCGGATTTTCCGGAATCGGAATTCAAAAAGGTGTTTCGGATGGGGAAATCCACGTTCGATTTGATATGCGAGGAGCTTAACTCCGCCATCGCGAAGGAGGACACGACGCTGCGGTCGGCGATTCCGGTGCGGCAGAGAGTGGCGGTGTGCCTGTGGCGGCTGGCCACCGGCGACCCGCTCCGCCTCGTGTCTAAACGATTCGGCCTCGGCATCTCGACCTGCCACAAGCTCGTTCTCGAGGTCTGCTCCGCGATTCGGACGGTTCTGATGCCAAAGTACCTGCAGTGGCCGGATGAGGCCGCGGCGAGGAAAATTAAAGACGAGTTCGAGGGTATTTCGGGGATTCCAAACGTTACTGGGTCTATGTACACCACGCACATACCCATCATCGCTCCCAAAATCAGCGTCGCTGCGTACTTCAATAAACGGCACACCGATAGGAACCAGAAGACTTCGTACTCGATCACCGTGCAGGGGGTGGTGGACGCCAAAGGCGTTTTTACCGACGTGTGCATCGGATGGCCAGGATCAATGCCGGATGATCAGGTGCTGGAGAAATCGGCGTTGAATCAGAGGGCTAACAATGGTCTGCTGAAGAACGTGTGGATTGTTGGGGGTGCTGGGTACCCTCTGATGGACTGGGTGCTGGTGCCGTACACCCAGCAGAACCTAACGTGGACCCAGCACGCTTTCAACGAGAAGATTGGGGAGATTCAGAATGTCGCCAGAGATGCGTTTGCTAGGTTGAAAGGGAGGTGGTTTTGCTTGCAGAAGAGGACGGAAGTGAAGCTGCAGGACTTGCCGGTGGTTCTCGGGGCGTGCTGTGTTTTGCACAACATTTGTGAGTTGAGGAAGGAGGAGATGGATCCCGAGCTCCGGTTCGAGCTCGTCGACGACGAGATGGTCCCGGAGGTCGCTCTGAGGTCGACGAGCTCGATGAAGGCCAGGGATGCCATTGCTCATAATTTATTGCACCACGGCCTCGCGGGTACTAGCTTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

569

Amino Acids

63.93

Weight (kDa)

8.54

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 357 - 513 3.3e-33 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 814, 1604
Acc36I ACCTGC 2 cut(s) 929, 990
Acc65I GGTACC 1 cut(s) 1326
AccB1I GGYRCC 2 cut(s) 1326, 1351
AccBSI CCGCTC 2 cut(s) 430, 880
AccI GTMKAC 2 cut(s) 1593, 1628
AccII CGCG 7 cut(s) 53, 290, 461, 794, 953, 1004, 1688
AccIII TCCGGA 1 cut(s) 712
AclI AACGTT 1 cut(s) 1053
AclWI GGATC 3 cut(s) 1237, 1562, 1575
AcoI YGGCCR 3 cut(s) 864, 989, 1223
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 3 cut(s) 264, 395, 1316
AcyI GRCGYC 2 cut(s) 331, 1188
AfaI GTAC 8 cut(s) 183, 980, 1070, 1118, 1160, 1328, 1358, 1693
AfiI CCNNNNNNNGG 7 cut(s) 327, 632, 957, 1008, 1176, 1336, 1578
AflIII ACRYGT 3 cut(s) 459, 1209, 1302
AgsI TTSAA 6 cut(s) 82, 727, 1123, 1270, 1396, 1450
AhdI GACNNNNNGTC 1 cut(s) 460
AjiI CACGTC 1 cut(s) 1210
AjnI CCWGG 4 cut(s) 320, 525, 1225, 1646
AjuI GAANNNNNNNTTGG 2 cut(s) 82, 114
Alw21I GWGCWC 5 cut(s) 204, 536, 1578, 1590, 1637
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 3 cut(s) 1237, 1562, 1575
AlwNI CAGNNNCTG 1 cut(s) 1252
Ama87I CYCGRG 4 cut(s) 197, 938, 1514, 1571
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 9 cut(s) 247, 498, 864, 904, 989, 999, 1223, 1644, 1681
ApeKI GCWGC 5 cut(s) 239, 400, 811, 1112, 1492
ApoI RAATTY 1 cut(s) 722
ArsI GACNNNNNNTTYG 2 cut(s) 1092, 1124
Asp718I GGTACC 1 cut(s) 1326
AspLEI GCGC 2 cut(s) 292, 587
AspS9I GGNCC 2 cut(s) 1380, 1606
AsuC2I CCSGG 2 cut(s) 627, 1610
AsuHPI GGTGA 2 cut(s) 623, 1159
AvaI CYCGRG 4 cut(s) 197, 938, 1514, 1571
AvaII GGWCC 2 cut(s) 1380, 1606
BalI TGGCCA 2 cut(s) 866, 1225
BamHI GGATCC 1 cut(s) 1567
BanI GGYRCC 2 cut(s) 1326, 1351
BanII GRGCYC 4 cut(s) 204, 1578, 1590, 1637
BarI GAAGNNNNNNTAC 2 cut(s) 1142, 1174
BauI CACGAG 1 cut(s) 887
BbsI GAAGAC 1 cut(s) 1157
Bbv12I GWGCWC 5 cut(s) 204, 536, 1578, 1590, 1637
BbvI GCAGC 5 cut(s) 251, 387, 798, 1099, 1479
BccI CCATC 9 cut(s) 266, 602, 736, 797, 1094, 1215, 1330, 1558, 1597
BceAI ACGGC 5 cut(s) 344, 575, 1145, 1339, 1696
BcgI CGANNNNNNTGC 4 cut(s) 811, 845, 1153, 1187
BciT130I CCWGG 4 cut(s) 322, 527, 1227, 1648
BclI TGATCA 1 cut(s) 1243
BcnI CCSGG 2 cut(s) 627, 1610
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 3 cut(s) 404, 1443, 1695
BfmI CTRYAG 3 cut(s) 237, 983, 1493
BfoI RGCGCY 1 cut(s) 588
BfuAI ACCTGC 2 cut(s) 929, 990
BglI GCCNNNNNGGC 1 cut(s) 596
Bme1390I CCNGG 6 cut(s) 322, 527, 627, 1227, 1610, 1648
Bme18I GGWCC 2 cut(s) 1380, 1606
BmeRI GACNNNNNGTC 1 cut(s) 460
BmeT110I CYCGRG 4 cut(s) 197, 938, 1514, 1571
BmgBI CACGTC 1 cut(s) 1210
BmgT120I GGNCC 2 cut(s) 1380, 1606
BmiI GGNNCC 6 cut(s) 1145, 1328, 1353, 1382, 1569, 1608
BmrFI CCNGG 6 cut(s) 322, 527, 627, 1227, 1610, 1648
BmrI ACTGGG 2 cut(s) 1068, 1351
BmsI GCATC 6 cut(s) 455, 921, 960, 1224, 1423, 1642
BmuI ACTGGG 2 cut(s) 1068, 1351
BpiI GAAGAC 1 cut(s) 1157
BplI GAGNNNNNCTC 6 cut(s) 521, 553, 767, 799, 932, 964
BpmI CTGGAG 4 cut(s) 188, 257, 328, 1274
BpuMI CCSGG 2 cut(s) 627, 1610
BsaHI GRCGYC 2 cut(s) 331, 1188
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 6 cut(s) 495, 537, 907, 1002, 1647, 1678
BsaWI WCCGGW 3 cut(s) 712, 826, 1578
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 186, 216
Bsc4I CCNNNNNNNGG 7 cut(s) 327, 632, 957, 1008, 1176, 1336, 1578
Bse118I RCCGGY 2 cut(s) 869, 1504
Bse1I ACTGG 3 cut(s) 345, 1063, 1346
Bse3DI GCAATG 1 cut(s) 1656
BseAI TCCGGA 1 cut(s) 712
BseBI CCWGG 4 cut(s) 322, 527, 1227, 1648
BseDI CCNNGG 6 cut(s) 495, 537, 907, 1002, 1647, 1678
BseGI GGATG 6 cut(s) 694, 747, 1000, 1226, 1246, 1657
BseLI CCNNNNNNNGG 7 cut(s) 327, 632, 957, 1008, 1176, 1336, 1578
BseMI GCAATG 1 cut(s) 1656
BseMII CTCAG 2 cut(s) 49, 1613
BseNI ACTGG 3 cut(s) 345, 1063, 1346
BseRI GAGGAG 5 cut(s) 425, 428, 526, 788, 1574
BseXI GCAGC 5 cut(s) 251, 387, 798, 1099, 1479
BseYI CCCAGC 4 cut(s) 214, 1322, 1362, 1383
BsgI GTGCAG 1 cut(s) 1193
Bsh1236I CGCG 7 cut(s) 53, 290, 461, 794, 953, 1004, 1688
Bsh1285I CGRYCG 1 cut(s) 818
BshFI GGCC 9 cut(s) 249, 500, 866, 906, 991, 1001, 1225, 1646, 1683
BshNI GGYRCC 2 cut(s) 1326, 1351
BsiEI CGRYCG 1 cut(s) 818
BsiHKAI GWGCWC 5 cut(s) 204, 536, 1578, 1590, 1637
BsiHKCI CYCGRG 4 cut(s) 197, 938, 1514, 1571
BslFI GGGAC 1 cut(s) 1592
BslI CCNNNNNNNGG 7 cut(s) 327, 632, 957, 1008, 1176, 1336, 1578
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 1592
BsnI GGCC 9 cut(s) 249, 500, 866, 906, 991, 1001, 1225, 1646, 1683
Bso31I GGTCTC 1 cut(s) 204
BsoBI CYCGRG 4 cut(s) 197, 938, 1514, 1571
Bsp1286I GDGCHC 5 cut(s) 204, 536, 1578, 1590, 1637
Bsp13I TCCGGA 1 cut(s) 712
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 4 cut(s) 1164, 1229, 1243, 1567
Bsp68I TCGCGA 1 cut(s) 794
BspANI GGCC 9 cut(s) 249, 500, 866, 906, 991, 1001, 1225, 1646, 1683
BspCNI CTCAG 2 cut(s) 48, 1614
BspEI TCCGGA 1 cut(s) 712
BspFNI CGCG 7 cut(s) 53, 290, 461, 794, 953, 1004, 1688
BspLI GGNNCC 6 cut(s) 1145, 1328, 1353, 1382, 1569, 1608
BspMAI CTGCAG 3 cut(s) 241, 987, 1497
BspMI ACCTGC 2 cut(s) 929, 990
BspPI GGATC 3 cut(s) 1237, 1562, 1575
BspT107I GGYRCC 2 cut(s) 1326, 1351
BspTNI GGTCTC 1 cut(s) 204
BsrBI CCGCTC 2 cut(s) 430, 880
BsrDI GCAATG 1 cut(s) 1656
BsrFI RCCGGY 2 cut(s) 869, 1504
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 3 cut(s) 345, 1063, 1346
BssAI RCCGGY 2 cut(s) 869, 1504
BssECI CCNNGG 6 cut(s) 495, 537, 907, 1002, 1647, 1678
BssMI GATC 4 cut(s) 1164, 1229, 1243, 1567
BssNI GRCGYC 2 cut(s) 331, 1188
BssSI CACGAG 1 cut(s) 887
BssT1I CCWWGG 1 cut(s) 495
Bst2BI CACGAG 1 cut(s) 887
Bst2UI CCWGG 4 cut(s) 322, 527, 1227, 1648
Bst4CI ACNGT 2 cut(s) 964, 1171
Bst6I CTCTTC 2 cut(s) 402, 1469
BstACI GRCGYC 2 cut(s) 331, 1188
BstAPI GCANNNNNTGC 1 cut(s) 1501
BstAUI TGTACA 1 cut(s) 1068
BstC8I GCNNGC 4 cut(s) 864, 1389, 1469, 1522
BstDEI CTNAG 2 cut(s) 35, 1622
BstDSI CCRYGG 2 cut(s) 1002, 1678
BstF5I GGATG 6 cut(s) 694, 747, 1000, 1226, 1246, 1657
BstFNI CGCG 7 cut(s) 53, 290, 461, 794, 953, 1004, 1688
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 2 cut(s) 292, 587
BstKTI GATC 4 cut(s) 1167, 1232, 1246, 1570
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 4 cut(s) 1164, 1229, 1243, 1567
BstMCI CGRYCG 1 cut(s) 818
BstMWI GCNNNNNNNGC 5 cut(s) 427, 596, 1433, 1501, 1652
BstNI CCWGG 4 cut(s) 322, 527, 1227, 1648
BstSCI CCNGG 6 cut(s) 320, 525, 625, 1225, 1608, 1646
BstSFI CTRYAG 3 cut(s) 237, 983, 1493
BstUI CGCG 7 cut(s) 53, 290, 461, 794, 953, 1004, 1688
BstV1I GCAGC 5 cut(s) 251, 387, 798, 1099, 1479
BstV2I GAAGAC 1 cut(s) 1157
BstX2I RGATCY 1 cut(s) 1567
BstYI RGATCY 1 cut(s) 1567
BsuRI GGCC 9 cut(s) 249, 500, 866, 906, 991, 1001, 1225, 1646, 1683
BtgI CCRYGG 2 cut(s) 1002, 1678
BtgZI GCGATG 2 cut(s) 775, 1075
BtrI CACGTC 1 cut(s) 1210
BtsCI GGATG 6 cut(s) 694, 747, 1000, 1226, 1246, 1657
BtsI GCAGTG 1 cut(s) 992
BtsIMutI CAGTG 2 cut(s) 352, 992
BtuMI TCGCGA 1 cut(s) 794
BveI ACCTGC 2 cut(s) 929, 990
Cac8I GCNNGC 4 cut(s) 864, 1389, 1469, 1522
CaiI CAGNNNCTG 1 cut(s) 1252
CfoI GCGC 2 cut(s) 292, 587
Cfr10I RCCGGY 2 cut(s) 869, 1504
Cfr13I GGNCC 2 cut(s) 1380, 1606
Cfr42I CCGCGG 1 cut(s) 1005
CseI GACGC 6 cut(s) 320, 450, 467, 817, 1096, 1196
Csp6I GTAC 8 cut(s) 182, 979, 1069, 1117, 1159, 1327, 1357, 1692
CviAII CATG 1 cut(s) 502
CviQI GTAC 8 cut(s) 182, 979, 1069, 1117, 1159, 1327, 1357, 1692
DdeI CTNAG 2 cut(s) 35, 1622
DpnI GATC 4 cut(s) 1166, 1231, 1245, 1569
DpnII GATC 4 cut(s) 1164, 1229, 1243, 1567
DrdI GACNNNNNNGTC 2 cut(s) 814, 1604
DriI GACNNNNNGTC 1 cut(s) 460
DseDI GACNNNNNNGTC 2 cut(s) 814, 1604
EaeI YGGCCR 3 cut(s) 864, 989, 1223
Eam1104I CTCTTC 2 cut(s) 402, 1469
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 2 cut(s) 402, 1469
EciI GGCGGA 4 cut(s) 10, 614, 775, 872
Ecl136II GAGCTC 4 cut(s) 202, 1576, 1588, 1635
Eco130I CCWWGG 1 cut(s) 495
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 4 cut(s) 204, 1578, 1590, 1637
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 1380, 1606
Eco53kI GAGCTC 4 cut(s) 202, 1576, 1588, 1635
Eco57I CTGAAG 3 cut(s) 264, 395, 1316
Eco88I CYCGRG 4 cut(s) 197, 938, 1514, 1571
EcoICRI GAGCTC 4 cut(s) 202, 1576, 1588, 1635
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 4 cut(s) 320, 525, 1225, 1646
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 4 cut(s) 204, 1578, 1590, 1637
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 1 cut(s) 505
FaiI YATR 8 cut(s) 74, 189, 191, 503, 769, 1067, 1082, 1665
FaqI GGGAC 1 cut(s) 1592
FatI CATG 1 cut(s) 501
FauI CCCGC 2 cut(s) 885, 1681
FbaI TGATCA 1 cut(s) 1243
FblI GTMKAC 2 cut(s) 1593, 1628
FokI GGATG 6 cut(s) 701, 754, 1007, 1233, 1253, 1664
FriOI GRGCYC 4 cut(s) 204, 1578, 1590, 1637
FspBI CTAG 3 cut(s) 404, 1443, 1695
GlaI GCGC 2 cut(s) 291, 586
GsaI CCCAGC 4 cut(s) 218, 1326, 1366, 1387
GsuI CTGGAG 4 cut(s) 188, 257, 328, 1274
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 9 cut(s) 249, 500, 866, 906, 991, 1001, 1225, 1646, 1683
HgaI GACGC 6 cut(s) 320, 450, 467, 817, 1096, 1196
HhaI GCGC 2 cut(s) 292, 587
Hin1I GRCGYC 2 cut(s) 331, 1188
Hin1II CATG 1 cut(s) 505
Hin6I GCGC 2 cut(s) 290, 585
HinP1I GCGC 2 cut(s) 290, 585
HincII GTYRAC 3 cut(s) 644, 1594, 1629
HindII GTYRAC 3 cut(s) 644, 1594, 1629
HphI GGTGA 2 cut(s) 623, 1159
Hpy166II GTNNAC 8 cut(s) 57, 644, 1071, 1186, 1359, 1380, 1594, 1629
Hpy188III TCNNGA 8 cut(s) 205, 274, 579, 713, 793, 916, 938, 1571
Hpy8I GTNNAC 8 cut(s) 57, 644, 1071, 1186, 1359, 1380, 1594, 1629
Hpy99I CGWCG 9 cut(s) 336, 461, 811, 1112, 1211, 1595, 1598, 1601, 1633
HpyCH4III ACNGT 2 cut(s) 964, 1171
HpyCH4IV ACGT 5 cut(s) 755, 1053, 1209, 1302, 1376
HpyF10VI GCNNNNNNNGC 5 cut(s) 427, 596, 1433, 1501, 1652
HpyF3I CTNAG 2 cut(s) 35, 1622
HpySE526I ACGT 5 cut(s) 755, 1053, 1209, 1302, 1376
Hsp92I GRCGYC 2 cut(s) 331, 1188
Hsp92II CATG 1 cut(s) 505
HspAI GCGC 2 cut(s) 290, 585
Kpn2I TCCGGA 1 cut(s) 712
KpnI GGTACC 1 cut(s) 1330
Ksp22I TGATCA 1 cut(s) 1243
KspI CCGCGG 1 cut(s) 1005
Kzo9I GATC 4 cut(s) 1164, 1229, 1243, 1567
LmnI GCTCC 8 cut(s) 207, 370, 539, 775, 885, 953, 1099, 1581
Lsp1109I GCAGC 5 cut(s) 251, 387, 798, 1099, 1479
LweI GCATC 6 cut(s) 455, 921, 960, 1224, 1423, 1642
MaeI CTAG 3 cut(s) 404, 1443, 1695
MaeII ACGT 5 cut(s) 755, 1053, 1209, 1302, 1376
MaeIII GTNAC 2 cut(s) 3, 1054
MalI GATC 4 cut(s) 1166, 1231, 1245, 1569
MbiI CCGCTC 2 cut(s) 430, 880
MboI GATC 4 cut(s) 1164, 1229, 1243, 1567
MflI RGATCY 1 cut(s) 1567
MhlI GDGCHC 5 cut(s) 204, 536, 1578, 1590, 1637
MlsI TGGCCA 2 cut(s) 866, 1225
MluCI AATT 4 cut(s) 143, 722, 1014, 1666
MluI ACGCGT 1 cut(s) 459
MluNI TGGCCA 2 cut(s) 866, 1225
MlyI GAGTC 2 cut(s) 335, 639
Mox20I TGGCCA 2 cut(s) 866, 1225
MroI TCCGGA 1 cut(s) 712
MscI TGGCCA 2 cut(s) 866, 1225
MseI TTAA 3 cut(s) 44, 780, 1017
Msp20I TGGCCA 2 cut(s) 866, 1225
MspA1I CMGCKG 2 cut(s) 242, 1004
MspR9I CCNGG 6 cut(s) 322, 527, 627, 1227, 1610, 1648
MvaI CCWGG 4 cut(s) 322, 527, 1227, 1648
MvnI CGCG 7 cut(s) 53, 290, 461, 794, 953, 1004, 1688
MwoI GCNNNNNNNGC 5 cut(s) 427, 596, 1433, 1501, 1652
NciI CCSGG 2 cut(s) 627, 1610
NdeII GATC 4 cut(s) 1164, 1229, 1243, 1567
NlaIII CATG 1 cut(s) 505
NlaIV GGNNCC 6 cut(s) 1145, 1328, 1353, 1382, 1569, 1608
NmeAIII GCCGAG 1 cut(s) 888
NmuCI GTSAC 1 cut(s) 3
NruI TCGCGA 1 cut(s) 794
PaeR7I CTCGAG 2 cut(s) 197, 938
PceI AGGCCT 1 cut(s) 249
PcsI WCGNNNNNNNCGW 5 cut(s) 1194, 1395, 1590, 1596, 1634
PfeI GAWTC 8 cut(s) 551, 716, 823, 900, 955, 1045, 1270, 1414
PfoI TCCNGGA 2 cut(s) 525, 1608
PleI GAGTC 2 cut(s) 335, 638
PpsI GAGTC 2 cut(s) 335, 638
Psp124BI GAGCTC 4 cut(s) 204, 1578, 1590, 1637
Psp1406I AACGTT 1 cut(s) 1053
Psp6I CCWGG 4 cut(s) 320, 525, 1225, 1646
PspFI CCCAGC 4 cut(s) 214, 1322, 1362, 1383
PspGI CCWGG 4 cut(s) 320, 525, 1225, 1646
PspN4I GGNNCC 6 cut(s) 1145, 1328, 1353, 1382, 1569, 1608
PspPI GGNCC 2 cut(s) 1380, 1606
PspXI VCTCGAGB 1 cut(s) 197
PstI CTGCAG 3 cut(s) 241, 987, 1497
PstNI CAGNNNCTG 1 cut(s) 1252
PsuI RGATCY 1 cut(s) 1567
PvuII CAGCTG 1 cut(s) 242
RruI TCGCGA 1 cut(s) 794
RsaI GTAC 8 cut(s) 183, 980, 1070, 1118, 1160, 1328, 1358, 1693
RsaNI GTAC 8 cut(s) 182, 979, 1069, 1117, 1159, 1327, 1357, 1692
SacI GAGCTC 4 cut(s) 204, 1578, 1590, 1637
SacII CCGCGG 1 cut(s) 1005
SalI GTCGAC 2 cut(s) 1592, 1627
SaqAI TTAA 3 cut(s) 44, 780, 1017
Sau3AI GATC 4 cut(s) 1164, 1229, 1243, 1567
Sau96I GGNCC 2 cut(s) 1380, 1606
SchI GAGTC 2 cut(s) 335, 639
ScrFI CCNGG 6 cut(s) 322, 527, 627, 1227, 1610, 1648
SduI GDGCHC 5 cut(s) 204, 536, 1578, 1590, 1637
SfaNI GCATC 6 cut(s) 455, 921, 960, 1224, 1423, 1642
SfcI CTRYAG 3 cut(s) 237, 983, 1493
Sfr274I CTCGAG 2 cut(s) 197, 938
Sfr303I CCGCGG 1 cut(s) 1005
SgrAI CRCCGGYG 1 cut(s) 869
SgrBI CCGCGG 1 cut(s) 1005
SgrDI CGTCGACG 1 cut(s) 1592
SinI GGWCC 2 cut(s) 1380, 1606
SlaI CTCGAG 2 cut(s) 197, 938
SmlI CTYRAG 2 cut(s) 197, 938
SmoI CTYRAG 2 cut(s) 197, 938
Sse9I AATT 4 cut(s) 143, 722, 1014, 1666
SseBI AGGCCT 1 cut(s) 249
SspMI CTAG 3 cut(s) 404, 1443, 1695
SstI GAGCTC 4 cut(s) 204, 1578, 1590, 1637
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 6 cut(s) 320, 525, 625, 1225, 1608, 1646
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 964, 1171
TaiI ACGT 5 cut(s) 758, 1056, 1212, 1305, 1379
TasI AATT 4 cut(s) 143, 722, 1014, 1666
TatI WGTACW 1 cut(s) 1068
TauI GCSGC 7 cut(s) 354, 590, 655, 835, 862, 1004, 1007
TfiI GAWTC 8 cut(s) 551, 716, 823, 900, 955, 1045, 1270, 1414
Tru1I TTAA 3 cut(s) 44, 780, 1017
Tru9I TTAA 3 cut(s) 44, 780, 1017
TscAI CASTG 2 cut(s) 352, 992
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 5 cut(s) 239, 400, 811, 1112, 1492
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 5 cut(s) 61, 206, 311, 578, 1655
TspGWI ACGGA 4 cut(s) 321, 327, 500, 1496
TspRI CASTG 2 cut(s) 352, 992
VpaK11BI GGWCC 2 cut(s) 1380, 1606
XapI RAATTY 1 cut(s) 722
XhoI CTCGAG 2 cut(s) 197, 938
XmiI GTMKAC 2 cut(s) 1593, 1628
XspI CTAG 3 cut(s) 404, 1443, 1695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.