RLG00000010417
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015028
Physical Location & Seq
Reverse (-)
1452470 .. 1454002
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010417

Sequence Viewer

Length: 1533 bp
ATGAAATTGAAACCCATACCCAACCCGGACCCAGACCCAGACCAACCCCACCTCTCCTACATCTACTCCTTCCTCCACTCCTCTCTCCACCACCAAATGAACAATGATGCCGCCAAGAAACGCCGTCGTAAGACCGCCGACGACGACGGCCCGCCGGGAAAGCAAGGCAAGATGAAGGACCTGAAGGGGATCATCACGACCCTGTCGCTGCTGGAGCAGCAGGAGAAGGAGGACCTCGACCGGGCCTCAAATGACGACAAGTCGTTTGCGGAAGAGAAGCAGAAGCAGACGACGAAGACCAAGGCCATGGTGGAGTACTACTCCGATTTGCAGGGCTACTACTCGGACGTCGAGGAGTCCGAGACCGTGAAGCGGAAGAAGTCGAGAAACTCGGCGGCCGCGGCAGTTGCGACTGCGGCGGTTGACGCCTCCGAAAAGGGCGTGAAACCCGGAGGAAGCGGGTCGGGTCACCACCGGAGGCTTTGGGTCAAGGACCGGTCCAAGGCCTGGTGGGACGAGTGTAACCAACCGGATTTCCCGGAAGAGGAATTCAAAAAAGCCTTCCGGATGGGGAAGGCCACGTTCGATTTGATCTGCGAGGAGCTCAATTCGGCTATAGCGAAAGAGGACACCACTCTCCGGAATGCAATTCCGGTCCGGCAGAGAGTCGCGGTCTGTTTGTGGAGGTTAGCTACCGGCGACCCGCTCCGGCTCGTTTCGAAGCGATTCGGGTTAGGGATATCGACTTGTCATAAACTAGTACTCGAAGTCTGCTCAGCTATTAGGACTGTGTTGATGCCCAAGTACTTGAATTGGCCGGAGGATGGCGTTTTGAGGAAGGTGAAGGAGGAGTTCGAGACGTTTTCGGGTATACCTAATGTGGTAGGGTCTATGTACACTACTCATATTCCGATCATAGCTCCGAAGATTAGTGTGGCAGCTTATTTTAACAAGAGGCATACAGAGAGGAACCAGAAGACTAGCTACTCCATCACTGTGCAAGGTGTGGTGAATCCGAAAGGGGTGTTCACTGATGTGTGTATAGGGTGGCCTGGTTCAATGCCGGATGATCAGGTTCTGGAGAAGTCGGCTTTGCATCAGAGGGCGGGGAATGGTTTGCTTAAAGGTGTGTGGATTGTGGGGGGTTCGGGGTACCCTTTGCTGGATTGGGTGCTGGTGCCGTATAACCAGCAGCATCTTACTTGGACGCAGCATGCGTTCAATGAGAAGATTGGGGAGATTCAGAATGTGTCCAAAGATGCGTTTGCGAGGTTGAAAGGGAGGTGGTTGTGCTTGCAGAAGAGGACGGAGGTGAAGCTCCAGGACTTGCCTGTGGTTCTTGGGGCTTGCTGTGTGCTCCACAATATATGCGAGCTCAGGAATGAGGAAATCGACCCCGAGCTGAGGTTTGAGCTTGTGGATGATGACATGGTTCCGGAGCTTGCTTTGAGATCACAAAGCTCAATGAAGGCGAGGGATGCCATTGCTCATAATCTTTTGCATCATGGCCTTGCAGGCACTTCTTTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

511

Amino Acids

57.37

Weight (kDa)

8.5

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 299 - 455 7.9e-35 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
Acc65I GGTACC 1 cut(s) 1152
AccB1I GGYRCC 2 cut(s) 1152, 1177
AccB7I CCANNNNNTGG 1 cut(s) 507
AccBSI CCGCTC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 871
AccII CGCG 2 cut(s) 401, 671
AccIII TCCGGA 3 cut(s) 564, 639, 1435
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 2 cut(s) 396, 815
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 203
AcyI GRCGYC 2 cut(s) 348, 426
AfaI GTAC 5 cut(s) 317, 762, 806, 896, 1154
AfiI CCNNNNNNNGG 9 cut(s) 241, 372, 502, 507, 544, 639, 824, 1162, 1404
AgeI ACCGGT 1 cut(s) 495
AgsI TTSAA 6 cut(s) 10, 553, 811, 1059, 1222, 1276
AhdI GACNNNNNGTC 1 cut(s) 259
AhlI ACTAGT 1 cut(s) 757
AjnI CCWGG 3 cut(s) 506, 1051, 1320
AleI CACNNNNGTG 1 cut(s) 1034
Alw21I GWGCWC 3 cut(s) 606, 1359, 1377
Alw26I GTCTC 2 cut(s) 356, 851
AlwI GGATC 1 cut(s) 197
AlwNI CAGNNNCTG 1 cut(s) 1078
Ama87I CYCGRG 1 cut(s) 1397
Aor13HI TCCGGA 3 cut(s) 564, 639, 1435
AoxI GGCC 9 cut(s) 148, 243, 303, 396, 504, 576, 815, 1049, 1507
ApeKI GCWGC 5 cut(s) 208, 217, 938, 1192, 1210
ApoI RAATTY 1 cut(s) 548
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AsiGI ACCGGT 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 725
Asp718I GGTACC 1 cut(s) 1152
AspS9I GGNCC 8 cut(s) 28, 149, 178, 232, 243, 493, 498, 655
AsuC2I CCSGG 5 cut(s) 26, 156, 242, 450, 539
AsuHPI GGTGA 4 cut(s) 461, 853, 1021, 1324
AsuII TTCGAA 1 cut(s) 719
AvaI CYCGRG 1 cut(s) 1397
AvaII GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
BanI GGYRCC 2 cut(s) 1152, 1177
BanII GRGCYC 2 cut(s) 606, 1377
BarI GAAGNNNNNNTAC 2 cut(s) 968, 1000
BbsI GAAGAC 2 cut(s) 302, 983
Bbv12I GWGCWC 3 cut(s) 606, 1359, 1377
BbvCI CCTCAGC 1 cut(s) 1403
BbvI GCAGC 5 cut(s) 195, 229, 950, 1204, 1222
BccI CCATC 3 cut(s) 562, 818, 998
BceAI ACGGC 3 cut(s) 108, 163, 1165
BciT130I CCWGG 3 cut(s) 508, 1053, 1322
BclI TGATCA 1 cut(s) 1069
BcnI CCSGG 5 cut(s) 26, 156, 242, 450, 539
BcoDI GTCTC 2 cut(s) 356, 851
BcuI ACTAGT 1 cut(s) 757
BfaI CTAG 3 cut(s) 758, 981, 1531
BfmI CTRYAG 1 cut(s) 615
BglI GCCNNNNNGGC 1 cut(s) 1515
BlpI GCTNAGC 1 cut(s) 775
BmcAI AGTACT 3 cut(s) 317, 762, 806
Bme1390I CCNGG 8 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1322
Bme18I GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
BmeRI GACNNNNNGTC 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 8 cut(s) 28, 149, 178, 232, 243, 493, 498, 655
BmiI GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
BmrFI CCNGG 8 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1322
BmsI GCATC 7 cut(s) 97, 786, 1105, 1204, 1249, 1468, 1510
BpiI GAAGAC 2 cut(s) 302, 983
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 3 cut(s) 233, 1100, 1304
Bpu10I CCTNAGC 2 cut(s) 1376, 1403
Bpu1102I GCTNAGC 1 cut(s) 775
Bpu14I TTCGAA 1 cut(s) 719
BpuMI CCSGG 5 cut(s) 26, 156, 242, 450, 539
BsaHI GRCGYC 2 cut(s) 348, 426
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 300, 306, 399, 501
BsaWI WCCGGW 7 cut(s) 474, 495, 529, 564, 639, 652, 1435
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 9 cut(s) 241, 372, 502, 507, 544, 639, 824, 1162, 1404
Bse118I RCCGGY 2 cut(s) 495, 695
Bse3DI GCAATG 1 cut(s) 1482
BseAI TCCGGA 3 cut(s) 564, 639, 1435
BseBI CCWGG 3 cut(s) 508, 1053, 1322
BseDI CCNNGG 4 cut(s) 300, 306, 399, 501
BseGI GGATG 5 cut(s) 573, 829, 1072, 1426, 1483
BseLI CCNNNNNNNGG 9 cut(s) 241, 372, 502, 507, 544, 639, 824, 1162, 1404
BseMI GCAATG 1 cut(s) 1482
BseMII CTCAG 3 cut(s) 789, 1390, 1394
BseRI GAGGAG 4 cut(s) 70, 368, 614, 863
BseX3I CGGCCG 1 cut(s) 396
BseXI GCAGC 5 cut(s) 195, 229, 950, 1204, 1222
Bsh1236I CGCG 2 cut(s) 401, 671
Bsh1285I CGRYCG 2 cut(s) 241, 399
BshFI GGCC 9 cut(s) 150, 245, 305, 398, 506, 578, 817, 1051, 1509
BshNI GGYRCC 2 cut(s) 1152, 1177
BshTI ACCGGT 1 cut(s) 495
BsiEI CGRYCG 2 cut(s) 241, 399
BsiHKAI GWGCWC 3 cut(s) 606, 1359, 1377
BsiHKCI CYCGRG 1 cut(s) 1397
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 9 cut(s) 241, 372, 502, 507, 544, 639, 824, 1162, 1404
BsmAI GTCTC 2 cut(s) 356, 851
BsmBI CGTCTC 1 cut(s) 851
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 9 cut(s) 150, 245, 305, 398, 506, 578, 817, 1051, 1509
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 1397
Bsp119I TTCGAA 1 cut(s) 719
Bsp1286I GDGCHC 3 cut(s) 606, 1359, 1377
Bsp13I TCCGGA 3 cut(s) 564, 639, 1435
Bsp1407I TGTACA 1 cut(s) 894
Bsp143I GATC 5 cut(s) 189, 591, 912, 1069, 1451
Bsp1720I GCTNAGC 1 cut(s) 775
Bsp19I CCATGG 1 cut(s) 306
BspANI GGCC 9 cut(s) 150, 245, 305, 398, 506, 578, 817, 1051, 1509
BspCNI CTCAG 3 cut(s) 788, 1389, 1395
BspEI TCCGGA 3 cut(s) 564, 639, 1435
BspFNI CGCG 2 cut(s) 401, 671
BspLI GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
BspPI GGATC 1 cut(s) 197
BspT104I TTCGAA 1 cut(s) 719
BspT107I GGYRCC 2 cut(s) 1152, 1177
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 1482
BsrFI RCCGGY 2 cut(s) 495, 695
BsrGI TGTACA 1 cut(s) 894
BssAI RCCGGY 2 cut(s) 495, 695
BssECI CCNNGG 4 cut(s) 300, 306, 399, 501
BssMI GATC 5 cut(s) 189, 591, 912, 1069, 1451
BssNAI GTATAC 1 cut(s) 872
BssNI GRCGYC 2 cut(s) 348, 426
BssT1I CCWWGG 3 cut(s) 300, 306, 501
Bst1107I GTATAC 1 cut(s) 872
Bst2UI CCWGG 3 cut(s) 508, 1053, 1322
Bst4CI ACNGT 3 cut(s) 367, 790, 997
Bst6I CTCTTC 3 cut(s) 267, 537, 1295
BstACI GRCGYC 2 cut(s) 348, 426
BstAUI TGTACA 1 cut(s) 894
BstBI TTCGAA 1 cut(s) 719
BstC8I GCNNGC 7 cut(s) 152, 1215, 1295, 1348, 1373, 1443, 1516
BstDEI CTNAG 3 cut(s) 775, 1376, 1403
BstDSI CCRYGG 2 cut(s) 306, 399
BstEII GGTNACC 1 cut(s) 467
BstF5I GGATG 5 cut(s) 573, 829, 1072, 1426, 1483
BstFNI CGCG 2 cut(s) 401, 671
BstKTI GATC 5 cut(s) 192, 594, 915, 1072, 1454
BstMAI GTCTC 2 cut(s) 356, 851
BstMBI GATC 5 cut(s) 189, 591, 912, 1069, 1451
BstMCI CGRYCG 2 cut(s) 241, 399
BstMWI GCNNNNNNNGC 9 cut(s) 160, 214, 217, 401, 407, 416, 425, 1478, 1515
BstNI CCWGG 3 cut(s) 508, 1053, 1322
BstNSI RCATGY 1 cut(s) 1217
BstPI GGTNACC 1 cut(s) 467
BstSCI CCNGG 8 cut(s) 24, 154, 240, 448, 506, 537, 1051, 1320
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 2 cut(s) 401, 671
BstV1I GCAGC 5 cut(s) 195, 229, 950, 1204, 1222
BstV2I GAAGAC 2 cut(s) 302, 983
BstXI CCANNNNNNTGG 1 cut(s) 307
BstZ17I GTATAC 1 cut(s) 872
BstZI CGGCCG 1 cut(s) 396
BsuRI GGCC 9 cut(s) 150, 245, 305, 398, 506, 578, 817, 1051, 1509
BtgI CCRYGG 2 cut(s) 306, 399
BtsCI GGATG 5 cut(s) 573, 829, 1072, 1426, 1483
BtsIMutI CAGTG 2 cut(s) 993, 1029
Cac8I GCNNGC 7 cut(s) 152, 1215, 1295, 1348, 1373, 1443, 1516
CaiI CAGNNNCTG 1 cut(s) 1078
CciNI GCGGCCGC 1 cut(s) 396
Cfr10I RCCGGY 2 cut(s) 495, 695
Cfr13I GGNCC 8 cut(s) 28, 149, 178, 232, 243, 493, 498, 655
Cfr42I CCGCGG 1 cut(s) 402
CpoI CGGWCCG 1 cut(s) 655
CseI GACGC 2 cut(s) 434, 1216
Csp6I GTAC 5 cut(s) 316, 761, 805, 895, 1153
CspAI ACCGGT 1 cut(s) 495
CspI CGGWCCG 1 cut(s) 655
CviAII CATG 4 cut(s) 307, 1214, 1429, 1505
CviQI GTAC 5 cut(s) 316, 761, 805, 895, 1153
DdeI CTNAG 3 cut(s) 775, 1376, 1403
DpnI GATC 5 cut(s) 191, 593, 914, 1071, 1453
DpnII GATC 5 cut(s) 189, 591, 912, 1069, 1451
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 2 cut(s) 396, 815
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 267, 537, 1295
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 3 cut(s) 267, 537, 1295
Ecl136II GAGCTC 2 cut(s) 604, 1375
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 3 cut(s) 300, 306, 501
Eco147I AGGCCT 1 cut(s) 506
Eco24I GRGCYC 2 cut(s) 606, 1377
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 741
Eco47I GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
Eco52I CGGCCG 1 cut(s) 396
Eco53kI GAGCTC 2 cut(s) 604, 1375
Eco57I CTGAAG 1 cut(s) 203
Eco88I CYCGRG 1 cut(s) 1397
Eco91I GGTNACC 1 cut(s) 467
EcoICRI GAGCTC 2 cut(s) 604, 1375
EcoO109I RGGNCCY 2 cut(s) 178, 232
EcoO65I GGTNACC 1 cut(s) 467
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 506, 1051, 1320
EcoRV GATATC 1 cut(s) 741
EcoT14I CCWWGG 3 cut(s) 300, 306, 501
EcoT38I GRGCYC 2 cut(s) 606, 1377
ErhI CCWWGG 3 cut(s) 300, 306, 501
Esp3I CGTCTC 1 cut(s) 851
FaeI CATG 4 cut(s) 310, 1217, 1432, 1508
FaqI GGGAC 1 cut(s) 527
FatI CATG 4 cut(s) 306, 1213, 1428, 1504
FauI CCCGC 4 cut(s) 159, 452, 711, 1099
FbaI TGATCA 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 871
FokI GGATG 5 cut(s) 580, 836, 1079, 1433, 1490
FriOI GRGCYC 2 cut(s) 606, 1377
FspBI CTAG 3 cut(s) 758, 981, 1531
GsuI CTGGAG 3 cut(s) 233, 1100, 1304
HaeIII GGCC 9 cut(s) 150, 245, 305, 398, 506, 578, 817, 1051, 1509
HgaI GACGC 2 cut(s) 434, 1216
Hin1I GRCGYC 2 cut(s) 348, 426
Hin1II CATG 4 cut(s) 310, 1217, 1432, 1508
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 5 cut(s) 356, 666, 726, 1012, 1240
HphI GGTGA 4 cut(s) 461, 853, 1021, 1324
Hpy166II GTNNAC 4 cut(s) 424, 872, 897, 1029
Hpy188I TCNGA 9 cut(s) 325, 346, 361, 433, 912, 924, 1017, 1101, 1245
Hpy188III TCNNGA 8 cut(s) 196, 384, 565, 640, 856, 1079, 1378, 1436
Hpy8I GTNNAC 4 cut(s) 424, 872, 897, 1029
Hpy99I CGWCG 6 cut(s) 129, 143, 146, 149, 295, 353
HpyAV CCTTC 9 cut(s) 79, 169, 178, 220, 568, 571, 832, 838, 1462
HpyCH4III ACNGT 3 cut(s) 367, 790, 997
HpyCH4IV ACGT 3 cut(s) 348, 581, 860
HpyCH4V TGCA 7 cut(s) 331, 647, 1000, 1096, 1297, 1501, 1514
HpyF10VI GCNNNNNNNGC 9 cut(s) 160, 214, 217, 401, 407, 416, 425, 1478, 1515
HpyF3I CTNAG 3 cut(s) 775, 1376, 1403
HpySE526I ACGT 3 cut(s) 348, 581, 860
Hsp92I GRCGYC 2 cut(s) 348, 426
Hsp92II CATG 4 cut(s) 310, 1217, 1432, 1508
Kpn2I TCCGGA 3 cut(s) 564, 639, 1435
KpnI GGTACC 1 cut(s) 1156
Ksp22I TGATCA 1 cut(s) 1069
KspI CCGCGG 1 cut(s) 402
Kzo9I GATC 5 cut(s) 189, 591, 912, 1069, 1451
LmnI GCTCC 7 cut(s) 214, 601, 711, 925, 1323, 1362, 1438
Lsp1109I GCAGC 5 cut(s) 195, 229, 950, 1204, 1222
LweI GCATC 7 cut(s) 97, 786, 1105, 1204, 1249, 1468, 1510
MaeI CTAG 3 cut(s) 758, 981, 1531
MaeII ACGT 3 cut(s) 348, 581, 860
MaeIII GTNAC 2 cut(s) 467, 521
MalI GATC 5 cut(s) 191, 593, 914, 1071, 1453
MbiI CCGCTC 1 cut(s) 706
MboI GATC 5 cut(s) 189, 591, 912, 1069, 1451
MboII GAAGA 8 cut(s) 284, 307, 388, 554, 937, 988, 1240, 1312
MhlI GDGCHC 3 cut(s) 606, 1359, 1377
MluCI AATT 5 cut(s) 5, 548, 607, 648, 811
MlyI GAGTC 2 cut(s) 365, 675
MroI TCCGGA 3 cut(s) 564, 639, 1435
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 2 cut(s) 948, 1122
MslI CAYNNNNRTG 2 cut(s) 995, 1034
MspA1I CMGCKG 1 cut(s) 401
MspR9I CCNGG 8 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1322
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 3 cut(s) 508, 1053, 1322
MvnI CGCG 2 cut(s) 401, 671
MwoI GCNNNNNNNGC 9 cut(s) 160, 214, 217, 401, 407, 416, 425, 1478, 1515
NciI CCSGG 5 cut(s) 26, 156, 242, 450, 539
NcoI CCATGG 1 cut(s) 306
NdeII GATC 5 cut(s) 189, 591, 912, 1069, 1451
NlaIII CATG 4 cut(s) 310, 1217, 1432, 1508
NlaIV GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
NmeAIII GCCGAG 1 cut(s) 371
NmuCI GTSAC 1 cut(s) 467
NotI GCGGCCGC 1 cut(s) 396
NspI RCATGY 1 cut(s) 1217
NspV TTCGAA 1 cut(s) 719
OliI CACNNNNGTG 1 cut(s) 1034
PaeI GCATGC 1 cut(s) 1217
PceI AGGCCT 1 cut(s) 506
PcsI WCGNNNNNNNCGW 3 cut(s) 357, 617, 1214
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 3 cut(s) 726, 1012, 1240
PflFI GACNNNGTC 1 cut(s) 202
PflMI CCANNNNNTGG 1 cut(s) 507
PfoI TCCNGGA 2 cut(s) 537, 1320
PinAI ACCGGT 1 cut(s) 495
PleI GAGTC 2 cut(s) 364, 674
PpsI GAGTC 2 cut(s) 364, 674
PpuMI RGGWCCY 2 cut(s) 178, 232
Psp124BI GAGCTC 2 cut(s) 606, 1377
Psp5II RGGWCCY 2 cut(s) 178, 232
Psp6I CCWGG 3 cut(s) 506, 1051, 1320
PspEI GGTNACC 1 cut(s) 467
PspGI CCWGG 3 cut(s) 506, 1051, 1320
PspN4I GGNNCC 5 cut(s) 30, 971, 1154, 1179, 1434
PspPI GGNCC 8 cut(s) 28, 149, 178, 232, 243, 493, 498, 655
PspPPI RGGWCCY 2 cut(s) 178, 232
PstNI CAGNNNCTG 1 cut(s) 1078
PsyI GACNNNGTC 1 cut(s) 202
RsaI GTAC 5 cut(s) 317, 762, 806, 896, 1154
RsaNI GTAC 5 cut(s) 316, 761, 805, 895, 1153
RseI CAYNNNNRTG 2 cut(s) 995, 1034
Rsr2I CGGWCCG 1 cut(s) 655
RsrII CGGWCCG 1 cut(s) 655
SacI GAGCTC 2 cut(s) 606, 1377
SacII CCGCGG 1 cut(s) 402
SaqAI TTAA 2 cut(s) 948, 1122
Sau3AI GATC 5 cut(s) 189, 591, 912, 1069, 1451
Sau96I GGNCC 8 cut(s) 28, 149, 178, 232, 243, 493, 498, 655
ScaI AGTACT 3 cut(s) 317, 762, 806
SchI GAGTC 2 cut(s) 365, 675
ScrFI CCNGG 8 cut(s) 26, 156, 242, 450, 508, 539, 1053, 1322
SduI GDGCHC 3 cut(s) 606, 1359, 1377
SfaNI GCATC 7 cut(s) 97, 786, 1105, 1204, 1249, 1468, 1510
SfcI CTRYAG 1 cut(s) 615
Sfr303I CCGCGG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 719
SgrBI CCGCGG 1 cut(s) 402
SinI GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
SmiMI CAYNNNNRTG 2 cut(s) 995, 1034
SpeI ACTAGT 1 cut(s) 757
SphI GCATGC 1 cut(s) 1217
Sse9I AATT 5 cut(s) 5, 548, 607, 648, 811
SseBI AGGCCT 1 cut(s) 506
SspMI CTAG 3 cut(s) 758, 981, 1531
SstI GAGCTC 2 cut(s) 606, 1377
StuI AGGCCT 1 cut(s) 506
StyD4I CCNGG 8 cut(s) 24, 154, 240, 448, 506, 537, 1051, 1320
StyI CCWWGG 3 cut(s) 300, 306, 501
TaaI ACNGT 3 cut(s) 367, 790, 997
TaiI ACGT 3 cut(s) 351, 584, 863
TaqI TCGA 9 cut(s) 237, 351, 383, 585, 719, 743, 765, 855, 1392
TasI AATT 5 cut(s) 5, 548, 607, 648, 811
TatI WGTACW 4 cut(s) 315, 760, 804, 894
TauI GCSGC 5 cut(s) 113, 398, 401, 404, 419
TfiI GAWTC 3 cut(s) 726, 1012, 1240
Tru1I TTAA 2 cut(s) 948, 1122
Tru9I TTAA 2 cut(s) 948, 1122
TscAI CASTG 2 cut(s) 1000, 1036
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 5 cut(s) 208, 217, 938, 1192, 1210
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 4 cut(s) 17, 113, 188, 1481
TspGWI ACGGA 1 cut(s) 1322
TspRI CASTG 2 cut(s) 1000, 1036
Tth111I GACNNNGTC 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 507
VpaK11BI GGWCC 6 cut(s) 28, 178, 232, 493, 498, 655
XapI RAATTY 1 cut(s) 548
XceI RCATGY 1 cut(s) 1217
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 871
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 3 cut(s) 758, 981, 1531
ZraI GACGTC 1 cut(s) 349
ZrmI AGTACT 3 cut(s) 317, 762, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.