Rw6G043730
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015028
Physical Location & Seq
Forward (+)
66953977 .. 66955511
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043730.1

Sequence Viewer

Length: 1494 bp
ATGAAATTGAAACCCATACCCATACCCAACCCAGACCAACCCGACCTCTCCTACATCTACTCCTTCCTCCACTCCTCTCTCCACCACCAAATGAACAATGATACCGCCAAGAAACGCCGTCGTAAGACCGCCGACGACGACGGCCCGCCGGGAAAGCAAGGCAAGATGAAGGACCTGAAGGGGATCATCACCACCCTGTCGCTGCTGGAGCAGCAGGAGAAGGAGGACCTCGACCGGGCCTCAAATGACGACAAGTCGTTTGCGGAAGAGAAGCAGAAGCAGACGACGAAGACCAAGGCCATGGTGGAACACTACTCCGATTTGCAGGGCTACTACTCGGACGTCGAGGAGTCCGAGACCGTGAACCGCAAGAAGTCGAGAAACGCGGCGACCGCGGCCGGCGCGAAACCCGGAGGAAGCGGGTCGGGTCACCAGCGGAGGCTTTGGGTCAAGGACCGGTCCAAGGCCTGGTGGGACGAGTGTAACCAACCCGATTTCCCGGAAGAGGAATTCAGAAAAGCCTTCCGGATGGGGAAGGCCACGTTCGATTTGATCTGCGACGAGCTCAATTCGGCTATAGCGAAAGAGGACACCACTCTCCGGAATGCAATTCCGGTCCGGCAGAGAGTCGCCGTCTGTTTGTGGAGGTTAGCTACCGGCGACCCGCTCCGGCTCGTTTCGAAGCGATTCGGGTTAGGGATATCGACTTGTCATAAGCTAGTACTCGAAGTCTGCTCAGCTATTAGGACTGTGTTGATGCCCAAGTACTTGAATTGGCCGGAGGATGGTGTTTTGAGGAAGGTGAAGGACGAGTTCGAGTCGTTTTCGGGTATACCTAATGTTGTAGGGTCTATGTACACTACTCATATTCCGATCATAGCTCCGAAGATTAGTGTGGCAGCTTATTTTAACAAGAGGCATACAGAGAGGAACCAGAAGACTAGCTACTCCATCACCGTGCAAGGTGTGGTGAATCCGAAAGGGGTGTTCACTGATGTGTGTATAGGGTGGCCTGGTTCAATGCCGGATGATCAGGTTCTGGAGAAGTCGGCTTTGCATCAGAGGGCCGGGAATGGTTTGCTTAAAGGTGTGTGGATTGTGGGGGGTTCGGGGTACCCTTTGCTGGATTGGGTGCTGGTGCCCTATACTCAGCAGCATCTTACTTGGACGCAGCATGCGTTCAATGAGAAGATTGGGGAGATTCAGAATGTGTCGAAAGATGCGTTTGCGAGGTTGAAGGGGAGGTGGTTGTGCTTGCAGAAGAGGACCGAGGTGAAGCTCCAGGACTTGCCTGTGGTTCTTGGGGCTTGCTGTGTGCTGCACAATATATGCGAGCTGAGGAATGAGGAAATCGACCCCGAGCTGAGGTTTGAGCTTGTGGATGATGAGATGGTTCCGGACATGGCTGTGAGATCACAAAGCTCGAAGAAGGCGAGGGATGCCATTGCTCATAATCTTTTGCATCATGGCCTTGCAGGCACTTCTTTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

497

Amino Acids

56.03

Weight (kDa)

8.72

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 168 - 248 9.1e-06 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 286 - 442 1e-34 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
Acc65I GGTACC 1 cut(s) 1113
AccB1I GGYRCC 2 cut(s) 1113, 1138
AccB7I CCANNNNNTGG 1 cut(s) 468
AccBSI CCGCTC 1 cut(s) 667
AccI GTMKAC 1 cut(s) 832
AccII CGCG 3 cut(s) 386, 395, 404
AccIII TCCGGA 3 cut(s) 525, 600, 1396
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 2 cut(s) 396, 776
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 1 cut(s) 197
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 4 cut(s) 723, 767, 857, 1115
AfiI CCNNNNNNNGG 8 cut(s) 235, 463, 468, 505, 600, 785, 1123, 1365
AgeI ACCGGT 1 cut(s) 456
AgsI TTSAA 5 cut(s) 10, 772, 1020, 1183, 1237
AhdI GACNNNNNGTC 1 cut(s) 253
AjnI CCWGG 3 cut(s) 467, 1012, 1281
AleI CACNNNNGTG 1 cut(s) 995
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 1039
Ama87I CYCGRG 1 cut(s) 1358
Aor13HI TCCGGA 3 cut(s) 525, 600, 1396
ApeKI GCWGC 6 cut(s) 202, 211, 899, 1153, 1171, 1318
ApoI RAATTY 1 cut(s) 509
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AsiGI ACCGGT 1 cut(s) 456
Asp700I GAANNNNTTC 1 cut(s) 686
Asp718I GGTACC 1 cut(s) 1113
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 9 cut(s) 143, 172, 226, 237, 454, 459, 616, 1065, 1266
AsuC2I CCSGG 5 cut(s) 150, 236, 411, 500, 1069
AsuHPI GGTGA 6 cut(s) 181, 422, 814, 946, 982, 1285
AsuII TTCGAA 1 cut(s) 680
AvaI CYCGRG 1 cut(s) 1358
AvaII GGWCC 6 cut(s) 172, 226, 454, 459, 616, 1266
BaeGI GKGCMC 1 cut(s) 1143
BanI GGYRCC 2 cut(s) 1113, 1138
BanII GRGCYC 1 cut(s) 567
BarI GAAGNNNNNNTAC 2 cut(s) 929, 961
BbsI GAAGAC 2 cut(s) 296, 944
Bbv12I GWGCWC 1 cut(s) 567
BbvCI CCTCAGC 2 cut(s) 1337, 1364
BbvI GCAGC 6 cut(s) 189, 223, 911, 1165, 1183, 1305
BccI CCATC 4 cut(s) 523, 779, 959, 1384
BceAI ACGGC 3 cut(s) 102, 157, 617
BciT130I CCWGG 3 cut(s) 469, 1014, 1283
BclI TGATCA 1 cut(s) 1030
BcnI CCSGG 5 cut(s) 150, 236, 411, 500, 1069
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 3 cut(s) 719, 942, 1492
BfmI CTRYAG 1 cut(s) 576
BglI GCCNNNNNGGC 1 cut(s) 1476
BisI GCNGC 8 cut(s) 203, 212, 387, 396, 900, 1154, 1172, 1319
BlpI GCTNAGC 1 cut(s) 736
BlsI GCNGC 8 cut(s) 204, 213, 388, 397, 901, 1155, 1173, 1320
BmcAI AGTACT 2 cut(s) 723, 767
Bme1390I CCNGG 8 cut(s) 150, 236, 411, 469, 500, 1014, 1069, 1283
Bme18I GGWCC 6 cut(s) 172, 226, 454, 459, 616, 1266
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 1358
BmgT120I GGNCC 9 cut(s) 143, 172, 226, 237, 454, 459, 616, 1065, 1266
BmiI GGNNCC 4 cut(s) 932, 1115, 1140, 1395
BmrFI CCNGG 8 cut(s) 150, 236, 411, 469, 500, 1014, 1069, 1283
BmsI GCATC 6 cut(s) 747, 1066, 1165, 1210, 1429, 1471
BpiI GAAGAC 2 cut(s) 296, 944
BpmI CTGGAG 3 cut(s) 227, 1061, 1265
Bpu10I CCTNAGC 2 cut(s) 1337, 1364
Bpu1102I GCTNAGC 1 cut(s) 736
Bpu14I TTCGAA 1 cut(s) 680
BpuMI CCSGG 5 cut(s) 150, 236, 411, 500, 1069
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 5 cut(s) 294, 300, 393, 462, 1269
BsaWI WCCGGW 5 cut(s) 456, 525, 600, 613, 1396
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 8 cut(s) 235, 463, 468, 505, 600, 785, 1123, 1365
Bse118I RCCGGY 3 cut(s) 398, 456, 656
Bse3DI GCAATG 1 cut(s) 1443
BseAI TCCGGA 3 cut(s) 525, 600, 1396
BseBI CCWGG 3 cut(s) 469, 1014, 1283
BseDI CCNNGG 5 cut(s) 294, 300, 393, 462, 1269
BseGI GGATG 5 cut(s) 534, 790, 1033, 1387, 1444
BseLI CCNNNNNNNGG 8 cut(s) 235, 463, 468, 505, 600, 785, 1123, 1365
BseMI GCAATG 1 cut(s) 1443
BseMII CTCAG 4 cut(s) 750, 1163, 1328, 1355
BseRI GAGGAG 2 cut(s) 64, 362
BseSI GKGCMC 1 cut(s) 1143
BseX3I CGGCCG 1 cut(s) 396
BseXI GCAGC 6 cut(s) 189, 223, 911, 1165, 1183, 1305
BsgI GTGCAG 1 cut(s) 1304
Bsh1236I CGCG 3 cut(s) 386, 395, 404
Bsh1285I CGRYCG 3 cut(s) 235, 393, 399
BshNI GGYRCC 2 cut(s) 1113, 1138
BshTI ACCGGT 1 cut(s) 456
BsiEI CGRYCG 3 cut(s) 235, 393, 399
BsiHKAI GWGCWC 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 1358
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 8 cut(s) 235, 463, 468, 505, 600, 785, 1123, 1365
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 488
BsmI GAATGC 1 cut(s) 610
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 1358
Bsp119I TTCGAA 1 cut(s) 680
Bsp1286I GDGCHC 2 cut(s) 567, 1143
Bsp13I TCCGGA 3 cut(s) 525, 600, 1396
Bsp1407I TGTACA 1 cut(s) 855
Bsp143I GATC 5 cut(s) 183, 552, 873, 1030, 1412
Bsp1720I GCTNAGC 1 cut(s) 736
Bsp19I CCATGG 1 cut(s) 300
BspCNI CTCAG 4 cut(s) 749, 1162, 1329, 1356
BspEI TCCGGA 3 cut(s) 525, 600, 1396
BspFNI CGCG 3 cut(s) 386, 395, 404
BspLI GGNNCC 4 cut(s) 932, 1115, 1140, 1395
BspPI GGATC 1 cut(s) 191
BspT104I TTCGAA 1 cut(s) 680
BspT107I GGYRCC 2 cut(s) 1113, 1138
BspTNI GGTCTC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 667
BsrDI GCAATG 1 cut(s) 1443
BsrFI RCCGGY 3 cut(s) 398, 456, 656
BsrGI TGTACA 1 cut(s) 855
BssAI RCCGGY 3 cut(s) 398, 456, 656
BssECI CCNNGG 5 cut(s) 294, 300, 393, 462, 1269
BssMI GATC 5 cut(s) 183, 552, 873, 1030, 1412
BssNAI GTATAC 1 cut(s) 833
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 3 cut(s) 294, 300, 462
Bst1107I GTATAC 1 cut(s) 833
Bst2UI CCWGG 3 cut(s) 469, 1014, 1283
Bst4CI ACNGT 3 cut(s) 361, 751, 958
Bst6I CTCTTC 3 cut(s) 261, 498, 1256
BstACI GRCGYC 1 cut(s) 342
BstAUI TGTACA 1 cut(s) 855
BstBI TTCGAA 1 cut(s) 680
BstC8I GCNNGC 7 cut(s) 146, 400, 1176, 1256, 1309, 1334, 1477
BstDEI CTNAG 4 cut(s) 736, 1149, 1337, 1364
BstDSI CCRYGG 2 cut(s) 300, 393
BstEII GGTNACC 1 cut(s) 428
BstF5I GGATG 5 cut(s) 534, 790, 1033, 1387, 1444
BstFNI CGCG 3 cut(s) 386, 395, 404
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 5 cut(s) 186, 555, 876, 1033, 1415
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 5 cut(s) 183, 552, 873, 1030, 1412
BstMCI CGRYCG 3 cut(s) 235, 393, 399
BstMWI GCNNNNNNNGC 8 cut(s) 154, 208, 211, 392, 395, 401, 1439, 1476
BstNI CCWGG 3 cut(s) 469, 1014, 1283
BstNSI RCATGY 1 cut(s) 1178
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 8 cut(s) 148, 234, 409, 467, 498, 1012, 1067, 1281
BstSFI CTRYAG 1 cut(s) 576
BstSLI GKGCMC 1 cut(s) 1143
BstUI CGCG 3 cut(s) 386, 395, 404
BstV1I GCAGC 6 cut(s) 189, 223, 911, 1165, 1183, 1305
BstV2I GAAGAC 2 cut(s) 296, 944
BstXI CCANNNNNNTGG 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 833
BstZI CGGCCG 1 cut(s) 396
BtgI CCRYGG 2 cut(s) 300, 393
BtsCI GGATG 5 cut(s) 534, 790, 1033, 1387, 1444
BtsIMutI CAGTG 1 cut(s) 990
Cac8I GCNNGC 7 cut(s) 146, 400, 1176, 1256, 1309, 1334, 1477
CaiI CAGNNNCTG 1 cut(s) 1039
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 3 cut(s) 398, 456, 656
Cfr13I GGNCC 9 cut(s) 143, 172, 226, 237, 454, 459, 616, 1065, 1266
Cfr42I CCGCGG 1 cut(s) 396
CpoI CGGWCCG 1 cut(s) 616
CseI GACGC 1 cut(s) 1177
Csp6I GTAC 4 cut(s) 722, 766, 856, 1114
CspAI ACCGGT 1 cut(s) 456
CspI CGGWCCG 1 cut(s) 616
CviAII CATG 4 cut(s) 301, 1175, 1402, 1466
CviQI GTAC 4 cut(s) 722, 766, 856, 1114
DdeI CTNAG 4 cut(s) 736, 1149, 1337, 1364
DpnI GATC 5 cut(s) 185, 554, 875, 1032, 1414
DpnII GATC 5 cut(s) 183, 552, 873, 1030, 1412
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 2 cut(s) 396, 776
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 261, 498, 1256
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 3 cut(s) 261, 498, 1256
Ecl136II GAGCTC 1 cut(s) 565
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 3 cut(s) 294, 300, 462
Eco147I AGGCCT 1 cut(s) 467
Eco24I GRGCYC 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 350
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 6 cut(s) 172, 226, 454, 459, 616, 1266
Eco52I CGGCCG 1 cut(s) 396
Eco53kI GAGCTC 1 cut(s) 565
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 1358
Eco91I GGTNACC 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 565
EcoO109I RGGNCCY 2 cut(s) 172, 226
EcoO65I GGTNACC 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 467, 1012, 1281
EcoRV GATATC 1 cut(s) 702
EcoT14I CCWWGG 3 cut(s) 294, 300, 462
EcoT38I GRGCYC 1 cut(s) 567
ErhI CCWWGG 3 cut(s) 294, 300, 462
FaeI CATG 4 cut(s) 304, 1178, 1405, 1469
FaqI GGGAC 1 cut(s) 488
FatI CATG 4 cut(s) 300, 1174, 1401, 1465
FauI CCCGC 3 cut(s) 153, 413, 672
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 832
Fnu4HI GCNGC 8 cut(s) 203, 212, 387, 396, 900, 1154, 1172, 1319
FokI GGATG 5 cut(s) 541, 797, 1040, 1394, 1451
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 8 cut(s) 203, 212, 387, 396, 900, 1154, 1172, 1319
FspBI CTAG 3 cut(s) 719, 942, 1492
GlaI GCGC 1 cut(s) 403
GluI GCNGC 8 cut(s) 203, 212, 387, 396, 900, 1154, 1172, 1319
GsuI CTGGAG 3 cut(s) 227, 1061, 1265
HgaI GACGC 1 cut(s) 1177
HhaI GCGC 1 cut(s) 404
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 4 cut(s) 304, 1178, 1405, 1469
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 6 cut(s) 350, 627, 687, 818, 973, 1201
HphI GGTGA 6 cut(s) 181, 422, 814, 946, 982, 1285
Hpy166II GTNNAC 4 cut(s) 364, 833, 858, 990
Hpy188I TCNGA 9 cut(s) 319, 340, 355, 515, 873, 885, 978, 1062, 1206
Hpy188III TCNNGA 5 cut(s) 378, 526, 601, 1040, 1397
Hpy8I GTNNAC 4 cut(s) 364, 833, 858, 990
Hpy99I CGWCG 7 cut(s) 123, 137, 140, 143, 289, 347, 563
HpyCH4III ACNGT 3 cut(s) 361, 751, 958
HpyCH4IV ACGT 2 cut(s) 342, 542
HpyCH4V TGCA 8 cut(s) 325, 608, 961, 1057, 1258, 1321, 1462, 1475
HpyF10VI GCNNNNNNNGC 8 cut(s) 154, 208, 211, 392, 395, 401, 1439, 1476
HpyF3I CTNAG 4 cut(s) 736, 1149, 1337, 1364
HpySE526I ACGT 2 cut(s) 342, 542
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 4 cut(s) 304, 1178, 1405, 1469
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 3 cut(s) 525, 600, 1396
KpnI GGTACC 1 cut(s) 1117
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
Ksp22I TGATCA 1 cut(s) 1030
KspI CCGCGG 1 cut(s) 396
Kzo9I GATC 5 cut(s) 183, 552, 873, 1030, 1412
LmnI GCTCC 4 cut(s) 208, 672, 886, 1284
Lsp1109I GCAGC 6 cut(s) 189, 223, 911, 1165, 1183, 1305
LweI GCATC 6 cut(s) 747, 1066, 1165, 1210, 1429, 1471
MaeI CTAG 3 cut(s) 719, 942, 1492
MaeII ACGT 2 cut(s) 342, 542
MaeIII GTNAC 2 cut(s) 428, 482
MalI GATC 5 cut(s) 185, 554, 875, 1032, 1414
MbiI CCGCTC 1 cut(s) 667
MboI GATC 5 cut(s) 183, 552, 873, 1030, 1412
MboII GAAGA 8 cut(s) 278, 301, 515, 898, 949, 1201, 1273, 1438
MhlI GDGCHC 2 cut(s) 567, 1143
MluCI AATT 5 cut(s) 5, 509, 568, 609, 772
MlyI GAGTC 3 cut(s) 359, 636, 827
MroI TCCGGA 3 cut(s) 525, 600, 1396
MroNI GCCGGC 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 686
MseI TTAA 2 cut(s) 909, 1083
MslI CAYNNNNRTG 3 cut(s) 956, 995, 1406
MspA1I CMGCKG 2 cut(s) 395, 436
MspR9I CCNGG 8 cut(s) 150, 236, 411, 469, 500, 1014, 1069, 1283
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 3 cut(s) 469, 1014, 1283
MvnI CGCG 3 cut(s) 386, 395, 404
MwoI GCNNNNNNNGC 8 cut(s) 154, 208, 211, 392, 395, 401, 1439, 1476
NaeI GCCGGC 1 cut(s) 400
NciI CCSGG 5 cut(s) 150, 236, 411, 500, 1069
NcoI CCATGG 1 cut(s) 300
NdeII GATC 5 cut(s) 183, 552, 873, 1030, 1412
NgoMIV GCCGGC 1 cut(s) 398
NlaIII CATG 4 cut(s) 304, 1178, 1405, 1469
NlaIV GGNNCC 4 cut(s) 932, 1115, 1140, 1395
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 1 cut(s) 1178
NspV TTCGAA 1 cut(s) 680
OliI CACNNNNGTG 1 cut(s) 995
PaeI GCATGC 1 cut(s) 1178
PceI AGGCCT 1 cut(s) 467
PcsI WCGNNNNNNNCGW 5 cut(s) 351, 578, 1175, 1220, 1430
PctI GAATGC 1 cut(s) 610
PdiI GCCGGC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 3 cut(s) 687, 973, 1201
PflMI CCANNNNNTGG 1 cut(s) 468
PfoI TCCNGGA 2 cut(s) 498, 1281
PinAI ACCGGT 1 cut(s) 456
PkrI GCNGC 8 cut(s) 204, 213, 388, 397, 901, 1155, 1173, 1320
PleI GAGTC 3 cut(s) 358, 635, 826
PpsI GAGTC 3 cut(s) 358, 635, 826
PpuMI RGGWCCY 2 cut(s) 172, 226
Psp124BI GAGCTC 1 cut(s) 567
Psp5II RGGWCCY 2 cut(s) 172, 226
Psp6I CCWGG 3 cut(s) 467, 1012, 1281
PspEI GGTNACC 1 cut(s) 428
PspGI CCWGG 3 cut(s) 467, 1012, 1281
PspN4I GGNNCC 4 cut(s) 932, 1115, 1140, 1395
PspPI GGNCC 9 cut(s) 143, 172, 226, 237, 454, 459, 616, 1065, 1266
PspPPI RGGWCCY 2 cut(s) 172, 226
PstNI CAGNNNCTG 1 cut(s) 1039
RsaI GTAC 4 cut(s) 723, 767, 857, 1115
RsaNI GTAC 4 cut(s) 722, 766, 856, 1114
RseI CAYNNNNRTG 3 cut(s) 956, 995, 1406
Rsr2I CGGWCCG 1 cut(s) 616
RsrII CGGWCCG 1 cut(s) 616
SacI GAGCTC 1 cut(s) 567
SacII CCGCGG 1 cut(s) 396
SaqAI TTAA 2 cut(s) 909, 1083
SatI GCNGC 8 cut(s) 203, 212, 387, 396, 900, 1154, 1172, 1319
Sau3AI GATC 5 cut(s) 183, 552, 873, 1030, 1412
Sau96I GGNCC 9 cut(s) 143, 172, 226, 237, 454, 459, 616, 1065, 1266
ScaI AGTACT 2 cut(s) 723, 767
SchI GAGTC 3 cut(s) 359, 636, 827
ScrFI CCNGG 8 cut(s) 150, 236, 411, 469, 500, 1014, 1069, 1283
SduI GDGCHC 2 cut(s) 567, 1143
SfaNI GCATC 6 cut(s) 747, 1066, 1165, 1210, 1429, 1471
SfcI CTRYAG 1 cut(s) 576
Sfr303I CCGCGG 1 cut(s) 396
SfuI TTCGAA 1 cut(s) 680
SgrBI CCGCGG 1 cut(s) 396
SinI GGWCC 6 cut(s) 172, 226, 454, 459, 616, 1266
SmiMI CAYNNNNRTG 3 cut(s) 956, 995, 1406
SphI GCATGC 1 cut(s) 1178
Sse9I AATT 5 cut(s) 5, 509, 568, 609, 772
SseBI AGGCCT 1 cut(s) 467
SspMI CTAG 3 cut(s) 719, 942, 1492
SstI GAGCTC 1 cut(s) 567
StuI AGGCCT 1 cut(s) 467
StyD4I CCNGG 8 cut(s) 148, 234, 409, 467, 498, 1012, 1067, 1281
StyI CCWWGG 3 cut(s) 294, 300, 462
TaaI ACNGT 3 cut(s) 361, 751, 958
TaiI ACGT 2 cut(s) 345, 545
TaqII GACCGA 1 cut(s) 1283
TasI AATT 5 cut(s) 5, 509, 568, 609, 772
TatI WGTACW 3 cut(s) 721, 765, 855
TauI GCSGC 2 cut(s) 389, 398
TfiI GAWTC 3 cut(s) 687, 973, 1201
Tru1I TTAA 2 cut(s) 909, 1083
Tru9I TTAA 2 cut(s) 909, 1083
TscAI CASTG 1 cut(s) 997
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 6 cut(s) 202, 211, 899, 1153, 1171, 1318
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 3 cut(s) 17, 107, 182
TspRI CASTG 1 cut(s) 997
Van91I CCANNNNNTGG 1 cut(s) 468
VpaK11BI GGWCC 6 cut(s) 172, 226, 454, 459, 616, 1266
XapI RAATTY 1 cut(s) 509
XceI RCATGY 1 cut(s) 1178
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 3 cut(s) 719, 942, 1492
ZraI GACGTC 1 cut(s) 343
ZrmI AGTACT 2 cut(s) 723, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.