Rmu_co8316609.1_g000001
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8316609.1
OG0015028
Physical Location & Seq
Forward (+)
1 .. 935
935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8316609.1_g000001.1.cds

Sequence Viewer

Length: 935 bp
aagtcgagaaacgcggcgaccgcggccgttgcggttgcggcggcggcggttggggcctccgaaaagggcgcgaaacccggaggaagcgggtcgggtcaccagcggaggctttgggtcaaggaccggtccaaggcctggtgggacgagtgtaaccaacccgatttcccggaagaggaattcagaaaggccttccggatggggaaggccacgttcgatttgatctgcgacgagctcaattcggctatagcgaaagaggacaccactctccggaatgcaattccggtccggcagagagtcgccgtctgtttgtggaggttagctaccggcgacccgctccggctcgtttcgaagcgattcgggttagggatatcgacttgtcataagctagtactcgaagtctgctcagctattaggactgtgttgatgcccaagtacttgaattggccggaggatggtgttttgaggaaggtgaaggacgagttcgagtcgttttcgggtatacctaatgttgtagggtctatgtacactactcatattccgatcatagctccgaagattagtgtggcagcttattttaacaagaggcatacagagaggaaccagaagactagctactccatcaccgtgcaaggtgtggtgaatccgaaaggggtgttcactgatgtgtgtatagggtggcctggttcaatgccggatgatcaggttctggagaagtcggctttgcatcagagggccgggaatggtctgcttaaaggtgtgtggattgtggggggttcggggtaccctttgctggattgggtgctggtgccctatactcagcagcatcttacttggacgcagcatgcgttcaatgagaagattggggagattcagaatgtgtccaaagatgcgtttgcgaggttgaaggggaggtggttgtgcttgcagaagaggac
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

312

Amino Acids

34.66

Weight (kDa)

9.54

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015028)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12010
fragaria_vesca FvH4_2g31471
malus_domestica MD15G1055200.v1.1
prunus_persica Prupe.1G408400_v2.0.a1
pyrus_communis pycom08g05470 pycom15g05210
rosa_chinensis RchiOBHm_Chr6g0311321
rosa_laevigata RLG00000010417
rosa_multiflora Rmu_co8316609.1_g000001
rosa_roxburghii Rroxscaffold_7G00157760
rosa_rugosa Rorug06G0387200
rosa_samantha Rh6AG502300 Rh6BG512900 Rh6CG518600 Rh6DG504900
rosa_wichuraiana Rw6G043730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 780, 805
AccB7I CCANNNNNTGG 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 499
AccII CGCG 3 cut(s) 14, 23, 71
AccIII TCCGGA 2 cut(s) 192, 267
AcoI YGGCCR 2 cut(s) 24, 443
AcsI RAATTY 1 cut(s) 176
AfaI GTAC 4 cut(s) 390, 434, 524, 782
AfiI CCNNNNNNNGG 6 cut(s) 130, 135, 172, 267, 452, 790
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 4 cut(s) 439, 687, 850, 904
AjnI CCWGG 2 cut(s) 134, 679
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 7 cut(s) 232, 320, 385, 407, 548, 569, 612
AluI AGCT 7 cut(s) 232, 320, 385, 407, 548, 569, 612
Alw21I GWGCWC 1 cut(s) 234
AlwNI CAGNNNCTG 1 cut(s) 706
Aor13HI TCCGGA 2 cut(s) 192, 267
AoxI GGCC 8 cut(s) 24, 54, 132, 186, 204, 443, 677, 732
ApeKI GCWGC 3 cut(s) 566, 820, 838
ApoI RAATTY 1 cut(s) 176
AsiGI ACCGGT 1 cut(s) 123
Asp700I GAANNNNTTC 1 cut(s) 353
Asp718I GGTACC 1 cut(s) 780
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 5 cut(s) 54, 121, 126, 283, 732
AsuC2I CCSGG 3 cut(s) 78, 167, 736
AsuHPI GGTGA 4 cut(s) 89, 481, 613, 649
AsuII TTCGAA 1 cut(s) 347
AvaII GGWCC 3 cut(s) 121, 126, 283
BaeGI GKGCMC 1 cut(s) 810
BanI GGYRCC 2 cut(s) 780, 805
BanII GRGCYC 1 cut(s) 234
BarI GAAGNNNNNNTAC 2 cut(s) 596, 628
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 578, 832, 850
BccI CCATC 3 cut(s) 190, 446, 626
BceAI ACGGC 2 cut(s) 11, 284
BciT130I CCWGG 2 cut(s) 136, 681
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 3 cut(s) 78, 167, 736
BfaI CTAG 2 cut(s) 386, 609
BfmI CTRYAG 1 cut(s) 243
BisI GCNGC 8 cut(s) 15, 24, 39, 42, 45, 567, 821, 839
BlpI GCTNAGC 1 cut(s) 403
BlsI GCNGC 8 cut(s) 16, 25, 40, 43, 46, 568, 822, 840
BmcAI AGTACT 2 cut(s) 390, 434
Bme1390I CCNGG 5 cut(s) 78, 136, 167, 681, 736
Bme18I GGWCC 3 cut(s) 121, 126, 283
BmgT120I GGNCC 5 cut(s) 54, 121, 126, 283, 732
BmiI GGNNCC 4 cut(s) 55, 599, 782, 807
BmrFI CCNGG 5 cut(s) 78, 136, 167, 681, 736
BmsI GCATC 4 cut(s) 414, 733, 832, 877
BpiI GAAGAC 1 cut(s) 611
BpmI CTGGAG 1 cut(s) 728
Bpu1102I GCTNAGC 1 cut(s) 403
Bpu14I TTCGAA 1 cut(s) 347
BpuMI CCSGG 3 cut(s) 78, 167, 736
BsaJI CCNNGG 2 cut(s) 21, 129
BsaWI WCCGGW 4 cut(s) 123, 192, 267, 280
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 135, 172, 267, 452, 790
Bse118I RCCGGY 2 cut(s) 123, 323
BseAI TCCGGA 2 cut(s) 192, 267
BseBI CCWGG 2 cut(s) 136, 681
BseDI CCNNGG 2 cut(s) 21, 129
BseGI GGATG 3 cut(s) 201, 457, 700
BseLI CCNNNNNNNGG 6 cut(s) 130, 135, 172, 267, 452, 790
BseMII CTCAG 2 cut(s) 417, 830
BseSI GKGCMC 1 cut(s) 810
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 3 cut(s) 578, 832, 850
Bsh1236I CGCG 3 cut(s) 14, 23, 71
Bsh1285I CGRYCG 2 cut(s) 21, 27
BshFI GGCC 8 cut(s) 26, 56, 134, 188, 206, 445, 679, 734
BshNI GGYRCC 2 cut(s) 780, 805
BshTI ACCGGT 1 cut(s) 123
BsiEI CGRYCG 2 cut(s) 21, 27
BsiHKAI GWGCWC 1 cut(s) 234
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 6 cut(s) 130, 135, 172, 267, 452, 790
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 8 cut(s) 26, 56, 134, 188, 206, 445, 679, 734
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 2 cut(s) 234, 810
Bsp13I TCCGGA 2 cut(s) 192, 267
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 3 cut(s) 219, 540, 697
Bsp1720I GCTNAGC 1 cut(s) 403
BspANI GGCC 8 cut(s) 26, 56, 134, 188, 206, 445, 679, 734
BspCNI CTCAG 2 cut(s) 416, 829
BspEI TCCGGA 2 cut(s) 192, 267
BspFNI CGCG 3 cut(s) 14, 23, 71
BspLI GGNNCC 4 cut(s) 55, 599, 782, 807
BspT104I TTCGAA 1 cut(s) 347
BspT107I GGYRCC 2 cut(s) 780, 805
BsrBI CCGCTC 1 cut(s) 334
BsrFI RCCGGY 2 cut(s) 123, 323
BsrGI TGTACA 1 cut(s) 522
BssAI RCCGGY 2 cut(s) 123, 323
BssECI CCNNGG 2 cut(s) 21, 129
BssMI GATC 3 cut(s) 219, 540, 697
BssNAI GTATAC 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 129
Bst1107I GTATAC 1 cut(s) 500
Bst2UI CCWGG 2 cut(s) 136, 681
Bst4CI ACNGT 2 cut(s) 418, 625
Bst6I CTCTTC 2 cut(s) 165, 923
BstAUI TGTACA 1 cut(s) 522
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 843, 923
BstDEI CTNAG 2 cut(s) 403, 816
BstDSI CCRYGG 1 cut(s) 21
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 3 cut(s) 201, 457, 700
BstFNI CGCG 3 cut(s) 14, 23, 71
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 3 cut(s) 222, 543, 700
BstMBI GATC 3 cut(s) 219, 540, 697
BstMCI CGRYCG 2 cut(s) 21, 27
BstMWI GCNNNNNNNGC 6 cut(s) 20, 23, 29, 38, 44, 53
BstNI CCWGG 2 cut(s) 136, 681
BstNSI RCATGY 1 cut(s) 845
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 5 cut(s) 76, 134, 165, 679, 734
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 810
BstUI CGCG 3 cut(s) 14, 23, 71
BstV1I GCAGC 3 cut(s) 578, 832, 850
BstV2I GAAGAC 1 cut(s) 611
BstZ17I GTATAC 1 cut(s) 500
BstZI CGGCCG 1 cut(s) 24
BsuRI GGCC 8 cut(s) 26, 56, 134, 188, 206, 445, 679, 734
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 3 cut(s) 201, 457, 700
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 843, 923
CaiI CAGNNNCTG 1 cut(s) 706
CfoI GCGC 1 cut(s) 71
Cfr10I RCCGGY 2 cut(s) 123, 323
Cfr13I GGNCC 5 cut(s) 54, 121, 126, 283, 732
Cfr42I CCGCGG 1 cut(s) 24
CpoI CGGWCCG 1 cut(s) 283
CseI GACGC 1 cut(s) 844
Csp6I GTAC 4 cut(s) 389, 433, 523, 781
CspAI ACCGGT 1 cut(s) 123
CspI CGGWCCG 1 cut(s) 283
CviAII CATG 1 cut(s) 842
CviQI GTAC 4 cut(s) 389, 433, 523, 781
DdeI CTNAG 2 cut(s) 403, 816
DpnI GATC 3 cut(s) 221, 542, 699
DpnII GATC 3 cut(s) 219, 540, 697
EaeI YGGCCR 2 cut(s) 24, 443
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 165, 923
EarI CTCTTC 2 cut(s) 165, 923
Ecl136II GAGCTC 1 cut(s) 232
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 129
Eco147I AGGCCT 2 cut(s) 134, 188
Eco24I GRGCYC 1 cut(s) 234
Eco32I GATATC 1 cut(s) 369
Eco47I GGWCC 3 cut(s) 121, 126, 283
Eco52I CGGCCG 1 cut(s) 24
Eco53kI GAGCTC 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 134, 679
EcoRV GATATC 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 1 cut(s) 845
FaqI GGGAC 1 cut(s) 155
FatI CATG 1 cut(s) 841
FauI CCCGC 2 cut(s) 80, 339
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 8 cut(s) 15, 24, 39, 42, 45, 567, 821, 839
FokI GGATG 3 cut(s) 208, 464, 707
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 8 cut(s) 15, 24, 39, 42, 45, 567, 821, 839
FspBI CTAG 2 cut(s) 386, 609
GlaI GCGC 1 cut(s) 70
GluI GCNGC 8 cut(s) 15, 24, 39, 42, 45, 567, 821, 839
GsuI CTGGAG 1 cut(s) 728
HaeIII GGCC 8 cut(s) 26, 56, 134, 188, 206, 445, 679, 734
HgaI GACGC 1 cut(s) 844
HhaI GCGC 1 cut(s) 71
Hin1II CATG 1 cut(s) 845
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HinfI GANTC 5 cut(s) 294, 354, 485, 640, 868
HphI GGTGA 4 cut(s) 89, 481, 613, 649
Hpy166II GTNNAC 3 cut(s) 500, 525, 657
Hpy188I TCNGA 7 cut(s) 61, 182, 540, 552, 645, 729, 873
Hpy188III TCNNGA 4 cut(s) 6, 193, 268, 707
Hpy8I GTNNAC 3 cut(s) 500, 525, 657
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 5 cut(s) 196, 199, 460, 466, 898
HpyCH4III ACNGT 2 cut(s) 418, 625
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 4 cut(s) 275, 628, 724, 925
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 23, 29, 38, 44, 53
HpyF3I CTNAG 2 cut(s) 403, 816
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 1 cut(s) 845
HspAI GCGC 1 cut(s) 69
Kpn2I TCCGGA 2 cut(s) 192, 267
KpnI GGTACC 1 cut(s) 784
Ksp22I TGATCA 1 cut(s) 697
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 3 cut(s) 219, 540, 697
LmnI GCTCC 2 cut(s) 339, 553
Lsp1109I GCAGC 3 cut(s) 578, 832, 850
LweI GCATC 4 cut(s) 414, 733, 832, 877
MaeI CTAG 2 cut(s) 386, 609
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 2 cut(s) 95, 149
MalI GATC 3 cut(s) 221, 542, 699
MbiI CCGCTC 1 cut(s) 334
MboI GATC 3 cut(s) 219, 540, 697
MboII GAAGA 4 cut(s) 182, 565, 616, 868
MhlI GDGCHC 2 cut(s) 234, 810
MluCI AATT 4 cut(s) 176, 235, 276, 439
MlyI GAGTC 2 cut(s) 303, 494
MroI TCCGGA 2 cut(s) 192, 267
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 576, 750
MslI CAYNNNNRTG 2 cut(s) 623, 662
MspA1I CMGCKG 2 cut(s) 23, 103
MspR9I CCNGG 5 cut(s) 78, 136, 167, 681, 736
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 2 cut(s) 136, 681
MvnI CGCG 3 cut(s) 14, 23, 71
MwoI GCNNNNNNNGC 6 cut(s) 20, 23, 29, 38, 44, 53
NciI CCSGG 3 cut(s) 78, 167, 736
NdeII GATC 3 cut(s) 219, 540, 697
NlaIII CATG 1 cut(s) 845
NlaIV GGNNCC 4 cut(s) 55, 599, 782, 807
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 845
NspV TTCGAA 1 cut(s) 347
OliI CACNNNNGTG 1 cut(s) 662
PaeI GCATGC 1 cut(s) 845
PceI AGGCCT 2 cut(s) 134, 188
PcsI WCGNNNNNNNCGW 2 cut(s) 245, 842
PctI GAATGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 3 cut(s) 354, 640, 868
PflMI CCANNNNNTGG 1 cut(s) 135
PfoI TCCNGGA 1 cut(s) 165
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 8 cut(s) 16, 25, 40, 43, 46, 568, 822, 840
PleI GAGTC 2 cut(s) 302, 493
PpsI GAGTC 2 cut(s) 302, 493
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 134, 679
PspEI GGTNACC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 134, 679
PspN4I GGNNCC 4 cut(s) 55, 599, 782, 807
PspPI GGNCC 5 cut(s) 54, 121, 126, 283, 732
PstNI CAGNNNCTG 1 cut(s) 706
RsaI GTAC 4 cut(s) 390, 434, 524, 782
RsaNI GTAC 4 cut(s) 389, 433, 523, 781
RseI CAYNNNNRTG 2 cut(s) 623, 662
Rsr2I CGGWCCG 1 cut(s) 283
RsrII CGGWCCG 1 cut(s) 283
SacI GAGCTC 1 cut(s) 234
SacII CCGCGG 1 cut(s) 24
SaqAI TTAA 2 cut(s) 576, 750
SatI GCNGC 8 cut(s) 15, 24, 39, 42, 45, 567, 821, 839
Sau3AI GATC 3 cut(s) 219, 540, 697
Sau96I GGNCC 5 cut(s) 54, 121, 126, 283, 732
ScaI AGTACT 2 cut(s) 390, 434
SchI GAGTC 2 cut(s) 303, 494
ScrFI CCNGG 5 cut(s) 78, 136, 167, 681, 736
SduI GDGCHC 2 cut(s) 234, 810
SfaNI GCATC 4 cut(s) 414, 733, 832, 877
SfcI CTRYAG 1 cut(s) 243
Sfr303I CCGCGG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 347
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 3 cut(s) 121, 126, 283
SmiMI CAYNNNNRTG 2 cut(s) 623, 662
SphI GCATGC 1 cut(s) 845
Sse9I AATT 4 cut(s) 176, 235, 276, 439
SseBI AGGCCT 2 cut(s) 134, 188
SspMI CTAG 2 cut(s) 386, 609
SstI GAGCTC 1 cut(s) 234
StuI AGGCCT 2 cut(s) 134, 188
StyD4I CCNGG 5 cut(s) 76, 134, 165, 679, 734
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 418, 625
TaiI ACGT 1 cut(s) 212
TaqI TCGA 6 cut(s) 5, 213, 347, 371, 393, 483
TasI AATT 4 cut(s) 176, 235, 276, 439
TatI WGTACW 3 cut(s) 388, 432, 522
TauI GCSGC 5 cut(s) 17, 26, 41, 44, 47
TfiI GAWTC 3 cut(s) 354, 640, 868
Tru1I TTAA 2 cut(s) 576, 750
Tru9I TTAA 2 cut(s) 576, 750
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 566, 820, 838
Tsp45I GTSAC 1 cut(s) 95
TspRI CASTG 1 cut(s) 664
Van91I CCANNNNNTGG 1 cut(s) 135
VpaK11BI GGWCC 3 cut(s) 121, 126, 283
XapI RAATTY 1 cut(s) 176
XceI RCATGY 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 2 cut(s) 386, 609
ZrmI AGTACT 2 cut(s) 390, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.